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comparison of antimicrobial resistant escherichia coli in wild and captive japanese serows.the fecal escherichia coli isolated from wild japanese serows living in mountainous areas away from humans and those from captive serows kept in human areas were examined for antimicrobial resistance and the possession of transferable r plasmids. of 874 e. coli strains isolated from 283 wild serows in 1980-1981, only 11 (1.3%) were resistant to at least one of 6 antimicrobial drugs; ampicillin, streptomycin, tetracycline, chloramphenicol, kanamycin and sulfadimethoxin. seven (2.5%) individuals w ...19921420561
the bronchial tree and blood vessels of the japanese serow lung.the right lung is constituted of the bilobed upper, middle, accessory and lower lobes. the left lung consists of the bilobed middle and lower lobes. the bronchial ramification and lobular divisions are similar to those of the goat lung.19863706770
histopathologic studies on mucosal and cutaneous lesions in contagious papular dermatitis of japanese serow (capricornis crispus). 19846541267
electron microscopy on mucosal and cutaneous lesions in contagious papular dermatitis of japanese serow (capricornis crispus). 19846541268
eimeria gozaishoensis n. sp. from the formosan serow (capricornis crispus swinhoei).eimeria gozaishoensis n. sp. was found in the formosan serow (capricornis crispus swinhoei). the oocysts were ovoid, 29.41 +/- 0.58 x 20.77 +/- 0.41 microns with a bilayered wall. a micropyle and micropylar cap were observed, but a polar granule and oocyst residuum were absent. sporocysts were ovoid, 11.78 +/- 0.30 x 7.60 +/- 0.31 microns, with sporocyst residuum and stieda body. the new species differs from other known species of the genus by the morphology of oocysts and that domestic goats ap ...19912067043
plasmids and lack of pathogenicity of yersinia enterocolitica isolated from wild-living birds, japanese serows and environmental specimens. 19873613350
eimeria capricornis n.sp., e. nihonis n.sp., e. naganoensis n.sp., and e. kamoshika n.sp. (protozoa: eimeriidae) from the japanese serow, capricornis crispus.eimeria capricornis n.sp., e. nihonis n.sp., e. naganoensis n.sp. and e. kamoshika n.sp. were detected from the japanese serow, capricornis crispus. the oocysts of these species were ovoid to ellipsoid, measuring 48.02 +/- 0.53 x 33.93 +/- 0.35 microns, 29.73 +/- 0.45 x 21.01 +/- 0.29 microns, 20.04 +/- 0.05 x 15.77 +/- 0.04 microns and 30.02 +/- 4.24 x 14.58 +/- 2.03 micron, respectively. in all the species the micropyle was observed, but the micropylar cap and oocyst residuum were not present. ...19892927023
serologic studies on five selected zoonoses in wild japanese serows (capricornis crispus). 19873430913
[comparative analysis of inorganic components in japanese serow horn (capricornis crispus crispus), bovine bone and human dental enamel]. 19883165425
widespread of parapox infection in wild japanese serows, capricornis crispus. 19863029495
morphology of the interdigital glands of a formosan serow (capricornis crispus swinhoei).the morphology of the interdigital glands of a male 9-year-old formosan serow was examined. the glands were sock-shaped depression of the skin and were present in four feet. the glands were composed of a neck and a body. the epidermis of inner wall of the glands was keratinized stratified squamous epithelium. apocrine glands were developed, while the sebaceous glands and hair follicles were very poorly developed. contents, probably dirt, were small in the interdigital glands. these findings rese ...19958593314
characterization of parapoxviruses circulating among wild japanese serows (capricornis crispus).we antigenically and molecularly compared 5 parapoxvirus isolates and 7 viral dna samples from clinical lesions of japanese serows with 3 viruses from sheep and goats. all isolates from japanese serows except one, ishikawa-s, reacted with six monoclonal antibodies to orf virus (orfv). restriction endonuclease analysis using amplified viral dna showed the orfv-specific pattern in all samples except ishikawa-s, which showed a bovine papular stomatitis virus (bpsv)-specific pattern. partial nucleot ...200212363024
molecular phylogeny based on the kappa-casein and cytochrome b sequences in the mammalian suborder ruminantia.nucleotide sequences for the kappa-casein precursor proteins have been determined from the genomic dnas or hair roots of the ruminantia. the coding regions, exons 2, 3, and 4, were amplified separately via the three kinds of pcrs and then directly sequenced. the primers were designed from the sequence of bovine kappa-casein gene; they were applicable for the amplification of the kappa-casein genes from the 13 species in the ruminantia except exon 2 of the lesser mouse deer. these results permitt ...19958587130
isolation and antibody prevalence of a parapoxvirus in wild japanese serows (capricornis crispus).an epizootic suspected to be caused by parapoxviruses occurred in winter, 1984-85, among wild japanese serows (capricornis crispus) with nodular or papular lesions in gifu prefecture, japan. virus isolations were attempted on 30 animals using bovine fetal testicle cell cultures. viral agents growing with cytopathic effects were isolated from six animals. the agents were identified as parapoxvirus from electron microscopic findings and physico-chemical characteristics. antibody prevalence to the ...19938394942
the lamination of the masseter muscle in the japanese serow (capricornis crispus).the lamination of the masseter muscle in 21 japanese serows of different sexes and ages was studied by the method of yoshikawa et al. who proposed a lamination theory for this muscle. the masseter muscle in the japanese serow was found to be composed of i) the proper masseter muscle which included 1) the first superficial, 2) the second superficial, 3) the intermediate and 4) the deep masseter muscles, in which the deep masseter muscle could be subdivided into a pars anterior and pars posterior ...19892748077
a comparative study on s-100 protein-immunoreactive cells in lymph nodes.s-100 protein-immunoreactive cells of the lymph node were examined in the duck and 9 mammalian species, such as guinea pigs, dogs, cats, horses, pigs, goats, cows, japanese serows and crab-eating monkeys. s-100 protein was detected in follicular dendritic cells (fdc) and tangible-body macrophages (tbm), sinus and parenchymal macrophage (mp), sinus endothelial cells (sec) and interdigitating reticulum-like cells (irc) in the node of mammalian species, but not in the duck except nervous elements. ...19902280486
