| evidence of a gastrin-like substance in rhinobatus productus. | | 1975 | 206 |
| mitochondrial traits of muscle from stress-susceptible pigs. | | 1975 | 352 |
| use of 5-deazafad to study hydrogen transfer in the d-amino acid oxidase reaction. | the apoprotein of hog kidney d-amino acid oxidase was reconstituted with 5-deazaflavin adenine dinucleotide (5-deazafad) to yield a protein which contains 1.5 mol of 5-deazafad/mol of enzyme. the deazafad-containing enzyme forms complexes with benzoate, 2-amino benzoate, and 4-aminobenzoate which are both qualitatively and quantitatively similar to those observed with native enzyme. the complex with 2-aminobenzoate exhibits a new long wavelength absorption band characteristic of a flavin charge- ... | 1975 | 390 |
| the effects of drinking on offspring: an historical survey of the american and british literature. | current research on the effects on offspring of drinking during pregnancy has revived interest in an extremely old topic. observations made during england's gin epidemic (1720-1750) were followed by warnings of 19th-century medical writers that parental drinking could damage the fetus. many concurring studies were reported in the medical literature from 1865 to 1920. research interest declined during prohibition, and authorities later discounted the previous work. recently a relationship between ... | 1975 | 545 |
| treatment of ischemic wounds in the elderly by covering with pigskin heterografts. | | 1975 | 565 |
| further studies on the ganglionic and smooth muscle stimulating effect of potassium on the peristaltic reflex of the guinea-pig isolated ileum. | | 1975 | 639 |
| the relation between myosin adenosinetriphosphatase activity and inactivation of myosin under alkaline conditions of heart muscles in mammals of different size. | atpase activity of myosin in the heart muscle of the mouse, rat, guinea-pig, rabbit and pig was studied at neutral ph and under mild alkaline conditions. at neutral ph the atpase activity of myosin is inversely related to body size of the animal species. the decrease of atpase activity of myosin after alkaline preincubation depends on the degree of atpase activity of intact myosin, i.e. myosin from the heart of the mouse exhibits high atpase activity ae same relationship was found, when comparin ... | 1975 | 651 |
| proceedings: comparison of the effects of selective alpha and beta-receptor agonists on intracellular cyclic amp levels and glycogen phosphorylase activity in guinea-pig liver. | | 1975 | 118 |
| myosin from arterial smooth muscle: isolation following actin depolymerization. | the contractile proteins from arterial smooth muscle are highly soluble, and can be extracted at i = 0.05. however, they can be precipitated by a prolonged dialysis at ph 6 to give an actomyosin with a high, although variable, actin:myosin ratio. the sedimentation behavior of this actomyosin at high ionic strength was examined as a function of ph, protein concentration and composition by preparative ultracentrifugation. comparisons with synthetic skeletal muscle actomyosins of similar compositio ... | 1975 | 85 |
| experimental ulcer. | | 1975 | 855 |
| effect of amphetamine-type psychostimulants on brain metabolism. | | 1975 | 902 |
| survival of salmonellae during pepperoni manufacture. | survival of salmonellae in artificially contaminated beef-pork mixtures (approximately 10(4) salmonellae/g) was studied in pepperoni prepared by either a natural flora or lactic starter culture fermentation or in nonfermented sausages. the pepperoni did not become salmonellae free during the usual commercial 15 to 30-day drying period. salmonella dublin was present in all products, fermented or unfermented, after 42 to 43 days of drying. at a lower level of contamination, 10(3)/g, s. dublin coul ... | 1975 | 951 |
| ampholyte displacement chromatography - a new technique for the separation of proteins illustrated by the resolution of beta-n-acetyl-d-hexosaminidase isoenzymes unresolvable by isoelectric focusing or conventional ion-exchange chromatography. | | 1975 | 1013 |
| on the isolation of a prolactin inhibiting factor (hormone). | | 1975 | 1015 |
| modification of human erythrocyte ghosts with transglutaminase. | | 1975 | 1028 |
| proposed mechanisms of stimulation and inhibition of guanylate cyclase with reference to the actions of chlorpromazine, phosphoipases and triton x-100. | | 1975 | 1032 |
| lysosomal hydrolases of the epidermis. 3. peptide hydrolases. | four distinct peptide hydrolases (ec 3-4) have been characterized in guinea-pig epidermis; these are cathepsin b1, cathepsin c, cathepsin d and arylamidase. their properties are consistent with those of lysosomal enzymes. cathepsin e was not detected. | 1975 | 1081 |
| the effect of sulphydryl compounds and cross linking agents on the viscous and viscoelastic properties of mucus. | | 1975 | 1125 |
| diphenylhydantoin-beta-adrenolytic combinations and digitalis poisoning. | | 1975 | 1173 |
| beta-adrenergic receptors in vas deferens of guineapigs. | | 1975 | 1343 |
| the mechanism of hydrolysis of beta-glycerophosphate by kidney alkaline phosphatase. | 1. to identify the functional groups that are involved in the conversion of beta-glycerophosphate by alkaline phosphatase (ec 3.1.3.1) from pig kidney, the kinetics of alkaline phosphatase were investigated in the ph range 6.6-10.3 at substrate concentrations of 3 mum-30 mm. from the plots of log vh+ against ph and log vh+/kh+m against ph one functional group with pk = 7.0 and two functional groups with pk = 9.1 were identified. these groups are involved in substrate binding. another group with ... | 1975 | 995 |
