| inhibition of aldehyde reductase by acidic metabolites of the biogenic amines. | | 1975 | 16 |
| respiratory effects of h+ and dinitrophenol injections into the brain stem subarachnoid space of fetal lambs. | mock cerebrospinal fluid (ph 5.37-8.38) or 2,4-dinitrophenol (dnp) (0.15-1.5 mg) was injected into the subarachnoid space of the ventral brain stem of exteriorized fetal sheep. changes in ph on the ventral surface of the medulla did not stimulate respiratory efforts or induce significant cardiovascular changes. the respiratory response to dnp injections ranged from no response to prolonged rhythmic ventilation that was independent of the peripheral chemoreceptors or the control arterial ph and b ... | 1975 | 134 |
| phenomenon of hot-cold hemolysis: chelator-induced lysis of sphingomyelinase-treated erythrocytes. | staphylococcus aureus produces a phospholipase c specific for sphingomyelin (beta-hemolysin). erythrocytes with approximately 50% sphingomyelin in their membranes, e.g., from sheep, have been shown to have up to 60% of this phospholipid hydrolyzed by this enzyme at 37 c in isotonic buffered saline without hemolysis. cooling of sphingomyelinase c-treated erythrocytes to 4 c causes complete lysis of the cells, a phenomenon known as hot-cold hemolysis. the addition of ethylenediaminetetraacetate (e ... | 1975 | 333 |
| methods to improve detection of pneumococci in respiratory secretions. | simple methods to enhance the detection of pneumococci in respiratory secretions are needed. sheep blood agar containing 5 mug of gentamicin per ml was more often positive (89%) than either standard sheep blood agar (54%) or mouse inoculation (65%) in recovering pneumococci from 62 adult and pediatric patients. in adults, the direct quellung test on sputum smear was a rapid, sensitive method for predicting subsequent pneumococcal isolation by culture (19 of 20 patients, 95%). the quellung test a ... | 1975 | 421 |
| significance of serum pepsinogen and abomasal ph levels in a field infection of o circumcincta in lambs. | serum pepsinogen estimations from serially bled lambs grazing on pasture from spring to autumn showed correlations with the availability of ostertagia larvae on pasture, with faecal egg counts of o circumcincta, and with ostertagia worm counts in similar lambs slaughtered fort-nightly from the same pasture. in the slaughtered lambs correlations were recorded between worm count, serum pepsinogen level and abomasal ph. the value of serum pepsinogen estimations as a diagnostic test is discussed wi ... | 1975 | 842 |
| sympathico-adrenal system activity in a primary immune response. | experiments were carried out on linear mice immunized with sheep erythrocytes; it was found that the primary immune respose developed against the background of significant changes in the state of the sympathico-adrenal system, whose activity was determined by the dynamics of catecholamines in the blood and in the tissues of a number of organs, including the thymus, the spleen and the lymph nodes. by comparing the value of specific and neurohumoral indices it was revealed that the neurohumoral sh ... | 1975 | 877 |
| use of the gvh reaction to investigate the regulation of the humoral immune response. | | 1975 | 898 |
| changes in the acid base status of sheep anaesthetised with a combination of atropine sulphate acepromazine and ketamine hydrochloride. | ph, paco2, pao2, standard bicarbonate, base excess and reduced ph were measured in sheep before and at regular intervals after administration of ketamine with and without atropine and acepromazine premedication. a decrease in ph and pao2 and a rise in paco2 was observed 15 minutes after administration of ketamine. administration of atropine with and without acepromazine had no significant effect on ph, paco2 and pao2. the values for standard bicarbonate, base excess and reduced ph were not signi ... | 1975 | 986 |
| micellar solubilization of fatty acids in aqueous media containing bile salts and phospholipids. | 1. the solubility of fatty acids in aqueous media containing bile salts alone and in admixture with either lecithin (phosphatidylcholine) or phosphatidylethanolamine was determined. 2. over the ph range 2-0-7-4, the order of fatty acid solubility in aqueous solutions containing bile salts was linoleic greater than oleic greater than elaidic greater than palmitic greater than stearic. the solubility of each fatty acid increased as the ph of the miceus solutions of bile salts greatly increased the ... | 1976 | 1082 |
| antitumor and immunosuppressive activities of lankacidin-group antibiotics: structure-activity relationships. | the antitumor and immunosuppressive activities of the lankacidin-group antibiotics were studied in mice. seventeen of 29 newly prepared lankacidin-group antibiotics, including 14-derivatives of lankacidin c, lankacidinol, isolankacidinol, and lankacidinol 14-acetate, possessed considerable antitumor activity against ascites 6c3hed/og lymphosarcoma. comparative studies on the antitumor activity of lankacidin c and eight of its derivatives against l1210 leukemia and solid 6c3hed/og lymphosarcoma d ... | 1975 | 1148 |
| the effect of neonatal rat graft-vs-host disease (gvhd) on fc receptor lymphocytes. | the level of fc receptor rosette-forming lymphocytes (fc-rfl) was examined in spleen and lymph node cell suspension from neonatal da and bn rats inoculated within 24 hr of birth with either allogeneic l (experimental) or syngeneic (control) lymphoid cells. in addition, these levels were compared to fetal and neonatal animals that received no injection. the indicator cells (ea) were sheep erythrocytes sensitized with one-half concentration of the highest dilution of rabbit anti-sheep erythrocyte ... | 1976 | 1444 |
