| swinepox. virus isolation, experimental infections and the differentiation from vaccinia virus infections. | the isolation of swinepox virus in primary pig kidney cell cultures is reported. the differentiation from vaccinia virus was possible with challenge infections of convalescent pigs and the use of the agar gel diffusion precipitation (agdp) test and immuno-electroosmophoresis (ieop). using both immune precipitation tests reactions of identity were obtained between the heterologous antigens of swinepox and vaccinia viruses. a total of 829 pig sera from the field were tested for precipitating antib ... | 1975 | 174522 |
| ultrastructural aspects of experimental swinepox with special reference to inclusion bodies. | a light and electron microscopic study was performed on pox-like epidermal lesions in an experimentally infected pig. light microscopical investigation of semithin sections revealed the presence of nuclear vacuoles and of different types of cytoplasmic inclusions. in electron microscopical studies large numbers of both immature and mature virus particles and the cytological changes indicative of pox virus infection were observed. various types of intra-cytoplasmic inclusions -- i.e. fibrillar in ... | 1975 | 174523 |
| ultrastructural study of cell cultures infected with swinepox and orf viruses. | monolayer cells of a porcine kidney cell line were infected with the pp-1 strain of swinepox virus, while secondary or third subcultured monolayer cells of african green monkey kidney were inoculated with the iwate bt-9 strain of orf virus. those infected cells were fixed when cpe became remarkable in the monolayers and examined by electron microscope. in the cytoplasm of cells infected with both viruses, various immature forms of viral particles in different developmental stages were observed. ... | 1977 | 412491 |
| swinepox virus as a vector for the delivery of immunogens. | | 1991 | 1809192 |
| identification and nucleotide sequence of the thymidine kinase gene of swinepox virus. | using degenerative oligonucleotide probes, representing two different conserved regions of poxvirus and mammalian thymidine kinase (tk) genes, the swinepox virus (spv) tk gene was mapped to a 1.7-kb bamhi-hindiii fragment of the viral genome. nucleotide sequencing of this dna piece revealed that the spv tk gene was encoded by an open reading frame (orf) of 177 codons. immediately downstream of the tk gene was a second orf with homologues at the same location in both capripoxvirus and leporipoxvi ... | 1991 | 1840707 |
| the molecular biology of swinepox virus. ii. the infectious cycle. | studies based on low-stringency hybridizations of radiolabeled swinepox virus (spv) dna to southern blots containing dna of representative members of the orthopoxvirus, leporipoxvirus, and avipoxvirus genera and the entomopoxvirus subfamily have revealed no dna homology at this level of resolution. antigenic relatedness between spv and vaccinia was also analyzed using immunoprecipitations and revealed little if any cross-reactivity. the growth characteristics of spv in tissue culture were examin ... | 1991 | 1845830 |
| isolation and molecular characterization of the swinepox virus thymidine kinase gene. | swinepox virus (spv), the only member of the suipoxvirus genus, shows little antigenic relatedness or dna homology to members of the other poxvirus genera. a spv thymidine kinase (tk) gene was detected and mapped to the left end of the hindiii g fragment using degenerate oligonucleotide probes. cloning and sequencing of a 1.8-kb hindiii-bamhi fragment containing the spv tk gene revealed an open reading frame (orf) of 181 amino acids yielding a predicted polypeptide of mr 20.6 kda with significan ... | 1991 | 1853562 |
| the molecular biology of swinepox virus. i. a characterization of the viral dna. | swinepox virus (spv), the prototype member of the suipoxvirus genus, is uncharacterized at the molecular level. we have analyzed the dna of spv and demonstrate that the genome is 175 kb in size and like the more commonly studied orthopoxvirus, avipoxvirus, and leporipoxvirus genera, is terminally cross-linked and contains inverted terminal repetitions (itrs). in addition, the itrs are unstable, probably due to the presence of a variable number of direct repeats of approximately 70 bp in length. ... | 1991 | 1984655 |
