nucleoside phosphorylase 2 (np-2) of mice.isozyme patterns of nucleoside phosphorylase (np) in 16 inbred strains, two recombinant inbred, one congenic, and three species of wild mice were studied. evidence is provided for a genetic locus, np-2, encoding an electrophoretic variant which is expressed exclusively in erythrocytes of certain inbred strains. this finding establishes the occurrence of genetic polymorphism of np among inbred strains of mice. in addition, the npla allele previously reported only in inbred strains has been observ ...1978109081
genetics of hydroxyacid oxidase isozymes in the mouse: localisation of hao-2 on linkage group xvi.electrophoretic variants of individual isozymes of l-alpha-hydroxyacid oxidase (haox-b4) and amylase (amy-a) in an asian subspecies of mouse, mus musculus castaneus, have been used to localise the gene encoding the haox b subunit. the structural gene loci for these isozymes (hao-2 and amy-1) are apparently linked (4.9 +/- 2.4 per cent recombinants) in this organism, which places hao-2 on linkage group xvi, since previous studies by eicher and co-workers (1976) have localised amy-1 on this chromo ...1978284003
rejection of c57bl/6 skin grafts by (c57bl/6 x mus musculus castaneus)f1 hybrids.hybrid offspring from c57bl/6(b6) females mated to males of the subspecies mus musculus castaneus received b6 skin grafts. no strong y chromosome-linked histocompatibility genes were detected, although occasional rejection of parental grafts by both male and female hybrids was observed after long periods. rejection was attributed to interaction of b6 and castaneus-derived genes in the hybrids.1979375495
the genetics and developmental regulation of l-glycerol 3-phosphate addition to reviewing the genetic regulation of l-glycerol 3-phosphate dehydrogenase during development in the mouse, new evidence is presented that the electrophoretic properties of l-glucerol 3-phosphate dehydrogenase in mus castaneus are determined by an allele (d) at the gdc-1 locus. accordingly there are three alleles at the gdc-1 locus; the b allele in c57bl/6j mice differs from the d allele in electrophoretic properties and the c allele in balb/cj mice differs from the d allele with re ...19751123411
isolation and initial characterization of a large repeat sequence element specific to mouse chromosome 8.a clone containing 15.6 kb of mouse genomic dna was specifically localized to murine chromosome 8 by fluorescence in situ hybridization. the major signal, mapping just below the centromeric heterochromatin, was much too intense for a single-copy probe. two additional weak hybridization signals were detected in or near distal bands 8b3 and 8d. six subclones spanning the entire 15.6-kb insert gave strong centromere proximal signals; however, none of these clones cross-hybridized with each other, s ...19921559702
genetic variation in mouse apolipoprotein a-iv due to insertion and deletion in a region of tandem repeats.we have detected three unique apolipoprotein a-iv (apoa-iv) charge isoforms in strains of commensal mice. the cdna sequences for one representative of each isoform (mus domestesticus strains c57bl/6j and 129/j and mus castaneus) revealed a polymorphism within a series of four imperfect repeats encoding the sequence glu-gln-ala/val-gln. insertions or deletions of 12 nucleotides within this repetitive region have given rise to three genotypes characterized by three (129), four (c57bl/6), or five ( ...19911648102
genetic mapping of the lurcher locus on mouse chromosome 6 using an intersubspecific backcross.the lurcher (lc) mutant mouse strain exhibits postnatal degeneration of cerebellar purkinje cells. we have typed progeny from an intersubspecific, phenotypic backcross at seven loci to develop a genetic linkage map which spans approximately 35 cm surrounding and including the lc locus on mouse chromosome 6. [(mus musculus castaneus x b6cba-aw-j/a-lc)f1 x b6cba-aw-j/a]n2 progeny were scored visually for the lurcher phenotype and molecularly, through restriction fragment length polymorphism analys ...19911672287
rapid physical mapping of cloned dna on banded mouse chromosomes by fluorescence in situ hybridization.physical mapping of dna clones by nonisotopic in situ hybridization has greatly facilitated the human genome mapping effort. here we combine a variety of in situ hybridization techniques that make the physical mapping of dna clones to mouse chromosomes much easier. hybridization of probes containing the mouse long interspersed repetitive element to metaphase chromosomes produces a giemsa-like banding pattern which can be used to identify individual mus musculus, mus spretus, and mus castaneus ch ...19921733847
ectoparasites of commensal rodents in sulawesi utara, indonesia, with notes on species of medical importance.ectoparasite records are presented for four species of commensal murid rodents (rattus rattus palelae miller & hollister, r. argentiventer (robinson & kloss), r. exulans (peale) and mus musculus castaneus waterhouse) in sulawesi utara, with particular reference to the potential for these arthropods to bite and transmit pathogens to humans. the flea, xenopsylla cheopis (rothschild), was most common on r.r. palelae and is capable of transmitting plague and other pathogens to humans although no cur ...19911768888
dominant expression of a distinctive v14+ t-cell antigen receptor alpha chain in mice.a distinctive variable region 14-positive (v14+) alpha chain (v alpha 14+) of the t-cell antigen receptor is predominantly expressed in multiple mouse subspecies. the v alpha 14 family has two members, v alpha 14.1 and v alpha 14.2, which differ by only three amino acids at positions 50-52. based on the ecori restriction fragment length polymorphism of the gene encoding v alpha 14, mice can be divided into three groups: type i with an 11.2-kilobase (kb) fragment, type ii with a 2.0-kb fragment, ...19911881891
recombinational hotspot specific to female meiosis in the mouse major histocompatibility complex.the wm7 haplotype of the major histocompatibility complex (mhc), derived from the japanese wild mouse mus musculus molossinus, enhances recombination specific to female meiosis in the k/a beta interval of the mhc. we have mapped crossover points of fifteen independent recombinants from genetic crosses of the wm7 and laboratory haplotypes. most of them were confined to a short segment of approximately 1 kilobase (kb) of dna between the a beta 3 and a beta 2 genes, indicating the presence of a fem ...19901968049
characterization of the molecular defects in the mouse e beta f and e beta q genes. implications for the origin of mhc polymorphism.the e beta f and e beta q genes have been isolated and sequenced to investigate the molecular basis for their defective expression. a previous study from this laboratory, which characterized the expression of these genes at the rna level, showed both genes to have defects in posttranscriptional rna processing. in this paper, the defect in the e beta q gene from the inbred mouse strain b10.g (mus musculus domesticus) is shown to be a single base insertion in the rna donor splice site of the first ...19902106558
genetic variation for enzyme structure and systemic regulation in two new haplotypes of the beta-glucuronidase gene of mus musculus castaneus.two new haplotypes of the [gus] gene complex have been characterized following their transfer from mus musculus castaneus, where they were found, to a c57bl/6j genetic background. the [gus]cs haplotype carries a new structural allele, gus-scs, coding for enzyme with decreased thermolability and lacking an antigenic site present in other beta-glucuronidase allozymes. the [gus]cl haplotype carries another new structural allele, gus-scl, that codes for enzyme with increased thermolability and posse ...19852413015
