| aggregation of blood platelets by adrenaline and its uptake. | | 1975 | 20 |
| the influence of ph on the interaction of inhibitors with triosephosphate isomerase and determination of the pka of the active-site carboxyl group. | ionization effects on the binding of the potential transition state analogues 2-phosphoglycolate and 2-phosphoglycolohydroxamate appear to be attributable to the changing state of ionization of the ligands themselves, therefore it is unnecessary to postulate the additional involvement of an ionizing residue at the active site of triosephosphate isomerase to explain the influence of changing ph on ki in the neutral range. the binding of the competitive inhibitor inorganic sulfate is insensitive t ... | 1975 | 47 |
| bile acids. xlvii. 12alpha-hydroxylation of precursors of allo bile acids by rabbit liver microsomes. | rabbit liver microsomal preparations fortified with 0.1 mm nadph effectively promote hydroxylation of 3beta-3h- or 24-14callochenodeoxycholic acid or 5alpha,6alpha-3h25alpha-cholestane-3alpha,7alpha-diol to their respective 12alpha-hydroxyl derivatives in yields of about 25 or 65% in 60 min. minor amounts of other products are formed from the diol. the requirements for activity of rabbit liver microsomal 12alpha-hydroxylase resemble those of rat liver microsomes. of a number of enzyme inhibitors ... | 1975 | 65 |
| radioactive labeling and location of specific thiol groups in myosin from fast, slow and cardiac muscles. | 1. based on incorporation of radioactively labeled n-ethylmaleimide, the readily reactive thiol groups of isolated myosin (ec 3.6.1.3) from fast, slow and cardiac muscles could be classified into 3 types. all 3 myosins contain 2 thiol-1, 2 thiol-2 and a variable number of thiol-3 groups per molecule. both thiol-1 and thiol-2 groups which are essential for functioning of the k+-stimulated atpase, are located in the heavy chains in all 3 myosin types. 2. the variation in the incorporation pattern ... | 1975 | 73 |
| characteristics of the dephosphorylated form of phosphorylase purified from rat liver and measurement of its activity in crude liver preparations. | the phosphorylated form of liver glycogen phosphorylase (alpha-1,4-glucan : orthophosphate alpha-glucosyl-transferase, ec 2.4.1.1) (phosphorylase a) is active and easily measured while the dephosphorylated form (phosphorylase b), in contrast to the muscle enzyme, has been reported to be essentially inactive even in the presence of amp. we have purified both forms of phosphorylase from rat liver and studied the characteristics of each. phosphorylase b activity can be measured with our assay condi ... | 1975 | 75 |
| multiple forms of casein kinase from rabbit erythrocytes. | two rabbit erythrocyte casein kinases, gtp:casein kinase i and gtp:casein kinase ii, have been purified 29 000- and 47 000-fold, respectively. studies employing sucrose density gradient centrifugation indicate that kinase i has a molecular weight of about 9.5 - 10(5) (25 s) and kinase ii about 1.4 - 10(6) (32 s). these enzymes can utilize either atp or gtp as the phosphoryl donor. among various protein substrates examined, these kinases catalyze the phosphorylation of casein greater than 50% dep ... | 1975 | 76 |
| kinetic studies and effects of anions on creatine phosphokinase from skeletal muscle of rhesus monkey (macaca mulatta). | a purification procedure for creatine kinase (ec 2.7.3.2) from muscle of the monke35--170 muequiv h+/mg protein per min at 30 degrees c and a yield of approx. 0.5 g/kg muscle. assuming equilibrium kinetics, synergistic binding of substrates at one catalytic site is found for both the forward and back reactions. kinetic constants for the binding of each substrate to the free enzyme and the enzyme-second substrate complex are determined and are compared with those for the enzyme from other species ... | 1975 | 77 |
| alterations in phospholipid-dependent (na+ +k+)-atpase activity due to lipid fluidity. effects of cholesterol and mg2+. | the (na+ +k+)-activated, mg2+-dependent atpase from rabbit kidney outer medulla was prepared in a partially inactivated, soluble form depleted of endogenous phospholipids, using deoxycholate. this preparation was reactivated 10 to 50-fold by sonicated liposomes of phosphatidylserine, but not by non-sonicated phosphatidylserine liposomes or sonicated phosphatidylcholine liposomes. the reconstituted enzyme resembled native membrane preparations of (na+ +k+)-atpase in its ph optimum being around 7. ... | 1975 | 90 |
| effect of carbidine on conditioned defense reflexes. | chronic experiments were conducted on rats and rabbits; a study was made of the effect of carbidine on the conditioned defence reflexes in stimulation of the mesencephalic part of the reticular formation. carbidine prevented the depression of the conditioned defence reflexes caused by stimulation of the mesencephalic portion of the reticular formation. this pointed to its depressive influence on the mentioned structures, and was confirmed by experiments on rabbits in recording changes in biocurr ... | 1975 | 105 |
| proceedings: the mechanism of "adrenaline reversal" in the anaesthetized cat and rabbit. | | 1975 | 116 |
| the prevention of autolysis of stored cornea using steroid as a lysosome membrane stabilizer. | many eyes donated for use in corneal grafting are rejected because of signs of autolysis in the donor material. the purpose of this experimental study was to determine whether hydrocortisone acting as a lysosome membrane stabilizer could prevent or retard autolysis of the corneas under storage, and if so, what was the most efficacious concentration. different groups of rabbit corneas were placed in saline as controls or in varying concentrations of hydrocortisone (10(-10) m to 10(-4) m at ph 7.4 ... | 1975 | 133 |
