| induction of dna synthesis by dichloroisoproterenol without initial rise of the camp level in the parotid gland of mouse. | | 1975 | 245 |
| cell density-dependent stimulation of glutamine synthetase activity in cultured mouse teratoma cells. | | 1975 | 246 |
| an evaluation of factors affecting the in vitro bioassay for erythropoietin. | two main aspects of the in vitro mouse foetal liver cell assay for erythroid stimulating factor (esf) in human sera have been investigated. the haem extraction process has been shown to give specific and quantitative recovery of 59fe labelled haem from haemoglobin thus confirming that the material assayed in human sera is stimulating the synthesis of this protein. the extraction procedure can be simplified considerably by prior mixing of the reagents without significantly influencing the results ... | 1975 | 250 |
| conditions for production, and some characteristics, of mycobacterial growth inhibitory factor produced by spleen cells from mice immunized with viable cells of the attenuated h37ra strain of mycobacterium tuberculosis. | mycobacterial growth inhibitory factor (mycoif), found in supernatant fluids of mouse spleen cell cultures that have been stimulated in vitro with homologous antigen, inhibited the intracellular multiplication of virulent tubercle bacilli within normal mouse peritoneal macrophages in vitro. antigenically stimulated h37ra-immunized mouse spleen cells required 72 h of incubation to produce supernatant fluids that would cause intracellular inhibition. supernatant fluids from 48-h mouse spleen cell ... | 1975 | 330 |
| methods to improve detection of pneumococci in respiratory secretions. | simple methods to enhance the detection of pneumococci in respiratory secretions are needed. sheep blood agar containing 5 mug of gentamicin per ml was more often positive (89%) than either standard sheep blood agar (54%) or mouse inoculation (65%) in recovering pneumococci from 62 adult and pediatric patients. in adults, the direct quellung test on sputum smear was a rapid, sensitive method for predicting subsequent pneumococcal isolation by culture (19 of 20 patients, 95%). the quellung test a ... | 1975 | 421 |
| the relation between myosin adenosinetriphosphatase activity and inactivation of myosin under alkaline conditions of heart muscles in mammals of different size. | atpase activity of myosin in the heart muscle of the mouse, rat, guinea-pig, rabbit and pig was studied at neutral ph and under mild alkaline conditions. at neutral ph the atpase activity of myosin is inversely related to body size of the animal species. the decrease of atpase activity of myosin after alkaline preincubation depends on the degree of atpase activity of intact myosin, i.e. myosin from the heart of the mouse exhibits high atpase activity ae same relationship was found, when comparin ... | 1975 | 651 |
| further evidence for a proton pump in mouse kidney phagolysosomes: effect of nigericin and 2,4-dinitrophenol on the stimulation of intralysosomal proteolysis by atp. | | 1975 | 1019 |
| glycerol inhibition of purified and chromatin-associated mouse liver hepatoma rna polymerase ii activity. | | 1975 | 1029 |
| neurochemical studies in a mouse teratoma with neuroepithelial differentiation. presence of cyclic amp, serotonin and enzymes of the serotonergic, adrenergic and cholinergic systems. | a transplantable mouse testicular teratoma (ott 6050) which displays a spectrum of neuroepithelial differentiation was evaluated biochemically for concentrations of cyclic amp (camp), serotonin (5-ht), and enzymes involved in the metabolism of the biogenic amines and acetylcholine. these values were compared between teratomas with neuroepithelial differentiation as the major or minor component and brains of neonatal and adult mice of related strains. camp, 5-ht, tryptophan hydroxylase (tph), aro ... | 1976 | 1140 |
| rosette formation by mouse lymphocytes. iv. fc and c3 receptors occurring together and separately on t cells and other leucocytes. | a method is described for detecting the simultaneous presence of fc and c3 receptors on mouse spleen cells. a proportion of both t cells and non-t cells bear both receptors. both t-cell and non-t-cell fc receptors were blocked by aggregated mouse igg2 to the same degree. c3, but not fc, receptors were blocked by factors present in the serum of irradiated mice or mice undergoing graft-versus-host reaction. thymocytes activated by injection into irradiated f1 hybrid mice, and thymocytes regenerati ... | 1975 | 1166 |
| effect of 3-methylcholanthrene treatment on phenacetin o-dealkylation in several inbred mouse strains. | an increase in the metabolism of phenacetin to n-acetyl-p-aminophenol is correlated with benzoapyrene hydroxylase induction by 3-methylcholanthrene among several inbred mouse strains. while the magnitude of induction of phenacetin o-dealkylation is considerably less than that of benzoapyrene hydroxylation, the data indicate that in mice, the metabolism of these two substrates is under similar regulatory control. | 1975 | 1225 |
| kinetics of the antibody response to type iii pneumococcal polysaccharide (sss-iii). i. use of 125i-labeled sss-iii to study serum antibody levels, as well as the distribution and excretion of antigen after immunization. | a simple method was described for the preparation of 125i-labeled type iii neumococcal polysaccharide (sss-iii) with a high specific radioactivity which retained the physical and immunologic properties of native sss-iii. sss-iii was used to study the serum and tissue levels of antigen, as well as its excretion, after i.p. injection. when an optimally immunogenic dose (0.5 mug) of antigen was given, greater than 90% of the injected antigen was excreted during the first 3 days after injection; how ... | 1976 | 1445 |
| mouse epidermal aryl hydrocarbon hydroxylase. | mouse skin contains a nadph-dependent, aryl hydrocarbon hydroxylase (ahh), which is inducible by polycyclic aromatic hydrocarbons. in general, unsubstituted polycyclic hydrocarbons caused a greater induction of epidermal ahh than substituted one (1,2,3,4-dibenzanthracene greater than 1,2,5,6-dibenzanthracene greater than benz (a)anthracene equal 3-methylcholanthrene greater than 7,12-dimethlbenz (a)anthracene) which did not correlate with their ability to initiate tumors in mouse skin. two diffe ... | 1976 | 1447 |