onchocerca suzukii n. sp. and o. skrjabini (= o. tarsicola) from a relict bovid, capricornis crispus, in japan.the species from the tendons and sub-cutaneous tissue of the tarsal and carpal joints is identified as o. skrjabini rukhlyadev, 1964 (this taxon, which was poorly defined and badly restored, is used instead of o. tarsicola to avoid dispute). o. skrjabini belongs to on homogeneous group of four species parasitic in palearctic cervids, which seems to have evolved relatively recently. the morphological characteristics of the parasite of capricornis are not sufficient to warrant the creation of a ne ...19949140501
serological evidence of coxiella burnetii infection in wild animals in japan.one hundred and thirty-four (26%) of 511 sera from 11 wild animal species in eight prefectures in japan had antibody titers to coxiella burnetii by the enzyme-linked immunosorbent assay. high prevalences were observed in japanese black bears (ursus thibetanus) (78%), hokkaido deer (cervus nippon yesoensis) (69%), japanese hares (lepus brachyurus) (63%), japanese deer (cervus nippon centralis) (56%), and to some extent in japanese monkeys (macaca fuscata) (28%). a low prevalence (13%) was observe ...19938355353
the nerve cell bodies found in the ovine omasal laminae.a histological study was made on the omasum of sheep, cows, sika deer, and japanese serows in order to confirm the existence of the submucosal nerve cell bodies in the omasal laminae. in the present study, seventeen submucosal nerve cell bodies were found in seven sections from four out of twenty-two sheep.19948204748
maceration of fetus in the japanese serow (capricornis crispus).out of 2,138 pregnant japanese serows (capricornis crispus), one case of the maceration of fetus was found in the winter of 1991, and examined pathologically. because the fetus was covered with the hairs in the head and the neck regions, the fetus seemed to be implanted in the last breeding season and retained. the endometrium lacked the placentomes completely and showed metaplasia to the keratinized or non-keratinized squamous epithelium. in the non-keratinized region, the accumulation of neutr ...19948204747
macroscopic observations of mammary glands and teats of japanese serows, capricornis crispus, with special reference to past gestation.mammary glands and teats were examined to estimate the reproductive history of japanese serows. the presence of milk was an indicator of reproduction in the previous year. the thickness of the mammary glands is indicative of their function. the females whose mammary glands are more than 10 mm thick may be regarded as having delivered half a year before. the distance between teats is not always indicative of the function of the mammary glands. animals with teats 10 mm or less in length and those ...19957548396
pathology of interdigital glands in a wild japanese serow (capricornis crispus) infected with parapoxvirus.the interdigital glands of a japanese serow infected with parapoxvirus had severe papular and nodular lesions that completely occupied the sac and duct of the gland. the lesions were characterized by acanthosis with hyperkeratosis. intracytoplasmic inclusion bodies were detected in the vacuolated prickle cells. by electron microscopy, mature and immature viral particles were present in the cytoplasm. these glands act as scent glands, and lesions in this organ probably affected the ecological ada ...19979409527
morphology of the tongue of a male formosan serow (capricornis crispus swinhoei).the morphology of the tongue of an adult formosan serow was examined to compare it with that of the japanese serow by macroscopical and scanning electron microscopical observations. the tongue was 13.5 cm in length and 82 g in weight. the apex showed a u-shaped outline. there were 340 fungiform papillae and 23 vallate papillae on both sides. filiform, fungiform, conical and vallate papillae were found. annular pads surrounding the vallate papillae were poorly developed. no lenticular or foliate ...19989505441
occurrence of yersinia enterocolitica in wild-living birds and japanese serows.yersinia spp. were isolated from 34 of 500 birds representing nine species. the highest isolation rate, 5 of 21 (23.8%), was found in blue magpies (cyanopia cyanus), followed by pheasants (phasianus colchicus tohkaidi), 5 of 33 (15.2%); gray starlings (sturnus cineraceus), 6 of 57 (10.5%); tree sparrows (passer montanus), 1 of 14 (7.1%); bulbuls (hypsipetes amaurotis), 4 of 57 (7.0%); crows (corvus levailantii or corvus corone), 7 of 117 (6.0%); eastern turtledoves (streptopelia orientalis), 4 o ...19853977310
postnatal development and histology of the infraorbital glands in the japanese serow, capricornis crispus. 19827162003
onchocerciasis in wild japanese serows, capricornis crispus. 19827162000
pathological study on lungworm disease in the wild japanese serow, capricornis crispus. 19817289342
serological surveillance of lyme borreliosis in wild japanese serows (capricornis crispus).through serological surveillance of wild animals by enzyme linked immunosorbent assay with protein g (pg-elisa), we obtained epidemiological data on lyme borreliosis in japanese wild animals. in this study, we carried out serological surveillance for lyme borreliosis in wild japanese serows (capricornis crispus). forty-six of 200 (23%) serum samples were positive, indicating that lyme borreliosis is prevalent in wild japanese serows. this positive rate was relatively higher than that of other an ...19989673949
cercopithifilaria shohoi n. sp. (nematoda: filarioidea) from the relict bovidae, capricornis crispus, in japan.cercopithifilaria shohoi n. sp. was found in the relict bovid, capricornis crispus, in japan, and is described and compared with other species in the genus. adult male and female worms were found in subcutaneous tissues of the trunks of 6 serows shot in mt. zao, yamagata prefecture, in the northern part of honshu, the largest island of japan. the one complete male found was 19.7 mm long, and the five females were 31.6-50.9 mm long. unsheathed or sheathed microfilariae 104-122 microns long were t ...19989754307
use of protein ag in an enzyme-linked immunosorbent assay for screening for antibodies against parapoxvirus in wild animals in japan.using protein ag in an enzyme-linked immunosorbent assay (elisa), we tried to detect antibodies against parapoxvirus in 9 species of wild animals in japan: the japanese badger (meles meles anakuma), japanese black bear (ursus thibetanus japonicus), japanese deer (cervus nippon centralis), japanese monkey (macaca fuscata), japanese raccoon dog (nyctereutes procyonoides viverrinus), japanese serow (capricornis crispus), japanese wild boar (sus scrofa leucomystax), masked palm civet (paguma larvata ...199910225841