genetic differences in the histochemically defined structure of oligosaccharides in mice.a wide range of tissues from three interfertile species of mice and an interspecific hybrid was examined with lectins conjugated to peroxidase to localize specifically glycoconjugates containing terminal alpha-n-acetylgalactosamine, alpha-galactose, and alpha-fucose, and the terminal disaccharide galactose-(beta 1----3)-n-acetylgalactosamine. this battery of lectins disclosed marked heterogeneity of glycoconjugates in different histological sites in a given animal and even between cells in a pre ...19872443557
ly-2.3 antigen derived from subspecies of the asian mouse (mus musculus castaneus). 19882458316
molecular basis for the defective expression of the mouse ew17 beta gene.four of the eleven independent h-2 haplotypes of inbred mouse strains and approximately 15% of wild mouse chromosomes 17 fail to express the e alpha e beta class ii histocompatibility (ia) ag. these e- haplotypes are defective in the expression of the e alpha and/or the e beta chain. none of the e beta defects has previously been described at the molecular level. in this study, we report the molecular basis for the defective expression of the e beta gene from the w17 haplotype of the h-2 congeni ...19882460545
serological survey of complement factor h in common laboratory and wild mice: a new third allotype.antigenic specificities of complement factor h from mice were studied serologically. in addition to previously reported allotypes, referred to as h.1 and h.2, a new allotype of complement factor h, h.3, was identified in the bfm/2ms strain derived from european wild mice. using three different alloantisera raised against the various mouse factor h allotype, a serological survey of the common laboratory strains and wild-derived strains of mus musculus and its relatives, mus spretus, mus spretoide ...19892522412
two genes controlling the expression of extended globoglycolipids in mouse kidney are closely linked to each other on chromosome 19.the gene (gsl-5) controlling the expression of gl-y (gal beta 1-4(fuc alpha 1-3)glcnac beta 1-6(gal beta 1-3)gb4cer) in mouse kidney was suggested to be located near ea-4 on mouse chromosome 19 by the results of glycolipid analysis of bxd/ty recombinant inbred strains (sekine et al. [1987] j. biochem. 101, 563-568). in this study, gsl-5 was mapped on mouse chromosome 19. among 133 backcross progeny produced on mating between dba/2 mice and (wht/ht x dba/2)f1 mice, 10 recombinants between lyt-1 a ...19892787792
the same mhc recombinational hot spots are active in crossing-over between wild/wild and wild/inbred mouse chromosomes.four recombinational breakpoints were mapped in the k-a interval of the mouse major histocompatibility complex (mhc) by southern blot analysis. the breakpoint in b10.sbr, containing a b/s recombinant mhc haplotype, is located about 45 kb upstream of the a beta 2 gene close to the breakpoint in b10.aqr. crossover in two cas3/cas4 and one cas4/cas3 recombinant haplotypes has taken place in the previously identified k/a beta 3 and a beta 3/a beta 2 recombinational hot spots. the same hot spots are ...19882891614
molecular characterization of a meiotic recombinational hotspot enhancing homologous equal crossing-over.we have cloned and sequenced a meiotic recombinational hotspot between the a beta 3 and a beta 2 genes in the major histocompatibility complex (mhc) of the mouse. this recombinational hotspot in the mus musculus castaneus cas3 haplotype was previously localized to a region of 9.5 kb of dna in which five independent crossing-over events occurred at the unusually high frequency of 0.6%. aside from cas3, the hotspot appears to be absent in many other mhc haplotypes. we have now confined the five re ...19863023057
diverse wild mouse origins of xenotropic, mink cell focus-forming, and two types of ecotropic proviral genes.we analyzed wild mouse dnas for the number and type of proviral genes related to the env sequences of various murine leukemia viruses (mulvs). only mus species closely related to laboratory mice carried these retroviral sequences, and the different subclasses of viral env genes tended to be restricted to specific taxonomic groups. only mus musculus molossinus carried proviral genes which cross-reacted with the inbred mouse ecotropic mulv env gene. the ecotropic viral env sequence associated with ...19873041030
intra-h-2 recombination in t haplotypes shows a hot spot and close linkage of 1tw5 to h-2k.the embryonic lethal mutation in the tw5 haplotype is known to map near the h-2k region of the mouse major histocompatibility complex. additional data obtained by classical genetic methods demonstrate that the tw5 lethal gene is effectively inseparable from h-2k. no recombinants were found between h-2k and tw5 in a sample representing over 1200 mice. on a statistical basis tw5 must be less than 250 kb from the h-2k gene. in the course of these mapping studies we obtained a set of 11 intra-h-2 re ...19883378835
altered turnover of hypoxanthine phosphoribosyltransferase in erythroid cells of mice expressing hprt a and hprt b alleles.we have previously shown that mice expressing hprt a allele(s) have erythrocyte hypoxanthine phosphoribosyltransferase (hprt) levels that are approximately 25-fold (mus musculus castaneus) and 70-fold (mus spretus) higher than in mice that express the hprt b allele (mus musculus domesticus; c57bi/6j; c3h/heha), and that these differences in erythrocyte hprt levels are due to differences in the turnover rates of the hprt a and b proteins as reticulocytes mature to erythrocytes. we show here that: ...19873609725
gene organization and recombinational hotspots in the murine major histocompatibility chromosome walking in the major histocompatibility complex (mhc) of the balb/c mouse, we have linked the k region to the i region at the molecular level. forty-nine overlapping cosmid clones define a stretch of about 600 kb of dna containing 2 class i and 7 class ii genes. eleven independent recombination events were mapped between the k and the i region marker loci by southern blot analysis of polymorphic restriction sites. eight of these events involved crossing-over, at an unusually high f ...19863754180
elevated levels of erythrocyte hypoxanthine phosphoribosyltransferase associated with allelic variation of murine hprt.murine stocks with wild-derived hypoxanthine phosphoribosyltransferase (hprt) a alleles (hprt a) have erythrocyte hprt activity levels that are approximately 25-fold (mus musculus castaneus) and 70-fold (mus spretus) higher than those of laboratory strains of mice with the common hprt b allele (mus musculus: c3h/heha or c57b1/6). since the purified hprt a and b enzymes have substantially similar maximal specific activities (64 and 46 units/mg of protein, respectively), we infer that these hprt a ...19854074678
relatedness of mouse satellite deoxyribonucleic acid to deoxyribonucleic acid of various mus species.mouse (mus musculus musculus) satellite dna is able to reassociate with repeated dna sequences of mus caroli and mus cervicolor, but low thermal stability of the products indicates significant differences between satellite and related dnas of these two mus species. there appear to be several satellite-related populations in m. caroli dna, each of which forms hybrids of low thermal stability with repeated sequences of m. cervicolor dna. dnas from the subspecies mus musculus molossinus and mus mus ...19734519644