| assessment in the guinea-pig ileum and mouse vas deferens of benzomorphans which have strong antinociceptive activity but do not substitute for morphine in the dependent monkey. | 1 four benzomorphans which have potent antinociceptive activity in the hot-plate and writhing tests in the mouse but do not suppress or precipitate withdrawal symptoms in the morphine-dependent monkey, have been examined for their pharmacological actions in the guinea-pig ileum and mouse vas deferens. 2 in the guinea-pig ileum their agonist potencies are 1.5 to 400 times greater than that of normorphine of morphine whereas in the mouse vas deferens their potencies relative to morphine are 0.3 to ... | 1975 | 2359 |
| toxicological aspects of dimethyl-ether. | the authors report the results of a series of investigations on the toxic effects produced in mice and rabbits by inhalation of dimethyl-ether. median lethal concentration (lc50) and median lethal time (lt50) were determined in the mouse. also the effects of dme inhalation on some physiological parameters (blood pressure, heart rate, blood gas and ph data) were evaluated in the rabbit. | 1975 | 2485 |
| lymphoid cell fractionation by aggregated immunoglobulin-agarose columns. | fractionation by columns of aggregated rat immunoglobulin (agg ig)-agarose was investigated as a method of separating different populations of lymphoid cells. with rat spleen cells, agg ig columns retained phagocytes, igm- and igg-antibody-forming-cells, cells mediating antibody- or pha-induced lysis of chicken erythrocytes, and specifically immune splenocytes lytic to chicken erythrocytes without exogenous antibody. agg ig columns did not selectively remove 'b lymphocytes' (surface-ig-bearing l ... | 1976 | 2637 |
| properties of cholera toxin- and naf-stimulated adenylate cyclase from mouse thymocytes. | kinetic parameters of mouse thymocyte adenylate cyclase activity were determined. naf and cholera toxin stimulated adenylate cyclase. stimulation by either agent did not change the ph or mg2+ optima relative to control (unstimulated cyclase). the km value for atp of adenylate cyclase stimulated by naf was significantly reduced from control. by contrast, cholera toxin treatment did not change the km relative to control. adenylate cyclase, when stimulated by naf, had an optimum for mn2+ alone, or ... | 1976 | 3217 |
| metabolic and biochemical aspects of heat acclimation in the deer mouse. peromyscus maniculatus sonoriensis. | | 1976 | 3322 |
| transport mechanisms in isolated plasma membranes. nucleoside processing by membrane vesicles from mouse fibroblast cells grown in defined medium. | plasma membrane vesicles were isolated from a subline of l929 mouse fibroblasts grown on defined medium in the absence of serum. these vesicles were not significantly contaminated by mitochondria or endoplasmic reticulum. the isolation procedure, a modification of that originally developed by mckeel and jarett (mckeel, d.w., and jarett, l. (1970) j. cell biol. 44, 417-432) employs mechanical homogenization in isotonic medium followed by differential centrifugation. the resultant plasma membrane ... | 1976 | 2604 |
| guanylate cyclase: assay and properties of the particulate and supernatant enzymes in mouse parotid. | a new, very sensitive, rapid and reliable assay for guanylate cyclase has been established based on conversion of 32pgtp to 32pguanosine 3':5'-monophosphate and its separation on dowex 50 and aluminium oxide columns. the optimum conditions for the assay of mouse parotid guanylate cyclase have been established and using this procedure the properties of the enzyme have been investigated. the enzyme was found in both the particulate and supernatant fractions. the particulate enzyme was activated 12 ... | 1976 | 2469 |
| adjuvant and immunostimulating activities of water-soluble substances extracted from mycobacterium tuberculosis (var. hominis). | water-soluble substances have been extracted from two strains of mycobacterium tuberculosis var. hominis: the native hydrosoluble part (polysaccharide and peptidoglycan), a substance in which the polysaccharide moiety is less abundant than in the latter, the acetylated peptidoglycan and, finally a tetrasaccharide-heptapeptide. all four types of substances, when they were injected together with freund's incomplete adjuvant, exerted an adjuvant effect on the production of delayed-type hypersensiti ... | 1975 | 4166 |
| reciprocal influence of the graft vs. host reaction and pregnancy. | the graft versus host reaction (gvhr) was induced in mouse females-hybrids f1 (cba x c57bl/6) by intravenous injection of suspension of the lymphoid cells of the spleen and of lymphoid nodes from c57bl/6 mouse females. pregnancy resulted from interbreeding of the test females with syngenic males 1--5 days before, and 1--10, 10--20, 30--40 and more than 40 days after the moment of the lymphoid cells injection, aggravated the gvhr induced transplantation disease. at the same time the gvhr under th ... | 1975 | 4174 |
| kinetics of haemopoietic recovery in endotoxin-treated mice. | kinetics of mouse spleen colony forming units were studied after intra-peritoneal injection of 1 mug/blody weight bacterial endotoxin s. typhosa. when these mice were used as unirradiated and sublethally irradiated donors, it was possible to study the effect of the endotoxin injection upon the cells. use of the treated mice as irradiated recipients of normal cells gave information about the host effect. in treated unirradiated mice, the total nucleated cell and the cfu counts were disturbed, and ... | 1976 | 4225 |
| compliance and stability of excised mouse lungs. | | 1976 | 4270 |
| tolerance in early embryo aggregation-derived mouse chimaeras. | | 1976 | 2384 |
| glial fibrillary acidic protein from bovine and rat brain. degradation in tissues and homogenates. | compared with human material glial fibrillary acidic protein isolated from bovine, rat and mouse brain was remarkably homogeneous and migrated as a single band at 54 000 mol. wt. on sodium dodecyl sulfate gel electrophoresis. the protein was extremely susceptible to proteolysis and lower molecular weight components were invariably isolated together with the major species when the brain was not rapidly frozen. further degradation of the 54 000 mol wt. polypeptide in bovine tissues incubated at 24 ... | 1976 | 2294 |
| a method for the quantitation of the localized graft-versus-host response in the mouse by uptake of 125iudr. | | 1975 | 1882 |