| studies on the rumen flagellate neocallimastix frontalis. | the vast increase in the population density of the rumen flagellate neocallimastix frontalis shortly after the host animal has commenced eating is caused by stimulation of a reproductive body on a vegetative phase of the organism to differentiate and liberate the flagellates. the stimulant is a component of the host's diet. the vegetative stage of n. frontalis bears a strong morphological resemblance to that of certain species of aquatic phycomycete fungi, and consists of a reproductive body bor ... | 1975 | 1462 |
| effects of psychotropic drugs on prostaglandin biosynthesis in vitro. | | 1975 | 1505 |
| classification and biological distribution of histamine receptor sub-types. | the distribution and classification of histamine receptors in mammalian and avian tissues have been summarized in tables 1-4. it is evident that histamine receptors are present on a number of morphologically distinct cell types and the proportion of cells bearing h1- and h2-receptors varies not only with the species but also with the cell source. the pharmacological receptors mediating mepyramine-sensitive histamine responses have been defined as h1-receptors. receptors mediating mepyramine-resi ... | 1975 | 1979 |
| partial purification and characterization of post-proline cleaving enzyme: enzymatic inactivation of neurohypophyseal hormones by kidney preparations of various species. | the inactivation of the neurohypophyseal hormones arginine vasopressin and oxytocin, both 14c-labelled in the c-terminal glycine residue, by enzymes present in kidney homogenates of various species has been investigated, and some of the enzymes responsible have been partially purified and characterized. the leu-gly peptide bond of oxytocin is generally most effectively cleaved by kidney homogenates, although with certain species enzymic activity hydrolyzing the pro-leu bond is significant. degra ... | 1976 | 2300 |
| analysis of sheep blood serum haptoglobin. | three types of haptoglobin (hp), differing in the number of bands of hp--hb complex on polyacrylamide gel electrophoregramm are found in sheep blood serum. hpa, hpb and hpc fractions included one, two-three and six-eight bands respectively. a modified procedure for the hpc isolation is described. effects of urea, sodium dodecylsulpate, beta-mercaptoethanol, ph and maleinization on the behaviour of hpc under polyacrylamide gel electrophoresis is studied. the data obtained suggest that the hpc mol ... | 1975 | 2330 |
| pah clearance, sodium excretion, and pah extraction ratio in acidotic near-term lambs treated with hypertonic sodium bicarbonate. | the electrolyte changes and renal hemodynamic adjustment to hypertonic sodium bicarbonate (nahco3) correction of a metabolic acidosis were studied in 4 neonatal lambs and in 2 controls. pah clearance increased from 0.92 to 1.65 ml/min/kg (p less than 0.05), urine flow from 0.37 to 0.61 ml/min/kg (p less than 0.05), and na excretion from 8.4 to 23.7 mueq/min/kg (p less than 0.05) during the nahco3 infusion. these increases were transient and returned to pre-infusion levels following nahco3 infus ... | 1976 | 2340 |
| intermediates in the interconversion of acid and alkaline ferrihemoglobin. structural and kinetic aspects. | the acid-alkaline ph-jump in suspension of crystalline sheep hemoglobin has been studied in the range of 5.95 to 8.94. crystals suspended in 3.8 m cs2so4 show a rapid optical transition of half-time equal to or less than 2 ms. as the ammonia concentration is increased in the cs2so4-suspended crystals, a second optical transition is observed as a pseudo-first-order reaction, with a rate constant of between 10 and 15 s-1. the alkaline-acid ph-jump proceeds through a very rapid shift of the alkalin ... | 1976 | 2464 |
| the role of the capsular polysaccharide in the activation of the alternative pathway by the pneumococcus. | previous studies have shown that when pneumococci are incubated in normal, nonimmune serum, they activate the alternative pathway and opsonically active c3b is fixed to the surface of the organism. other studies have demonstrated that c3-dependent opsonization via the alternative pathway plays a significant role in the nonimmune host's defense against the pneumococcus. the present studies concern the role of the capsular polysaccharide in initiating the activation of the alternative pathway by t ... | 1976 | 2634 |
| possibilities and limitations of uterine contraction inhibiting agents. | | 1976 | 2884 |
| normal blood-gas values in lambs during neonatal development and in adult sheep. | arterial oxygen (pao2) and carbon dioxide (paco2) tensions and ph were determined in 20 lambs during development from two weeks to five months of age and in 34 adult sheep. throughout the period the lambs' mean paco2 (33.1 mm hg) and ph (7.46) values were similar to those determined in the adult sheep (paco232.1 mm hg; ph 7.48). the lamb's mean pao2 increased from 63.3 mm hg at two weeks of age to about 80 mm hg at eight weeks and by 20 weeks was similar to the mean pao2 (88.0 mm hg) of adult sh ... | 1975 | 2962 |
| quantitative removal of carrier ampholytes from protein fractions derived from isoelectric focusing. | | 1975 | 3125 |
| the effects of some polypeptides on the systemic blood pressure of sheep (author's transl). | | 1975 | 3458 |
