fingerprinting genomes by use of pcr with primers that encode protein motifs or contain sequences that regulate gene expression.pcr primers of arbitrary nucleotide sequence have identified dna polymorphisms useful for genetic mapping in a large variety of organisms. although technically very powerful, the use of arbitrary primers for genome mapping has the disadvantage of characterizing dna sequences of unknown function. thus, there is no reason to anticipate that dna fragments amplified by use of arbitrary primers will be enriched for either transcribed or promoter sequences that may be conserved in evolution. for these ...19921421760
an interspecific linkage map of mouse chromosome 15 positioned with respect to the centromere.we have used an interspecific backcross between c57bl/6j and mus spretus to derive a molecular genetic linkage map of chromosome 15 that includes 25 molecular markers and spans 93% of the estimated length of chromosome 15. using a second interspecific backcross that was analyzed with a centromere-specific marker, we were also able to position our map with respect to the chromosome 15 centromere. this map provides molecular access to many discrete regions on chromosome 15, thus providing a framew ...19921354638
analysis of sex-chromosome aneuploidy in interspecific backcross progeny between the laboratory mouse strain c57bl/6 and mus aneuploidy was identified in four female progeny of 200 interspecific backcrosses between laboratory mice (c57bl/6ros) and mus spretus. the progeny included two 39,xo monosomy mice resulting from a backcross with m. spretus, as well as a 41,xxx trisomic mouse and a 40,xx/41,xxx mosaic mouse resulting from two separate backcrosses with c57bl/6 mice. the parental origin and meiotic stage of the aneuploidies was determined for each of the mice using a series of markers that identifi ...19921349860
wobbler, a mutation affecting motoneuron survival and gonadal functions in the mouse, maps to proximal chromosome 11.the wobbler mouse (genotype wr/wr) has been considered as an animal model for human neurodegenerative disorders. in the homozygous condition, the autosomal mutation wobbler (wr) causes a motoneuron disease and gonadal dysfunction. we have genetically mapped the wr gene, using an interspecific backcross between the laboratory strain c57bl/6j (wr/+) and mus spretus. the expected percentage of wobbler progeny were obtained, but heterogeneous expression of the wobbler phenotype indicated the existen ...19921349581
[the existence in europe of four groups of mice (genus mus l.) of species and subspecies rank, demonstrated by biochemical genetics].analysis at 20 genetic loci by starch gel electrophoresis of a population from greece shows that contrary to most accepted views, the outdoor eastern mediterranean short-tailed mouse (i) does not belong to the same species as the house mouse (mus musculus), (ii) is not less distant from the eastern european semi-species of house mouse (biochemical group 2) than from the western and mediterranean european semi-species (group 1), and (iii) is not more closely related to the outdoor western mediter ...1978103641
[genetic analysis of interspecific crosses mus musculus l. x mus spretus lataste: linkage of adh-1 with amy-1 on chromosome 3 and es-14 with mod-1 on chromosome 9].192 offsprings from interspecific back-crosses (male m. spretus x female balb/c) f1 x male balb/c or (male m. spretus x female c57bl6) f1 x male c57bl6 were analysed at thirteen structural loci. linkage of es-14 with mod-1 on chromosome 9 and that of adh-1 with amy-1 on chromosome 3 are shown. the following order centromere/car-2/amy-1 is tentatively proposed for these loci on chromosome 3.197993520
[2 new esterase loci es-14 and es-15 in mice (genus muc l.) : characterization by different substrates and inhibitors].allelic differences at an esterase locus designated es-14 exist between mus musculus (both inbred strains and wild mice) and mus spretus. this locus is expressed in a variety of tissues. mus musculus has been assigned the allele es-14r while mus spretus carries the alternate alleles es-14l. each phenotype manifests itself as a single band of enzyme activity and f1 hybrids show two bands corresponding to the parental forms. allozyme variation was also detected at another esterase locus, temporari ...1979115606
the l-isoaspartyl/d-aspartyl protein methyltransferase gene (pcmt1) maps to human chromosome 6q22.3-6q24 and the syntenic region of mouse chromosome 10.we have mapped the genes for the human and mouse l-isoaspartyl/d-aspartyl protein carboxyl methyltransferase (ec using cdna probes. we determined that the human gene is present in chromosome 6 by southern blot analysis of dna from a panel of mouse-human somatic cell hybrids. in situ hybridization studies allowed us to confirm this identification and further localize the human gene (pcmt1) to the 6q22.3-6q24 region. by analyzing the presence of an ecori polymorphism in dna from backcros ...19921478665
localization of growth arrest-specific genes on mouse chromosomes 1, 7, 8, 11, 13, and 16.growth arrest in nih3t3 cells is associated with increased expression of a variety of mrnas, several of which have been isolated as cdna clones. six of these growth arrest-specific (gas) genes were mapped by following the inheritance of dna restriction fragment length variants (rflvs) associated with them in panels of recombinant inbred (ri) strains of mice and in the progeny of backcrosses both between laboratory mouse strains and between a laboratory strain and mus spretus. the six genes are u ...19921347472
activation of the prolactin receptor gene by promoter insertion in a moloney murine leukemia virus-induced rat thymoma.the prolactin receptor (prlr) and growth hormone receptor (ghr) genes and the moloney murine leukemia virus integration-2 (mlvi-2) locus were mapped to mouse chromosome 15 and human chromosome 5 bands p12-p14. to examine the potential relationship between mlvi-2 and the genes encoding the growth hormone receptor and the prolactin receptor, we determined the chromosomal location of all three loci in the rat, using a panel of rat-mouse somatic cell hybrids, and in the mouse, using a panel of (c57b ...19921404614
the mouse alpha 1(xii) and human alpha 1(xii)-like collagen genes are localized on mouse chromosome 9 and human chromosome 6.type xii collagen is a member of the facit (fibril-associated collagens with interrupted triple helices) group of extracellular matrix proteins. like the other members of this group, collagen types ix and xiv, type xii has alternating triple-helical and non-triple-helical domains. because of its structure, its association with collagen fibrils, and its distribution in dense connective tissues, type xii is thought possibly to act as a cross-bridge between fibrils and resist shear forces caused by ...19921427837
deficiency of x and y chromosomal pairing at meiotic prophase in spermatocytes of sterile interspecific hybrids between laboratory mice (mus domesticus) and mus spretus.the normal association between the x and y chromosomes at metaphase i of meiosis, as seen in air-dried light microscope preparations of mouse spermatocytes, is frequently lacking in the spermatocytes of the sterile interspecific hybrid between the laboratory mouse strains c57bl/6 and mus spretus. the purpose of this work is to determine whether the separate x and y chromosomes in the hybrid are asynaptic, caused by failure to pair, or desynaptic, caused by precocious dissociation. unpaired x-y c ...19921424992