| the stimulation by synaptic transmitters of the incorporation of oleate into the phospholipid of synaptic membranes. | noradrenaline stimulated the incorporation of oleate into choline glycerophospholipids of guinea-pig brain synaptic membranes incubated in sodium phosphate buffer. in the presence of 1 mm-naf, noradrenaline stimulated the incorporation of oleate into the choline glycerophospholipids, phosphatidylinositol, ethanolamine glycerophospholipids, phosphatidylserine and phosphatidic acid of synaptic membranes incubated in 10 mm-tris-hcl buffer. in tris-chl containing 1 mm-naf, stimulation of incorporati ... | 1975 | 993 |
| ascorbic acid and biological systems. ascorbic acid and electron transport. | | 1975 | 941 |
| on the interactions between pancreatic lipase and colipase and the substrate, and the importance of bile salts. | the interactions between pancreatic lipase and colipase and the substrate and the effect of bile salts on these interactions have been investigated by the use of kinetic experiments and studies on the semiquantitative phase distribution of lipase and colipase activities. the results suggest that lipase binds to hydrophobic interfaces with partial irreversible inactivation. bile salts in the range of micellar concentrations and above a ph of about 6.5 displace lipase from this binding, resulting ... | 1975 | 446 |
| localization of nadh oxidase on the surface of human polymorphonuclear leukocytes by a new cytochemical method. | the ultrastructural localization of nadh oxidase, a possible enzyme in the increased oxidative activity of polymorphonuclear leukocytes (pmn) during phagocytosis, was studied. a new cytochemical technique for the localization of h2o2, a product of nadh oxidase activity, was developed. cerous ions, in the presence of peroxide, form an electron-dense precipitate. resting and phagocytically stimulated pmn were exposed to cerous ions at ph 7.5 to demonstrate sites of nadh-dependent, cyanide-insensit ... | 1975 | 407 |
| affinity labeling of d-amino acid oxidase with an acetylenic substrate. | the acetylenic substrate, d-2-amino-4-pentynoic acid (d-propargylglycine), was oxidatively deaminated by hog kidney d-amino acid oxidaseec 1.4.3.3, with accompanying inactivation of the enzyme. the flavin which was extracted by hot methanol from the inactivated enzyme was identical with authentic fad by thin-layer chromatography and circular dichroism. the excitation spectrum of emission at 520 nm of the released flavin was very similar to the absorption spectrum of oxidized fad. the released fl ... | 1975 | 379 |
| the pharmacology of (+/-)-1-(4-cyclohexylureido-phenoxy)-2-hydroxy-3-tert-butylaminopropane (talinolol, cordanum, 02-115). | | 1975 | 688 |
| structure-activity relationship of phenoxyalkanolamine derivatives with selective beta 1-receptor blocking action. | | 1975 | 687 |
| studies on the action of talinolol (cordanum, 02-115) on the coronary blood circulation. | | 1975 | 689 |
| liver microsomal beta-glucuronidase and udp-glucuronyltransferase. | both udp-glucuronyltransferase (gt) and beta-glucuronidase (betag) were assayed in untreated liver microsomes. optimum assay conditions were established with rat liver microsomes using p-nitrophenol (pnp) and its glucuronide (pnpga) at the ph optima of gt (7.5) and betag (4.5). the activities of the two enzymes were compared using microsomes from rats, mice, pigs, cattle and horses, with pnp, pnpga, and phenolphthalein as substrate, in the presence of various cofactors and inhibitors at ph 7.5 a ... | 1975 | 230 |
| some characteristics of two azoreductase systems in rat liver. relevance to the activity of 2-4'-di(2"-bromopropyl)-aminophenylazobenzoic acid (cb10-252), a compound possessing latent cytotoxic activity. | the system involved in the reduction of 2-4'-di(2''-bromopropyl) aminophenylazolbenzoic acid (cb10-252), an agent designed for treating primary liver cell cancer, has been demonstrated to be localised mainly in the 108 000 x g supernatant fraction of rat liver homogenate. it is also present in other organs particularly in the spleen. dab-azoreductase as shown previously is present almost entirely in the microsomal fraction and is found in high concentration only in liver. the ph maximum for cb10 ... | 1975 | 149 |
| precipitation of phase i antigen of coxiella burnetii by sodium sulfite. | | 1975 | 2000 |
| the involvement of lysophosphoglycerides in neurotransmitter release; the composition and turnover of phospholipids of synaptic vesicles of guinea-pig cerebral cortex and torpedo electric organ and the effect of stimulation. | (1) crude synaptosomal fractions (p2) derived from guinea-pig cerebral cortex were incubated in the presence of 50 mm kcl in a krebs-glucose medium. torpedo marmorata electric organs were stimulated electrically in vivo at 5 pulses/sec for 30 min by electrodes placed on the electric lobe. synaptic vesicles were isolated from each source and the phospholipid compositions analysed and compared with vesicles from unstimulated controls. (2) lysophosphatidylcholine was the only lysophosphoglyceride ... | 1975 | 129 |