| influence of protein restriction on immune functions in nzb mice. | the influence of a low protein (6%) diet on the immunologic function of nzb mice was investigated. the low protein intake was associated with decreased weight gain in both male and female nzb mice. the mice fed the low protein diet did not develop splenomegaly, which generally occurs by 7 to 10 months of age in nzb mice fed a normal amount of protein. further, 7- to 10-month-old nzb mice fed the low protein(6%) diet, maintained: 1) more vigorous antibody production to sheep red blood cells; 2) g ... | 1976 | 3605 |
| enzymic hydrolysis of acetylcarnitine in liver from rats, sheep and cows. | 1. the enzymic utilization of o-acetyl-l-carnitine other than via carnitine acetyltransferase (ec 2.3.1.7) was investigated in liver homogenates from rats, sheep and dry cows. 2. an enzymic utilization of o-acetyl-l-carnitine via hydrolysis of the ester bond to yield stoicheiometric quantities of acetate and l-carnitine was demonstrated; 0.55, 0.53 and 0.30mumol of acetyl-l-carnitine were utilized/min per g fresh wt. of liver homogenates from rats, sheep and dry cows respectively. 3. the acetylc ... | 1975 | 4059 |
| characterization of the mannosyl and fucosyl transferases in the ovine anterior pituitary glands. | ovine anterior pituitary glands contain mannosyl- and fucosyl-transferases localized in the microsomes and able to incorporate mannose or fucose as such from gdp-mannose or gdp-fucose into endogenous glycoproteins. the requirements and conditions necessary for maximum activity were investigated. the value of the km is very similar for the two enzyme systems, 3 x 10(-7) m in the case of mannosyl-transferases and 5 x 10(-7) m in the case of fucosyl-transferases. | 1975 | 4162 |
| measurement and comparison of the proliferative and antibody response of neonatal, immature and adult murine spleen cells to t-dependent and t-independent antigens. | | 1976 | 4226 |
| a simple method for the cryopreservation of lymphocytes. retention of specific immune effector functions by frozen-stored cells. | a simple, quick and inexpensive method for cryostorage of lymphocytes is discribed. when injected into appropriate normal recipients, frozen-stored rat and sheep lymphocytes caused gvh and nlt reactions respectively. sheep lymphocytes remained viable after several months storage and functioned satisfactorily as 51cr-labelled target cells in assays for cytotoxic antibodies. lymphocytes, including immunoblasts, drained from sheep efferent lymphatics during immune responses to injected murine p815 ... | 1976 | 4251 |
| equine viral encephalitis. | the most important neurotropic viral infections of the horse are the arthropod-borne encephalitides. these include venezuelan encephalitis (ve), eastern encephalitis (ee) and western encephalitis (we), which are found in the americas, and japanese b encephalitis which occurs in the far east. all the viruses cause encephalitis in man. between 1969 and 1972 an epidemic of ve occurred in central america. in 1971 the disease was reported in texas, where it was brought under control by the vaccinatio ... | 1976 | 4301 |
| a kinetic concepto of lipid transport in ruminants. | summarization of the literature shows a strong correlation between dietary fatty acid intake and total lipid concentration in plasma in lactating cows whereas total milk fat secreted is related to neither of these. in the process of plasma triglyceride removal, chylomicra and very low density lipoproteins are converted to low density lipoproteins. limited kinetic data indicate that the fractional removal rates for chulomicra and very low density lipoproteins are rapid in lactating cows whereas f ... | 1976 | 4477 |
| effect of copper status on brain neurotransmitter metabolism in the lamb. | | 1976 | 4581 |
| the effect of carbonic anhydrase inhibition on bicarbonate reabsorption. | renal reabsorption of bicarbonate was studied in merino ewes during carbonic anhydrase inhibition. bicarbonate reabsorption was directly proportional to plasma bicarbonate concentration. no tubular maximum for bicarbonate was demonstrated. elevation of arterial pco2 or depression of arterial ph caused slight increases in bicarbonate reabsorption. the data suggest that bicarbonate is reabsorbed by 2 distinct processes. the quantitatively more significant process may involve ionic reabsorption of ... | 1976 | 4767 |
| functional assessment of the autotransplanted uterus and ovary in the ewe. | | 1976 | 4803 |
| action of histamine and its receptor blockers on uterine circulation in sheep. | effects of iv and ia administration of histamine and its h1 and h2 blockers (diphenhydramine and metiamide) on systemic arterial pressure, heart rate, and uterine and iliac blood flows were investigated in unanesthetized, chronically instrumented nonpregnant ewes. intravenous histamine produced tachycardia, hypotension, and decreased iliac and uterine blood flows. in contrast, ia injections produced a significant increase in blood flows in these vascular beds which was dose-dependent. evidence i ... | 1976 | 4813 |
| the biological actions of thyrotropin. | | 1975 | 4827 |
| study of cystinaminopeptidase heterogeneity in animal sera by the isoelectric focusing method. | study of the quantitative distribution of cystinaminopeptidase activity in the serum of pregnant and control animals (cow, sheep and rat) by the isoelectric focusing method showed heterogeneity of the cystinaminopeptidase activity of the animals' serum enzymatic system, which was well pronounced in the cow and rat and less pronounced in the sheep. the authors assume from their results that the principal difference between the pregnant and control animals is not the total increase in the level of ... | 1976 | 4848 |
