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hepatozoon species (apicomplexa: hepatozoidae) of the corn snake, elaphe guttata (serpentes: colubridae) and the pigmy rattlesnake, sistrurus miliarius barbouri (serpentes: viperidae) in south florida.hepatozoon guttata n. sp. is described from the corn snake (elaphe guttata guttata) from south florida. gamonts average 14.6 x 4.6 (13-17 x 3.5-6) and are not recurved, with nucleus situated in the second quarter of the gamont. erythrocyte cytoplasm rarely appears partially dehemoglobinized. the infected cells are usually distorted and are longer than the uninfected erythrocytes but do not differ in width; nuclei are smaller in length and width than those of uninfected cells. sporogony in aedes ...200212197130
proteomic analysis of ontogenetic and diet-related changes in venom composition of juvenile and adult dusky pigmy rattlesnakes (sistrurus miliarius barbouri).snake venom proteins show high levels of variation at the level of the individual yet the environmental and molecular mechanisms that generate this diversity remain unclear. here we report the results of a controlled feeding experiment combined with proteomic analyses of periodically collected venom samples to assess the roles of ontogenetic and diet-related effects on venom composition of captive juvenile and adult dusky pigmy rattlesnakes (sistrurus miliarius barbouri). juvenile snakes fed fro ...201121722760
an outbreak of fungal dermatitis and stomatitis in a free-ranging population of pigmy rattlesnakes (sistrurus miliarius barbouri) in florida.between september 1997 and march 1998, a severe skin, eye, and mouth disease was observed in a population of dusky pigmy rattlesnakes (sistrurus miliarius barbouri), at the lake woodruff national wildlife refuge in volusia county, florida (usa). three affected pigmy rattlesnakes were submitted for necropsy. all snakes had severe necrotizing and predominantly granulomatous dermatitis, stomatitis, and ophthalmitis, with involvement of the subadjacent musculature and other soft tissues. numerous fu ...200312910760
citrate is a major component of snake venoms.citrate has been identified as a major component of snake venoms by gas liquid chromatography and mass spectrometry. the venoms of bothrops asper, crotalus atrox, crotalus viridis viridis, crotalus adamanteus, sistrurus miliarius barbouri, crotalus horridus horridus, agkistrodon contortrix mokasen, agkistrodon contortrix contortrix and agkistrodon piscivorus piscivorus contain citrate at concentration levels which can serve as effective buffers. calcium, magnesium, zinc, iron, sodium and potassi ...19921626327
isolation, sequence analysis, and biological activity of atrolysin e/d, the non-rgd disintegrin domain from crotalus atrox venom.crotalid snake venom metalloproteinases often have associated with them nonproteinase domains that may be processed from the mature proteinases. nascent atrolysin e, from the western diamondback rattlesnake, crotalus atrox, has a metalloproteinasedomain and a non-rgd disintegrin domain that is lacking in the mature metalloproteinase. in this studywe report on the isolation, sequence analysis, andbiological activity of the 7.4-kda atrolysin e disintegrin domain (atrolysin e/d). atrolysin e/d repr ...19989637732
pygmy rattlesnake envenomation treated with crotalidae polyvalent immune fab antivenom.documented envenomations by the pygmy rattlesnake (sistrurus miliarius barbouri) are rare. while there have been no documented fatalities, several older case reports describe significant morbidity. we describe the first known case of pygmy rattlesnake envenomation that was treated with crotalidae polyvalent immune fab antivenom (crofab®).201222963729
snake venomics: characterization of protein families in sistrurus barbouri venom by cysteine mapping, n-terminal sequencing, and tandem mass spectrometry analysis.the protein composition of the crude venom of sistrurus barbouri was analyzed by two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. proteins were separated by reversed phase high-performance liquid chromatography and characterized by n-terminal sequence analysis. the molecular mass and number of cysteine residues of the purified proteins were determined by matrix-associated laser desorption/ionization-time of flight mass spectrometry. selected protein bands were subjected ...200414760702
venom proteomes of closely related sistrurus rattlesnakes with divergent diets.the protein composition of the venoms of the three subspecies of sistrurus catenatus (s. c. catenatus, tergeminus, and edwardsii) and a basal species, sistrurus miliarius barbouri, were analyzed by rp-hplc, n-terminal sequencing, maldi-tof peptide mass fingerprinting, and cid-ms/ms. the venoms of the four sistrurus taxa contain proteins from 11 families. the protein family profile and the relative abundance of each protein group in the different venoms are not conserved. myotoxins and 2-chain pl ...200616944921
proteomic profiling of a snake venom using high mass detection maldi-tof mass spectrometry.proteomic profiling involves identification and quantification of protein components in complex biological systems. most of the mass profiling studies performed with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) have been restricted to peptides and small proteins (<20 kda) because the sensitivity of the standard ion detectors decreases with increasing ion mass. here we perform a protein profiling study of the snake venom sistrurus miliarius barbouri, ...200717204429
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