| radioactive labeling and location of specific thiol groups in myosin from fast, slow and cardiac muscles. | 1. based on incorporation of radioactively labeled n-ethylmaleimide, the readily reactive thiol groups of isolated myosin (ec 3.6.1.3) from fast, slow and cardiac muscles could be classified into 3 types. all 3 myosins contain 2 thiol-1, 2 thiol-2 and a variable number of thiol-3 groups per molecule. both thiol-1 and thiol-2 groups which are essential for functioning of the k+-stimulated atpase, are located in the heavy chains in all 3 myosin types. 2. the variation in the incorporation pattern ... | 1975 | 73 |
| immunological polyfunctionality of proteins and its meaning for the analysis of an allergen-active bacterial substrate. | it was shown with the aid of immunosorption of an allergen-active substrate of e. coli 020: k84 (no. 2-rii) that protein substances taking part in the phenomenon of cell hypersensitivity were active in the humoral immunity reactions. the allergenic and immunochemical activity served as functions of the same molecules of bacterial proteins, this substantiating the use of immunochemical analysis for the study of an allergen-active bacterial substrate. by protein denaturing it is possible to obtain ... | 1975 | 1948 |
| calcium-stimulated adenosine triphosphatase in the microsomal fraction of tooth germ from porcine fetus. | the characterization and localization of a ca(2+)-atpase (atp phosphohydrolase, ec 3.6.1.3) in the tooth germ of the porcine fetus are reported. this enzyme, a microsome fraction, is preferentially activated by ca(2+). in the presence of 0.5 mm atp, maximal enzyme activity is obtained at 0.5--1.0 mm cacl2. the maximal rate of atp hydrolysis is approx. 20 mumol per h per mg of protein as the enzyme preparation is used here. at optimal ca(2+) concentration, the mg(2+) has an inhibitory effect. the ... | 1975 | 71 |
| monoanion inhibition and 35cl nuclear magnetic resonance studies of renal dipeptidase. | kinetic analyses of monoanion inhibition and 15cl nuclear magnetic resonance at 5.88 mhz were employed to study monoanion interactions with the zinc metalloenzyme, renal dipeptidase. the enzyme-catalyzed hydrolysis of glycyldehydrophenylalanine exhibited competitive inhibition when the reaction rate was determined in the presence of the monovalent anions fluoride, chloride, bromide, iodide, azide, nitrate, or thiocyanate or upon the addition of the divalent anion, sulfate. competitive inhibition ... | 1975 | 48 |
| lysosomal hydrolases of the epidermis. 2. ester hydrolases. | five distinct ester hydrolases (ec 3-1) have been characterized in guinea-pig epidermis. these are carboxylic esterase, acid phosphatase, pyrophosphatase, and arylsulphatase a and b. their properties are consistent with those of lysosomal enzymes. | 1975 | 31 |
| lysosomal hydrolases of the epidermis. i. glycosidases. | seven distinct glycosidases (ec 3.2) have been characterized in guinea-pig epidermis. their properties indicate them to be of lysosomal origin. the 'profile' of the epidermal glycosidases is significantly different from that reported for whole skin, the activities of beta-galactosidase and beta-acetylglucosaminidase being very high and those of the remaining enzymes relatively low in epidermis. | 1975 | 30 |
| avoidance of strongly chaotropic eluents for immunoaffinity chromatography by chemical modification of immobilized ligand. | the need for chaotropic eluents in immunoaffinity chromatography is a consequence of the high affinities of antibodies towards their antigens. this affinity is decreased and elution of antiglucagon antibodies from a column of immobilized glucagon can be achieved under mild conditions when the steric complementarity to the antibody binding site is perturbed by selective chemical modification of the hormone. the effects of reaction with 2-hydroxy-5-nitrobenzyl bromide, tetranitromethane and hydrog ... | 1976 | 2298 |
| partial purification and characterization of post-proline cleaving enzyme: enzymatic inactivation of neurohypophyseal hormones by kidney preparations of various species. | the inactivation of the neurohypophyseal hormones arginine vasopressin and oxytocin, both 14c-labelled in the c-terminal glycine residue, by enzymes present in kidney homogenates of various species has been investigated, and some of the enzymes responsible have been partially purified and characterized. the leu-gly peptide bond of oxytocin is generally most effectively cleaved by kidney homogenates, although with certain species enzymic activity hydrolyzing the pro-leu bond is significant. degra ... | 1976 | 2300 |
| pharmacological properties of new neuroleptic compounds. | rmi 61 140, rmi 61 144 and rmi 61 280 are newly synthetized n-8-r-dibenzo(b,f)oxepin-10-yl-n'-methyl-piperazine-maleates which show interesting psychopharmacologic effects. this work contains the results of a study performed with these three compounds, in order to demonstrate their neuropsycholeptic activity in comparison with chloropromazine (cpz) and chlordiazepoxide (cpd). the inhibition of motility observed in mice shows that the compounds reduce the normal spontaneous motility as well as th ... | 1975 | 25 |
| identification of adenylate cyclase-coupled beta-adrenergic receptors with radiolabeled beta-adrenergic antagonists. | | 1975 | 11 |