| the effect of mercuric chloride intoxication on urinary psi-glutamyl transpeptidase excretion in the sheep. | the activity of the enzyme psi-glutamyl transpeptidase (psi-gt) was measured in the urine of sheep. in clinically normal animals the mean value was 6-3+/-0-6 mu/ml. in sheep with kidney damage induced by the administration of mercuric chloride there were marked increases in urinary concentrations of psi-gt, in some cases values in excess of 1000 mu/ml were recorded. this enzyme may be of value in the diagnosis of certain forms of renal disease. | 1976 | 4865 |
| the role of aflatoxin metabolism in its toxic lesion. | | 1976 | 4903 |
| influence of various carbohydrate or volatile fatty acid supplements on nitrogen metabolism in wethers. | | 1975 | 4934 |
| studies on the eructation mechanism in sheep. iv. the influence of prolonged fasting on the composition of the eructated rumen gases volume per cent and the (ph) in the ruminoreticulum. | | 1976 | 4935 |
| hormones, second messengers, and the mammalian spermatozoon. | | 1975 | 4967 |
| partial purification and characterization of a reduced nicotinamide adenine dinucleotide phosphate-linked aldehyde reductase from heart. | | 1976 | 5092 |
| endogenous proteolytic activity and constituent polypeptide chains of sheep and pig 19 s thyroglobulin. | porcine and ovine 19-s thyroglobulins prepared from frozen glands in several buffers using slice extraction or homogenization, ammonium sulfate precipitation and deae-cellulose chromatography or sepharose 6b gel filtration were contaminated with protease activity of ph optima 4.5 and 8.6, as shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis. optimum temperatures of autodigestion were 37 degrees c at ph 4.5 and 25 degrees c at ph 8.6. thyroglobulins prepared from unfrozen glands ... | 1976 | 5135 |
| glutathione s-aryltransferase as a model for the glutathione s-transferases. | | 1975 | 5274 |
| interaction of cholera toxin and toxin derivatives with lymphocytes. ii. modulating effects of cholera toxin on in vivo humoral and cellular immune responses. | the in vivo effects of cholera toxin on lymphoid organ structure and function in mice were investigated. it was found that within a day following intravenous injection of 1 mug of toxin, thymus as well as spleen weight decreased but the animals remained healthy. histological studies suggested that the involution of lymphoid organs was due to cell death. injection of cholera toxin into adrenalectomized mice was lethal within 36 h. in these animals no decrease in lymphoid organ weight was noted. t ... | 1976 | 5378 |
| nitrogen metabolism and wethers as affected by dietary protein solubility and amino acid profile. | | 1976 | 5391 |
| trends and innovations in rumen microbiology. | | 1976 | 5779 |
| experimental stimulation of cell-mediated immunity without concomitant stimulation of humoral immunity in graft-versus-host immunosuppressed mice. | the immunosuppressive effect of the graft-versus-host (gvh) reaction was studied in cba x a f1 (caf1) mice which had been rendered immunologically unresponsive by the injection of parental a strain lymphoid cells (gvh mice). suppression of both cell-mediated and humoral immune responses was demonstrated by the prolonged survival of c57bl/6 (b6) skin allografts and by the inability of gvh mice to produce detectable antibody following stimulation with sheep erythrocytes (srbc). appropriate stimula ... | 1976 | 5793 |
| comparative study of the optimum ph value of serum alkaline phosphatase in various species of farm animals. | investigations were carried out on the alkaline phosphatase in the sera of cattle, horses, pigs, sheep, goats, and chickens, the ph value of the buffer used being 9.0-9.8-10.0-10.2-10.6 and 11.0, and the method applied--that of richterich. the ph value at which the serum alkaline phosphatase in the various farm animals and birds was most active was found to vary to a large extent. optimal values for the enzyme's activity usually range as follows: cattle, 10.2; pigs and goats, 10.0; sheep,--10.2; ... | 1975 | 5802 |
| regulatory mast cells. i suppressive action of their products on an in vitro primary immune reaction. | products of mast cell degranulation, as well as histamine and serotonin, were added to a mishell and dutton preparation for in vitro primary immunisation (induction of igm antibody formation) to sheep or horse red blood cells. degranulation products were either obatined beforehand by reacting passively sensitised mast cells with the corresponding antigen (unrelated to or identical with the in vitro immunising antigen) or liberated into the culture medium where mast cells actively sensitised to t ... | 1975 | 5941 |
| clinical experience with the teflo disposable membrane oxygenator. | a simple, inexpensive, highly efficient disposable membrane oxygenator with low priming volume and a microporous membrane recently has become available. animal and clinical investigations of its use have been most satisfactory, and clinical experience now has been extended to include 285 patients. its primary advantage has been the ability to control oxygenation and carbon dioxide separately. disadvantages have included the somewhat increased complexity of the system as compared with bubble oxyg ... | 1976 | 5971 |
