pathogenicity attributed to massive infection of nanophyetus salmincola in a estimated 650,000 nanophyetus salmincola were recovered from the small intestine of a wild female cougar kitten (felis concolor). the trematodes stimulated marked mucosal thickening in the duodenum and jejunum and caused a marked enteritis. malnutrition was listed as the cause of death due to impaired nutrient absorption resulting from the combined effects of the diarrhea and thickening of the intestine.1979501847
a skeletal growth defect in the puma (felis concolor).clinical and radiological signs shown by a young deformed puma cub, and the pathological findings following its euthanasia at eight months, are presented. these findings do not resemble those in the recognised nutritional bone diseases of the growing carnivore, but closely resemble an hereditary disease of the dog and, at least in one respect, an hereditary disease in man. this case may be an example of hereditary chondrodysplastic dwarfism in the puma. an unusual finding was the presence of iro ...1978734877
spongiform encephalopathy in a captive puma (felis concolor).a captive adult puma developed ataxia, a hypermetric gait and whole body tremor. the signs progressed over a period of six weeks. histopathological examination following euthanasia demonstrated spongiform encephalopathy, gliosis and mild non-suppurative meningoencephalitis. immunostaining with a polyclonal antiserum revealed prion protein (prp) associated with these changes in sections of cervical spinal cord and medulla. this is the first confirmed case of a scrapie-like spongiform encephalopat ...19921455592
gastrointestinal parasites of cougars (felis concolor) in washington and the first report of ollulanus tricuspis in a sylvatic felid from north america.gastrointestinal helminths including two species of cestodes (taenia omissa and t. ovis krabbei) and three species of nematodes (toxocara cati, cylicospirura subequalis and ollulanus tricuspis) are reported from two free-ranging cougars (felis concolor) in washington (usa). ollulanus tricuspis is reported for the first time from cougars and represents the first occurrence of this parasite in a sylvatic felid from north america.19921548792
amyloid in the pancreatic islets of the cougar (felis concolor) is derived from islet amyloid polypeptide (iapp).1. islet amyloid isolated from the pancreas of a 20-year-old cougar (felis concolor) was dissolved and purified by gel permeation and reversed phase hplc for amino acid sequence analysis. 2. n-terminal amino acid sequence analysis of the purified protein revealed a primary structure (positions 1-28) identical to islet amyloid polypeptide (iapp) from domesticated cats. 3. iapp from the cougar, like iapp from the human and domesticated cat, incorporates an inherently amyloidogenic ails sequence at ...19912060275
iatrogenic transmission of cytauxzoon felis from a florida panther (felix concolor coryi) to a domestic cat.a laboratory cat died 12 days after intraperitoneal inoculation of a 1 ml suspension containing 1.5 x 10(6) blood mononuclear cells from a florida panther (felis concolor coryi). gross, histologic and ultrastructural investigations revealed the cause of death to be infection by cytauxzoon felis, a protozoal parasite known to cause a rapidly fatal disease (cytauxzoonosis) in domestic cats. the bobcat (felis rufus) has been identified as a natural host for c. felis. this report implicates the flor ...19912067059
sarcocystis sp. in muscles of free-ranging florida panthers and cougars (felis concolor).sarcocysts of sarcocystis sp. were found in the striated muscles from 11 of 14 wild florida panthers (felis concolor coryi) and four of four cougars (two wild f. concolor stanleyana and two captive f. concolor of undetermined subspecies). the common occurrence of sarcocysts in muscles of top carnivores such panthers and cougars is unexplained. this stage of the life cycle is normally confined to the muscles of the prey species. because large felids are rarely preyed upon, it is unlikely that a s ...19892509739
immobilizing wild mountain lions (felis concolor) with ketamine hydrochloride and xylazine hydrochloride.a mixture of 120 mg ketamine hydrochloride (khcl)/20 mg xylazine hydrochloride (xhcl)/ml was used to immobilize 37 wild mountain lions (felis concolor) 46 times. observations were recorded during 37 trials that included kittens, adult females, and adult males. dosages were based on 11 mg khcl and 1.8 mg xhcl/kg estimated body weight. actual doses for 24 lions requiring a single injection for immobilization ranged from 4.7-15.8 mg khcl/kg and 0.8-2.6 mg xhcl/kg. induction, duration, and recovery ...19863951066
[presence in costi rica of echinococcus oligarthrus diesing 1863, collected in the small intestine of felis concolor costaricensis]. 19734781534
prevalence and distribution of larvae of trichinella sp. in cougars, felis concolor l., and grizzly bears, ursus arctos l., in alberta. 19846492330
sarcocystis and other coccidia in foxes and other wild carnivores from montana.sarcocystis spp sporocysts were found in feces of 10.1% of 198 red foxes (vulpes vulpes), in 3.2% of 61 bobcats (lynx rufus), in 16.6% of 12 mountain lions (felis concolor), in 16.6% of 6 fisher (martes pennanti), and in none of 20 wolverines (gulo gulo), 4 mink (mustela vison), or 10 raccoons (procyon lotor). sarcocystis muris and toxoplasma gondii were not found in laboratory mice inoculated with feces of bobcats and mountain lions.19826816776
successful induced ovulation and artificial insemination in the puma (felis concolor). 19817269176
molecular evolution of mitochondrial 12s rna and cytochrome b sequences in the pantherine lineage of felidae.dna sequence comparisons of two mitochondrial dna genes were used to infer phylogenetic relationships among 17 felidae species, notably 15 in the previously described pantherine lineage. the polymerase chain reaction (pcr) was used to generate sequences of 358 base pairs of the mitochondrial 12s rna gene and 289 base pairs of the cytochrome b protein coding gene. dna sequences were compared within and between 17 felid and five nonfelid carnivore species. evolutionary trees were constructed using ...19957544865
epidemiological observations on spongiform encephalopathies in captive wild animals in the british isles.since 1986, scrapie-like spongiform encephalopathy has been diagnosed in 19 captive wild animals of eight species at or from eight zoological collections in the british isles. the affected animals have comprised members of the family bovidae: one nyala (tragelaphus angasi), four eland (taurotragus oryx), and six greater kudu (tragelaphus strepsiceros), one gemsbok (oryx gazella), one arabian oryx (oryx leucoryx), and one scimitar-horned oryx (oryx dammah), and members of the family felidae: four ...19947817514
isolation of pseudorabies (aujeszky's disease) virus from a florida panther.pseudorabies virus was isolated in cell culture from the brain tissue of a 3.5-year-old male florida panther (felis concolor coryi). the virus was not isolated from other tissues collected at necropsy. based upon a nested polymerase chain reaction (pcr), the virus was determined to have the classical wild-type virulent genotype, glycoprotein i+ (gi+) and thymidine kinase+ (tk+).19948028102
