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titration behavior of individual tyrosine residues of myoglobins from sperm whale, horse, and red kangaroo.the titration behavior of individual tyrosine residues of myoglobins has been studied by observing the ph dependence of the chemical shifts of czeta and cgamma of these residues in natural abundance of 13c fourier transform nmr spectra (at 15.18 mhz, in 20-mm sample tubes, at 37 degrees) of cyanoferrimyoglobins from sperm whale, horse, and red kangaroo. a comparison of the ph dependence of the spectra of the three proteins yielded specific assignments for the resonance of tyr-151 (sperm whale) a ...19768455
oxygen affinity and 2,3-diphosphoglycerate in blood of australian marsupials of differing body size.oxygen carriage and 2,3-diphosphoglycerate (2,3-dpg) levels have been measured in the blood of seven species of australian marsupials ranging in size from 35 to 0.03 kg. they were red and grey kangaroos, wallaroo, tammar wallaby, brush-tailed possum, potoroo, and brown marsupial mouse. oxygen affinity decreased with decrease in adult body size, standard p50 (at 36 c) varying from 24.6 torr in the largest (red kangaroo) to 41.9 torr in the smallest (brown marsupial mouse). the relationship betwee ...197724866
acid and alkaline forms of the higher oxidation state of kangaroo, horse, and sperm whale myoglobin.myoglobin(iv), the derivative of myoglobin at the formal oxidation state iv, prepared from kangaroo (megaleia rufa), horse, or sperm whale myoglobin, when cooled to liquid nitrogen temperature, assumes acid and alkaline forms with different optical spectra. the essential features of the optical spectra of the acid forms are the same as those of leghemoglobin(iv) and are very similar to those of optical spectra of the red higher oxidation states of catalases and peroxidases. this shows that the c ...197827521
the use of marsupial x eutherian somatic cell hybrids to study marsupial cell surface antigens.buck and bodmer (1976) have developed a technique for identifying an antigen on the surface of human x mouse somatic cell hybrids, specified by a gene on a particular human chromosome. we have successfully adapted this technique to a study of marsupial cell surface antigens. somatic cell hybrids between macropus rufus (marsupialia) lymphocytes and the mouse cell lines pg19 and 1r were injected intraperitoneally into mice of the same inbred strain from which the above cell lines were derived (c57 ...1978572214
progamotaenia ruficola sp. n. (cestoda: anoplocephalidae) from the red kangaroo, macropus rufus (marsupialia).progamotaenia ruficola sp. n. is described from the red kangaroo (macropus rufus) from new south wales, australia. it is distinguished from other similar species having paired, nondiverticulate uteri in each prolottis: progamotaenia festiva (rudolphi 1819), p. diaphana (zschokke 1907), and p. macropodis beveridge 1976, by the extremely broad, straight-edged velum, the lack of an external seminal vesicle, the cirrus armature, the number of testes, the lack of vaginal atrophy following inseminatio ...1978641671
the amino-acid sequence of kangaroo pancreatic ribonuclease.red kangaroo (macropus rufus) ribonuclease was isolated from pancreatic tissue by affinity chromatography. the amino acid sequence was determined by automatic sequencing of overlapping large fragments and by analysis of shorter peptides obtained by digestion with a number of proteolytic enzymes. the polypeptide chain consists of 122 amino acid residues. compared to other ribonucleases, the n-terminal residue and residue 114 are deleted. in other pancreatic ribonucleases position 114 is occupied ...1978658039
cholangitis associated with species of progamotaenia (cestoda: anoplocephalidae) in the bile ducts of marsupials.gross and histopathologic changes due to infection with progamotaenia festiva and p. effigia in the bile ducts of the marsupials, macropus rufus, m. giganteus, m. fuliginosus, lagorchestes conspicillatus, vombatus ursinus and lasiorhinus latifrons are described. the integrity of the bile duct mucosa was not impaired but varying degrees of hyperplasia and hypertrophy of mucosa and mucosal glands, inflammatory infiltration and fibrosis were found. portal fibrosis was the prominent reaction in lasi ...1978691133
the amino-acid sequence of the alpha-crystallin a chains of red kangaroo and virginia opossum.the amino acid sequence of the a chain of the eye lens protein alpha-crystallin from the red kangaroo (macropus rufus) was completely determined by manual edman degradation of tryptic, thermolytic and cyanogen bromide peptides. the sequence of the alpha-crystallin a chain from the virginia opossum (didelphis marsupialis) was deduced from amino acid analyses and partial edman degradation of peptides. the 173-residue a chains of kangaroo and opossum differ in six positions, whereas comparison with ...1976964255
pneumocystis carinii infections in zoo animals.pneumocystis carinii was found to be present in the lungs of twenty-three zoo animals in the netherlands. the following species were represented: red kangaroo, common tree shrew, senegal-galago, demidoff's-galago, brown howler monkey, woolly monkey, long-haired spider monkey, white-eared marmoset, chimpanzee, three-toed sloth, palm squirrel, red panda, fennec fox, tree hyrax and large-toothed hyrax.19751079994
effects of dehydration on body-water distribution in desert kangaroos.the effect of dehydration on the distribution of water in the bodies of two species of desert kangaroos, the red kangaroo megaleia rufa and the euro macropus robustus, has been examined. the volumes of various body-fluid compartments were determined in normally hydrated animals and then after the kangaroos had been dehydrated until body weight declined to 80% of the initial weight. the fluid compartments examined were total body water, plasma volume, intracellular volume (cellular and gut water) ...19751147052
detection by allozyme electrophoresis of cryptic species of hypodontus macropi (nematoda: strongyloidea) from macropodid marsupials.allozyme electrophoresis of 98 hypodontus macropi from eight different species of hosts using 24 enzymes revealed a complex of at least six sibling species, with 15-50% fixed genetic differences between taxa. except for the taxon parasitizing macropus rufus/m. robustus, pairs of parasite taxa were, in each case, sympatric at each locality examined, thus supporting the conclusion that they represent valid species. the existence of a series of host-specific nematode taxa explains many of the incon ...19921639562
1h nmr spectroscopic survey of plasma and erythrocytes from selected marsupials and domestic animals of australia.1. 1h nmr spectra were acquired from whole plasma, intact erythrocytes, and ultrafiltrates of erythrocytes from nine native and eight introduced (domestic) australian animals; single-pulse, spin-echo and 2-dimensional spectra were obtained. the aim was to detect and at least semi-quantify metabolites in the samples and compare the profiles amongst the species. 2. the australian natives that were studied were all marsupials: greater brown bandicoot; bettong; eastern grey kangaroo; red kangaroo; k ...19911769206
