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serological studies on sympatric barbary sheep and mule deer from palo duro canyon, texas.sera were collected from 12 barbary sheet (ammotragus lervia) and 11 mule deer (odocoileus hemionus) occupying sympatric ranges in palo duro canyon, texas. these were tested for leptospirosis, brucellosis, bovine virus diarrhea, anaplasmosis, vesicular stomatitis, bluetongue (bt), epizootic hemorrhagic disease (ehd), infectious bovine rhinotracheitis (ibr), and coccidioidomycosis. serologic reactors were found to ibr in 3 barbery sheep, bt in 6 barbary sheep and 6 mule deer and ehd in 3 barbary ...1979228088
conservation of nucleolus organizer regions during evolution in sheep, goat, cattle and aoudad.there are ten nucleolus organizer regions (nors) in domestic sheep (ovis aries l.). cattle (bos taurus l.), goat (capra hircus l.) and aoudad (ammotragus lervia blyth) and these are located terminally on chromosomes with homologous (g-banding patterns. the similarity in number of nucleolus organizer regions in these species may indicate that their ribosomal dna regions are infrequently involved in exchange events which could lead to different numbers of active nucleolus organizer regions. other ...1979476530
ectoparasites of sympatric barbary sheep and mule deer in the texas panhandle, usa. 1979541816
relationships between wild sheep and goats and the aoudad (caprini) studied by immunodiffusion. 1976819207
translocations of acrocentric chromosomes and their implications in the evolution of sheep (ovis).cytogenetic evidence suggests that the caprids (sheep and goats) evolved from a common ancestor with a 2n=60 karyotype. although goats (capra) retained the primitive 2n=60 karyotype, sheep (ovis) underwent a sequential reduction in the number of chromosomes by means of acrocentric translocation. the formation of the first metacentric autosome (m1) occurred in the aoudad (ammotragus) and urial (o. vignei), resulting in a 2n=58 karyotype. the g-bands are homologous, which implies both genotypes ar ...1976991654
rosette formation with sheep erythrocytes--a possible t-cell marker in the pig.a proportion of pig lymphocytes form rosettes with sheep erythrocytes. factors affecting their demonstration have been investigated, and a standard technique defined. rosette-forming lymphocytes lacked surface immunoglobulin detected by immunofluorescence and formation of rosettes was not inhibited by anti-immunoglobulin or anti-pla sera, but was by anti-thymus serum. of 18 species' erythrocytes tested only sheep, barbary sheep and mouflon erythrocytes formed rosettes in similar percentages. fet ...19751090543
progesterone and oestrogen concentrations in plasma of barbary sheep (aoudad, ammotragus lervia) compared with those of domestic sheep and goats during pregnancy.steroid hormone concentrations have been measured in the peripheral plasma of 3 barbary sheep over 3 breeding seasons. during pregnancy mean progesterone values rose initially and after a small decline between days 30 and 50, increased again and remained between 17 and 28 nmol/l until the last 2 days of pregnancy. oestradiol-17 beta reached a peak of about 300 pmol/l during mid-pregnancy, increasing to over 400 pmol/l in the last 5 days of pregnancy. oestrone sulphate began to increase in concen ...19902172532
alcelaphine herpesviruses 1 and 2 sds-page analysis of virion polypeptides, restriction endonuclease analysis of genomic dna and virus replication restriction in different cell types.herpesviruses have been isolated from white-tailed, white-bearded and blue wildebeest, as well as from jimela topi and cape hartebeest. these animals are members of the sub-family alcelaphinae of the family bovidae. viruses isolated from wildebeest cause malignant catarrhal fever (mcf) in susceptible ruminant species. alcelaphine herpesviruses (ahv) isolated from wildebeest replicate in both fetal aoudad sheep kidney (fak) cells and bovine embryonic lung (bel) cells. however, virus isolates from ...19892549921
l-antigen and active potassium transport in hk and lk red cells of barbary sheep (ammotragus lervia).1. the potassium concentration in red cells of 21 barbary sheep showed a bimodal distribution, with five animals of lk type (k+ conc. 30-45 mm) and 16 of hk type (k+ conc. 80-95 mm). 2. evidence is presented that both lp and ll antigens are present on lk barbary sheep red cells. 3. active k+ transport in lk barbary sheep red cells was stimulated 3-5 fold by sheep and goat anti-l. 4. active k+ transport in hk barbary sheep red cells was higher than in lk red cells. five out of six hk animals test ...19872887356
evidence of loss of a suggested genetic polymorphism in the blood potassium contents of zoo-living barbary sheep ammotragus lervia (pallas 1777).1. concentrations of potassium and iron were measured in blood samples of 15 zoo-living barbary sheep by atomic absorption spectrophotometry. 2. in analogy with the situation in ovis it is suggested that a blood potassium polymorphism is also found in ammotragus. 3. however, in the frankfurt line at least, one of the presumed alleles is likely to be lost due to inbreeding.19882894918
haemolytic effect of pasteurella haemolytica on blood from young mammals.on agar plates containing young lamb blood, pasteurella haemolytica produces a wide outer zone of partial haemolysis in addition to the narrow zone of complete clearing seen on adult sheep blood agar. to determine whether this phenomenon was limited to lamb blood, samples from young animals of 20 mammalian species were examined. two species--the barbary sheep (ammotragus lervia) and scimitar horned oryx (oryx tao)--possessed blood that gave this effect provided that the samples were taken from y ...19883194597
hemoglobin types in saanen goats and barbary sheep: genetic and comparative aspects.by the use of the immobiline technique at ph ranges 7.0-7.6 and 6.9-7.9, 16 different hemoglobin (hb) phenotypes were observed in 61 english saanen goats. they are explained in this breed by a genetic theory of five beta-globin genes (a4, a6, a8, e, and d) and two closely linked alpha-globin loci ('alpha and "alpha) of which the "alpha has a variant allele, provisionally called "alpha x. family data together with observed and expected hb frequencies were in agreement with the genetic theory. amo ...19883228449