premolar and molar rotation in wild japanese serow populations on honshu island, japan.the skulls of 1195 japanese serow (capricornis crispus) from six geographically distinct populations were examined for tooth rotation. our objectives were to determine tooth rotation patterns in japanese serows, investigate geographical variation in tooth rotation and examine whether space limitations in the jaw accounted for variations. we then sought to explain the origin of tooth rotation in the japanese serow. rotated teeth were found in 131 specimens (62 males, 57 females and 12 unknown gen ...200616901458
morphology of the dorsal lingual papillae in the barbary sheep, ammotragus lervia.the dorsal lingual surface of a barbary sheep (ammotragus lervia) was examined by scanning electron microscopy (sem). the tongue was about 20 cm in length. there were about 30 vallate papillae on both sides. filiform, conical, fungiform and vallate papillae were found. the filiform papillae were distributed over the entire dorsal surface of the tongue, excepted for the lingual torus where conical papillae were present. the fungiform papillae were present rounded bodies, and more densely distribu ...200010998934
olecranon lesions caused by onchocerca skrjabini in wild japanese serows (capricornis crispus).wild japanese serows (capricornis crispus) were found to have parasitic lesions in tendons that attached the musculus tricepus brachii to the olecranon. histopathological study of the lesions showed chronic tendinitis with multiple granulation nodules around the worms. the lesions were found in 138 of the 353 serows examined and were more frequent in aged animals than young ones. transverse ridges on the cuticle of the female midbody, the sizes and morphological features of the spicules, and the ...19979192361
tissue distribution of heavy metals and their variations with age, sex, and habitat in japanese serows (capricornis crispus). 19873632041
diversification of cercopithifilaria species (nematoda: filarioidea) in japanese wild ruminants with description of two new species.twelve of the 17 cervus nippon nippon deer from kyushu island, japan, that we examined were infected with one or two cercopithifilaria species. c. longa n. sp. adults were in the subcutaneous tissues of limbs and the abdomen, and c. crassa n. sp. adults were in the skin, mainly in the anterior part of the back; the distribution of the dermal microfilariae generally matched that of the adult worms. the two new species were assigned to the group of primitive cercopithifilaria species that parasiti ...200212514943
immunohistochemical evaluation of the muscularis mucosae in the ruminant forestomach.the muscularis mucosae and condensed fibrous layer of the ruminant forestomach were studied by immunohistochemistry using specific antibodies against alpha-smooth muscle actin (alphasma) and gamma-smooth muscle actin (gammasma). the specimens were collected from the rumen, reticulum and omasum of cattle, water buffalo, sheep, goat, barbary sheep, japanese serow, sika deer and mouse deer. the muscularis mucosae showed immunoreactivity for both alphasma and gammasma. on the other hand, the condens ...200312823104
eimeria species from serows (capricornis spp.) in japan with descriptions of two new species.fresh fecal samples from 35 japanese serows capricornis crispus (temminck, 1845), and 5 formosan serows capricornis swinhoei gray, 1862, were collected between october 1998 and march 1999. eimeriid coccidians were found in 12 (34%) japanese serows and in 1 (20%) formosan serow. three eimeria species were found, and 2 are described here as new. the third species is consistent with the description of eimeria kamoshika inoue, 1989. sporulated oocysts of 1 new species are broadly ellipsoidal, 20.7 x ...200312880260
morphological study of the forestomach of the japanese serow (capricornis crispus).details of the morphology of the forestomach of the japanese serow (capricornis crispus) were examined macroscopically, and by light and scanning electron microscopy. the rumen was sac-like in shape, and the right accessory longitudinal groove was not visible externally. the reticulum was relatively small, while the omasum was oval and large. inside the rumen, even in the dorsal sac, the ruminal papillae were densely distributed, although absent on the pillars. the caudodorsal coronary pillar wa ...19989591368
coexistence of five cercopithifilaria species in the japanese rupicaprine bovid, capricornis crispus.the japanese serow, capricornis crispus (bovidae, caprinae, rupicaprini), is parasitized by five cercopithifilaria species: c. shohoi, recently described, and reexamined in this paper, c. multicauda n. sp., c. minuta n. sp., c. tumidicervicata n. sp., and c. bulboidea n. sp. coinfections are frequent. the location (skin or subcutaneous regions) in the host of adult worms differed between the species, as did many morphological characters of both adults and microfilariae. the location (limbs, trun ...200111584750
detection and diagnosis of parapoxvirus by the polymerase chain reaction.the genus parapoxvirus includes four members, bovine papular stomatitis virus (bpsv), pseudocowpox virus (pcpv), orf virus (orfv) and parapoxvirus of red deer in new zealand (pvnz). a set of primers for polymerase chain reaction (pcr) was designed to detect viral dna from cells infected with each of the four parapoxviruses. the set of primers resulted in the amplification of appropriately sized products from cells infected with bpsv, pcpv, orfv and pvnz, respectively. the pcr method was applied ...200010680970
mycoplasma ovis detected in free-living japanese serows, capricornis crispus.nineteen blood samples collected from free-ranging wild japanese serows, capricornis crispus, between 2006 and 2008 in iwate prefecture were examined for the hemoplasma infection by real-time pcr targeting the 16s rrna gene. five (26.3%) out of the 19 samples were positive in real-time pcr with an average melting temperature at 85.18 °c. the positive samples in the real-time pcr were reconfirmed by conventional pcr, and one of them was successful for direct dna sequencing. the nucleotide sequenc ...201020962460