serologic survey and serum biochemical reference ranges of the free-ranging mountain lion (felis concolor) in california.serum samples from 58 mountain lions (felis concolor) in california (usa) were collected between april 1987 and february 1990. nineteen serum samples were used for serum biochemistry determinations; the ranges were similar to reference values in domestic cats, captive exotic felidae and free-ranging mountain lions. a serological survey was conducted to determine whether antibodies were present against selected infectious agents. fifty-four (93%) of 58 sera had antibodies against feline panleukop ...19948028105
nucleotide sequence analysis of puma lentivirus (plv-14): genomic organization and relationship to other lentiviruses.the complete nucleotide sequence of an isolate of puma lentivirus (plv-14) was obtained by an inverse polymerase chain reaction (i-pcr) technique and confirmed by conventional pcr. both methods were used to amplify overlapping regions of proviral dna, for cloning and sequencing, from genomic dna isolated from plv-14 infected florida puma (felis concolor coryi) peripheral blood mononuclear cells (pbmc). the provirus has a total length of 9100 nucleotides and the genomic organization of presumed p ...19948030248
gonadotrophin dose and timing of anaesthesia for laparoscopic artificial insemination in the puma (felis concolor).ovarian response to equine chorionic gonadotrophin (ecg) and human chorionic gonadotrophin (hcg), the effect of timing of anaesthesia relative to hcg injection and the use of laparoscopic intrauterine artificial insemination were examined in the puma (felis concolor). in expt 1, females were treated with 100 (n = 6) or 200 (n = 8) iu ecg (i.m.) followed 80 h later by 100 iu hcg (i.m.) and were then anaesthetized 40-43 h after hcg injection for ovarian assessment. although there was no difference ...19948064667
seroprevalence of infectious disease agents in free-ranging florida panthers (felis concolor coryi).serum samples obtained from 38 free-ranging florida panthers (felis concolor coryi) in southern florida, march 1978 through february 1991, were tested for antibodies against eight bacterial, parasitic, and viral disease agents. sera were positive for antibodies against feline panleukopenia virus (fpv) (78%), feline calicivirus (56%), feline immunodeficiency virus/puma lentivirus (37%), feline enteric coronavirus/feline infectious peritonitis virus (19%), and toxoplasma gondii (9%). all samples w ...19938445789
notoedric mange in the florida panther (felis concolor coryi).notoedric mange (notoedres cati) was found in a neonate florida panther (felis concolor coryi) and presumably its mother on 22 june 1992 and 8 february 1993, respectively, in collier county, florida (usa). both infestations were treated successfully with 0.2 mg/kg ivermectin. this is the first known case of notoedric mange in the endangered florida panther.19958583647
ixodid ticks from panthers and bobcats in florida.ixodid ticks were present in all 189 samples examined from 53 florida panthers (felis concolor coryi, 104 collections) and 85 bobcats (felis rufus floridana) in florida (usa) between 1974 and 1991. we identified 3,251 ticks from panthers and 918 from bobcats. specimens of dermacentor variabilis, ixodes scapularis, i. affinis, amblyomma maculatum, and a. americanum were present on 49, 39, 17, seven, and two of the 53 florida panthers, respectively, and comprised 36%, 55%, 7%, 1%, and < 1% of the ...19958592378
genetic and phylogenetic divergence of feline immunodeficiency virus in the puma (puma concolor).feline immunodeficiency virus (fiv) is a lentivirus which causes an aids-like disease in domestic cats (felis catus). a number of other felid species, including the puma (puma concolor), carry a virus closely related to domestic cat fiv. serological testing revealed the presence of antibodies to fiv in 22% of 434 samples from throughout the geographic range of the puma. fiv-pco pol gene sequences isolated from pumas revealed extensive sequence diversity, greater than has been documented in the d ...19968794304
demonstration of helicobacter pylori-like organisms in the gastric mucosa of captive exotic carnivores.samples of gastric tissue from the cardiac, fundic and pyloric region of 30 carnivores comprising 12 tigers (panthera tigris), 10 lions (panthera leo), three pumas (felis concolor), two leopards (panthera pardus), one serval (felis serval), one wolf (canis lupus) and one hyena (crocuta crocuta) kept at german zoological gardens were subjected to histopathological and immunohistochemical examination. selected tissue specimens of 12 animals were examined also by electron microscopy. the purpose of ...19979076597
occurrence of puma lentivirus infection in cougars from washington.puma lentivirus (plv) antibodies were detected in 13 (25%) of 52 serum samples obtained from cougars (felis concolor) collected by hunters. the serum samples were collected from november 1993 through january 1994 from four specific regions throughout the state of washington (usa), and included the olympic mountains, the cascade mountains, the blue mountains, and the selkirk mountains. more (38%) seropositive cougar samples originated from the cascade mountains than from any other site. the overa ...19979131566
gastric ulcer associated with a helicobacter-like organism in a cougar (felis concolor).a 12-year-old captive female cougar (felis concolor) died following perforation of a gastric ulcer. histologically, erosions and ulcers were present in the antral area of the stomach. fibroplasia and infiltrates of neutrophils bordered the ulcers. modified steiner silver stain revealed numerous tightly coiled helical bacteria. the bacteria stained positively with a rabbit polyclonal anti-helicobacter pylori antibody. morphology, location, and positive immunohistochemical staining suggests that t ...19979150547
bartonella henselae antibody prevalence in free-ranging and captive wild felids from order to determine the importance of wild felids in the epidemiology of bartonella spp. infection, 136 nobuto strips or serum samples from free-ranging mountain lions (felis concolor) and bobcats (felis rufus) captured in california (usa) between 1985 and 1996 were tested for b. henselae antibodies (titer > or = 1:64) using an immunofluorescence test. similarly, 124 serum samples from 114 captive wild cats representing 26 species or subspecies collected between 1991 and 1995 were retrieved fr ...19989476226
toxoplasma gondii in vancouver island cougars (felis concolor vancouverensis): serology and oocyst of 12 necropsied cougars (felis concolor vancouverensis) from vancouver island, british columbia, canada, shed toxoplasma gondii oocysts confirmed by mouse bioassay. eleven of the 12 cougars (92%) had antibodies to t. gondii by the modified agglutination test with titers of <1:25 (1 cougar), 1:50 (8 cougars), and 1:500 (3 cougars). one additional cougar fecal sample collected from the victoria watershed environment also contained t. gondii oocysts. in 1995, the largest reported outbreak of h ...19989576522
