search for epizootic-like venezuelan encephalitis virus at enzootic habitats in guatemala during 1969-1971.seventy-four strains of venezuelan encephalitis (ve) virus recovered from sentinel hamsters or mosquitoes at enzootic habitats in guatemala in the two years following the 1969 epidemic-equine epizootic were examined for ability to produce small plaques in vero african green monkey kidney cell cultures, like isolates obtained during the epizootic. (a) one strain recovered from a sentinel hamster in late october 1969 at an enzootic habitat near the epicenter of the hemagglutination-inhibition (hi) ...19767138
characteristics of poliovirus type 3 strains after passage through monkey central nervous system.cercopithecus aethiops monkeys were inoculated intracerebrally with sabin's type 3 polivoirus strain, and with strains isolated from subjects vaccinated with leon 12a1b and from their contacts. sample of brain, spinal cord, kidneys, lymph nodes, blood and spinal fluid were taken. from these materials, (except of kidneys) 23 poliovirus strains of type 3 were isolated. genetic markers rct 40.3, rct 41, d and ist were determined for these strains. the changes observed in genetic markers of strains ...197715438
role of normal cell antigens in the establishment of measles virus in newborn white mice.the edmontson strain of measles virus was adapted to the brains of newborn white mice to which extracts from african green monkey kidney (vero) cells used for maintenance of measles virus had been injected intracerebrally. the animals showed symptoms of involvement of the central nervous system (pareses, paralyses) which reappeared in subsequent passages.197717289
cv-1 monkey kidney cell line -- a highly susceptible substrate for diagnosis and study of arboviruses.the cv-1 cell line, derived from cercopithecus aethiops monkey kidneys, acquired in the course of serial passaging an epitheloid morphology and heteroploid karyotype. as compared with vero and gmk cell lines, the cv-1 line proved to be more susceptible to a broad spectrum of arboviruses belonging to the families togaviridae and bunyaviridae. most of them reached high titres and were markedly cytopathic in cv-1 cells so that all tests based on the cytopathic effect could be carried out. the cv-1 ...197826197
characterization of a virus isolated from a case of human infectious hepatitis.a new viral agent, isolated from the serum of an infectious hepatitis patient and designated as agent ii-b, was extensively studied in in vitro and in vivo systems. agent ii-b multiplied well in primary and serial animal cell cultures and in embryonated hen's eggs. quantal and quantitative infectivity assays were performed in monolayers of african green monkey kidney cells. effective concentrations of 5-iodo-2-deoxyuridine and guanidine hydrochloride did not inhibit the multiplication of agent i ...197936750
the effect of oral pancreatic extract on jejunal bactericidal activity in protein-deficient vervet monkeys challenged with vibrio cholerae.eleven vervet monkeys (cercopithecus aethiops) were fed with an "o" protein diet. after the serum albumin level fell below 2.5 g/100 ml the animals and 4 controls, which received regular monkey chow, were orally infected with a monkey-adapted strain of vibrio cholerae. the total bactericidal activity of the jejunal fluid decreased during feeding with "o" protein diet, but increased after challenge with v. cholerae in all groups. the non-immunoglobulin-bound bactericidal activity, which also decr ...197941422
development and utilization of complement-fixation and immune adherence tests for human hepatitis a virus and antibody.the reliable propagation of cr326 strain of human hepatitis a virus in saguinus mystax marmosets has permitted the development of specific serum neutralization, complement-fixation (cf), and immune adherence (ia) assays for hepatitis a antigen and antibody. the cf and ia assay were made possible by the use of livers of cr326-infected marmosets as a source of hepatitis a antigen. all assays were shown to be specific for hepatitis a. patients with hepatitis b did not show development of hepatitis ...197553013
safety of viral vaccine cell substrates: a reevaluation.primary cultures of african green monkey kidney and rabbit kidney as well as diploid cell lines wi-38 and dbs-frhl-2 were examined for evidence of tumorigenicity and latent rna tumor viruses. cells inoculated into immunosuppressed newborn hamsters and rhesus monkeys were not tumorigenic. cells treated with 2'-deoxy-5-iodouridine to induce the production of latent viruses were examined by electron microscopy, density gradient centrifugation, and the reverse transcriptase enzyme assay. no evidence ...197663564
influence of semipurified diets on atherosclerosis in african green monkeys. 197764369
[chronic infection caused by the simian mason-pfizer virus in primate cell cultures].cell cultures of monkey prepuce (rhfs) and african green monkey kidney (bsc-1) were infected once with simian mason-pfizer virus (mpv) and virus expression in the course of establishment of chronic infection was studied. the productive infection was characterized by changes in the cell metabolism (dna synthesis increased 2-3-fold as early as the "zero" passage), the appearance of gs-antigen, formation of virions of type d and high activity of rna-dependent dna-polymerase. multinuclear giant cell ...197878587
modulation by cell trypsinization of sendai virus expression in african green monkey kidney cells: first infection and establishment of a carrier state.two african green monkey kidney (agmk) cell lines, 37rc (interferon-producing) and vero (non-interferon-producing), were infected by egg-grown sendai virus passaged in eggs at high and low m.o.i. the appearance of haem-adsorption, and cytopathic effect (c.p.e.) as well as the presence of haemagglutinating virions in the supernates were much more pronounced with a virus seed obtained with 10(-3) diluted passages than with a seed obtained with undiluted inoculum. they were also independent of inte ...197994347
simian rotavirus sa-11 plaque formation in the presence of trypsin.incorporation of 5 micrograms of trpsin per ml of the overlay (eagle minimal essential medium-0.7% ionagar no. 2) was found to be necessary for plaque formation by simian rotavirus sa-11. plaques of 3 to 4 mm in diameter were produced in ma-104 cells after 5 days of incubation at 37 degrees c. plaque size was even larger (5 to 6 mm) in monolayers of african green monkey kidney cells. addition of diethyl-aminotheyl-dextran, protamine sulfate, or 5-bromodeoxyuridine to the trypsin-containing overl ...197994597