| maturation of neuroblastoma cells in the presence of dimethylsulfoxide. | addition of dimethylsulfoxide at concentrations of 1% and 2% (vol/vol) to cells of mouse neuroblastoma clone nie-115 in the confluent phase of growth resulted in the production of morphologically differentiated cultures with extensive process formation. cell maintained in 2% dimethylsulfoxide remained in a stable nondividing condition for periods of up to 4 weeks. a high degree of electrical excitability was found in these cells, but there was no clear correlation of this property with the level ... | 1976 | 1756 |
| neuronal properties of hybrid neuroblastoma x sympathetic ganglion cells. | clonal mouse neuroblastoma cells without tyrosine 3-monooxygenase ec 1.14.16.2; tyrosine hydroxylase; l-tyrosine, tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating) activity were fused with normal cells from embryonic mouse sympathetic ganglia. one of the 37 hybrid cell lines obtained possesses high tyrosine 3-monooxygenase activity and synthesizes dopamine. these cells also have excitable membranes and generate action potentials in response to electrical stimuli. thus hybrid cells, gen ... | 1975 | 1745 |
| the effect of alpha, alpha1-dipyridyl on noradrenaline, dopamine and 5-hydroxytryptamine levels and on dopamine-beta-hydroxylase activity in brain. | the effects of alpha, alpha1-dipyridyl (dp) on noradrenaline (na), dopamine (da) and 5-hydroxytryptamine (5-ht) levels in rat and mouse brain and on dopamine-beta-hydroxylase (dbetah) activity in rat brain have been studied, dp decreases na level and inhibits dbetah activity in a dose dependent manner, without affecting da or 5-ht levels. | 1975 | 1715 |
| a study of tobacco carcinogenesis. xiii. tumor-promoting subfractions of the weakly acidic fraction. | the weakly acidic fraction (waf) of cigarette smoke particulate matter was fractioned by silica get chromatography. we assayed the various primary subfractions for potential tumor-promoting activity by measuring the incorporation of tritiated thymidine into mouse epidermal dna as induced by these subfractions. based on these results and on chemical composition, the primary subfractions, were then combined into four major subfractions and tested on initiated mouse skin for tumor-promoting activit ... | 1975 | 1547 |
| effect of laser irradiation and immunosuppressive treatment on survival of mouse skin allotransplants. | the effect of laser beam on the survival of skin allotransplants has been studied. irradiation of the donor skin and of the recipient graft bed improved the survival of the graft. the graft protecting effect of anti-thymocyte serum was considerably enhanced by laser irradiation. | 1975 | 4941 |
| tolerance in tetraparental mouse chimaeras. | | 1976 | 4965 |
| on the pharmacology of 9,10-dihydro-10-(1-methyl-4-piperidylidene)-9-anthrol (wa 335), a histamine and serotonin antagonist (author's transl). | the substance 9,10-dihydro-10-(1-methyl-4-piperidylidene)-9-anthrol (wa 335) was examined for its antagonistic effects against histamine and serotonin, for its atropine-like properties as well as for a series of other qualities in comparison with cyproheptadine and pimethixene. the anti-histamine and anti-serotonin activities of compound wa 335 on the smooth muscle and the capillary do not only exceed that of cyproheptadine but also that of pimethixene. wa 335 shows an extremely strong binding t ... | 1975 | 1049 |
| molecular nature of beta-galactosidase from different tissues in two strains of the house mouse. | one inbred mouse strain, c57bl/kl, has high galactosidase activities in all tissues while another strain, dba/2/kl, has low activities determined by the bgs locus. beta-galactosidase from these two strains was partly purified by a five-step procedure: acidification, ammonium sulfate precipitation, gel filtration at two phs, and isoelectric focusing. no qualitative differences were found between the enzyme preparations from the two strains. they had identical heat inactivation curves, ph optima, ... | 1975 | 988 |
| amodiaquin accumulation by mouse erythrocytes infected with plasmodium berghei. | 14camodiaquin accumulation by washed erythrocyte preparations was characterized to permit comparisons with chloroquine accumulation. erythrocytes infected with plasmodium berghei cs (chloroquine-susceptible) accumulate amodiaquin by a saturable process that has an apparent dissociation constant for amodiaquin of 7.6 x 10(-8) m and is competitively inhibited by chloroquine, quinine and quinacrine, as is the process of chloroquine accumulation. within experimental error, the k1 of 8 x 10(-7) m est ... | 1975 | 488 |
| in vitro adsorption of diphenoxylate hydrochloride on activated charcoal and its relationship to pharmacological effects of drug in vivo. i. | the adsorption of diphenoxylate hydrochloride, a potent antidiarrheal agent, on activated charcoal powder was studied in vitro. langmuir adsorption isotherms were established at ph 4 and 7, and the maximum adsorption capacity of charcoal for this drug was estimated using these values. activated charcoal modified the bioavailability of diphenoxylate hydrochloride in vivo. the antipropulsive action of diphenoxylate in the mouse was strongly inhibited in the presence of activated charcoal. a compar ... | 1975 | 485 |
| inhibitory effects of antihistamines and antiserotonins on the bone marrow reactions produced by escherichia coli endotoxin in mice. | the bone marrow reactions (that is, decrease of nucleated cell counts and increase of red blood cell counts) of mouse bone were observed 1 hr after injection of endotoxin and peaked after 18 hr. these reactions were significantly inhibited when diphenhydramine, promethazine (antihistamines), chlorpromazine (antiserotonin), or cyproheptadine (antihistamine and antiserotonin) was given 30 min before endotoxin. such bone marrow reactions were also induced with histamine or serotonin and peaked 1 hr ... | 1975 | 442 |
| peroxisome development in the metanephric kidney of mouse. | the relationship of enzymatic activity to organelle development and organelle number during differentiation of the metanephric kidney in the mouse was approached from several experimental directions. biochemical analyses of marker enzymes for peroxisomes (catalase and d-amino acid oxidase), mitochondria (cytochrome oxidase) and lysosomes (acid phosphatase) were performed on kidneys at ages from 17 days prenatal to adult. these data were correlated with a morphometric analysis of populations of p ... | 1975 | 440 |