genomes of murine leukemia viruses isolated from wild mice.the genomes of murine leukemia viruses (mulv) isolated from wild mice have been studied. detailed restriction endonuclease maps of the 8.8-kilobase (kb) unintegrated linear viral dnas were derived for five ecotropic and five amphotropic mulv's from california field mice, for friend mulv, and for one ecotropic and one xenotropic mulv from mus musculus castaneus. in general, the california mulv's were similar in their leftward 6 kb (corresponding to the leftward long terminal repeat [ltr], gag, an ...19816270351
cytoplasmic inheritance of a cell surface antigen in the mouse.mta is a cell surface antigen of the mouse and serves as a target for specific t killer lymphocytes. using a killer cell assay, the antigen has been found in 72 strains of laboratory mice and, with one exception, in all tested samples of mice caught in the wild or bred from such, including mus molossinus, mus castaneus and mus spretus. five strains of rats, non-inbred nmri mice, most substrains of nzb mice and the closely related strain nzo are negative for mta. in reciprocal f1 crosses between ...19836341170
atropine esterase status of laboratory mice.fifty eight inbred strains of laboratory mice and two species of wild mice [mus castaneus (inbred) and peromyscus maniculatus bairdii (closed colony)] were tested for the presence of atropine esterase by the microhematocrit tube test of tucker and beattie. the results show clearly that laboratory mice do not have significant levels of atropine esterase as determined either by the microhematocrit tube test or by the agar plate test, and therefore that under these conditions atropine esterase is n ...19846482377
normal mouse serum contains peptides which induce fibroblasts to grow in soft agar.the untransformed mouse fibroblast cells nih/3t3, c3h/10t1/2, and rat nrk cells do not grow in soft agar in medium supplemented with 10% fetal calf serum. when fetal calf serum in the growth medium was supplemented with less than 1% of sera from mice or other vertebrates, however, these cells responded, forming large colonies. the morphology of soft agar colonies was a function of the treated cell type. in the presence of 10% serum from c57bl/6 mice, nrk cells grew to smooth-surfaced spherical c ...19836603462
genetic variation of milk proteins in mice.a survey for qualitative and quantitative variation in milk proteins from 58 inbred strains of mice revealed two electrophoretic variants. one is in a whey acidic protein of milk of ybr mice and the other is in a curd protein of the asian house mouse, mus musculus castaneus. the whey acidic protein variant is shown to be under the control of a single mendelian autosomal gene with alleles expressed in a codominant manner. this gene is designated wap, is not identical to eg, is not x linked, and i ...19817332524
genetic mapping near the myd locus on mouse chromosome 8.myodystrophy (myd), an autosomal recessive mutation of the mouse characterized by progressive weakness and dystrophic muscle histology, maps to the central portion of chromosome (chr) 8 (lane et al. j. hered 67, 135, 1976). this portion of chr 8 contains the genes for a mitochondrial uncoupling protein (ucp) and kallikrein (kal3), which map to distal 4q in the human, providing evidence for a segment of homology. characteristics of the myd phenotype coupled with this homology suggest that myd may ...19957613034
genetic analyses of tattered, an x-linked dominant, developmental mouse mutation.tattered (td) is an x-linked dominant mouse mutation that causes prenatal lethality in affected males. to map the locus, we analyzed 199 normal male and affected female progeny from a backcross of td and mus castaneus. pedigree analysis of these animals suggests a gene order of cen-dxwas70-(td, dxmit26, gata1, tcfe3)-(cybb, otc)-tel, where tcfe3 is a transcription factor homologous to a gene involved in the murine microphthalmia (mi) mutation [hodgkinson et al. cell 74, 395-404, 1993]. to evalua ...19957613037
neuromuscular degeneration (nmd): a mutation on mouse chromosome 19 that causes motor neuron degeneration.neuromuscular degeneration, nmd, is a spontaneous autosomal recessive mutation in the mouse producing progressive hindlimb impairment caused by spinal muscular atrophy. we used an intersubspecific intercross between b6.bks-nmd2j/+ and mus musculus castaneus (cast/ei) to map the nmd mutation to mouse chromosome (chr) 19 with the most likely gene order: nmd-(d19sel2, pygm)-cntf-pomc2-d19mit16-cyp2c-got1. nmd maps near muscle deficient, mdf, and has a very similar clinical phenotype, but allele tes ...19957749225
origin of lymphocytic-choriomeningitis-virus-bearing mice in japan.mice found in the port areas in japan, where antibody to lymphocytic choriomeningitis virus (lcmv) was detected, may have originated or partially originated from a southern asian subspecies, mus musculus castaneus.19947839750
genetic map of the region around grizzled (gr) and mocha (mh) on mouse chromosome 10, homologous to human 19p13.3.grizzled (gr) is a recessive mouse mutation resulting in a gray coat color and reduced perinatal viability. mocha (mh) is one of several recessive mouse mutants characterized by platelet storage pool disorder, pigment abnormalities, reduced fertility, kidney function deficiencies, and, in some mutants, inner ear and natural killer cell deficiencies. murine platelet storage pool deficient mutants may be models for chediak-higashi and hermansky-pudlak syndromes in humans. the genes for gr and mh a ...19947851892
the tight skin (tsk) mutation in the mouse, a model for human fibrotic diseases, is tightly linked to the beta 2-microglobulin (b2m) gene on chromosome 2.the tsk mutation in the mouse is characterized by the excessive accumulation of collagen in skin and various internal organs, including the heart and lungs. these connective tissue abnormalities are similar to those present in human systemic sclerosis or scleroderma. the tsk mutation provides an opportunity to investigate, at the molecular level, the pathogenesis of tissue fibrosis. as a first step to cloning the tsk gene, we report the localization of the tsk mutation with respect to known mole ...19937902327
microdissection of proximal mouse chromosome 6: identification of rflps tightly linked to the ob a previous report, the ob mutation was mapped to a position 5 cm distal to met on murine chromosome (chr) 6 in tight linkage to cpa. in order to identify additional rflps in the region of ob, we have made use of chromosome microdissection of a 6:16 robertsonian chromosome. in total, 19 rflps were used to type 131 progency of a b6d2 ob/ + x b6 spretus ob/ + intercross. fifteen of the rflps mapped to chr 6, one of which, d6rck13, was tightly linked to ob. for refinement of the genetic map aroun ...19937906968
chromosomal mapping and expression levels of a mouse soluble epoxide hydrolase gene.the chromosomal location of a murine soluble epoxide hydrolase gene was determined using in situ mapping, restriction fragment length polymorphism (rflp) and simple sequence length polymorphism (sslp) analysis. in situ hybridization to mouse metaphase chromosomes using a soluble epoxide hydrolase cdna probe showed that soluble epoxide hydrolase maps at band d of chromosome 14. an rflp found between mus castaneus (cast) and mus musculus (mev) was used to map the soluble epoxide hydrolase gene in ...19947915936