actin filaments in trabecular endothelial cells in eyes of the vervet monkey. (cercopithecus aethiops).an electron microscopic study was performed on the trabecular meshwork of two monkeys (cercopithecus aethiops). specimens from one animal revealed numerous cytoplasmic filaments of 45-65 a thickness in endothelial cells both on the trabeculae in the wall of schlemm's canal after conventional glutaraldehyde and osmium fixation. using a histochemical technique it was shown in specimens from the other animal that these filaments consist of actin material. this evidence of intracellular actin filame ...197896655
circadian rhythm of cholesterol-7alpha-hydroxylase and cortisol in the african green monkey (cercopithecus aethiops).cholesterol-7alpha-hydroxylase in african green monkey liver had an apparent km of 1.65-10(-4) m cholesterol and a ph optimum of 7.4. the amplitudes of the circadian maxima of enzyme activity and serum cortisol levels were significantly greater in vervets than in grivets. fluctuations in enzyme activity and cortisol levels during the circadian cycle were positively correlated (r = 0.89). enzyme activities and hormone levels were 2.7-fold lower over a 24-h period in the grivet than in the vervet. ...197896863
experimental infection of the genital tract of female grivet monkeys by mycoplasma hominis.mycoplasma hominis, a common inhabitant of the mucosae of the genitourinary tract of human and nonhuman primates, was inoculated directly into the uterine tubes of five laparotomized grivet monkeys. a self-limiting acute salpingitis and parametritis developed within a few days in all animals. although there were no clinical signs of overt disease, the gross pathology was characterized by pronounced oedematous swelling and hyperaemia of the tubes and parametria. microscopically, cellular infiltra ...197897224
immunochemical studies on the 5-methylcytosine content of african green monkey satellite dna. 197898282
periodontal and pulpal healing of monkey incisors preserved in tissue culture before replantation.the effect of delayed replantation and tissue culture as a prevention against root resorption was examined in green vervet monkeys (cercopithecus aethiops). extracted incisors were kept in tissue culture medium (eagle's medium) for 5--14 days before replantation. the extra-alveolar dry period before tissue culture ranged from to 0 to 60 min. incisors not subjected to tissue culture served as controls. the animals were sacrificed 8 weeks after replantation. the following histologic parameters wer ...197898456
an experimental study of the central gustatory pathways in the monkey, macaca mulatta and cercopithecus aethiops.1. the cortex at the base of the central fissure, that is the fronto-parietal operculum, represents primary sensory receptive cortex for gustatory modalities. 2. this primary receptive cortex for taste is linked to the anterior island of reil by short inter- and intracortical association fibers. thus the anterior island is a gustatory association area involved in the subjective recognition of gustatory modalities. 3. the nucleus ventralis posteromedialis pars parvocellularis of the dorsal thalam ...197898582
pathology of bolivian hemorrhagic fever in the african green monkey.gross and microscopic pathological findings are presented for an african green monkey model of fatal bolivian hemorrhagic fever. six animals were inoculated with 1,000 plaque-forming units of machupo virus, the etiological agent of bolivian hemorrhagic fever. five of the monkeys died within 13 days with signs of fever, anorexia, shock, and hemorrhage. the sixth monkey survived until the 24th day and died with signs of central nervous system disease. gross lesions in the five monkeys that die in ...197899059
rosette formation by human lymphoid cells with monkey red blood cells.the present paper describes a study on rosette formation by human lymphoid cells with monkey erythrocytes (mrbc) obtained from two species: african green monkey (cercopithecus aethiops) and rhesus monkey (macaca mulata). all the lymphocyte preparations obtained from peripheral blood, tonsils, thymocytes, bone-marrow and spleen biopsies formed rosettes in variable proportions with erythrocytes obtained from these two monkey species tested. cells of established lymphoblastoid cell lines characteri ...1978100044
the diet and feeding behaviour of cercopithecus aethiops tantalus.the feeding behaviour of representative groups of tantalus monkeys (cercopithecus aethiops tantalus) was observed during 1974-1975 in three habitats in cameroon. observations took place during the wet and dry seasons in sahelian savannah, guinea savannah and farmed forest. the tantalus monkeys were similar to other races of c.aethiops that have been studied in that they were omnivorous and lacked any narrow specialisations of either diet or feeding techniques. the dietary range was similar in al ...1978101428
variations in the blood clotting time and lipid patterns of the nigerian monkey, cercopithecus aethiops tantalus, during captivity.the blood clotting time and serum lipid values of newly-captured nigerian monkeys were determined during the period of adaptation. large fluctuations in serum free and total cholesterol and phospholipid values were observed throughout the 12-week period of investigation. the variations in blood clotting time were not significant (p greater than 0.05). these wide variations in lipid values suggest the need for caution in interpreting data from newly-captured subhuman primates.1978104089
implications for adult roles from differential styles of mother-infant bonding: an ethological study.ethological observations of maternal and infant behaviors of nine vervet monkey pairs (cercopithecus aethiops sabaeus) showed the effects of differential styles of early maternal responsiveness on later infant competence. those infants receiving the least amount of maternal responsiveness and the most time-off the mother in the first 3 months of development were more socially competent at 6 months of age. the results are discussed within current ethological "attachment" theories. the detachment ...1979105083
developmental staging and thalidomide teratogenicity in the green monkey (cercopithecus aethiops).the developmental stages of 11 cercopithecus aethiops embryos, 24 to 45 days post-mating, are described. the onset of organogenesis in this species is approximately five to seven days later than that reported for macaques and baboons. this temporal difference in the embryonic period is an important factor in the analysis of the subsequent teratogenic study. oral administration of thalidomide in single or multiple (3-day) treatment periods to pregnant green monkeys between days 28 and 33 resulted ...1978105418