| basic phospholipase of naja nigricollis venom. | it is confirmed that n. nigricollis venom contains several phospholipases one of these is a basic phospholipase a. this enzyme is toxic for mice when injected intravenously. in vitro it reacts on egg yolk lecithin producing lysolecithin and prevents the phenomenon of blood clotting. an immunological identity has been established between this basic phospholipase and two acidic phospholipases present in the same venom. | 1975 | 151 |
| the metabolic fate of securinine. | | 1975 | 219 |
| the interaction of so2 with proteins. | | 1975 | 228 |
| studies on the mechanism of the changes in serum and liver gamma-glutamyl transpeptidase activity. i. experimental extrahepatic cholestasis in rabbits. | serum, liver and renal gamma-glutamyl transpeptidase (ggt) activities were studied in four groups of rabbits: controls, rabbits with obstructive extrahepatic cholestasis, rabbits with obstructive anuria, and animals with combined obstructive extrahepatic cholestasis and obstructive anuria. serum ggt was essentially increased in rabbits with obstructive extrahepatic cholestasis, showing peak values in the combined cholestasis + obstructive anuria group, and practically normal values in animals wi ... | 1976 | 232 |
| studies on the mechanism of the changes in serum and liver gamma-glutamyl transpeptidase activity. ii. experimental hexachlorobenzene porphyria in rabbits. | the mechanism responsible for the changes in serum and liver gamma-glutamyl transpeptidase (gamma-gt) activity was studied in a model of experimental hexachlorobenzene porphyria in rabbits. porphyria followed the administration of hexachlorobenzene in doses of 280 mumol - kg-1 body weight, which were given daily through a gastric tube over a 20-day period. serum gamma-gt activity and the activities of the lysosomal enzymes beta-n-acetylglucosaminidase and alpha-mannosidase were increased, wherea ... | 1976 | 233 |
| monoamine oxidase (xxxvi). characteristics of benzylamine oxidase in the dog serum. | enzymic properties of monoamine oxidase (mao) in dog serum were studied and the following results were obtained. some of enzymic properties of mao in dog serum differed from that of mitochondrial mao. when dog serum was fractionated by ammonium sulfate, proteins were concentrated in two fractions, such as 25 approximately 33% and 67 approximately 80% of saturated ammonium sulfate fraction, while mao activity was concentrated in 40 approximately 50% of saturated ammonium sulfate fraction. the rea ... | 1975 | 280 |
| combined use of bucolome and pyrazolone derivatives (1). pharmacological activities and blood concentration. | a combination of two or more drugs may exert a drug-drug interaction, in which case the effect can be potentiated or antagonized. such synergistic effects are well known in the case of pyrabital (barbital + aminopyrine) or irgapyrine (phenylbutazone + aminopyrine). bucolome (bcp), a non-steroidal anti-inflammatory agent, has the chemical structure of a barbiturate and also resembles the formula of pheylbutazone. thus the influence of bcp combination on the pharmacological activities of various p ... | 1975 | 281 |
| isoelectric distribution of specifically purified rabbit antibody against 4-azo-naphthalene-1-sulfonate. | | 1975 | 323 |
| studies of hyperimmune restricted and partially restricted anti-pneumococcal polysaccharide antibodies from allotype-defined pedigreed rabbits--iv. amino-terminal light chain sequence analyses of restricted homozygous b4 rabbit anti-siii and sviii antibodies from partially inbred rabbits. | | 1975 | 324 |
| effect of pneumococci on blood clotting, platelets, and polymorphonuclear leukocytes. | infections due to streptococcus pneumoniae and products from the organism have been associated with alterations in blood clotting and function of platelets. pneumococci and pneumococcal polysaccharide shortened the clotting times of whole blood, platelet-rich plasma (prp), and platelet-poor plasma (ppp) in vitro. clotting times of ppp and prp from c6-deficient animals were likewise decreased. the bacteria had no effect on the one-stage prothrombin time or the partial thromboplastin time when th ... | 1975 | 331 |
| phenomenon of hot-cold hemolysis: chelator-induced lysis of sphingomyelinase-treated erythrocytes. | staphylococcus aureus produces a phospholipase c specific for sphingomyelin (beta-hemolysin). erythrocytes with approximately 50% sphingomyelin in their membranes, e.g., from sheep, have been shown to have up to 60% of this phospholipid hydrolyzed by this enzyme at 37 c in isotonic buffered saline without hemolysis. cooling of sphingomyelinase c-treated erythrocytes to 4 c causes complete lysis of the cells, a phenomenon known as hot-cold hemolysis. the addition of ethylenediaminetetraacetate (e ... | 1975 | 333 |
| computer evaluation of the effect of urecholine on the spontaneous activity of smooth muscle from the urinary bladder of the rabbit. | the spontaneous activity exhibited in vitro by smooth muscle strips from the urinary bladder of the rabbit under the influence of urecholine in concentrations from 0.3 to 30 mum has been characterized in terms of three parameters: mean tension, frequency of contraction, and contractile deviation, all obtained by computer an alysis. while mean tension and frequency monotonically increased with concentration, the contractile deviation rose to a peak in the vicinity of 3.0 to 6.0 mum and thereafter ... | 1975 | 342 |