peritoneal coccidioidomycosis in a mountain lion in adult mountain lion (felis concolor) from the vicinity of weldon, california (usa) was necropsied following euthanasia due to emaciation and proximity to semi-rural housing. there were spherules consistent with coccidioides immitis within peritoneal surfaces with granulomatous inflammation and fungi consistent with c. immitis were cultured from abdominal fluid. this is the first reported case of coccidioidomycosis in a wild mountain lion.199910073349
potential contamination of drinking water with toxoplasma gondii oocysts.the world's first documented toxoplasmosis outbreak associated with a municipal water supply was recognized in 1995 in victoria, british columbia, canada. it was hypothesized that domestic cat (felis catus) or cougar (felis concolor) faeces contaminated a surface water reservoir with toxoplasma gondii oocysts. an extensive investigation of the victoria watershed 1 year following the outbreak documented the presence of an endemic t. gondii cycle involving the animals inhabiting the area. cats and ...199910355797
hematologic effects of cytauxzoonosis in florida panthers and texas cougars in florida.cytauxzoon felis is a long-recognized hemoparasite of free-ranging florida panthers (puma concolor coryi), but its prevalence and effect on the population has not been assessed. red blood cell indices and white blood cell counts were compared between infected and noninfected florida panthers and texas cougars (puma concolor stanleyana) from 1983-1997 in florida (usa). the prevalence of cytauxzoonosis for both populations was 39% (11/28) for texas cougars, 35% for florida panthers (22/63) and 36% ...199910479103
dermatophyte infections in free-ranging florida panthers (felis concolor coryi).three free-ranging florida panthers (felis concolor coryi) were diagnosed with clinical dermatophytosis; two were infected with trichophyton mentagrophytes, and one was infected with microsporum gypseum. two of these panthers were juvenile males that were diagnosed with focal to focally coalescing dermatophytosis; one caused by m. gypseum and the other by t. mentagrophytes. these animals were not treated, and clinical signs resolved spontaneously over 6 mo. the third panther, an adult male from ...199910484147
granulocytic ehrlichiosis and tick infestation in mountain lions in california.forty-seven mountain lions (puma concolor) collected year-round in 1996 to 1998 from the sierra nevada foothills, the northern coast ranges, and in monterey county (california, usa) were examined for infestation with ixodes pacificus and dermacentor variabilis ticks. ticks were found predominantly in winter and spring. the seroprevalence of granulocytic ehrlichiae (ge) antibodies (ehrlichia equi or the agent of human granulocytic ehrlichiosis) was 17% and the pcr-prevalence of dna characteristic ...199910574529
feline papillomas and papillomaviruses.papillomaviruses (pvs) are highly species- and site-specific pathogens of stratified squamous epithelium. although pv infections in the various felidae are rarely reported, we identified productive infections in six cat species. pv-induced proliferative skin or mucous membrane lesions were confirmed by immunohistochemical screening for papillomavirus-specific capsid antigens. seven monoclonal antibodies, each of which reacts with an immunodominant antigenic determinant of the bovine papillomavir ...200010643975
prevalence of bartonella henselae antibody in florida panthers.serum samples from 28 free-ranging florida panthers (puma concolor coryi) and seven mountain lions from texas (p. concolor stanleyana) living in south florida (usa) between 1997 to 1998 were tested for antibodies to bartonella henselae. twenty percent (7/35) of the samples were reactive to b. henselae antisera with a subspecies prevalence of 18% (5/ 28) for florida panthers and 28% (2/7) for cougars from texas (usa). there was not a significant sex related difference in infection rates among the ...200010682759
genomic ancestry of the american puma (puma concolor).puma concolor, a large american cat species, occupies the most extensive range of any new world terrestrial mammal, spanning 110 degrees of latitude from the canadian yukon to the straits of magellan. until the recent holocene, pumas coexisted with a diverse array of carnivores including the american lion (panthera atrox), the north american cheetah (miracynonyx trumani), and the saber toothed tiger (smilodon fatalis). genomic dna specimens from 315 pumas of specified geographic origin (261 cont ...200010833043
conservation value of a native forest fragment in a region of extensive agriculture.a survey of mammals and birds was carried out in a semi-deciduous forest fragment of 150 ha located in a zone of intensive agriculture in ribeirão preto, state of são paulo, south-eastern brazil. line transect sampling was used to census mammals and birds during six days, totalling 27.8 km of trails and 27.8 hours of observation. twenty mammal species were confirmed in the area (except bats and small mammals), including rare or endangered species, such as the mountain lion (puma concolor), the m ...200010959107
dietary separation of sympatric carnivores identified by molecular analysis of scats.we studied the diets of four sympatric carnivores in the flooding savannas of western venezuela by analysing predator dna and prey remains in faeces. dna was isolated and a portion of the cytochrome b gene of the mitochondrial genome amplified and sequenced from 20 of 34 scats. species were diagnosed by comparing the resulting sequences to reference sequences generated from the blood of puma (puma concolor), jaguar (panthera onca), ocelot (leopardus pardalus) and crab-eating fox (cerdocyon thous ...200011050553
trichinella infection in wildlife of the southwestern united states.several potential mammalian reservoirs of sylvatic species of trichinella were examined from texas, new mexico, and arizona. during 1998-99, tongues were collected from a black bear (ursus americanus) in arizona; from 9 black bears, a coyote (canis latrans), and a mountain lion (felis concolor) in new mexico; and from 154 coyotes, 32 raccoons (procyon lotor), 13 opossums (didelphis marsupialis), 4 ocelots (leopardus pardalis), 3 bobcats (lynx rufus), and 5 feral hogs (sus scrofa) in southern tex ...200111695403
seroprevalence of toxoplasma gondii in captive neotropical felids from brazil.seroprevalence of toxoplasma gondii was determined in 865 captive neotropical felids from 20 states from brazil, sampled from september 1995 to april 1997. sera were tested by the modified agglutination test (mat) using formalin-fixed whole tachyzoites and mercaptoethanol. antibodies (mat> or =1:20) to t. gondii were found in 472 of 865 (54.6%) cats: in 45 of 99 (45.9%) jaguarundis (herpailurus yagouaroundi), in 97 of 168 (57.7%) ocelots (leopardus pardalis), in 68 of 131 (51.9%) oncillas (l. ti ...200111777601
causes of mortality of free-ranging florida panthers.the florida panther (puma concolor coryi) is one of the most endangered mammals, with the entire population estimated to consist of only 30-50 adult animals. between 1978 and 1999, 73 free-ranging florida panther carcasses were submitted for postmortem evaluation, of which 47 (64%) were radiocollared and 26 (36%) were uncollared cats. overall, mortality of panthers > 6-mo-old was due to vehicular trauma in 25 (35%), intraspecific aggression in 19 (26%), illegal kill in seven (10%), research acti ...200211838201