a linkage map of mouse chromosome 1 using an interspecific cross segregating for the gld autoimmunity interspecific backcross was used to define a high resolution linkage map of mouse chromosome (chr) 1 and to analyze the segregation of the generalized lymphoproliferative disease (gld) mutation. mice homozygous for gld have multiple features of autoimmune disease. analysis of up to 428 progeny from the backcross [(c3h/hej-gld x mus spretus)f1 x c3h/hej-gld] established a map that spans 77.6 cm and includes 56 markers distributed over 34 ordered genetic loci. the gld mutation was mapped to a l ...19921543910
mapping of the structural gene for s-adenosyl homocysteine hydrolase to mouse chromosome 2, and related sequences to chromosomes 8 and x.comparative mapping studies in human and mouse have shown that, to date, human chromosome (chr) 20 is completely syntenic with distal mouse chr 2. the structural locus for s-adenosyl-l-homocysteine hydrolase (ec in human, ahcy, maps to 20 qter-->q13.1, and we report here that the homologous locus in the mouse, ahcy, maps to distal mouse chr 2 with gene order pcna-ahcy-ada. analysis of 123 progeny of an interspecific backcross between a laboratory stock, an, and mus spretus using a rat c ...19921450513
analysis of high complement levels in mus hortulanus and bub mice.bub/bnj mice were previously identified as having exceptionally potent complement activity, relative to common mouse strains, in the lysis of antibody-coated human tumor cells. we describe herein our investigation into the molecular and genetic basis for this difference between mouse strains, and also our results with wild mice and mouse strains recently derived from the wild, to determine whether low complement levels are characteristic of wild mice. bub complement was compared with complement ...19921401942
genetic linkage analysis of the murine developmental mutant velvet coat (ve) and the distal chromosome 15 developmental genes hox-3.1, rar-g, wnt-1, and krt-2.we have identified restriction fragment length polymorphisms between mus musculus and mus spretus for the chromosome 15 loci hox-3, wnt-1, krt-2, rar-g, and ly-6. we followed the inheritance of these alleles in interspecific genetic test crosses between velvet coat (ve) heterozygotes and m. spretus. the results suggest a gene order and recombination distances (in cm) of ly-6-22-wnt-1-2-ve/krt-2/rar-g-3-hox-3. no recombination was found between ve, krt-2, and rar-g. the data also provide evidence ...19921379621
mouse microsatellites from a flow-sorted 4:6 robertsonian chromosome.twenty microsatellites were generated from a previously characterized lambda gt10 library containing c57bl/6j mouse dna from a flow-sorted 4:6 robertsonian chromosome. these sequences were analyzed for size variation between different strains of mice with the polymerase chain reaction (pcr) and mapped by use of either strain distribution patterns (sdps) in recombinant inbred (ri) strains, or intra- and interspecific backcrosses. eighty-five percent of the sequences showed allelic variations betw ...19921450512
a molecular genetic linkage map of mouse chromosome 18 reveals extensive linkage conservation with human chromosomes 5 and interspecific backcross between c57bl/6j and mus spretus was used to generate a molecular genetic linkage map of mouse chromosome 18 that includes 23 molecular markers and spans approximately 86% of the estimated length of the chromosome. the apc, camk2a, d18fcr1, d18fcr2, d18leh1, d18leh2, dcc, emb-rs3, fgfa, fim-2/csfmr, gnal, grl-1, grp, hk-1rs1, ii, kns, lmnb, mbp, mcc, mtv-38, palb, pdgfrb, and tpl-2 genes were mapped relative to each other in one interspecific backcross. a second inters ...19921354644
a multipoint genetic linkage map of mouse chromosome 18.we have mapped 13 loci on mouse chromosome 18 by southern blot analysis of restriction fragment length polymorphisms among progeny from an interspecific backcross: (c57bl/6j x mus spretus) x m. spretus. complete haplotype analysis of 136 of these progeny was used to establish gene order and estimate genetic distances between loci. the gene order (from centromere to telomere) and recombination distances (in centimorgans) were as follows: pgk-1rs5-4.3-tpi-10-11.8-(egr-1, hmg17-rs9)-2.1-fgfa-2.2-gr ...19921354640
isolation and initial characterization of a large repeat sequence element specific to mouse chromosome 8.a clone containing 15.6 kb of mouse genomic dna was specifically localized to murine chromosome 8 by fluorescence in situ hybridization. the major signal, mapping just below the centromeric heterochromatin, was much too intense for a single-copy probe. two additional weak hybridization signals were detected in or near distal bands 8b3 and 8d. six subclones spanning the entire 15.6-kb insert gave strong centromere proximal signals; however, none of these clones cross-hybridized with each other, s ...19921559702
identification and mapping of six microdissected genomic dna probes to the proximal region of mouse chromosome 1.six independent dna probes, lambda mm1c-150, lambda mm1c-153, lambda mm1c-156, lambda mm1c-162, lambda mm1c-163, and lambda mm1c-165, have been isolated from a library of microdissected fragments from mouse chromosome 1, spanning cytogenetic bands c2 to c5. these dna probes have been mapped by restriction fragment length polymorphism analysis with respect to 12 marker loci previously assigned to this portion of mouse chromosome 1, in a panel of 251 segregating mus spretus x c57bl/6j interspecifi ...19921358796
genetic mapping of two dna markers, d16ros1 and d16ros2, flanking the mutation site in the chakragati mouse, a transgenic insertional mutant.we present here the genetic mapping of two novel loci, d16ros1 and d16ros2, to mouse chromosome (chr) 16. the probes for these loci were genomic fragments isolated from the chakragati mouse, a behavioral mutant resulting from insertional mutagenesis during the course of making transgenic mice. d16ros1 and d16ros2 were first mapped by recombinant inbred (ri) strain analysis and subsequently by the analysis of 145 progeny of two interspecific backcrosses between mus domesticus and mus spretus. the ...19921581634
chromosomal localization of the murine gene and two related sequences encoding high-mobility-group i and y proteins.hmg-i and its isoform hmg-y are members of the abundant high-mobility-group of nonhistone chromatin proteins; they bind to a + t-rich regions of chromosomal dna and are expressed at high levels in rapidly dividing, undifferentiated mammalian cells. hmg-i and hmg-y are alternatively spliced products of a single functional gene, designated hmgi in the mouse. here, we report the occurrence of at least three distinct hmgi-related loci in the mouse. only one of these loci was present in all of the 10 ...19921559701
mapping of fmr1, the gene implicated in fragile x-linked mental retardation, on the mouse x chromosome.a genetic map of the cf-9 to dmd region of the mouse x chromosome has been established by typing 100 offspring from a mus musculus x mus spretus interspecific backcross for the four loci cf-9, cdr, gabra3, and dmd. the following order and genetic distances in centimorgans were determined: (cf-9)-2.4 +/- 1.7-(cdr)-2.0 +/- 1.4-(gabra3)-4.1 +/- 2.0-(dmd). six backcross offspring carrying x chromosomes with recombination events in the cdr-dmd region were identified. these recombination events were u ...19921572655
genetic mapping on the mouse x chromosome of human cdna clones for the fragile x and hunter syndromes.murine x-linked genes corresponding to the human fragile x (fmr1) and hunter syndrome (ids) loci have been mapped in an interspecific backcross between b6cba-aw-j/a-bpa and mus spretus using human cdna clones. pedigree analysis of recombinants from a total of 248 backcross progeny favors a gene order of (cf-9, mcf-2)-(fmr-1)-ids-gabra3-rsvp. gene order is conserved between the species, although no fragile site has been detected in the mouse in this region of the murine x chromosome.19921572654