effect of phosphate-regulating hormones on plasma composition, cardiovascular function, and parotid salivary phosphate secretion in red kangaroos (macropus rufus).the effects of administration of phosphate-regulating hormones on plasma composition, cardiovascular function, and secretion of phosphate and other electrolytes in parotid saliva were investigated in anesthetized red kangaroos. plasma [po4] was elevated by intravenous injections of 1,25-dihydroxycholecalciferol (1,25(oh)2d3) at 5 or 12.5 nmol/12 hr for 72 hr but was unaltered by intravenous or intracarotid infusion of either salmon or porcine calcitonins at rates up to 3.2 iu min-1 for 60 min or ...19912026317
metabolic effects of cortisol, acth, adrenalin and insulin in the marsupial sugar glider, petaurus breviceps.the effects of cortisol, acth, adrenalin and insulin on indices of carbohydrate, fat and protein metabolism were investigated in the conscious marsupial sugar glider petaurus breviceps. short-term i.v. infusion of cortisol at dose rates of 0.02, 0.2 and 1.0 mg/kg per h caused the plasma glucose concentration to rise sharply from the normal range of 3.3-4.4 to 8.1-8.7 mmol/l at the end of the infusion period without significant alteration in plasma free fatty acid (ffa), amino acid or urea concen ...19902174452
x-ray crystallography of incisor enamel from red kangaroo.x-ray powder diffraction of red kangaroo (macropus rufus) dental enamel, with a relative density greater than 2.60, showed a longer a-axis length of the apatite unit cell, a = 9.462 a, a shorter c-axis length, c = 6.877 a, and a smaller crystallite size, ca. 185 a, than those of human dental enamel. the a-axis of red kangaroo dental enamel was one of the longest among the values reported previously, whereas the c-axis was one of the shortest. the crystallite size was almost equal to that of dent ...19902374023
secretion by the mandibular gland of the red kangaroo (macropus rufus) during isoprenaline infusion.intracarotid infusion of isoprenaline, either alone or in combination with acetylcholine infusion was used to stimulate salivation by the mandibular glands of anaesthetized red kangaroos. isoprenaline alone (0.20-1.25 eta mol.kg-1.min-1) elicited flow rates ranging from 0.014 to 0.239 ml.min-1 (1.21-28.1 microliters.g gland-1.min-1). salivary concentrations of sodium, chloride, phosphate and urea were negatively correlated with flow, whereas potassium, calcium, magnesium, hydrogen ion, bi-carbon ...19892607021
characteristics of cardiac action potentials in marsupials.standard microelectrode techniques were used to record action potentials from single atrial, ventricular and purkinje fibers of hearts taken from three species of marsupial (macropus rufus, macropus robustus and macropus eugenii) and from dogs, sheep and guinea-pigs. the major electrophysiological parameters of marsupial potentials were qualitatively similar to the values for placental mammals. the grouped data for ventricular action potentials from studies on 6 adult male red kangaroos (macropu ...19892715459
the myoglobin protein radical. coupling of tyr-103 to tyr-151 in the h2o2-mediated cross-linking of sperm whale myoglobin.sperm whale metmyoglobin, which has tyrosine residues at positions 103, 146, and 151, dimerizes in the presence of h2o2. equine metmyoglobin, which lacks tyr-151, and red kangaroo metmyoglobin, which lacks tyr-103 and tyr-151, do not dimerize in the presence of h2o2. the dityrosine content of the sperm whale myoglobin dimer shows that it is primarily held together by dityrosine cross-links, although more tyrosine residues are lost than are accounted for by dityrosine formation. digestion of the ...19883182873
a comparison of the intrinsic fluorescence of red kangaroo, horse and sperm whale metmyoglobins.several metmyoglobins (red kangaroo, horse and sperm whale), containing different numbers of tyrosines, but with invariant tryptophan residues (trp-7, trp-14), exhibit intrinsic fluorescence when studied by steady-state front-face fluorometry. the increasing tyrosine content of these myoglobins correlates with a shift in emission maximum to shorter wavelengths with excitation at 280 nm: red kangaroo (tyr-146) emission maximum 335 nm; horse (tyr-103, -146) emission maximum 333 nm; sperm whale (ty ...19863730392
nadh-methemoglobin reductase activity in the erythrocytes of newborn and adult mammals.nadh-mr activity was measured in the erythrocytes of newborn and adult horses, pigs, cattle, sheep, goats, red kangaroos, fruit bats, rats, mice, rabbits and humans. our results fail to support an earlier hypothesis that higher nadh-mr activity may be an adaptation to increased ruminal nitrite production leading to accelerated oxidation of fetal hemoglobin.19863780953
provisional mapping of the gene for a cell surface marker, ga-1, in the red-necked wallaby macropus rufogriseus.a series of m. rufogriseus-mouse somatic cell hybrids was constructed and analysed cytologically, enzymatically and immunologically. a monoclonal antibody, ga-1, was prepared against an m. rufogriseus cell surface antigen on an m. rufogriseus-mouse somatic cell hybrid. a gene determining the expression of this antigen was provisionally assigned to the long arm of the m. rufogriseus chromosome 3. the monoclonal antibody also reacted with an m. rufus (red kangaroo)-mouse somatic cell hybrid contai ...19853834888
klossiella (apicomplexa, klossiellidae) in petaurid and macropodid marsupials in australia.six new species of klossiella are described in the kidneys of australian marsupials: klossiella rufogrisei in bennett's wallaby, macropus rufogriseus; klossiella rufi in the red kangaroo, macropus rufus; klossiella thylogale in the red-bellied or tasmanian pademelon, thylogale billardierii; klossiella beveridgei in the spectacled hare-wallaby, lagorchestes conspicillatus; klossiella bettongiae in the tasmanian bettong, bettongia gaimardi; and klossiella schoinobatis in the petaurid greater glide ...19853930707
proteins of marsupial erythrocyte membranes.the proteins of erythrocyte membranes from the red kangaroo, western grey kangaroo, eastern grey wallaroo (euro), red-necked wallaby, tammar wallaby, and brush-tail possum have been fractionated on acrylamide gels in the presence of sodium dodecyl sulfate. the pattern of proteins was remarkably similar between the different marsupial species. the pattern of coomassie blue-staining proteins in the membranes of these species was also very similar to that of the human erythrocyte membrane. however, ...19854051902
thermoregulatory responses of the arid zone kangaroos, megaleia rufa and macropus robustus. 19734147795
carbohydrates of the prostate of two australian marsupials, trichosurus vulpecula and megaleia rufa. 19744412309