| defective lysosomal enzyme secretion in kidneys of chediak-higashi (beige) mice. | the beige mouse is an animal model for the human chediak-higashi syndrome, a disease characterized by giant lysosomes in most cell types. in mice, treatment with androgenic hormones causes a 20-50-fold elevation in at least one kidney lysosomal enzyme, beta-glucuronidase. beige mice treated with androgen had significantly higher kidney beta-glucuronidase, beta-galactosidase, and n-acetyl-beta-d-glucosaminidase (hexosaminidase) levels than normal mice. other androgen-inducible enzymes and enzyme ... | 1975 | 408 |
| resolution in affinity chromatography. the effect of the heterogeneity of immobilized soybean trypsin inhibitor on the separation of pancreatic proteases. | by affinity chromatography, trypsins and chymotrypsins from mouse pancreas homogenates have been separated using soybean trypsin inhibitor immobilized on sepharose. the effects of the functional heterogeneity of the adsorbent have been investigated in terms of the resolution obtained. heterogeneity of the adsorbent have been investigated in terms of the resolution obtained. heterogeneity has been found to originate from the following sources: heterogeneity of the ligand before immobilization; al ... | 1976 | 5462 |
| neonatal infection with mouse thymic virus: effects on cells regulating the antibody response to type iii pneumococcal polysaccharide. | mice infected neonatally with mouse thymic virus (ta) were evaluated at different ages with respect to their ability to give a plaque-forming cell (pfc) response to type iii pneumococcal polysaccharide (sss-iii), as well as the degree of amplifier and suppressor thymus-derived (t) cell activity present. b cell activity matured rapidly from 2 to 4 weeks of age and was not affected by ta infection. amplifier t cell activity matured progressively over the first 8 weeks of life and was transiently s ... | 1976 | 5561 |
| in vitro biosynthesis of sialosylgalactosylceramide (g7) by mouse brain microsomes. | a sialytransferase activity which catalyzes the synthesis of sialosylgalactosylceramide (g7) from added galactocerebroside and cmp-n-acetylneuraminic acid has been demonstrated in mouse brain microsomes. the enzyme reaction shows a ph optimum of 6.3 and requires detergents. both mn2+ and ca2+ inhibited the reaction, whereas mg2+ had no effect. the apparent km for galactocerebroside leading to g7 was estimated to be 8.7 x 10(-4) m. the same microsomal preparation also synthesized hematoside when ... | 1976 | 398 |
| malathion a and b esterases of mouse liver-i. | | 1975 | 14 |
| a new differentiation antigen defining a subpopulation of mouse t cells. | | 1976 | 5683 |
| regulation of guanylate and adenylate cyclase activities by lysolecithin. | the guanylate cyclase activity gtp pyrophosphate-lyase (cyclizing), ec 4.6.1.2 in membrane preparations from 3t3 mouse fibroblasts is stimulated approximately 5-fold by lysolecithin at concentrations of 100 mug/ml and above. | 1976 | 5726 |
| some biological activities of rabbit anti-interferon serum. | after prolonged immunization of rabbits with a semipurified mouse interferon preparation in freund's incomplete or al-span-oil adjuvant, a specific interferon-neutralizing immunoglobulin was obtained from antiserum with a capacity of neutralizing about 49000 mouse interferon units per ml. the specific activity of the antiserum and immunoglobulin was confirmed in tests in which the interaction of antibodies with the cell surface was ruled out. the antiserum (and the immunoglobulin) neutralized bo ... | 1976 | 5864 |
| preparation of fv fragment from the mouse myeloma xrpc-25 immunoglobulin possessing anti-dinitrophenyl activity. | the myeloma iga protein produced by plasmacytoma xrpc-25, was isolated by affinity chromatography on dinitrophenyllysine-sepharose. the affinity constant of the intact protein or its fab' toward 2,4-dinitrophenyl-l-lysine (dnp) was found to be 2.6 x 10(5) m-1. in order to prepare an fv fragment (hochman, j., inbar, d., and givol, d. (1973), biochemistry 12, 1130) from this protein, the heavy and light chains were separated and the light chain was digested with trypsin at ph 8.2 to yield half a l ... | 1976 | 4096 |
| enhancement of interferon induction in mice by polycationic modified polypeptides. | combined intraperitoneal treatment of mice with poly i: c and a polycationic modified polypeptide (poly-dmae-glutamine) was investigated. it was established that the presence of appropriate amounts of poly-dmae-glutamine produced markedly enhanced serum interferon levels as compared to those produced by poly i: c alone. similar combinations injected into mice produced full protection against lethal doses of mouse-virulent semliki forest virus. | 1976 | 5874 |
| distribution of double-stranded f2 phage rna in mouse tissues after intraperitoneal administration. | | 1976 | 5877 |
| purification and characterization of mouse interferon with novel affinity sorbents. | several novel selective sorbents for mouse interferon are described that exploit the hydrophobic property and glycoprotein nature of this molecule. low-molecular-weight ligands (hydrocarbons) and high-molecular-weight ligands (bovine serum albumin) immobilized on agarose bind selectively mouse l-cell interferon. the high selectivity of binding is due primarily to a hydrophobic effect, although electrostatic forces are also apparently involved. mouse l-cell interferon binds to immobilized serum a ... | 1976 | 3666 |
| studies on some lipogenic enzymes of cultured myeloid leukemic cells. | the microsomal fraction of m1 cells (an established cell line of myeloid leukemia) was capable of catalyzing acylation of sn-glycerol 3-phosphate by long-chain fatty acyl-coa thioesters. the principal lipid product formed was identified as phosphatidic acid. palmityl-coa, stearyl-coa, and oleyl-coa were more effective acyl donors than linoleyl-coa and arachidonyl-coa. m1 cells and macrophages differentiated from them exhibited similar levels of sn-glycerol 3-phosphate-acylating activity, which w ... | 1976 | 3240 |