lactate dehydrogenase polymorphism in mus musculus l. and mus spretus lataste.first variation at the ldh-a locus and a new allele at the ldh-b locus are reported in a m. musculus population dimorphic at the ldh-a locus and in a m. spretus population trimorphic at the ldh-b locus.1978720506
[hybridization between mus musculus l. and mus spretus lataste under laboratory conditions (author's transl)].2 species of mice mus musculus brevirostris and mus spretus are sympatric in southern france and reproductively isolated. however, under laboratory conditions, they breed and give fully viable f 1 hybrids. this indicates that premating isolating mechanisms probably occur in nature and have yet to be investigated. moreover, it now enables us to introduce new genetical markers in laboratory strains.1978720505
evidence for the presence of two sympatric species of mice (genus mus l.) in southern france based on biochemical genetics.populations of mice established outdoors as well as indoors have been investigated at 24 loci using starch gel electrophoresis. two reproductively isolated groups are recognized, one of which is referable to a house mouse subspecies, mus musculus brevirostris, and the other to a different species. mus spretus, contrary to the view of schwarz and schwarz that only one species of mus is present in the mediterranean basin. the genetic distance between these two groups is larger than between any pai ...1978678291
genetic mapping of the mouse x chromosome in the region homologous to human xq27-xq28.the four loci gabra3, dxpas8, caml1, and bpa, located near the murine x-linked visual pigment gene (rsvp), have been ordered using 248 backcross progeny from an interspecific mating of (b6cba-aw-j/a-bpa) and mus spretus. one hundred twenty backcross progeny have been analyzed at seven anchor loci spanning the x chromosome and form a regional mapping panel. an additional 128 progeny have been screened for recombination events between cf-9 and dmd. eighteen recombinants between these loci have bee ...19911674728
linkage, but not gene order, of homologous loci, including alpha-l-iduronidase (idua), is conserved in the huntington disease region of the mouse and human genomes.alpha-l-iduronidase (idua), which when deficient causes mucopolysaccharidosis type i, is located near the huntington disease locus (hd) on human chromosome (chr) 4p16.3, approximately 10(6) base pairs (bp) from the telomere. as part of our continuing efforts to define a detailed comparative map for this chromosomal segment in mice and humans, we have used an interspecific backcross between c57bl/6j and an inbred strain derived from mus spretus to map idua, the mouse homolog of idua. we also mapp ...19921533802
mouse ferritin h multigene family is polymorphic and contains a single multiallelic functional gene located on chromosome 19.multiple ferritin h subunit sequences are present in the genome of higher vertebrates, but it is not yet known with certainty if more than one is expressed. in this paper, we provide evidence that there is only one functional ferritin h gene in the mouse. we screened a mouse genomic library using a mouse ferritin h cdna as a probe and characterized five clones. these genomic clones proved to contain three pseudogenes and two allelic forms of a unique functional gene. these two alleles differed b ...19911679743
mapping of the glycine receptor alpha 2-subunit gene and the gabaa alpha 3-subunit gene on the mouse x chromosome.we have mapped the gene for the alpha 2-subunit of the inhibitory glycine receptor (glra2) to the telomeric end of the mouse x chromosome by backcross analysis of a mus musculus/mus spretus interspecific cross. in addition, we have extended the mapping of the gabaa alpha 3-subunit receptor gene (gabra3). a deduced gene order of cen-cybb-hprt-dxpas6-gabra3-rsvp-gdx/cf-8- dmd-pgk-1-dxpas2-plp-dxpas1-glra2-tel places gabra3 proximal to the visual pigment gene rsvp and glra2 in the region of loci fo ...19911679744
molecular mapping of mouse chromosomes 4 and 6: use of a flow-sorted robertsonian chromosome.the development of dense genetic maps of mammalian chromosomes is facilitated when chromosome-specific libraries are used as a source of genetic markers. to saturate the genetic maps of mouse chromosomes 4 and 6, we have made use of fluorescent-activated chromosome sorting to purify a 4:6 robertsonian chromosome from a cell line harboring the rb(4:6)2bnr translocation. after staining with chromomycin a3 and hoechst 33528, this chromosome was separated from the other mouse chromosomes. dna was is ...19921639403
three brain sodium channel alpha-subunit genes are clustered on the proximal segment of mouse chromosome 2.we have used long-range physical mapping and restriction fragment length polymorphisms between two mouse species to determine the chromosomal organization and location of the genes encoding three distinct isoforms of the alpha-subunit of the brain sodium channel. physical mapping by pulsed-field gel electrophoresis has established that scn2a and scn3a (genes encoding type ii and type iii sodium channel alpha-subunit isoforms) are physically linked and are separated by a maximum distance of 600 k ...19911679748
genetic variation in mouse apolipoprotein a-iv due to insertion and deletion in a region of tandem repeats.we have detected three unique apolipoprotein a-iv (apoa-iv) charge isoforms in strains of commensal mice. the cdna sequences for one representative of each isoform (mus domestesticus strains c57bl/6j and 129/j and mus castaneus) revealed a polymorphism within a series of four imperfect repeats encoding the sequence glu-gln-ala/val-gln. insertions or deletions of 12 nucleotides within this repetitive region have given rise to three genotypes characterized by three (129), four (c57bl/6), or five ( ...19911648102
high-density molecular map of the central span of the mouse x chromosome.a total of 17 linking clones previously sublocalized to the central span of the mouse x chromosome have been ordered by detailed analysis through interspecific mus spretus/mus musculus domesticus backcross progeny. these probes have been positioned with respect to existing dna markers utilizing a new interspecific backcross segregating for the tabby (ta) locus. the density of clones within this 11.5-cm interval is now, on average, one clone every 1000 kb. this high-density map provides probes in ...19911675191
determination of a molecular map position for hyp using a new interspecific backcross produced by in vitro fertilization.we have established a mus spretus/mus musculus domesticus interspecific backcross segregating for two x-linked mutant genes, ta and hyp, using in vitro fertilization. the haplotype of the recombinant x chromosome of each of 241 backcross progeny has been established using the x-linked anchor loci otc, hprt, dmd, pgk-1, and amg and the additional probes dxsmh43 and cbx-rs1. the hyp locus (putative homologue of the human disease gene hypophosphatemic rickets, hyp) has been incorporated into the mo ...19911685478
homologous chromosomal locations of the four genes for inter-alpha-inhibitor and pre-alpha-inhibitor family in human and mouse: assignment of the ancestral gene for the lipocalin superfamily.the inter-alpha-inhibitor (i alpha i) and pre-alpha-inhibitor (p alpha i) family is composed of three plasma protease inhibitors, i alpha i, p alpha i, and bikunin, whose chains are encoded by a set of three evolutionarily related heavy (h) chain genes designated h1, h2, and h3 and a fourth gene, the so-called alpha 1-microglobulin/bikunin precursor (ambp) gene. the latter codes for a precursor that splits into: (i) alpha 1-microglobulin, which belongs to the lipocalin superfamily; and (ii) biku ...19921385302