| some observations on the nadp+-linked oxidation of methylglyoxal catalysed by 2-oxoaldehyde dehydrogenase. | in the oxidation of methylglyoxal by 2-oxoaldehyde dehydrogenase, the apparent km value for nadp+ was about 2.5 times lower than the corresponding km for nad+; the apparent km values for methylglyoxal and for the amine activator l-2-aminopropan-1-ol, with nadp+ as cofactor, were also different from those obtained with nad+. in the presence of nadp+, the enzyme was not activated by p1, in contrast with the activation of the enzyme when nad+ was used. the significance of the results is discussed. | 1976 | 6014 |
| partial purification and study of gamma-glutamyl transpeptidase from sheep cerebral capillaries. | gamma-glutamyl transpeptidase was purified 53 times from sheep brain cortex capillaries. on gel filtration it appears homogeneous with a m.w. = 350 000. the enzyme is likely a glycoprotein, the properties of which are close to hog kidney gamma-glutamyl transpeptidase ; gamma-glutamyl aminoacid formation is assayed electrophoretically. the results obtained using several aminoacids are in favour of the existence of different units, specific of each group of aminoacids ; together with the data from ... | 1976 | 6068 |
| properties of prostaglandin synthetase of rabbit kidney medulla. | the formation in vitro of prostaglandins e2, d2, and f2alpha from arachidonic acid by rabbit kidney medulla homogenate or microsomal fraction is markedly affected by the composition of the incubation medium employed. optimal biosynthesis is obtained in 0.1 m potassium phosphate buffer, with the optimum ph being 8.0--8.8. under these conditions prostaglandin formation is linear up to arachidonic acid concentration of 30 mum. the initial rate of formation of prostaglandin e2 + prostaglandin d2 is ... | 1976 | 6277 |
| purification and structure of sheep haptoglobin. | | 1976 | 6336 |
| biological expressions of lymphocyte activation. v. characterization of a soluble immune response suppressor (sirs) produced by concanavalin a-activated spleen cells. | supernatant fluids from murine spleen cell cultures incubated with concanavalin a for 48 hr contain a factor(s), soluble immune response suppressor (sirs), which suppresses plaque-forming cell responses to sheep erythrocytes by murine spleen cells in vitro. in the present studies, some of the biochemical and biophysical properties of sirs were investigated. sirs was non-dialysable; the suppressive activity was stable at 56 degrees c for 30 min, but was destroyed by treatment at 70 degrees c for ... | 1976 | 6595 |
| prostglandins--mediators, modulators or metabolites? | | 1976 | 6742 |
| microelectrode measurement of the intracellular ph of mammalian heart cells. | | 1976 | 6917 |
| values of acid-base equilibrium in the blood of various species of domestic animals. | by means of the astrup equilibration method the values of the acid-base balance of the blood were determined in 104 cows, 99 horses, 100 pigs, 15 sheep, 20 goats, and in 101 dogs. the ph values of the blood, the partial pressure of co2, the base excess, the base buffer, the standard bicarbonate, the actual bicarbonate, and the total co2 were processed statistically and are presented in tables. | 1976 | 7040 |
| a new method using p-benzoquinone for coupling antigens and antibodies to marker substances. | a method using p-benzoquinone for coupling antigens and antibodies to enzymes and erythrocytes is described. the method involves the treatment of proteins (or polysaccharides) at ph 6 or 7 with an excess of p-benzoquinone. after removal of the unreacted reagent by gel filtration, the "activated" proteins were coupled at ph 8-9 with enzymes or erythrocytes. biological activities of the proteins were not substantially modified by this treatment since 80-100% of the antigen binding capacity was fou ... | 1976 | 7179 |
| studies on the properties of acid erythrocyte phosphatase in sheep and the isoenzymes of sheep and goat acid erythrocyte phosphatase. | ovine erythrocytic acid phosphatase showed two peaks of activity at ph 5.0 and 5.7 in acetate buffer with p-nitrophenylphosphate as substrate. the enzyme was only slightly inhibited by fluoride and l-phenylalanine, but high concentrations of urea strongly inhibited it. activity of the enzyme was greater in goat erythrocytes than in sheep. by means of starch electrophoresis, three isoenzymes belonging to nine types were separated from the ovine enzymes, while three isoenzymes of five types were p ... | 1975 | 7216 |
| the effects of pentobarbitone and pethidine on foetal breathing movements in sheep. | 1 small doses of pentobarbitone (4 mg/kg i.v.) administered to sheep in the last third of pregancy had little overt effect on the mothers. in the foetus they caused arrest of breathing movements, an alteration in the character of the electrocorticogram and cardiovascular changes which varied with gestational age. 2 in contrast, relatively large doses of pethidine (100-200 mg) admininstered to the mother had no consistent effect on normal foetal breathing movements, though they abolished the foet ... | 1976 | 7337 |
| the carbamate reaction of glycylglycine, plasma, and tissue extracts evaluated by a ph stopped flow apparatus. | we have used a stopped flow rapid reaction ph apparatus to investigate the carbamate equilibrium in glycylglycine solutions and in three biological tissues, human plasma, sheep muscle, and sheep brain, as well as to investigate the kinetics of carbamate formation in glyclyglycine solution and in human plasma. the rapid reaction apparatus was equipped with a ph sensitive glass electrode in order to follow the time course of ph from 0.005 to 100 s after rapid mixing of a solution of amine or prote ... | 1976 | 6479 |
| the effect of microsomal enzyme inducing drugs on reproductive function in the ewe. | | 1975 | 7480 |