gastroduodenal adenocarcinomas and rectal adenoma in a cougar (felis concolor) infected with helicobacter-like organisms and spirochetes.a 14-year-old female cougar died from gastroduodenal adenocarcinomas and rectal adenoma. at necropsy, polypoid tumor masses of various sizes were scattered on the mucosal surfaces of the stomach, duodenum, and rectum. histologically, the gastric tumor was diagnosed as an intestinal type adenocarcinoma and the tumor cells metastasized to the mesenteric lymph nodes, spleen, and lung. helicobacter-like organisms were detected in the lumina lined by foveolar epithelium. in the duodenum, the carcinom ...200211913552
bilateral eyelid agenesis repair in a captive texas cougar.bilateral eyelid agenesis was presented with multiple ocular anomalies in a captive texas cougar (felis concolor). corneal exposure resulted in substantial keratoconjunctivitis and blepharospasm. bilateral inferopapillary fundic colobomas, persistent pupillary membranes, and an atypical iris coloboma od were present. surgical repair with a rotational pedicle flap resulted in functionally effective eyelids. trichiasis was later treated with cryotherapy. eyelid agenesis is reported infrequently in ...200212236863
serum concentrations of lipids, vitamin d metabolites, retinol, retinyl esters, tocopherols and selected carotenoids in twelve captive wild felid species at four zoos.serum concentrations of several nutrients were measured in 12 captive wild felid species including caracal (felis caracal), cheetah (acinonyx jubatus), cougar (felis concolor), fishing cat (felis viverrinus), leopard (panthera pardus), lion (panthera leo), ocelot (felis pardalis), pallas cat (felis manul), sand cat (felis margarita), serval (felis serval), snow leopard (panthera uncia) and tiger (panthera tigris). diet information was collected for these animals from each participating zoo (broo ...200312514284
ticks (acari: ixodidae) on wild animals from the porto-primavera hydroelectric power station area, brazil.from june 2000 to june 2001, a total of 741 ticks were collected from 51 free-living wild animals captured at the porto-primavera hydroelectric power station area, located alongside an approximately 180 km course of the paran river, between the states of s o paulo and mato grosso do sul, comprising 9 species of 3 genera: ambly-omma (7 species), boophilus (1) and anocentor (1). a total of 421 immature amblyomma ticks were reared in laboratory until the adult stage, allowing identification of the ...200312563479
cutaneous fibropapilloma in a mountain lion (felis concolor).a 12-yr-old mountain lion (felis concolor) developed a 0.5-cm3 raised nonpigmented and nonulcerated mass between the lip and the nasal planum. the tumor was surgically removed and diagnosed histologically as a fibropapilloma. the tumor recurred 1 yr later, at which time it was again excised, and the diagnosis was reconfirmed by biopsy. frozen tissue from the second excision was submitted for polymerase chain reaction testing for papillomavirus. the 176-base pair polymerase chain reaction product ...200312885136
[the puma (felis concolor) as a new host for ancylostoma caninum (ercolani, 1859) linstow 1889]. 196414174786
domestic cats infected with lion or puma lentivirus develop anti-feline immunodeficiency virus immune responses.attenuated live viral strains have afforded significant protection against virus challenge in hiv vaccine models. although both cellular and humoral immunity are assumed to be vital for protection, specific parameters consistently associated with control of infection have been elusive. our previous studies have shown that lentiviruses from 2 nondomestic feline species--lion (pathera leo) and puma (felis concolor)--persistently but nonpathogenetically infect domestic cats (felis domestica). moreo ...200314501789
helminths of the ocelot from southern the usa, the ocelot (leopardus pardalis) is a highly endangered felid found only in a few remaining vestiges of native thornshrub brushland in the lower rio grande valley (lrgv) of extreme southern texas. from 1987-1998, carcasses of 15 adult ocelots that died of vehicular accidents or natural causes were examined for helminths. all cats had 1-8 (mean = 3) helminth species. all were infected with 1-101 (mean +/- se = 32 +/- 7) toxascaris leonina. other helminths from these ocelots were alaria ...200314567231
seroprevalence of toxoplasma gondii in american free-ranging or captive pumas (felis concolor) and bobcats (lynx rufus).toxoplasma gondii is a major zoonotic agent infecting a wide range of mammals, including wild felids. like domestic cats, wild felids are involved in the complete infective cycle of t. gondii, as they can host in their gastrointestinal tract sexually mature parasites and shed infective oocysts in their feces. in order to evaluate the importance of this wildlife reservoir, 438 serum samples collected between 1984 and 1999 from 438 pumas (felis concolor) and from 58 bobcats (lynx rufus) from north ...200415019138
seroprevalence of bartonella infection in american free-ranging and captive pumas (felis concolor) and bobcats (lynx rufus).bartonella henselae is the main agent of cat scratch disease in humans and domestic cats are the main reservoir of this bacterium. we conducted a serosurvey to investigate the role of american wild felids as a potential reservoir of bartonella species. a total of 479 samples (439 serum samples and 40 nobuto strips) collected between 1984 and 1999 from pumas (felis concolor) and 91 samples (58 serum samples and 33 nobuto strips) collected from bobcats (lynx rufus) in north america, central americ ...200415099499
puma lentivirus is controlled in domestic cats after mucosal exposure in the absence of conventional indicators of immunity.a high percentage of free-ranging pumas (felis concolor) are infected with feline lentiviruses (puma lentivirus, feline immunodeficiency virus pco [fiv-pco], referred to here as plv) without evidence of disease. plv establishes productive infection in domestic cats following parenteral exposure but, in contrast to domestic cat fiv, it does not cause t-cell dysregulation. here we report that cats exposed to plv oro-nasally became infected yet rapidly cleared peripheral blood mononuclear cell (pbm ...200515708998
development and validation of puma (felis concolor) cytokine and lentivirus real-time pcr detection systems.studies of immune correlates of disease outcome associate humoral immune response mediated by t-helper 2 cytokines (il-4, il-10) with more virulent disease relative to a cell-mediated response driven by t-helper 1 cytokines (il-2, ifn-gamma), particularly in viral and other intra-cellular infections. specifically, the kinetics of both human immunodeficiency virus (hiv) and feline immunodeficiency virus (fiv) infection are closely associated with type 1 versus type 2 cytokine profiles. puma (feli ...200515734541