| a comparative study on the irreversible binding of labeled halothane trichlorofluoromethane, chloroform, and carbon tetrachloride to hepatic protein and lipids in vitro and in vivo. | 1) after intraperitoneal injection of labeled ccl4, chcl3, and halothane in mice, 14c is preferentially bound to liver endoplasmic protein and lipid. a considerable activity is also associated with mitochondrial constituents. maximal protein binding (nmol/mg): ccl4: 2.8 (0.5 hrs); chcl3: 11.5 (6 hrs); halothane: 5 (6 hrs). lipid binding: ccl4: 6.4 (5 min); chcl3: 8 (4 hrs); halothane: 13.5 (2 hrs). the form of the binding curves in microsomal and mitochondrial protein and lipid differed with the ... | 1975 | 3152 |
| stress-induced response of mouse liver tyrosine aminotransferase after irradiation. | on x-ray irradiated mice with a total dose of 800 r, the reactivity upon an aggressive agent (chloroform stress) administered at various delays after irradiation was studied. the reactivity of the animals has been emphasized by the hepatic induction of the tyrosine aminotransferase and by the adrenal ascorbic acid depletion. from the 3rd day after irradiation both these parameters showed a marked and irreversible decrease. | 1975 | 2939 |
| characteristics of a xenogeneic lymphocyte transfer reaction: its use in the study of graft versus host capability of mouse lymphoid cells from various anatomic sites. | | 1976 | 6151 |
| graft-versus-host response, measured by splenomegaly assay, by populations of allosensitized mouse lymphocytes. | | 1976 | 6152 |
| correlation of mouse tissue distribution of arabinosylcytosine in vivo with enzymatic activities in vitro. | the distribution of arabinosylcytosine (ara-c) and its metabolites has been measured in the liver, small intestine, spleen, and kidney of mice inoculated ip 5-6 days earlier with l1210 leukemia cells. two major metabolites were found in the tissues--the nucleotides and the deaminated inactive product, arabinosyluracil (ara-u). the decay curve of ara-c in most of these tissues was curvilinear; the ara-c half-lives estimated from the terminal phases were 8. 11, 12, and 12 hr for spleen, kidney, in ... | 1976 | 6236 |
| a case of polydactyly with multiple thresholds in the mouse. | | 1975 | 2925 |
| ultrastructural studies on the phagocytosis of the cultured mouse macrophages and the effects of cytochalasin b, colchicine and ph conditions on their morphology. | | 1975 | 2644 |
| activation of murine lymphocytes by cyclic guanosine 3',5'-monophosphate: specificity and role in mitogen activity. | cyclic guanosine 3',5'-monophosphate (cyclic gmp) stimulates nucleic acid synthesis in lymphocytes, and has been implicated as the intracellular effector of the actions of mitogenic agents on these cells. in the present study, we examined the specificity of the mitogenic activity of cyclic gmp and of its 8-bromo (br) derivatives, and the effects of the t cell mitogens, concanavalin a, phytohemagglutinin, and staphylococcal entertoxin b (seb) on the cyclic gmp content and guanylate cyclase activi ... | 1976 | 4115 |
| use of the mouse jumping test for estimating antagonistic potencies of morphine antagonists. | the potencies of 19 reference morphine antagonists have been compared in a modified version of the mouse jumping test. mice were each implanted subcutaneously with one 75 mg pellet of morphine. antagonist challenge took place 72 h later and the incidence of repetitive vertical-jumping was monitored over 1 h. a high pearson correlation coefficient (r = 0.997) was found between quantitative assays based on the total number of jumps per mouse and quantal assays based on mice jumping at least 6 time ... | 1976 | 6688 |
| determination of d-3-hydroxybutyrate dehydrogenase in mouse pancreatic islets with a photokinetic technique using bacterial luciferase. | a sensitive assay for d-3hydroxybutrate dehydrogenase (ec 1.1.1.30) was developed for use with the minute amounts of material obtained from islets of langerhans microdissected from freeze-dried pancreatic sections. nadh formed in the enzyme reaction was determined by photokinetic analysis of the luminescence obtained with bacterial luciferase from achromobacter fishcherii. in this way, accurate determination was obtained with less than 0.1 mug dry weight of islet material. in obese hyperglycemic ... | 1976 | 4298 |
| a comparison between a melanocyte-stimulating hormone inhibitory factor (mif-i) and substances known to activate central dopamine receptors. | the tripeptide, prolyl-leucyl glycine amide, a melanocyte-stimulating hormone inhibitory factor (mif-i), which has been reported to be effective in improving symptoms of parkinson's disease, has been compared with drugs known to activate dopamine receptors in rat and mouse brain. unlike apomorphine, amphetamine and amantadine it was incapable of producing sterotyped behaviour in the rat and unlike 1-dopa it was also ineffective in rats pretreated with the monoamineoxidase inhibitor mebanazine. n ... | 1976 | 4332 |
| further studies on the fertility promoting factor from human seminal plasma. | a small molecular weight substance from human seminal plasma has been further purified by chromatography. the fertility promoting action of this factor on epididymal sperm has been confirmed in mouse in vitro and in vivo. experimental evidence indicates that the factor acts on the sperm and not on the eggs. its possible mode of action is by improving the motility and survival of the epididymal sperm. | 1975 | 4391 |
| a new method using p-benzoquinone for coupling antigens and antibodies to marker substances. | a method using p-benzoquinone for coupling antigens and antibodies to enzymes and erythrocytes is described. the method involves the treatment of proteins (or polysaccharides) at ph 6 or 7 with an excess of p-benzoquinone. after removal of the unreacted reagent by gel filtration, the "activated" proteins were coupled at ph 8-9 with enzymes or erythrocytes. biological activities of the proteins were not substantially modified by this treatment since 80-100% of the antigen binding capacity was fou ... | 1976 | 7179 |
| the presence of paternal h-2 antigens on hybrid mouse blastocysts during experimental delay of implantation and the disappearance of these antigens after onset of implantation. | the presence of paternal h-2 antigens on hybrid mouse blastocysts before and during implantation was investigated by means of the isotope anti-globulin technique. it was found that experimentally delayed blastocysts possess paternal h-2 antigens whereas these antigens can no longer be detected 14 hours after estradiol activation of delayed blastocysts. | 1975 | 4392 |