resistance of african green monkey kidney cell lines to actinomycin d: drug uptake in 37 rc cells after persistent inhibition of transcription.37 rc cells, a cultured line derived from african green monkey kidneys, survived long treatments with actinomycin d (amd; 0.1 to 0.5 mug/ml) under strong inhibition of ribonucleic acid synthesis and blocking of cell division. one aspect of the complex cellular response to this treatment was a progressive lowering of the influx rate of amd and, consequently, of its endocellular concentration, leading to a late resurgence of transcription. overall protein synthesis decreased in amd-treated cells, ...1979106777
the pathology of untreated and antibiotic-treated experimental tularaemia in monkeys.grivet monkeys were infected intranasally with the virulent schu-s4 strain of f. tularensis. one group of animals remained untreated and two other groups received a 7-day course of kanamycin therapy starting on either the third or fourth day after infection. untreated monkeys developed pyrexia and mucopurulent oculonasal discharge and died 5--7 days after infection. all had pyogranulomatous lesions in the liver, spleen, respiratory tract and lymph nodes. electron microscopy of liver and spleen s ...1978106869
changes in whole blood and serum components of grivet monkeys with experimental respiratory francisella tularensis infection.grivet monkeys infected with virulent francisella tularensis strain schu s4 showed significant early changes in serum levels of trace metals, triglycerides and activities of alkaline phosphatase, lactate dehydrogenase and alpha-hydroxybutyrate dehydrogenase. free amino acid levels decreased slightly and there was a marked increase in the phenylalanine: tyrosine ratio. serum lysozyme activity and seromucoid levels also increased. kanamycin therapy produced remission of overt signs but the changes ...1978106870
isolation and preliminary characterization of the small circular dna present in african green monkey kidney (bsc-1) cells. 1978107539
experimental acute salpingitis in grivet monkeys provoked by chlamydia trachomatis.chlamydia trachomatis is a common cause of sexually transmitted diseases. recently it has been shown that chlamydiae are also responsible for complications to such lower genital tract infections. in this study, isolates of c. trachomatis from the fallopian tubes of patients with acute salpingitis were inoculated direct into the fallopian tubes of two, and through the cervical canal into the uterine cavity of one grivet monkey. the experimental infections resulted in a self-limited acute salpingi ...1979107723
bacterial myocarditis in african green monkeys (cercopithecus aethiops). 1979108846
studies on the organization of the alpha-satellite dna from african green monkey cells using restriction nucleases and molecular cloning.alpha-satellite dna from african green monkey cells was analysed with restriction nucleases in some detail confirming and complementing our earlier results. with ecori and haeiii (or bsuri isoschizomer), about 25 and 10%, respectively, of the satellite dna were cleaved into a series of fragments of the 172 bp repeat length and multiples thereof. to allow studies with fragments of homogeneous sequence unit length, hindiii fragments were covalently joined with the plasmid pbr 313. after transforma ...1979109356
schistosomiasis in omo national park of southwest ethiopia.schistosomiasis mansoni infection was found in more than 50 tourists who had visited omo national park, ethiopia, and bathed and swum in the mui river. a survey revealed schistosoma mansoni infection in 41% of park residents and in 33% of the neighboring suri people. eggs were found in stools and adult worms at autopsy of wild papio anubis and cercopithecus aethiops. trematode larvae were found in 27% of biomphalaria pfeifferi snails found in the mui river. the source of the disease and the impl ...1979110163
comparative normal levels of serum triiodothyronine and thyroxine in nonhuman primates.normative values were obtained for triiodothyronine and thyroxine from four species of old world primate (chimpanzees, rhesus monkeys, african green monkeys and talopoin monkeys) and a single species of new world primate (squirrel monkeys) represented by two subspecies, colombian and bolivian. the bolivian squirrel monkeys exhibited the lowest values for both triiodothyronine and thyroxine. male talapoins had the highest levels of thyroxine. significant differences were found in levels of triiod ...1979110976
the effect of oral pancreatic enzymes on the intestinal flora of protein-deficient vervet monkeys challenged with vibrio cholerae.jejunal bacterial flora in 11 protein deficient vervet monkeys (cercopithecus aethiops) and four controls was studied. these were the same animals from an investigation previously reported in which it was shown that pancreatic extract modified the course of cholera infection in protein-deficient monkeys. the present study reports in addition that these animals the fluid in the upper jejunum contained significantly increased numbers of bacteria including enterobacteriaceae compared to its prediet ...1979111538
normal values for some whole blood and serum components of grivet monkeys (cercopithecus aethiops).normal values for a number of blood components of grivet monkeys are reported. haematological data and values for glucose, cholesterol and urea are similar to those of rhesus monkeys. activities of alkaline phosphatase (1526 u/l), glutamine oxaloacetate transaminase (30.9 u/l), glutamine pyruvate transaminase (13.7 u/l), lactate dehydrogenase (629 u/l), alpha-hydroxybutyrate dehydrogenase (175 u/l), creatine phosphokinase (227 u/l), gamma-glutamyl transpeptidase (38.7 u/l) and sorbitol dehydroge ...1979113624
action of bacterial endotoxin and lipid a on mitochondrial enzyme activities of cells in culture and subcellular fractions.escherichia coli o127:b8 lipopolysaccharide (lps), prepared by the westphal procedure, caused a marked decrease in the activities of mitochondrial malate dehydrogenase, succinate dehydrogenase, and adenylate kinase in african green monkey kidney (vero) cells and primary cultures of mouse liver cells within 2 h after exposure to 10 micrograms of lps/ml of culture medium. these three enzyme activities leaked into the supernatant fraction, and cytochrome oxidase activity was lost from the mouse liv ...1979114491
early endothelial changes in experimental primate atherosclerosis.aortic tissues of cholesterol-fed african green monkeys were studied by light and electron microscopy. the sampled areas were either grossly normal, or showed minute grayish white translucent or yellowish white intimal elevations. samples of topographically corresponding areas from animals on normal diet served as controls. by light microscopy, the intimal elevations were either pure fatty dots or a combination of fatty dots and focal intimal edema. the most conspicuous ultrastructural findings ...1979114709
