| the renin-angiotensin system and drinking behavior. | | 1975 | 732 |
| characterization of a caprine herpesvirus. | a virus, which was isolated from kids (capra hircus) affected with a relatively severe generalized infection, was found to contain dna and to have a buoyant density of 1.2820 g/cm3. the virus was sensitive to the action of lipid solvents and trypsin and was rapidly inactivated at ph 3.0 and at temperatures of 50 and 56 c. the virion, an icosahedron consisting of a nucleoid surrounded by a double membrane, measured approximately 135 nm in diameter. on the basis of its chemical and physical proper ... | 1975 | 933 |
| transfer of drugs across the ruminal wall in goats. | the rates at which pentobarbital, salicylate, antipyrine, and quinine were transferred from the rumen of intact, conscious goats were measured. the rates at which the same drugs diffused from the blood plasma (under conditions of constant drug concentration) into the ruminal solution were also evaluated. these compounds were absorbed by simple diffusion, and the rates of transfer were a function of ph of the intraruminal solution. the diffusion of drugs from plasma into the reticulorumen allowed ... | 1975 | 934 |
| water metabolism in the llama. | | 1975 | 1179 |
| effect of liver failure on the ventilatory response to hypoxia in man and the goat. | 1. the ventilatory responses to transient and steady-state hypoxia were measured in ten patients with hepatic cirrhosis and in ten healthy control subjects. successive measurements of these responses were also obtained in six goats before and after the experimental production of liver failure. changes in the effect of steady-state hypoxia on the ventilatory response to hypercapnia were evaluated by successive studies in another goat. 2. in spite of a respiratory alkalosis during liver failure, t ... | 1976 | 2406 |
| igm-mediated, t cell-independent suppression of humoral immunity. | the immunological unreactive state occurring in (t,g)-a-l nonresponder mice after secondary antigen challenge was investigated. syngeneic igm anti-(t,g)-a-l antibody-containing plasma, transferred at the time of the time of primary challenge, induced persistent suppression of autologous specific antibody production. removal of plasma igm with goat anti-mu antisera removed the ability of the plasma to supress. the induction and maintenance of the suppressed state were not different in thymectomiz ... | 1976 | 3607 |
| a kinetic concepto of lipid transport in ruminants. | summarization of the literature shows a strong correlation between dietary fatty acid intake and total lipid concentration in plasma in lactating cows whereas total milk fat secreted is related to neither of these. in the process of plasma triglyceride removal, chylomicra and very low density lipoproteins are converted to low density lipoproteins. limited kinetic data indicate that the fractional removal rates for chulomicra and very low density lipoproteins are rapid in lactating cows whereas f ... | 1976 | 4477 |
| comparative study of the optimum ph value of serum alkaline phosphatase in various species of farm animals. | investigations were carried out on the alkaline phosphatase in the sera of cattle, horses, pigs, sheep, goats, and chickens, the ph value of the buffer used being 9.0-9.8-10.0-10.2-10.6 and 11.0, and the method applied--that of richterich. the ph value at which the serum alkaline phosphatase in the various farm animals and birds was most active was found to vary to a large extent. optimal values for the enzyme's activity usually range as follows: cattle, 10.2; pigs and goats, 10.0; sheep,--10.2; ... | 1975 | 5802 |
| values of acid-base equilibrium in the blood of various species of domestic animals. | by means of the astrup equilibration method the values of the acid-base balance of the blood were determined in 104 cows, 99 horses, 100 pigs, 15 sheep, 20 goats, and in 101 dogs. the ph values of the blood, the partial pressure of co2, the base excess, the base buffer, the standard bicarbonate, the actual bicarbonate, and the total co2 were processed statistically and are presented in tables. | 1976 | 7040 |
| studies on the properties of acid erythrocyte phosphatase in sheep and the isoenzymes of sheep and goat acid erythrocyte phosphatase. | ovine erythrocytic acid phosphatase showed two peaks of activity at ph 5.0 and 5.7 in acetate buffer with p-nitrophenylphosphate as substrate. the enzyme was only slightly inhibited by fluoride and l-phenylalanine, but high concentrations of urea strongly inhibited it. activity of the enzyme was greater in goat erythrocytes than in sheep. by means of starch electrophoresis, three isoenzymes belonging to nine types were separated from the ovine enzymes, while three isoenzymes of five types were p ... | 1975 | 7216 |
| glutamine-stimulated modification and degradation of glutamine synthetase in hepatoma tissue culture cells. | effects of glutamine on glutamine synthetase (gs) activity of hepatoma tissue culture (htc) cells were studied with the aid of a specific goat anti-rat gs serum. immunodiffusion and immunoelectrophoretic tests show that rat liver gs and htc cell gs are immunologically similar but not identical. immunotitrations of htc cell extracts demonstrate that in cells incubated in high concentrations (5 mm) of glutamine, a cross-reacting form of gs with a decreased enzyme-specific activity accumulates. on ... | 1976 | 8212 |