| the effect of prostaglandins and prostaglandin inhibitors on spermatogenesis. | the effect of the prostaglandin inhibitors, aspirin and indomethacin and of prostaglandins pge1 and pge2 on spermatogenesis in the mature male mouse has been studied. aspirin at 100 mg/kg and at 200 mg/kg, and indomethacin at 1.0 mg/kg given orally twice a day for fifteen days produced a marked increase in spermatogenesis. the number of step 7 spermatids increased significantly over controls at about the same rate in all three groups. no significant changes in seminal vesicle weight or testicula ... | 1975 | 4399 |
| hematopoietic thymocyte precursors. i. assay and kinetics of the appearance of progeny. | a quantitative assay for the hematopoietic precursor of thymocytes has been developed. using this assay the kinetics of appearance of the progeny of transfused bone marrow and spleen cells in the thymus of irradiated (760 r) mice has been studied. precursor cells are seven to eightfold more common in bone marrow than in spleen and are absent from peripheral lymph nodes. they decline in number as the animals age. when hematopoietic cells are injected immediately after lethal irradiation only a sm ... | 1976 | 4575 |
| local renal graft-versus-host reaction: sequential ultrastructural study. | a sequential study has been made of the ultrastructure of local renal graft-versus-host reactions produced in cyclophosphamide-pretreated rats by injection of either allogeneic or xenogeneic (mouse) spleen cells beneath the renal capsule. early in the reaction, immunoblasts were seen between the outer cortical tubules, and there was associated interstitial oedema and tubular degeneration. in places, several small lymphocytes were observed clustered around and in close cytoplasmic contact with in ... | 1975 | 4760 |
| determinants of lung bacterial clearance in normal mice. | the determinants of the lung clearance of streptococcus pneumoniae, klebsiella pneumoniae, escherichia coli, and staphylococcus aureus were studied in normal mice after exposure to an aerosol of viable bacteria and 99mtc-labeled dead bacteria. the fraction of bacteria in lungs that remained viable 4 h after exposure were: s. pneumoniae, 7.3%; k. pneumoniae, 121%; e. coli, 88.5%; s. aureus, 27.6%. the rate of physical removal of bacterial particles (kmc) was determined from the change in lung 99m ... | 1976 | 7575 |
| duodenal immunoglobulin deficiency in graft versus host disease (gvhd) mice. | the small intestine is a well documented target organ in mouse and human gvhd, and diarrhea is a prominent part of the clinical gvhd syndrome. although a plethora of systemic immune deficits has been documented in gvhd, the integrity of the small intestinal immune system has not been investigated. a correlation has not been demonstrated between systemic immune dysfuction and the incidence of lymphomas in mouse gvhd survivors. if gastrointestinal immune deficiency exists in mouse gvhd, its possib ... | 1976 | 7629 |
| partial regain of activity in heterologous recombinants of phosphorylcholine-binding m-components from different species. | recombination experiments were performed with heavy and light chains derived from a waldenström's igm with specificity against phosphorylcholine. the recombinant molecules had an association constant for phosphorylcholine in the same order of magnitude as the native igm; the number of binding sites at saturation was only slightly decreased in the reconstituted molecules, indicating regain of binding activity after recombination of igm heavy and light chains. heterologous recombinants obtained wi ... | 1976 | 7630 |
| neonatal infection with mouse thymic virus. differential effects on t cells mediating the graft-versus-host reaction. | neonatal infection with mouse thymic virus (ta), a murine herpes virus, produced extensive but temporary necrosis of the thymus which was maximal at 10 to 14 days of age. studies of precursor and amplifier cells mediating graft-vs-host (gvh) reactivity of thymocytes, spleen cells (sc), and lymph node cells (lnc) of normal and ta-infected mice were made at 4 and 8 weeks of age. infection with ta resulting in a profound reduction (70 to 80%) in the direct gvh reactivity of thymocytes at both ages; ... | 1976 | 7631 |
| passive serum sickness in the mouse: the role of vasoactive amines in glomerular deposition of immune complexes. | this paper reports a study of the importance of vasoactive amines in glomerular localization of passively administered immune complexes in the mouse. two strains of mice were investigated, one sensitive and the other relatively resistant to the anaphylactogenic effect of intravenously administered immune complexes. the effect on glomerular deposition immune complexes in animals of both strains treated with either vasoactive amine depletors or antagonists leads to the conclusion that in this expe ... | 1976 | 5102 |
| gamma-glutamyl transpeptidase, a lymphoid cell-surface marker: relationship to blastogenesis, differentiation, and neoplasia. | gamma-glutamyl transpeptidase, an enzyme that catalyzes gamma-glutamyl transfer from gamma-glutamyl compounds to amino acid and peptide acceptors, and which is known to be localized in the membranes of many epithelial cells, was found in a variety of lymphoid cells. the lymphoid cell enzyme is located on the cell surface, and exhibits substantially the same substrate specificity as the enzyme found in epithelial cells. human and rat (but not mouse) lymphocyte gamma-glutamyl transpeptidase was st ... | 1976 | 7785 |
| partial denaturation of mouse dna in preparative cscl density gradients at alkaline ph. | a new technique--partial denaturation of dna in equilibrium cscl density gradients at ph 11.4--is used to determine the distribution of intermediate states in the melting of mouse dna. when the technique is applied in the preparative ultracentrifuge, the dna is fractionated according to stability. neutralization of the partially denatured fractions results in the recovery of most of the dna in its native form. the individual fractions are more homogeneous than the total dna: they have decreased ... | 1976 | 5117 |