| four sporadic cases of congenital swinepox. | four sporadic cases of congenital swinepox are described. the pigs were born with pox lesions over their entire bodies. microscopic examination revealed ballooning degeneration of keratinocytes, cytoplasmic inclusions and intranuclear vacuolisation. electron microscopy detected poxvirus particles in all four pigs. the virus was isolated from one pig and identified by hindiii restriction endonuclease digestion as swinepox virus. | 1990 | 2169139 |
| adaptation of swinepox virus to an established cell line. | swinepox virus was successfully adapted to the pk-15 cell line, but not the mdbk cell line. virus titers obtained in the pk-15 host cell system were comparable to diploid swine kidney cell cultures. | 1972 | 4110427 |
| studies on swinepox virus: fluorescence and light microscopy of infected cell cultures. | | 1973 | 4141785 |
| electron microscopy of swinepox virus in germfree pigs and in cell culture. | | 1971 | 4331896 |
| replication of swinepox virus in the skin of naturally infected pigs. electron microscopic study. | | 1973 | 4358765 |
| experimental induction of cutaneous streptococcal abscesses in swine as a sequela to swinepox. | streptococcal abscesses were experimentally induced in skin of swine as a sequela to swinepox. swinepox lesions were more uniform in distribution when infection was induced by iv injection of swinepox virus than when induced by lice (haematopinus suis) or scarification of skin. the incubation period for swinepox was between 10 and 14 days when virus was administered iv. cutaneous abscesses developed in swine given iv injection of streptococci 10 and 14 days after they were injected with swinepox ... | 1980 | 6245608 |
| the establishment of a genetic map of orf virus reveals a pattern of genomic organization that is highly conserved among divergent poxviruses. | the large differences between the g+c content of the orf virus genome and those of other characterized poxviruses have precluded the use of dna hybridization to establish a gene map of orf virus. here we have sequenced the ends of cloned restriction endonuclease fragments of the nz2 strain of orf virus (ov) and used the translated sequences to search protein data bases. sequence from 15 points found high-scoring matches to data base entries, including 18 vaccinia virus (vac) genes. we also prese ... | 1995 | 7571439 |
| unclassified poxvirus: characterization and physical mapping of cotia virus dna and location of a sequence capable of encoding a thymidine kinase. | dna from cotia virus, an unclassified poxvirus, was mapped by overlapping fragment analysis using the restriction endonucleases hindiii, psti, bamhi, xhoi, sali, and smai. the linear genome was 177 kbp in length and possessed inverted terminal repeats and cross-links. a cotia virus thymidine kinase (tk) gene was detected and mapped to about 74 kbp from the left end of the genome using degenerate oligonucleotide probes. nucleotide sequencing of the tk gene revealed an open reading frame (orf) tha ... | 1995 | 7793081 |
| evaluation of swinepox virus as a vaccine vector in pigs using an aujeszky's disease (pseudorabies) virus gene insert coding for glycoproteins gp50 and gp63. | pigs were vaccinated by scarification or intramuscular injection with a swinepox virus-aujeszky's disease (pseudorabies) recombinant (rspv-ad) constructed by inserting the linked aujeszky's disease virus genes coding for glycoproteins gp50 and gp63, attached to a vaccinia virus p7.5 promoter, into the thymidine kinase gene of swinepox virus. by 21 days after vaccination, 90 and 100 per cent of the animals vaccinated by scarification or intramuscular injection, respectively, had developed serum n ... | 1994 | 8128561 |
| dna sequence analysis of conserved and unique regions of swinepox virus: identification of genetic elements supporting phenotypic observations including a novel g protein-coupled receptor homologue. | swinepox virus (spv) contains a double-stranded cross-linked linear dna genome of approximately 175 kilobase pairs with terminal inverted repetitions (tirs) of 4.3 kb. the nucleotide sequence was determined for fragments from several regions of the genome including a 2.85-kb fragment from the central potentially conserved portion and two fragments within the presumed variable near-terminal regions which tend to be unique to a given poxvirus. the core sequence contains one partial and two complet ... | 1993 | 8249275 |