| control of breathing in uremia: ventilatory response to co2 after hemodialysis. | the mechanisms responsible for the transient respiratory alkalosis which follows clinical hemodialysis were evaluated by studying the ventilatory response to carbon dioxide in chronic uremic patients, and in unanesthetized normal and chronic uremic goats. a significant increase in sensitivity to co2 was found in acidotic uremic patients immediately (within 30 min) following hemodialysis (p less than 0.01). sensitivity to co2 returned to the predialysis value within 24 h. lung volume and maximal ... | 1976 | 8421 |
| utilization of volatile fatty acids in ruminants. 8. acetate activation in mammary tissue. | | 1976 | 9435 |
| effect of oxygen saturation on h+ and cl- distribution across the red cell membrane in human and ruminant blood. | alterations of red cell ph (phc) and distribution ratios of h+ (gammah+) and cl (gammacl-) between plasma and red blood cells with oxygenation of blood were studied in human blood (audult and fetal) and ruminant blood (bovine, goat and sheep). the experiments were carried out at a plasma ph of 7.0 to 7.7 and at 37 degrees c. in human blood phc of fully oxygenated blood was 0.035 ph lower than that of fully deoxygenated blood in all ranges of plasma ph studied. in ruminant blood, however, the dif ... | 1976 | 11446 |
| the evolution of plants and animals under domestication: the contribution of studies at the molecular level. | protein molecules are essential catalysts in life processes and also form much of the substance of living material. their three dimensional structures determine their biological function. their biosynthesis is primarily determined by arrays of nucleic acid macromolecules (dna and rna), and the amino acid sequences that constitute their long spatially organized peptide-chain molecules reflect at one remove this dna coding system, and thus record a step-by-step history of some of the viable geneti ... | 1976 | 11513 |
| changes in the blood pictureof the red goat of maradi as a function of its gastrointestinal parasitism. | numerous "chèvres de maradi" are bred in republique du niger (cap. niamey), 2 - 10(+6) numbered in 1973. this rustic ruminant is often very parasitized by intestinal nematodes and sporozoa. the most frequent genera are bunostomum (55%), trichostrongylus (40%), strongyloides (27%), oesophagostomum and haemonchus (20%), sometimes moniezia or stilesia, coccidiosis being endemic and very pathogenic, eimeria (70%). polyparasitism is a "modus vivendi" between the host and these various parasites. all ... | 1976 | 11658 |
| isolation and characterization of low and high affinity goat antibodies directed to single antigenic sites on human hemoglobin. | | 1976 | 13037 |
| mouse lymphocytes with and without surface immunoglobulin: preparative scale separation in polystyrene tissue culture dishes coated with specifically purified anti-immunoglobulin. | mouse spleen cells could be preparatively separated into immunoglobulin positive (ig+) and immunoglobulin-netative (ig-)populations by incubating as many as 2 x 10(8) cells per 100 mm diameter petri plate coated with specifically purified goat anti-mouse immunoglobulin. the non-adherent population was 95% or more ig-, and possessed graft versus host and cytotoxic effector activities, as would be expected for t cells. they could also give a mixed lymphocyte reaction and generate cytotoxic effecto ... | 1977 | 15036 |
| arrhythmic doses of epinephrine and dopamine during halothane, enflurane, methoxyflurane, and fluroxene anesthesia in goats. | the cardiac arrhythmicity of epinephrine and dopamine was compared in awake goats and during approximate equivalent levels of halothane, enflurane, methoxyflurane, and fluroxene anesthesia. the arrhythmic threshold dose for epinephrine and dopamine was significantly (p less than 0.05) reduced during halothane anesthesia when compared to values determined in awake animals. enflurane anesthesia had no significant affect on the arrhythmic threshold dose for either catecholamine. however, methoxyflu ... | 1977 | 15472 |
| fasciola hepatica and fasciola gigantica: total cholinesterase, characteristics, and effects of specific inhibitors. | | 1977 | 16762 |
| formation of glutamine and activities of glutamine synthetase in goats. | | 1977 | 17029 |
| the pharmacology of experimental myopathies. | | 1977 | 17357 |
| effects of increased intracranial pressure on pulmonary vascular resistance of fetal and neonatal goats. | the effects of increased intracranial pressure on the pulmonary circulation were investigated in fetal and neonatal goats. pulmonary vascular resistance and systemic arterial pressure increased with elevation of intracranial pressure in neonatal animals. alpha-adrenergic blockade completely eliminated both of these responses. the response of the fetal pulmonary circulation was unlike that seen in the postnatal animal. although there was a slight elevation of pulmonary vascular resistance initial ... | 1977 | 18019 |
| new techniques to measure blood cholinesterase activity in domesticated animals. | a macromethod and a semimicromethod were developed to measure erythrocyte acetylcholinesterase activity in cattle, sheep, goats, horses, dogs, and swine, and to measure plasma cholinesterase activity in horses, dogs, and swine. comparison of the 2 methods with erythrocytes of sheep, cattle, goats, and horses indicated both methods gave similar results. they can be done in a shorter time and are more sensitive than michel's method. normal deltaph values per minutes, with standard deviations for b ... | 1977 | 18075 |