molecular genetic linkage maps of mouse chromosomes 4 and 6.we have generated a moderate resolution genetic map of mouse chromosomes 4 and 6 utilizing a (c57bl/6j x mus spretus) f1 x mus spretus backcross with rflps for 31 probes. the map for chromosome 4 covers 77 cm and details a large region of homology to human chromosome 1p. the map establishes the breakpoints in the mouse 4-human 1p region of homology to a 2-cm interval between ifa and jun in mouse and to the interval between jun and acadm in human. the map for mouse chromosome 6 spans a 65-cm regi ...19911684952
mapping anti-müllerian hormone (amh) and related sequences in the mouse: identification of a new region of homology between mmu10 and hsa19p.a panel of 78 backcross progeny, balb/cj x (balb/cj x cast/ei)f1, was used to map the gene encoding anti-müllerian hormone (amh), also called müllerian inhibiting substance, to mouse chromosome 10 (mmu10). this analysis identified a new region of linkage homology between human chromosome 19p (hsa 19p) and mmu10 and localized an apparent recombinational hot spot in (c57bl/6j x mus spretus)f1 females [compared with (balb/cj x cast/ei)f1 males] to the interval between phenylalanine hydroxylase (pah ...19911685136
molecular mapping of the mouse ob mutation.the mouse ob mutation has been mapped relative to a series of rflps among the progeny of three separate mouse crosses: an intraspecific backcross, an intraspecific intercross, and an interspecific intercross. genotypic assignment at the ob locus was made by making use of measurements of body mass index and the plasma concentrations of glucose and insulin. these data have suggested that the development of diabetes in these animals is a consequence of unlinked polygenes. there was also evidence th ...19911686014
recent evolutionary origin of the expression of the glial fibrillary acidic protein (gfap) in lens epithelial cells. a molecular and genetic analysis of various mouse species.we have investigated the phylogenetic distribution of the glial fibrillary acidic protein (gfap) in lens epithelial cells (lec) of various mouse species within the genus mus. we have shown that lens gfap is expressed in mice of the mus musculus complex and in mus spicilegus and mus macedonicus species (l.gfap(+) phenotype) while it is absent in mus spretus, mus caroli and mus cooki species (l.gfap(-) phenotype). our results argue in favour of one of the phenograms illustrating the probable phylo ...19911712888
an amino acid change in the dna-binding region of sry, found in mus musculus domesticus and other species, does not explain c57bl/6j-ydom sex-reversal.sry is the testes determining factor gene located on the y chromosome. we have performed dideoxy sequencing or restriction enzyme digestion of sry sequences amplified by pcr from a variety of inbred strains and species of mice. a nonconservative replacement of thr for isoleu at position 61 was found in sry from the y chromosome of mus musculus domesticus, mus spretus, and mus caroli. we demonstrate that this variation is not sufficient to explain the gradiation in the sex-reversal seen when diff ...19921599466
rapid physical mapping of cloned dna on banded mouse chromosomes by fluorescence in situ hybridization.physical mapping of dna clones by nonisotopic in situ hybridization has greatly facilitated the human genome mapping effort. here we combine a variety of in situ hybridization techniques that make the physical mapping of dna clones to mouse chromosomes much easier. hybridization of probes containing the mouse long interspersed repetitive element to metaphase chromosomes produces a giemsa-like banding pattern which can be used to identify individual mus musculus, mus spretus, and mus castaneus ch ...19921733847
mouse chromosome-specific markers generated by pcr and their mapping through interspecific backcrosses.we have utilized an oligonucleotide primer from the 3' end of the mouse l1 repeat element for amplification of mouse-specific inter-repeat pcr products from chinese hamster/mouse somatic cell hybrids. pcr of a chinese hamster/mouse somatic cell hybrid (96az2), containing only mouse chromosome 16, produced a range of mouse-specific bands. two of the mouse-specific pcr products, of 250 and 580 bp, have been confirmed as originating from mouse chromosome 16 by somatic cell hybrid analysis. both the ...19911774069
pcr-analyzed microsatellites: data concerning laboratory and wild-derived mouse inbred strains.we have investigated 67 primers designed by dr. j. todd and co-workers to amplify microsatellites sequences in the mouse. we report on additional polymorphisms concerning seven laboratory inbred strains, complementary to those already published. we include the survey of three independently derived strains of mus spretus: spe/pas, seg/pas and spr/smh. spe/pas and seg/pas are very close (3% polymorphism), whereas the third one, (spr/smh), is very different from the other two strains (33% polymorph ...19911794054
additional microsatellite markers for mouse genome mapping.mouse sequence information from the embl and genbank databases, published sequences and genomic clones have been analyzed for simple repetitive elements or microsatellites. each microsatellite has been amplified by the polymerase chain reaction (pcr) as a single locus marker. pcr primers were designed from unique sequence flanking each repeat. size variation of pcr products less than 750 base pairs (bp) between mouse strains has been determined using ethidium bromide-stained acrylamide or agaros ...19911794058
polymorphisms revealed by pcr with single, short-sized, arbitrary primers are reliable markers for mouse and rat gene mapping.ten single, arbitrarily designed oligodeoxynucleotide primers, with 50-70% (g+c) content, were used to amplify by polymerase chain reaction (pcr) sequences with dna templates from several mouse species (mus spretus, mus musculus musculus, and mus musculus domesticus), as well as dna from the laboratory rat (rattus norvegicus). eight of these ten primers, used either individually or associated in pairs, generated a total of 13 polymorphic products which were used as genetic markers. all of these ...19921617216
genomic mapping within the albino-deletion complex using individual early postimplantation mouse embryos.sensitive methods for analysis of dna from limited amounts of tissue are often difficult, error prone, and time consuming. here, we describe a procedure for molecular analysis of individual early postimplantation mouse embryos by southern analysis. the procedure involves embedding single embryos in agarose before lysing and deproteinizing in situ. the embedded dna can be digested with restriction enzymes and analyzed by standard southern-blotting procedures. the procedure is sensitive enough to ...19921617218
mapping and expression of the ubiquitin-activating enzyme e1 (ube1) gene in the mouse.the nucleotide sequence of the human cdna encoding ubiquitin-activating enzyme e1 is more than 99% identical with the human a1s9t cdna, a gene that has been shown to complement the temperature-sensitive mutant mouse cell line, tsa1s9. the amino acid sequences of the proteins encoded by these two cdna sequences are identical, and both cdnas were previously shown to be located in the same region of the human x chromosome; thus, ubiquitin-activating enzyme e1 and a1s9t appear to be the same gene, d ...19921617221