| classification and biological distribution of histamine receptor sub-types. | the distribution and classification of histamine receptors in mammalian and avian tissues have been summarized in tables 1-4. it is evident that histamine receptors are present on a number of morphologically distinct cell types and the proportion of cells bearing h1- and h2-receptors varies not only with the species but also with the cell source. the pharmacological receptors mediating mepyramine-sensitive histamine responses have been defined as h1-receptors. receptors mediating mepyramine-resi ... | 1975 | 1979 |
| the mechanism of action and treatment of palytoxin poisoning. | | 1975 | 1871 |
| positive inotropic effect of insulin on rabbit auricle in vitro. | water soluble pig insulin (4 x 10(-8) to 4 x 10(-7) g/ml) produced a marked and long-lasting increase in the contractile force of the rabbit auricle in vitro. once the maximum effect for a given insulin concentration had been reached, addition of more insulin did not produce any further increase in inotropic effect. insulin was without effect in reserpinized animals. inhibition of cardiac beta-receptors by propranolol suppressed the positive inotropic effect of insulin. these findings support th ... | 1975 | 2125 |
| investigations of the excretion of gamma-glutamyl-transpeptidase into the urine. | the excretion of the enzyme gamma-glutamyl-transpeptidase and its isoenzymes into the urine was investigated in patients with renal diseases and compared with the excretion of the enzymes leucine-aminopeptidase and lactate-dehydrogenase. in animal experiments an increased excretion of these enzymes was found after autotransplantation. increased excretion of gamma-glutamyl-transpeptidase was also found in patients with glomerulonephritis and in the polyuric phase of acute tubular necrosis, but no ... | 1975 | 1355 |
| glutathione reductase from human erythrocytes. molecular weight, subunit composition and aggregation properties. | glutathione reductase from human erythrocytes exists predominatly as an entity of 100 000 molecular weight under various conditions of ph and ionic strength. the s20,w of 5.5 s and d20w of 50 mum2/s correlate with the molecular weight determined by sedimentation equilibrium. the homogeneity of this species is primarily dependent on the presence of thiols and secondarily on high concentrations of salt. the amino-acid composition of the enzyme shows similarities both with glutathione reductases fr ... | 1975 | 1274 |
| time-dependent inhibition of diamine oxidase by carbonyl-group reagents and urea. | 1. the behaviour of several carbonyl group reagents and urea as time-dependent inhibitors of both pig kidney and human placental diamine oxidase is described. 2. plots of log (vt/vo) against time were not linear with these reagents as the usual theories predict. 3. this was particularly the case with aminoguanidine and phenylhydrazine and a thorough study of the effects of these compounds on the human placental diamine oxidase is described. 4. by applying a new theory for time-dependent inhibiti ... | 1975 | 1269 |
| amphetamine tachyphylaxis in the pithed guinea-pig. | a single dose of (+)-amphetamine (8 mg kg-1, i.p.) administered 4 h before experimentation, reduced the pressor and positive chronotropic effects elicited by this drug (0-6 mg kg-1, i.v.) and augmented the rate of the development of tachyphylaxis to these responses in the pithed guinea-pig preparation. amphetamine pretreatment reduced the pressor and positive chronotropic effects of phenylephrine (0-1 mg kg-1, i.v.) and the positive chronotropic effects of angiotensin (30 mug kg-1, i.v.). the ra ... | 1975 | 1490 |
| ganglion blocking activity of a few quaternised quinoline derivatives. | | 1975 | 2555 |
| total and regional cerebral blood flow during moderate and severe exercise in miniature swine. | to determine the influence of exercise on cerebral blood flow, we ran 14 swine at 3-6 mph and at 0-10% grades on a treadmill for 30 min at moderate and severe levels of exercise. measuring heart rate, cardiac output, and aortic pressure via implanted probes, we injected 15-mum radiolabeled microspheres via the left atrium before and during exercise. we measured their distribution by gamma spectrometry, determining total cerebral blood flow, regional blood flow, and ratio of flow to gray and whit ... | 1976 | 2577 |
| porcine chymotrypsin a-pi, a more acidic chymotrypsin. | a kinetic study of procine chymotrypsin a-pi revealed two characteristic properties of this type of chymotrypsin: 1. porcine chymotrypsin a-pi, like bovine chymotrypsin b-pi does not bind proflavin, which is a competitive inhibitor of bovine trypsin and chymotrypsin a-alpha. 2. the ph profiles of the steady-state parameters show the two usual important pk's. the basic one, pk2 = 9.6, affects both km and kcat/km and probably controls the binding conformation of chymotrypsin. the acidic one, pk1 = ... | 1975 | 1143 |
| phospholipase a2 isoenzyme from porcine pancreas. purification and some properties. | porcine pancreas synthesizes a prephospholipase a2 which occurs in a 5 : 95 ratio compared with the more abundant zymogen of the same enzyme (phosphatide-acylhydrolase; ec 3.1.1.4). these two prephospholipases could be well separated by cm-cellulose chromatography. both the active and the zymogen form of the isoenzyme were isolated and purified. the activation peptides of both prephospholipases appeared to be identical, while the active enzymes showed a few interesting differences. the most stri ... | 1975 | 1091 |