variation among species in the endocrine control of mammary growth and function: the roles of prolactin, growth hormone, and placental lactogen.prolactin, growth hormone, and placental lactogen form a family of structurally related hormones, which may have evolved from a common ancestral peptide. prolactin and growth hormone are present in all mammals, but the biological activity associated with placental lactogen has been detected in only some groups. attempts to detect placental lactogen using bioassay and radioreceptor assay are reported and have been unsuccessful in an insectivore (the shrew), a bat, an edentate (the armadillo), a l ...19863519707
percentage of glycosylated hemoglobin and serum concentration of glucose in the blood of japanese macaques and in three exotic ruminant species.serum glucose concentrations and percentages of glycosylated hemoglobin (ghb) were determined in 16 japanese macaques and in 19 exotic ruminants (4 markhor, 5 mouflon, 10 aoudad). mean (+/- sd) serum glucose concentration and ghb percentage in the japanese macaques were 73 +/- 21.8 mg/dl and 4.7 +/- 0.82%, respectively. serum glucose concentration was considered within the normal range for this species. the mean ghb percentage was within the normal range for man, indicating that ghb may be a val ...19863752690
g-band patterns, chromosomal homologies, and evolutionary relationships among wild sheep, goats, and aoudads (mammalia, artiodactyla). 19744136259
serum biochemic and hematologic parameters of adult aoudas (ammotragus lervia) in captivity. 19704246020
hemoglobin types in barbary sheep (ammotragus lervia pallas, 1777); absence of a betac production in a homozygous beta c(na) animal during severe anemia. 19725039380
haemoglobin alpha chain duplication in barbary sheep, ammotragus lervia, pallas, 1777. 19705439729
production of hemoglobin c in the moufflon (ovis musimon pallas, 1811) and the barbary sheep (ammotragus lervia pallas, 1777) during experimental anemia: amino acid composition of tryptic peptides from the beta b and bet c chains. 19705496230
naturally occurring atherosclerosis in aoudads (ammotragus lervia (pallas). 19705527697
effects of tactile and electrical stimuli upon release of vasoactive intestinal polypeptide in the mammalian penis.plasma levels of vasoactive intestinal polypeptide (vip) in the corpora cavernosa penis and dorsal penile veins greatly exceeded those measured in the limb or caudal veins during anaesthesia in various mammals (bennett's wallaby, barbary sheep, cheetah, puma, sooty mangabey, pigtail macaque and chimpanzee). tactile stimulation of the penis immediately before or during collection of blood samples resulted in an increase. in the wallaby, vip levels (mean +/- s.e.m.) in blood samples collected from ...19846420493
elaeophorosis in barbary sheep and mule deer from the texas panhandle.adult elaeophora schneideri were recovered from the common carotid artery and its branches in 14 of 14 mule deer, odocoileus hemionus, and 3 of 9 barbary sheep or aoudads, ammotragus lervia, from palo duro canyon in the texas panhandle. gross cutaneous lesions attributable to elaeophorosis in the barbary sheep varied from small circumscribed scars up to 10 cm in diameter usually on the poll or orbital region to extensive proliferative irregular encrustations on the frontal, temporal and orbital ...19816454793
age related haematological changes in captive barbary sheep (ammotragus lervia).full blood counts were carried out on a number of clinically normal newborn, juvenile and adult barbary sheep. significant age related differences in the number and size of the red cells and in the absolute and relative numbers of neutrophils and lymphocytes were found. fibrinogen levels and platelet counts were lowest in newborn animals. these differences could complicate the interpretation of haematological findings on sick barbary sheep if not taken into account.19836622837
sources of progesterone during gestation in barbary sheep (ammotragus lervia).concentrations of progesterone in arterial and ovarian, uterine and jugular venous plasma were determined in four barbary sheep at various stages of pregnancy. the results, together with ovarian histology, show that the corpus luteum regresses before term in barbary sheep, as in most breeds of domestic ewes. uterine synthesis of progesterone was demonstrated in late pregnancy in two animals in which uterine venous levels of progesterone were increased two- to fourfold above arterial concentratio ...19836875435
further studies on caprine and ovine mycoplasmas related to mycoplasma mycoides subsp. mycoides.nine caprine and ovine mycoplasma strains, said to be indistinguishable serologically from mycoplasma mycoides subsp. mycoides (the causative organism of contagious bovine pleuropneumonia; cbpp) were examined in mice by (1) a mycoplasmaemia test, and (2) a cross-protection test. of the nine strains, two from goats belonged to a small colony (sc) type; four caprine and three ovine strains belonged to a large colony (lc) type.the two sc strains - like a single sc strain examined in an earlier stud ...19807005327
a cytogenetic study of a barbary sheep (ammotragus lervia) x domestic goat (capra hircus) hybrid.an account is given of stillborn male twins born to a female saanen goat (capra hircus) and a barbary ram (ammotragus lervia). the cytogenetics of the cultured hybrid cells are described and attention is drawn to the high proportion of cells which lacked one chromosome.19807193597
nucleolus organizer region location and 'ring' chromosomes in the bharal.silver-staining has been used to identify the nucleolus organizer regions (nors) in the bharal. these show homology with sheep, goat, cattle and aoudad. the association of the nors on both telomeres of chromosome 3 results in a 'ring' chromosome.19807371749