| potential viral vectors for the stimulation of mucosal antibody responses against enteric viral antigens in pigs. | four viruses were compared for their ability to induce an intestinal antibody response in piglets. antibodies were not detected in response to oral vaccination with either fowlpox virus or a baculovirus (bv). simultaneous oral dosing and parenteral inoculation with high concentrations of bv in an oil emulsion adjuvant induced high levels of circulating virus neutralising (vn) antibodies, and also low levels of intestinal antibodies when booster doses of virus were given. in response to oral vacc ... | 1993 | 8393209 |
| an entomopoxvirus homologue of the vaccinia virus d13l-encoded 'rifampicin resistance' protein. | the heliothis armigera entomopoxvirus (haepv) genome encodes a predicted 68 kda polypeptide related to the 'rifampicin resistance' protein of vaccinia virus (with 30 % identity), and an homologous swinepox virus protein (27% identity). we were unable to isolate an haepv genotypic variant encoding a predicted c-terminal truncated form of the protein, suggesting that the c terminus of the molecule may be essential to protein function, and, in turn, that this function may be essential to viral repl ... | 1996 | 8609479 |
| sequence and analysis of a swinepox virus homologue of the vaccinia virus major envelope protein p37 (f13l). | p37 (f13l gene product), the most abundant protein in the envelope of the extracellular virus form of the prototype poxvirus, vaccinia virus (vv), is a crucial player in the process leading to acquisition of the envelope, virus egress and transmission. we have cloned and sequenced a swinepox virus (spv) gene homologous to vv f13l. the spv gene product, termed p42, was 54% identical to p37, the vv f13l gene product, and, among the poxviruses, was most similar (73% identity) to the myxoma virus ho ... | 2000 | 10725435 |
| replication and expression of a swinepox virus vector delivering feline leukemia virus gag and env to cell lines of swine and feline origin. | the host range of swinepox virus (spv) is restricted to swine, although spv has been shown to infect mammalian, non-swine cells, without recovery of infectious virus. spv is a reasonable candidate for development as a non-productively replicating viral vector for use in non-swine, mammalian species, such as the cat. a novel spv gene deletion (spv 043) was created and found to be non-attenuating. this deletion was utilized to generate a stable recombinant virus expressing the gag-pro and env prot ... | 2003 | 14609625 |
| feline b7.1 and b7.2 proteins produced from swinepox virus vectors are natively processed and biologically active: potential for use as nonchemical adjuvants. | costimulatory ligands, b7.1 and b7.2, have been incorporated into viral and dna vectors as potential nonchemical adjuvants to enhance ctl and humoral immune responses against viral pathogens. in addition, soluble b7 proteins, minus their transmembrane and cytoplasmic domains, have been shown to block the down regulation of t-cell activation through blockade of b7/ctla-4 interactions in mouse tumor models. recently, we developed swinepox virus (spv) vectors for delivery of feline leukemia antigen ... | 2005 | 16181751 |
| comparative genetic analysis of genomic dna sequences of two human isolates of tanapox virus. | members of the genus yatapoxvirus, which include tanapox virus (tpv) and yaba monkey tumor virus, infect primates including humans. two strains of tpv isolated 50 years apart from patients infected from the equatorial region of africa have been sequenced. the original isolate from a human case in the tana river valley, kenya, in 1957 (tpv-kenya) and an isolate from an infected traveler in the republic of congo in 2004 (tpv-roc). although isolated 50 years apart the genomes were highly conserved. ... | 2007 | 17574698 |
| a novel vaccine against streptococcus equi ssp. zooepidemicus infections: the recombinant swinepox virus expressing m-like protein. | to develop a safer, more immunogenic and efficacious vaccine against streptococcus equi ssp. zooepidemicus (sez) infections, the gene of m-like protein (szp) was placed under the strong vaccinia virus promoter p28 and then inserted into swinepox virus (spv) genome. the recombinant swinepox virus (rspv-szp) was isolated in a non-selective medium by the co-expression of escherichia coli lacz gene and verified by pcr, western blotting and immunofluorescence assays. to evaluate the immunogenicity of ... | 2011 | 21807055 |
| immune responses and protective efficacy of a recombinant swinepox virus co-expressing ha1 genes of h3n2 and h1n1 swine influenza virus in mice and pigs. | the recombinant swine poxvirus rspv/h3-2a-h1 co-expressing ha1 genes of h3n2 and h1n1 subtype siv has been constructed and identified. inoculations of rspv/h3-2a-h1 yielded elisa and neutralization antibodies against siv h1n1 and h3n2, and elicited potent h1n1 and h3n2 siv-specific inf-γ response from t-lymphocytes in mice and pigs in this study. complete protection against siv h1n1 or h3n2 challenge in pigs was observed. | 2012 | 23265244 |