histochemistry of glycoconjugates in the nasolabial skin of the japanese serow (capricornis crispus, artiodactyla, mammalia) with special reference to glandular structures.the histochemistry of glycoconjugates in the nasolabial skin of the japanese serow ( capricornis crispus ) was studied by light microscopic histochemical methods, particularly lectin histochemistry. the eccrine glands present exhibited neutral and acidic glycoconjugates with different saccharide residues (alpha-l-fucose, beta-d-galactose, beta-n-acetyl-d-glucosamine, alpha-d-galactose and n-acetyl-neuraminic acid) especially in the cells of the secretory acini, the free surface of the collecting ...200315121549
chorioptic mange in a wild japanese serow.parasitologic and histopathologic examinations were performed on a wild japanese serow (capricornis crispus) with severe dermatitis from tochigi, japan in february 1999. the serow was infested with a large number chorioptes bovis. marked parakeratotic hyperkeratosis was characteristic of the lesions induced by these mites. japanese serow is a new host of c. bovis.200312910774
phylogeny of sheep and goat theileria and babesia parasites.the phylogenetic relationship of theileria and babesia species infecting sheep and goats on the basis of their 18s rna gene structure was addressed in the present study. for this purpose, the complete sequences of the small ribosomal rna genes of a panel of sheep and goat piroplasm isolates, including t. lestoquardi, t. ovis, t. separata, b. ovis, b. motasi, b. crassa and several novel species, were sequenced and compared. the classification based on the established phylogenetic tree corresponde ...200314505044
genotyping of clostridium perfringens isolated from domestic and exotic ruminants and swine.clostridium perfringens types a, b, c, d and e are known to cause severe enteritis/enterotoxaemia and diseases (especially caused by type a) belonging to the gas oedema complex in many species. samples from the small intestine as well as faeces of domestic and exotic animals suffering from enterotoxaemic signs or having died within days after first occurance of toxaemia were submitted for typing c. perfringens toxovars by multiplex pcr. the following species have been investigated: domestic shee ...200314535937
absence of the functional myosin heavy chain 2b isoform in equine skeletal muscles.nucleotide sequences which included the full coding region for three types of myosin heavy chain (myhc) isoforms were determined from equine skeletal muscles. the deduced amino acid sequences were 1937, 1938, and 1935 residues for the myhc-2a, -2x, and -slow, respectively. no myhc-2b isoform was amplified from the equine muscle cdna except for one pseudogene fragment. one nucleotide was inserted in the coding region of the equine pseudogene product, a minute amount of which was expressed in the ...200415170063
isolation and partial characterization of a parapoxvirus isolated from a skin lesion of a weddell seal.a solitary skin lesion was found on the neck of a weddell seal (leptonychotes weddellii), chemically immobilized in queen maud land (70 degrees 09's, 05 degrees 22'e) antarctica 2001. the lesion was elevated and 3cm in diameter, consisting of partly fresh and partly necrotic tissue, and proliferative papilloma-like structures were seen. electron microscopy on a biopsy from the lesion revealed typical parapoxvirus particles. polymerase chain reaction (pcr; b2l gene) generated amplicons of approxi ...200515681058
molecular phylogenetic status of the korean goral and japanese serow based on partial sequences of the mitochondrial cytochrome b gene.to investigate the molecular phylogenetic status of the korean goral, nemorhaedus caudatus raddeanus, and japanese serow, capricornis crispus, we determined partial sequences of the mitochondrial cytochrome b gene of twelve korean gorals and sixteen japanese serows, and compared them with those of the major lineages of rupicaprini species including two other nemorhaedus species and two other capricornis species. the korean gorals examined possessed two haplotypes with only one nucleotide differe ...200415179056
variations in the number of teeth in wild japanese serow (naemorhedus crispus).skulls of 755 japanese serow (naemorhedus crispus) were examined for numerical anomalies of teeth, and these were found in 30 (4.0%) specimens. in addition to supernumerary and congenital missing teeth, embedded teeth were also identified. supernumerary teeth were found in 10 (1.3%) specimens, congenital missing teeth were found in nine (1.2%) specimens, and embedded teeth were found in 11 (1.5%) specimens. supernumerary teeth were found among the incisors, the upper and lower premolars, and the ...200516137494
new filarial nematode from japanese serows (naemorhedus crispus: bovidae) close to parasites from elephants.a new onchocercid species, loxodontofilaria caprini n. sp. (filarioidea: nematoda), found in subcutaneous tissues of 37 (33%) of 112 serows (noemorhedus crispus) examined in japan, is described. the female worm had the characteristics of loxodontofilaria, e.g., the large body size, well-developed esophagus with a shallow buccal cavity, and the long tail with three caudal lappets. the male worm of the new species, which was first described in the genus, had unequal length of spicules, 10 pairs of ...200617007210
zoonotic onchocerciasis caused by a parasite from wild boar in oita, japan. a comprehensive analysis of morphological characteristics of the worms for its diagnosis.histological examination of a nodule removed from the back of the hand of a 58-year-old woman from oita, kyushu, japan showed an onchocerca female sectioned through the posterior region of the worm (ovaries identifiable) and young (thin cuticle). six onchocerca species are enzootic in that area: o. gutturosa and o. lienalis in cattle, o. suzukii in serows (capricornis crispus), o. skrjabini and an onchocerca sp. in cervus nippon nippon, and o. dewittei japonica in wild boar (sus scrofa leucomyst ...200415490753
molecular phylogenetic relationships among seven japanese species of cercopithifilaria.dna sequences from a portion of the mitochondrial coi gene were used to clarify phylogenetic relationships among japanese species in the genus cercopithifilaria. sequences were determined from seven japanese species, five (c. shohoi, c. multicauda, c. minuta, c. tumidicervicata and c. bulboidea) from the serow (capricornis crispus f. bovidae) and two (c. longa and c. crassa) from the sika deer (cervus nippon nippon f. cervidae). no base substitutions were observed between c. bulboidea and c. lon ...200515936981