studies on hybridization between a barbary ram (ammotragus lervia) and domestic ewes (ovis aries) and nanny goats (capra hircus).six nanny goats and 9 ewes were inseminated with barbary semen and 4-5 days after insemination 16 hybrid embryos were recovered: 14 were transferred to ewes or nanny goats. survival of embryos was monitored by return to service after transfer, peripheral plasma progesterone values and by examination at laparotomy. none of the barbary ram x ewe embryos transferred to 4 ewes and 4 nanny goats or the barbary ram x nanny goat embryos transferred to ewes survived. of the 4 nanny goat recipients of ba ...19817452629
comparative aspects of biochemical polymorphism in the blood of caprinae species and their hybrids.biochemical variation at 14 blood loci was reviewed, and specific features compared experimentally in sheep ovis aries, mouflon ovis musimon, goat capra hircus, aoudad ammotragus iervia and in 2 stillborn aoudad x goat hybrids. variation at 3 loci was also studied in dall sheep ovis dalli, bighorn sheep ovis canadensis and rocky mountain goat oreamnos americanus. haemoglobin c production in an anaemic hb ab mouflon and in mouflon x sheep hybrids was examined. mouflon differ from domestic sheep i ...19807458003
sarcoptic mange in wild ruminants in zoological gardens in israel.sarcoptic mange (sarcoptes scabiei) occurred among wild ruminant species in five zoological gardens in israel, from 1984 to 1994. infestation of five ruminants by s. scabiei is reported for the first time: mountain gazelles (gazella gazella), nubian ibexes (capra ibex nubiana), a barbary sheep (ammotragus lervia), elands (taurotragus oryx), and an arabian oryx (oryx leucoryx). all animals in the herds were administered ivermectin orally at a dose of 200 micrograms/kg body weight daily for 3 cons ...19968627937
acute pleuropneumonia in barbary sheep (amnotragus lervia) associated with chromobacterium violaceum. 19968736506
immunohistochemical colocalization of serotonin, substance p and met-enkephalin-arg6-gly7-leu8 in the endocrine cells of the ruminant duodenum.the duodenum of various ruminants was examined by immunohistochemical staining for serotonin, substance p and metenkephalin-arg6-gly7-leu8 (menk8). serotonin- and substance p-immunoreactive endocrine cells were detected in samples from cow, calf, sheep, goat, and barbary sheep, menk8-immunoreactive endocrine cells were detected exclusively in samples from cow and calf. substance p and menk8 immunoreactivity was found in serotonin-immunoreactive endocrine cells of cattle. substance p- and menk8-i ...19989505449
basic hematological values in some wild ruminants in captivity.analyses of hematological parameters were carried out on eight axis deer (cervus axis), 12 fallow deer (cervus dama), 16 red deer (cervus elaphus hippelaphus), three sambar (cervus unicolor), nine père david deer (elaphurus davidianus), 20 european bison (bison bonasus), seven nilgai (boselaphus tragocamelus), eight mouflon (ovis musimon), four white-bearded gnu (connochaetes taurinus) and six barbary sheep (ammotragus lervia). the following parameters were determined: packed cell volume, hemogl ...199910629960
mitochondrial dna from myotragus balearicus, an extinct bovid from the balearic islands.dna was extracted from teeth and bones of myotragus balearicus, a bovid that evolved in isolation on the balearic islands (western mediterranean) from the end of the miocene, becoming extinct 4,000 years bp, after the arrival of humans in the islands. the numerous morphological apomorphies of myotragus, most strikingly its dwarfism, frontal eyes, and ever-growing incisors, obscure its phylogenetic relationships with extant bovids. therefore, the recovery of genetic information from myotragus is ...200010750053
plant genetic diversity in the canary islands: a conservation perspective.the canary islands are an atlantic volcanic archipelago with a rich flora of approximately 570 endemic species. the endemics represent approximately 40% of the native flora of the islands, and approximately 20% of the endemics are in the e (endangered) category of the international union for conservation of nature. a review of allozyme variation in 69 endemic species belonging to 18 genera and eight families is presented. the average species-level genetic diversity (h(t)) at allozyme loci is 0.1 ...200010898768
the use of buck and ram extenders and two packaging systems to cryopreserve aoudad (ammotragus lervia) spermatozoa.because the aoudad has been hunted to near extinction, cryopreservation of their semen would be useful for dna conservation and for the possible re-establishment of captive bred animals to their former ranges. this study was conducted to investigate the effectiveness of cryopreserving aoudad spermatozoa. semen samples from four post-pubertal animals were collected using electro-ejaculation. microscopic analysis was performed to assess the percentages of progressively and non-progressively motile ...200010990348
morphology of the dorsal lingual papillae in the barbary sheep, ammotragus lervia.the dorsal lingual surface of a barbary sheep (ammotragus lervia) was examined by scanning electron microscopy (sem). the tongue was about 20 cm in length. there were about 30 vallate papillae on both sides. filiform, conical, fungiform and vallate papillae were found. the filiform papillae were distributed over the entire dorsal surface of the tongue, excepted for the lingual torus where conical papillae were present. the fungiform papillae were present rounded bodies, and more densely distribu ...200010998934
laparoscopic intrauterine insemination in barbary sheep (ammotragus lervia). 200011098389
the species and chromosomal distribution of the centromeric alpha-satellite i sequence from sheep in the tribe caprini and other bovidae.the evolution of chromosomes in species in the family bovidae includes fusion and fission of chromosome arms (giving different numbers of acrocentric and metacentric chromosomes with a relatively conserved total number of arms) and evolution in both dna sequence and copy number of the pericentromeric alpha-satellite i repetitive dna sequence. here, a probe representing the sheep alpha-satellite i sequence was isolated and hybridized to genomic dna digests and metaphase chromosomes from various b ...200011173832