| one-step duplex polymerase chain reaction for the detection of swinepox and vaccinia viruses in skin lesions of swine with poxvirus-related disease. | infection of pigs with swinepox virus (swpv) was reported in brazil in 2011. swpv causes a systemic pustular disease in pigs and the symptoms are clinically indistinguishable from those caused by vaccinia virus (vacv) infection. pigs infected with vacv have been reported in various countries; however, vacv is endemic in brazil, india and other countries, where it affects mainly dairy cows, dairy buffaloes and dairy workers causing localized pustules. the transmission of vacv to other susceptible ... | 2015 | 25804245 |
| a poxvirus of primates. ii. immunology. | orteca poxvirus shares antigens with yaba virus, as evidenced by two-way crossreactions in complement-fixation and neutralization tests. antibodies produced by vaccinia and monkeypox viruses showed weak complement-fixation reactions with orteca viral antigen, but neutralization reactions were negative and cross protection in vivo was not demonstrated. orteca and yaba virus antisera both contained complement-fixing and neutralizing antibodies against swinepox virus, but swinepox antiserum had no ... | 1970 | 18605416 |
| a poxvirus of primates. i. growth of the virus in vitro and comparison with other poxviruses. | a primate poxvirus (orteca) isolated from a skin lesion in a rhesus monkey (macaca mulatta) was studied along with four known poxviruses: vaccinia, monkeypox, swinepox, and yaba. it grows in established lines of monkey kidney cells at a rate intermediate between that of vaccinia and monkeypox and that of swinepox and yaba. the cytopathic effects produced by orteca virus resemble those produced by swinepox virus, although, unlike swinepox, it cannot be propagated in porcine cells. neither can ort ... | 1970 | 18605415 |
| [swinepox--skin disease with sporadic occurrence]. | swinepox virus infection results in an acute, mild or subclinical course and is characterised by typical poxvirus skin lesions in affected pigs. additionally, sporadic vertical swinepox virus transmission leads to congenital generalised infection and subsequent abortion or stillbirth. the present report describes the occurrence of epidermal efflorescences in two piglets after intrauterine natural suipoxvirus infection. no clinical abnormalities of the gilt and littermates as well as in other pig ... | 2008 | 18500151 |
| variation in ligand binding specificities of a novel class of poxvirus-encoded tumor necrosis factor-binding protein. | the yatapoxviruses encode a distinct class of secreted tnf-binding protein (tnf-bp) that resembles an mhc class i heavy chain but distinct from any other known tnf inhibitor. characterization of these viral tnf inhibitors from tanapox virus, yaba monkey tumor virus (ymtv) and a closely related version from swinepox virus revealed dramatically differential tnf binding specificities for different mammalian species. the tanapox virus 2l protein (tpv-2l) formed inhibitory complexes with human tnf, a ... | 2006 | 16782702 |
| genome of deerpox virus. | deerpox virus (dpv), an uncharacterized and unclassified member of the poxviridae, has been isolated from north american free-ranging mule deer (odocoileus hemionus) exhibiting mucocutaneous disease. here we report the genomic sequence and comparative analysis of two pathogenic dpv isolates, w-848-83 (w83) and w-1170-84 (w84). the w83 and w84 genomes are 166 and 170 kbp, containing 169 and 170 putative genes, respectively. nucleotide identity between dpvs is 95% over the central 157 kbp. w83 and ... | 2005 | 15613325 |
| a secreted high-affinity inhibitor of human tnf from tanapox virus. | a class of secreted poxvirus tumor necrosis factor (tnf)-binding proteins has been isolated from tanapox-infected cell supernatants. the inhibitor bound to a tnf-affinity column and was identified as the product of the 2l gene. sequence analysis of 2l family members from other yatapoxviruses and swinepox virus yielded no sequence homology to any known cellular gene. the expressed tanapox virus 2l protein bound to human tnf with high affinity (k(d) = 43 pm) and exhibits an unusually slow off-rate ... | 2003 | 12676996 |