| distribution of beta-n-acetylglucosaminidase, hyaluronoglucosaminidase and acrosin in buffalo and goat spermatozoa. | the distribution of beta-n-acetylglucosaminidase, hyaluronoglucosaminidase and acrosin in buffalo and goat sperm acrosomes was studied. the three hydrolases were found to occur in soluble and bound forms. in the bound form, they were associated with the denuded sperm and were maximally solubilized at ph 3.0. the possible role of beta-n-acetylglucosaminidase in fertilization is discussed. | 1977 | 18396 |
| nature of the rabbit acrosome reaction-inducing activity of follicular fluid. | follicular fluid samples from rabbits, cats, pigs, women and cows had acrosome reaction-inducing activity (aria) on rabbit spermatozoa as determined by differential staining after incubation with these fluids. activity was retained after dialysis and at least 50% was found to be labile when heated to 56 degrees c for as little as 20 min. the induction of the acrosome reaction by bovine follicular fluid showed a dependence on the concentration of follicular fluid and spermatozoa and on calcium io ... | 1977 | 18603 |
| fusiform erythrocytes in angora goats resembling sickle cells: influence of temperature, ph, and oxygenation on cell shape. | | 1977 | 18973 |
| reaction of selenium with immunoglobulin molecules. | radioactive selenite reacts with purified human and goat immunoglobulins at acidic and neutral ph. the antigenic properties of the immunoglobulins are retained during the selenium labelling as shown by immunoelectrophoresis and autoradiography. pepsin digests of 75se-labelled igg possess 75se both in the (fab')2 fraction and in the low molecular weight peptides derived from the fc domains. alpha-1-acid glycoprotein, ribonuclease, and lysozyme are also labelled by this procedure. enhancement of 7 ... | 1977 | 19084 |
| cerebral blood flow and vascular reactivity after removal of the superior cervical sympathetic ganglion in the goat. | | 1977 | 19161 |
| studies on the physico-chemical properties of alhagain. | the protease isolated jawasee shrub was found to hydrolyze egg albumin, casein, haemoglobin and gelatin optimally near neutral ph. fibrin, bovin serum albumin, skin albumin and skin mucoids were hydrolyzed at slightly alkaline ph, while skin globulins were hydrolyzed at slightly acidic ph. the enzyme had no effect of fibrous collagen. the optimum conditions for the hydrolysis of 50 mg of egg albumin were found to be 50 mg of alhagain at ph 6.0 and 45 degrees c for 30 minutes. a km value of 4.4 x ... | 1977 | 21147 |
| adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin a, wheat germ agglutinin and goat anti-human immunoglobulin g on cell surfaces at the electron microscopic level: a new method, theory and application. | a method is described for the adsorption of selected macromolecules to colloidal gold which is then used as an electron dense marker for the indirect detection of specific cell surface molecules. membrane bound concanavalin a, which binds specific sugars on horseradish peroxidase, and wheat germ agglutinin, which binds specific sugars on ovomucoid are detected indirectly with gold labeled horseradish peroxidase and ovomucoid, respectively. goat anti-human igm on blood lymphocytes is detected wit ... | 1977 | 21217 |
| acid elution of blood group antibodies from intact erythrocytes. | human igm and igg antibodies against blood group antigens (a, b, d, c, c, e, e, fya, k), autoantibodies and mouse igm and igg antibodies against sheep erythrocytes have been eluted from intact human and sheep red cells by glycine-hcl buffer, ph 3.0. the yield of human antibodies was higher with acid than with heat and ether elution, and the contamination of hemoglobin in the eluate was negligible. the acid elution method is very simple and rapid and, therefore, highly suitable for experimental a ... | 1977 | 21486 |
| a change in the internal affinity of lk goat red-cell sodium pumps induced by high ph. | the k inhibition of ouabain-sensitive atpase activity of lk goat red cell membranes is greatly reduced at high ph. this effect is reversible, and specific, since the apparent affinities for atp, ouabain or external k do not alter. anti-l-treated membranes show a similar alkali-induced affinity change, but have a lower ph optimum. | 1977 | 21689 |
| preparation of 125i polypeptide hormones for radioimmunoassay using glucose oxidase with latoperoxidase. | | 1977 | 22011 |
| establishment of the hosts of bloodsucking mosquitoes in abkhazia. 3. fertility of females of mass mosquito species feeding on warm-blooded and cold-blooded animals. | | 1977 | 22029 |
| acid-base and metabolic factors: state of the art. | | 1978 | 23252 |
| is brain ecf h+ an important drive to breathe in man? | | 1978 | 23255 |
| some clinical aspects of caprine reproduction. | several clinical reproductive problems in the female dairy goat are considered, including estrus detection, artificial insemination, pregnancy diagnosis, abortions, and periparturient difficulties. sexual behavior of the buck, onset of puberty, techniques for semen collection and evaluation, the production of teaser animals, and methods of castration are also discussed. recognition of the intersex goat, which may have a masculine, feminine, or intermediate phenotype, is necessary in herds where ... | 1978 | 24527 |
| cytochemical demonstration of esterases in peripheral blood leukocytes. | cytochemical methods for alpha naphthyl acetate esterase and chloroacetate esterase have been used to identify human monocytes and granulocytes. in this study, a standard procedure for staining alpha naphthyl acetate and chloroacetate esterase activities was modified by extending the range of ph of the incubation mixture and the duration of staining and was applied to cat, dog, goat, guinea pig, hamster, human, pig, rabbit, rat, and sheep leukocytes. the results for both enzymes showed (1) incub ... | 1978 | 25603 |
