Publications

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systemic mycobacterium marinum infection in an amazon manatee. 19854077647
noninvasive monitoring of androgens in male amazonian manatee (trichechus inunguis): biologic validation.the amazonian manatee (trichechus inunguis) is endemic in the amazonian basin and is the only exclusively fresh water sirenian. historically hunted on a large scale, this species is now considered endangered, and studies on the reproductive physiology are critical for the improvement of reproductive management of captive and wild populations of manatees. the aim of this study was to verify the viability of androgen measurement in saliva, lacrimal, urine, and fecal samples of the amazonian manate ...200919746860
the primary structure of the hemoglobin of the brazilian manatee (trichechus inunguis, sirenia).the hemoglobin of the brazilian manatee (trichechus inunguis, sirenia) consists of one component. we present the primary structures of the alpha- and beta-chains which have been separated by chromatography on carboxymethyl-cellulose cm-52. the sequences have been determined by automatic edman degradation with the film technique, using the native chains, tryptic peptides and the c-terminal prolyl-peptide obtained by acid hydrolysis of the asp-pro bond of the alpha-chains. compared to the correspo ...19883202957
acute necrotizing colitis with pneumatosis intestinalis in an amazonian manatee calf.on 25 january 2014, a 1 mo old female amazonian manatee trichechus inunguis calf weighing 12 kg was rescued by air transport in guajará, brazil, and transferred to mamirauá institute's community-based amazonian manatee rehabilitation center. the calf presented piercing/cutting lesions on the back, neck, and head, in addition to dehydration and intermittent involuntary buoyancy. x-ray analysis revealed a large amount of gases in the gastrointestinal tract. daily procedures included wound cleaning ...201627503914
seasonal variation in urinary and salivary reproductive hormone levels in amazonian manatees (trichechus inunguis).the amazonian manatee (trichechus inunguis) is a threatened aquatic mammal endemic to the amazon basin. the aim of this study was to evaluate the urinary and salivary reproductive hormone levels of captive amazonian manatees collected during two seasons of the year. salivary samples from four males and urinary and salivary samples from three females were collected during two seasons (march-june and september-november) over two consecutive years. salivary testosterone in males was measured by rad ...201524690451
yeast microbiota of natural cavities of manatees (trichechus inunguis and trichechus manatus) in brazil and its relevance for animal health and management in captivity.the aim of this study was to characterize the yeast microbiota of natural cavities of manatees kept in captivity in brazil. sterile swabs from the oral cavity, nostrils, genital opening, and rectum of 50 trichechus inunguis and 26 trichechus manatus were collected. the samples were plated on sabouraud agar with chloramphenicol and incubated at 25 °c for 5 days. the yeasts isolated were phenotypically identified by biochemical and micromorphological tests. overall, 141 strains were isolated, of w ...201526308797
occurrence of antibodies to toxoplasma gondii and lepstospira spp. in manatees (trichechus inunguis) of the brazilian amazon.the presence of toxoplasma gondii and leptospira spp. antibodies was investigated in 74 manatees (trichechus inunguis [mammalia: sirenia]) kept in captivity in two rescue units in the northern region of brazil. antibodies to t. gondii were detected in 29 (39.2%) of 74 animals by using the modified agglutination test (titer, 1:25). for antibodies against leptospira spp., sera were diluted 1:50 and tested against 24 strains ofleptospires by microscopic agglutination microtechnique, and positive sa ...201222448513
sleep in an amazonian manatee, trichechus inunguis.for the first time, sleep was studied in a representative of the order of sirenia. slow wave sleep occupied 27%, and paradoxical sleep 1% of the total recording time in the amazonian manatee. trichechus inunguis. the circadian rhythmicity of sleep was pronounced. during the sleep period, the manatee woke up for a short time for each respiratory act. interhemispheric asynchrony of the electrocortical slow wave activity was found.19921582500
comments on "intraspecific and geographic variation of west indian manatee (trichechus manatus spp.) vocalizations" [j. acoust. soc. am. 114, 66-69 (2003)].this letter concerns the paper "intraspecific and geographic variation of west indian manatee (trichechus manatus spp.) vocalizations" [nowacek et al., j. acoust. soc. am. 114, 66-69 (2003)]. the purpose here is to correct the fundamental frequency range and information on intraindividual variation in the vocalizations of amazonian manatees reported by nowacek et al. (2003) in citing the paper "signature information and individual recognition in the isolation calls of amazonian manatees, trichec ...200616838493