| adenosine triphosphate: nicotinamide mononucleotide adenylyltransferase of pig liver. purification and properties. | adenosine triphosphate : nicotinamide mononucleotide adenylyltransferase (ec 2.7.7.1) has been purifiec approximately 3500-fold from an extract of pig liver nuclei to a specific activity of 40 mumol of nad+ per min per mg protein. the enzyme was found to have a molecular weight of 203 000, a frictional ratio of 1.6 and an isoelectric point of approximately 5. michaelis constants for atp and nmn were 0.11 mm and 0.12 mm, respectively. | 1975 | 1090 |
| boar seminal zinc-precipitable protein and the haemagglutinin. | about 30% of boar seminal plasma nitrogen is maximally precipitated at room temperature by 6 to 10 mm zinc in citrate solution at ph 8. a rise in the total nitrogen precipitated by 1 to 6 mm zinc is accompanied by a fall in the haemagglutinin titre of the supernatant fluid. at 6 mm zinc addition, 95% of the haemagglutinin is precipitated, but much of this is recoverable by re-solution of the zinc precipitate. protein profile studies by gel-filtration chromatography of the zinc precipitate soluti ... | 1975 | 1526 |
| non-specific alkaline phosphomonoesterases of eight species of digenetic trematodes. | alkaline phosphatases from different trematodes occupying the same habitat have identical ph otima but different levels of enzyme activities. isoparorchis hypselobagri, from the fish wallago attu, shows four to six times more enzyme activity than fasciolopsis buski, gastrodiscoides hominis and echinostoma malayanum, from the pig sus scrofa, and fasciola gigantica, gigantocotyle explanatum, cotylophoron cotylophorum and gastrothylax crumenifer, from the buffalo bubalus bubalis. at least two peaks ... | 1975 | 1442 |
| enzymic deacetylation of carcinogenic arylacetamides by tissue microsomes of the dog and other species. | the relative ability of arylacetamide deacetylase enzyme systems of dog liver to carry out the deacetylation of the carcinogens, 4-acetylaminobiphenyl, 2-acetylaminofluorene, and 2-acetylaminaphthalene, was examined. the arylacetamides were incubated with unfortified dog liver microsomes, and enzyme activity (nmol arylamine/mg protein/hr) was estimated by colorimetric quantitation of the resulting arylamines. the dog liver enzyme system displayed characteristics similar to those described for th ... | 1976 | 1550 |
| plasma glucose in miniature swine infused with ethanol and fructose. | hypoglycemia resulting from intragastric infusion of ethanol in miniature swine was counteracted by simultaneous infusion of fructose. the magnitude and time of peak plasma ethanol concentrations were functions of the quantity of ethanol or ethanol and fructose. | 1976 | 2808 |
| cholesterol and fatty acid synthesis in swine. | in incubation studies with swine tissue slices, acetate-1-14c or glucose-u-14c as substrates were incorporated more readily into fatty acids and cholesterol in adipose tissue than other tissues tested. cholesterol and fatty acid synthesizing acitivity was substantial in the small intestine. when acetate was available, liver, small intestine, and adipose tissue were important sites for cholesterol synthesis. heart and aortic tissue had marginal levels of cholesterol synthesizing ability. lipogene ... | 1976 | 2832 |
| effects of colipase and taurodeoxycholate on the catalytic and physical properties of pancreatic lipase b at an oil water interface. | a monolayer reaction system employing tripropionin and siliconized glass beads was used to study the effects of taurodeoxycholate and colipase on the catalytic activity, interfacial stability, and interfacial affinity of porcine pancreatic lipase b (ec 3.1.1.3) the stability and catalytic activity of lipase at the bead-water interface are governed by the same two ionizable groups with pka values (in the absence of cofactors) of 5.6 and 9.3. colipase alone or with bile salt caused only a slight p ... | 1976 | 1388 |
| interference by mepacrine with the storage of 5-hydroxytryptamine in blood platelets. | | 1975 | 2676 |
| distribution of prostaglandin e 9-ketoreductase and nad+-dependent and nadp+-dependent 15-hydroxyprostaglandin dehydrogenase in the renal cortex and medulla of various species. | regional distributions of pge 9-ketoreductase and 15-hydroxy-prostaglandin dehydrogenase were examined in the cytoplasmic fractions from the kidneys of seven species. all species contained an nadph-dependent reductase, as well as nad+- and nadp+-dependent dehydrogenases in both cortex and medulla. a previously unrecognized cytoplasmic nadh-dependent pge 9-ketoreductase was also detected in the cortex and medulla of rat and bovine kidney. total nad+- and nadp+-dependent dehydrogenase activity was ... | 1975 | 2961 |
| studies on the release of plasminogen activator. | | 1976 | 2993 |
| effects of lethal overexposure on gastric secretion in the pig. | the characteristics of gastric secretion following acute gamma exposure (1500 rd) were studied in pigs with a small pavlov stomach. spontaneous secretion was practically inhibited when the animals were taken out of the irradiation cell. it slowly recovered in the following hours and its ph, first neutral, turned acid again. however, till the third day, it did not recover normal characteristics. the excretion of sodium ions was much increased. under histaminic stimulation, the responses of irradi ... | 1975 | 2996 |
| effects of acidosis on myocardial contractility and metabolism. | | 1976 | 3100 |