| stability of myofibrillar edta-atpase in rabbit psoas fiber bundles. | | 1975 | 435 |
| hydrolysis of the hen egg vitelline membrane by cock sperm acrosin and other enzymes. | a technique utilizing pregnant mare's serum gonadotropin and human chorionic gonadotropin treatment of hens (gallus domesticus), followed by manual ovulation of the excised follicles, was developed to obtain a large number of mature ova. the intact ova were used to test whether acrosin, partially purified from the spermatozoa of the cock (gallus domesticus), partially purified rabbit testicular acrosin and commercial preparations of several hydrolytic enzymes could dissolve the inner vitelline m ... | 1975 | 454 |
| effect of cross-reinnervation on physiological parameters and on properties of myosin and sarcoplasmic reticulum of fast and slow muscles of the rabbit. | cross-reinnvervation of fast (extensor digitorum longus) and slow (soleus) twitch muscles of the rabbit showed essentially complete fast to slow and slow to fast conversion, respectively, 11-12 mo after surgery with respect to a number of physiological parameters including intrinsic shortening, velocity, and isometric twitch time to peak. there was pronounced bu incomplete biochemical conversion as judged by ca2+ uptake by sarcoplasmic reticulum, myosin atpase, alkali lability, and light chain c ... | 1975 | 461 |
| cation permeation mechanisms and cation selectivity in "tight junctions" of gallbladder epithelium. | | 1975 | 587 |
| histamine receptors in the vasculature of the rabbit ear. | histamine has a dual action on the isolated perfused ear preparation of the rabbit. the amine induced a dose-dependent rise in perfusion pressure when the preparation was perfused with krebs' solution. this pressor response was reversed to a depressor effect when meypramine was added to the perfusion fluid. this depressor effect of the amine was also dose-related. metiamide competitively inhibited the depressor effect of histamine. prior treatment of the ear vessels with metiamide alone caused a ... | 1975 | 626 |
| relative pre- and postsynaptic potencies of alpha-adrenoceptor agonists in the rabbit pulmonary artery. | the rabbit pulmonary artery contains postsynaptic alpha-adrenoceptors which meidate smooth muscle contraction; its noradrenergic nerves contain presynaptic alpha-adrenoceptors which mediate inhibition of the release of the transmitter evoked by nerve impulses. dose-response curves for the pre- and postsynaptic effects of eight alpha-receptor agonists were determined on superfused strips of the artery in the presence of cocaine, corticosterone and propranolo. 1. according to the concentrations wh ... | 1975 | 627 |
| a study of the central effects of sympathomimetic drugs: eeg and behavioural investigations on clonidine and naphazoline. | | 1975 | 637 |
| the influence of cortisol on the enzymes of fatty acid synthesis in developing mammalian lung and brain. | administration of cortisol to fetal rabbits resulted in a 42% inhibition of pulmonary de novo fatty acid synthesis from acetyl coenzyme a (coa) (p = less than 0.025). this was associated with inhibition of acetyl-coa carboxylase (ec. 6.4.1.2.) activity (p = less than 0.01) and a tendency towards decreased activity of fatty acid synthetase. there was no effect on pulmonary microsomal fatty acid elongation activity. light and electron microscopic examination of the apex of the right lung of contro ... | 1975 | 648 |
| the relation between myosin adenosinetriphosphatase activity and inactivation of myosin under alkaline conditions of heart muscles in mammals of different size. | atpase activity of myosin in the heart muscle of the mouse, rat, guinea-pig, rabbit and pig was studied at neutral ph and under mild alkaline conditions. at neutral ph the atpase activity of myosin is inversely related to body size of the animal species. the decrease of atpase activity of myosin after alkaline preincubation depends on the degree of atpase activity of intact myosin, i.e. myosin from the heart of the mouse exhibits high atpase activity ae same relationship was found, when comparin ... | 1975 | 651 |
| effect of sex hormones preparations on some indices of liver function under experimental conditions. | | 1975 | 659 |
| effect of two weeks' treatment with thioridazine, chlorpromazine, sulpiride and bromazepam, alone or in combination with alcohol, on learning and memory in man. | forty paid healthy male students participated in two subacute experiments of 6 weeks each. in the first trial 20 of them received bromazepam, thioridazine, and placebo double blind cross over for 2 weeks each, and in the second trial the active agents administered to the other 20 participants were chlorpromazine and sulpiride. the tests used were paired associate learning with nonsense syllables and digit memory span. before testing the subjects took either an alcoholic or a nonalcoholic bitter ... | 1975 | 710 |
| the renin-angiotensin system and drinking behavior. | | 1975 | 732 |
| the role of mg and ca ions in muscle contraction (author's transl). | | 1975 | 739 |
| premise for the pathogenetic definition of toxinfectious and endotoxic shock states. | | 1975 | 774 |
| effect of lysosomal cationic proteins from polymorphonuclear leukocytes upon the fibrinogen and fibrinolysis system. | | 1975 | 811 |
| effects of oxygen saturation and pco2 on brain uptake of glucose analogues in rabbits. | the effect of oxygen saturation and pco2 on brain uptake of glucose analogues was studied in rabbits. using a modified oldendorf technique, 14c-labeled glucose analogues with a 3h2o reference standard were introduced into the cerebral circulation via the common carotid artery, and the radioactivity of the ipsilateral cerebral cortex was counted and expressed in terms of a brain uptake index (bui). severe hypoxia (oxygen saturation less than or equal to 18%) resulted in approximately a 40% decrea ... | 1975 | 821 |