| the effects of grass and concentrate diets on the specific activities of some enzymes of hepatic carbohydrate metabolism in sheep. | feeding sheep a concentrate diet compared with grass diets increased the hepatic specific activities of the three glycolytic enzymes studied, and that of glucose-6-phosphate dehydrogenase (ec 1.1.1.49) and reduced the specific activity of d-fructose-i, 6-diphosphate i-phosphohydrolase (ec 3.1.3.11). the specific activities of phosphogluconate dehydrogenase (ec 1.1.1.43) and malate dehydrogenase (decarboxylating) (nadp) (ec 1.1.1.40) were unaffected by diet. | 1976 | 5105 |
| periarteritis in experimental border disease of sheep. ii. morphology and histochemistry of the lesion. | | 1976 | 7578 |
| on the control of enzyme pathways. | | 1976 | 4678 |
| the surface potential of and double-layer interaction force between surfaces characterized by multiple ionizable groups. | | 1976 | 4677 |
| incidence of arbovirus antibodies in bovine, ovine and human sera collected in eastern sicily. | the incidence of antibodies against 9 arboviruses in ovine, bovine and human sera collected in eastern sicily was studied. a high incidence of antibodies was found especially against bhanja virus in all sera and against sandfly fever sicilian in ovine sera only. the incidence was higher than that reported in 1971 for the western part of sicily. these findings suggest an increase in the circulation of arboviruses in this area; the causes are briefly discussed. | 1976 | 7946 |
| genetic differences in lymphoid tissue reactivity during liver regeneration in mice of different lines. | the transfer of lymphocytes together with sheep erythrocytes from partially hepatectomized mice to syngenous lethally irradiated mice (cab and c57bl) increased the number of antibody forming cells in the recipient's spleen. the lymphocytes of cba mice acquired this ability much earlier after the operation (in 4 hours) than those of the c57bl mice (in 17 hours). after the transfer of lymphocytes in the semisyngenous system there was a decrease of antibody forming cells during subsequent recipient ... | 1976 | 8178 |
| metabolism of (4-14c)estrone by sheep erythrocytes around the time of parturition. | the metabolism of 4-14cestrone in vitro by red blood cells of sheep in late pregnancy and after partuirition has been studied. 14cestrone (600 ng) was incubated with 0.5 ml erythrocytes plus 0.5 ml of krebs-ringer phosphate buffer, ph 7.4, for 2 h at 37 degrees c in an atmosphere of air. after incubation, 3hestrogens were added to the incubation medium as internal standards for identification and for correction for procedural losses. metabolites were isolated and purified by chromatography, acet ... | 1976 | 8195 |
| alpha-aminomethylglutarate, a beta-amino analog of glutamate that interacts with glutamine synthetase and the enzymes that catalyze glutathione synthesis. | the glutamate analog, alpha-aminomethylglutaric acid, was synthetized by michael addition of ammonia to 2-methylene glutaronitrile followed by hydrolysis of the intermediate alpha-aminomethylglutaryl nitrile; the analog cyclizes readily on heating to 2-piperidone-5-carboxylic acid. sheep brain glutamine synthetase utilizes one isomer of dl-alpha-aminomethylglutarate at about 10% of the rate with l-glutamate. gamma-glutamylcysteine synthetase uses both isomers of dl-alpha-aminomethylglutarate, pr ... | 1976 | 4441 |
| partial purification and properties of ovine liver echinococcus granulosus protoscolices phospholgucose isomerase. | echinococcus granulosus protoscolex is the actual larval stage of the cestode causing echinococcosis both in man and animals. in the present report, certain properties of phosphoglucose isomerase from the ovine liver e. granulosus protoscolices have been studied and compared with those of the hydatid cyst fluid and the healthy ovine liver enzymes. the protoscolices enzyme prepared in a manner similar to the hydatid cyst fluid and the ovine liver enzymes exhibited the following properties: (1) ph ... | 1976 | 4246 |
| chirality of xylitol-oxidizing enzymes from mammalian liver. | | 1976 | 8339 |
| participation of cyclophosphamide-sensitive t cells in graft-vs-host reactions. | cyclophosphamide (cy)3 is toxic for a population of mouse t cells which is active in graft vs host (gvh) responses. this observation contrasts with previous reports which indicate that treatment of mice with cy regimens, similar to those used in this study, enhances the reactivity of t cells which mediate delayed type hypersensitivity to sheep red blood cells. the gvh-inducing capacity of t cells is not altered by removal of b cells by nylon wool columns. we suggest that cy-sensitive t cells may ... | 1976 | 8571 |
| the haemolytic effect of phallolysin. | phallolysin from the toadstool, amanita phalloides, is a basic protein that causes direct haemolysis of red cells. the dose-response curve is steep; the ph optimum is in the weakly acid range. the rate of haemolysis increases with the concentration of the lysin, the optimal temperature is 20 degrees c. the percentage haemolysis-time curves are s-shaped. haemolysis is of the non-osmotic type. ca2+ is not required but inhibits haemolysis in a concentration-dependent fashion, as do mg2+ and zn2+. t ... | 1976 | 8736 |
| 17 beta-hydroxysteroid dehydrogenase of the sheep ovary : purification, properties and substrate binding site. | sheep ovarian 17 beta hsdh has been purified about 1000 fold to a specific activity of 0.5 iu/mg protein, using deae cellulose chromatography, affinity chromatography on estrone-amino caproate-sepharose and a second deae cellulose chromatography. the molecular weight is 70,000 ; the ph optimum for activity is 9.2 and the energy of activation is 16.5 kcal/mole. the kinetics of the oxidation of estradiol and many analogues have been studied at various concentrations and in the presence of differen ... | 1975 | 4149 |