| a simple and efficient method for the preparation of gdp-fucose. | | 1975 | 3122 |
| porcine malignant hyperthermia induced by halothane and succinylcholine: failure of treatment with procaine or procainamide. | metabolic, hemodynamic and neuroendocrine responses to the combined use of halothane and succinylcholine (sch) were measured in five normal swine and five swine susceptible to malignant hyperthermia (mh). constant-volume ventilation was used, and no therapy was instituted. the overall response in susceptible swine was fulminant, in that it involved the rapid onset of sch-induced mh combined with the more severe metabolic, endocrine, and cardiovascular effects of halothane-induced mh. maximal cha ... | 1976 | 3127 |
| role of nadph in the regulation of nadp-specific isocitrate dehydrogenase from pig heart. | | 1976 | 3141 |
| recognition at cell surfaces: phytohaemagglutinin-lymphocyte interaction. | many aspects of cell behaviour are regulated by the interaction of extracellular ligands with specific receptors exposed on the cell surface. the receptors correspond to membrane proteins and expecially glycoproteins. a key event in regulation is the transmission across the surface membrane of the information resulting from receptor-ligand interaction. the activation of lymphocytes by phaseolus vulgaris phytohaemagglutinin (pha) provides a convenient experimental model for the study of the molec ... | 1975 | 1813 |
| enhancement of the antigenic activity and virulence of the vaccine strain e of rickettsia prow azeki by passages in cell culture. | changes in the biological properties of the vaccine strain e of rickettsia prowazeki occurred upon cultivation of a1 (human amnion) cells infected with this strain. in the course of passages of these cells the antigenic activity and virulence of the rickettsia increased. the changes were observed in 10 out of 22 cell cultures examined: in 6 cultures there was an increase in the antigenic activity and in 4 both in the antigenic activity and in virulence. the time of the occurrence of these change ... | 1975 | 1993 |
| pig kidney epithelial (ps) cells: a perfect tool for the study of flaviviruses and some other arboviruses. | | 1975 | 1999 |
| the effect of sulfhydryl reagents on the heart rate and coronary flow of the isolated perfused guinea-pig heart. | the action of a number of compounds able to react with thiols was tested on guinea-pig hearts perfused at constant pressure. the sh reagents used were nano2, oxidized glutathione, cystamine, diamide, 1,5-difluoro-2,4-dinitrobenzene, nitroglycerol, sodium nitroferricyanide and hgcl2. 6,6'-dithiodinicotinic acid, an sh reagent that does not penetrate the cell, produced no effect. all the other sh reagents produced an increase in coronary flow. all except oxidized glutathione and nitroglycerol incr ... | 1975 | 2119 |
| action of six commonly used benzodiazepines on isolated guinea-pig ileum preparation. | the spasmolytic activity of six commonly used benzodiazepines was investigated on isolated guinea-pig ileum preparation. all six substances proved to be non-competitive antagonists of carbachol and barium chloride, the pd'2 values ranging between 3.23 and 4.37 in the presence of either agonist. the significance of these findings is discussed. | 1975 | 2123 |
| the influence of ph on the growth and stability of transmissible gastroenteritis virus in vitro. | the influence of ph on the growth of transmissible gastroenteritis virus (tgev) in adult pig thyroid cell culture, and on the stability of the virus was studied. at ph 7.2 and 100 fold higher than those at ph 8.0. the adsorption, penetration and uncoating steps of the viral replicative cycle were shown to be unaffected by ph variation. synthesis of tgev rna during the first 12 hours post infection was found to be unaffected by ph variation between the range 6.5-8.0. after 12 hours breakdown of t ... | 1975 | 2142 |
| calcitonin-sensitive adenylate cyclase in rat renal tubular membranes. | 1. renal tubular membranes from rat kidneys were prepared, and adenylate cyclase activity was measured under basal conditions, after stimulation by naf or salmon calcitonin. apparent km value of the enzyme for hormone-linked receptor was close to 1 x 10(-8) m. 2. the system was sensitive to temperature and ph. ph was found to act both on affinity for salmon calcitonin-linked receptor and maximum stimulation, suggesting an effect of ph on hormone-receptor binding and on a subsequent step. 3. kcl ... | 1975 | 2153 |
| the structure of the covalent adduct formed by the interaction of 3-dimethylamino-1-propyne and the flavine of mitochondrial amine oxidase. | 3-dimethylamino-1-propyne irreversibly inactivates mitochondrial monoamine oxidase from bovine liver. the inactivation results in the loss of absorption in the 450-500-nm region of the flavine spectrum and a concomitant increase in absorbance at 410 nm. for the enzyme-bound adduct epsilon410 = 28000. the spectral properties of the adduct of the liver enzyme with 3-dimethylamino-1-propyne are similar to those observed when the pig kidney enzyme is inactivated with pargyline (chuang et al. (1974), ... | 1976 | 2278 |
| interaction of antihistaminic agents with adrenoceptors. | | 1975 | 2553 |
