| toxic fractions of cerastes cerastes venom. | | 1977 | 409869 |
| immunological properties of antivenins. i. bivalent cerastes cerastes and cerastes vipera antivenin. | | 1975 | 812369 |
| fractionation of the snake venoms of cerastes cerastes and cerastes vipera. | | 1977 | 854937 |
| effects of sand viper (cerastes cerastes) venom on isolated smooth muscle and heart and on haematological and cardiovascular parameters in the guinea-pig. | the effects of the venom of the sand viper (cerastes cerastes) on haematological and cardiovascular parameters and on isolated ileum, trachea, pulmonary artery and atrium from the guinea-pig were studied. in concentrations from 0.2 micrograms/ml to 0.6 mg/ml, snake venom caused concentration-dependent relaxation of the longitudinal ileal segments and the epinephrine-precontracted pulmonary artery rings, increased the tone of the tracheal rings and increased the rate of the spontaneously beating ... | 1992 | 1440630 |
| effect of venom from cerastes cerastes (egyptian sand viper) on luminol-dependent chemiluminescence of human blood phagocyte cells. | cerastes cerastes venom added in vitro to human whole blood caused a marked inhibitory effect on the luminol-dependent chemiluminescence induced by phorbol myristate acetate (pma) or opsonized zymosan on phagocyte cells. the inhibitory effect produced by the venom was both dose- and time-dependent when pma was used as the stimulant of the oxidative burst. similarly, the venom produced a significant inhibitory effect when added to isolated polymorphonuclear leukocytes (pmns). incubation of the is ... | 1990 | 2176186 |
| purification and characterization of phosphodiesterase (exonuclease) from cerastes cerastes (egyptian sand viper) venom. | a venom exonuclease 'phosphodiesterase' (e.c. 3.1.4.1) has been purified from cerastes cerastes venom by a combination of gel filtration on sephadex g-100 superfine and ion exchange chromatography on deae-sepharose. the enzyme showed a single band on page and sds-page and had a molecular weight of 110,000. the final preparation was purified 28 fold. it had no carbohydrate and it did not have protease or 5'-nucleotidase activities. optimum temperature for enzyme activity was 56 degrees c. the enz ... | 1987 | 2829390 |
| proteolysis in vitro of low and high density lipoproteins in human plasma by cerastes cerastes (egyptian sand viper) venom. | envenomation by snake venoms would be expected to result in proteolysis of plasma proteins as well as of cellular constituents. incubation of human serum with crude venom from cerastes cerastes showed that the plasma lipoproteins were a target of this venom. fractionation of the crude venom by gel filtration revealed that high density lipoprotein (hdl) was susceptible almost exclusively to the highest mol. wt fraction of venom and that proteolysis was due to a metalloprotease. although hdl was d ... | 1988 | 3144061 |
| coagulant component in cerastes cerastes (egyptian sand viper) venom. | a coagulant component has been purified from cerastes cerastes venom, using gel filtration on a sephadex g 100 (fine) column followed by chromatography on a bio-rex 70 column. this compound had a proteolytic effect and could coagulate human plasma deficient in factor viii or (viii + ix) with the formation of a firm clot. it could also clot plasma deficient in factor x, but the clot formed was soft and not complete. the compound had no effect on platelet aggregation and was nontoxic. this compoun ... | 1986 | 3564057 |
| effect of venom from bitis nasicornis (rhinoceros horned viper) on isolated rabbit aortic strips. | venom from b. nasicornis, but not from bitis arietans, echis carinatus or cerastes cerastes, produced an irreversible contraction of the isolated aortic strip that was slow in onset, increased with time, and reached maximum in about 10-15 min. the contraction was not inhibited by pretreatment with atropine, yohimbine, phentolamine, cyproheptadine or indomethacin, however, it was blocked by incubation in a ca2+-free solution and was partially blocked by incubation with the ca2+ channel blockers v ... | 1986 | 3715900 |
| protein c activators in snake venoms. | venoms of 32 snake species were tested for protein c (pc) activating potency. as measured with the chromogenic pc substrate d-pro-l-pro-l-arg-pna, eleven venoms were able to generate amidolytic activity from purified bovine pc. in five venom solutions (bothrops moojeni, b. pradoi, cerastes cerastes, vipera lebetina and v. russellii) the pc activating potency was destroyed during 10 min heating at 70 degrees c at ph 3, whereas in six venom solutions (agkistrodon contortrix contortrix, a. c. mokas ... | 1986 | 3755037 |