in situ analysis of centromeric satellite dna segregating in mus species situ hybridization of biotin-labeled mouse major satellite dna clone pmr196 was applied to mus domesticus and mus spretus chromosomes (chr). the same karyotypes were counterstained with distamycin a-dapi to identify at-rich heterochromatin. chromosomes from the laboratory mouse, c57bl/6ros (bl/6; m. domesticus) were uniformly labeled at the centromere except for the y, while chromosomes from the divergent mus species m. spretus showed little or no hybridization. differences between mus specie ...19911799790
the multipoint genetic mapping of mouse chromosome 16.utilizing a mus spretus/mus domesticus (c57bl/10) interspecific backcross, we have constructed a multipoint genetic map of mouse chromosome 16 that extends 43.2 cm from the proximal prm-1 locus to the distal ets-2 locus. the genetic map incorporates three new markers: d16smh6, a random genomic clone; pgk-1ps1, a phosphoglycerate kinase pseudogene; and the growth-associated protein gap43. the map position of gap43 indicates the presence, on mouse chromosome 16, of a significant-size conserved lin ...19911672298
interspecific backcross mice show sex-specific differences in allelic inheritance.transmission distortion is identified as a difference in transmission frequency of two alleles from the normal 1:1 mendelian segregation in diploid organisms. transmission distortion can extend over part or all of a chromosome. the recent development of interspecific mouse backcrosses has provided a powerful method for multilocus mapping of entire chromosomes in a single cross, and consequently for identifying distortions in allelic inheritance. we used an interspecific backcross of [(c57bl/6j x ...19911916246
the properdin structural locus (pfc) lies close to the locus for tissue inhibitor of metallothionine proteases (timp) on the mouse x chromosome.we have confirmed the assignment of the structural locus of the complement factor properdin (pfc) to the mouse x chromosome and mapped it between monoamine oxidase-a (mao-a) and hypoxanthine phosphoribosyltransferase (hprt) using a mus spretus x mus musculus interspecific backcross of 108 animals. the structural locus for murine tissue inhibitor of metallothionine proteases (timp) could not be separated from properdin in a panel of 18 recombinant animals. by minimizing the number of double recom ...19911916808
the generation of a library of pcr-analyzed microsatellite variants for genetic mapping of the mouse genome.forty-three sequences containing simple sequence repeats or microsatellites were generated from an m13 library of total genomic mouse dna. these sequences were analyzed for size variation using the polymerase chain reaction and gel electrophoresis without the need for radiolabeling. seventy-two percent of the sequences showed allelic size variations between different inbred strains of mouse and the wild mouse, mus spretus; and 53% showed variation between inbred strains. thirty-seven percent wer ...19911916820
molecular genetic analysis of the ta25h deletion: evidence for additional deleted loci.seventeen linking clones sublocalized to the central region of the mouse x chromosome (chr) were screened against genomic dna from male mice carrying the tabby-25h (ta25h) deletion. two of these linking clones, lambda em131 and lambda em169, were found to be deleted in ta25h/y animals. genetic mapping through mus musculus domesticus/mus spretus interspecific backcross progeny, segregating for the original tabby (ta) gene mutation, was utilized to order these markers and to define nearest flankin ...19911797229
genetic maps of mouse chromosome 17 including 12 new anonymous dna loci and 25 anchor interspecific backcross between lab mice and mus spretus was used to construct a multilocus map of chromosome 17 consisting of 12 new anonymous loci and 9 anchor loci. in addition, 7 anonymous dna loci were added to the chr 17 map for the bxd strains. although we were able to identify readily the most likely gene order in the interspecific backcross, we found no evidence for an unambiguous gene order using the bxd recombinant inbred strains. comparison of the interspecific backcross map and t ...19911672293
close linkage of the murine locus bare patches to the x-linked visual pigment gene: implications for mapping human x-linked dominant chondrodysplasia punctata.the murine x-linked dominant mutation bare patches (bpa) has a phenotype similar to and is likely homologous to human x-linked dominant chondrodysplasia punctata (cdpx2). classic two-point linkage analysis in the mouse with distant markers suggested that bpa maps near glucose-6-phosphate dehydrogenase (g6pd). we have confirmed the regional localization using interspecific matings with mus spretus. we have also detected a restriction fragment length polymorphism (rflp) at the murine x-linked visu ...19901973136
a novel mouse chromosome 17 hybrid sterility locus: implications for the origin of t haplotypes.the effects of heterospecific combinations of mouse chromosome 17 on male fertility and transmission ratio were investigated through a series of breeding studies. animals were bred to carry complete chromosome 17 homologs, or portions thereof, from three different sources-mus domesticus, mus spretus and t haplotypes. these chromosome 17 combinations were analyzed for fertility within the context of a m. domesticus or m. spretus genetic background. two new forms of hybrid sterility were identifie ...19911936961
establishment of the mouse chromosome 7 region with homology to the myotonic dystrophy region of human chromosome 19q.a number of genetic markers, including atp1a3, tgfb, ckmm, and prkcg, define the genetic region on human chromosome 19 containing the myotonic dystrophy locus. these and a number of other dna probes have been mapped to mouse chromosome 7 utilizing a mouse mus domesticus/mus spretus interspecific backcross segregating for the genetic markers pink-eye dilution (p) and chinchilla (cch). the establishment of a highly syntenic group conserved between mouse chromosome 7 and human chromosome 19q indica ...19901970795
fine mapping of a putative rd cdna and its co-segregation with rd expression.retinal degeneration is inherited in an autosomal recessive pattern in the retinal degeneration (rd) mouse. the defective gene for this disease has been mapped to mouse chromosome 5 between the well-defined (anchor) genes afp and gus. we recently cloned a putative rd cdna, zr.408, using a strategy based on subtractive and differential hybridization. zr.408 was shown to hybridize to a larger message from rd/rd mice than from normal mice in northern blots; was mapped to mouse chromosome 5; and was ...19901974892
molecular mapping of the mouse db mutation.diabetes (db) is an autosomal recessive mutation located in the midportion of mouse chromosome 4 that results in profound obesity with hyperphagia, increased metabolic efficiency, and insulin resistance. to clone this gene and generate a molecular map of the region around this mutation, two genetic crosses were established: an intraspecific backcross between c57bl/6j db/db females and c57bl/6j db/db x dba/2j +/+ f1 (b6d2 db/+ f1) male mice and an interspecific intercross between b6d2 db/+ f1 mal ...19901978328