blood chemistry and gastro-intestinal changes in the developing red kangaroo (megaleia rufa, desmarest). 19725065475
absence of countercurrent exchange of oxygen, carbon dioxide, hydrogen ions or glucose between the arterial and venous blood in the spermatic cords of rams and two marsupials (macropus eugenii and megaleia rufa). 19695357945
glyceraldehyde-3-phosphate dehydrogenase from the red kangaroo (megaleia rufa): purification and the amino acid sequence around a reactive cysteine. 19715579442
prolactin and luteinizing hormone receptors in marsupial corpora lutea: relationship to control of luteal function.prolactin and lh receptor concentrations in tammar wallaby corpora lutea (cl) have been examined and related to the control of luteal function in this and other marsupial species. during embryonic diapause, quiescent cl contained high concentrations of prolactin receptors. this was consistent with an earlier suggestion that prolactin may act directly on the cl to maintain its quiescent state. however, despite an apparent seasonal change in the mechanism by which the cl is maintained in quiescenc ...19826276490
detection and control of rotavirus infections in zoo animals.fecal specimens from 15 exotic animal species, with and without diarrhea, were examined for the presence of rotavirus, bacterial enteropathogens, and intestinal parasites. a commercial enzyme-linked immunosorbent assay was used to detect antigens of rotavirus. rotavirus was detected in the feces of 20 (57%) of 35 of the animals, which included addax (addax nasomaculatus), nyala (tragelaphus angasi), saiga (saiga tatarica), white-tailed gnu (connochaetus gnou), greater kudu (tragelaphus strepsice ...19836315660
marsupial neurohypophysial hormones: identification of mesotocin, lysine vasopressin, and phenypressin in the quokka wallaby (setonix brachyurus).the neurohypophysial hormones of the quokka wallaby (setonix brachyurus) have been isolated through preparative high pressure liquid chromatography (hplc). one oxytocin-like and two vasopressin-like peptides have been found. these peptides have been characterized by their amino acid composition and by their retention time in hplc. mesotocin ([i1e8]-oxytocin) has been identified by amino acid composition, polar partition chromatography, high pressure liquid chromatography with a reversed phase co ...19836618161
mapping a marsupial x chromosome using kangaroo-mouse somatic cell hybrids.a series of marsupial-eutherian somatic cell hybrids was produced by fusion between lymphocytes from the red kangaroo (macropus rufus) and hprt-deficient mouse cells. the hybrids lost marsupial chromosomes and could therefore be used to map marsupial genes. several of the hybrids contained a complete red kangaroo x chromosome and expressed the kangaroo form of the enzymes hprt, g6pd, and pgka. a number of hprt-deficient revertant cell lines were derived from the hybrids. these possessed a variet ...19817194766
an autosomal gene assignment in a marsupial: the gene for ldh-a is on chromosome 5 of the red kangaroo, macropus rufus.a number of somatic cell hybrids between red kangaroo (macropus rufus) and mouse cells, which lose marsupial chromosomes, were found to express the kangaroo form of ldh-a. concordance between the expression of marsupial ldh-a and the presence of chromosome 5 in the hybrid cells and selected subclones enabled the gene for ldh-a to be assigned to this chromosome. this is the first autosomal gene assignment in a marsupial and should prove important for chromosome mapping in the red kangaroo and in ...19817332531
comparative studies of alpha-lactalbumin and lysozyme: the proteins of kangaroo (megaleia rufa and macropus giganteus) and horse (equus caballus).as part of a study of the 'whey' proteins of various mammals, a comparison is made of the alpha-lactalbumins and lysozymes of the kangaroo and horse. in the milk of the red kangaroo (megaleia rufa) there is only one alpha-lactalbumin and it occurs throughout lactation, but no lysozyme has been detected. there are two alpha-lactalbumins in the milk of the grey kangaroo (macropus giganteus), one, designated alpha-lactalbumin zone b, is present throughout lactation; the second, designated alpha-lac ...19807366582
an outbreak of pasteurellosis in kano zoo.an outbreak of pasteurellosis caused by pasteurella multocida is reported in which six elands (taurotragus oryx), a gnu (connochaetes taurinus albojubatus), a zebra (equus bruchelli), five kangaroos (macropus rufus), an ostrich (struthio camelus camelus) and a bateleur eagle (terathopius ecaudatus) died in kano zoological gardens toward the end of the rainy season of 1978. confirmation was based on cultural isolation of p. multocida. analysis of likely factors that could have contributed to the ...19807373722
phosphoglycerate kinase pseudogenes in the tammar wallaby and other macropodid marsupials.phosphoglycerate kinase (ec 2.7.2.3; pgk) exists in two forms in marsupials. pgk1 is an x-linked house-keeping enzyme, and pgk2 is a mainly testis-specific enzyme under autosomal control. we have used pgk1 probes derived from two closely related species of macropodid marsupials (kangaroos and wallabies) to demonstrate the existence of a large family of pseudogenes in the tammar wallaby (macropus eugenii). over 30 fragments are detectable after taq digestion. we estimate that there are 25-30 copi ...19948000135
running springs: speed and animal size.trotting and hopping animals use muscles, tendons and ligaments to store and return elastic energy as they bounce along the ground. we examine how the musculoskeletal spring system operates at different speeds and in animals of different sizes. we model trotting and hopping as a simple spring-mass system which consists of a leg spring and a mass. we find that the stiffness of the leg spring (k(leg)) is nearly independent of speed in dogs, goats, horses and red kangaroos. as these animals trot or ...19938294853
diffusional water permeability of mammalian red blood cells.an extensive programme of comparative nuclear magnetic resonance measurements of the membrane diffusional permeability for water (pd) and of the activation energy (ea,d) of this process in red blood cells (rbcs) from 21 mammalian species was carried out. on the basis of pd, these species could be divided into three groups. first, the rbc's from humans, cow, sheep and "large" kangaroos (macropus giganteus and macropus rufus) had pd values approximately 5 x 10(-3) cm/s at 25 degrees and 7 x 10(-3) ...19958590380
a new family of entodiniomorph protozoa from the marsupial forestomach, with descriptions of a new genus and five new species.a unique group of entodiniomorph protozoa was found in forestomach contents from quokka (setonix brachyurus), western grey kangaroo (macropus fuliginosus), red kangaroo (macropus rufus) and euro (macropus robustus erubescens). a new genus, macropodinium n.g., containing five new species, is described. three species are described from forestomach contents of the quokka: macropodinium baldense n. sp., macropodinium moiri n. sp. and macropodinium setonixum n. sp. a single species, macropodinium enn ...19968768432