| [immunological study and hemolytic activity of the venom of the horned viper, cerastes cerastes (linne, 1758) (reptilia, viperidae)]. | the immunological study of the venom of the horned viper, cerastes cerastes (linné, 1758) points out eight fractions from which the components have various hemolyticus actions. | 1985 | 3937498 |
| effect of cerastes cerastes venom on blood and tissue histamine and on arterial blood pressure. | | 1969 | 4180507 |
| studies on egyptian cerastes cerastes antivenin. | | 1974 | 4218382 |
| effect of cerastes cerastes venom on blood coagulation mechanisms. | | 1969 | 5358062 |
| acid and alkaline phosphomonoesterases in egyptian snake venoms. | non-specific acid and alkaline phosphomonoesterases could be demonstrated in two viperids (cerastes cerastes and cerastes vipers) and two elapids (naja haje and naja nigricollis). the latter could be a natural source for the production of these enzymes. the activities of both enzymes in elapids were greater than in viperids. n. nigricollis was the only to show acid phosphatase activity exceeding its alkaline one. the optimum ph values recorded for acid phosphatase was 4.0 and 4.9 and for alkalin ... | 1981 | 6264698 |
| effect of the venom of the snake cerastes cerastes (african desert horned viper) on the response of the rat vas deferens to field stimulation. | cerastes cerastes venom in low concentrations (0.8-2.5 micrograms/ml) inhibited the response of the rat vas deferens to field stimulation, while higher concentrations (4-8 micrograms/ml) causes a contracture that was reversed by washing and transiently blocked by phentolamine. the effect of the venom and guanethidine (10(-7) m) was reversed by tyramine (4.6 x 10(-8) m) but unaffected by hexamethonium (1.4 x 10(-7) m). when the response to field stimulation was inhibited by the venom the response ... | 1982 | 6281942 |
| [infections and deaths of horned vipers, cerastes cerastes (l., 1758) and lebetin vipers, vipera lebetina (l., 1758) caused by pseudomonas aeruginosa (schröeter, 1885)]. | the authors present some data about the buccal and intestinal microflora of ophidian reptiles schooling in the vivaria of the institute pasteur of tunis. they describe two cases of infestation and dead, chiefly symptomatology, of a horned viper, cerastes cerastes (l. 1758) and a lebetin viper, vipera lebetina (l., 1758) by pseudomonas aeruginosa schröeter, 1885. | 1984 | 6443546 |
| growth inhibition of trypanosoma cruzi and leishmania donovani infantum by different snake venoms: preliminary identification of proteins from cerastes cerastes venom which interact with the parasites. | venom from three different snake species was tested in vitro against the protozoan parasites trypanosoma cruzi and leishmania donovani infantum. two of them, cerastes cerastes and naja haje, exerted a significant growth inhibition of t. cruzi and l. d. infantum parasites. heating of the venoms abolished their activity, suggesting that the active factors are thermolabile. incubation of parasites with 125i-labelled c. cerastes venom proteins allowed preliminary identification of components which i ... | 1994 | 7985193 |
| the effect of the viper cerastes cerastes cerastes venom and venom fractions on carbohydrate metabolism. | the effect of the viper cerastes cerastes cerastes venom and venom fractions on carbohydrate metabolism. toxicon 31, 791-801, 1993.--i.p. injection of a sublethal dose of cerastes cerastes cerastes venom into white rats induced a marked hypoglycemia after 15 min. this hypoglycemia continued for at least 24 hr. hypoglycemia was accompanied by a significant increase of liver and muscle glycogen at 15-30 min and 8 hr. plasma lactate levels were significantly increased for most of the 24 hr test per ... | 1993 | 8342177 |
| afaâcytin, an alpha beta-fibrinogenase from cerastes cerastes (horned viper) venom, activates purified factor x and induces serotonin release from human blood platelets. | afaâcytin, a proteinase with caseinolytic, arginine-esterase and amidase activities, was purified from the venom of cerastes cerastes (horned viper) in two steps by gel filtration through sephadex g75, then hplc on carboxymethyl-cellulose. afaâcytin has an isoelectric point of 6.25, and consists of two subunits, alpha and beta, which have the same apparent molecular mass (40,000) and are indistinguishable in the absence of reduction or/and deglycosylation. subunit beta is constituted of two disu ... | 1995 | 8521839 |