| the electrical potential difference and impedance between csf and blood in unanesthetized man. | the electrical potential difference (pd) between cerebrospinal fluid (csf) and blood has been studied extensively in animals. to obtain data from man the pd and impedance were recorded from the lumbar subarachnoid space of thirteen unanaesthetized patients with a presumably normal blood--brain barrier. the spontaneous pd ranged from 1 mv to 5 mv, csf positive to blood. hyperventilation and co2-inhalation changed the pd with a mean slope of deltapd/deltapha of --4.16 mv/ph. the csf-blood pd in un ... | 1978 | 26966 |
| seroepidemiological investigations in domestic ruminants from egypt, somalia and jordan for the demonstration of complement fixing antibodies against rickettsia and chlamydia (author's transl). | 1450 random serum samples of domestic ruminants from egypt, somalia and jordan were investigated for complement fixing antibodies against rickettsia and chlamydia. between 1.5 and 3.4% of the samples from the animals investigated had antibodies against the rmsf-group of rickettsia, with exception of the sera from somalian cattle and sheep from jordan. antibodies against rickettsia of the typhus-group were found in 4 cattle and 1 goat from jordan and 2 sheep from egypt; by agglutination test with ... | 1978 | 28651 |
| reproduction and breeding of goats. | reproduction and genetics of the goat are reviewed with a view of increasing their contribution to mankind. the goat contributes most in tropical regions (within 30 degrees of the equator). the most important product from the goat is milk with meat a close second. other products are minor. reproductive rate is a problem only with the angora goat, but increased reproduction with any type of goat would contribute to improved efficiency. also, a knowledge of the reproductive phenomenon is necessary ... | 1978 | 29915 |
| redistribution of hydrogen ion and chloride, and water shift across the red cell membrane of human and ruminant blood with changes in oxygen saturation. | | 1978 | 30065 |
| on the heterogeneity of catalase from goat liver. purification and characterization. | | 1978 | 32178 |
| studies on the glycosidases of semen: purification and properties of alpha-d-mannopyranosidase from goat seminal plasma. | alpha d-mannosidase activity in goat semen was observed to be distributed in sperm and seminal plasma. in sperm the enzyme, present in soluble and bound forms, was located within the acrosome. the bound enzyme was associated with the denuded sperm. seminal plasma alpha-mannosidase was purified 100-fold and the final preparation was shown to be homogeneous by polyacrylamide and sds gel electrophoresis and on isoelectric focusing. the molecular weight of the enzyme, determined by gel filtration an ... | 1978 | 32482 |
| localization of the site of adenylylation of glutamine synthetase by electron microscopy of an enzyme-antibody complex. | antibodies to the nucleosidel,n(6)-ethenoadenosine have been used to localize the site of adenylylation of the glutamine synthetase [l-glutamate:ammonia ligase (adp-forming), ec 6.3.1.2] of escherichia coli. antibodies were induced in rabbits by injection of a bovine albumin-ethenoadenosine conjugate. the resulting antisera strongly bound ethenoadenosine, its 5'-nucleotide, or protein conjugates of the nucleoside; little or no crossreaction was seen to adenosine, amp, or the protein carrier. eth ... | 1978 | 32536 |
| [changes in mammary tissue lipid metabolism in the goats during the first 2 months of lactation]. | 5 french alpine goats were studied after normal or premature parturition. mammary tissue acetyl-c oa carboxylase, glucose-6-phosphate dehydrogenase, malic enzyme and lipoprotein lipase (lpl) activities varied in parallel with milk fat secretion from the 3rd to the 9th week of lactation. variations of mammary lpl activity and of long chain fatty acid secretion were positively highly correlated during that period. in goats with normal parturition, lipogenic activities seemed to reach a maximum lev ... | 1978 | 32969 |
| proceedings of the international embryo transfer society. fifth annual conference - 14-16 january, 1979. | | 1979 | 33864 |
| repulsive van der waals forces. i. complete dissociation of antigen-antibody complexes by means of negative van der waals forces. | | 1979 | 33892 |
| [5th international symposium on infectious diseases in sheep and goats. tunis, 20-22 november 1978]. | | 1979 | 34245 |
| fetal and neonatal pulmonary circulation. | | 1979 | 35091 |
| the role of prostaglandins in the central nervous system. | | 1979 | 35094 |
| simultaneous localization of lhrh and catecholamines in rat hypothalamus. | the pap unlabelled antibody enzyme method of sternberger was used for the histochemical demonstration of lhrh and the catecholamine synthesizing enzyme, tyrosine hydroxylase (th) in the hypothalamus of the adult male and pregnant female rat. the sections for light and electron microscopy were serially treated with normal goat serum, lhrh antiserum and/or th antiserum, goat anti-rabbit igg, pap complex and 3,3'-diaminobenzidine (dab) or 4-cl-1-naphtol. lhrh-positive cell bodies were discernible i ... | 1979 | 35506 |
| [sequence of the changes in the weight percentage of the right heart ventricle in various animal populations under alpine conditions]. | | 1979 | 35906 |