| toxic properties of regenerated water containing methyl alcohol. | the experiments carried out on white male rats have shown no significant changes in the animals that may have resulted from their 30-day consumption of reclaimed water which contained up to 34 mg/l methanol and was produced by sorption purification of the atmospheric condensate during a prolonged manned experiment. | 1975 | 1578 |
| xenograft in the treatment of burns. | the injury due to burns embraces three, nearly concentric zones. the necrobiosis of the central and marginal zones is reversible and the pig-skin - assuring biological ligature - plays important role in turning for better of the process. the preparation and use of xenograft - applied in the author's department - is discussed. | 1975 | 1600 |
| conformation of gonadotropin releasing hormone. | the conformation of the gonadotropin releasing hormone (gn-rh), whose primary sequence is pglu-his-trp-ser-tyr-gly-leu-arg-pro-glynh2, and of several of its structural analogues has been studied by circular dichroism, optical rotatory dispersion, and fluorescence spectroscopy. the effects of ph, guanidine, and temperature on fluorescence emission have also been examined. titration data demonstrate that the histidine and tyrosine residues are free of any mutual interactions. the similarity of emi ... | 1976 | 2282 |
| fluorescence polarization studies on the binding between glutamate dehydrogenase and cytoplasmic aspartate aminotransferase. | polarization of fluorescence measurements on aspartate aminotransferase (from pig heart cytosol) labeled with fluorescein isothiocyanate have been used to detect the formation of a soluble complex of this protein wich glutamate dehydrogenase from bovine liver. the binding of the labeled transaminase to dehydrogenase is detectable at catalytic concentrations of the enzymes. | 1975 | 3487 |
| acid secretion by guinea-pig isolated stomach. | an isolated stomach preparation from the guinea-pig is described. 2. both histamine acid phosphate (1-4 mug/ml.) and theophylline hydrate (0-2-3-2 mg/ml.) separately stimulated hydrochloric acid, hcl, secretion from the guinea-pig stomach preparation. a linear dose-response relationship was obtained for theophylline. 3. addition of theophylline (0-2 and 1-6 mg/ml.) during maximal response to histamine increased the secretion further, whereas addition of histamine during maximal response to theop ... | 1976 | 3644 |
| drug-induced phospholipidoses. ii. tissue distribution of the amphiphilic drug chlorphentermine. | | 1975 | 2448 |
| action of h1 and h2 inhibitors on the response of histamine sensitive adenyly cyclase from guinea-pig mucosa. | in the guinea-pig, it has been shown that homogenates of mucosa from the fundus contain an adenylyl cyclase system that is activated by histamine as well as by prostaglandins pge1 and pga1. the effects of burimamide, an h2-inhibitor, and mepyramine and chlorpheniramide, both h1-inhibitors, were tested. both h1 and h2 inhibitors behaved kinetically as competitive inhibitors of histamine, but the km derived for burimamide (2.5 - 4.1 . 10(-5)) was significantly lower than that for either chlorpheni ... | 1976 | 3419 |
| fenitrooxon and s-methyl fenitrothion: acute toxicity and hydrolysis in mammals. | | 1975 | 2462 |
| effect of ischemic anoxia on electrical and mechanical activity of the totally isolated porcine stomach. | electrical and mechanical activity were recorded from totally isolated whole porcine stomachs perfused with homologous blood. stagnant, complete ischemia was then produced by closing the gastric artery and vein for various periods of time up to 3 h. after a given period of ischemia, blood was recirculated for 10 min and then pentagastrin was injected into the gastric artery. the vagus nerve was also electrically stimulated at various stages of anoxia and recirculation. after up to 90 min of isch ... | 1976 | 2479 |
| combined azaperone and metomidate anaesthesia in liver transplantation in the pig. | combined azaperone and metomidate anaesthesia has been used in 86 surgical procedures on 84 piglets, either as such or deepened and prolonged. 51 animals were sacrificed at the end of the procedure as planned before. the anesthesia allowed the performance of various short and long operations. out of the 33 remaining pigs, submitted to 35 operations, 4 died during the procedure of a technical fault; 2 did not recover from a deep hypothermia (below 10 degrees c); 1 died from the hepatic coma induc ... | 1976 | 2480 |
| boar acrosin, ii: amino acid composition, amino terminal residue and molecular weight estimations by ultracentrifugation. | the molecular weight of boar acrosin in neutral solution was estimated to be 41000 +/- 1000 by high-speed sedimentation equilibrium analysis. this result is in good agreement with the value found earlier1 by sodium dodecylsulfate polyacrylamide gel electrophoresis. the sedimentation coefficeint of acrosin obtained by active enzyme centrifugation of partly purified preparations is in accordance with the sedimentation coefficient of the pure preparation estimated by conventional sedimentation velo ... | 1975 | 2533 |
| inhibition by superoxide dismutase of methemoglobin formation from oxyhemoglobin. | the formation of methemoglobin from oxyhemoglobin in a solution containing photoreduced riboflavin and oxygen was inhibited by superoxide dismutase. the rate of the reaction was ph-dependent in the range of 6.8 to 7.8, increasing as the ph was reduced. inhibition by superoxide dismutase was enhanced as the edta concentration increased and was dependent on enzymatic activity. under conditions in which superoxide dismutase inhibition was incomplete, catalase inhibited the reaction but mannitol had ... | 1976 | 2597 |