subunit structure of alpha-satellite dna containing chromatin from african green monkey cells.alpha-satellite dna containing chromatin from african green monkey cells (cv-1 cells) has been used to study the question whether or not nucleosomes are arranged in phase with the 172 bp repeat unit of the satellite dna. digestion experiments with dnaase ii led us to exclude a simple phase relationship between the nucleosomal and the satellite dna repeats. digestion of cv-1 nuclei with micrococcal nuclease and endogenous nuclease (s) produced a series of sharp bands in the satellite dna register ...1979114981
social isolation and other behavioral changes in groups of adult vervet monkeys (cercopithecus aethiops) produced by low, nonchronic doses of d-amphetamine.doses (0.1--0.7 mg/kg body weight) d-amphetamine were administered to three independent groups of green vervet monkeys (cerecopithecus aethiops). each group formed a triad with one male and two females. amphetamine changed both the individual and the social behavior patterns significantly. stereotypy and social isolation (withdrawal) were characteristic features of the amphetaminized animals. also stereotyped social grooming was observed. the results are discussed in relation to behavior changes ...1979116271
the repetitive sequence structure of component alpha dna and its relationship to the nucleosomes of the african green monkey. 1979117111
changes in the virus-host cell relationship in a stable non-virogenic cell line persistently infected with measles virus (bgm/mv).a non-virogenic african green monkey kidney cell line bgm/mv persistently infected with a neurotropic mouse brain-adapted strain of measles virus, was found to have undergone significant changes in the virus-host cell relationship between passages 35 and 119. rather than the stable non-cytopathic relationship previously reported in which approximately 100% of the cells contained measles antigens and less than 1% of the cells expressed cell surface measles antigen, we observed cyclic manifestatio ...1979118238
experimental infection of the genital tract of female grivet monkeys by mycoplasma hominis: effects of different routes of a previous study we produced acute salpingitis and parametritis in grivet monkeys by inoculation of mycoplasma hominis directly into the uterine tubes. with the purpose of examining in the same animal model the effect of more natural routes of infection, six female grivet monkeys were inoculated, two by two, with m. hominis by the following methods: (experiment a) into the uterine cavity through the cervical canal; (experiment b) into the uterine cavity by the same route, but after ligature o ...1979118928
long-term retention of a visually cued poison-based aversion in monkeys.retention of a poison-based aversion after a 3-yr. period by 8 vervet monkeys (cercopithecus aethiops) was found. the color of the visual cue appeared to effect the retention of the aversion.1979119208
reproductive and hormonal patterns in the african green monkey (cercopithecus aethiops).the results of breeding cercopithecus aethiops under time-mated laboratory conditions and analysis of total estrogen, progesterone, and lh concentrations in plasma during the menstrual cycle and plasma estrogen and progesterone concentrations during pregnancy indicate that this species is a suitable alternative for the rhesus monkey as a model for investigations of reproductive function in man.1979119860
effect of passage history on dengue-2 virus replication in subpopulations of human leukocytes.three passage levels of dengue-2 virus strain pr-159, obtained during the course of deriving the attenuated s-1 vaccine, were tested for their ability to replicate in subpopulations of human peripheral blood leukocytes: (i) 6th primary african green monkey kidney (pgmk) cell passage (parent virus); (ii) 19th pgmk cell passage of a small-plaque-forming clone derived from the parent virus (s-1 pgmk virus); and (iii) virus derived by four additional passages of the s-1 pgmk virus in diploid fetal r ...1979121109
[cytotoxic action of diisopropyl fluorophosphate (dfp) and bis-4-hydroximino-methyl-pyridine-1-methyl-(toxogonin) on kidney cultures of a monkey (cercopithecus aethiops) (author's transl)]. 1978121225
the fine structure of induced pulpitis in a monkey (cercopithecus aethiops). 1979121535
[demonstration by immunofluorescence of somatotropic cells and prolactin cells in the monkeys erythrocebus patas, cercopithecus aethiops and papio hamadryas].using antibodies against human sth and ovine prolactine, it is possible to identify somatotropic and prolactin cells in the pituitary anterior lobe of three species of monkeys: erythrocebus patas, cercopithecus aethiops and papio hamadryas. the somatotropic cells are numerous in both adult and infant male or female monkeys, especially in the lateral lobes of the pituitary gland. the prolactin cells can be distinguished in all pituitaries studied, regardless age or sex; indeed there are more nume ...1976140737
[demonstration by immunofluorescence of somatostatin cells in the islets of langerhans in 3 species of monkeys].indirect immunofluorescence technique with anti-somatostatin serum has allowed us to detect somatostatin containing cells in the langerhans' islets of three species of monkeys (macacus irus, papio hamadryas, cercopithecus aethiops). these cells are in close relationship with another cells, but occupy a more central position in the islets. thus, the distribution of somatostatin-containing cells in the monkey's endocrine pancreas has not a similar pattern as in rodent's pancreas; they have a compa ...1979160269
virus development in enucleate cells: echovirus, poliovirus, pseudorabies virus, reovirus, respiratory syncytial virus and semliki forest virus.a group of rna viruses, echovirus, poliovirus, reovirus, respiratory syncytial virus and semliki forest virus have been examined for ability to grow in enucleate african green monkey kidney (bsci) cells. semliki forest virus produced an almost normal yield of virus but poliovirus, echovirus, reovirus and respiratory syncytial virus, although showing clear evidence of virus replication when compared with a nuclear dna virus (pseudorabies virus) gave much lower yields than those from nucleate cell ...1975163289