reproductive anatomy of the female amazonian manatee trichechus inunguis natterer, 1883 (mammalia: sirenia).the amazonian manatee (trichechus inunguis) is uniparous and has a slow reproduction cycle due to a long gestation period and long interval between births. even though protected by law, hunting remains one of the main causes hindering the natural population growth of this species in the wild. the histology and reproductive anatomy provide information on the history and reproductive status of the female and offer a tool for the conservation of the species. the present study describes the anatomy ...200818383272
placentation in the amazonian manatee (trichechus inunguis).evidence from several sources supports a close phylogenetic relationship between elephants and sirenians. to explore whether this was reflected in similar placentation, we examined eight delivered placentae from the amazonian manatee using light microscopy and immunohistochemistry. in addition, the fetal placental circulation was described by scanning electron microscopy of vessel casts. the manatee placenta was zonary and endotheliochorial, like that of the elephant. the interhaemal barrier com ...200818462617
urinary parameters of trichechus inunguis (mammalia, sirenia): reference values for the amazonian manatee.the amazonian manatee, trichechus inunguis (natterer 1883) is endemic to the amazon basin and is currently considered a vulnerable species. in order to establish normality ranges of urinary parameters to help monitor the health of this species in captivity, chemical urinalyses were performed on twelve males and nine females of various age groups. urine was collected once a month for twelve months in the tanks just after being drained, by placing stainless steel containers under the genital slit ...201020730348
serum biochemical analytes in captive amazonian manatees (trichechus inunguis).establishment of reference values for serum biochemical analytes is important for monitoring health and physiological status of captive animals.201121366658
occurrence of cryptosporidium spp. in antillean manatees (trichechus manatus) and amazonian manatees (trichechus inunguis) from brazil.infections by cryptosporidium spp. in aquatic mammals is a major concern due to the possibility of the waterborne transmission of oocysts. the aim of the present study was to report the occurrence of cryptosporidium spp. in antillean manatees (trichechus manatus) and amazonian manatees (trichechus inunguis) from brazil. fecal samples were collected and processed using kinyoun's method. positive samples were also submitted to the direct immunofluorescence test. the results revealed the presence o ...201122204053
phylogeography and sex-biased dispersal across riverine manatee populations (trichechus inunguis and trichechus manatus) in south america.phylogeographic patterns and sex-biased dispersal were studied in riverine populations of west indian (trichechus manatus) and amazonian manatees (t. inunguis) in south america, using 410bp d-loop (control region, mitochondrial dna) sequences and 15 nuclear microsatellite loci. this multi-locus approach was key to disentangle complex patterns of gene flow among populations. d-loop analyses revealed population structuring among all colombian rivers for t. manatus, while microsatellite data sugges ...201223285054
the mammary glands of the amazonian manatee, trichechus inunguis (mammalia: sirenia): morphological characteristics and microscopic anatomy.the mammaries from carcasses of two female amazonian manatees were examined. trichechus inunguis possesses two axillary mammaries beneath the pectoral fins, one on each side of the body. each papilla mammae has a small hole on its apex--the ostium papillare. the mammaries are covered by a stratified squamous keratinized epithelium. the epithelium of the mammary ducts became thinner more deeply in the tissue and varied from stratified to simple cuboidal. there was no evidence of glandular activit ...201424920139
first attempt to monitor luteinizing hormone and reproductive steroids in urine samples of the amazonian manatee (trichechus inunguis).the aims of this study were to validate an enzyme immunoassay (eia) for the measurement of luteinizing hormone (lh) in urine samples of amazonian manatees (trichechus inunguis; mammalia: sirenia) and to monitor urinary lh and reproductive steroids during the ovarian cycle in this species. urine samples were collected from two captive males following a hormonal challenge with a gonadotropin-releasing hormone (gnrh) analogue. the urinary lh results from hormonal challenge were compared with urinar ...201425632672
antifungal resistance and virulence among candida spp. from captive amazonian manatees and west indian manatees: potential impacts on animal and environmental health.this work aimed at evaluating the antifungal susceptibility and production of virulence factors by candida spp. isolated from sirenians in brazil. the isolates (n = 105) were recovered from the natural cavities of amazonian and west indian manatees and were tested for the susceptibility to amphotericin b, itraconazole, and fluconazole and for the production of phospholipases, proteases, and biofilm. the minimum inhibitory concentrations (mics) for amphotericin b ranged from 0.03 to 1 µg/ml, and ...201626813966