chromosomal location of the octamer transcription factors, otf-1, otf-2, and otf-3, defines multiple otf-3-related sequences dispersed in the mouse genome.chromosomal locations have been assigned for the octamer transcription factor, otf, gene family (previously named the octamer-binding protein, oct, gene family) using an interspecific backcross of [(c57bl/6j x mus spretus)f1 x c57bl/6j] mice and the bxh recombinant inbred strains. molecular probes for otf-1 and otf-2 recognized single loci on mouse chromosomes 1 and 7, respectively, whereas probes for otf-3 recognized a minimum of eight independently segregating loci (designated otf-3a through o ...19911676977
characterization of the murine thrombospondin gene.thrombospondin is an adhesive glycoprotein that supports cell attachment, spreading, and migration. the murine thrombospondin gene is approximately 18 kb in length and includes 22 exons. interspecific backcross analysis using progeny derived from matings of (c57bl/6j x mus spretus) f1 x c57bl/6j mice indicates that the thrombospondin gene is tightly linked to the fshb, actcl, ltk, and b2m loci on murine chromosome 2. the sequence of the murine gene is very similar to that of the human gene in (1 ...19911774063
zfa is an expressed retroposon derived from an alternative transcript of the zfx gene.zfy, a gene on the y chromosome encoding a zinc finger protein, has been proposed as a candidate for the human testis determining gene. sequences related to zfy, called zfx, are present on the x chromosome of a wide range of placental mammals. unlike most mammals the mouse has four genes homologous to zfy; two on the y chromosome, zfy-1 and zfy-2, an x-linked gene, zfx, and an autosomal gene, zfa. we show here that zfa has arisen recently by retroposition of one of at least three alternatively s ...19901691708
detailed genetic mapping of the a-raf proto-oncogene on the mouse x chromosome.the transcribed murine a-raf proto-oncogene has been localized to the proximal region of the mouse x chromosome, within the context of four other active genes in this region which together constitute a conserved linkage group between mouse and man. this localization has been accomplished using species-specific restriction fragment length variation and dnas from a previously defined informative subset of progeny representative of a set of 100 progeny from an interspecific backcross between inbred ...19912011396
linkage analysis of the murine hyal-1 locus on chromosome 9.we have recently described a new locus, hyal-1, which determines hyaluronidase variants in mouse serum. on the basis of segregation in recombinant inbred and congenic strains, hyal-1 was tentatively assigned to chromosome 9 (fiszer-szafarz and de maeyer, '89). in the present study we have performed a linkage analysis of hyal-1 using 156 backcross progeny of an interspecies cross of laboratory mice and mus spretus. linkage was tested to two anchor loci on chromosome 9: d (dilute, a coat color loc ...19912022949
conservation of position and exclusive expression of mouse xist from the inactive x chromosome.x-chromosome inactivation in mammals is a regulatory phenomenon whereby one of the two x chromosomes in female cells is genetically inactivated, resulting in dosage compensation for x-linked genes between males and females. in both man and mouse, x-chromosome inactivation is thought to proceed from a single cis-acting switch region or inactivation centre (xic/xic). in the human, xic has been mapped to band xq13 (ref. 6) and in the mouse to band xd (ref. 7), and comparative mapping has shown that ...19912034279
loss of heterozygosity and mutational alterations of the p53 gene in skin tumours of interspecific hybrid mice.functional alterations or loss of tumor-suppressor genes are an important feature of neoplastic progression in humans. the employment of suitable animal model systems would greatly facilitate the detection and manipulation of such genes. we describe here an experimental approach to this problem based on the analysis of skin tumors induced in f1 hybrids between mus musculus and mus spretus mice. the results show that loss of heterozygosity on chromosome 11 occurred in 4/13 mouse skin carcinomas, ...19911766680
systematic identification of line-1 repetitive dna sequence differences having species specificity between mus spretus and mus domesticus.line-1 is a family of repetitive dna sequences interspersed among mammalian genes. in the mouse haploid genome there are about 100,000 line-1 copies. we asked if the subspecies mus spretus and mus domesticus have developed species-specific line-1 subfamilies. sequences from 14 m. spretus line-1 elements were obtained and compared to m. domesticus line-1 sequences. using a molecular phylogenetic tree we identified several differences shared among a subset of young repeats in one or the other spec ...19912056531
genetic mapping in the region of the mouse x-inactivation center.the mouse x-inactivation center lies just distal to the t16h breakpoint. utilizing pedigree analysis of backcross progeny from a mus domesticus/mus spretus interspecific cross, we have mapped a number of genetic loci, gene probes, microclones, and eagi linking clones distal to the t16h breakpoint. the genetic analysis provides a detailed genetic map in the vicinity of the mouse x-inactivation center. comparative mapping data from the human x chromosome indicate that the most probable location of ...19901974879
mapping of mouse carbonic anhydrase-3, car-3: another locus in the homologous region of mouse chromosome 3 and human chromosome least six separate genes determining tissue- and organelle-specific isoforms of carbonic anhydrase are known. we have determined the chromosome location of one of these genes, carbonic anhydrase-3 (car-3), in the mouse and carried out a linkage analysis of car-1, car-2, and car-3. car-3 has been assigned to band 3a2 by in situ hybridization. we identified a psti restriction fragment length polymorphism between mus spretus and mus mus domesticus and, by using an interspecific backcross, showed ...19902111277
a multilocus linkage map of mouse chromosome 8.we present a genetic linkage map of mouse chromosome 8 that spans 53 cm and includes eight cloned loci. this map was derived from analysis of 100 progeny of an interspecific backcross between mus spretus and mus musculus domesticus. genes that were mapped in this analysis include l7, plat, lpl, ucp, es, mt-1, um, and tat. this analysis positions a new extremely proximal marker on chromosome 8, which is discussed as a potential candidate gene for the nervous locus. these linkage data will be usef ...19912037298
interspersed repetitive element polymerase chain reaction product mapping using a mouse interspecific backcross.we have developed a rapid method of generating and simultaneously mapping interrepeat polymerase chain reaction products using dna from interspecific backcross animals derived from mating c57bl/6j and mus spretus mice. this method is based on the high degree of b1, b2, and l1 dispersed repeat position polymorphism found between these two species of mouse. we have mapped 13 new loci to 9 different chromosomes and have found no evidence of clustering among these loci. the advantages of this approa ...19912071145
mapping of col3a1 and col6a3 to proximal murine chromosome 1 identifies conserved linkage of structural protein genes between murine chromosome 1 and human chromosome 2q.we have investigated the degree of synteny between the long arm (q) of human chromosome 2 and the proximal portion of mouse chromosome 1. to define the limits of synteny, we have determined whether mouse homologs of seven human genes mapping to chromosome 2q cosegregated with anchor loci on mouse chromosome 1. the loci investigated were neb/neb, eln/eln, col3a1/col3a1, cryg/len-2, fn1/fn-1, vil/vil, and col6a3/col6a3. ren-1,2 and acrg were included as two proximal mouse chromosome 1 anchor loci. ...19901981051