| possibilities and limits of the intrauterine reanimation (author's transl). | intrauterine reanimation means the removal of acute maternal or fetal distress. beside of maternal shock (traumatic, hoaemorrhagic, supine hypotension syndrome) all acute distress situation are seen during labour. the therapeutical possibilities and premises on the one side, the limits and dangers on the other are discussed. there are the change of position in bed, infusion of low molecular dextrane, o2- breathing, buffering of the mother, infusion of vasodilatators and beta-stimulators to the m ... | 1975 | 3899 |
| general survey of the present distribution of helminths in iran and comparison with those of past years. | in this paper the literature on helminthiasis in iran is reviewed and the distribution, prevalence and intensity of infections caused by helminths are discussed. a comparison of prevalence and intensity of infections was accomplished in the recent 10 years by the author. hookworm infections are found mostly in the north along the caspian sea, but its prevalence is declining. trichostrongylus are found in iran with a high prevalence in central country. schistosoma haematobium is found in one prov ... | 1976 | 8977 |
| circulatory response to atropine in sheep fetus. | atropine was given to the semichronic sheep fetus intravenously. fetal heart rate, fetal perfusion pressure, and carotid blood flow increased. cerebral blood flow was measured with the radioactive microsphere technique. atropine was capable of increasing the cerebral blood flow. elevation of carbon dioxide also caused an increase in cerebral blood flow, adding to the effect of atropine. | 1976 | 8987 |
| comparative rheology of cornea. | | 1976 | 9252 |
| rapid transfer of oxygens from inorganic phosphate to glutamine catalyzed by escherichia coli glutamine synthetase. | measurements are reported on certain isotopic fluxes during the net conversion of glutamine, adp and pi to glutamate, nh3, and atp by escherichia coli glutamine synthetase (adenylylated form, mn2+ activated) in presence of a hexokinase/glucose trap to remove the atp formed during the reaction. the results show that the transfer of oxygens from pi to glutamine is the most rapid of the measured isotopic interchanges, over five oxygens from pi being transferred to glutamine for each glutamate forme ... | 1976 | 9391 |
| a stereochemical method for detection of atp terminal phosphate transfer in enzymatic reactions. glutamine synthetase. | an isotope scrambling method is described for the detection of transient enz:adp:p-x formation from 18oatp in atp-coupled enzyme reactions. the method makes use of torsional symmetry of the newly formed (see article) group in adp. 18 oatp labeled in the betagama bridge oxygen was incubated with enzyme and reversible cleavage of the pbetao -- pgamma bond was detected by the appearance of 18o in the beta nonbridge oxygens of the atp pool. experiments with sheep brain and escherichia coli glutamine ... | 1976 | 9406 |
| fetal blood sampling and gas exchange. | | 1976 | 9427 |
| electrical stimulation of rabbit and lamb carcasses. | | 1976 | 9718 |
| a stable antibody-sensitized erythrocyte diagnostic preparation for detection of rhinovirus type 17 and its antibody. | a diagnostic preparation was obtained by sensitization of formalinized sheep erythrocytes with antibody to rhinovirus type 17. this preparation was agglutinated in the presence of relatively high concentrations of type 17 but not other types of rhinovirus. it could also be used for detection of antibody to rhinovirus type 17 in the antigen neutralization test, the antibody levels determined in this way being similar to those found by conventional virus neutralization tests in cell cultures. | 1976 | 9804 |
| ruminal histamine, lactate and animal performance. | | 1976 | 10270 |
| factors affecting the uptake and metabolism of soluble carbohydrates by the rumen ciliate dasytricha ruminantium isolated from ovine rumen contents by filtration. | a filtration technique is described whereby metabolically-active suspensions of dasytricha ruminantium can be isolated from rumen contents with negligible contamination by bacteria or other protozoa. the effects of environmental factors and of the diurnal cycle of the rumen on the uptake and metabolism of soluble carbohydrates by these isolated cells were examined. the principal contribution of the protozoan metabolic end-products to the host ruminant is the supply of lactic, acetic and butyric ... | 1976 | 10343 |
| purification of an immunosuppressive principle from ehrlich carcinoma ascites. | an immunosuppressive factor was purified from ehrlich carcinoma ascites by the combination of ultrafiltration and sephadex chromatography. the resulting product showed 50% reduction in the number of splenic plaque-forming cells in mice immunized with sheep red blood cells when as low as 50 mug dose was given twice intraperitoneally before erythrocyte injection. the molecular size of the product was between 30,000 and 100,000, and it was relatively heat unstable. | 1975 | 3452 |