| dopamine-containing neurons of the substantia nigra and their terminals in the neostriatum. | tne ultrastructural and fluorescence histochemical characteristics of the mature rabbit substantia nigra and neostriatum have been reviewed as a frame of reference for the developmental study. biochemical investigations were reported on neostriatal dopamine concentrations and the relative uptake and accumulation of 3h-dopamine by this tissue from fetal to adult stages, to provide quantitative data for correlation with the fluorexcence information. the development of the neurons of the substantia ... | 1975 | 827 |
| determination of optimal conditions for the electron-cytochemical detection of atpase activity in isolated nuclei. | the optimal conditions are selected for electron-cytochemical detection of the atpase activity in nuclei of the skeletal muscles of rabbits and nuclei of vicia faba l. meristem. it is shown that the previous fixation of nuclei in the rabbit skeletal muscle for 10 min in a mixture of the buffer solutions of 4% glutaric dialdehyde and 4% neutral formalin (1:1) causes a decrease in their atpase activity by 78% in the medium containing mg2+ and by 34% - in the medium containing ca2+; in nuclei of ho ... | 1975 | 836 |
| experimental ulcer. | | 1975 | 855 |
| the cathode bound group antigen of dysentery-provoking escherichieae (author's transl). | antigens from disrupted cells of dysentery-provoking and of non-enteropathogenic escherichieae were submitted to immunoelectrophoresis on cellulose acetate stripes at ph 8.0. among 6 immune sera produced for this purpose by immunizing rabbits against desintegrated dysentery bacteria, only one contained a precipitine reacting with an antigen similar to the "generic antigen" of belaya. this - at ph 8.0 - cathode-bound group antigen (kga) could not only be found in virulent but also in 5 attenuated ... | 1975 | 873 |
| further characterization of the association of glyceraldehyde-3-phosphate dehydrogenase with reticulocyte membranes. | 1. the behaviour and properties of membrane-bound gapdh of rabbit reticulocytes were investigated. 2. the bound gapdh is more resistant to inactivation by kcl than the soluble enzyme (allotopy). 3. the bound enzyme is released by electrolytes. this effect does not only depend on the ionic strength but additionally on the kind of ions, ph-value and protein concentration. 4. a comparison of the releasing effect of nad analogues shows the necessity of the 5'-amp moiety in the structure of the effec ... | 1975 | 880 |
| effect of the vagus nerve on isolated rabbit atria in ganglionic blockade due to hexamethonium. | by quantitative stimulation of the vagus nerves of isolated rabbit atria frequency-response relations were obtained for both the electrotropic effect (reduction of the area of the monophasic action potential) and the inotropic response. an addition of hexamethonium in a final concentration of 10(-5) g/ml resulted in a diminution of vagal effectivity in the range of lower and medium frequencies of stimulation, and was connected with a shift of the frequency-response characteristic to the right. a ... | 1975 | 881 |
| sex as a factor in metabolism, toxicity, and efficacy of pharmacodynamic and chemotherapeutic agents. | | 1975 | 901 |
| effect of amphetamine-type psychostimulants on brain metabolism. | | 1975 | 902 |
| ecs, intracellular ph, and electrolytes of cardiac and skeletal muscle. | the extracellular space (ecs) of muscle from each ventricle of the heart (rv and lv), the atria, diaphragm, and quadriceps was estimated in the anesthetized rabbit from the distribution volumes of 14cinsulin, 14csucrose, 51credta, and c1--. whole-tissue electrolytes were measured and intracellular electrolytes calculated. the ecs of the tissues varied, increasing in the order quadriceps less than lv less than rv less than atria. the volume of distribution of 14cinulin was always less than that o ... | 1975 | 910 |
| intracellular ph and k+ of cardiac and skeletal muscle in acidosis and alkalosis. | the effects of a metabolic and respiratory acidosis and alkalosis on intracellular ph (phi) and k+ have been compared in cardiac and skeletal muscle from the anesthetized rabbit. the extracellular space and phi were calculated from the distribution volumes of 51cr edta and 14cdmo, respectively. when phe was varied by altering pco2, the slope of the line relating phi to the extracellular ph (phe) was greater (p less than 0.05--0.001) than that obtained during metabolic changes of phe in right and ... | 1975 | 911 |
| separation of low molecular weight rna by polyacrylamide gel electrophoresis. | | 1975 | 925 |
| ascorbic acid and biological systems. ascorbic acid and electron transport. | | 1975 | 941 |
| evidence of an essential histidine residue in rabbit liver aryl sulfatase a. | | 1975 | 958 |
| carbohydrate compositions of the rabbit plasminogen isozymes. | | 1975 | 967 |
| hyperthermia in the rabbit. ii. studies on influencing respiration with exogenic hyperthermia. | rectal temperature, respiratory rate, arterial and venous ph and arterial and venous pco2 were recorded at intervals of 15-30 minutes in female broiler rabbits exposed an environmental temperature of 35 degrees c, until they died. respiratory rate and blood ph rose, and pco2 fell, until a rectal temperature of 42 degrees c was reached. upon further increase in rectal temperature the respiratory rate and ph began to fall, while pco2 began to rise. the rabbits died when rectal temperature reached ... | 1975 | 977 |