molecular population genetics of the red kangaroo (macropus rufus): mtdna variation.the genetic population structure of a large, wide-ranging marsupial, the red kangaroo (macropus rufus) was assessed using sequence and haplotype frequency data of mitochondrial dna (mtdna) from locations across the species range in australia. results from sequence data revealed extensive haplotype diversity within the red kangaroo (32/34 sequences were unique). sequence diversity was distributed within rather than between geographical regions across the species range. genetic connectivity across ...19989640648
jaw-closing muscles of kangaroos express alpha-cardiac myosin heavy chain.the masseter muscle of eutherian grazing mammals typically express beta or slow myosin heavy chain (myhc). myosins in the masseter of 4 species of kangaroos and a slow limb muscle of one of them were compared with their cardiac myosin by pyrophosphate and sodium dodecyl sulphate (sds) gel electrophoresis, immunoblotting and immunohistochemistry. it was found that ventricular muscle contains three isoforms homologous to v1 (alpha-myhc homodimer), v2 (heterodimer) and v3 (beta-myhc homodimer) of e ...200011227794
erythrocyte osmotic fragility of red (macropus rufus) and grey (macropus fuliginosus and macropus giganteus) kangaroos and free-ranging sheep of the arid regions of australia.the mean corpuscular fragility (mcf) of erythrocytes may reflect phylogenetic characteristics as well as an animal's ability to respond to the osmotic challenges associated with cyclic dehydration and rehydration. this type of ecophysiological stress is commonly encountered by animals living in arid regions and low mcf may contribute to their ability to survive and thrive in these xeric habitats. the eastern grey kangaroo has only in recent times extended its range into the arid zone, and is con ...200111263725
mutation scanning analysis of sequence heterogeneity in the second internal transcribed spacer (rdna) within some members of the hypodontus macropi (nematoda: strongyloidea) complex.single-strand conformation polymorphism analysis was employed to investigate sequence variation in the second internal transcribed spacer (its-2) of nuclear ribosomal dna within and among individuals representing three operational taxonomic units (otus) of hypodontus macropi from different species of australian macropodid marsupials. of the 96 nematodes analysed, totals of 3 (otu1 from petrogale persephone), 10 (otu2 from macropus robustus) and 7 (otu9 from macropus rufus) representative individ ...200111358128
single-strand conformation polymorphism analysis of genetic variation in labiostrongylus longispicularis from kangaroos.single-strand conformation polymorphism (sscp) analysis was employed to screen for sequence heterogeneity in the second internal transcribed spacer (its-2) of ribosomal (r) dna of labiostrongylus longispicularis, a parasitic strongylid nematode occuring in some species of kangaroo in different geographical regions of australia. the results showed that most of the nematodes screened had different sscp profiles, which were subsequently shown to correspond to polymorphisms and/or an indel in the it ...200111465490
cloning of the red kangaroo (macropus rufus) follicle stimulating hormone beta subunit.the cdna encoding the follicle stimulating hormone beta subunit (fsh-beta) was isolated from a red kangaroo pituitary cdna library by using a porcine probe and the nucleotide sequence for the coding region was determined. the highest degree of deduced amino acid sequence identity (91%) was observed between the red kangaroo and another marsupial, the brushtail possum (trichosurus vulpecula), followed by eutherian species (76%, 75% and 74%, respectively, for pig, mouse and sheep). based on the ded ...199811596876
redescription of zoniolaimus mawsonae beveridge, 1983 (nematoda: strongyloidea) and the description of z. latebrosus n. sp. from the red kangaroo macropus rufus (marsupialia: macropodidae) based on morphological and molecular data.this study shows that the description of z. mawsonae given by beveridge (1983) represented a composite of two species. hence, z. mawsonae beveridge, 1983 is re-described and a new species, z. latebrosus, is described. males of the two species differ in the lengths of their spicules (0.94-1.23 mm in z. mawsonae compared with 1.53-1.95 mm in z. latebrosus) and in several characteristics of the bursa and genital cone. females of the two species can be identified based on the shape of the posterior ...200211912339
effects of p-chloromercuribenzene sulfonate on water transport across the marsupial erythrocyte membrane.the effects of exposure of red blood cells (rbc) of three species of marsupial to a mercury-containing sulfhydryl-modifying reagent, p-chloromercuribenzene sulfonate (pcmbs), on the water diffusional permeability ( p (d)) of their membranes were monitored by using an mn(2+)-doping (1)h nuclear magnetic resonance (nmr) technique at 400 mhz. for koala ( phascolarctos cinereus), rbc the maximal inhibition was reached at 37 degrees c in 60 min with 1 mmol.l(-1) pcmbs or in 15-30 min with 2 mmol. l(- ...200212192513
functional capacities of marsupial hearts: size and mitochondrial parameters indicate higher aerobic capabilities than generally seen in placental mammals.this study of marsupial hearts explored the aerobic capacities of this group of mammals; recent information suggests that marsupials possess higher aerobic abilities than previously accepted. characteristics such as heart mass, mitochondrial features and capillary parameters were examined. a comprehensive study of the heart of red kangaroos was included because of the high maximum oxygen consumption of this species. goats were also included as a reference placental mammal. marsupials have a hear ...200312898164
coccidia in wild populations of the red kangaroo, megaleia rufa (desmarest), and the grey kangaroo, macropus canguru (muueller). 196414124276
a survey of the endoparasites of the red kangaroo megaleia rufa (desmarest). 196414227630
oral biology and disorders of chiroptera, insectivores, monotremes, and marsupials.oral disease occurs in all species, from the tiny marsupial mouse to the large red kangaroo, and from the smallest mole to the largest bat. although there has been little research done in most of the species in this article, the whole range of dental and oral diseases has been recorded, including periodontal disease, caries, and many variations in position and number of teeth. hopefully, the interest in these species will continue and further study will enable us to understand these diseases mor ...200314534972