| synthesis of two classes of antibody, gammam and gammag or gammam and gammaa, by identical cells. amplification of the antibody response to pneumococcal polysaccharide type iii. | class-specific plaque-forming cell (pfc) (gammam, gamma1, gamma2, and gammaa) responses to type iii pneumococcal polysaccharide (sss-iii) were studied in balb/c x c57bl/6f1 (cbf1) mice with and without induction of an allogeneic effect. gamma1, gamma2, and gammaa pfc were detected in two ways: (a) with the sequential development of the assay slides, first for direct (gammam)pfc followed by incubation with class-specific antiimmunoglobulin and complement for the development of additional gamma1, ... | 1979 | 36442 |
| improvement of the glucose oxidase immunoenzyme technic. use of a tetrazolium whose formazan is stable without heavey metal chelation. | glucose oxidase immunoenzymatic localization provides a simple way to show antigens in mammalian tissues, with no need for the quenching of endogenous nonspecific staining. the method is useful for the demonstration of many antigens in formalin-fixed, paraffin-embedded tissues. improvement of this technic by the use of p-nitro blue tetrazolium chloride (nbt) as the disclosing reagent provides a stable, finely grained localization not possible with the previously used thiazolyl blue (mtt). the de ... | 1979 | 36746 |
| fetal breathing: a review. | the history, physiology, pharmacology, and clinical application of fetal breathing are reviewed. early optimism that measurements of fetal breathing would aid in perinatal management decisions has remained unfulfilled. problems to be overcome include standardization of technique and measurement parameters and allowance for physiologic variations. recent research developments indicate that the likely role of fetal breathing in perinatal medicine will be in conjunction with other established tests ... | 1979 | 36761 |
| biochemical processes involved in ferrihemoglobin formation by monohydroxyaniline derivatives in erythrocytes of birds and mammals. | | 1979 | 37027 |
| divergent results in radial immunodiffusion. iii. isolation and specificity test of class-specific antibodies. | a method is described for the isolation of antibodies specific for immunoglobulin class determinants from antisera containing antibodies both for the immunoglobulin class and for its subclasses, using affinity chromatography. the model system used is bovine igg with its subclasses igg1 and igg2, together with antisera raised in goats and sheep. the specificity of the isolated class-specific antibodies was demonstrated by double diffusion (di) and radial immunodiffusion (rid). the levels of total ... | 1979 | 37277 |
| [metabolic activity of adipose tissue in the goat during gestation and at the beginning of lactation]. | in 5 french alpine goats, omental adipose tissue acetyl-coa carboxylase, glucose-6-phosphate deshydrogenase, malic enzyme and lipoprotein lipase activities significantly decreased during the third month of gestation, whereas plasma non-esterified fatty acid and triacyglycerol contents increased. this probably reflects an early decreasing rate of adipose tissue anabolism during gestation in the goat. at the third week of lactation, anabolic activities relative to dna content of adipose tissue wer ... | 1978 | 38015 |
| the development of the binocular depth cells in the secondary visual cortex of the lamb. | in most respects, the response properties of cells in the secondary visual cortex of the newborn lamb were indistinguishable from those in the adult. the cells were sharply selective to orientation; the orientation preferences were the same in each eye, and they varied systematically as the electrode penetrated the cortex. the receptive-field organization did not differ noticeably from that in adults, and complex, hypercomplex, and a few simple cells were all observed. the ocular dominance distr ... | 1979 | 38458 |
| lipid metabolism in the mammary gland of ruminant animals. | | 1979 | 38463 |
| in vitro binding of trypanosoma congolense to erythrocytes. | trypanosoma congolense broden, an intravascular parasite, binds to vessel walls and erythrocytes of infected hosts. in an attempt to characterize t. congolense adhesion to host cells, an in vitro assay was devised. it was shown in the in vitro experiments that t. congolense binds to bovine, sheep, and goat erythrocytes, but not always to erythrocytes of rats, mice, rabbits, horses or humans. only the anterior part of live trypanosomes adheres to erythrocytes, and the attachment site on the trypa ... | 1979 | 39167 |
| an evaluation of a continuous tissue ph monitor in the fetal and neonatal goat. | the validity of continuous tissue ph measurements was established in the fetal and neonatal goat model. simultaneous determinations of tissue, arterial, and venous ph were done during alterations in acid-base status. a highly significant correlation was found between tissue and blood ph levels (r = 0.89, p less than 0.001). these data confirm the accuracy of the tissue ph instrumentation in predicting core blood ph status and support the potential usefulness of the instrumentation in perinatal m ... | 1979 | 39453 |
| antigen-antibody interactions and the anomalous kinetics of arylsulfatase a. | antibodies against homogeneous rabbit liver arylsulfatase a (aryl-sulfatase sulfohydrolase, ec 3.1.6.1) were produced in a goat and the effects of these antibodies on the kinetic parameters of the enzyme have been studied. the results indicate that the binding of antibody to the enzyme does not alter the enzyme active site, since km and -ki values are unaffected. however, a small reduction in the enzyme activity was observed as the result of a reduction of v in the enzyme-antibody complex. the b ... | 1979 | 39607 |