| acidic antiinflammatory agents--correlations of some physical, pharmacological and clinical data. | fifteen acidic antiinflammatory agents, for which some clinical data have previously been published, have been examined for their potency in the carrageenan-induced rat foot edema test, and for their acidity (pka) and partition coefficients. published serum half-life data and daily clinical (anti-arthritic) dose have been tabulated for these drugs and correlations between these various parameters are discussed. the rat foot edema carrageenan test has proved to be a fairly reliable predictor of c ... | 1975 | 1042 |
| flavoxate and 3-methylflavone-8-carboxylic acid. assay methods in blood and urine, plasma-red cells repartition and stability. | the following assay methods for pharmacokinetic studies on flavoxate (f) and on its main metabolite, i.e. 3-methylflavone-8-carboxylic acid (a), are described. 1. spectrophotometry for the assay of f and of a in plasma, 2. tlc-spectrodensitometry and glc for the assay of a in urine after acid hydrolysis, 3. tlc-spectrodensitometry for determining the f : a ratio in plasma or in urine. it was found that f hydrolyzes into a. this process depends on the ph and on the medium. in water, at ph 5.0, f ... | 1975 | 1048 |
| on the pharmacology of 9,10-dihydro-10-(1-methyl-4-piperidylidene)-9-anthrol (wa 335), a histamine and serotonin antagonist (author's transl). | the substance 9,10-dihydro-10-(1-methyl-4-piperidylidene)-9-anthrol (wa 335) was examined for its antagonistic effects against histamine and serotonin, for its atropine-like properties as well as for a series of other qualities in comparison with cyproheptadine and pimethixene. the anti-histamine and anti-serotonin activities of compound wa 335 on the smooth muscle and the capillary do not only exceed that of cyproheptadine but also that of pimethixene. wa 335 shows an extremely strong binding t ... | 1975 | 1049 |
| central action of wa-335-bs, a substance with peripheral antiserotonin and antihistaminic activity. | in rats and mice the serotonin and histamine antagonistic drug 9,10-dihydro-10-(1-methyl-4-piperidylidene)-9-anthrol (wa 335-bs) caused stronger central sedative effects than did cyproheptadine. wa 335-bs also displayed stronger activity against reserpine- and central tremorine-induced effects than did cyproheptadine and it slightly enhanced d-amphetamine-induced effects: therefore it may have antidepressant properties. wa 335-bs proved to be very effective against isolation-induced aggression i ... | 1975 | 1050 |
| multiple forms of rabbit muscle phosphofructokinase revealed by means of specific elution. | the modified procedure for rabbit skeletal muscle phosphofructokinase (pfk) purification is worked out utioizing the method of specific elution from deae-cellulose in 0.1 m tris-edta-phosphate ph 8.0 with 10 mm citrate. by the latter procedure pfk can be resolved into fractions a, b and c which are eluted specifically, with 0.3 m buffer and with 1.5 m nacl respectively. rechromatography of each fraction reveals their interconvertibility. the preparations are characterized by disc electrophoresis ... | 1975 | 1113 |
| the use of radioactive microspheres to compare the effects of hydralazine, guanethidine and sk & f 24260 on the redistribution of cardiac output in anaesthetized rabbits. | 1 the use of radioactive microspheres is described for the measurement of cardiac output in anaesthetized rabbits and its redistribution after the administration of drugs which lower blood pressure. 2 hydralazine increased peripheral vascular conductance by 123%. the vascular beds in which it had most effect were those of the carcass (mainly muscle) and the kidneys. 3 sk&f 24260, (1,4 dihydro-2, 6-dimethyl-4(2-trifluoremethylpheny)-3,5,-pyridinedicarboxylic acid diethyl ester), had similar vasoc ... | 1975 | 1134 |
| further studies of metyrapone effects upon anilide hydroxylation. | the enhancing effect of metyrapone upon the p-hydroxylation of acetanilide has been confirmed with the use of a new gas-chromatographic method for the determination of acetaminophen. this effect has been shown not to be due to inhibition of hydrolysis of acetaminophen or interference with its determination, or to preferential formation of other phenolic metabolites. this effect of metyrapone is remarkably substrate-specific: phenol formation from the homologues of acetanilide, formanilide and pr ... | 1975 | 1226 |
| uptake and disposition of aldrin and dieldrin by isolated perfused rabbit lung. | the uptake, metabolism, and release of aldrin and dieldrin by the lungs were studied by use of isolated perfused rabbit lungs that were artificially ventilated and perfused through the pulmonary artery. both recirculating and single-pass experiments were conducted using an artificial medium as perfusate. aldrin accumulated in the lung from the perfusate through two distinct phases of uptake: a rapid phase involving simple diffusion and nonspecific binding and a slower phase representing its meta ... | 1975 | 1232 |
| spin-labelled phosphofructokinase. a simple and direct approach to the study of allosteric equilibria under near-physiological conditions. | rabbit muscle phosphofructokinase, spin-labelled at its most reactive thiol group, has an electron spin resonance spectrum which is very sensitive to the binding of substrates and allosteric effectors. the spectral changes have been interpreted in terms of a concerted allosteric transition between two conformational states with non-exclusive binding of effectors. on this basis mgatp, fructose 6-phosphate plus atp, and nh+4ions behave as potent positive effectors, inorganic phosphate, sulphate, a ... | 1975 | 1264 |