pcr-amplification of simple sequence repeat variants from pooled dna samples for rapidly mapping new mutations of the mouse.a novel strategy for rapidly mapping new mutations of the mouse is presented and tested. the inbred mus musculus castaneus strain cast/ei is used as a tester stock. separate dna pools are made from mutant and wildtype intercross (f2) progeny and analyzed using simple sequence repeat (ssr) variants detected using pcr amplification. linkage is indicated if the mutant dna pool is enriched for the mutant-strain ssr allelic fragment in comparison with the wildtype pool and controls. the expected cont ...19947959741
genetic analysis of liver tumorigenesis in sv40 t antigen transgenic mice implies a role for imprinted genes.liver tumors from interspecific hybrid, transgenic mice containing the sv40 early region linked to a mouse major urinary protein enhancer/promoter were analyzed for loss of heterozygosity to identify chromosomal regions which potentially contain genetic loci involved in multistep tumorigenesis. a broad pattern of complete and partial loss of heterozygosity or allelic imbalance was observed with frequent loss of heterozygosity/partial loss of heterozygosity of loci on chromosomes 1, 5, 7, 8, and ...19947987847
high-resolution mapping of a minor histocompatibility antigen gene on mouse chromosome 2.minor histocompatibility (h) loci are significant tissue transplantation barriers but are poorly understood at the genetic and molecular level. we describe the construction of a high-resolution genetic map that positions a class ii mhc-restricted minor h antigen locus and orders 12 other genes and genetic markers within the we-un interval of mouse chromosome (chr) 2. an intersubspecific backcross between b10.uw/sn-h-3b and cast/ei, an inbred stock of mus musculus castaneus, was used for this pur ...19938118101
genetic control of retroviral disease in aging wild mice.different populations of wild mice (mus musculus domesticus) in los angeles and ventura counties were observed over their lifespan in captivity for expression of infectious murine leukemia virus (mulv) and murine mammary tumor virus (mmtv) and for the occurrence of cancer and other diseases. in most populations of feral mice these indigenous retroviruses were infrequently expressed and cancer seldom occurred until later in life (> 2 years old). mmtv was found in the milk of about 50% of wild mic ...19938125269
the mev mouse linkage testing stock: mapping 30 novel proviral insertions and establishment of an improved stock.continuing efforts to improve the mev linkage testing stock are described. the mev stock carries multiple copies of the ecotropic murine leukemia virus genome. these proviruses are individually detectable in southern blots, making it possible to follow the segregation of many loci simultaneously in genetic crosses. about 50 novel proviral insertions have been detected in this stock and propagated to homozygosity in a number of sublines. many of the proviruses were first observed in individuals t ...19938390965
characterization of a v kappa family in mus musculus castaneus: expansion at the subset level.we have examined the same kappa chain variable (v kappa) region family in several mouse species in order to observe short-term, incremental change at immunoglobulin (ig) multigene loci. in the present study, the igk-v24 family has been characterized in a mus m. castaneus colony derived from individuals originating in thailand and compared to the same family in mus m. domesticus (balb/c) and mus pahari, representing about 1-2 and about 5-9 million years of evolution, respectively. southern hybrid ...19938436416
characterization of a v kappa family in mus musculus castaneus: sequence examine genetic variation at immunoglobulin (ig) multigene loci over short spans of evolutionary time, we have compared members of an ig kappa chain variable (v kappa) region family from several mouse species. in this study, seven unique igk-v24 family members have been isolated from mus m. castaneus and characterized by nucleotide sequence determination for comparison to their counterparts in mus m. domesticus (balb/c), and mus pahari, representing 1-2 million years of evolution in the forme ...19938436417
genetic mapping of the x-linked dominant mutations striated (str) and bare patches (bpa) to a 600-kb region of the mouse x chromosome: implications for mapping human disorders in xq28.striated (str) and bare patches (bpa) are x-irradiation-induced, x-linked dominant mouse mutations that are lethal prenatally in hemizygous males. to map the str mutation, we generated a backcross involving mus castaneus. pedigree analysis of 193 affected female and normal male progeny from the cross places str extremely close to dxmit1 and favors a gene order of (cf-9)-ids-gabra3-dxs1104h-(str, dxmit1)-f8a-dxpas8-dxbay6-dxmit6 for the loci studied. this region of the mouse x chromosome (chr) is ...19938439729
a b2 repeat insertion generates alternate structures of the mouse muscle gamma-phosphorylase kinase gene.a variety of cdna and genomic clones for the gamma-subunit of mouse muscle phosphorylase kinase (phk-gamma m) have been isolated and characterized. the murine gene for phk-gamma m (phkg) exhibits multiple transcription start sites that are identical in skeletal muscle, cardiac muscle, and brain. the gene is composed of 10 exons and includes a 4.9-kb intron located in the 5' untranslated region. two mrna species of 1.75 and 2.55 kb are produced from phkg in icr and c57bl/10 mice; these transcript ...19938486349
genetic basis for resistance to polytropic murine leukemia viruses in the wild mouse species mus castaneus.cultured cells derived from the wild mouse species mus castaneus were found to be uniquely resistant to exogenous infection by polytropic mink cell focus-forming (mcf) murine leukemia viruses (mulvs). this mcf mulv resistance is inherited as a genetically recessive trait in the progeny of f1 crosses between m. castaneus and mcf mulv-susceptible laboratory mice. examination of the progeny of backcrosses demonstrated that susceptibility is inherited as a single gene which maps to chromosome 1. the ...19968551621
a new spontaneous allele at the pink-eyed dilution (p) locus discovered in mus musculus castaneus.mutant mice characterized by a cream coat and pink eyes were spontaneously discovered among the descendants of indonesian wild mice (mus musculus castaneus). this mutant phenotype was controlled by a single autosomal recessive gene that was allelic to the pink-eyed dilution (p) gene. the mutant mouse phenotypically resembled the original p mouse which was the first mutant identified at this locus. nevertheless, these two alleles differed in origin, a previous report suggesting that the original ...19958575552
isolation and characterization of a pseudoautosomal region-specific genetic marker in c57bl/6 mice using genomic representational difference analysis.representational difference analysis was used to identify strain-specific differences in the pseudoautosomal region (par) of mouse x and y chromosomes. one second generation (c57bl/6 x mus spretus) x mus spretus interspecific backcross male carrying the c57bl/6 (b6) par was used for tester dna. dna from five backcross males from the same generation that were m. spretus-type for the par was pooled for the driver. a cloned probe designated b6-38 was recovered that is b6-specific in southern analys ...19958618899