phylogenetic studies of pantherine cats (felidae) based on multiple genes, with novel application of nuclear beta-fibrinogen intron 7 to carnivores.the pantherine lineage of the cat family felidae (order: carnivora) includes five big cats of genus panthera and a great many midsized cats known worldwide. presumably because of their recent and rapid radiation, the evolutionary relationship among pantherines remains ambiguous. we provide an independent assessment of the evolutionary history of pantherine lineage using two complete mitochondrial (mt) genes (nd2 and nd4) and the nuclear beta-fibrinogen intron 7 gene, whose utility in carnivoran ...200515804417
seroprevalence and genomic divergence of circulating strains of feline immunodeficiency virus among felidae and hyaenidae species.feline immunodeficiency virus (fiv) infects numerous wild and domestic feline species and is closely related to human immunodeficiency virus (hiv) and simian immunodeficiency virus (siv). species-specific strains of fiv have been described for domestic cat (felis catus), puma (puma concolor), lion (panthera leo), leopard (panthera pardus), and pallas' cat (otocolobus manul). here, we employ a three-antigen western blot screening (domestic cat, puma, and lion fiv antigens) and pcr analysis to sur ...200515956574
ticks (acari: ixodida) on wild carnivores in brazil.the present study reports field data of ticks infesting wild carnivores captured from july 1998 to september 2004 in brazil. additional data were obtained from one tick collection and from previous published data of ticks on carnivores in brazil. during field work, a total of 3437 ticks were collected from 89 cerdocyon thous (crab-eating fox), 58 chrysocyon brachyurus (maned wolf), 30 puma concolor (puma), 26 panthera onca (jaguar), 12 procyon cancrivorus (crab-eating raccoon), 4 speothos venati ...200516082932
the rise and fall of psoroptic scabies in bighorn sheep in the san andres mountains, new mexico.between 1978 and 1997, a combination of psoroptic scabies (psoroptes spp.), mountain lion (puma concolor) predation, and periodic drought reduced a population of native desert bighorn sheep (ovis canadensis) in the san andres mountains (sam), new mexico, from >200 individuals to a single ewe. in 1999, this ewe was captured, ensured to be psoroptes-free, and released back into the sam. eleven radio-collared rams were translocated from the red rock wildlife area (rrwa) in new mexico into the sam r ...200516244062
potential existence of a sylvatic cycle of taenia ovis krabbei in patagonia, deer (cervus elaphus) were introduced in southern latin america about a century ago and characteristics of the invasion raise concerns over their epidemiological role for various diseases. we report on the possible occurrence of taenia ovis krabbei established in a sylvatic cycle in patagonia. hook characters, size, appearance, and location of a cysticercus from a wild red deer are consistent with taenia ovis ovis or t. o. krabbei. although it is not possible to differentiate between t. o. o ...200616289565
feline immunodeficiency virus and puma lentivirus in florida panthers (puma concolor coryi): epidemiology and diagnostic issues.this study documents the seroprevalence of feline immunodeficiency virus (fiv) and puma lentivirus (plv) in free-ranging and captive florida panthers (puma concolor coryi) (n = 51) and translocated texas cougars (p. concolor stanleyana) (n = 10) from 1985 to 1998. the sera were tested for anti-fiv antibodies by enzyme-linked immunosorbent assay (elisa) and western blot tests. the elisas were read kinetically (kela) and the sera were retrospectively examined by plv peptide elisa. eleven panthers ...200616437306
a virus reveals population structure and recent demographic history of its carnivore host.directly transmitted parasites often provide substantial information about the temporal and spatial characteristics of host-to-host contact. here, we demonstrate that a fast-evolving virus (feline immunodeficiency virus, fiv) can reveal details of the contemporary population structure and recent demographic history of its natural wildlife host (puma concolor) that were not apparent from host genetic data and would be impossible to obtain by other means. we suggest that rapidly evolving pathogens ...200616439664
ectoparasites of free-ranging pumas and jaguars in the paraguayan chaco.ectoparasites were collected from seven puma (puma concolor) and seven jaguar (panthera onca) live-captures (each representing six different animals) in the paraguayan chaco from 2002 to 2004. the same five species of ectoparasites were recovered from both host species: the flea, pulex simulans (total on both hosts combined=30 male, 49 female), and the ticks, amblyomma cajennense (1 male, 4 female, 46 nymphs, 241 larvae), amblyomma parvum (42 male, 25 female), amblyomma tigrinum (1 male, 7 femal ...200616464533
feline lentivirus evolution in cross-species infection reveals extensive g-to-a mutation and selection on key residues in the viral polymerase.factors that restrict a virus from establishing productive infection in a new host species are important to understand because cross-species transmission events are often associated with emergent viral diseases. to determine the evolutionary pressures on viruses in new host species, we evaluated the molecular evolution of a feline immunodeficiency virus derived from a wild cougar, puma concolor, during infection of domestic cats. analyses were based on the coding portion of genome sequences reco ...200616501082
listeric meningoencephalomyelitis in a cougar (felis concolor): characterization by histopathologic, immunohistochemical, and molecular methods.listeria monocytogenes has been recognized as an important food-borne pathogen in animals. records of the disease caused by this bacterium in large felids are, however, rare. the nervous form of listeriosis was diagnosed in a 12-year-old male cougar (felis concolor) with a several-day history of neurologic disease characterized by excess salivation, head pressing, and circling that progressed to recumbency and death. microscopically, the main alteration in the brain and spinal cord was a variabl ...200616672590
isolation of malassezia spp. from cerumen of wild felids.the objective of this study was to determine the presence of different species of the genus malassezia in the healthy external auditory canal of wild felids maintained in captivity. one hundred and thirty-two adult animals (264 samples of cerumen), 77 males (58.3%) and 55 females (41.7%), were studied: large felids (55 animals) - 26 lions (panthera leo), 13 tigers (panthera tigris), 6 leopards (panthera pardus), 6 jaguars (panthera onca), 2 cheetahs (acinonyx jubatus), 2 pumas (puma concolor); s ...200616772235
t-lymphocyte profiles in fiv-infected wild lions and pumas reveal cd4 depletion.feline immunodeficiency virus (fiv) is a lentivirus related to human immunodeficiency virus (hiv) that causes feline aids in the domestic cat (felis catus). serological surveys indicate that at least 25 other species of cat possess antibodies that cross-react with domestic cat fiv. most infected nondomestic cat species are without major symptoms of disease. long-term studies of fiv genome variation and pathogenesis reveal patterns consistent with coadaptation of virus and host in free-ranging fi ...200616870846