comparative morphology of the tapetum lucidum (among selected species).the phenomenon of 'eye-shine' is seen in a variety of animal species, and is generally thought to be related to the presence of an intraocular reflecting structure, the tapetum lucidum. the tapetum lucidum is a biologic reflector system that is a common feature in the eyes of vertebrates. it normally functions to provide the light-sensitive retinal cells with a second opportunity for photon-photoreceptor stimulation, thereby enhancing visual sensitivity at low light levels. the tapetum lucidum i ...200414738502
cutaneous leishmaniasis in red kangaroos: isolation and characterisation of the causative organisms.this is the first report of cutaneous leishmaniasis in kangaroos where infection was acquired within australia. the diagnosis is based on the clinical criteria used for humans, the lesion histopathology, the detection and isolation of parasites from the lesions, and the analysis of the small subunit ribosomal rna genes using the polymerase chain reaction. despite a clear indication that the parasites belong to the genus leishmania, no assignation to a known leishmania species could be made using ...200415111087
aerobic characteristics of red kangaroo skeletal muscles: is a high aerobic capacity matched by muscle mitochondrial and capillary morphology as in placental mammals?marsupials and placentals together comprise the theria, the advanced mammals, but they have had long independent evolutionary histories, with the last common ancestor occurring more than 125 million years ago. although in the past the marsupials were considered to be metabolically 'primitive', the red kangaroo macropus rufus has been reported to have an aerobic capacity (vo2max) comparable to that of the most 'athletic' of placentals such as dogs. however, kangaroos travel at moderate speeds wit ...200415235010
water use and the thermoregulatory behaviour of kangaroos in arid regions: insights into the colonisation of arid rangelands in australia by the eastern grey kangaroo (macropus giganteus).the eastern grey kangaroo (macropus giganteus) occurs mostly in the wetter regions of eastern australia. however, in the past 30-40 years it has moved into more arid regions (rainfall < 250 mm), thus increasing its overlap zone with the xeric adapted red kangaroo (macropus rufus). an increased access to water (supplied for domestic stock) may explain this range extension, but changes in the availability of preferred feed could also be involved. the water use, drinking patterns and thermoregulato ...200616208512
cryptosporidium fayeri n. sp. (apicomplexa: cryptosporidiidae) from the red kangaroo (macropus rufus).the morphology and infectivity of the oocysts of a new species of cryptosporidium from the faeces of the red kangaroo (macropus rufus) are described. oocysts are structurally indistinguishable from those of cryptosporidium parvum. oocysts of the new species are passed fully sporulated, lack sporocysts, and measure 4.5-5.1 microm (mean=4.9) x 3.8-5.0 microm (mean=4.3 microm) with a length to width ratio 1.02:1.18 (mean 1.14) (n=50). oocysts were not infectious for neonate arc swiss mice. multi-lo ...200818251799
prevalence of gastrointestinal bacterial pathogens in a population of zoo animals.faecal prevalence of gastrointestinal bacterial pathogens, including campylobacter, escherichia coli o157:h7, salmonella, shigella, yersinia, as well as arcobacter, were examined in 317 faecal specimens from 44 animal species in belfast zoological gardens, during july-september 2006. thermophilic campylobacters including campylobacter jejuni, campylobacter coli and campylobacter lari, were the most frequently isolated pathogens, where members of this genus were isolated from 11 animal species (1 ...200818331520
modelling digestive constraints in non-ruminant and ruminant foregut-fermenting mammals.it has been suggested that large foregut-fermenting marsupial herbivores, the kangaroos and their relatives, may be less constrained by food intake limitations as compared with ruminants, due mainly to differences in their digestive morphology and management of ingesta particles through the gut. in particular, as the quality of forage declines with increasing contents of plant fibre (cellulose, hemicelluloses and lignin; measured as neutral-detergent fibre, ndf), the tubiform foregut of kangaroo ...200818586113
seroepidemiology of toxoplasma gondii in zoo animals in selected zoos in the midwestern united states.toxoplasma gondii infections in zoo animals are of interest because many captive animals die of clinical toxoplasmosis and because of the potential risk of exposure of children and elderly to t. gondii oocysts excreted by cats in the zoos. seroprevalence of t. gondii antibodies in wild zoo felids, highly susceptible zoo species, and feral cats from 8 zoos of the midwestern united states was determined by using the modified agglutination test (mat). a titer of 1:25 was considered indicative of t. ...200818605803
australia's savanna herbivores: bioclimatic distributions and an assessment of the potential impact of regional climate change.the future impacts of climate change are predicted to significantly affect the survival of many species. recent studies indicate that even species that are relatively mobile and/or have large geographic ranges may be at risk of range contractions or extinction. an ecologically and evolutionary significant group of mammals that has been largely overlooked in this research is australia's large marsupial herbivores, the macropodids (kangaroos). the aims of our investigation were to define and compa ...200818937565
mandibular and maxillary osteomyelitis and myositis in a captive herd of red kangaroos (macropus rufus).jaw infections in macropods are common and will result in mortality if not promptly diagnosed and aggressively treated. they have most often been reported in wallabies; however, in the current case, the gross and histopathologic findings, microbial culture, and management of jaw infections in a population of red kangaroos (macropus rufus) housed in a zoological park are described. three red kangaroos, among a group of 23, were submitted for necropsy after death after progressively invasive and n ...200818987245
magnetic resonance imaging findings in a red kangaroo (macropus rufus) with otitis.magnetic resonance imaging (mri) was performed on an adult, male red kangaroo (macropus rufus) with a history of nonspecific neurologic signs and acute discharge from the left ear. mri revealed findings consistent with otitis and possible osteomyelitis of the temporal and mastoid bones. to the authors' knowledge, this is the first report of otitis and mri findings in a kangaroo.200819110716
new reports of australian cutaneous leishmaniasis in northern australian macropods.cutaneous leishmaniasis caused by various species of leishmania is a significant zoonotic disease in many parts of the world. we describe the first cases of australian cutaneous leishmaniasis in eight northern wallaroos, one black wallaroo and two agile wallabies from the northern territory of australia. diagnosis was made through a combination of gross appearance of lesions, cytology, histology, direct culture, serology and a species-specific real-time pcr. the causative organism was found to b ...200919288959