immunohistochemical evaluation of the muscularis mucosae in the ruminant forestomach.the muscularis mucosae and condensed fibrous layer of the ruminant forestomach were studied by immunohistochemistry using specific antibodies against alpha-smooth muscle actin (alphasma) and gamma-smooth muscle actin (gammasma). the specimens were collected from the rumen, reticulum and omasum of cattle, water buffalo, sheep, goat, barbary sheep, japanese serow, sika deer and mouse deer. the muscularis mucosae showed immunoreactivity for both alphasma and gammasma. on the other hand, the condens ...200312823104
erythrocyte survival studies in the mule deer, aoudad sheep, and springbok antelope, using glycine-2-c14. 195913812007
comparison between the g-banded karyotype of the aoudad (ammotragus lervia) and sheep (ovis aries).karyotypes of the aoudad and sheep were compared on the basis of g-banded chromosomes at the 450 band level. the common g-banded karyotype showed the homology of all aoudad chromosomes (2n=58) with sheep chromosomes (2n=54) or sheep chromosome arms. the results of cytogenetic investigations suggest that in this case karyotype evolution has led to reduction in chromosome number as a result of centric fusions. the formation of the first metacentric chromosome occurred in the aoudad. the homology o ...200114564064
population effects of sarcoptic mange in barbary sheep (ammotragus lervia) from sierra espuña regional park, spain.the nonindigenous barbary sheep population (ammotragus lervia) of the sierra espuna regional park (murcia, spain) suffered an outbreak of sarcoptic mange between 1991 and 1995, which contributed to a population decrease of 86%. this study presents the results of two population surveys conducted in 1994 and 1999 based on the fixed point and itineraries method (fpi) and the excrement count (ec) method, as well as data from demographic estimates and clinical observations conducted by the regional a ...200415465713
malignant catarrhal fever in a barbary sheep (ammotragus lervia). 200415518409
molecular evidence for the polyphyly of the genus hemitragus (mammalia, bovidae).the genus hemitragus includes three species of tahr distributed in distant geographical areas: the himalayan tahr, h. jemlahicus, occupies the southern flanks of the himalaya mountains; the nilgiri tahr, h. hylocrius, is endemic to southern india; and the arabian tahr, h. jayakari, is unique to the mountains of south-east arabia. all previous investigations were based on morphology, and these three species together have never been included in a molecular phylogenetic study. in this study, we con ...200515904863
prevalence of toxoplasma gondii antibodies in red deer (cervus elaphus) and other wild ruminants from spain.serum samples from 441 red deer (cervus elaphus) and 161 other wild ruminant species, collected between 1993 and 2005 from six regions of spain were tested for antibodies against toxoplasma gondii by the modified agglutination test (mat). antibodies to t. gondii (mat 1:25 or higher) were detected in 15.6% of red deer. statistically significant differences were observed among sampling sites with seroprevalence in red deer from catalonia (42.2%) being significantly higher compared with other spani ...200616359801
balantidiasis in the gastric lymph nodes of barbary sheep (ammotragus lervia): an incidental finding.a 4-year-old female barbary sheep (ammotragus lervia) was found dead in the gwangju uchi park zoo. the animal had previously exhibited weakness and lethargy, but no signs of diarrhea. the carcass was emaciated upon presentation. the main gross lesion was characterized by severe serous atrophy of the fat tissues of the coronary and left ventricular grooves, resulting in the transformation of the fat to a gelatinous material. the rumen was fully distended with food, while the abomasum evidenced mu ...200616645350
studies of haemoglobin types in barbary sheep (ammotragus lervia).1. two haemoglobin types, haemoglobins amm-c and amm-b, were observed in five barbary sheep (ammotragus lervia). one animal was homozygous for haemoglobin amm-c, a second was homozygous for haemoglobin amm-b, and three were heterozygous for both. 2. amino acid analyses of the globin from haemoglobin amm-b showed that this type was related to, but not identical with, haemoglobin b of the domestic sheep. 3. the beta-chain of haemoglobin amm-c was found to be composed of 141 amino acid residues. it ...196816742600
seroprevalence of neospora caninum in non-carnivorous wildlife from spain.serum samples from 1034 non-carnivorous wildlife from spain were tested for antibodies to neospora caninum by competitive screening enzyme linked immunosorbent assay (elisa) and confirmed by an indirect fluorescent antibody test (ifat). high agreement was observed between results in both techniques (kappa value higher than 0.9). prevalences of n. caninum antibodies positive by both techniques were 11.8% of 237 red deer (cervus elaphus), 7.7% of 13 barbary sheep (ammotragus lervia), 6.1% of 33 ro ...200716962706
barbary sheep (ammotragus lervia): the structure of the adult beta-globin gene and the functional properties of its hemoglobin.the adult beta-globin gene of ammotragus lervia (barbary sheep or aoudad) has been sequenced completely, including 185 base pairs of 5' flanking region and 168 base pairs 3' to the stop codon, and compared with that of other caprines such as goat (capra hircus), wild corsico-sardinian (ovis aries musimon) and cypriot (o. a. ophion) mouflons, and domestic sheep (ovis aries). the gene was identified as being located on a triplicated four-gene set cluster containing the hbba locus (a-haplotype) as ...200616962804