molecular detection and characterization of cytauxzoon felis and a babesia species in cougars from florida.piroplasms, morphologically indistinguishable from cytauxzoon felis, previously were detected in 36% of cougars in florida. we utilized a nested 18s rrna assay, which amplifies dna from all piroplasms, to screen blood samples collected from 41 cougars from florida (39 native florida panthers [puma concolor coryi] and two translocated texas cougars [p. c. stanleyana]) from 1989-2005. thirty-nine of the 41 cougars (95%) were positive for piroplasms; however, sequence analysis and restriction enzym ...200616870859
gastrointestinal helminths of free-ranging florida panthers (puma concolor coryi) and the efficacy of the current anthelmintic treatment protocol.thirty-five florida panthers (puma concolor coryi [bangs, 1899]) collected from six counties in southern florida between 1978 and 2003 were examined at necropsy for gastrointestinal helminths. the panthers were placed into two groups: 1) treated with anthelmintics (n = 17), and 2) untreated (n = 18). nine species of helminths (one trematode, six nematodes, and two cestodes) were identified in the untreated panthers. the most prevalent helminths were alaria marcianae (larue, 1917) (100%), spirome ...200616870865
first evidence of feline herpesvirus, calicivirus, parvovirus, and ehrlichia exposure in brazilian free-ranging felids.serum samples from 18 pumas (puma concolor), one ocelot (leopardus pardalis), and two little spotted cats (leopardus tigrinus) collected from free-ranging animals in brazil between 1998 and 2004 were tested by indirect immunofluorescence (ifa) for antibodies to feline herpesvirus 1 (fhv 1), calicivirus (fcv), coronavirus (fcov), parvo-virus (fpv), ehrlichia canis, anaplasma pha-gocytophilum, and bartonella henselae. serum samples also were tested, by western blot and elisa, for feline leukemia v ...200616870878
hepatic yersiniosis in a cougar (felis concolor).a cougar (felis concolor) was diagnosed with hepatic yersiniosis by bacterial culture and histopathology. the animal had a 2-week history of anorexia and jaundice before its death. grossly, the liver exhibited caseo-necrotic foci. histopathologically, there was necrotizing and suppurative hepatitis, with large numbers of intralesional gram-negative coccobacilli. additional hepatic lesions included central vein thrombosis, lymphoplasmacytic portal hepatitis, and capsulitis. yersinia pseudotubercu ...200617037628
factors associated with pathogen seroprevalence and infection in rocky mountain cougars.serological and genetic material collected over 15 years (1990-2004) from 207 cougars (puma concolor) in four populations in the rocky mountains were examined for evidence of current or prior exposure to feline immunodeficiency virus (fiv), feline parvovirus (fpv), feline coronavirus (fcov), feline calicivirus (fcv), canine distemper virus (cdv), feline herpesvirus (fhv), and yersinia pestis. serologic data were analyzed for annual variation in seroconversions to assess whether these pathogens a ...200617092891
risk factors associated with sero-positivity to toxoplasma gondii in captive neotropical felids from brazil.from september 1995 to february 2001, blood samples were collected from 865 neotropical felids belonging to 8 different species. these animals were housed in 86 institutions located in 78 cities of 20 brazilian states. our goal was to identify the risk factors associated with sero-positivity to toxoplasma gondii in captive neotropical felids from brazil. all serum samples were tested by the modified agglutination test (mat), using formalin-fixed whole tachyzoites and mercaptoethanol. for each an ...200717140683
genetic consequences of sex-biased dispersal in a solitary carnivore: yellowstone cougars.male-biased dispersal is a common trait in mammals, including carnivores, but its genetic consequences at the population level have been rarely considered for solitary species. we used long-term genetic data from cougars (puma concolor) in and around yellowstone national park to test predictions based on differences in dispersal behaviour among males and females. consistent with frequent long-distance dispersal of males, we found support for our prediction of less than expected allele sharing in ...200617148391
neutralizing antibodies against feline herpesvirus type 1 in captive wild felids of brazil.feline herpesvirus type 1 infection affects domestic cats, causing mainly upper respiratory tract diseases. although this infection has been described in captive and free-ranging wild felids from europe, asia, north america, and africa, no information is available on its occurrence among wild felids of brazil. in this study, 250 serum samples of six species of brazilian captive wild felids (leopardus tigrinus, leopardus wiedii, herpailurus yaguarondi, puma concolor, leopardus pardalis, and panth ...200517312763
ancient papillomavirus-host co-speciation in felidae.estimating evolutionary rates for slowly evolving viruses such as papillomaviruses (pvs) is not possible using fossil calibrations directly or sequences sampled over a time-scale of decades. an ability to correlate their divergence with a host species, however, can provide a means to estimate evolutionary rates for these viruses accurately. to determine whether such an approach is feasible, we sequenced complete feline pv genomes, previously available only for the domestic cat (felis domesticus, ...200717430578
measurement of thyroid hormones (thyroxine, t4; triiodothyronine, t3) in captive nondomestic felids.the aim of this research was to obtain basic values for the evaluation of thyroid function in nondomestic felids. serum thyroid hormone concentrations (thyroxine, t4; triiodothyronine, t3) were measured by radioimmunoassay in 145 cats, representing nine species of captive nondomestic felids: jaguar (panthera onca), n = 49; puma (puma concolor), n = 10; ocelot (leopardus pardalis), n = 22; oncilla (leopardus tigrinus), n = 12; geoffroy (oncifelis geoffroyi), n = 4; jaguarundi (herpailurus yaguaro ...200717469287
detection of toxolasma gondii in captive wild felids.toxoplasma gondii can infect all species of warm-blooded animals, including humans, and causes serious diseases in immunocompromized hosts. live tachyzoites derived from serial passage in hela culture were used in the sabin-feldman dye test for detection of toxoplasma gondii antibody in serum samples of 21 captive wild felids including one fishing cat (prion nailurus viverrina), one leopard (panthera pardus), two flat-headed cats (prion nailurus planiceps), 6 tigers (panthera tigris), two leopar ...200617547043
frequent transmission of immunodeficiency viruses among bobcats and pumas.with the exception of human immunodeficiency virus (hiv), which emerged in humans after cross-species transmissions of simian immunodeficiency viruses from nonhuman primates, immunodeficiency viruses of the family lentiviridae represent species-specific viruses that rarely cross species barriers to infect new hosts. among the felidae, numerous immunodeficiency-like lentiviruses have been documented, but only a few cross-species transmissions have been recorded, and these have not been perpetuate ...200717670835