| the effect of disulfiram on the aldehyde dehydrogenases of sheep liver. | 1. the effect of disulfiram on the activity of the cytoplasmic and mitochondrial aldehyde dehydrogenases of sheep liver was studied. 2. disulfiram causes an immediate inhibition of the enzyme reaction. the effect on the cytoplasmic enzyme is much greater than on the mitochondrial enzyme. 3. in both cases, the initial partial inhibition is followed by a gradual irreversible loss of activity. 4. the ph-rate profile of the inactivation of the mitochondrial enzyme by disulfiram and the ph-dependence ... | 1975 | 3167 |
| motility of the ruminoreticulum. v. motor activity of the ruminoreticulum, eructation, rumination and hydrogen-ion concentration in the ruminoreticulum in sheep in the course of 24 hours after eating a defined ration. | | 1976 | 10704 |
| ontogeny of tracheal fluid, pulmonary surfactant, and plasma corticoids in the fetal lamb. | we examined fetal plasma corticoids and flow rate, electrolyte composition, and surfactant content of tracheal fluid in chronic experiments with eight fetal lambs. from 120 to 148 days of gestation the rate of fluid production was 4.5 ml/kg per h, and there was no change in mean fluid sodium (147.8 meq/1), chloride (153.1 meq/1), calcium (2.2 mg/100 ml), and ph (6.23). tracheal fluid potassium increased from 4.3 meq/1 at 120-130 days to 8.9 meq/1 at term, while plasma sodium, chloride, calcium, ... | 1975 | 2573 |
| glutamine synthetase and glutamyltransferase in the kidney of man, dog, and rat. | glutamine synthetase (gs) is known to exist in the kidney of the rat, guinea pig, rabbit, and sheep but not in that of the dog, pig, cat, or pigeon. no data is available in man. assay of histologically normal renal tissue obtained in human subjects during surgery for abdominal vascular disease failed to demonstrate significant gs activity. in addition, l-glutamine gamma-glutamyltransferase (gt) activity was also very low. the same results were observed in the dog, in which both gs and gt activit ... | 1976 | 10736 |
| lymphocyte subpopulations. human red blood cell rosettes. | human red blood cells (hrbc) even without prior neuraminidase treatment, could form rosettes with human peripheral blood lymphocytes in vitro. the optimum conditions for forming these rosettes were a ph of 7-0 and a medium with 5% bovine serum albumin (bsa). rosette proportions became much less at a different ph or using lower concentrations of bsa, or replacing bsa with foetal calf sera (fcs) or human sera. rosette formation was also promoted by prior treatment of hrbc or lymphocytes with neura ... | 1975 | 2404 |
| effect of labor inhibitor th 1165 a (partusisten) on cardiovascular parameters, blood gases and ph values of mother and fetus in animal experiments. | | 1975 | 2133 |
| ph-dependent binding of immunoglobulins to intestinal cells of the neonatal rat. | rat and rabbit igg immunoglobulins conjugated to horseradiah peroxidase as a histochemical marker bind at 0 degrees c to the luminal surface of absorptive cells in isolated segments of jejunum from 10-12-day old rats. binding is observed at ph 6.0, near the normal luminal ph of the duodenum and jejunum at this age, but not at ph 7.4. furthermore, no binding occurs when cells are exposed at ph 6.0 to either free peroxidase or peroxidase conjugated to chicken or sheep igg immunoglobulins or bovine ... | 1976 | 11223 |
| effect of dietary fats on ovine adipose tissue metabolism. | the effects of different dietary fats on ovine adipose tissue metabolism have been investigated. six-month old sheep were fed for 6 weeks a control diet or diets supplemented with either tallow or a mixture of sunflower seed oil and soybean oil, treated to protect the fats from hydrolysis and hydrogenation in the rumen, or with maize oil. the rates of fatty acid, glyceride glycerol, and co2 formation were measured in perirenal and subcutaneous adipose tissue slices by following the incorporation ... | 1976 | 11390 |
| study of the amniotic fluid of sheep in the normal course of pregnancy and in abortion. | cytologic, biochemical and immunoelectrophoretic studies were carried out of amniotic fluids in 100 ewes with normal pregnancy and 40 ewes that had miscarried. each month of pregnancy a total of 20 and 8 animals of the two groups, respectively were studied. it was found that the biochemical and metabolic processes taking place in the fetus lead to changes in the amniotic fluids altering the ph value, the alkali reserve, the content of potassium, calcium, phosphorus, alkaline phosphatase as well ... | 1975 | 1894 |
| effect of oxygen saturation on h+ and cl- distribution across the red cell membrane in human and ruminant blood. | alterations of red cell ph (phc) and distribution ratios of h+ (gammah+) and cl (gammacl-) between plasma and red blood cells with oxygenation of blood were studied in human blood (audult and fetal) and ruminant blood (bovine, goat and sheep). the experiments were carried out at a plasma ph of 7.0 to 7.7 and at 37 degrees c. in human blood phc of fully oxygenated blood was 0.035 ph lower than that of fully deoxygenated blood in all ranges of plasma ph studied. in ruminant blood, however, the dif ... | 1976 | 11446 |
| cardiovascular effects of electrical stimulation of the forebrain in the fetal lamb. | modified stereotaxic techniques were applied to fetal lambs during the latter third of gestation. electrical stimulation in the region of the hypothalamus in 10 acute experiments was associated with three patterns of arterial blood pressure and heart rate changes: a pressor-tachycardia response; a pure tachycardia response (abolished by propranolol); and a pure bradycardia response (abolished by atropine). the pressor-tachycardia response was examined in detail in 13 chronic preparations (115-13 ... | 1976 | 1704 |