phospholipid and fatty acid composition of erythrocyte membrane from wild japanese serow (capricornis crispus).the phospholipid composition and fatty acid composition of the individual phospholipids were determined in erythrocyte membrane of wild japanese serow, capricornis crispus, and compared with those of japanese cattle. sphingomyelin (sm) contributed more than 50% to the total phospholipids, with only 3% phosphatidylcholine, 30% phosphatidylethanolamine and 11% phosphatidylserine. this phospholipid composition and ratio of phospholipid to protein in erythrocyte membrane of wild serow were quite sim ...19846697694
serological survey of parapoxvirus infection in wild ruminants in japan in 1996-9.the prevalence of parapoxvirus infection was examined in free-ranging wild ruminants in japan, japanese serow (capricornis crispus) and japanese deer (cervus nippon centralis), in 1996-9. we collected a total of 151 serum samples from 101 japanese serows and 50 japanese deer and tested for antibodies against parapoxvirus by an enzyme-linked immunosorbent assay and an agar gel immunodiffusion test. overall seroprevalences among japanese serows were 5/25 (20.0%) in 1996, 4/14 (28.6%) in 1997, 5/32 ...200111293676
model of japanese serow (capricornis crispus) energetics predicts distribution on honshu, japan.understanding what determines a species' range is a central objective in ecology and evolutionary biology. it has important applications for predicting species distributions and how they might respond to environmental perturbations. this paper describes a mechanistic approach to predict past and present distribution of the japanese serow (capricornis crispus) on honshu, japan. we applied state-of-the-art microclimate and animal biophysical/behavioral models coupled with climate and vegetation da ...200717708220
evidence for pestivirus infection in free-living japanese serows, capricornis crispus.sixteen serum samples collected from free-living japanese serows, capricornis crispus, between 2001 and 2004 in morioka and its vicinity were examined for the presence of pestivirus by reverse transcription-nested pcr procedure. three out of the 16 samples produced a visible band in electrophoresed agarose gels. the nucleotide sequences of the three pcr products were found to be identical. the pestivirus found in the serow was identified as bovine viral diarrhea virus 1 (bvdv-1) based on nucleot ...200617053318
phylogenetic analysis of the 16s rrna gene of anaplasma species detected from japanese serows (capricornis crispus).nineteen blood samples collected from free-living japanese serows, capricornis crispus, between 2006 and 2008 in iwate prefecture were examined for the anaplasma infection by pcr amplification of a part of the 16s rrna gene. ten (52.6%) out of the 19 samples produced a visible band in electrophoresed agarose gels. positive pcr products were subjected to dna sequencing. we found the nucleotide sequences were identical. almost entire length of the 16s rrna gene for a representative stain was then ...200920046041
molecular and biological characterization of vascular endothelial growth factor of parapoxviruses isolated from wild japanese serows (capricornis crispus).contagious dermatitis in domestic and wild ruminants caused by parapoxvirus (ppv) occurs worldwide. although ppv infections appear in cattle, sheep and goats, the papular, nodular, pustular and ulcerated skin lesions of wild japanese serows (capricornis crispus) are significantly more severe than those of other animals. to determine the factors involved in the severity of these skin lesions, we compared the molecular characteristics of 4 ppv isolates from japanese serows and 2 isolates from shee ...201019716664
tooth size variability and relevance of numerical variation in the japanese serow.we analysed the variability of the lower cheek teeth of the japanese serow, capricornis crispus, from aichi prefecture, japan, based on the coefficient of variation (cv), and examined tooth size variability. the mesiodistal (md) and buccolingual (bl) crown diameters of permanent cheek teeth were measured, and an index of the crown surface (mdxbl) was calculated. as a result, the cv for md measurements ranged from 3.6 to 6.3, and that for bl measurements ranged from 3.6 to 6.5. in males and femal ...200817920033
genetic heterogeneity among parapoxviruses isolated from sheep, cattle and japanese serows (capricornis crispus).standard strains of four parapoxviruses and seven unclassified japanese strains isolated from sheep, cattle and wild japanese serows (capricornis crispus) were compared molecularly. restriction fragment length polymorphism (rflp) analysis of viral dna, indirect immunofluorescence assays using monoclonal antibodies, partial nucleotide sequencing of the envelope gene, phylogenetic analysis and pcr-rflp were carried out. these analyses revealed that the parapoxviruses were divided into four groups ...200111297696
direct evidence for toxoplasma gondii infection in a wild japanese serow (capricornis crispus) in mainland japan.abstract toxoplasma gondii infection was studied in 41 japanese serows (capricornis crispus), a goat-antelope in mainland japan. blood and muscle specimens were collected from 41 subjects between 2006 and 2010. presence of antibodies to t. gondii in the sera was examined by using the latex agglutination test (cut-off titer 1:32); 10 of 41 (24.4%) were seropositive. t. gondii dna was detected in muscle tissue of 1 seropositive serow by using a semi-nested pcr assay for the b1 gene of t. gondii. ...201121882974
infective larvae of cercopithifilaria spp. (nematoda: onchocercidae) from hard ticks (ixodidae) recovered from the japanese serow (bovidae).hard ticks taken from the japanese serow, capricornis crispus, in yamagata prefecture, honshu, harboured infective larvae of onchocercid filariae after incubation from the 22nd to the 158th day. haemaphysalis flava and h. japonica contained one to eight filarial larvae; females, males and a nymph of the ticks were infected. the 44 infective larvae recovered were 612-1,370 μm long, and 11 of them, 930-1,340 μm long, were studied in detail. the larvae possessed the morphologic characteristics of t ...201323340227
a core set of microsatellite markers for conservation genetics studies of korean goral (naemorhedus caudatus) and its cross-species amplification in caprinae species.in order to screen microsatellites for conservation genetics studies of the species, a total of 23 microsatellite loci from korean goral (naemorhedus caudatus), including 15 previously developed loci and 8 new loci in this study, were tested. eleven microsatellites were screened and subjected to cross-species amplification using a test panel of four caprinae species, japanese serows (capricornis crispus), chinese gorals (naemorhedus goral), northern chamois (rupicapra rupicapra) and domestic goa ...201021113106