| [the effect of large burns in ruminants on the edibility of meat]. | the effect of non-contact burns was studied on a model of a slaughter ruminant. the study included the examination of the penetration of germs into the blood stream and into the meat and of the main changes characterizing the ripening of the obtained meat. the tests were conducted with two groups of animals, killed 1) at the beginning of the development of the infection process in the burn, 2) in a health state in which the prognosis was unfavourable quo ad vitam. it was found on the basis of ha ... | 1979 | 40336 |
| humoral factor that specifically regulates factor x levels in rabbits (coagulopoietin-x). | a heat-stable humoral substance (coagulopoietin-x) is present in rabbits partially depleted of factor x, which is capable of raising factor x levels when injected into recipient rabbits. rabbits were partially depleted of factor x by slow infusion of a globulin fraction of goat anti-rabbit factor x antibody. this resulted in the reduction of factor x to 40--50% of normal at 1 h and 60--70% of normal at 6 h. no effect was noted on levels of factors ii, v, or vii. plasma from these animals, when i ... | 1979 | 41003 |
| autoimmune response to acetylcholine receptors in myasthenia gravis and its animal model. | | 1979 | 41416 |
| jos virus infection in domestic animals and primates. pathogenicity and immunological studies. | three species of domestic animals (rabbits, sheep and goats) and two species of primates erythrocebus patas monkeys and baboon (papio anubis) were infected with jos virus. none of the infected animals developed viraemia or overt clinical disease. complement-fixing and neutralising antibodies were demonstrated in sera of infected animals. | 1979 | 41441 |
| simple purification of goat igg1 and igg2 subclasses by chromatography on protein a-sepharose at various ph. | | 1979 | 42614 |
| composition of cerebral fluids in goats adapted to high altitude. | we explored the ionic composition of cerebral interstitial fluid (cisf) in six unanesthetized goats at sea level (sl) and again after 5 days at a simulated high altitude (ha) of 4,300 m. by measuring net transependymal fluxes of hco3-, cl-, and lactate during ventriculocisternal perfusions with lactate-free artificial cerebrospinal fluid (csf) with various [hco3-] and [cl-], we determined [hco3-] and [cl-] in the inflowing perfusate that produced zero flux, which are estimates of the concentrati ... | 1979 | 42634 |
| ph gradient elution of human igg1, igg2 and igg4 from protein a-sepharose. | pooled human serum having a normal igg subclass content was chromatographed on a column of protein a-sepharose. the immunoglobulins that bound to the column at ph 7.0 were eluted with a ph gradient generated by 3 equal volumes of citrate/phosphate buffer at ph 5.0, 4.5 and 2.2. the elution pattern consisted of two major overlapping peaks centered 0.4 ph units apart in the ph gradient; on average the first peak centered at ph 4.7, and the second centered at ph 4.3. upon second passage of each com ... | 1979 | 42659 |
| [seroepidemiologic studies on reovirus infections of man, domestic and wild animals in tanzania (author's transl)]. | 2238 sera of bovines, 95 of goats, 251 of antelopes (various species), 143 of zebras and 11 of warthogs collected in tanzania as well as 811 sera of men and females of the city and the region of dar es salaam were tested for haemagglutination inhibiting antibodies (ab) to reovirus serotypes (st) 1, 2 and 3. ab to st 1 resp. 2 could be detected in 24--39% of bovines, goats, antelopes and zebras, and in warthogs to 64%. in human beings the positive percentage was 60%. ab to st 3 were most prevalen ... | 1979 | 43091 |
| hepatotoxicity of chloramphenicol in normal goats by the assay of serum enzyme activity. | | 1979 | 43639 |
| [enzyme immunoassay for the detection and identification of dengue viruses from infected mosquitoes (author's transl)]. | the authors deal with a "double sandwich" enzyme immunoassay for the detection and identification of dengue viruses 1, 2 and 4. they use ground glass-beads (7 mm diameter) coated with human heterospecific anti-dengue antibodies, to bind viruses obtained from infected mosquitoes (toxorhynchites amboinensis). the mouse antibodies bound to the viruses coated on the beads are revealed by anti-mouse 7s immunoglobulins labelled with peroxydase. the method is practical, sensitive and confident. the sta ... | 1979 | 43691 |
| quantitative estimation of ammonia absorption by goats fed high starch diets with urea supplement. | | 1979 | 43697 |
| central chemical regulation of respiration in term newborn. | the role of the medullary h+-sensitive chemoreceptors on the drive of breathing was studied in 10 unanesthetized newborn animals (8 lambs and 2 kids). the experiment consisted of sequential measurements of ventilation (ve) during a progressive change in the arterial ph (pha) and in the ph of the cisternal cerebrospinal fluid (phcsf), induced by intravenous infusion of hydrochloric acid (hcl) followed after an 8-h steady state of acidosis by rapid bicarbonate [hco3-] infusion. it is shown that a ... | 1979 | 43860 |
| the rift valley fever epizootic in egypt 1977-78. 2. ecological and entomological studies. | epidemiological factors related to the introduction, spread and maintenance of rift valley fever (rvf) virus were studied during the 1977-78 epizootic in egypt, culex pipiens is the most ubiquitous and prevalent mosquito species in the nile valley and delta. isolation of rvf virus from unengorged c. pipiens, and demonstration of laboratory transmission of the virus by this species, strongly implicate it as the chief vector in egypt. virus transmission to man also occurs by contamination when han ... | 1979 | 44038 |