stimulation of adenovirus replication in simian cells in the absence of a helper virus by pretreatment of the cells with iododeoxyuridine.pretreatment of african green monkey kidney cells with 50 mu g of 5'-iododeoxyruidine (iudr) per ml can modify their susceptibility to the replication of human adenovirus type 7 in the absence of simian virus 40 (sv40) although this enhancement of adenovirus replication is not as efficient as that of the helper sv40 virus. since the number of infectious centers remains unchanged after iudr pretreatment whereas the burst size of virus from each infected cell increases, the iudr appears to allow e ...1975163353
stimulation of non-histone chromosomal protein synthesis in simian virus 40-infected simian cells.the pattern of synthesis of non-histone chromosomal proteins in simian virus (sv) 40-infected african green monkey kidney cells was analyzed by polyacryl-amide gel electrophoresis to see whether the changes in chromosomal protein metabolism are involved in the viral-induced synthesis of cellular dna and mrna. during the prereplicative phase of infection, the rate of histone synthesis was decreased until 15 h postinfection, whereas that of non-histone protein synthesis was increased after 5 h pos ...1975163368
virus dna synthesizing ability of t antigen-forming defective sv40 produced by successive undiluted passages.sv40 stocks prepared by successive undiluted passages in african green monkey kidney (gmk) cells contain particles which produce t antigen but not capsid antigen (t partcles). the dna of the t particles was shown to replicate during abortive infection of gmk cells, as indicated by immunofluorescence assay for antigen forming capacity of virus dna. it was also shown that the synthesis of t particle dna was induced in heterokaryons formed by fusion of 3t3 cells transformed by t particles with susc ...1975164518
simian virus 40 dna replication, transcription, and antigen induction during infection with two adenovirus 2-sv40 hybrids that contain the entire sv40 genome.the ad2++hey hybrid virus population produces simian virus 40 (sv40) efficiently during lytic infection, whereas ad2++ley does not, although both hybrids contain a complete sv40 genome. in this report, we demonstrate the synthesis of nonhydrid sv40 dna in ad2++hey-infected vero cells, but only early sv40 rna is transcribed efficiently in ad2++ley-infected cells. ad2++hey induces sv40 u, t, and v antigens during lytic infection of african green monkey kidney cells, whereas ad2++ley induces only s ...1975166206
effect of human leukocyte interferon on vaccinia-and herpes virus-infected cell cultures and monkey corneas.pretreatment of human fibroblast cultures with human leukocyte interferon (hif, 1,000 iu/ml) resulted in a 24-h delay of virus replication after infection with vaccinia virus and herpes simplex virus type 1 and type 2. additional hif treatment 24 h after infection effectively lowered the maximum yield of viral infectivity. equal results were obtained in simian cells with 3,000 iu of hif per ml. the spread of two cell-bound herpesviruses, varicella zoster virus and medical lake macaque herpesviru ...1975166924
temperature-sensitive mutants of simian virus 40 selected by transforming ability.eight temperature-sensitive mutants of simian virus 40 which transform rat cells at 32.5 c but not at 38.5 c have been isolated. all the mutants were also temperature sensitive for replication in african green monkey kidney cells and five of them were classified into a single complementation group. no mutant incapable of transforming rat cells at either temperature was isolated.1975167187
specific immune adherence assay for human hepatitis a antibody application to diagnostic and epidemiologic investigations.a specific immune adherence (ia) test for hepatitis a antibody in human serum was described employing liver extract of marmosets infected with cr326 strain human hepatitis a virus. persons with hepatitis a, but not hepatitis b, developed hepatitis a ia antibody soon after onset of the acute illness and this persisted thereafter. there was very close agreement in the tests for human hepatitis a immune adherence, complement fixing (cf) and neutralizing antibodies. ia antibodies appeared to develop ...1975167376
early cytoplasmic vacuolization of african green monkey kidney cells by early as 3--4 hours after infection with sv40 at a high input multiplicity, african green monkey (cercopithecus aethiops) kidney (agmk) cells developed cytoplasmic vacuolization. at 10--20 hours after infection, the vacuolization reached its maximal level, then disappeared and sv40 specific cytopathic change followed. this vacuolization developed before the synthesis of the specific t and v antigens. this early cytoplasmic vacuolization (ecv) was prevented by preincubating the virus with spec ...1975167691
amino acid and sugar transport incells permissively infected with simian virus 40.transport of alpha-aminoisobutyric acid and 2-deoxy-d-glucose in african green monkey kidney cells was measured 8 to 100 hr following permissive simian virus 40 infection. no differences in transport were detected during the time-period studies, and no significant differences were seen between the apparent michaelis-menten constants of normal and virally infected cells. the absence of transport enhancement in permissive simian virus 40 infection suggests that the augmented transport of viral-tra ...1975165886
[sensitivity of various primate cells and animal viruses to the antiviral activity of human leukocyte interferon (author's transl)].u cells (a permanent, human amnion cell line) were protected against infection with semliki forest virus (sfv) by human interferon (hif) from peripheral leukocytes. despite the usual genus-specific action of interferons, mouse l929 cultures (a permanent mouse fibroblast cell line) were also protected by hif. the antiviral action of hif in six other primate cell cultures was also examined. it is of interest that two lymphoblastoid cell lines, rpmi1788 and kaplan, were insensitive to hif. the sens ...1975170886
isolation of foamy virus from rhesus, african green and cynomolgus monkey leukocytes.foamy virus (fv) was recovered regularly from the leukocyte of rhesus and cynomolgus monkeys and somewhat less often from african green monkey leukocytes. virus was found in virtually all organs of experimentally infected rhesus monkeys. no illness or pathologic abnormalities were noted in these animals or in any of the naturally infected animals in spite of the prolonged period of viral persistence in various organs and tissues.1975171409