molecular phylogenetic analysis of theileria species detected from japanese serows (capricornis crispus) in iwate prefecture, japan.in the present study, we tried to detect dna for ribosomal rna genes of piroplasma parasites from the liver or blood of 43 japanese serows (capricornis crispus) in iwate prefecture of japan by polymerase chain reaction. approximately 500-bp amplicons were obtained in 35 (81.4%) of the 43 samples by amplification for v4 hyper-variable regions of the 18s rrna gene, and the amplicons were considered to be dna of theileria species. the complete nucleotide sequences (1,700 or 1,709 bp) of the 18s rrn ...201121646752
involvement of two genetic lineages of sarcoptes scabiei mites in a local mange epizootic of wild mammals in japan.similar to wild mammals on the continents, mange caused by the mange mite, sarcoptes scabiei (acari: sarcoptidae) is spreading in wild mammals in most of japan. we collected crusted or alopetic skin from 120 raccoon dogs (nyctereutes procyonoides viverrinus), three raccoons (procyon lotor), six japanese badgers (meles anakuma), one japanese marten (martes melampus), one stray dog (canis lupus familiaris), four wild boars (sus scrofa leucomystax), and one japanese serow (capricornis crispus), mai ...201525397999
spatial and temporal genetic homogeneity of orf viruses infecting japanese serows (capricornis crispus).this study genetically characterized orf viruses (orfvs) isolated from recent outbreaks in japanese serows (capricornis crispus) in 2007 and 2008, and from earlier outbreaks from 1985 to 2001. nucleotide sequences of genes for the viral envelope, vascular endothelial growth factor (vegf), and virus interferon resistance (vir) were determined in two orfvs isolated from recent outbreaks and in eight from earlier outbreaks. no deletions or insertions were observed in these genes. surprisingly, the ...201020124763
morphology of the dorsal lingual papillae in the blackbuck, antilope cervicapra.the dorsal lingual surface of a blackbuck (antilope cervicapra) was examined by scanning electron microscopy (sem). the tongue was about 125 mm in length. there were about 30 vallate papillae on both sides. filiform, conical, fungiform and vallate papillae were found. the filiform papillae were distributed over the entire dorsal surface of the tongue, excepted for the lingual torus where conical papillae were present. the fugiform papillae were present rounded bodies, and more densely distribute ...199910693328
dicroceliasis in the wild japanese serow, capricornis crispus. 19846482135
reproduction and prenatal growth in the wild japanese serow, capricornis crispus. 19817339141
ciliate protozoa from the rumen of the japanese serow, capricornis crispus (temminck). 19816798292
pathological study on cutaneous papillomatosis in a japanese serow (capricornis crispus). 1976988413
influences of different large mammalian fauna on dung beetle diversity in beech forests.this paper focuses on biological relationships between mammalian species richness and the community structure of dung beetles in cool-temperate forests in the northernmost part of mainland japan. the composition of beetle assemblages was evaluated at 3 sites in undisturbed beech forests with different mammalian fauna. in spring and summer 2009, beetles were collected at each site using pitfall traps baited with feces from japanese macaques, macaca fuscata blyth (primates: cercopithecidae); asiat ...023909510
establishment of vascular endothelial cell lines from the aortas of wild japanese serows (capricornis crispus).endothelial cell lines were established from the aortas of wild japanese serows (capricornis crispus) by transfection of a simian virus 40 (sv40) large t antigen gene. the cloned cell lines, designed seet (japanese serow endothelial-sv40t) cells, express sv40t antigen and retain cobblestone-like morphology. although von willbrand factor (vwf) is expressed in the cells, the expression rate and the quantity are lower than in serow primary endothelial cells. the seet cells exhibit positive uptake o ...200919269341
the lumbosacral plexus of the japanese serows, capricornis crispus.the lumbosacral plexus of the japanese serow was grossly investigated on 30 fresh specimens. 28 of 30 showed 6 lumbar vertebrae and 2 of the remaining had 5. the communicating pattern among segmental origins from l3 to s5 revealed a variability of the plexus in both types of 5 and 6 lumbar vertebrae. the ratio of prefixed, median fixed and postfixed plexuses in 6 lumbar vertebral type was nearly 2:6:1. the serow's plexus was basically similar to that of domestic ruminants.19873425938
genetic variation and phylogenetic relationship between three serow species of the genus capricornis based on the complete mitochondrial dna control region sequences.the molecular evidence of phylogenetic status regarding the formosan serow (capricornis swinhoei) is not robust and little is known about the genetic diversity of the sumatran serow (capricornis sumatraensis), which partly is due to the hardness in sample collection. here we determined the sequences of the complete mitochondrial dna control region (1,014 bp) of 19 sumatran-serow individuals. nine new haplotypes were defined based on 78 variable sites. combined analysis with other 32 haplotypes d ...201324057256
molecular genetic conspecificity of spiculopteragia houdemeri (schwartz, 1926) and s. andreevae (dróżdż, 1965) (nematoda: ostertagiinae) from wild ruminants in japan.male dimorphism of the subfamily ostertagiinae (nematoda: trichostrongylidae) is a well-known phenomenon, and two or more morphotypes of a single species have previously been described as different species. two spiculopteragia spp., s. houdemeri (syn. s. yamashitai) and s. andreevae (syn. rinadia andreevae) recorded in asian cervids and wild bovids, are considered to represent major and minor morphs of s. houdemeri, respectively, based solely on their co-occurrence in the same host individual al ...201423168162
infraorbital glands of a male formosan serow (capricornis crispus swinhoei).the morphology of the infraorbital glands of a male formosan serow was examined. the glands consisted of an inner sebaceous portion and an outer apocrine portion. the inner sebaceous portion was much larger than that of the male japanese serow. the sebaceous portion of the gland consisted of the ordinary and modified types of tissue that are present in the infraorbital gland of the female japanese serow, and the modified tissue contained some melanin granules and melanocytes. the apocrine portio ...19969090995