molecular evolution of coding and non-coding sequences of the growth hormone receptor (ghr) gene in the family bovidae.the ghr gene exon 1a and exon 4 with fragments of its flanking introns were sequenced in twelve bovidae species and the obtained sequences were aligned and analysed by the clustalw method. in coding exon 4 only three interspecies differences were found, one of which had an effect on the amino-acid sequence--leucine 152 proline. the average mutation frequency in non-coding exon 1a was 10.5 per 100 bp, and was 4.6-fold higher than that in coding exon 4 (2.3 per 100 bp). the mutation frequency in i ...200617044257
serologic responses of barbary sheep (ammotragus lervia), indian antelope (antilope cervicapra), wallaroos (macropus robustus), and chimpanzees (pan troglodytes) to an inactivated encephalomyocarditis virus vaccine.encephalomyocarditis virus (emcv) is a picornavirus with a worldwide distribution, capable of infecting a wide range of species. episodes of emcv-associated mortality have been reported in zoos and national parks around the world, including sporadic cases at taronga zoo, sydney. an inactivated emcv vaccine was evaluated by inoculating barbary sheep (ammotragus lervia), indian antelope (antilope cervicapra), eastern wallaroos (macropus robustus), and chimpanzees (pan troglodytes). a proportion of ...200517315459
chemical restraint of exotic animals in a disease regulatory program.the african red tick (rhipicephalus evertsi neuman) is widely distributed in africa but had never been identified in north america until september 1960 when it was found in a wild animal compound in florida. the red tick is a vector of cattle fever (piroplasmosis) and several other exotic diseases including the highly fatal east coast fever. the 130 acre compound contained more than 300 african and asian animals, including giraffes, camels, zebras, elands, nilghais, blackbuck, aoudads, ostriches ...196317649416
molecular characterization of cryptosporidium from animal sources in qinghai province of china.the presence of cryptosporidium oocysts in 20 zoo animals of the xining zoo, 16 farm yaks and 42 farm goats in qinghai province, china was investigated by an immunofluorescence test (ift). the species and/or genotypes were determined by nested polymerase chain reaction (pcr) and sequence analysis of a fragment of the small subunit (ssu) rrna gene. cryptosporidium oocysts were found in 16 zoo animals, 2 yaks, and 15 goats by ift. the ift positive samples were further investigated by pcr, and 16 o ...200717701218
complete nucleotide mtdna sequence of barbary sheep (ammotragus lervia).in this report we describe the complete sequence of the mtdna genome of ammotragus lervia (barbary sheep or aoudad) as obtained by pcr and sequencing with primer walking using flanking sequences. the molecule is 16,530 base pairs in length, resulting similar to those of goat and sheep. the genome organization matches to those of other mammalian mitochondrial genomes. the phylogenetic position of the ammotragus relative to twelve other mammalian species was assessed based on protein-coding sequen ...200817852329
a gene duplication affecting expression of the ovine asip gene is responsible for white and black sheep.agouti signaling protein (asip) functions to regulate pigmentation in mice, while its role in many other animals and in humans has not been fully determined. in this study, we identify a 190-kb tandem duplication encompassing the ovine asip and ahcy coding regions and the itch promoter region as the genetic cause of white coat color of dominant white/tan (a(wt)) agouti sheep. the duplication 5' breakpoint is located upstream of the asip coding sequence. ubiquitous expression of a second copy of ...200818493018
high prevalence of antibodies against chlamydiaceae and chlamydophila abortus in wild ungulates using two "in house" blocking-elisa tests.few data are available on the prevalence and relevance of chlamydiae in wild mammals, and even fewer studies have been conducted to determine the prevalence of chlamydophila abortus in wildlife hosts, most probably due to the absence of suitable species-specific serological assays for testing sera from wild animals. in light of this, we have developed two in-house blocking-elisa tests for detection of antibodies against chlamydiaceae and c. abortus in wild ungulates, and analyzed the relationshi ...200919010612
coinfection is an important factor in epidemiological studies: the first serosurvey of the aoudad (ammotragus lervia).despite being considered an invasive ungulate outside its native range (north africa), little information exists regarding the role of the aoudad (also called barbary sheep, ammotragus lervia) as a pathogen reservoir. furthermore, in most epidemiological surveys the potential role of coinfections (e.g. a first infection may make the host more immuno-competent or susceptible against a second pathogen) as a risk factor is often neglected. in this study we first performed a serological survey for s ...200919020912
investigation and diagnosis of nontuberculous mycobacteriosis in a captive herd of aoudad (ammotragus lervia).an epizootic of nontuberculous mycobacteriosis occurred in a captive herd of aoudad (ammotragus lervia) over a period of 18 mo. each of the affected animals was subject to a thorough postmortem examination that included histopathology, tissue concentration and acid-fast staining, aerobic and anaerobic bacterial culture, mycobacterial culture, and real-time polymerase chain reaction specific for mycobacterium tuberculosis dna. histopathologic lesions consistent with pulmonary mycobacteriosis, inc ...200919569478
pemphigus foliaceus in a barbary sheep (ammotragus lervia). 200919855115
spatial distribution and risk factors of brucellosis in iberian wild ungulates.the role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. the aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several iberian wild ungulates.201020205703
the sequence and phylogenesis of the ?-globin genes of barbary sheep (ammotragus lervia), goat (capra hircus), european mouflon (ovis aries musimon) and cyprus mouflon (ovis aries ophion).in order to investigate the polymorphism of ?-globin chain of hemoglobin amongst caprines, the linked (i)? and (ii)? globin genes of barbary sheep (ammotragus lervia), goat (capra hircus), european mouflon (ovis aries musimon), and cyprus mouflon (ovis aries ophion) were completely sequenced, including the 5? and 3? untranslated regions. european and cyprus mouflons, which do not show polymorphic ? globin chains, had almost identical ? globin genes, whereas barbary sheep exhibit two different ch ...200920403763