[effect of sodium salicylate on the "in vitro" development of rna viruses (author's transl)].sodium salicylate 10(-3) m is able to limit the number of plaques induced by rna viruses on monkey kidney cell cultures (cercopithecus aethiops and macaccus rhesus). however, the inhibition rate changes with the virus type or even the strain used. poliovirus 2, coxsackie b3, echovirus and reovirus 1 were the most susceptible to the inhibitory effect of the drug. latent infection caused by sv5 decreases the antiviral activity of salicylate. the stages of the poliovirus type 2 growth affected by s ...1975171988
simian virus 40-related antigens in three human meningiomas with defined chromosome loss.two out of seven meningiomas tested in early cell cultures by indirect immunofluorescence staining showed simian virus 40 (sv40)-related tumor (t) antigen. in one tumor 90% of the cells were positive. an additional sv40-related antigen (u) was found in 10% of cells of a third tumor. these findings indicate that the meningioma cells showing a positive reaction are transformed by a papova virus that has at least partly the same antigenic properties as sv40 virus. sv40-related viral capsid (v) anti ...1975164660
immune adherence and complement-fixation tests for human hepatitis a. diagnostic and epidemiologic investigations.the reliable propagation of cr326 strain of human hepatitis a virus in saguinus mystax marmosets has permitted the development of specific serum neutralization, complement-fixation (cf), and immune adherence (ia) assays for hepatitis a antigen and antibody. the cf and ia assays were made possible by the use of livers of cr326-infected marmosets as a source of hepatitis a antigen. all assays were shown to be specific for hepatitis a. cases of hepatitis b did not show development of hepatitis a an ...1975173600
biology of sv40 transplantation antigen (trag). i. demonstration of sv40 trag on glutaraldehyde-fixed sv40-infected african green monkey kidney cells. 1976176782
comparative sensitivity of the bgm cell line for isolation of enteric viruses.the bgm line of african green monkey kidney cells was less sensitive than primary rhesus monkey kidney cells or human fetal diploid kidney cells for isolation of certain echoviruses and adenoviruses from fecal specimens.1976178630
characterization of simian cells tranformed by temperature-sensitive mutants of simian virus lines derived from primary african green monkey kidney cells, which had survived lytic infection by wild-type simian virus 40 (sv40) or temperature-sensitive mutants belonging to the a and b complementation groups, were established. these cultures synthesize sv40 tumor (t) antigen constitutively and have been passaged more than 60 times in vitro. the cells released small amounts of virus even at high passage levels but eventually became negative for the spontaneous release of virus. virus ...1976178917
infection of newborn taiwan monkeys (macaca cyclopis) with human adenovirus type 12 and simian virus 40.for testing biological response of newborn taiwan monkeys to infection of human adenovirus type 12 (ad12) and simian virus 40 (sv40), 40 newborn taiwan monkeys were inoculated with ad12, ad12, plus sv40, ad12 supplemented with ad12-induced hamster tumor tissue (ad12 tumor) or control specimens (hela or african green monkey kidney cell lysate). among them 26 survived including 8 newborn monkeys inoculated with control specimens. the survivors were observed for 4 years but no tumor was produced. t ...1975181217
[analysis of the biological effect of city smog extract. v. comparative investigations on the effect of city smog extracts on dna synthesis of syrian hamster kidney and embryonic cells and of african green monkey kidney cells in vitro (author's transl)].we analysed the effect of two samples of city smog extract from bochum and duisburg on dna synthesis of mammalian cells in vitro. as a test system we used tissue cultures of kidney and embryonic cells from the syrian golden hamster and monkey kidney cells from cercopithecus aethiops. dna synthesis of cells was measured by autoradiography using 3h-thymidine. both samples of city smog extract exerted a dose-dependent decrease of the rate of dna synthesis in tissue culture cells. these alterations ...1979121004
morphological observations on the replication of herpesvirus saimiri in monkey kidney cell cultures.owl and african green monkey kidney cell cultures have been infected with 1 p.f.u./cell of herpesvirus saimiri and sample cultures have been taken for examination by electron microscopy at 3 to 6 hourly intervals over a period of 7 days; the experiments were repeated several times. the peculiarly slow replication cycle of herpesvirus saimiri has enabled distinct cytoplasmic and nuclear phases in virus maturation to be clearly distinguished; the overall fine structural features were similar in bo ...1976184242
a scanning electron microscopic study of sv40 infected cells.the surface of the sv40-infected african green monkey kidney (agmk) cells was studied morphologically by scanning electron microscopy. in 24 hr post infection (p.i.), the cell surface was covered with slightly elongated microvilli. the microvilli increased in number. in 96 hr.p.i. most of the cells showed sv40-specific cytopathic effects (cpe). nuclear swellings and the elongation of microvilli were eminent. microvilli were observed projecting with high densities especially on the nuclear portio ...1976184324
surveys of simian malaria in japan.the survey of simian malaria in japan which had been conducted since 1965 showed that no japanese monkeys (macaca fuscata fuscata and macaca fuscata yakui) were infected with malaria parasites. most interestingly, however, babesia sp. was found in 4 (4.3%) of 93 macaca fuscata yakui which had been reared in a monkey park. as for the monkeys imported from abroad, plasmodium was only found in macaca irus from southeast asia, namely, p. inui (14.1-25.0%), p. cynomologi (0.6, 3.2%) and p. coatneyi ( ...1979116437
linkage of the genes for thymidine kinase and galactokinase in the african green monkey and the chimpanzee. 1976185024
two adenovirus type 2-simian virus 40 hybrid populations that contain the entire sv40 genome differ in their ability to induce sv40 transplantation immunity in hamsters but not in mice.ad2++ hey and ad2++ ley are two adenovirus 2(ad2)-simian virus 40 (sv40) hybrids distinguished by differences in the efficiency with which they produce sv40 progeny in lytically infected african green monkey kidney cells. these virus populations are composed of nonhybrid ad2 and hybrid virions, the majority of which contain more than 1 unit of sv40 dna. the ad2++ hey and ley populations also differ in their ability to induce sv40 transplantation immunity in rodents. only ad2++ hey induces sv40 t ...1976185429