genetics of intracisternal-a-particle-related envelope-encoding proviral elements in mice.intracisternal-a-particle-related envelope-encoding (iape) proviral elements in the mouse genome encode and express an envelope-like protein that may allow transmission of iapes as infectious agents. to test iape mobility and potential transmission in mice, we have analyzed the distribution of iape elements in the genomes of mus spretus and mus musculus inbred strains and wild-caught animals. potential full-length (iape-a) proviral elements are present as repetitive copies in dna from male but n ...19968709280
a novel endogenous mouse mammary tumor virus locus in asian wild mice and its evolutionary divergency.the divergency of endogenous mouse mammary tumor virus (mmtv) in european mouse strains indicates acquisition of exogenous mmtv into their genomes during evolution. in the present study, we selected two strains of asian wild mice, cas-hmi (mus musculus castaneus) and sub-kjr (m. m. subspecies) to investigate a possible divergency of endogenous mmtvs among asian mouse strains. southern blot analysis and polymerase chain reaction (pcr) demonstrated that cas-hmi and sub-kjr have the full structure ...19968766928
fine genetic map of mouse chromosome 10 around the polycystic kidney disease gene, jcpk, and ankyrin 3.a chlorambucil (chl)-induced mutation of the jcpk (juvenile congenital polycystic kidney disease) gene causes a severe early onset polycystic kidney disease. in an intercross involving mus musculus castaneus, jcpk was precisely mapped 0.2 cm distal to d10mit115 and 0.8 cm proximal to d10mit173. in addition, five genes, cdc2a, col6a1, col6a2, bcr, and ank3 were mapped in both this jcpk intercross and a (balb/c x cast/ei)f1 x balb/c backcross. all five genes were eliminated as possible candidates ...19968812475
fine genetic mapping of the hyp mutation on mouse chromosome x.the hypophosphatemic (hyp) mouse is the murine homolog of hypophosphatemic vitamin-d-resistant rickets (hyp) in human. despite extensive investigations in the hyp mouse, the pathophysiology of this x-linked dominant disorder remains unclear. as a first step toward cloning the hyp gene, we have generated a high-resolution linkage map in the vicinity of the hyp locus using two independent backcross panels segregating the hyp mutation, one generated from an interspecific mating between c57bl/6j-hyp ...19968833143
a high-resolution genetic map around waltzer on mouse chromosome 10 and identification of a new allele of waltzer.a new autosomal recessive mouse mutation characterized by deafness and circling behavior was recovered during mutagenesis experiments with chlorambucil (chl). on the basis of allelism tests and linkage analyses, this mutation appears to represent a new allele of waltzer (v) that maps to mouse chromosome (chr) 10. we have designated this new allele, albany waltzer (valb). a high-resolution map of the region around v was constructed from data from two intersubspecific backcrosses involving mus mus ...19979021139
cerebellar deficient folia (cdf): a new mutation on mouse chromosome 6.cerebellar deficient folia, cdf, is a spontaneous autosomal recessive mutation in the mouse with unique pathology; the cerebellar cortex of the cdf/cdf mouse has only 7 folia instead of 10, which is the normal count for the c3h/hej strain in which this mutation arose. the cerebellum of the cdf/cdf mouse is hypoplastic and contains mineral deposits in the ventral vermis that are not present in controls. we used an intersubspecific intercross between c3h/hesnj-cdf/+ and mus musculus castaneus (cas ...19979060409
identification and genetic mapping of a new polycystic kidney disease on mouse chromosome 8.we report here a new mouse mutation, kat, that causes pleiotropic effects including facial dysmorphism, dwarfing, male sterility, anemia, and progressive polycystic kidney disease. kat (kidney anemia and testis) and a second allele, kat2j, that occurred on c57bl/ 6j were mapped to mouse chromosome (chr) 8 using intra- and intersubspecific intercrosses. a high-resolution map for kat2j on chr 8 was constructed using the f2 progeny from a cross between c57bl/6j-kat2j/+ and an inbred strain of mus m ...19979070925
the gene encoding protein 4.2 is distinct from the mouse platelet storage pool deficiency mutation pallid.previous studies identified the gene encoding the erythrocyte membrane protein 4.2 (epb4.2) as a candidate for the mouse mutation pallid (pa); epb4.2 genetically colocalized near pa on mouse chromosome 2, and a truncated epb4.2 transcript was present in tissues derived from pallid mice. we report here evidence that epb4.2 and pa are not allelic. the pallid cdna and intron/exon boundaries show no significant variation from the known balb/c and c57bl/6j epb4.2 sequence, and normal immunoreactive 7 ...19979205130
forebrain overgrowth (fog): a new mutation in the mouse affecting neural tube development.forebrain overgrowth, fog, is a spontaneous autosomal recessive mutation in the mouse producing forebrain, lumbo-sacral, and facial defects. the defects appear to result from excessive growth or cellular proliferation leading to abnormalities in neural tube closure. three unique features of the mutant are: (1) the growth of telencephalon cells into the surrounding mesenchyme, (2) presence of an encephalocele through the midline cleft in some mutants, and (3) dissociation of the tail defect from ...19979216040
mouse mammary tumor virus/v-ha-ras transgene-induced mammary tumors exhibit strain-specific allelic loss on mouse chromosome 4.hybrid mice carrying oncogenic transgenes afford powerful systems for investigating loss of heterozygosity (loh) in tumors. here, we apply this approach to a neoplasm of key importance in human medicine: mammary carcinoma. we performed a whole genome search for loh using the mouse mammary tumor virus/v-ha-ras mammary carcinoma model in female (fvb/n x mus musculus castaneus)f1 mice. mammary tumors developed as expected, as well as a few tumors of a second type (uterine leiomyosarcoma) not previo ...19979238034
[natural hybridization between two subspecies of the house mouse, mus musculus domesticus and mus musculus castaneus, near lake casitas, california].the house mouse mus musculus is a polytypic species, distributed worldwide, with three main subspecies: m. m. musculus in the north-east of eurasia, m. m. castaneus in south-east asia, and m. m. domesticus in europe, the near-east, and africa. this last subspecies may also be found in australia and the americas, where it was brought by european colonization. previous studies, however, have shown the presence of specific antiviral determinants of asian origin in a mouse population at lake casitas ...19989549063
use of a reverse transcriptase-polymerase chain reaction assay to analyze allele-specific expression in individual hippocampal neurons.we report here a single-cell rt-pcr assay for allele-specific gene expression that can be used to probe for somatic variability within the cns. such variability, arising from epigenetic (nonmutational) events or somatic mutation early in development, may give clues as to clonal origin and may also affect the inheritance pattern of some cns disorders. as a model system, we used reciprocal f1 hybrids of the cross mus musculus c57bl/6j x mus musculus castaneus. rna was isolated from individual diss ...19989562962