| demonstration of a precursor-product relationship between soluble and cross-linked elastin, and the biosynthesis of the desmosines in vitro. | direct evidence showing that a soluble form of elastin is the precursor of cross-linked elastin was obtained from pulse-chase experiments using chick embryo aortas and by demonstrating the conversion of soluble elastin into cross-linked elastin in a cell-free system. acetic acid extracts of embryonic chick aorta pulse-labeled with 14clysine contain two radioactive proteins of molecular weights 74,000 and 138,000 which have been identified previously as soluble elastin and the pro-alpha chain of ... | 1976 | 2602 |
| the identification and subcellular localization of thrombosthenin "m", the myosin-like component of pig platelets. | a protein has been studied which spontaneously precipitates from stored fractions of platelet soluble phase prepared by density gradient centrifugation. it is rich in a ca2+ atpase activity which displays an activity/ph profile resembling that of skeletal muscle myosin. adjustment of freshly prepared soluble phase fractions to 0.6 m with respect to kcl and dilution 1 in 3 results in the precipitations of a protein fraction with essentially the same enzymatic properties as the spontaneously preci ... | 1975 | 2643 |
| kinetic properties of pulmonary angiotensin-converting enzyme. hydrolysis of hippurylglycylglycine. | some of the kinetic properties of angiotensin-converting enzyme (peptidyl-dipeptide hydrolase, ec 3.4.15.1) purified from hog lung have been determined using hippurylglycylglycine as substrate. the effects of ph and ionic environment on enzyme activity are complex and interdependent. at 0.1 m nacl, the ph-activity curve shows an abrupt decrease in v/km as the ph rises from 6 to 6.5, implying that ionization of a group in the enzyme with a pk in this range aids in binding of the substrate. chlori ... | 1976 | 4120 |
| alkylation of cysteinyl residues of pig heart nad-specific isocitrate dehydrogenase by iodoacetate. | pig heart nad-specific isocitrate dehydrogenase is inactivated by reaction with iodoacetate at ph 6.0. loss of activity can be attributed to the formation of 1-2 mol of carboxymethyl-cysteine per peptide chain. the rate of inactivation is markedly decreased by the combined addition of mn2+ and isocitrate, but not by alpha-ketoglutarate, the coenzyme nad or the allosteric activator adp. the substrate concentration dependence of the decreased rate of inactivation yields a dissociation constant of ... | 1976 | 4125 |
| cerebral monoamine metabolism in guinea-pigs with ascorbic acid deficiency. | guinea-pigs kept on a diet deficient in vitamin c showed, after 3 weeks, a marked decrease of ascorbic acid in brain and blood leucocytes as well as of the activity of alkaline phosphatase in blood plasma. pair-fed animals did not exhibit these changes. the alpha-methyl-p-tyrosine (alpha mpt)-induced diminution of noradrenaline in the hypothalamus and the rest of the brain was attenuated in pair-fed animals, but restored in guinea-pigs deficient in ascorbic acid. the cerebral noradrenaline conte ... | 1975 | 2659 |
| proceedings: an interaction between the alpha and beta actions of catecholamines in guinea-pig liver slices. | | 1976 | 4183 |
| proceedings: influence of a mucin model system upon the bioavailability of phenylbutazone and warfarin sodium from the small intestine. | | 1975 | 2751 |
| polyarteritis nodosa: general aspects and occurrence in domestic animals, particularly in association with nosematosis in blue foxex (author's transl). | a survey of polyarteritis nodosa in domestic animals is presented, including a description of the patho-morphological lesions and a discussion on the causal factors. this vascular disease occurs in domestic animals in association with both viral infections (infectious plasmacytosis in mink), bacterial infections (erysipelas in swine), and protozoan infections (nosematosis in blue foxes). the conclusion is drawn that the observations in domestic animals support the view that immunological disturb ... | 1976 | 2901 |
| caesarean section in sows anesthetized with azaperone and metomidate (author's transl). | 213 caesarean sections and 157 hysterectomies were carried out in gilts and sows with different body weight (table i). the neuroleptic azaperone and the hypnotic metomidate were used for anaesthesia with different administration (table ii). local analgesia and premedication with atropine. the duration of the anaesthesia was 45 minutes and where prolongation was necessary, metomidate, azaperone or barbiturates were used alone or in combinations once or more. the indications (table iii) for caesar ... | 1976 | 2902 |
| the effect of acid proteinase inhibitors on chicken pepsin. | 1. the activity of chicken pepsin was partially inhibited by dimethyl-(2-hydroxy-5-nitrobenzyl)sulphonium bromide, but was unaffected by p-bromophenacyl bromide. 2. in the presence of cu2+, diazoacetylnorleucine methyl ester completely inactivated chicken pepsin with the incorporation of 1 mol/mol. the mechanism of the reaction was similar to that with pig pepsin. 3. chicken pepsin was completely inactivated by 2-diazo-4-bromoacetophenone in the presence of cu2+. 4. chicken pepsin was almost com ... | 1975 | 3165 |