| cerastatin, a new potent inhibitor of platelet aggregation from the venom of the tunisian viper, cerastes cerastes. | cerastatin, a potent platelet aggregation inhibitor, was purified by gel filtration on sephadex g-75, followed by two ion exchange chromatographies on mono-s columns. cerastatin is a neutral glycoprotein (pi = 6.2) of 32 kda, made up of at least three subunits. it is devoid of phospholipase a2, esterase, fibrinogenolytic and amidolytic activities. it inhibits aggregation of washed platelets, induced by either collagen, paf acether or thrombin, with similar ic50 of 2.3 nm. cerastatin also inhibit ... | 1997 | 9028015 |
| procoagulant and platelet-aggregating properties of cerastocytin from cerastes cerastes venom. | cerastocytin is a thrombin-like serine protease with potent platelet-proaggregating properties. it is able to activate factor xiii but is less active than thrombin on plasma coagulation. the aggregation induced by cerastocytin resembles that induced by thrombin, since rabbit washed platelets desensitized by a pretreatment with thrombin do not aggregate in the presence of cerastocytin. furthermore, preincubation of platelets with monoclonal antibodies specific for glycoproteins gpib or gpiibiiia ... | 1997 | 9080583 |
| cerastotin, a serine protease from cerastes cerastes venom, with platelet-aggregating and agglutinating properties. | cerastotin, a thrombin-like enzyme from the venom of the desert viper cerastes cerastes, has been purified by gel filtration on sephadex g-75 and two ion-exchange chromatographies on mono s columns. it is a neutral glycoprotein (pi = 6.6), present as a single polypeptide chain of 40 kda. its n-terminal sequence shows strong similarity with those of other thrombin-like enzymes from snake venoms. cerastotin possesses esterase and amidolytic activities measured with n(alpha)-tosyl-l-arginine methyl ... | 1997 | 9249017 |
| prothrombin and factor x activator activities in the venoms of viperidae snakes. | a ca(2+)-dependent prothrombin activator, carinactivase-1 (ca-1), was previously found in the venom of echis carinatus leucogaster. in the present study, the activities of ca-1-like enzymes were screened in the venoms of various viperidae snakes. the addition of 1 mm ca2+ ions to the venoms of only echis snakes in viperidae produced considerably high prothrombin activator activity, indicating that only the echis snake venoms contain not only the ca(2+)-independent prothrombin activator, ecarin, ... | 1997 | 9428105 |
| ultrastructural changes in the alveolar cells of rats injected with cerastes cerastes cerastes venom. | ultrastructural changes in the alveolar tissue of rats intraperitoneally injected with the cerastes cerastes cerastes venom were studied in 2 different experimental groups. in the first group, each rat was given 0.73 mg/kg as a single dose and sacrificed after 24 hours. in the second group, each rat was given a daily dose of 0.42 mg/kg for 7 days and sacrificed 24 hours after the last injection. proliferative changes were seen in type ii alveolar cells, fibroblasts, lymphocytes, and macrophages. ... | 1997 | 9496380 |
| purification of a 5-ht uptake inhibitor from the venom of cerastes vipera. | a protein that inhibits the re-uptake of 5-hydroxytryptamine into rat brain synaptosomes was isolated from the venom of the sahara sand viper (cerastes vipera) by gel filtration and reverse phase chromatography. it has a molecular weight of 13,739 da and an ic50 of about 50 nm for blocking uptake of 3h-5-ht into rat brain synaptosomes. it also augmented the responses to 5-ht in a smooth muscle preparation. it has phospholipase a2 activity, but it has no lytic activity as measured by its inabilit ... | 1998 | 9643472 |
| renal alterations induced by cerastes cerastes cerastes venom. | the purpose of the present study was to determine the structural and functional alterations in the kidneys of rats experimentally envenomated with the viper cerastes cerastes cerastes. the effects of a single i.p. sub-ld50 (0.73 mg kg-1) for 24 hr and 7 consecutive doses (0.42 mg kg-1; daily injection) on the kidneys of rats were studied. histological examination of the kidneys in envenomated rats revealed the presence of atrophied glomeruli, cloudy materials within the proximal and distal convo ... | 1998 | 9689602 |
| effect of some variables on the in vivo determination of scorpion and viper venom toxicities. | an adequate assessment of scorpion and snake venom ld50 is an important step for accurate evaluation of antivenom sera potencies and the optimization of serotherapy. the ld50 variation of tunisian scorpion (androctonus australis garzonii: aag and buthus occitanus tunetanus: bot) venoms with body weight, sex and strain (swiss or c57bi/6) of mice used, the route of venom injection, the venom-milking procedures (manually or electrically) and the venom batches have been studied over a 7-year period ... | 1998 | 10403031 |