mapping of murine diabetogenic gene mody on chromosome 7 at d7mit258 and its involvement in pancreatic islet and beta cell development during the perinatal period.mutation of the murine maturity-onset diabetes mellitus of the young (mody) locus induces diabetes, but the effects of its homozygosity on the pancreas remain unknown. f2 mice were obtained by f1 (diabetic c57bl6 x normal mus musculus castaneus) crosses. about 20% of the f2 progeny developed diabetes by 2 wk of age, 50% of the progeny were normal at 2 wk and developed diabetes between 5 and 8 wk of age, and the remaining 30% did not develop diabetes. quantitative trait locus analysis using blood ...19989593767
fine genetic and comparative mapping of the deafness mutation ames waltzer on mouse chromosome 10.the ames waltzer (av) mouse mutant is an autosomal recessive deafness mutation on mouse chromosome 10. previously, av had not been mapped relative to molecular markers. we have performed an intersubspecific backcross with mus musculus castaneus and mapped microsatellite markers in this cross. toothpick pcr on previously frozen tissue samples from offspring was used as an efficient strategy to screen a large number of animals quickly. in 1258 progeny tested we found three recombinants for each of ...19989653653
neuronal and behavioral differences between mus musculus domesticus (c57bl/6jby) and mus musculus castaneus (cast/ei).previous studies have demonstrated that classical inbred strains of laboratory mice do not exhibit large genetic distances when simple sequence repeats (ssrs) are used to test for their polymorphisms whereas mice from wild origin exhibit high polymorphisms (more than 90%) for these sequence when compared with classical inbred strains of laboratory mice. the difference between mus musculus castaneus and c57bl/6j reaches 98% and f1s male and female are fertile. these two properties pave the way fo ...19989754885
strain-dependent developmental relaxation of imprinting of an endogenous mouse gene, kvlqt1.genomic imprinting is an epigenetic modification of the gamete or zygote leading to parental origin-specific differential expression of the two alleles of a gene in somatic cells of the offspring. we previously reported that the human kvlqt1 gene is imprinted and disrupted in patients with germline balanced chromosomal rearrangements and beckwith-wiedemann syndrome. in human, the gene is imprinted in most fetal tissues except the heart, and kvlqt1 is part of a 1-mb cluster of imprinted genes on ...19989799609
receptor-mediated interference mechanism responsible for resistance to polytropic leukemia viruses in mus castaneus.the asian mouse mus castaneus is resistant to infection by the polytropic mink cell focus-inducing (mcf) subgroup of murine leukemia viruses (mulvs). genetic crosses showed this recessive resistance to be governed by a single gene that maps at or near the gene encoding the polytropic viral receptor, rmc1. to investigate this resistance, we mated m. castaneus with mice carrying the wild mouse sxv variant of the rmc1 receptor that allows infection by xenotropic as well as polytropic virus. unlike ...199910196266
status of genomic imprinting in mouse spermatids.the advent of human round spermatid microinjection (rosi) into oocytes as a treatment for severe male infertility raises the question of whether spermatids have undergone all of the maturation processes necessary for normal development. it is particularly important to know whether spermatids have undergone correct genomic imprinting, which results in the parent-of-origin-specific expression of only one allele of a gene. we assessed the imprinting status of three maternally and three paternally e ...199910221240
the role of components of recombination signal sequences in immunoglobulin gene segment usage: a v81x has long been appreciated that some immunoglobulin (and t-cell receptor) gene segments are used much more frequently than others. the vhsegment v81x is a particularly striking case of overusage. its usage varies with the stage of b-cell development and with the strain of mice, but it is always high in b cell progenitors. we have found that the coding sequence and the recombination signal sequences (rss) are identical in five mouse strains, including cast/ei, a strain derived from the species ...199910325418
reduced nucleotide variability at an androgen-binding protein locus (abpa) in house mice: evidence for positive natural selection.previous work has shown that the gene for the alpha subunit of androgen-binding protein, abpa, may be involved in premating isolation between different subspecies of the house mouse, mus musculus. we investigated patterns of dna sequence variation at abpa within and between species of mice to test several predictions of a model of neutral molecular evolution. intraspecific variation among 10 mus musculus domesticus alleles was compared with divergence between m. m. domesticus and m. caroli for a ...199910486974
polymorphisms of the cell surface receptor control mouse susceptibilities to xenotropic and polytropic leukemia viruses.the differential susceptibilities of mouse strains to xenotropic and polytropic murine leukemia viruses (x-mlvs and p-mlvs, respectively) are poorly understood but may involve multiple mechanisms. recent evidence has demonstrated that these viruses use a common cell surface receptor (the x-receptor) for infection of human cells. we describe the properties of x-receptor cdnas with distinct sequences cloned from five laboratory and wild strains of mice and from hamsters and minks. expression of th ...199910516044
partial rescue of the ocular retardation phenotype by genetic modifiers.the or(j) allele of the murine ocular retardation mutation is caused by a premature stop codon in the homeodomain of the chx10 gene. when expressed on an inbred 129/sv strain, the or(j) phenotype is characterized by microphthalmia and a thin, poorly differentiated retina in which the peripheral portion is affected to a greater extent than the central portion. such mutant retinae lack differentiated bipolar cells and the optic nerve typically fails to form, leading to blindness. here, we show tha ...200010640330
germline and somatic loss of function of the mouse cpk gene causes biliary ductal pathology that is genetically modulated.the mouse cpk mutation is the most extensively characterized murine model of polycystic kidney disease (pkd) and closely resembles human autosomal recessive pkd (arpkd), with the exception that b6-cpk/cpk homozygotes do not express the biliary ductal plate malformation (dpm) lesion. however, homozygous mutants from outcrosses to other strains, e.g. dba/2j (d2), cd-1, balb/c and mus mus castaneus (cast), express the dpm. the current study was designed: (i) to characterize the cpk-associated bilia ...200010749984
developing a new model for non-insulin dependent diabetes mellitus (niddm) by using the philippine wild mouse, mus musculus castaneus.the philippine wild-caught castaneus mouse (mus musculus castaneus) and laboratory mouse (c57bl/6j: b6) were used to develop a new non-insulin dependent diabetes mellitus (niddm) model. offspring from the cross between a wild male and b6 female were backcrossed to the sire. one male which exhibited highest fasting hyperglycemia (190 mg/dl) among eighty-seven backcross offspring was selected at 10 weeks of age, and crossed with a b6 female to comprise the fundamental stock (f0). thereafter, full- ...200010803355