the mineralization of crystalline inorganic components in japanese serow horn.the japanese serow (capricornis crispus) is protected as a special natural monument in japan. the ring count of the soft x-ray photographs of japanese serow horn was found to be a useful criteria to determine the ages exactly. the mineralization process in japanese serow horn was examined microscopic, icp and x-ray diffraction methods. the incremental lines appeared as light and dark layers in the section stained for fuchsin and methylen blue. mineral depositions were observed among the keratin ...19958868213
an historical overview of selected rare ruminants in captivity.in assessing the situation revealed by the survey, we concluded that in general when sufficient numbers of founders were available and attention paid to the animals general and specific needs, including preventative medicine, the captive populations have done reasonably well. is is obvious that some small groups, such as the small african antelope and the duikers, need highly specialised care and there is a great deal of work still to be done on their husbandry. the mountain or alpine species ha ...198632336816
myoepithelial cells and innervation in the infraorbital gland of the japanese serow (capricornis crispus).both the apocrine and the sebaceous portions of the infraorbital gland of the japanese serow were examined to obtain morphological evidence related to the mechanism of secretion. the examination was carried out by an immunohistochemical method using antibodies against alpha-smooth muscle actin for myoepithelial cells and protein gene product 9.5 for nerve fibers. immunostaining for alpha-smooth muscle actin was apparent in the myoepithelial cells of apocrine tubules and varied within the same gl ...19958534577
zygomatic salivary glands in japanese serows, capricornis crispus. 19846492545
apocrine secretion in the infraorbital gland of the japanese serow, capricornis crispus: a scanning electron-microscopic study.the infraorbital gland of the japanese serow was examined under the scanning electron microscope to clarify the nature of apocrine secretion. the size and number of apocrine blebs on secretory cells varied markedly from cell to cell; as the size of blebs increased, their numbers decreased. after pinching off of blebs, crown-shaped structures were retained on the remaining apical surface of secretory cells. detached free blebs in the lumen were spherical in shape with smooth surfaces. secretory c ...19938273450
comparative morphological study on the lingual papillae and their connective tissue cores in rabbits.the morphological structure of the lingual papillae and their connective tissue cores (ctc) in a rabbit were studied using lm and sem and were compared to that of other animal species. externally, the filiform papillae distributed on the anterior surface of the dorsal tongue were short and conical with a round base and had a flat area on their anterior upper half. the ctc of the conical filiform papillae had a roughly triangular plate-like structure with a round top. several small round protrusi ...200818975613
ulex europaeus agglutinin i binding in the apocrine glands of the interdigital gland and skin in the japanese serow capricornis crispus. 19892927027
lectin histochemistry of the interdigital gland in the japanese serow (capricornis crispus) in winter.the interdigital gland of the japanese serow was examined by histological and lectin histochemical techniques. the gland is composed of a thin-walled pouch and a duct. both regions contain sebaceous and apocrine glands, but the development of each component was significantly less marked than those of the skin in the region. in particular, only a small amount of sebaceous and apocrine glandular elements was found in the pouch, although they were more abundant in the duct. histochemical staining o ...19883254889
plasma and fecal sex steroid hormone profiles during the estrous cycle in a japanese serow (capricornis crispus).we aimed to determine plasma progesterone and estradiol-17beta concentrations, as well as fecal progesterone concentrations during the estrous cycle in a female 8-year-old japanese serow (capricornis crispus). the step frequencies during the night were recorded by a pedometer attached to the serow's hind leg from october to the following june. estrous behavior was also monitored during the day. blood samples were taken once a day from 8 february to 10 march from an indwelling catheter placed in ...200919404003
the preputial gland of the japanese serow capricornis crispus: ultrastructure and lectin histochemistry.preputial glands of the japanese serow were found to consist of the modified sebaceous gland and apocrine gland in both sexes. in the modified sebaceous gland, cells transformed gradually toward the lumen from plentiful cytoplasm to a sponge-like network. lipid droplets were observed in mitochondria of differentiating cells. by lectin histochemistry, differentiating cells were stained with arachis hypogaea (pna), triticum vulgaris (wga) and canavalia ensiformis (con a) lectins, while secretory c ...20092728845
genetic characterization of protostrongylus shiozawai from japanease serows (capricornis crispus).abstract protostrongylus shiozawai (nematoda: strongylida) infection is known to occur in the lungs of wild japanese serows (capricornis crispus), and to date, has been classified only by its morphological characteristics, as well as host specificity. to characterize p. shiozawai genetically, a partial sequence of the second internal transcribed spacer (its-2) region was determined and compared with those of related nematodes. our subsequent classification of p. shiozawai based on phylogenetic a ...201121787212
distinct distribution of dicrocoelium dendriticum and d. chinensis in iwate prefecture, japan, and a new final host record for d. chinensis.this study dealt with the morphological and molecular identification of dicrocoelium flukes obtained from japanese serow (capricornis crispus) and sika deer (cervus nippon centralis) in the twelve districts of iwate prefecture, japan. dicrocoelium dendriticum and d. chinensis were exclusively detected in the western, and coastal and eastern areas of iwate prefecture, respectively. this geographically distinct occurrence of the two dicrocoelium species would be associated with the distribution of ...201424998394
the brachial plexus of the japanese serow (capricornis crispus).the brachial plexus of the japanese serow was macroscopically studied. it was composed of nerves derived from the sixth (c6), seventh (c7) and eighth (c8) cervical nerves and the first (t1) thoracic nerve (98.1%). rarely, the second t2) thoracic nerve joined it (1.9%). c6 joined c7 and made up the cranial trunk. c8 and t1 (+t2) united to form the caudal trunk. each trunk split into dorsal and ventral divisions. the dorsal divisions from c6-t1 formed the dorsal cord which gave off the thoracodors ...19873565786