serum lipid and lipoprotein responses of six nonhuman primate species to dietary changes in cholesterol levels.the response of serum lipids and lipoproteins to different levels of cholesterol in the diet was studied in chimpanzee (pan troglodytes), rhesus (macaca mulatta), green (cercopithecus aethiops), patas (erythrocebus patas), squirrel (saimiri sciurea) and spider (ateles sp.) monkeys. five animals of each species were fed increasing amounts of dietary cholesterol (0.05% to 1.5% w/w) for 3-week periods; between each experimental diet, the animals were fed a basal diet without cholesterol for a simil ...1976186573
activation of epstein-barr virus in hybrid cells.human-primate hybrid cell lines were established by fusion of african green monkey kidney cells (vero) with lymphoblastoid cells from patients with infectious mononucleosis (im)(imk101) and from burkitt's lymphoma culture (hr1k). both epstein-barr virus (ebv)-specific antigens and ebv particle-containing cells increased in the hybrid lines (imk1-1/vero,hr1k/vero). treatment of the hybrids with 5-bromodeoxyuridine induced more antigen-positive and more virus-containing cells. ebv could be activat ...1977192898
properties of permissive monkey cells transformed by uv-irradiated simian virus 40.african green monkey cells (cv1 line) were infected with uv-irradiated simian virus 40 (sv40), and permissive lines of stably transformed cells were established. these cell lines display the sv40 t-antigen and the growth characteristics typical of nonpermissive transformed cells (e.g., reduced cell density inhibition, reduced serum dependence, ability to overgrow normal cells, and colony formation in soft agar). the level of permissiveness to superinfecting sv40 is fully comparable with that of ...1977194053
behavioral effects of low, acute doses of d-amphetamine on the dyadic interaction between mother and infant vervet monkeys (cercopithecus aethiops) during the first six postnatal months.doses of d-amphetamine sulfate (0.1, 0.15, and 0.2 mg/kg body weight) were given to adult monkeys in mother-infant pairs. the fundamental parental care behavior pattern was disrupted and the mother became isolated in a socially withdrawn phase. she did not respond to the calling signals of the infant and showed behavior in which stereotyped self-grooming and/or 'staring' into space were predominant. the reactions of the infant to this amphetamine-induced behavior were different in the two experi ...1979115045
portacaval shunt and whole-body cholesterol metabolism in the cholesterol-fed african green monkey. 1979113786
the effects of orbitofrontal lesions on the aggressive behavior of vervet monkeys (cercopithecus aethiops sabaeus). 1979113236
characterization of simian virus 40 transformed african green monkey cells (cv-1). i. defective virion and viral genome.several clones of sv40 transformed cv-1 cells have been characterized for the production of t- and v-antigens and for the state of viral genome. the transformed cv-1 cells failed to produce infectious virions as assayed after sonication or cocultivation and fusion with normal cv-1 cells, and were resistant to super-infection by sv40. some clones of the transformed cells contained v-antigens. the population of v-antigen positive cells varied from 0 to 100% depending on the passage number while th ...1977196162
[demonstration by immunofluorescence of adenohyphysis corticotropin and melanotropin cells in erythrocebus patas, cercopithecus aethiops and papio hamadryas].using antibodies against synthetic corticotropic hormones (1-24 acth and 17-39 acth), and melanotropic hormones (alpha-msh and beta-msh), it is possible to identify corticotropic and melanotropic cells in the adenohypophysis of three species of monkeys : erythrocebus patas, cercopithecus aethiops and papio hamadryas. the corticotropic cells are numerous in the anterior lobe in both the adult and infant male and female monkeys of these three species. the intermediate lobe reacts with antibodies a ...1977196718
structure and formation of circular dimers of simian virus 40 dna.most of the viral dna extracted from simian virus 40 (sv40)-infected african green monkey kidney cells consists of circular molecules about 5.3 kilobases in contour length. however, about 1% of the viral dna was found to occur as closed circular dimers that appeared to be formed, preferentially, late in infection. the monomeric units of dimers were organized in a head-to-tail, tandem arrangement; moreover, the monomeric units were not defective; i.e., they lacked deletions or other rearrangement ...1977198580
characterization of simian virus 40-transformed african green monkey cells (cv-1). ii. semipermissive character for viral replication. 1977200829
histone synthesis during infection of monkey kidney cells with simian virus 40.the synthesis of histones during lytic infection of bsc-1 (african green monkey kidney) cells with sv40 has been investigated. the synthesis of all five classes of histones was stimulated, and all classes appeared to be stimulated to the same extent. the increase in rate of histone synthesis in response to sv40 infection was detectable several hours before sv40 dna synthesis was measureable, and the rate of histone synthesis decreased at a time when sv40 dna synthesis was occuring at a maximal o ...1977200893
nonhuman primates: laboratory animals of choice for neurophysiologic studies of sleep.a systematic study was made of several nonhuman primates to learn more about their relative usefulness for studies of sleep. species studied included the guinea baboon (papio papio), kenya or yellow baboon (p cynocephalus), olive baboon (p anubis), sacred baboon (p hamadryas), vervet monkey (cercopithecus aethiops), rhesus monkey (macaca mulatta), bonnet monkey (m radiata), crab-eating monkey (m fascicularis), patas monkey (erythrocebus patas), chimpanzee (pan troglodytes), mongoose lemur (lemur ...1977201798
highly reiterated sequences of simiansimiansimiansimiansimian.a 172-base pair segment of dna that is repeated several million times in the genome of the african green monkey has been characterized. sequence analysis revealed that the many repeats of this complex unit are not all identical but represent a set of closely related segments: sequence divergence occurs at various positions in the segment in a nonrandom manner. the uncloned segment obtained from monkey dna is compared with a cloned segment of the same dna which was recombined into the genome of s ...1978205944
transformation of african green monkey kidney cells with the rf strain of human papovavirus bkv. 1978206005