passage marker excretion in red kangaroo (macropus rufus), collared peccary (pecari tajacu) and colobine monkeys (colobus angolensis, c. polykomos, trachypithecus johnii).ruminants are characterized by an efficient particle-sorting mechanism in the forestomach (frst) followed by selective rechewing of large food particles. for the nonruminating foregut fermenter pygmy hippo it was demonstrated that large particles are excreted as fast as, or faster than, the small particles. the same has been suggested for other nonruminating foregut fermenters. we determined the mean retention time of fluids and different-sized particles in six red kangaroos (macropus rufus), se ...200919551808
toxoplasmosis and genotyping of toxoplasma gondii in macropus rufus and macropus giganteus in argentina.toxoplasma gondii infection is frequently asymptomatic; however, it can be severe or even fatal to some hosts. in this study, diagnosis of disseminated toxoplasmosis in one red kangaroo (macropus rufus) and one great grey kangaroo (macropus giganteus) from the la plata zoo, argentina and the isolation and molecular characterization of t. gondii are reported. both male kangaroos showed depression and sudden death. toxoplasma gondii infection was diagnosed by fresh examination, histopathology, imm ...201020045255
familiarity breeds contempt: kangaroos persistently avoid areas with experimentally deployed dingo scents.whether or not animals habituate to repeated exposure to predator scents may depend upon whether there are predators associated with the cues. understanding the contexts of habituation is theoretically important and has profound implication for the application of predator-based herbivore deterrents. we repeatedly exposed a mixed mob of macropod marsupials to olfactory scents (urine, feces) from a sympatric predator (canis lupus dingo), along with a control (water). if these predator cues were al ...201020463952
selected diagnostic ophthalmic tests in the red kangaroo (macropus rufus).the following tests were performed on a total of 20 eyes: schirmer tear test, intraocular pressure (iop), assessment of conjunctival flora, and pupillary diameter with application of topical tropicamide in 10 healthy captive red kangaroos (macropus rufus) under manual restraint. the mean schirmer tear test value was 22.6 +/- 6.07 mm/min. the mean intraocular pressure was 17.45 +/- 7.23 mm hg. values did not differ between eyes or gender for either test, but significant differences were identifie ...201020597213
evidence incriminating midges (diptera: ceratopogonidae) as potential vectors of leishmania in australia.the first autochthonous leishmania infection in australia was reported by rose et al. (2004) and the parasite was characterised as a unique species. the host was the red kangaroo (macropus rufus) but the transmitting vector was unknown. to incriminate the biological vector, insect trapping by a variety of methods was undertaken at two field sites of known leishmania transmission. collected sand flies were identified to species level and were screened for leishmania dna using a semi-quantitative ...201121251914
[muscle spindle distribution of masticatory and prevertebral muscles with special reference to histological properties of pouch and biological significance in red kangaroo, macropus rufus]. 20102956346
cellular degeneration in the testis of red kangaroos during hot weather and drought in central australia. 20154522372
steatitis in a red kangaroo (macropus rufus) associated with a coccidia-like protozoon. 19931554779
adrenal insufficiency in the red kangaroo (megaleia rufa, desm.). 20114411880
western australian marsupials are multiply infected with genetically diverse strains of toxoplasma gondii.five different organs from 16 asymptomatic free-ranging marsupial macropods (macropus rufus, m. fuliginosus, and m. robustus) from inland western australia were tested for infection with toxoplasma gondii by multi-locus pcr-dna sequencing. all macropods were infected with t. gondii, and 13 had parasite dna in at least 2 organs. in total, 45 distinct t. gondii genotypes were detected. fourteen of the 16 macropods were multiply infected with genetically distinct t. gondii genotypes that often part ...201223028812
infection with toxoplasma gondii in a red kangaroo (macropus rufus) and a patagonian mara (dolichotis patagonum) in captivity.toxoplasmosis is an infectious, zoonotic and parasitic disease, caused by toxoplasma gondii. in this manucript, two cases of infection with t. gondii in captive animals from a zoological park in the central region of chile are described. one case was a red kangaroo (macropus rufus), which is highly susceptible to the infection, and the other was a patagonian mara (dolichotis patagonum), a rodent in which there is no previous report of the infection. both animals had myocarditis, with the presenc ...201627982295
dermatophytosis in zoo macropods: a questionnaire study.limited published data are available on dermatophytosis in zoo macropods, despite anecdotal reports of disease occurrence and recurrent mob outbreaks. the aim of this questionnaire study was to analyze data from australian and international zoos to evaluate estimated disease prevalence in zoos housing macropods, affected macropod species, causative organisms, predisposing factors, clinical presentations, diagnostics, treatments, and disease risk management. two questionnaires (initial detailed a ...201324063082
high muscle mitochondrial volume and aerobic capacity in a small marsupial (sminthopsis crassicaudata) reveals flexible links between energy-use levels in mammals.we investigated the muscle structure-function relationships that underlie the aerobic capacity of an insectivorous, small (~15 g) marsupial, sminthopsis crassicaudata (family: dasyuridae), to obtain further insight into energy use patterns in marsupials relative to those in placentals, their sister clade within the theria (advanced mammals). disparate hopping marsupials (suborder macropodiformes), a kangaroo (macropus rufus) and a rat-kangaroo (bettongia penicillata), show aerobic capabilities a ...201323239895
forage fibre digestion, rates of feed passage and gut fill in juvenile and adult red kangaroos macropus rufus desmarest: why body size matters.using red kangaroos macropus rufus desmarest, a large (>20 kg) marsupial herbivore, we compared the digestive capabilities of juveniles with those of mature, non-lactating females on high-quality forage (chopped lucerne medicago sativa hay) of 43+/-1% neutral-detergent fibre (ndf) and poorer quality, high-fibre forage (chopped oaten avena sativa hay) of 64+/-1% ndf. on chopped lucerne apparent dry matter (dm) digestibilities by young-at-foot (yaf) red kangaroos (an age that would normally be tak ...200616574810