| c-mir, a human e3 ubiquitin ligase, is a functional homolog of herpesvirus proteins mir1 and mir2 and has similar activity. | kaposi's sarcoma associated-herpes virus encodes two proteins, mir (modulator of immune recognition) 1 and 2, which are involved in the evasion of host immunity. mir1 and 2 have been shown to function as an e3 ubiquitin ligase for immune recognition-related molecules (e.g. major histocompatibility complex class i, b7-2, and icam-1) through the bks (bovine herpesvirus 4, kaposi's sarcoma associated-herpes virus, and swinepox virus) subclass of plant homeodomain (phd) domain, termed the bks-phd do ... | 2003 | 12582153 |
| the genome of swinepox virus. | swinepox virus (swpv), the sole member of the suipoxvirus genus of the poxviridae, is the etiologic agent of a worldwide disease specific for swine. here we report the genomic sequence of swpv. the 146-kbp swpv genome consists of a central coding region bounded by identical 3.7-kbp inverted terminal repeats and contains 150 putative genes. comparison of swpv with chordopoxviruses reveals 146 conserved genes encoding proteins involved in basic replicative functions, viral virulence, host range, a ... | 2002 | 11752168 |
| construction of recombinant swinepox viruses and expression of the classical swine fever virus e2 protein. | to explore the swinepox virus (spv) as a potential live vector for immunization, a vector was developed for the construction of a recombinant spv carrying foreign genes. in this system, a foreign gene placed under the strong vaccinia virus promoter p(11) can be inserted into the viral thymidine kinase (tk) gene, and the recombinant virus can be isolated in a non-selective medium by the co-expression of e. coli lacz gene. compared with the wild type virus, the tk(-)recombinant spv showed a modest ... | 2001 | 11311343 |
| the genome sequence of yaba-like disease virus, a yatapoxvirus. | the genome sequence of yaba-like disease virus (yldv), an unclassified member of the yatapoxvirus genus, has been determined. excluding the terminal hairpin loops, the yldv genome is 144,575 bp in length and contains inverted terminal repeats (itrs) of 1883 bp. within 20 nucleotides of the termini, there is a sequence that is conserved in other poxviruses and is required for the resolution of concatemeric replicative dna intermediates. the nucleotide composition of the genome is 73% a+t, but the ... | 2001 | 11277691 |
| pseudosubstrate inhibition of protein kinase pkr by swine pox virus c8l gene product. | the interferon-induced protein kinase pkr is activated upon binding double-stranded rna and phosphorylates the translation initiation factor eif2alpha on ser-51 to inhibit protein synthesis in virally infected cells. swinepox virus c8l and vaccinia virus k3l gene products structurally resemble the amino-terminal third of eif2alpha. we demonstrate that the c8l protein, like the k3l protein, can reverse the toxic effects caused by high level expression of human pkr in yeast cells. in addition, exp ... | 2000 | 11040133 |
| swinepox virus as a vaccine vector for swine pathogens. | several small and large viruses (e.g., adenovirus, poxvirus, and herpesviruses) have been investigated as vaccine vectors. each viral system has its advantages and disadvantages. one major advantage for viral vector vaccines is their ability to elicit a protective cell-mediated immunity as well as a humoral response to the antigen delivered by the vector. one major problem to using recombinant viruses as vaccines is the pathogenic potential of the parent virus. therefore, it is important that al ... | 1999 | 9890036 |
| recombinant swinepox virus expressing beta-galactosidase: investigation of viral host range and gene expression levels in cell culture. | swinepox virus (spv) has been proposed as a potential vector for generating recombinant vaccines for swine. however, little is known about important aspects of spv biology, such as the functionality of spv promoters or the host range of spv. using a transient expression assay, well-characterized vaccinia virus promoters were shown to be active in cells infected with spv. a recombinant spv expressing beta-galactosidase (beta-gal) was constructed and characterized. the e. coli lacz gene was placed ... | 1998 | 9568038 |