| detection of anticapsular antibodies to bacteroides asaccharolyticus in serum from rabbits and humans by use of an enzyme-linked immunosorbent assay. | a sensitive serologic test, the enzyme-linked immunosorbent assay (elisa), was used to detect serum igg antibodies directed specifically to a capsular antigen of bacteroides asaccharolyticus (previously known as bacteroides melaninogenicus subspecies asaccharolyticus). anticapsular igg was measured in 30 specimens of rabbit serum after the animals were immunized with whole b. asaccharolyticus, the two subspecies of b. melaninogenicus, and several other bacterial species. species-specific anticap ... | 1979 | 44309 |
| pathogenicity of ureaplasmas for animals and man. | since their original isolation from the genital tract of man, ureaplasmas, previously termed t-strain mycoplasmas, have been isolated from a variety of animal species. under experimental conditions they have been shown to cause mastitis in cattle, goats and mice, and observations made on naturally-occurring bovine pneumonia, as well as the results of experimental inoculation, suggest that ureaplasmas are responsible for a portion of bovine cuffing pneumonia. ureaplasmas have been isolated from t ... | 1979 | 44609 |
| pathogenicity of the subspecies mycoides of mycoplasma mycoides for cattle, sheep and goats. | recent work has shown that strains classified as m. mycoides subsp. mycoides may be separated into 2 types according to their growth rate and their behaviour in certain biochemical tests. the large colony (lc) types, most of which are from goats, are pathogenic for sheep and goats but apparently not for cattle. the small colony (sc) types include the classical contagious bovine pleuropneumonia (cbpp) strains from cattle and four strains from goats. these sc types are potentially pathogenic for c ... | 1979 | 44610 |
| responses of pulmonary and systemic circulations of perinatal goats to prostaglandin f2 alpha. | | 1979 | 44688 |
| the complement system in bullous pemphigoid. ii. immunofluorescent evidence for both classical and alternate-pathway activation. | | 1975 | 45850 |
| serological analysis of human deoxyribonucleic acid polymerases. preparation and properties of antiserum to deoxyribonucleic acid polymerase i from human lymphoid cells. | the preparation and properties of an antiserum to human dna polymerase i (6 to 8 s) are described. care was taken in the purification of the antigen to remove certain other dna polymerases found in human cells. an incubation of antigen and antiserum lasting about 48 hours is necessary to achieve maximal inhibition. about 1 mug of the antipolymerase immunoglobulin g, prepared in rats, neutralizes 60% of the activity present in 54 ng of the enzyme. tritrations varying both antiserum and enzyme dem ... | 1975 | 46229 |
| dissociation of the immunologic release of histamine and slow reacting substance of anaphylaxis from human lung using cytochalasins a and b-1. | cytochalasins a and b at low doses resulted in enhancement of the antigen-induced and anti-ige-mediated release of histamine from human lung fragments in vitro. both compounds appeared to effect a dose-dependent inhibition of the antigen-induced release of slow reacting substance of anaphylaxis. although the effects observed with cytochalasin b were at least partially reversible, the effects of cytochalasin a could not be readily reversed. the cytochalasins appeared to be more effective when pre ... | 1975 | 46240 |
| association of a beta2-microglobulin-like subunit with h-2 and tl alloantigens on murine thymocytes. | | 1975 | 46243 |
| identification of a colon-specific antigen (csa) in normal and neoplastic tissues. | an antigen has been isolated from a human signet-ring cell carcinoma serially growing in hamsters, gw-39, by saline, pca, or phenol extraction, and has been found immunologically identical to a similarly extracted substance in normal human or hamster colon. no other hamster or human tissues or cells were found to contain this antigen, for which reason we have termed it colon-specific antigen, or csa. csa has been found to be distinct from the major blood group-specific antigens and from othercol ... | 1975 | 46258 |
| complement-mediated release of histamine from human leukocytes. | activation of either the alternative or classical pathway of complement generated a factor which induced release of histamine from both non-allergic and allergic human basophils. this factor probably is derived from the complement system since 1) its formation was associated with loss of c3 activity in human serum, 2) chemotactic factor, probably also a complement product, was generated simultaneously, 3) heat inactivation blocked its formation, 4) anti-c3 and anti-c5 blocked formation of the fa ... | 1975 | 46261 |
| identification of actin-like proteins in alcoholic hyaline by immunofluorescence. | immunofluorescence studies on human liver from alcoholic patients demonstrate that alcoholic hyaline (mallory body) binds immune sera containing anti-actin antibodies. these findings, establishing that alcoholic hyaline is endowed with contractile proteins, strongly support the actin microfilament nature of the mallory body. | 1975 | 46331 |
| alcian blue-tartrazine; a simple differential stain for the fundic gland cells of mammalian stomach. | | 1975 | 46639 |