cae i: an endonuclease isolated from the african green monkey with properties indicating site-specific cleavage of homologous and heterologous mammalian dna.component alpha dna is a highly repetitive sequence that comprises nearly a quarter of the african green monkey (cercopithecus aethiops) genome. a previous microbial restriction enzyme analysis showed that the repeat structure of component alpha dna is based upon a monomeric unit of 176 +/- 4 base-pairs. an endonuclease, provisionally termed case i, has been isolated from african green monkey testes that cleaves component alpha dna into multimeric segments based upon the same repeat periodicity ...1978206873
incorporation of lysine into y base of phenylalanine trna in vero cells.vero cells, a line derived from african green monkey kidney, contains a hypermodified base, called y, adjacent to the 3' end of the anticodon of trnaphe. two types of evidence are presented suggesting that lysine is involved in biosynthesis of y base in these cells. first, when vero cells are starved for lysine, a new, early-eluting species of trnaphe which lacks the fully modified y base can be detected by reversed phase chromatography (rpc-5). after addition of lysine to the medium, this new s ...1979111226
arrangement of a highly repeated dna sequence in the genome and chromatin of the african green monkey.the dna of the african green monkey contains three components that are distinguishable by the kinetics of reassociation. the rapidly reassociating component represents about 20% of the total dna and is composed almost entirely of a sequence (agmr(hindiii)-1) which is repeated 6.8 x 10(6) times. the majority of the agmr(hindiii)-1 sequences are organized in long tandem repeats of a segment of 172 base pairs in length. however, a fraction of the agmr (hindiii)-1 sequences is interspersed with anot ...1979109436
the turnover of trnas microinjected into animal cell-mediated microinjection has been used to study trna turnover in sv3t3 mouse cells and tc7 cells, an african green monkey kidney line. the turnover of endogenous trna, measured by labeling with 3h-methionine, was first-order with half-lives of approximately one day in sv3t3 and two days in tc7 cells. 32ptrna isolated from e. coli or tc7 cells turned over at the same rate as endogenous trna when injected into either sv3t3 or tc7 cells. this demonstrates that cellular processes, not proper ...1978214762
comparative sensitivity of various cell culture systems for isolation of viruses from wastewater and fecal efforts to define the most sensitive cell culture systems for recovery of viruses from wastewaters, 181 samples were inoculated in parallel into tube cultures of various cell types and were plaqued in bottle and petri dish cultures of three types of monkey kidney cells. polioviruses were recovered most frequently in the rd line of human rhabdomyosarcoma cells, group a coxsackieviruses in rd and human fetal diploid kidney (hfdk) cells, group b coxsackieviruses in the bgm line of african green ...1978215087
reduction of herpes simplex virus-type 2 plaque formation by urea.the effect of urea on plaque formation by herpes simplex virus type 2 (hsv-2) was examined in two systems at concentrations within or approximating the range found in human urine. approximately 7--10 mg urea/ml, added 2, 4, or 8 hours after infection, reduced plaque formation by 50% in african green monkey kidney cells. the growth of this system was affected slightly by continuous treatment with urea at 7--10 mg/ml. plaque formation was reduced in the monkey kidney system, albeit diminishingly, ...1978215721
identity of euchromatic bands from man to cercopithecidae (cercopithecus aethiops, cercopithecus sabaeus, erythrocebus patas, and miopithecus talapoin).the karyotypes of four species of cercopithecidae: cercopithecus aethiops tantalus, c. sabaeus, erythrocebus patas, and miopithecus talapoin are analysed with nearly all the banding techniques. they are compared with each other, and with the karyotypes of the baboon p. papio and with that of man. it can be concluded that the quasi-totality or the totality of the euchromatin is common to all, but has undergone structural rearrangements, generally detectable. the heterochromatin, defined by c-band ...1978104920
ultrastructure of sensory nerve terminals in the penis in green monkey (cercopithecus aethiops sabaeus).tje paper describes the ultrastructure of axons in the endings of various types from the corium in the glans penis in green monkey. in the meissner's endings the axons are mostly completely enveloped in the plasma of schwann cells. they contain numerous mitochondria which are partially vacuolated or are quite converted into vacuoles. next, there are pseudomyelinated figures, light vesicles and further organelles. in the papillar simple glomerular endings with accumulation of schwann cells there ...197799893
antibodies to guinea pig lymphokines. vii. reactivity with products of lymphoid and nonlymphoid was shown previously, that an antiserum directed against highly purified fractions of migration inhibitory factor inhibits delayed hypersensitivity reactions in vivo and in vitro. using radiolabeling techniques we determined that the anti-lymphokine serum reacted primarily with three lymphocyte activation products (m.w. 60,000, 45,000, and 30,000) all of which had a similar isoelectric point of 5.2. the cellular origin of this material was investigated. it was found that activated b cells, b ...197899476
a simple, rapid syncytial-inhibition test for antibodies to bovine leukemia virus.cocultivation of equal numbers of cells from a fetal lamb kidney line infected with bovine leukemia virus and african green monkey (vero) cells results in the rapid production of syncytia. the effect was blocked or inhibited by serum containing antibodies to bovine leukemia virus. a serological test based on syncytial inhibition was compared to the agar gel immunodiffusion test and the modified direct complement fixation test for the detection of bovine leukemia virus antibodies in sera from leu ...1978217507
demonstration of an analogue to human pregnancy zone protein in the african green monkey.a serum protein in the grivet (cercopithecus aethiops), immunologically cross-reacting with human pregnancy zone protein (pzp), is found in males as well as in nonpregnant females. the electrophoretic mobility, antigenic cross-reactivity, molecular weight, and carbohydrate structure appear analogous to pzp; however, contrary to the situation in man, exogenous estrogens cause a fall in serum concentration of the protein.197994099
leucocyte migration inhibition test as an index of immunological response to measles virus ii. immunogenic activity of measles virus.experiments discussed in this paper aimed at obtaining, using cercopithecus aethiops monkeys information on immunogenic activity of measles virus as well as kinetic and persistence of immunological reaction at active and inactivated virus.197775673
Displaying items 1 - 100 of 3231