Fecal steroid evaluation to monitor reproductive status in wild ungulate females using enzyme immunoassay commercial kits.Analysis of reproductive hormones in fecal samples is necessary for the noninvasive monitoring of reproductive status in free-ranging species. The aim of the present study was to establish an easy noninvasive method to monitor reproductive status in wild ungulate females. Feces were collected daily, weekly, or three or four times a week directly from the soil for a period ranging from 1 to 9.8 mo. Fecal estradiol and progestagens were monitored in nine wild ungulate females (Barbary sheep, Ammot ...201122204046
morphology of the lingual papillae in the sitatunga.we examined the dorsal lingual surfaces of an adult sitatunga (tragelaphus spekei) by scanning electron microscopy. filiform, fungiform and vallate papillae were observed. the filiform papillae consisted of a larger main papilla and smaller secondary papillae. the filiform papilla contained connective tissue core consisting of several processes. the fungiform papillae were round in shape. the connective tissue core of the fungiform papilla was flower-bud shaped. lenticular papillae were limited ...201121882593
Y-chromosome phylogeny in the evolutionary net of chamois (genus Rupicapra).The chamois, distributed over most of the medium to high altitude mountain ranges of southern Eurasia, provides an excellent model for exploring the effects of historical and evolutionary events on diversification. Populations have been grouped into two species, Rupicapra pyrenaica from southwestern Europe and R. rupicapra from eastern Europe. The study of matrilineal mitochondrial DNA (mtDNA) and biparentally inherited microsatellites showed that the two species are paraphyletic and indicated a ...201121943106
blood groups and evolutionary relationships among domestic sheep (ovis aries), domestic goat (capra hircus), aoudad (ammotragus lervia) and european mouflon (ovis musimon). 022896160
diversity of the subspecies bifidobacterium animalis subsp. lactis.strains of bifidobacterium animalis subsp. lactis are well-known health-promoting probiotics used commercially. b. animalis subsp. lactis has been isolated from different sources, and little is known about animal isolates of this taxon. the aim of this study was to examine the genotypic and phenotypic diversity between b. animalis subsp. lactis strains different animal hosts including cameroon sheep, barbary sheep, okapi, mouflon, german shepard) and to compare to bb12, food isolates and the col ...201728108391
a serologic survey of viral infections in captive ungulates in turkish zoos.zoos and zoologic gardens make optimal environments for interspecies transmission of viral infections. there are seven zoos and several small zoologic collections in turkey. this study aimed to determine the current status of viral infections in captive ungulates living in these environments. blood samples were taken from 163 captive animals from two zoos. there were 39 cameroon sheep (ovis ammon f aries), 11 barbary sheep (ammotragus lervia), 57 pygmy goats (capra hircus), 9 angora goats (capra ...201122946369
first description of gastrointestinal nematodes of barbary sheep (ammotragus lervia): the case of camelostrongylus mentulatus as a paradigm of phylogenic and specific relationship between the parasite and its ancient host.the gastrointestinal helminth fauna of 24 barbary sheep or aoudad (ammotragus lervia sahariensis) maintained in the parque de rescate de la fauna sahariana (prfs, csic, almeria, spain) was analyzed. most animals (87.5 %) were parasitized, and multiple infections were highly present. the following species were identified: camelostrongylus mentulatus, teladorsagia circumcincta, marshallagia marshalli, ostertagia ostertagi, o. leptospicularis, o. lyrata, haemonchus contortus, teladorsagia trifurcat ...201323616093
distribution of mycobacterium avium ssp. paratuberculosis in a german zoological garden determined by is900 semi-nested and quantitative real-time pcr.little data concerning the distribution of mycobacterium avium ssp. paratuberculosis (map) in zoological gardens is available. the presence of map in captured wildlife might provide further information on non-ruminant hosts and expand the list of animals susceptible to map being potential sources of map transmission. therefore, a german zoological garden with recent history of clinical paratuberculosis in barbary sheep (ammotragus lervia) and an alpaca (lama pacos) was selected to estimate the d ...201323298700
occurrences of anti-toxoplasma gondii and anti-neospora caninum antibodies in barbary sheep at curitiba zoo, southern brazil.barbary sheep (ammotragus lervia) have the potential to act as hosts of important infectious diseases, particularly zoonoses. blood samples from 17 barbary sheep at the curitiba zoo were collected to evaluate occurrences of anti-toxoplasma gondii and anti-neospora caninum antibodies, tested using the indirect immunofluorescence antibody test (ifat). anti-t. gondii and anti-n. caninum antibodies were detected in 4/17 (23.5%) and 4/17 (23.5%) samples, respectively. the present study has shown that ...201425054509
concurrent spindle-cell thymoma and thymic cysts in a barbary sheep (ammotragus lervia): case report and review of the literature.an ~21-year-old female barbary sheep (ammotragus lervia) died spontaneously following a lengthy episode of difficulty in walking. an ~6 × 3 × 3 cm, unilocular cystic growth was found in the cranioventral thorax. the fibrotic cystic wall, lined by a single layer of flattened to cuboidal epithelial cells, was invaginated and partially encircled solid masses of fusiform neoplastic cells with multiple intratumoral cystic structures. the fusiform neoplastic cells were intensely positive for cytokerat ...201627698165