phylogeography of the west indian manatee (trichechus manatus): how many populations and how many taxa?to resolve the population genetic structure and phylogeography of the west indian manatee (trichechus manatus), mitochondrial (mt) dna control region sequences were compared among eight locations across the western atlantic region. fifteen haplotypes were identified among 86 individuals from florida, puerto rico, the dominican republic, mexico, columbia, venezuela, guyana and brazil. despite the manatee's ability to move thousands of kilometers along continental margins, strong population separa ...19989734072
phylogeography and population genetics of the endangered amazonian manatee, trichechus inunguis natterer, 1883 (mammalia, sirenia).we used mitochondrial dna control region sequences to examine phylogeography and population differentiation of the endangered amazonian manatee trichechus inunguis. we observe lack of molecular differentiation among localities and we find weak association between geographical and genetic distances. however, nested clade analysis supports restricted gene flow and/or dispersal with some long-distance dispersal. although this species has a history of extensive hunting, genetic diversity and effecti ...200515660933
heart rates and gas exchange in the amazonian manatee (trichechus inunguis) in relation to diving.unrestrained amazonian manatees (trichechus inunguis) maintained a constant heart rate during diving and exhibited a slight tachycardia during breathing. 'forcing' the manatees to dive caused a marked bradycardia. they exhibited a more pronounced tachycardia during breathing after 'forced' dives and hyperventilated during recovery dives. manatees are capable of dives exceeding 10 min duration without having to resport to anaerobic metabolism, and even after 10 min dives recover within 3-4 short ...19863088073
phylogeography, phylogeny and hybridization in trichechid sirenians: implications for manatee conservation.abstract the three living species of manatees, west indian (trichechus manatus), amazonian (trichechus inunguis) and west african (trichechus senegalensis), are distributed across the shallow tropical and subtropical waters of america and the western coast of africa. we have sequenced the mitochondrial dna control region in 330 trichechus to compare their phylogeographic patterns. in t. manatus we observed a marked population structure with the identification of three haplotype clusters showing ...200616448411
complete mitochondrial genome of the florida manatee (trichechus manatus latirotris, sirenia).the florida manatee (trichechus manatus latirostris) is an endangered subspecies of the west indian manatee (t. manatus), which inhabits inland and marine waters of southeastern united states. in this study, we assembled the mitochondrial genome (mtdna) of the florida manatee from whole genome shotgun reads. as a result, we show that the currently annotated t. manatus mtdna belongs to a different species, the amazonian manatee (t. inunguis). the newly assembled florida manatee mtdna is 16,881 bp ...202032142097
cutaneous mycobacteriosis in a captive amazonian manatee trichechus inunguis.an adult male amazonian manatee trichechus inunguis under human care presented with 3 circular cutaneous lesions on the dorsal aspect of the rostrum and between the nostrils (plenum). initially these lesions were superficial, hypopigmented, without warmth and non-painful. microbiological cultures of skin swabs isolated candida sp. and pseudomonas aeruginosa, and topical treatment with antiseptic, antifungal, anti-inflammatory and antibiotic medication was instituted. this treatment strategy did ...201829516862
resource partitioning among stranded aquatic mammals from amazon and northeastern coast of brazil revealed through carbon and nitrogen stable isotopes.aquatic mammals play an important role in community structure. the present study applied stable isotope analysis (sia) to evidence trophic relationships and resource partitioning among aquatic mammals inhabiting different environments in the amazon estuarine complex and adjacent coastal zone (ae) and northeastern coast (nc) of brazil. in addition, isotopic niche partitioning among sotalia guianensis, inia spp. and trichechus inunguis within the ae was also evaluated, and ecological s. guianensis ...202032732987
endocrine monitoring of the ovarian cycle in captive female amazonian manatees (trichechus inunguis).the amazonian manatee (trichechus inunguis; mammalia: sirenia), a threatened aquatic mammal endemic to the amazon basin, is the only sirenian that lives exclusively in fresh water. information about the reproductive endocrinology of the amazonian manatee is scarce; therefore, the aim of this study was to monitor salivary progesterone and estradiol patterns during the ovarian cycle in t. inunguis. salivary samples were collected daily during a 12-week period of two consecutive years from two capt ...201324075242
cryptosporidium spp. and giardia sp. in aquatic mammals in northern and northeastern brazil.cryptosporidium and giardia are protozoans that can infect humans and wild and domestic animals. due to the growing importance of diseases caused by protozoan parasites in aquatic species, we aimed to evaluate the frequency of infection by cryptosporidium spp. and giardia sp. in aquatic and marine mammals in the northern and northeastern regions of brazil. we collected 553 fecal samples from 15 species of wild-ranging and captive aquatic mammals in northern and northeastern brazil. all samples w ...201728930082
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