| the t1/35kda family of poxvirus-secreted proteins bind chemokines and modulate leukocyte influx into virus-infected tissues. | immunomodulatory proteins encoded by the larger dna viruses interact with a wide spectrum of immune effector molecules that regulate the antiviral response in the infected host. here we show that certain poxviruses, including myxoma virus. shope fibroma virus, rabbitpox virus, vaccinia virus (strain lister), cowpox virus, and raccoonpox virus, express a new family of secreted proteins which interact with members of both the cc and cxc superfamilies of chemokines. however, swinepox virus and vacc ... | 1997 | 9123853 |
| identification and characterization of the thymidine kinase gene of yaba virus. | dna of yaba virus, a member of the poxviruses, was mapped by cross-hybridization between fragments of various restriction enzymes. the genome was approximately 135 kb in length and possessed two characteristic features of poxviruses: cross-links and inverted terminal repeats at both termini. hybridization of fragments of yaba virus dna to known vaccinia virus dna fragments indicated that the thymidine kinase (tk) gene mapped within the 0.9 kb xhoi-hincii fragment between 52.5 and 53.5 kb from th ... | 1995 | 7730796 |
| fowlpox virus as a vector in non-avian species. | examination of the members of the poxvirus family reveals a large and diverse group with members infecting almost every animal species. to a large extent, members of the individual genera have a broad host range and infect a number of animal species, although, as baxby has pointed out, successful experimental inoculation of a species does not necessarily mean that this species provides a natural host for that virus. avipox viruses, together with swinepox virus occupy a somewhat unique position i ... | 1988 | 2854335 |
| effects of cyclophosphamide on pigs infected and vaccinated with swinepox virus. | piglets treated with cyclophosphamide (50 mg/kg body wt, iv) before infection/vaccination with swinepox virus showed a strong suppression of humoral immune response. cell mediated immune response was also affected to some extent and the cyclophosphamide treated piglets did not resist the challenge on 21 day post infection/vaccination and developed mild lesions. | 1989 | 2557281 |
| wallace p. rowe lecture. poxviruses of laboratory animals. | currently the subfamily chordopoxvirinae, the poxviruses of vertebrates, is subdivided into eight genera, containing some 20-30 species; an inexact figure because the birdpox viruses have not yet been properly investigated taxonomically. i have discussed seven species belonging to three genera, all of which have caused infection and usually disease in mammals commonly used in the laboratory. the list could have been extended had i included chickens and swine as laboratory animals, for that would ... | 1990 | 2170745 |
| analysis of swinepox virus antigens using monoclonal antibodies. | seventeen monoclonal antibodies (mabs) against swinepox virus (spv) were produced and characterized. these mabs were classified into eight groups (a through h) on the basis of the molecular weight of the polypeptides which they recognized and the staining patterns of antigens in spv-infected cells by the indirect immunofluorescent (if) technique. the mabs belonging to groups a, b, c and g recognized late antigens in cytoplasmic inclusion bodies with molecular weights of 97 kd, 65 kd, 48 kd and 1 ... | 1992 | 1391185 |
| protection of guinea pigs by vaccination with a recombinant swinepox virus co-expressing ha1 genes of swine h1n1 and h3n2 influenza viruses. | swine influenza (si) is an acute respiratory infectious disease of swine caused by swine influenza virus (siv). siv is not only an important respiratory pathogen in pigs but also a potent threat to human health. here, we report the construction of a recombinant swinepox virus (rspv/h3-2a-h1) co-expressing hemagglutinin (ha1) of siv subtypes h1n1 and h3n2. immune responses and protection efficacy of the rspv/h3-2a-h1 were evaluated in guinea pigs. inoculation of rspv/h3-2a-h1 yielded neutralizing ... | 2013 | 23135159 |
| biological characterization and next-generation genome sequencing of the unclassified cotia virus span232 (poxviridae). | cotia virus (cotv) span232 was isolated in 1961 from sentinel mice at cotia field station, são paulo, brazil. attempts to classify cotv within a recognized genus of the poxviridae have generated contradictory findings. studies by different researchers suggested some similarity to myxoma virus and swinepox virus, whereas another investigation characterized cotv span232 as a vaccinia virus strain. because of the lack of consensus, we have conducted an independent biological and molecular character ... | 2012 | 22345477 |