blackfly vectors of zoonotic onchocerciasis in japan.studies of blackfly vectors of onchocerca dewittei japonica uni, bain & takaoka (spirurida: onchocercidae), a parasite of wild boar implicated in the aetiology of zoonotic onchocerciasis in japan, and six other zoonotic onchocerca species of this country are reviewed. molecular identification of infective larvae found in wild-caught female blackflies showed that simulium bidentatum (shiraki) (diptera: simuliidae) is a natural vector of o. dewittei japonica, and also onchocerca sp. sensu fukuda e ...201222827756
characterization of dicrocoelium dendriticum isolates from small ruminants in shaanxi province, north-western china, using internal transcribed spacers of nuclear ribosomal dna.the genetic variations in internal transcribed spacers (its) spanning its-1, 5.8s and its-2 rdna of dicrocoelium dendriticum, isolated from sheep and goats in four geographical regions in shaanxi province, were examined. the lengths of its-1, 5.8s and its-2 rdna sequences for d. dendriticum were 749 bp, 161 bp and 234 bp, respectively. intra-specific sequence variations of d. dendriticum were 0-0.5% for its-1 and 0-1.3% for its-2 rdna, while the inter-specific variations among species in genus d ...201523890204
establishment of an on-site diagnostic procedure for detection of orf virus from oral lesions of japanese serows (capricornis crispus) by loop-mediated isothermal amplification.orf virus infection has been prevalent continuously in the population of wild japanese serows (capricornis crispus), goat-like grazing cloven-hoofed mammal species that live mainly in mountainous areas of japan. currently, definitive diagnosis of infection requires time-consuming laboratory work. to diagnose rapidly on-site, we developed a field-friendly procedure for the detection of orf virus from oral cavity lesions. dna was extracted from goat saliva spiked with orf virus as a proxy for japa ...201727628591
lectin histochemical study on the infraorbital gland of the japanese serow (capricornis crispus).histology and lectin histochemistry were performed in the infraorbital gland of the japanese serow. the gland is composed of glandular tissues and a pouch filled with the secretion. the tissues consist of an inner layer of sebaceous glands and an outer layer of apocrine glands. the male sebaceous layer is made up of the ordinary type, whereas the female's layer consists of the ordinary and modified types. in the apocrine gland stained with arachis hypogaea (pna), nine different patterns of gland ...19873506377
a novel and rapid diagnostic method for discriminating between feces of sika deer and japanese serow by loop-mediated isothermal amplification.severe damages to natural vegetation, agriculture, and forestry caused by overpopulation of sika deer (cervus nippon) have markedly increased in japan in recent years. to devise a population management plan of sika deer, information on the distribution and population size of the animal in each region is indispensable. an easy and effective method to obtain this information is to count the fecal pellets in the field. however, the habitat of sika deer in japan overlaps that of japanese serow (capr ...201526084704
on-site visual diagnosis of parapoxvirus infection using a portable cordless incubator.a portable cordless incubator was developed for on-site visual diagnosis of parapoxvirus infection on farms and in areas with no electricity or laboratory equipment. the battery-powered thermoregulator can maintain a stable temperature for more than 1 h. the incubator successfully amplified parapoxvirus dna isolated from sheep and wild japanese serows (capricornis crispus) by loop-mediated isothermal amplification (lamp). although different absorbance values were obtained for the lamp reactions ...201425492467
genetic variability of 3'-proximal region of genomes of orf viruses isolated from sheep and wild japanese serows (capricornis crispus) in japan.orf virus is a prototype species of the genus parapoxvirus, subfamily chordopoxvirinae, family poxviridae. japanese orf viruses, infecting sheep and wild japanese serows (capricornis crispus), have been considered to be genetically closely related based on the sequence identities of the open reading frames (orfs) 11, 20, and 132 in their genomes. however, since the genome size of orf viruses is about 140 kbp long, genetic variation among japanese orf viruses remains unclear. in this study, we an ...202032391386
effects of the physical and social environment on flight response and habitat use in a solitary ungulate, the japanese serow (capricornis crispus).observations of the flight response of ungulates are commonly used to test behavioral responses to predation risk. in gregarious ungulates with sexual body-size dimorphism such responses are likely to be stronger in situations where individuals have perceptions of less security as well as among more-sensitive individuals, such as female groups or female groups with offspring which are understood to use safety habitats more often than males do. however, little is known about these behaviors in so ...201930585168
local variation in diet composition of the japanese serow during winter.we investigated local geographical variation in the plant composition and quality (crude protein) of the diet of the japanese serow (capricornis crispus) by analyzing rumen contents. we collected the rumen contents from 111 japanese serows culled for pest control between january and march 1997 in shizuoka prefecture, central japan. the composition of the diet, characterized by a high proportion of browse (65.2%), including green plant leaves (55.3%), confirmed that the japanese serow is a typica ...200819267649
lights and shadows in the evolutionary patterns of insular bovids.endemic bovids are intriguing elements of insular faunas. the living species include the japanese serow (capricornis crispus) and the formosan serow (c. swinhoei), the tamaraw from mindoro, philippines, (bubalus mindorensis) and the anoas (b. depressicornis and b. quarlesi), 2 species of dwarf buffalos endemic to sulawesi, indonesia. fossil endemic bovids are only recorded in some asian, north american and western mediterranean islands. here we present a comprehensive overview of the changes in ...201424673764
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