the eye of the barbary sheep or aoudad (ammotragus lervia): reference values for selected ophthalmic diagnostic tests, morphologic and biometric observations.the purpose of this study was to describe the normal ocular anatomy and establish reference values for ophthalmic tests in the barbary sheep or aoudad (ammotragus lervia). aoudad eyes are large and laterally positioned in the head with several specialized anatomic features attributed to evolutionary adaptations for grazing. normal values for commonly used ophthalmic tests were established, schirmer tear test (stt) - 27.22 ± 3.6 mm/min; predominant ocular surface bacterial microbiota - staphyloco ...201627419103
effect of shortening the prefreezing equilibration time with glycerol on the quality of chamois (rupicapra pyrenaica), ibex (capra pyrenaica), mouflon (ovis musimon) and aoudad (ammotragus lervia) ejaculates.the present study reports the effect of shortening the prefreezing equilibration time with glycerol on the quality of frozen-thawed ejaculated sperm from four mediterranean mountain ungulates: cantabrian chamois (rupicapra pyrenaica), iberian ibex (capra pyrenaica), mouflon (ovis musimon) and aoudad (ammotragus lervia). ejaculated sperm from these species were divided into two aliquots. one was diluted with either a tris-citric acid-glucose based medium (tcg-glycerol; for chamois and ibex sperm) ...201627346588
variation of meiotic recombination rates and mlh1 foci distribution in spermatocytes of cattle, sheep and goats.despite similar genome sizes, a great variability in recombination rates is observed in mammals. we used antibodies against sycp3, mlh1 and centromeres to compare crossover frequency, position along chromosome arms and the effect of crossover interference in spermatocytes of 4 species from the family bovidae (bos taurus, 2n = 60, tribe bovini; ovis aries, 2n = 54, capra hircus, 2n = 60 and ammotragus lervia, 2n = 58, tribe caprini). despite significant individual variability, our results also sh ...201526406935
cryopreservation of aoudad (ammotragus lervia sahariensis) sperm obtained by transrectal ultrasound-guided massage of the accessory sex glands and electroejaculation.this study examines (1) the effectiveness of transrectal, ultrasound-guided massage of the accessory sex glands (tumasg) combined with electroejaculation for obtaining aoudad (ammotragus lervia sahariensis) sperm samples for cryopreservation, and (2) the effectiveness of a tris-citric acid-glucose-based medium (tcg; usually used for freezing ibex sperm) and a tes-tris-glucose-based medium (ttg; typically used in the cryopreservation of mouflon sperm) as sperm extenders. after tumasg, just one to ...201323158213
characterization of the estrous cycle and reproductive traits of the aoudad (ammotragus lervia) in captivity.in this study the estrous cycle of the aoudad has been analyzed and characterized for the first time, using non-invasive methods for tracking reproductive cyclicity. the duration of the estrous cycle is 23 days (range 16-32 days), with a luteal phase of 17 days (range 12-27 days) and an interluteal phase of 6 days (range 3-14 days). the estrous cycle did not differ between females, but it was affected by the time of the year. intraindividual variation of the cycle was observed in one out of the ...201222365699
seroprevalence of neospora caninum and toxoplasma gondii in exotic ruminants and camelids in the czech republic.neospora caninum and toxoplasma gondii are the protozoan parasites with definitive hosts from order carnivora. due to vertical transmission, both parasites can cause abortions and neonatal mortality that lead to significant productive and economic losses in the domestic ruminants. the aim of this study was to describe n. caninum and t. gondii seroprevalence in the group of frequently farmed captive exotic ruminants (n = 184) including bovidae (barbary sheep, bezoar goat, common eland, american b ...201728497227
molecular screening for hemotropic mycoplasmas in captive barbary sheep (ammotragus lervia) in southern brazil.this study is part of an active surveillance program for monitoring animal health status in endangered species, and was conducted to screen captive barbary sheep (ammotragus lervia) for hemoplasma infection.201728919684
first report of oestrosis in aoudad from southeastern spain.in spring 2016, we analyzed the skull of 44 aoudads (ammotragus lervia) from sierra espuña regional park (se spain) for the presence of oestrid larvae. oestrus ovis larvae were found in 27.3% of sampled hosts, with a mean intensity of 1.5 ± 6.1 larvae/per parasitized host (range 1-21). to our knowledge, this is the first report of oestrosis affecting this host species.201728508162
molecular identification of parasitic nematodes (nematoda: strongylida) in feces of wild ruminants from tunisia.in tunisia and other north african countries, there is a lack of knowledge about parasite biodiversity within threatened wild ruminants and there are not any studies on their gastrointestinal nematodes. thus the aim of this study was to identify gastrointestinal fauna in the faecal samples of tunisian wild ruminants. a total of 262 faecal samples were collected from domestic sheep and goat, and wild ruminants (addax, barbary sheep, barbary red deer, dorcas gazelle, slender-horned gazelle and sci ...201729113593
diagnosis of pregnancy in barbary sheep (ammotragus lervia) using a bovine assay for pregnancy-associated glycoproteins.pregnancy diagnosis is an important part in reproduction management of wild ruminants involved in free-ranging and captive programs. pregnancy-associated glycoproteins (pags) are placenta-expressed proteins released into maternal blood circulation. tests with high specificity have been developed and validated in domestic species and have been used in some wild ungulate species. one hundred and seventeen serum samples collected from 72 mature female barbary sheep ( ammotragus lervia ) were tested ...201728749285