a new family of genes and pseudogenes potentially expressing testis- and brain-specific leucine zipper proteins in man and mouse.we have characterized a new mouse gene highly transcribed in the testis, and a derived intronless gene expressed in the embryo. the latter gene is present in mus musculus domesticus and in mus musculus castaneus but is absent in mus spretus. the sequencing of different clones from a testis cdna library reveals a complex transcriptional regulation for the intron-containing gene. the use of several promoters, alternative splicing and trans-splicing, and of two different polyadenylation sites accou ...200010831844
a premature-termination mutation in the mus musculus cyclin-dependent kinase 3 gene.our understanding of the mammalian cell cycle is due in large part to the analysis of cyclin-dependent kinase (cdk) 2 and cdk4/6. these kinases are regulated by e and d type cyclins, respectively, and coordinate the g(1)/s-phase transition. in contrast, little is known about cdk3, a homolog of cdk2 and cell division cycle kinase 2 (cdc2). previous studies using ectopic expression of human cdk3 suggest a role for this kinase in the g(1)/s-phase transition, but analysis of the endogenous kinase ha ...200111172011
virological properties and nucleotide sequences of cas-e-type endogenous ecotropic murine leukemia viruses in south asian wild mice, mus musculus castaneus.two types of endogenous ecotropic murine leukemia viruses (mulvs), termed akv- and cas-e-type mulvs, differ in nucleotide sequence and distribution in wild mouse subspecies. in contrast to akv-type mulv, cas-e-type mulv is not carried by common laboratory mice. wild mice of mus musculus (m. m.) castaneus carry multiple copies of cas-e-type endogenous mulv, including the fv-4(r) gene that is a truncated form of integrated mulv and functions as a host's resistance gene against ecotropic mulv infec ...200111333885
mice with mutations in the mahogany gene atrn have cerebral spongiform changes.a new mutation characterized by mahogany coat color, sprawling gait, tremors, and severe vacuolization of cerebrum, brainstem, granular layer of cerebellum and spinal cord was discovered in a stock of mus castaneus mice. tests for allelism using mice homozygous for 2 known mahogany attractin (atrnmg) mutants showed that the new mutation was an allele of atrnmg. northern analysis showed no expression of atrn in the new mutants. southern analysis strongly suggested that the new mutation deleted mo ...200111444801
frequency and distribution of t-haplotypes in the southeast asian house mouse (mus musculus castaneus) in taiwan.t-haplotypes are a meiotic drive system found on the 17th chromosome of the house mouse (mus musculus). they can be found in wild populations of all four genetically differentiated subspecies. the drive phenomenon is male-specific, such that heterozygous males (+/t) show non-mendelian transmission and transmit the t-chromosome to > 90% of their offspring. so far the most comprehensive studies on the frequencies of t-haplotypes in natural populations have been on just one of the subspecies (m. mu ...200111555276
population differentiation and gene flow revealed by microsatellite dna markers in the house mouse (mus musculus castaneus) in taiwan.we analyzed population subdivision and gene flow of the southeast asian house mouse (mus musculus castaneus) in taiwan by using six microsatellite dna markers. seven populations of the house mouse (187 individuals), including one from fukien province in southeastern china, which is separated from taiwan by the taiwan strait, were analyzed in this study. the overall polymorphic level at the six loci was high (he = 0.76) although individual populations varied in their levels of heterozygosity (he ...200212130825
characterization of a polytropic murine leukemia virus proviral sequence associated with the virus resistance gene rmcf of dba/2 mice.the dba/2 mouse rmcf gene is responsible for in vivo and in vitro resistance to infection by the polytropic mink cell focus-forming (mcf) virus subgroup of murine leukemia viruses (mlvs). previous studies suggested that rmcf resistance is mediated by expression of an interfering mcf mlv envelope (env) gene. to characterize this env gene, we examined resistance in crosses between rmcf(r) dba/2 mice and mus castaneus, a species that lacks endogenous mcf env sequences. in backcross progeny, inherit ...200212134027
identification and functional characterization of novel polymorphisms associated with the genes for arylamine n-acetyltransferases in mice.arylamine n-acetyltransferase (nat) polymorphism in humans has been associated with variation in susceptibility to drug toxicity and cancer. in mice, three nat isoenzymes are encoded by nat1, nat2 and nat3 genes. only nat2 has been shown previously to be polymorphic, a single nucleotide substitution causing the slow acetylator phenotype in the a/j strain. we sequenced the nat genes from inbred (cba and 129/ola), outbred (po and to) and wild-derived inbred (mus spretus and mus musculus castaneus) ...200212142728
analysis of candidate imprinted genes linked to dlk1-gtl2 using a congenic mouse line.the study of genomic imprinting requires the use of dna sequence polymorphisms between interfertile mouse species or strains. most commonly, crosses between mus musculus domesticus and mus musculus castaneus or mus spretus animals are used. difficulties arise in the maintenance of these wild-derived mice in conventional animal facilities, however, and can be overcome by the use of a congenic strain for the region under study. we describe here the generation of a new mouse line, congenic for a re ...200212461649
mapping modifiers affecting muscularity of the myostatin mutant (mstn(cmpt-dl1abc)) compact mouse.the hypermuscular compact phenotype was first noted in a line of mice selected for high body weight and protein content. a new line, based on mice showing the compact phenotype, was formed and selected for maximum expression of the compact phenotype. previously we mapped and identified a 12-bp deletion in the myostatin gene, denoted mstn(cmpt-dl1abc), which can be considered as a major gene responsible for the hypermuscular phenotype. genetic analysis revealed that full expression of the hypermu ...200314504233
a natural allele of nxf1 suppresses retrovirus insertional mutations.endogenous retroviruses have shaped the evolution of mammalian genomes. host genes that control the effects of retrovirus insertions are therefore of great interest. the modifier-of-vibrator-1 locus (mvb1) controls levels of correctly processed mrna from genes mutated by endogenous retrovirus insertions into introns, including the pitpn(vb) tremor mutation and the eya1(bor) model of human branchiootorenal syndrome. positional complementation cloning identifies mvb1 as the nuclear export factor n ...200314517553
genetic variation of microsatellite loci in the major histocompatibility complex (mhc) region in the southeast asian house mouse (mus musculus castaneus).major histocompatibility complex (mhc) genes are the most polymorphic loci known for vertebrates. here we employed five microsatellite loci closely linked to the mhc region in an attempt to study the amount of genetic variation in 19 populations of the southeast asian house mouse (mus musculus castaneus) in taiwan. the overall polymorphism at the five loci was high (he = 0.713), and the level of polymorphism varied from locus to locus. furthermore, in order to investigate if selection is operati ...200314620960