| hemodynamics and its regulation in old age. | | 1975 | 1284 |
| possible structural-functional organization of the system of local cerebral blood flow regulation. | rcbf under normal conditions in the rabbit, cat, and monkey brain was found to have a spontaneous periodicity while rcbf responses to afferent flicker stimulation usually revealed a double-phasic fluctuative pattern. this suggests that the rcbf regulatory system consists of not less than two regulatory chains with different time constants, and a feedback. the data on cerebral vascular responses to microapplication of mcsf solutions with various ph, potassium and catecholamines concentrations, su ... | 1975 | 1291 |
| a micro-method for quantitative determination of acylneuraminic acids from erythrocyte membranes. | a micro-method is presented which enables the fast and exact determination of acid-hydrolyzed acylneuraminic acids in erythrocyte membranes. erythrocytes from 1 ml of human and rabbit blood containing acd buffer are, washed and hemolyzed on millipore filters of pore size 1.2 mu. acylneuraminic acids are released from the erythrocyte membranes still on the filters under the optimal conditions of 0.1 n hcl at 80 degrees c for 50 min. a prerequisite for the determination of the true amount of acyln ... | 1975 | 1335 |
| the effect of acid treatment upon the susceptibility of rabbit igg to proteolytic cleavage with various enzymes. | | 1975 | 1342 |
| interaction of adrenergic antagonists with prostaglandin e2 and tetrahydrocannabinol in the eye. | both alpha- and beta-adrenergic antagonists have been utilized in an atempt to discern the site of action of prostaglandin (pg) and tetrahydrocannabinol (thc) in the eye. both alpha- and beta-adrenergic antagonists (alpha-antagonists, phentolamine and phenoxybenzamine; beta-antagonists, propranolol and sotalol) cuased a dose-dependent reduction in intraocular pressure and blood pressure and increased total outflow facility. the results are consistent with the concept that both alpha- and beta-ad ... | 1976 | 1356 |
| induction of corneal graft rejection by passive cell transfer. | an experimental model is presented demonstrating that penetrating corneal grafts in the rabbit may be rejected by passive transfer into the anterior chamber of specifically sensitized lymphoid cells. destruction of histo-incompatible corneal endothelium is always marked by the formation of focal pock-like areas of damage in this system, rather than by the typical moving line of rejecting endothelium usually seen in spontaneous graft rejection. where the transferred lymphoid cells are compatible ... | 1976 | 1357 |
| monomeric purine nucleoside phosphorylase from rabbit liver. purification and characterization. | rabbit liver purine nucleoside phosphorylase (purine nucleoside: orthophosphate ribosyltransferase ec 2.4.2.1.) was purified to homogeneity by column chromatography and ammonium sulfate fractionation. homogeneity was established by disc gel electrophoresis in presence and absence of sodium dodecyl sulfate, and isoelectric focusing. molecular weights of 46,000 and 39,000 were determined, respectively, by gel filtration and by sodium dodecyl sulfate-polyacrylamide disc gel electrophoresis. product ... | 1976 | 1390 |
| the effect of neonatal rat graft-vs-host disease (gvhd) on fc receptor lymphocytes. | the level of fc receptor rosette-forming lymphocytes (fc-rfl) was examined in spleen and lymph node cell suspension from neonatal da and bn rats inoculated within 24 hr of birth with either allogeneic l (experimental) or syngeneic (control) lymphoid cells. in addition, these levels were compared to fetal and neonatal animals that received no injection. the indicator cells (ea) were sheep erythrocytes sensitized with one-half concentration of the highest dilution of rabbit anti-sheep erythrocyte ... | 1976 | 1444 |
| the polysaccharide capsule of bacteroides fragilis subspecies fragilis: immunochemical and morphologic definition. | a large-molecular-weight capsular polysaccharide was isolated from strains of bacteroides fragilis subspecies fragilis. by means of electron microscopy and staining with ruthenium red, the thick polysaccharide capsule was also visualized. with use of a radioactive antigen-binding assay, antibody to this capsular polysaccharide was demonstrated in antisera prepared in rabbits to each of eight strains of b. fragilis fragilis. antibody of similar specificity was not found in antisera prepared to ba ... | 1976 | 1451 |
| the formation of cdp-diglyceride by isolated neuronal nuclei. | | 1975 | 1472 |
| the in vitro adsorption of drugs from horse serum onto carbon coated with an acrylic hydrogel. | in vitro studies have shown that uncoated carbon and carbon coated with an acrylic hydrogel are capable of adsorbing drugs from horse serum at 37 degrees. increase in the coating weight from 2 to 4% decreased the rate of adsorption but not the total capacity. in vivo data supports the concept of carbon haemoperfusion for use in the treatment of drug overdose. | 1975 | 1486 |
| failure of pyruvate to counteract isoniazid toxicity in rabbits. | | 1975 | 1507 |
| quantitative analysis of tribromsalan in blood and urine. | tribromsalan can be quantitatively measured in whole blood and urine by a technique involving extraction with ethyl acetate, treatment with silica gel, separation by tlc, and quantitative measurement by fluorescent spectrophotometry. this method has a sensitivity down to 125 ng (25 ppb in 5.0 ml of sample) of free tribromsalan and shows an average 90% recovery of tribromsalan in blood and urine with standard deviations of 9.7 and 7.4%, respectively. | 1975 | 1513 |