| venomous snakes of israel: ecology and snakebite. | ten species of venomous snakes belonging to three families occur in israel and in jordan, some of which may pose a serious threat to humans. specific, local antivenins are available against only two of the species, while against others regional or european preparations are used. it is suggested that in addition to the monospecific anti-vipera palaestinae, a polyspecific antivenin be prepared against the clinically most important venomous snakes of the region, namely, echis coloratus, pseudoceras ... | 1998 | 10444959 |
| the dielectric properties of neutron irradiated snake venom and its pathological impact. | the changes in the dielectric properties of a saline solution of cerastes cerastes snake venom after irradiation with low-level doses of fast neutrons from a cf-252 source, were investigated. the pathological changes in the internal organs such as liver, kidney spleen, lung and heart of the rats injected with unirradiated and irradiated venom were also studied. the changes in the molecular structure of a diluted saline solution of snake venom were measured through dielectric relaxation studies i ... | 1999 | 10495125 |
| molecular cloning and expression of a functional snake venom serine proteinase, with platelet aggregating activity, from the cerastes cerastes viper. | the venoms of viperidae snakes contain numerous serine proteinases that have been recognized to possess one or more of the essential activities of thrombin on fibrinogen and platelets. among them, a platelet proaggregant protein, cerastocytin, has been isolated from the venom of the tunisian viper cerastes cerastes. using the race-pcr technique, we isolated and identified the complete nucleotide sequence of a cdna serine proteinase precursor. the recombinant protein was designated rcc-ppp (for c ... | 2003 | 12962484 |
| evaluation of the effect of gamma rays on the venom of vipera lebetina by biochemical study. | snake bites represent a serious public health problem in many areas of the world. in algeria, two widespread snakes are vipera lebetina and cerastes cerastes. vipera lebetina venom causes local hemorrhage and necrosis, and it may lead to permanent limb loss. the principal causes of mortality after snakebites are acute renal failure and hemorrhage, which occur not only locally, at the site of the bite, but also systemically, contributing to the cardiovascular shock characteristic of severe enveno ... | 2003 | 14719029 |
| [thrombin-like serine proteases in cerasted venoms (cerasted cerastes and cerastes vipera)]. | cerastes cerastes and cerastes vipera snake venoms are rich of thrombin-like, serine-protease polypeptides. many proteins have been isolated, purified and characterized from these vipers. these proteins act in various way on blood coagulation pathway and platelet function. | 1998 | 14722941 |
| [effect of macrovipera lebetina and cerastes cerastes venoms on adherence to integrins of cancerous cells (igr39, ht29-d4 and igrov1)]. | in this work, we provide experimental arguments in favor of the fact that components from macrovipera lebetina and cerastes cerastes venoms bind to igr39 melanoma cells but not to ht29d4 cells that derive from carcinoma adenome. furthermore, macrovipera lebetina and cerastes cerastes venoms inhibit the adherence of igr39 and ht 29-d4 to various extracellular matrix proteins. macrovipera lebetina and cerastes cerastes venoms did not inhibit the non specific adherence of igr 39 cells to polylysine ... | 2002 | 15072239 |
| life-threatening envenoming by the saharan horned viper (cerastes cerastes) causing micro-angiopathic haemolysis, coagulopathy and acute renal failure: clinical cases and review. | the desert horned vipers (cerastes cerastes and c. gasperettii) are the most familiar snakes of the great deserts of north africa and the middle east, including the plains of iraq. they are responsible for many human snake bites. in western countries, they are popular among exotic-snake keepers. | 2004 | 15496528 |
| bioinformatics and multiepitope dna immunization to design rational snake antivenom. | snake venom is a potentially lethal and complex mixture of hundreds of functionally diverse proteins that are difficult to purify and hence difficult to characterize. these difficulties have inhibited the development of toxin-targeted therapy, and conventional antivenom is still generated from the sera of horses or sheep immunized with whole venom. although life-saving, antivenoms contain an immunoglobulin pool of unknown antigen specificity and known redundancy, which necessitates the delivery ... | 2006 | 16737347 |