a mouse model of the aniridia-wilms tumor deletion syndrome.deletion of chromosome 11p13 in humans produces the wagr syndrome, consisting of aniridia (an absence or malformation of the iris), wilms tumor (nephroblastoma), genitourinary malformations, and mental retardation. an interspecies backcross between mus musculus/domesticus and mus spretus was made in order to map the homologous chromosomal region in the mouse genome and to define an animal model of this syndrome. nine evolutionarily conserved dna clones from proximal human 11p were localized on m ...19902173141
localization of the properdin factor complement locus pfc to band a3 on the mouse x chromosome.the locus for properdin (properdin factor complement, pfc), a plasma glycoprotein, has been mapped to band a3 of the mouse x chromosome by in situ hybridization to metaphase spreads containing an x;2 robertsonian translocation. the x-linkage of the locus has also been confirmed by analysis of mus musculus x mus spretus interspecific crosses. the xa3 localization for pfc places it in the chromosomal segment conserved between man and mouse which is known to contain at least six other homologous lo ...19902272504
construction and analysis of linking libraries from the mouse x chromosome.a hybrid cell line containing the mouse x chromosome on a human background has been used to construct linking libraries from the mouse x chromosome, and approximately 250 unique eagi and noti clones have been identified. seventy-three clones have been sublocalized onto the x chromosome using interspecific mus spretus/mus domesticus crosses and a panel of somatic cell hybrids carrying one-half of reciprocal x-autosome translocations. the average spacing of the linking clones mapped to date is abo ...19902167288
genomic mapping of murine zp-2 and zp-3, two oocyte-specific loci encoding zona pellucida proteins.the zona pellucida is a unique, oocyte-specific matrix that coats the surface of all mammalian eggs. composed of three sulfated glycoproteins in the mouse (zp1, zp2, and zp3), the zona pellucida facilitates early events in fertilization and protects the embryo during preimplantation development. using dna isolated from hamster-mouse somatic cell hybrids and from c57bl/6j x mus spretus interspecific backcross progeny, zp-2 was located on chromosome 7, 11.3 +/- 3.2 cm distal to tyr, and zp-3 was l ...19901968044
a complex of serine protease genes expressed preferentially in cytotoxic t-lymphocytes is closely linked to the t-cell receptor alpha- and delta-chain genes on mouse chromosome 14.a complex of genes encoding serine proteases that are preferentially expressed in cytotoxic t-cells was shown to be closely linked to the t-cell receptor alpha- and delta-chain genes on mouse chromosome 14. a striking difference in recombination frequencies among linkage crosses was reported. two genes, np-1 and tcra, which fail to recombine in crosses involving conventional strains of mice, were shown to recombine readily in interspecific crosses involving mus spretus. this difference in recomb ...19902307468
towards construction of a high resolution map of the mouse genome using pcr-analysed microsatellites.fifty sequences from the mouse genome database containing simple sequence repeats or microsatellites have been analysed for size variation using the polymerase chain reaction and gel electrophoresis. 88% of the sequences, most of which contain the dinucleotide repeat, ca/gt, showed size variations between different inbred strains of mice and the wild mouse, mus spretus. 62% of sequences had 3 or more alleles. ga/ct and at/ta-containing sequences were also variable. about half of these size varia ...19902377456
the syntenic relationship of proximal mouse chromosome 7 and the myotonic dystrophy gene region on human chromosome 19q.the syntenic relationship of the myotonic dystrophy (dm) gene region on human chromosome 19q and proximal mouse chromosome 7 was examined using an interspecific backcross between c3h/hej-gld/gld mice and mus spretus. segregation analyses were used to order homologs of nine human loci linked with the dm gene. their order from the centromere was prkcg, [apoe, atpa-2, ckmm, d19s19h, ercc-2], cyp2b, mag, lhb. two other murine loci, d7rp2 and ngfg, were also positioned within this interval. homologs ...19902307474
localization of the rhodopsin gene to the distal half of mouse chromosome 6.we have assigned the mouse rhodopsin gene, rho, to chromosome 6 using dna from a set of mouse-hamster somatic hybrid cell lines and a partial cdna clone for mouse opsin. this assignment rules out the direct involvement of the rhodopsin gene in the known mouse mutations that produce retinal degeneration, including retinal degeneration slow (rds, chromosome 17), retinal degeneration (rd, chromosome 5), purkinje cell degeneration (pcd, chromosome 13), and nervous (nr, chromosome 8). segregation of ...19902341153
a molecular genetic linkage map of mouse chromosome 7.the homology between mouse chromosome 7 and human chromosomes 11, 15, and 19 was examined using interspecific backcross animals derived from mating c3h/hej-gld/gld and mus spretus mice. in an earlier study, we reported on the linkage relationships of 16 loci on mouse chromosome 7 and the homologous relationship between this chromosome and the myotonic dystrophy gene region on human chromosome 19. segregation analyses were used to extend the gene linkage relationships on mouse chromosome 7 by an ...19901981054
chromosomal localization of the mouse genes coding for alpha 2, alpha 3, alpha 4 and beta 2 subunits of neuronal nicotinic acetylcholine receptor.the chromosomal localization of four neuronal nicotinic acetylcholine receptor subunit genes was performed by following the mendelian segregation of their corresponding alleles in backcrosses involving the mouse species mus spretus and the laboratory strains c57bl/6 or balb/c. a similar analysis previously performed with muscle nicotinic acetylcholine receptor subunits revealed that the genes coding for the alpha and beta subunits are respectively located on chromosome 2 and 11, whereas the gamm ...19902338144
a genetic linkage map of mouse chromosome 10: localization of eighteen molecular markers using a single interspecific backcross.interspecific mouse backcross analysis was used to generate a molecular genetic linkage map of mouse chromosome 10. the map locations of the act-2, ahi-1, bcr, braf, cdc-2a, col6a-1, col6a-2, cos-1, esr, fyn, gli, ifg, igf-1, myb, pah, pgcha, ros-1 and s100b loci were determined. these loci extend over 80% of the genetic length of the chromosome, providing molecular access to many regions of chromosome 10 for the first time. the locations of the genes mapped in this study extend the known region ...19901975791
construction of a detailed molecular map of the mouse x chromosome by microcloning and interspecific crosses.a large number of microclones obtained by microdissection of the mouse x chromosome have been mapped using an interspecific mus domesticus/mus spretus cross. clones displaying close linkage to a number of loci of known phenotype but unknown gene product, such as mdx (x-linked muscular dystrophy), have been obtained. over a central 30 cm span of the mouse x chromosome, 17 clones have been mapped and ordered at a sufficient density to contemplate the complete physical mapping of this region that w ...19872448136