| renal chromium accumulation and its relationship to chromium-induced nephrotoxicity. | this report is an attempt to study the renal handling of chromium under in vitro conditions and to relate this to the actions of the ion in the production of nephrotoxicity. renal slice techniques were employed in these studies and were used to examine the effects of chromium on various renal transport processes. in addition, the accumulation of chromium by the renal tissue has also been studied. marked accumulation by renal cortical slices of the rat was observed when 51cr-labeled chromate or d ... | 1976 | 1551 |
| stimulators of beta-adrenergic structures in treatment of ischemic heart disease. | the beneficial effect of stimulators of beta-adrenergic structures (myophedrin on the haemodynamics and the inotropic function of the myocardium was demonstrated experimentally (in 12 rabbits) and clinically (in 53 patients with ischaemic heart disease). a positive effect of the treatment was noted in 45.5% of those patients in whom ischaemic heart disease manifested itself in angina decubitus and angina of effort. | 1975 | 1568 |
| nonachlasine--a new drug for treatment of ischemic heart disease. | data on the pharmacology of a new antianginal drug--nonachlasine--are presented. nonachlasine was found to increase the blood flow intensively and for long periods of time, increasing the oxygen reserve of the myocardium, thus increasing the cardiac output and the contractile function of the heart. the prevailing action of nonachlasine on the blood supply and the function of the myocardium seems to be the result of several mechanisms: decreasing resistance of the coronaries due to the activation ... | 1975 | 1569 |
| the influence of isoglaucon on the hydrodynamics and haemodynamics of the eye (author's transl). | by animal experiments and clinically we could prove that isoglaucon instillation caused the decrease in iop due to both increase in outflow and reduction of humor secretion; the iop decrease and changes in hydrodynamics being manifested more markedly in the fellow-eye. as reoophthalmography demonstrated "isoglaucon" produced lumen narrowing in the anterior portion of the uveal tract. "isoglaucon" was prescribed to 20 patients having open-angle-glaucoma, two hours after instillation the 23 decrea ... | 1975 | 1573 |
| mosquito-borne arboviruses in arctic america. | mosquito-borne arboviruses are prevalent throughout subarctic regions of canada and alaska, principally in the boreal forest extending between latitudes 53 and 66 degrees n, but they have been identified in tundra regions as far north as 70 degrees n. all mosquito-borne agents have been bunyaviruses, comprising principally the snowshoe hare subtype of california encephalitis (ce) virus, but also northway virus. mosquito vectors comprise several aedes species and culiseta inornata, all of which h ... | 1975 | 1602 |
| treatment of ovarian hyperstimulation syndrome by antihistamine. | ovarian hyperstimulation was produced by human menopausal gonadotropin and chorionic gonadotropin in rabbits. a more rapid regression of the hyperstimualted ovaries was observed in an antihistamine-treated group than in a control group. the difference in regression was found to be statistically significant. the possibility of treating the ovarian hyperstimulation syndrome by antihistamine is cited. | 1976 | 1697 |
| h+ transport and membrane-bound hco - 3 atpase in salivary duct epithelium. | an atpase stimulated by hco - ions and other oxybases and inhibited by scn- has been found in main excretory duct of rat submaxillary gland, a tissue, capable of actively secreting hco - 3 ions. no such atpase was found in the rabbit duct, which normally does not secrete hco - 3. the hco - 3 atpase was localized in the plasma membrane fraction of the homogenate, as evidenced by the marker 5'-nucleotidase. the activities of the hco - 3 atpase increased in metabolic alkalosis and decreased in met ... | 1975 | 1708 |
| the influence of 5-hydroxytryptamine and some beta-adrenolytic compounds on adenylylcyclase in rabbit brain in vitro. | | 1975 | 1712 |
| properties of a toxin from the sea anemone stoichacis helianthus, including specific binding to sphingomyelin. | stoichactis helianthus toxin, a protein derived presumably from the nematocysts, was purified to homogeneity. it has a molecular weight of about 16,000, an isoelectric ph of 9.8, and it contains approximately 3.7% carbohydrate. it is powerfully hemolytic for erythrocytes derived from a variety of animal species, those of the cat being the most sensitive and those of the guinea pig the most resistant. the toxin is lytic also for rabbit blood platelets, and it destroys cultured fibroblasts but is ... | 1976 | 1757 |
| comparison of beta-adrenoceptor blocking properties of sotalol, oxprenolol, propranolol and pindolol on rabbit intestinal smooth muscle. | adrenergic agonists produced a characteristic and definite decrease in the amplitude of spontaneous contractions and tone of the isolated rabbit jejunum. effect of phenylephrine was abolished either by phenoxybenzamine or phentolamine. relaxation induced by epinephrine and by norepinephrine was inhibited after combined treatment with phentolamine and propranolol. phentolamine alone diminished the response to epinephrine and to norepinephrine, but the diminution for epinephrine was greater, indic ... | 1975 | 1802 |