| the effects of putative neurotransmitters on the resting membrane potential of dissociated brain neurones in culture. | cultures established from mechanically dissociated neonatal mouse brains were found to be suitable for electrophysiological investigation of drug action. during culture most cells were aggregated into either monolayer regions or thick cords joining monolayer regions. a few cells remained isolated. the neurones in the monolayer regions were distinguished from glial cells by differential staining, and were found to be the best subject for intracellular recording. frequency of resting membrane pote ... | 1976 | 5176 |
| the metabolic activation of the carcinogen 1'-hydroxysafrole in vivo and in vitro and the electrophilic reactivities of possible ultimate carcinogens. | administration of 2',3'-3h-1'-hydroxysafrole to rats or mice resulted in the formation of hepatic dna-, ribosomal rna-, and protein-bound 3h derivatives. alkaline digestion of the 3h-protein released 0.1 to 0.3% of the 3h as a derivative that was identified as 3'-methylmercaptoisosafrole by its cochromatography in five solvent systems with the synthetic compound. 1'-hydroxysafrole was metabolized at a low rate by rat and mouse liver cytosols in a 3'-phosphoadenosine 5'-phosphosulfate-dependent r ... | 1976 | 5188 |
| variable response of normal and transformed mouse cells to interferon inducers. | balb/3t3 mouse cells, normal and sv40-transformed, produced interferon when induced with newcastle disease and influenza viruses; transformed cells failed to respond to double-stranded rna inducers. | 1976 | 7948 |
| melatonin induced changes in gonads; pelage and thermogenic characters in the white-footed mouse, peromyscus leucopus. | | 1976 | 5240 |
| a study of h-2 mutations in mice. v. detection of mutations m505, hzl and m506 in the host vs transplant reaction. | h-2 mutant/normal pairs of congenic mouse strains c57bl/6equilibriumb6.m505 (h-2kbd), c57bl/6equilibriumb6.c(hz1) (h-2kba) and a.caequilibriuma.ca(m506) (h-2fa) were studied in the graft versus host assay. all the three mutations were expressed in these tests as gain and loss of h-2 antigen. the data obtained confirm an earlier suggestion that the h-2k antigenic molecule bears antigenic determinants which could be detected by a variety of immunological techniques. | 1975 | 5338 |
| site-site interactions among insulin receptors. characterization of the negative cooperativity. | by studying the dissociation of 125i-instulin from its receptors in the absence and phe negatively cooperative type for the insulin receptors. in the present study we extend oy purified mouse and rat liver membranes as well as in human circulating monocytes and human cultured lymphocytes demonstrated negative cooperativity that was extraordinarily simn membranes more slowly than it does from its receptors on whole cells. the dissociaty a small percentage of the receptor sites (1 to 5%), are suff ... | 1976 | 5434 |
| use of 300-msec microwave irradiation for enzyme inactivation: a study of effects of sodium pentobarbital on acetylcholine concentration in mouse brain regions. | microwave irradiation of 6 kw at 2450 mhz for 300 msec was sufficient to completely inactivate mouse brain cholinesterase and choline acetyltransferase. after this method of sacrifice, the acetylcholine contents of mouse brain regions, given in nanomoles per gram, were found to be: striatum, 81; medulla-pons, 44; diencephalon-midbrain, 34; hippocampus, 31; cerebral cortex, 26; and cerebellum, 17. sodium pentobarbital caused a dose-dependent increase in whole brain acetylcholine. a maximal increa ... | 1976 | 5594 |
| vinyl chloride dependent mutagensis: effects of liver extracts and free radicals. | the mutagenic effects of vinyl chloride (vc) on salmonella typhimurium strain ta1530 are enhanced by mouse or rat liver extracts. the extracts prepared from mice pretreated either with vinyl chloride or the microsomal enzyme inducer, aroclor 1254, did not produce any greater stimulation of vc-dependent mutagenesis than extracts from untreated animals. these same extracts, however, differed markedly in their capacity to stimulate the mutagenicity of dimethylnitrosamine (dmn), a compound which is ... | 1976 | 5669 |
| regulatory mast cells. i suppressive action of their products on an in vitro primary immune reaction. | products of mast cell degranulation, as well as histamine and serotonin, were added to a mishell and dutton preparation for in vitro primary immunisation (induction of igm antibody formation) to sheep or horse red blood cells. degranulation products were either obatined beforehand by reacting passively sensitised mast cells with the corresponding antigen (unrelated to or identical with the in vitro immunising antigen) or liberated into the culture medium where mast cells actively sensitised to t ... | 1975 | 5941 |
| participation of mouse t- and b-lymphocytes in the graft versus host reaction. | cells of the spleen or lymph nodes of cba mice were transplanted to sublethally irradiated (cbaxc57bl/6)f1 mice; this caused development of the graft-versus-host reaction (gvhr). lymphocytes lost the capacity to realize this reaction after in vitro treatment with specific sera against mouse t- and b-lymphocytes. apparently, development of the gvhr in mice was connected with the cooperative interaction of t- and b-lymphocytes. | 1976 | 8176 |
| biosynthesis of fatty acids in mouse brain mitochondria in the presence of malonyl-coa or acetyl-coa. | incorporation of malonyl-coa or acetyl-coa is studied in mouse brain mitochondrial fatty acids. rupture of mitochondria is necessary ; triton x-100 gives the best result. other detergents or sonication are of lesser efficiency. cofactor requirements have been studied : nadh and nadph have been tested ; atp increases biosynthesis and coa causes an inhibition. two systems of biosynthesis are involved : -- one is a de novo system using malonyl-coa. malonyl-coa alone is incorporated and synthesizes ... | 1976 | 6069 |
| biochemical differentiation of mechanically dissociated mammalian brain in aggregating cell culture. | mouse and rat brain cells were dissociated by a simple mechanical sieving technique and studied in culture for the formation of aggregates and the activities of choline acetyltransferase, acetylcholinesterase, glutamic acid decarboxylase, tyrosine 3-monooxygenase, aromatic l-amino acid decarboxylase, catechol methyltransferase, and monoamine oxidase. cells from fetal and neonatal tissue formed aggregates but not cells from tissue older than two days after birth. the pattern of development of enz ... | 1976 | 6121 |
| genetic and developmental variation in mouse brain tryptophan hydroxylase activity. | | 1976 | 6123 |