| comparative analysis of poxvirus orthologues of the vaccinia virus e3 protein: modulation of protein kinase r activity, cytokine responses, and virus pathogenicity. | poxviruses are important human and animal pathogens that have evolved elaborate strategies for antagonizing host innate and adaptive immunity. the e3 protein of vaccinia virus, the prototypic member of the orthopoxviruses, functions as an inhibitor of innate immune signaling and is essential for vaccinia virus replication in vivo and in many human cell culture systems. however, the function of orthologues of e3 expressed by poxviruses of other genera with different host specificity remains large ... | 2011 | 21917954 |
| Swinepox virus outbreak, Brazil, 2011. | | 2011 | 22000394 |
| a novel vaccine against porcine circovirus type 2 (pcv2) and streptococcus equi ssp. zooepidemicus (sez) co-infection. | to develop a vaccine against porcine circovirus type 2 (pcv2) and streptococcus equi ssp. zooepidemicus (sez) co-infection, the genes of porcine il-18, capsid protein (cap) of pcv2 and m-like protein (szp) of sez were inserted into the swinepox virus (spv) genome by homologous recombination. the recombinant swinepox virus rspv-ics was verified by pcr and indirect immunofluorescence assays. to evaluate the immunogenicity of rspv-ics, 28 pcv2 and sez seronegative bama minipigs were immunized with ... | 2014 | 24726504 |
| construction and immunogenicity of a recombinant swinepox virus expressing a multi-epitope peptide for porcine reproductive and respiratory syndrome virus. | to characterize neutralizing mimotopes, phages were selected from a 12-mer phage display library using three anti-porcine reproductive and respiratory syndrome virus (prrsv) neutralizing monoclonal antibodies: (1) a1; (2) a2; and (3) a7. of these, a2 and a7 recognize the mimotope, p2, which contains the srhdhih motif, which has conserved consensus sequences from amino acid positions 156 to 161 in the n-terminal ectodomain of gp3. the artificial multi-epitope gene, mp2, was designed by combining ... | 2017 | 28272485 |
| c7l family of poxvirus host range genes inhibits antiviral activities induced by type i interferons and interferon regulatory factor 1. | vaccinia virus (vacv) k1l and c7l function equivalently in many mammalian cells to support vacv replication and antagonize antiviral activities induced by type i interferons (ifns). while k1l is limited to orthopoxviruses, genes that are homologous to c7l are found in diverse mammalian poxviruses. in this study, we showed that the c7l homologues from sheeppox virus and swinepox virus could rescue the replication defect of a vacv mutant deleted of both k1l and c7l (vk1l(-)c7l(-)). interestingly, ... | 2012 | 22345458 |
| immune responses and protection efficacy of a recombinant swinepox virus expressing ha1 against swine h3n2 influenza virus in mice and pigs. | swine influenza virus (siv) is not only an important respiratory pathogen in pigs but also a potent threat to human health. even though immunization with recombinant vaccinia poxviruses expressing protective antigens as a vaccination strategy has been widely used for many infectious diseases, development of recombinant swinepox virus (rspv) vector for this purpose has been less successful. here, we report the construction of a recombinant swinepox virus (rspv) expressing hemagglutinin (ha1) of h ... | 2012 | 22584406 |
| immune responses and protective efficacy of a recombinant swinepox virus expressing ha1 against swine h1n1 influenza virus in mice and pigs. | swine influenza virus (siv) is not only an important respiratory pathogen in pigs but also a potent threat to human health. although immunization with recombinant poxviruses expressing protective antigens as vaccines has been widely used for against many infectious diseases, development of recombinant swinepox virus (rspv) vector for the purpose has been less successful. here, we report the construction of a recombinant swinepox virus (rspv-ha1) expressing hemagglutinin (ha1) of h1n1 siv. immune ... | 2012 | 22391400 |
| first insights into the protective effects of a recombinant swinepox virus expressing truncated mrp of streptococcus suis type 2 in mice. | to explore the potential of the swinepox virus (spv) as vector for streptococcus suis vaccines, a vector system was developed for the construction of a recombinant spv carrying bacterial genes. using this system, a recombinant virus expressing truncated muramidase-released protein (mrp) of s. suis type 2 (ss2), designated rspv-mrp, was produced and identified by pcr, western blotting and immunofluorescence assays. the rspv-mrp was found to be only slightly attenuated in pk-15 cells, when compare ... | 2012 | 22515033 |