blood meal identification in the cryptic species anopheles gambiae and anopheles coluzzii using maldi-tof ms.matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) has recently emerged in entomology as a technique to identify arthropods and their blood meal source. in this study, female anopheles gambiae were fed on five host blood sources: ocelot (leopardus pardalis), binturong (arctictis binturong), springbok (antidorcas marsupialis), jaguar (panthera onca) and hamadryas baboon (papio hamadryas), while anopheles coluzzii were fed on three hosts: dromedary (camelus ...201830052501
multiple innate antibacterial immune defense elements are correlated in diverse ungulate species.in this study, we aimed to evaluate to what extent different assays of innate immunity reveal similar patterns of variation across ungulate species. we compared several measures of innate antibacterial immune function across seven different ungulate species using blood samples obtained from captive animals maintained in a zoological park. we measured mrna expression of two receptors involved in innate pathogen detection, toll-like receptors 2 and 5 (tlr2 and 5), the bactericidal capacity of plas ...201931774834
curious creatures: a multi-taxa investigation of responses to novelty in a zoo environment.the personality trait of curiosity has been shown to increase welfare in humans. if this positive welfare effect is also true for non-humans, animals with high levels of curiosity may be able to cope better with stressful situations than their conspecifics. before discoveries can be made regarding the effect of curiosity on an animal's ability to cope in their environment, a way of measuring curiosity across species in different environments must be created to standardise testing. to determine t ...201829568703
distribution of pestivirus exposure in wild ruminants in spain.a large-scale study was carried out to determine the prevalence of antibodies against pestivirus species in wild ruminants and describe their spatial variation in mainland spain. serum samples of 1,874 wild ruminants from different regions of this country were collected between the years 2000 and 2017. a total of 6.6% (123/1,874) animals showed antibodies against pestivirus by both blocking elisa (belisa) and virus neutralization tests (vnt). the prevalence of antibodies against pestiviruses was ...202032920992
serosurveillance of schmallenberg virus in wild ruminants in spain.schmallenberg disease (sbd) is an emerging vector-borne disease that affects domestic and wild ruminants. a long-term serosurvey was conducted to assess exposure to schmallenberg virus (sbv) in all the wild ruminant species present in mainland spain. between 2010 and 2016, sera from 1,216 animals were tested for antibodies against sbv using a commercial blocking elisa. the overall prevalence of antibodies was 27.1% (95%ci: 24.7-29.7). statistically significant differences among species were obse ...202032530115
aestipascuomycesdupliciliberans gen. nov, sp. nov., the first cultured representative of the uncultured sk4 clade from aoudad sheep and alpaca.we report on the isolation of the previously-uncultured neocallimastigomycota sk4 lineage, by two independent research groups, from a wild aoudad sheep rumen sample (texas, usa) and an alpaca fecal sample (baden-württemberg, germany). isolates from both locations showed near-identical morphological and microscopic features, forming medium-sized (2-5 mm) white filamentous colonies with a white center of sporangia, on agar roll tubes and a heavy biofilm in liquid media. microscopic analysis reveal ...202033167420
deep mitochondrial dna phylogeographic divergence in the threatened aoudad ammotragus lervia (bovidae, caprini).the aoudad or barbary sheep (ammotragus lervia) is a threatened ungulate emblematic of north africa, whose population structure and subspecific taxonomy have not been examined genetically. this knowledge is essential and urgently needed to inform ongoing conservation and management efforts. we analysed the mitochondrial cytochrome b gene and four nuclear genes (casein kappa, spectrin beta nonerythrocytic 1, thyroglobulin, thyrotropin subunit beta) for the first phylogeographic survey of the aoud ...202032109559
barbary sheep tissues as bioindicators of radionuclide and stabile element contamination in croatia: exposure assessment for consumers.muscle, liver and kidney of 21 barbary sheep (ammotragus lervia) from mosor mountain, croatia, were sampled to quantify the activity of caesium and potassium radionuclides and five toxic and ten essential stabile elements in order to establish reference values for this species and to evaluate the potential of barbary sheep tissues to reflect environmental pollution. we also assessed seasonal diet (botanical composition and dry matter content) of barbary sheep based on analyses of a rumen content ...201930877528
feramyces austinii, gen. nov., sp. nov., an anaerobic gut fungus from rumen and fecal samples of wild barbary sheep and fallow deer.anaerobic gut fungi are common inhabitants of the alimentary tracts of herbivorous animals. nine different neocallimastigomycota genera have been described so far. however, culture-independent diversity surveys suggest the presence of numerous, yet-uncultured neocallimastigomycota genera. here, we report on the isolation and characterization of the first cultured representatives of neocallimastigomycota clade al6, originally identified in culture-independent surveys of fecal samples from captive ...201929969376
monitoring of bluetongue virus in zoo animals in spain, 2007-2019.bluetongue (bt) is an emerging and re-emerging communicable vector-borne disease of animal health concern. a serosurvey was performed to assess exposure to bt virus (btv) in zoo animals in spain and to determine the dynamics of seropositivity in longitudinally sampled individuals during the study period. serum samples were collected from 241 zoo animals belonging to 71 different species in five urban zoos (a-e) in spain between 2007 and 2019. twenty-four of these animals were longitudinally surv ...202133963677
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