polymorphisms of mouse apolipoprotein a-ii: seven alleles found among 41 inbred strains of mice, apolipoprotein a-ii (apoa-ii) associates to form amyloid fibrils in an age-associated manner. we determined the complete nucleotide sequences of the apoa-ii gene (apoa2) cdna in 41 inbred strains of mice including mus musculus domesticus (laboratory mouse), mus musculus castaneus, mus musculus molossinus, mus musculus musculus and mus spretus. among these strains we identified 7 alleles (apoa2a1, apoa2a2, apoa2b, apoa2c, apoa2d, apoa2e and apoa2f). polymorphisms of nucleotides at 15 pos ...200314986479
genetic influences on ovulation of primary oocytes in lt/sv strain mice.a high proportion of lt/sv strain oocytes arrest in meiotic metaphase i (mi) and are ovulated as diploid primary oocytes rather than haploid secondary oocytes. (mus musculus castaneus x lt/svkau)f1 x lt/svkau backcross females were analysed for the proportion of oocytes that arrested in mi and typed by pcr for a panel of microsatellite dna sequences (simple sequence repeat polymorphisms) that differed between strain lt/svkau and m. m. castaneus. this provided a whole genome scan of 86 genetic ma ...200415509702
distal chr 4 harbors a genetic locus (gct1) fundamental for spontaneous ovarian granulosa cell tumorigenesis in a mouse model.the spontaneous development of juvenile-onset ovarian granulosa cell tumors in mice of the swxj-9 recombinant inbred strain is a model for juvenile-type granulosa cell tumors that appear in very young girls. to expedite gene discovery in this mouse model of childhood cancer, we did a gene mapping study with the swxj-9 recombinant inbred strain and the evolutionarily divergent mus musculus castaneus (cast/ei) strain as a mapping partner. our mapping strategy focused on autosomal determinants of s ...200515735010
lack of telomere shortening with age in mouse resting zone chondrocytes.telomeres are hexameric repeat sequences that flank eukaryotic chromosomes. the telomere hypothesis of cellular aging proposes that replication of normal somatic cells leads to progressive telomere shortening which induces replicative senescence. previous studies suggest that growth plate chondrocytes have a finite proliferative capacity in vivo. we therefore hypothesized that telomere shortening in resting zone chondrocytes leads to replicative senescence.200515795509
the effect of an age-related hearing loss gene (ahl) on noise-induced hearing loss and cochlear damage from low-frequency noise.inbred c57bl/6j mice carry two copies of an age-related hearing loss gene (ahl). it has been shown that these mice begin losing high-frequency hearing at two months. several functional studies have reported that the ahl gene renders mice more susceptible to noise-induced hearing loss (nihl) than strains which do not carry this gene [e.g., hear. res. 93 (1996) 181; hear. res. 155 (2001) 82; j. assoc. res. otolaryngol. 2 (2001) 233]. johnson et al. [hear. res. 114 (1997) 83] developed a congenic b ...200515925194
novel paternally expressed intergenic transcripts at the mouse prader-willi/angelman syndrome locus.gene expression profiling was performed on central nervous system (cns) tissue from neonatal mice carrying the t9h translocation and maternal or paternal duplication of proximal chromosomes 7 and 15. our analysis revealed the presence of two novel paternally expressed intergenic transcripts at the prader-willi/angelman syndrome (pw/as) locus. the transcripts were termed pec2 and pec3, for paternally expressed in the cns. imprinting of these transcripts was confirmed by sequencing of rt-pcr produ ...200515965783
rmcf2, a xenotropic provirus in the asian mouse species mus castaneus, blocks infection by polytropic mouse gammaretroviruses.cells from the asian wild mouse species mus castaneus are resistant to infection by the polytropic host range group of mouse gammaretroviruses. two factors are responsible for this resistance: a defective xpr1 cell surface receptor for polytropic murine leukemia viruses (p-mlvs), and a resistance factor detectable only in interspecies hybrids between m. castaneus and mice with an xpr1 variant that permits infection by xenotropic mlvs (x-mlvs) as well as p-mlvs. this second novel virus resistance ...200516014929
further mapping of quantitative trait loci for postnatal growth in an inter-sub-specific backcross of wild mus musculus castaneus and c57bl/6j mice.we performed a quantitative trait locus (qtl) analysis of eight body weights recorded weekly from 3 weeks to 10 weeks after birth and two weight gains recorded between 3 weeks and 6 weeks, and between 6 weeks and 10 weeks in an inter-sub-specific backcross population of wild mus musculus castaneus mice captured in the philippines and the common inbred strain c57bl/6j ( m. musculus domesticus ), to elucidate the complex genetic architecture of body weight and growth. interval mapping identified 1 ...200516174331
the hnd mouse, a nonobese model of type 2 diabetes mellitus with impaired insulin secretion.this study aimed to develop a novel type 2 diabetes model designated the hnd (horio-niki diabetic) mouse, by transferring diabetogenic genes from wild castaneus mice (mus musculus castaneus) captured in the philippines into laboratory mice (c57bl/6j:b6).200516322404
mapping of genetic modifiers affecting the eye phenotype of ocular retardation (chx10or-j) mice.ocular retardation is a recessive murine mutation whose phenotypic expression is greatly affected by genetic background effects. mice of the inbred 129/svj background that are homozygous for the chx10(or-j) mutation are blind and have a thin, poorly differentiated retina and no optic nerve. a backcross between 129/svj and mus musculus castaneus (casa/rk) produced animals that were homozygous for the chx10(or-j) mutation, yet showed a much milder phenotype. such animals, when brother-sister mated ...200616783634
glutamate oxaloacetate transaminase (got) genetics in the mouse: polymorphism of got-1.we have examined a polymorphism for the soluble glutamate oxaloacetate (got-1) isozyme system which was found in the asian mouse mus castaneus. variants of got-1 segregate as though they are controlled by codominant alleles for a single autosomal locus which we have designated got-1. no close linkage of genes for soluble and mitochondrial forms of the enzyme, got-1 and got-2 respectively, was observed. furthermore, no close linkage of got-1 and the loci c, gpi-1, mod-2, mod-1, ld-1, gpd-1, pgm-1 ...197217248564
a growth qtl (pbwg1) region of mouse chromosome 2 contains closely linked loci affecting growth and body composition.previous qtl studies have identified 24 qtls for body weight and growth from 3 to 10 weeks after birth in an intersubspecific backcross mouse population between c57bl/6j and wild mus musculus castaneus that has 60% of the body size of c57bl/6j. the castaneus allele at the most potent qtl (pbwg1) on proximal chromosome 2 retards growth. in this study we have developed a congenic strain with a 44.1-mb interval containing the castaneus allele at pbwg1 by recurrent backcrossing to c57bl/6j. the cong ...200717514348
nt mutation causing laterality defects associated with deletion of turning (nt), a recessive lethal mutation causing left-right and axial patterning defects, was recovered in outbred cd-1 mice. homozygous mutant embryos die at e11.5, exhibiting randomized heart tube looping and a failure to undergo embryonic turning. previous studies showed notochordal defects associated with abnormal expression of hnf3beta, lefty, and nodal. to identify the genetic alteration underlying nt mutants, we intercrossed this mutation into a mus musculus castaneus strain backgroun ...200717551791
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