endogenous nitrogen excretion by red kangaroos (macropus rufus): effects of animal age and forage quality.red kangaroos (macropus rufus) are large (>20 kg) herbivorous marsupials common to arid and semiarid australia. the population dynamics of red kangaroos are linked with environmental factors, operating largely through juvenile survival. a crucial period is the young-at-foot (yaf) stage, when juveniles have permanently left the mother's pouch but still take milk from a teat in the pouch. forage quantity and quality have been implicated in drought-related mortalities of juvenile kangaroos. here we ...201316555200
shedding light on the microbial community of the macropod foregut using 454-amplicon pyrosequencing.twenty macropods from five locations in queensland, australia, grazing on a variety of native pastures were surveyed and the bacterial community of the foregut was examined using 454-amplicon pyrosequencing. specifically, the v3/v4 region of 16s rrna gene was examined. a total of 5040 otus were identified in the data set (post filtering). thirty-two otus were identified as 'shared' otus (i.e. present in all samples) belonging to either firmicutes or bacteroidetes (clostridiales/bacteroidales). t ...201323626688
comparative studies of water permeability of red blood cells from humans and over 30 animal species: an overview of 20 years of collaboration with philip kuchel.nmr measurements of the diffusional permeability of the human adult red blood cell (rbc) membrane to water (p(d)) and of the activation energy (e(a,d)) of the process furnished values of p(d) ~ 4 × 10(-3) cm/s at 25 °c and ~6.1 × 10(-3) cm/s at 37 °c, and e(a,d) ~ 26 kj/mol. comparative nmr measurements for other species showed: (1) monotremes (echidna and platypus), chicken, little penguin, and saltwater crocodile have the lowest p(d) values; (2) sheep, cow, and elephant have p(d) values lower ...201323104624
archaea in the foregut of macropod marsupials: pcr and amplicon sequence-based observations.to investigate, using culture-independent techniques, the presence and diversity of methanogenic archaea in the foregut of kangaroos.201222897363
effects of spines and thorns on australian arid zone herbivores of different body masses.we investigated the effects of thorns and spines on the feeding of 5 herbivore species in arid australia. the herbivores were the rabbit (oryctolagus cuniculus), euro kangaroo (macropus robustus), red kangaroo (macropus rufus), sheep (ovis aries), and cattle (bos taurus). five woody plants without spines or thorns and 6 woody plants with thorns were included in the study. the spines and thorns were not found to affect the herbivores' rates of feeding (items ingested/min), but they did reduce the ...199128312622
the feeding ecology of the dingo : iii. dietary relationships with widely fluctuating prey populations in arid australia: an hypothesis of alternation of predation.changes in the diet of dingoes (canis familiaris dingo) in response to measured fluctuations of prey populations were followed over 7 years. the study began after great rains had broken a long drought. eruptions of rodents and rabbits followed, but some prey were always either relatively abundant (live cattle) or scarce (red kangaroo, lizards, birds). cattle carcasses were increasingly available during a subsequent drought. small and medium-sized prey, rodents (26%), lizards (12%) and rabbits (5 ...198728311993
prolonged prey suppression by carnivores - predator-removal experiments.the hypothesis that carnivores can significantly suppress prey populations after they collapse during drought was tested by predator-removal experiments. low populations of rabbits (oryctolagus cuniculus) responded with significantly accelerated growth where foxes (vulpes vulpes) and feral cats (felis catus) were continually shot. experiments in years of good pasture and poor were confirmatory. after only 14 months, the rabbits were well on their way to another eruption whereas untreated populat ...198928312173
the laminar structure of the common opossum masseter (didelphis marsupialis).using three heads of the common opossum (didelphis marsupialis), which may be considered to have a primitive mammalian form and therefore be appropriate for this study, the laminar structure of the masseter was investigated. we also attempted a comparative anatomical study of the relationships of food habits to the laminar structures of the masseter, zygomatic arch and mandibular ramus. in the common opossum masseter, a total of six layers, the primary and secondary sublayers of the superficial ...200111392012
osteoarthritis in two marine mammals and 22 land mammals: learning from skeletal remains.the occurrence of osteoarthritis (oa) in marine mammals is still questionable. here we investigated the prevalence of oa in marine (dolphin and dugong) and terrestrial mammals (asian elephant, asiatic buffalo, camel, cat, cattle, deer, dog, domestic goat, horse, human, hyena, impala, lion, malayan tapir, assam macaque, mule, pig, rabbit, red kangaroo, sheep, tiger and waterbuck). skeletal remains obtained from five institutes were used as subjects; a total of 45 different parts (locations) of bo ...201728542897
molecular characterization and multi-locus genotypes of enterocytozoon bieneusi from captive red kangaroos (macropus rfus) in jiangsu province, china.enterocytozoon bieneusi is the most common pathogen of microsporidian species infecting humans worldwide. although e. bieneusi has been found in a variety of animal hosts, information on the presence of e. bieneusi in captive kangaroos in china is limited. the present study was aimed at determining the occurrence and genetic diversity of e. bieneusi in captive kangaroos. a total of 61 fecal specimens (38 from red kangaroos and 23 from grey kangaroos) were collected from nanjing hongshan forest z ...201728806735
energy requirements of the red kangaroo (macropus rufus): impacts of age, growth and body size in a large desert-dwelling herbivore.generally, young growing mammals have resting metabolic rates (rmrs) that are proportionally greater than those of adult animals. this is seen in the red kangaroo ( macropus rufus), a large (>20 kg) herbivorous marsupial common to arid and semi-arid inland australia. juvenile red kangaroos have rmrs 1.5-1.6 times those expected for adult marsupials of an equivalent body mass. when fed high-quality chopped lucerne hay, young-at-foot (yaf) kangaroos, which have permanently left the mother's pouch ...200312879349
subtype distribution of blastocystis isolates from a variety of animals from new south wales, australia.a total of 438 stool samples from 38 different species of animal from seven different locations were studied for the presence of blastocystis. pcr analysis was completed on all samples and dna sequence data from the rdna were submitted to subtype allocation. there was a total of 80 (18%) sequences from 18 species, and nine different subtypes were identified - st1, st2, st3, st4, st5, st7, st11, st12 and st13. this is the first report of blastocystis from the eastern grey kangaroo, red kangaroo, ...201323398989