laboratory findings in acute cytauxzoon felis infection in cougars (puma concolor couguar) in florida.intraerythrocytic piroplasms, morphologically indistinguishable from cytauxzoon felis, were identified in stained blood films from more than one third of free-ranging cougars (puma concolor couguar) in southern florida in a study that failed to demonstrate negative effects of piroplasm infection on measured hematologic parameters. however, a recent study with a nested 18s rrna polymerase chain reaction (pcr) assay identified only 9% of the free-ranging cougars in southern florida as infected wit ...200717679513
variability in assays used for detection of lentiviral infection in bobcats (lynx rufus), pumas (puma concolor), and ocelots (leopardus pardalis).although lentiviruses similar to feline immunodeficiency virus (fiv) are known to infect numerous felid species, the relative utility of assays used for detecting lentiviral infection has not been compared for many of these hosts. we tested bobcats (lynx rufus), pumas (felis concolor), and ocelots (leopardus pardalis) for exposure to lentivirus using five different assays: puma lentivirus (plv), african lion lentivirus (llv), and domestic cat fiv-based immunoblots, a commercially available enzym ...200717984266
relationships between human disturbance and wildlife land use in urban habitat fragments.habitat remnants in urbanized areas typically conserve biodiversity and serve the recreation and urban open-space needs of human populations. nevertheless, these goals can be in conflict if human activity negatively affects wildlife. hence, when considering habitat remnants as conservation refuges it is crucial to understand how human activities and land uses affect wildlife use of those and adjacent areas. we used tracking data (animal tracks and den or bed sites) on 10 animal species and infor ...200818254856
genetic characterization of feline leukemia virus from florida panthers.from 2002 through 2005, an outbreak of feline leukemia virus (felv) occurred in florida panthers (puma concolor coryi). clinical signs included lymphadenopathy, anemia, septicemia, and weight loss; 5 panthers died. not associated with felv outcome were the genetic heritage of the panthers (pure florida vs. texas/florida crosses) and co-infection with feline immunodeficiency virus. genetic analysis of panther felv, designated felv-pco, determined that the outbreak likely came from 1 cross-species ...200818258118
the molecular biology and evolution of feline immunodeficiency viruses of cougars.feline immunodeficiency virus (fiv) is a lentivirus that has been identified in many members of the family felidae but domestic cats are the only fiv host in which infection results in disease. we studied fivpco infection of cougars (puma concolor) as a model for asymptomatic lentivirus infections to understand the mechanisms of host-virus coexistence. several natural cougar populations were evaluated to determine if there are any consequences of fivpco infection on cougar fecundity, survival, o ...200818295904
isolation and genetic characterization of toxoplasma gondii from raccoons (procyon lotor), cats (felis domesticus), striped skunk (mephitis mephitis), black bear (ursus americanus), and cougar (puma concolor) from canada.viable toxoplasma gondii was isolated by bioassay in mice from tissues of 2 feral cats (felis domesticus), 2 raccoons (procyon lotor), a skunk (mephitis mephitis) trapped in remote locations in manitoba, canada, and a black bear (ursus americanus) from kuujjuaq, northern quebec, canada. genotyping of these t. gondii isolates using polymorphisms at 10 nuclear markers including sagi, sag2, sag3, btub, gra6, c22-8, c29-2, l358, pk1, and an apicoplast marker apico revealed 4 genotypes. none of the i ...200818372620
[seasonal evaluation of mammal species richness and abundance in the "mário viana" municipal reserve, mato grosso, brasil].we evaluated seasonal species presence and richness, and abundance of medium and large sized mammalian terrestrial fauna in the "mário viana" municipal biological reserve, nova xavantina, mato grosso, brazil. during 2001, two monthly visits were made to an established transect, 2,820 m in length. records of 22 mammal species were obtained and individual footprint sequences quantified for seasonal calculation of species richness and relative abundance index (x footprints/km traveled). all 22 spec ...200618491629
seroepidemiology of toxoplasma gondii in zoo animals in selected zoos in the midwestern united states.toxoplasma gondii infections in zoo animals are of interest because many captive animals die of clinical toxoplasmosis and because of the potential risk of exposure of children and elderly to t. gondii oocysts excreted by cats in the zoos. seroprevalence of t. gondii antibodies in wild zoo felids, highly susceptible zoo species, and feral cats from 8 zoos of the midwestern united states was determined by using the modified agglutination test (mat). a titer of 1:25 was considered indicative of t. ...200818605803
molecular evidence for a novel encapsulated genotype of trichinella from patagonia, present, trichinella spiralis is the only species of this genus reported from south america. herein, we detail a molecular analysis of a new encapsulated isolate of muscle larvae of trichinella, found in a mountain lion (puma concolor) coming from the patagonia, argentina. we studied three dna regions previously probed to be useful for the identification of all eleven recognized trichinella genotypes: expansion segment 5 (es5), cytochrome c-oxidase subunit i (coi) and 5s ribosomal dna interge ...200818650017
epizootiology and management of feline leukemia virus in the florida puma.feline leukemia virus (felv) was not detected in florida pumas (puma concolor coryi) in almost 20 yr of surveillance; however, the finding of two felv antigen-positive pumas during the 2002-2003 capture season led to an investigation of felv in the population. between january 1990 and april 2007, the proportion of pumas testing felv antibody positive increased, with antibody-positive pumas concentrated in the northern portion of puma range. five of 131 (4%) pumas sampled between july 2000 and ap ...200818689639
[felines: an alternative in genetic toxicology studies?].the micronuclei (mn) test carry out in peripheral blood is fast, simple, economic and it is used to detect genotoxic environmental agents. mn are fragments of chromosomes or complete chromosomes remaining in the cytoplasm after cell division, which increase when organisms are exposed to genotoxic agents. therefore, species with the highest values of spontaneous micronucleated erythrocytes (mne) are the most suitable to be potentials biomonitor of micronucleogenic agents, using a drop of blood. n ...200819256458
first evidence of canine distemper in brazilian free-ranging felids.serum samples from 19 jaguars (panthera onca), nine pumas (puma concolor), and two ocelots (leopardus pardalis) were collected between january 1999 and march of 2005 and tested for presence of canine distemper virus (cdv). all cats were free-ranging animals living in two protected areas in the brazilian atlantic forest. in addition, 111 domestic dogs from nearby areas were sampled for cdv. our results show the first evidence of cdv exposure in brazilian free-ranging felids. from the 30 samples a ...200919259737