| nephropathy associated with sickle cell anemia: an autologous immune complex nephritis. i. studies on nature of glomerular-bound antibody and antigen identification in a patient with sickle cell disease and immune deposit glomerulonephritis. | the nature of the glomerular-bound antibody and the putative antigen was investigated in one of the patients with sickle cell disease and immune deposit membranoproliferative glomerulonephritis by immunohistologic and glomerular antibody elution. renal proximal tubular epithelial antigen was localized in association with immunoglobulins g (igg), m (igm), clq fraction of the first component of complement (clq) and the third component of complement (c3) in a granular pattern along the glomerular b ... | 1975 | 46705 |
| neutrophil alkaline phosphatase: comparison of enzymes from normal subjects and patients with polycythemia vera and chronic myelogenous leukemia. | to determine whether decreased alkaline phosphatase activity in the granules from neutrophils of patients with chronic myelogenous leukemia (cml) was due to an absence of enzyme or the production of defective enzyme, we compared the immunologic properties of granule alkaline phosphatase derived from patients with cml with that of normal subjects and patients with polycythemia vera (prv). antisera prepared in rabbits against granule alkaline phosphatase purified from the neutrophils of a patient ... | 1975 | 46759 |
| superficial antigens of campylobacter (vibrio) fetus: characterization of antiphagocytic component. | a glycoprotein responsible for the antiphagocytic properties of campylobacter fetus has been identified by comparing cells of a wild-type strain with those of a mutant lacking this substance. the antiphagocytic component is demonstrable through electron microscopy as a discrete, negatively charged structure on the periphery of the cell. clt is readily removed from the cell by mild extraction procedures and contributes to the inagglutinability in o antiserum normally displayed by c. fetus. cells ... | 1975 | 46843 |
| half-lives of two types of rat homocytotropic antibodies in circulation and in the skin. | the half-lives of two classes of rat immunoglobulins with homocytotropic properties, i.e., ige and igg2a, in the circulating blood and in the skin were studied. the catabolism of both normal (reaginic) and pathological (myeloma) ige proteins in circulation was found to be extremely rapid with a half-life of 12 h. in contrast, the half-life of ige antibody in the homologous skin was calculated to be 7.4 days. on the other hand, the half-life of igg2a in circulation was about 5 days regardless of ... | 1975 | 46847 |
| studies in immunodermatology. vi. if studies of autoantibodies to the stratum corneum and of in vivo fixed igg in stratum corneum of psoriatic lesions. | indirect immunofluorescent (if) tests on sections of normal human skin reveal the presence of antibodies to the stratum corneum in most normal human sera. sera absorbed with hyperkeratotic scales gave negative reactions. these reactions appeared to correspond to those of the stratum corneum antibodies which were first detected by krough and tonder by immune adherence, though this remains to be documented since the conjugate alone stained the stratum granulosum in a pattern comparable to that of ... | 1975 | 46852 |
| observations on rabbit thymocytes and peripheral t cells. i. anomalous results in the mixed antiglobulin reaction caused by non-specific adsorption of sheep immunoglobulin. | the "single-stage" mixed antiglobulin reaction (mar) was carried out with rabbit thymocytes. this test involved treating the cells with either sheep or goat anti-rabbit globulin sera, and subsequently reacting them with indicator erythrocytes coated with rabbit immunoglobulin (ig) so as to form rosettes. an unexpectedly high number (up to 38%) of thymocytes reacted, although the rosettes were weaker than those given by peripheral b lymphocytes. when blood and lymph node lymphocytes or thymus cel ... | 1975 | 46855 |
| dimeric and monomeric forms of hl-a antigens solubilized by detergent. | | 1975 | 46886 |
| presence of thymic antigen on rabbit basophils. | a goat heteroantiserum specific for a rabbit thymus lymphocyte antigen (rtla) reacted with rabbit basophils resulting in the release of histamine. this activity was equally absorbed out by thymocytes or basophils. absorption with either of these cell types also resulted in equal loss of thymocytotoxicity. no effect in either system occurred after absorption with either granulocytes or rabbit fibroblasts. these results could not be explained by the presence of immune complexes or aggregated globu ... | 1975 | 46897 |
| role of the eosinophil in the allergic reactions. i. edi-an eosinophil-derived inhibitor of histamine release. | an inhibitor of histamine release was found to be associated with the human eosinophilic leukocyte. this eosinophil-derived inhibitor (edi) was released from eosinophil-rich fractions upon sonication or interaction with immune reactants (specific allergens or anti-ige). edi was found to exert its inhibitory action at the target cell level by increasing the intracellular levels of cyclic-amp. preliminary electron microscopic studies show the presence of ige on the eosinophilic leukocyte and it is ... | 1975 | 46900 |
| isolation from individual a/j mice of anti-rho-azophenylarsonate antibodies bearing a cross-reactive idiotype. | immuization of a/j mice with a klh-p-azophenylarsonate conjugate induces the formation of antihapten antibodies, some of which share idiotypic specificity common to all recipients. the subpopulation carrying the idiotype generally comprises 20-70% of the total antibody content. large quantities of antihapten antibody (occasionally over 100 mg) were obtained from individual mice through the induction of an ascites fluid. this facilitated isolation of antibodies with the cross-reactive idiotype by ... | 1975 | 46907 |