| activation of mouse splenic lymphocyte guanylate cyclase by calcium ion. | the guanosine 3',5'-cyclic monophosphate (cgmp) level in the mouse splenic lymphocytes was increased about 2- to 3-fold by concanavalin a. this increase was completely dependent on the presence of ca2+ in the medium. homogenates of mouse splenic lymphocytes contained significant guanylate cyclase ec 4.6.1.2 activity in both the 105,000 x g (60 min) particulate and supernatant fractions and both fractions required mn2+ for full activity. calcium ion (3mm) activated soluble guanylate cyclase 3-fol ... | 1976 | 6445 |
| enzymic basis for cyclic gmp accumulation in degenerative photoreceptor cells of mouse retina. | the activities of guanylate cyclase, guanosine 3', 5'-monophosphate (cyclic gmp) phosphodiesterase and 5'-nucleotidase were measured during postnatal development in retinas of control and c3h/hej mice. in control retina, each of these enzyme activities increases in conjunction with photoreceptor cell differentiation and maturation. in c3h retina, guanylate cyclase and 5-nucleotidase activities increase with photoreceptor cell development and decrease with photoreceptor cell death. however, the a ... | 1976 | 6493 |
| the transport of tetracyclines across the mouse ileum in vitro: the effect of cations and other agents. | the intestinal transfer of different tetracyclines dissolved in calcium- and magnesium-free krebs bicarbonate buffer solution, ph 7.4, was studied using the everted ileum of the mouse. the rates of transfer of chlortetracycline and demethylchlortetracycline were less than those of tetracycline and oxytetracycline, the latter compounds being transferred at the same rate. addition of calcium and magnesium to the buffer greatly reduced the transfer of tetracycline; this inhibition could be antagoni ... | 1976 | 6672 |
| the correlation between antinociceptive activity of narcotics and their antagonists as measured in the mouse tail-flick test and increased synthesis of brain catecholamines. | the effects of several narcotics, narcotic antagonists-analgesics and narcotic antagonists on the synthesis of dopamine and norepinephrine in mouse brain were estimated and related to their activity in the tail-flick test. catecholamine synthesis was estimated by measuring the accumulation of 3h-dopamine and 3h-norepinephrine formed from an injection of 3h-tyrosine. morphine produced dose-related increases in both tail-flick activity and catecholamine synthesis. each of the narcotic analgesics p ... | 1976 | 6793 |
| participation of cyclophosphamide-sensitive t cells in graft-vs-host reactions. | cyclophosphamide (cy)3 is toxic for a population of mouse t cells which is active in graft vs host (gvh) responses. this observation contrasts with previous reports which indicate that treatment of mice with cy regimens, similar to those used in this study, enhances the reactivity of t cells which mediate delayed type hypersensitivity to sheep red blood cells. the gvh-inducing capacity of t cells is not altered by removal of b cells by nylon wool columns. we suggest that cy-sensitive t cells may ... | 1976 | 8571 |
| enhancement of sulphatide metabolism in the hypertrophied kidney of c3h/he mouse with reference to na+, k+-dependent atpase. | sulphatide (cerebroside sulphate) metabolism of c3h/he mouse kidney was investigated in the course of compensatory renal hypertrophy in association with the change of na+,k+-dependent atpase, arylsulfatase a and beta-galactosidase activity. a remarkable increase in 35s incorporation into kidney sulphatide was observed 24 hours and especially 7 days after unilateral nephrectomy. in contrast, no significant alteration of 32p incorporation into major phospholipids such as phosphatidylcholine, phosp ... | 1976 | 6813 |
| avoidance of graft versus host reactions in cured w-anemic mice. | graft-versus-host reactions of parental cells in f1 hybrids were studied with two unrelated inbred strains of mice that differed at the mouse histocompatibility locus. w-anemic f1 recipients were compared with lethally irradiated normal f1 recipients. both sets of recipients were populated by marrow and spleen cell grafts from parental and f1 donors. most w-anemic f1 recipients were cured by parental and f1 cell grafts (except b6 spleen). even after 13 to 18 months, they showed little or no effe ... | 1976 | 7035 |
| cell populations in a renal lesion produced by local injection of xenogeneic spleen cells in cyclophosphamide-treated rats. | the frequency and distribution of donor and host lymphoid cells in different stages of a lesion produced by injecting mouse spleen cells beneath the renal capsules of rats treated 24 h previously with cyclophosphamide have been studied by immunofluorescent staining with species-specific anti-lymphocyte sera. donor cells were predominant in the early stages of the reaction and penetrated the outer part of the renal cortex, but by day 7 when the lesion reached its maximum extent most of the infilt ... | 1975 | 7220 |
| interactions of the lanthanide- and hapten-binding sites in the fv fragment from the myeloma protein mopc 315. | 1. the interactions of lanthanide metals and dinitrophenyl spin-label haptens with the fv fragment of the mouse myeloma protein mopc 315 were investigated by the techniques of fluorescence, e.s.r. (electron spin resonance) and high-resolution n.m.r. (nuclear magnetic resonance). 2. the protein fluorescence of fv fragment at 340nm is quenched by the haptens (fluorescence enhancement, epsilon=0.15) and enhanced by gd(iii) (epsilon=1.14) and other lanthanides. the binding of the haptens studied her ... | 1976 | 7239 |
| dioxane-induced changes in mouse liver microsomal mixed function oxidase system. | | 1976 | 7331 |
| further observations on the properties of serum and tissue guanase from man and some animal species. optimum ph and activation energy in the presence of 8-azaguanine. | the activation energy and the optimum ph of guanine deaminase in man, the rat, guinea pig and mouse were studied using 8-azaguanine as a substrate. the serum guanase in man and in all the animal species studied differs in activation energy from the guanase of the liver. in man, moreover, the serum guanase is also different from the brain and kidney enzyme. in the rat and guinea pig the brain enzyme has thermic activation energy different from the liver and kidney enzyme. the guanase of the serum ... | 1976 | 7449 |