| dna-rna hybridization. | interest in nucleic acid hybridization stems mainly from its great power as a tool in biological research. it is used in several quite distinct ways. because of the high degree of specificity that they show, hybridization techniques can be used to measure the amount of one specific sequence within a very heterogeneous mixture of sequences. measurements of 1/10(6)-10(7) have been recorded. in extension of this, various properties of a specific sequence can often be studied. secondly, because the ... | 1975 | 1811 |
| construction and evaluation of a simple membrane oxygenator for small organ perfusion. | a simple membrane oxygenator for isolated organ perfusion is described. the membrane employed consisted of an ordinary silicone rubber tubing, 2 mm internal diameter, 0.3 mm wall thickness, the length of the tubing varying according to the required gas transfer. when describing the capacity of the oxygenator, it was found that the maximum gas transfer rate per unit membrane surface was an inadequate measure, since this would vary with both flow rate through the oxygenator and the gas binding cap ... | 1975 | 1854 |
| specific effects of neurotransmitter antagonists on ganglion cells in rabbit retina. | directionally sensitive ganglion cells in rabbit retina lose their directional sensitivity when picrotoxin, an antagonist of the inhibitory neurotransmitter gamma-aminobutyric acid, is infused into the retinal blood supply. strychnine, an antagonist of glycine, does not produce this effect. other receptive field types are affected by strychnine but not picrotoxin. inhibitory transmitters therefore have specific functions in information processing in the retina. | 1976 | 1857 |
| chronic anemia, wound healing, and red cell 2,3-diphosphoglycerate. | relationships between various types of chronic anemia, wound healing, and red cell 2,3-diphosphoglycerate (2,3 dpg) were examined in rabbits. wound tensile strength and energy absorption were not affected by chronic iron-deficiency anemia, the chronic hemolytic anemia caused by intravenous water infusion nor by chronic hemolytic anemia caused by intravenous water infusion nor by chronic phenylhydrazine-induced anemia. red cell 2,3 dpg levels were increased in the anemia of iron deficiency and we ... | 1976 | 1866 |
| fluorocarbons and general metabolism in the rat, rabbit, and dog. | | 1975 | 1870 |
| the mechanism of action and treatment of palytoxin poisoning. | | 1975 | 1871 |
| change of cellular composition of blood and erythropoietic activity of blood serum under the effect of constant magnetic field in high altitude (experimental study). | | 1975 | 1917 |
| hypoxemic hypoxia produced by intravenous controlled infusion of co2 in the rabbit. | | 1975 | 1969 |
| classification and biological distribution of histamine receptor sub-types. | the distribution and classification of histamine receptors in mammalian and avian tissues have been summarized in tables 1-4. it is evident that histamine receptors are present on a number of morphologically distinct cell types and the proportion of cells bearing h1- and h2-receptors varies not only with the species but also with the cell source. the pharmacological receptors mediating mepyramine-sensitive histamine responses have been defined as h1-receptors. receptors mediating mepyramine-resi ... | 1975 | 1979 |
| corneal infections (author's transl). | | 1975 | 1981 |
| the micro-organisms of tsetse flies. | micro-organisms from tsetse fly mycetomes were maintained in culture, where they were more pleomorphic than in the mycetomes, but were in some cases very similar to those observed in ovaries by other authors. agglutination tests on the cultured forms indicated in affinity to rickettsia. they were sensitive to antibiotics introduced by feeding flies on hosts treated with ampicillin; this reduced the longevity and fecundity of the tsetse flies and appeared to disturb normal digestion of bloodmeals ... | 1975 | 1986 |
| effect of adsorbents on igm and igg measles antibodies. | sera from rabbits immunized with l-16 measles virus absorded with monkey blood cells; kaolin and blood cells; and mncl2 and heparin were examined in haemagglutination inhibition (hi) and neutralization tests. kaolin and mncl2 adsorbed primarily hi igm antibodies from the early immunization period. the adsorbents used had no influence on hi and neutralization igg antibodies. human convalescent serum gave similar results, i.e. only igg antibodies were found and they were not affected by kaolin and ... | 1975 | 1989 |
| serological differences between some isolates of bean yellow mosaic virus. | the degree of serological relatedness of three isolates of bean yellow mosaic virus (bymv) was studied. one common antigenic group (m) was demonstrated in all 3 isolates. in addition, another antigenic group (a) was demonstrated in the isolate fvm1. this group was absent from the isolates tpm3 and tpm11, in which the antigenic group n was found. antisera to the 3 isolates contained the corresponding antibody groups. the antibody group (m) shared by all 3 bymv isolates was present in the fvm1 ant ... | 1975 | 1992 |
| effect of ph on ionic exchange and function in rat and rabbit myocardium. | the effects of ph variation on ionic exchange and mechanical function were studied in the arterially perfused rat and rabbit septa. the ph and pco2 of the control perfusate were 7.40 and 39 mmhg, respectively. in the rabbit septum a metabolic acidosis (ph equals 6.82, pco2 equals 39 mmhg) caused a loss of 16% of control tension in 12 min. na+ and k+ exchange were unaltered. a comparable respiratory acidosis (ph equals 6.81, pco2 equals 159 mmhg) caused a 51% loss of tension in 2 min. na+ exchang ... | 1975 | 2014 |