[occurrence of platynosomum illiciens in enclosured wild cats in the state of bahia, brazil].this paper reports the occurrence of eggs of platynosomum illiciens, found in the feces of three species of wild cats herpailurus yagouaroundi, puma concolor and leopardus tigrinus from material collected at the enclosures of the park zoobotânico getúlio vargas in salvador, bahia, brazil. it is the first record of this parasite in p. onca and l. tigrinus.200819265585
leishmania(leishmania) chagasi in captive wild felids in brazil.this study used a pcr-rflp test to determine the presence of leishmania (leishmania) chagasi in 16 captive wild felids [seven puma concolor (linnaeus, 1771); five panthera onca (linnaeus, 1758) and four leopardus pardalis (linnaeus, 1758)] at the zoological park of the federal university of mato grosso, brazil. amplification of leishmania spp. dna was seen in samples from five pumas and one jaguar, and the species was characterized as l. chagasi using restriction enzymes. it is already known tha ...201019740501
capillaria hepatica in puma concolor: first report in brazil.capillaria hepatica was detected by histopathologic diagnosis in two cougars that were shot in april 2008 in painel, santa catarina, brazil. macroscopic analysis of their livers revealed the presence of diffuse granulomas, and the histopathologic analysis indicated the presence of c. hepatica eggs, surrounded by mononuclear inflammatory cell infiltrate, small foci of necrosis, and mild-to-moderate fibrosis. this is the first report of c. hepatica in cougars (puma concolor) in brazil.200919746880
mountain lions prey selectively on prion-infected mule deer.the possibility that predators choose prey selectively based on age or condition has been suggested but rarely tested. we examined whether mountain lions (puma concolor) selectively prey upon mule deer (odocoileus hemionus) infected with chronic wasting disease, a prion disease. we located kill sites of mountain lions in the northern front range of colorado, usa, and compared disease prevalence among lion-killed adult (> or =2 years old) deer with prevalence among sympatric deer taken by hunters ...201019864271
restrictions to cross-species transmission of lentiviral infection gleaned from studies of fiv.more than 40 species of primates and over 20 species of cats harbor antibodies that sero-react to lentiviral antigens. in nearly all cases where viral genetic analysis has been conducted, each host species is infected with a unique lentivirus. though lentivirus clades within a species can be substantially divergent, they are typically monophyletic within that species. a notable significant departure from this observation is apparent cross-species transmission of fiv between bobcats (lynx rufus) ...201019896218
molecular epidemiology of feline immunodeficiency virus in the domestic cat (felis catus).studying the evolutionary mechanisms of feline immunodeficiency virus in the domestic cat (felis catus), fiv(fca), provides a good comparison to other lentiviruses, such as hiv and fiv(pco) in the cougar (puma concolor). we review the current epidemiological and evolutionary findings of fiv(fca). in addition to the five accepted fiv(fca), subtypes, several recent phylogenetic studies have found strains that form separate clades, indicative of novel subtypes. in new zealand cats, these strains of ...201019896220
the role of landowners in jaguar conservation in sonora, mexico.the northernmost known breeding population of jaguars occurs in the municipality of nácori chico, sonora, mexico about 270 km from the united states-mexico border and may be the source from which jaguars sighted in the united states dispersed. since 1999 at least 11 jaguars (panthera onca) had been illegally killed in the area due to predator control programs. we initiated a jaguar landowner-based conservation plan in 2004. the eight participating landowners agreed to suspend predator control pr ...201020136872
trichinella infection in wild animals from endemic regions of argentina.natural infection with trichinella has been described in more than 150 mammalian species. however, few reports of trichinella infection in wild animals have come from argentina. in this study, muscle tissue was obtained from wild animals in argentina with the aim of evaluating the presence of trichinella. a total of 169 muscle samples were collected to determine the presence of trichinella larvae by artificial digestion. the 169 muscle samples originated from 12 species including 36 opossums (di ...201020424859
temporal autocorrelation functions for movement rates from global positioning system radiotelemetry data.autocorrelation has been viewed as a problem in telemetry studies because sequential observations are not independent in time or space, therefore violating assumptions for statistical inference. yet nearly all ecological and behavioural data are autocorrelated in both space and time. we argue that there is much to learn about the structure of ecological and behavioural data from patterns of autocorrelation. such patterns include periodicity in movement and patchiness in spatial data, which can b ...201020566498
mosaics of exotic forest plantations and native forests as habitat of pumas.there is a general lack of information on the impact of forest plantations and the presence of urban settlements on populations of resource-demanding species such as large felids. to partially address this problem, a project study was conducted to find out whether mosaics of forest plantations and native vegetation can function as an adequate habitat for pumas (puma concolor) in southern brazil. the study was conducted within a 1255-km(2) area, managed for planted stands of pinus spp. and eucaly ...201020665214
molecular and serologic detection of ehrlichia spp. in endangered brazilian wild captive felids.ehrlichiosis, an emergent tick-borne disease that affects both humans and animals, may represent a threat to the survival and preservation of wild felids in brazil. there are few studies of ehrlichiosis in wild felids in brazil, but ehrlichia spp. are present in domestic cats. antibodies to ehrlichia canis have been reported in a puma (puma concolor). in this study we assessed the presence of these hemoparasites in the blood of brazilian wild captive felids. of the 72 animals tested, 5 (7%) were ...201020688716
distribution and prevalence of cytauxzoon felis in bobcats (lynx rufus), the natural reservoir, and other wild felids in thirteen states.cytauxzoon felis, a protozoan parasite of wild and domestic felids, is the causative agent of cytauxzoonosis in domestic and some exotic felids in the united states. the bobcat (lynx rufus) is the natural reservoir for this parasite, but other felids such as florida panthers (puma concolor coryii) and domestic cats may maintain long-term parasitemias and serve as reservoirs. experimentally, two tick species, dermacentor variabilis and amblyomma americanum, have demonstrated the ability to transm ...201021071149
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