molecular characterization of eimeria species in macropods.a total of 597 faecal samples were collected from western grey kangaroos (macropus fuliginosus), euros (m. robustus), red kangaroos (m. rufus) in western australia and eastern grey kangaroos (m. giganteus) from victoria and screened for the presence of eimeria by pcr at the 18s ribosomal rna (rrna) locus. the overall prevalence was 24.3% (145/597). at the 18s rrna locus, sequences were obtained for 25 of the 145 positives. phylogenetic analysis indicated that all the macropod-derived eimeria spe ...201222796750
the high aerobic capacity of a small, marsupial rat-kangaroo (bettongia penicillata) is matched by the mitochondrial and capillary morphology of its skeletal muscles.we examined the structure-function relationships that underlie the aerobic capacities of marsupial mammals that hop. marsupials have relatively low basal metabolic rates (bmr) and historically were seen as 'low energy' mammals. however, the red kangaroo, macropus rufus (family macropodidae), has aerobic capacities equivalent to athletic placentals. it has an extreme aerobic scope (fas) and its large locomotor muscles feature high mitochondrial and capillary volumes. m. rufus belongs to a modern ...201222660784
chemical characterization of milk oligosaccharides of the common wombat (vombatus ursinus).previous structural characterizations of marsupial milk oligosaccharides have been performed in the tammar wallaby, red kangaroo, koala, common brushtail possum and the eastern quoll. to clarify the homology and heterogeneity of milk oligosaccharides among marsupial species, which could provide information on their evolution, the oligosaccharides of wombat milk carbohydrate were characterized in this study. neutral and acidic oligosaccharides were isolated from the carbohydrate fractions of two ...201626608481
chemical characterization of milk oligosaccharides of the eastern quoll (dasyurus viverrinus).structural characterizations of marsupial milk oligosaccharides have been performed in four species to date: the tammar wallaby (macropus eugenii), the red kangaroo (macropus rufus), the koala (phascolarctos cinereus) and the common brushtail possum (trichosurus vulpecula). to clarify the homology and heterogeneity of milk oligosaccharides among marsupials, the oligosaccharides in the carbohydrate fraction of eastern quoll milk were characterized in this study. neutral and acidic oligosaccharide ...201526047593
seroprevalence of retrovirus in north american captive macropodidae.laboratory records of serology results from captive macropodidae sampled between 1997 and 2005 were reviewed to assess the seroprevalence of retrovirus exposure. serum samples from 269 individuals (136 males, 133 females) representing 10 species of macropods housed in 31 north american captive collections were analyzed for retrovirus antibody using an indirect immunofluorescent assay. the prevalence of positive antibody titers comparing male versus female, between species, between age groups, an ...200818816994
sry and karyotypic status of one abnormal and two intersexual marsupials.an intersexual agile wallaby (macropus agilis) with a penis, a pouch and four teats had a sex-chromosome constitution of xxy in lymphocytes and cultured fibroblasts; the sex-determining region y (sry) gene was present, consistent with the presence of a testis. an intersexual eastern grey kangaroo (macropus giganteus) with a small empty scrotum and no penis, and an abnormal red kangaroo (macropus rufus) with no penis, pouch or teats, both had xx sex-chromosome complements; the sry gene was not pr ...19979208434
host-parasite associations of eimeria spp. (apicomplexa:eimeriidae) in kangaroos and wallabies of the genus macropus (marsupialia:macropodidae).faecal samples from 514 kangaroos and wallabies representing 12 species of the genus macropus were examined for oocysts of eimeria spp. six species of eimeria were redescribed from their type hosts, and on the basis of finding homologous oocysts in the faeces of other macropus spp., host ranges for these coccidia were extended. eimeria hestermani mykytowycz, 1964 is redescribed from m. giganteus (eastern grey kangaroo) and is described from m. fuliginosus (western grey kangaroo), m. rufogriseus ...19892759765
repeated dna sequences and kangaroo phylogeny.three highly repeated dna sequences have been used to determine relationships of species within the macropodidae (kangaroos and wallabies). two highly repeated dna sequences were isolated as buoyant density satellites in the red-necked wallaby and in the wallaroo-euro group. the third probe was a cloned representative of one class of highly repeated species from the red kangaroo. radioactively labelled probes of these three repeated sequences were used to determine the incidence and distribution ...19817295212
chemical characterization of milk oligosaccharides of the common brushtail possum (trichosurus vulpecula).structural characterizations of marsupial milk oligosaccharides have been performed in only three species: the tammar wallaby, the red kangaroo and the koala. to clarify the homology and heterogeneity of milk oligosaccharides among marsupials, 21 oligosaccharides of the milk carbohydrate fraction of the common brushtail possum were characterized in this study. neutral and acidic oligosaccharides were separated from the carbohydrate fraction of mid-lactation milk and characterized by (1)h-nuclear ...201424906475
chemical characterization of milk oligosaccharides of the koala (phascolarctos cinereus).previous structural characterizations of marsupial milk oligosaccharides had been performed in only two macropod species, the tammar wallaby and the red kangaroo. to clarify the homology and heterogeneity of milk oligosaccharides among marsupial species, which could provide information on their evolution, the oligosaccharides of the koala milk carbohydrate fraction were characterized in this study. neutral and acidic oligosaccharides were separated from the carbohydrate fraction of milk of the k ...201323824565
chemical characterization of acidic oligosaccharides in milk of the red kangaroo (macropus rufus).in the milk of marsupials, oligosaccharides usually predominate over lactose during early to mid lactation. studies have shown that tammar wallaby milk contains a major series of neutral galactosyllactose oligosaccharides ranging in size from tri- to at least octasaccharides, as well as β(1-6) linked n-acetylglucosamine-containing oligosaccharides as a minor series. in this study, acidic oligosaccharides were purified from red kangaroo milk and characterized by (1)h-nuclear magnetic resonance sp ...201222415147
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