sex, strain, and species differences affect recombination across an evolutionarily conserved segment of mouse chromosome 16.a region of substantial genetic homology exists between human chromosome 21 (hsa21) and mouse chromosome 16 (mmu16). analysis of 520 backcross animals has been used to establish gene order in the homologous segment. d21s16h and mx are shown to represent the known proximal and distal limits of homology between the chromosomes, while gap43, whose human homolog is on hsa3, is the next proximal marker on mmu16 that has been mapped in the human genome. recombination frequencies (rfs) in four interval ...19901981993
specific sequence deletions in two classes of murine leukemia virus-related proviruses in the mouse genome.characteristic long terminal repeats (ltr) of approximately 700 and 750 bp were found, respectively, in the two classes (polytropic and modified polytropic) of murine leukemia virus (mulv)-related nonecotropic nonxenotropic proviral sequences in eight individual molecular clones of rfm/un mouse chromosomal dna fragments. three proviral clones, two polytropic and one modified polytropic, contained sequence deletions in the viral structural genes. nucleotide sequence analysis revealed that 7-bp di ...19892536981
y chromosome evolution in the subgenus mus (genus mus).a 305 base pair dna sequence isolated from the y chromosome of the inbred mouse strain c57bl/10 was used to investigate the pattern and tempo of evolution of y chromosome dna sequences for five species in the subgenus mus, including mus spretus, mus hortulanus, mus abbotti, mus musculus and mus domesticus. variation in hybridization patterns between species was characterized by differences in fragment lengths of both intensely and faintly hybridizing fragments, whereas variation in hybridization ...19892543607
a comprehensive genetic map of murine chromosome 11 reveals extensive linkage conservation between mouse and human.interspecific backcross animals from a cross between c57bl/6j and mus spretus mice were used to generate a comprehensive linkage map of mouse chromosome 11. the relative map positions of genes previously assigned to mouse chromosome 11 by somatic cell hybrid or genetic backcross analysis were determined (erbb, rel, 11-3, csfgm, trp53-1, evi-2, erba, erbb-2, csfg, myhs, cola-1, myla, hox-2 and pkca). we also analyzed genes that we suspected would map to chromosome 11 by virtue of their location i ...19892567264
genetic basis of x-y chromosome dissociation and male sterility in interspecific hybrids.a high frequency of x-y chromosome dissociation (95%) was found at first meiotic metaphase (mi) in spermatocytes of interspecific hybrids between laboratory mice, c57bl/6j (bl/6) and mus spretus, compared with an x-y dissociation frequency of only 3-4% in parental mice. the x-y dissociation in f1 hybrids occurred before diakinesis rather than as a precocious dissociation at mi. the high x-y dissociation was accompanied by spermatogenic breakdown after mi, resulting in male sterility. all f1 male ...19912052565
inactivation of the zfx gene on the mouse x chromosome.zfx, an x chromosome-linked gene encoding a zinc-finger protein, has previously been shown to escape x inactivation in humans. here we report studies of the inactivation status of the homolog, zfx, on the mouse x chromosome. we took advantage of both the preferential inactivation of the normal x chromosome in females carrying the t(x;16)16h translocation and the high degree of nucleotide sequence variation between the laboratory strain of mouse [corrected] and mus spretus genomes. an ecorv restr ...19912052543
a molecular genetic linkage map of mouse chromosome 4 including the localization of several proto-oncogenes.we have constructed a 64-cm molecular genetic linkage map of mouse chromosome 4 using interspecific backcross animals derived from mating c57bl/6j and mus spretus mice. several proto-oncogenes and common sites of viral integration have been assigned regional locations on chromosome 4 including mos, lyn, jun, lmyc, lck, fgr, and dsi-1. additional loci mapped in this study to chromosome 4 were tsha, mup-1, rrm2-ps1, ifa, and anf. a comparison of our mapping data with inbred strain mapping data did ...19892574141
localization of two alkaline phosphatase genes to the proximal region of mouse chromosome 1.using a human cdna clone encoding the intestinal form of alkaline phosphatase, we have identified and mapped by rflp analysis in a mus spretus x c57bl/6j interspecies backcross two alkaline phosphatase genes which segregate independently on the proximal part of mouse chromosome 1. the gene order and intergene distances were determined by standard backcross analysis as: centromere- len-2 - 19.0 cm - akp-3 - 20.0 cm - akp-4 - 2.0 cm - ren-1.19892575484
linkage analysis of the murine mos proto-oncogene on chromosome 4.a linkage analysis of the murine mos gene, which codes for the c-mos proto-oncogene, was performed in 88 backcross progeny of an interspecies cross of laboratory mice and mus spretus. linkage was tested for four different genes on mouse chromosome 4: aco-1, mup-1, b, and ifb. the gene order (from centromere) with intervening percentage recombination is mos-15.9 (+/- 3.9)-aco-1-5.6 (+/- 2.4)-mup-1-3.4 (+/- 1.9)-b-5.6 (+/- 2.4)-ifb. these results confirm the previous assignment of mos to chromosom ...19892568329
the murine il-6 gene maps to the proximal region of chromosome 5.murine il-6 cdnas were isolated by cross-hybridization with a human il-6 cdna from an il-1 activated bone marrow stromal cell line (w20). mouse-hamster somatic cell hybrids were utilized to localize murine il-6 to chromosome 5. genetic mapping with respect to en-2, albh, and gus in backcross progeny from an interspecific mating between c57bl/6j and mus spretus positioned il-6 3 cm distal to en-2. the syntenic relationships of il-6 and en-2 in mouse and man, as well as the potential role of il-6 ...19892563387
a repeated segment on the mouse y chromosome is composed of retroviral-related, y-enriched and y-specific sequences.we report the isolation and characterization of two recombinant clones containing dna derived from the y chromosome of the c57bl/10 inbred mouse strain. both clones were isolated from a lambda phage library derived from a partial ecori digest of c57bl/10 male dna using the murine retrovirus m720. characterization of these clones showed they were derived from a repeated segment present on the c57bl/10j y chromosome that contains sequences found elsewhere in the genome. in addition, one clone cont ...19892731728
mapping of abll within a conserved linkage group on distal mouse chromosome 1 syntenic with human chromosome 1 using an interspecific cross.a human abelson related gene (abll) cdna clone was used to detect restriction fragment length polymorphisms (rflps) on mouse southern blots. abll was mapped to mouse chromosome 1 by analysis of segregation with other distal chromosome 1 genetic polymorphisms by using a panel of dnas from [(c3h/hej-gld/gld x mus spretus) f1 x c3h/hej-gld/gld] interspecific backcross mice. the data indicate the following gene order: (centromere)-cd45-6.5 cm-lamb-2-1 cm-abll-2 cm-at-3. the results extend the analys ...19892567701
localization of the muscle, liver, and brain glycogen phosphorylase genes on linkage maps of mouse chromosomes 19, 12, and 2, respectively.mammalian glycogen phosphorylases comprise a family of three isozymes, muscle, liver, and brain, which are expressed selectively and to varying extents in a wide variety of cell types. to better understand the regulation of phosphorylase gene expression, we isolated partial cdnas for all three isozymes from the rat and used these to map the corresponding genes in the mouse. chromosome mapping was accomplished by comparing the segregation of phosphorylase restriction fragment length polymorphisms ...19892575583
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