| recombinant swinepox virus expressing glycoprotein e2 of classical swine fever virus confers complete protection in pigs upon viral challenge. | classical swine fever (csf) is a highly contagious and serious viral disease that affects the pig industry worldwide. the glycoprotein e2 of the classical swine fever virus (csfv) can induce neutralizing antibodies, and it is widely used for novel vaccine development. to explore the development of a vaccine against csfv infections, the gene of glycoprotein e2 was inserted into the swinepox virus (spv) genome by homologous recombination. the culture titers of rspv-e2 remained at about 4.3 × 10(6) ... | 2017 | 28612010 |
| suppression of neointimal hyperplasia following angioplasty-induced vascular injury in pigs infected with swinepox virus. | many patients suffering from angina pectoris are treated with percutaneous coronary intervention (pci) and quickly develop angiographic renarrowing, or restenosis, at the site of pci treatment. restenosis is thought to arise from the combinatorial activation of thrombotic and inflammatory responses. the inflammatory response responsible for restenosis is also thought to involve the activation of a cascade of serine proteases and its subsequent regulation. poxviruses are known to possess a variet ... | 2012 | 23136622 |
| construction and immunogenicity of recombinant swinepox virus expressing capsid protein of pcv2. | to explore development of a vaccine against pcv2 infections, the gene of capsid protein (cap) was inserted into the swinepox virus (spv) genome by homologous recombination. the recombinant swinepox virus expressing capsid protein (rspv-cap) was verified by pcr, western blot and immunofluorescence assays. to evaluate the immunogenicity of rspv-cap, twenty-four pcv2 seronegative minipigs were immunized with rspv-cap, wild type spv (wtspv; negative control), or pbs (challenge control). after inocul ... | 2012 | 22884664 |
| corrigendum: construction and immunogenicity of a recombinant swinepox virus expressing a multi-epitope peptide for porcine reproductive and respiratory syndrome virus. | | 2017 | 28430207 |
| isolation and genetic characterization of swinepox virus from pigs in india. | swinepox virus (swpv), a member of the genus suipoxvirus causes generalized pock-like lesions on the body of domestic and wild pigs. although outbreak has been reported in india since 1987, virus isolation and genetic characterization remained elusive. in september 2013, an outbreak of acute skin infection occurred in piglets in a commercial piggery unit at rohtak district in haryana, india. the presence of swpv in scab samples collected from piglets succumbed to infection was confirmed by virus ... | 2016 | 27260812 |
| construction and immunogenicity of recombinant swinepox virus expressing outer membrane protein l of salmonella. | salmonella spp. are gram-negative flagellated bacteria that cause a variety of diseases in humans and animals, ranging from mild gastroenteritis to severe systemic infection. to explore development of a potent vaccine against salmonella infections, the gene encoding outer membrane protein l (ompl) was inserted into the swinepox virus (spv) genome by homologous recombination. pcr, western blot, and immunofluorescence assays were used to verify the recombinant swinepox virus rspv-ompl. the immune ... | 2016 | 27012234 |
| recombinant swinepox virus for veterinary vaccine development. | poxvirus-vectors have been widely used in vaccine development for several important human and animal diseases; some of these vaccines have been licensed and used extensively. swinepox virus (spv) is well suited to develop recombinant vaccines because of its large packaging capacity for recombinant dna, its host range specificity, and its ability to induce appropriate immune responses. | 2016 | 26458836 |
| efficacy and immunogenicity of recombinant swinepox virus expressing the a epitope of the tgev s protein. | to explore the possibility of developing a vaccine against transmissible gastroenteritis virus (tgev) infection, a recombinant swinepox virus (rspv-sa) expressing a tgev protective antigen has been constructed. immune responses and protection efficacy of the vaccination vector were assessed in both mice and pig models. an indirect elisa assay suggested that when mice were vaccinated with rspv-sa, the level of igg against tgev was enhanced dramatically. the cytokine assays were employed and the r ... | 2015 | 26116254 |