| the effect of crude cerastes cerastes gasperetti venom on the activity of key metabolic enzymes in cultured human fibroblasts. | cultured human fibroblasts were used to study the effect of a crude extract of cerastes cerastes gasperetti venom on the activity of a profile of key enzymes of metabolism. a single concentration of the crude venom was incubated with confluent fibroblasts established from six normal subjects for a period of three hours. a dramatic reduction in the specific activities of glucose and glycogen degradative enzymes was observed (23.7 +/- 3.9%, 36.3 +/- 8.7% and 71.1 +/- 5.7% of control for citrate sy ... | 1992 | 17589155 |
| irradiated cerastes cerastes venom as a novel tool for immunotherapy. | immunotherapy is the most effective treatment for the snake bites. the antivenoms are commonly obtained by hyperimmunization of animals that suffer from venom toxicity. the present report describes gamma irradiation effects on cerastes cerastes venom. doses of 1 kgy and 2 kgy gamma radiations were used for venom detoxification. these treated venoms did not have any residual lethal effects until 10 ld(50). immunological analysis of sera raised against native and irradiated venoms, showed that eli ... | 2008 | 18306103 |
| snake venom inhibits the growth of mouse mammary tumor cells in vitro and in vivo. | crude preparations as well as purified components of venoms isolated from american, asian and european snakes have been shown to inhibit the growth of some mouse and human cancer cell lines. however, it is not known if cerastes cerastes venom (ccv) obtained from the egyptian desert possesses any anti-tumor activity. in the present study we examined in vitro the effect of ccv on the growth rate and morphology of a mouse mammary tumor virus-induced mammary tumor cell line (riii/sa-mt). in addition ... | 2009 | 19327376 |
| two purified and characterized phospholipases a2 from cerastes cerastes venom, that inhibit cancerous cell adhesion and migration. | two non-toxic pla2s were purified to homogeneity from cerastes cerastes tunisian snake venom. the purification process employed gel filtration on sephadex g-75 followed by c18 reverse phase high-pressure liquid chromatography. these two acidic enzymes, namely cc-pla2-1 and cc-pla2-2, have a molecular weight of 13,737.52 and 13,705.63 da, respectively. these two pla2 are the first reported glycosylated phospholipases a2 purified from snake venom. the rates of glycosylation are 2.5% and 0.5% (w/w) ... | 2009 | 19708222 |
| increased antioxidant potential and decreased free radical production in response to mild injection of crude venom, cerastes cerastes gasperetti. | abstract the purpose of this study was to examine the effects of cerastes cerastes gasperetti crude venom (ccv) on free radical (fr) production and biological antioxidant potential (bap). thirty male rats, matched with age and weight, were divided randomly into three groups: control (c), negative control (nc), and experimental (e). animals in the control group served as control for needlestick and venom injection. animals in the negative control group, which served as control for venom-induced e ... | 2008 | 20020886 |
| cc-pla2-1 and cc-pla2-2, two cerastes cerastes venom-derived phospholipases a2, inhibit angiogenesis both in vitro and in vivo. | integrins are essential in the complex multistep process of angiogenesis and are thus attractive targets for the development of antiangiogenic therapies. integrins are antagonized by disintegrins and c-type lectin-like proteins, two protein families from snake venom. here, we report that cc-pla2-1 and cc-pla2-2, two novel secreted phospholipases a(2) (pla(2)) isolated from cerastes cerastes venom, also showed anti-integrin activity. indeed, both pla(2)s efficiently inhibited human brain microvas ... | 2010 | 20142800 |
| acute pancreatitis after viperid snake cerastes cerastes envenoming: a case report. | snakebites by the viper snake cerastes cerastes are quite common across the world but rarely published with regard to their mostly benign course. the reported case study depicts the envenoming of a 36-year-old czech man, a private herpetologist, who suffered snakebite to his finger. he developed a painful local reaction with hemorrhagic oedema. subsequently, consumption coagulopathy with thrombocytopenia and haemolysis, colicky pain in the epigastrium and acute renal failure developed. acute exu ... | 2010 | 20359440 |
| [an unusual mode of snake poisoning in tunisia]. | snake poisoning constitutes a real public health problem in the north african countries. in tunisia, snake poisonings are particularly frequent in the sub-saharan regions (southern areas) with high mortality and possible debilitation in survivors. the most common mode of snake poisoning is biting, particularly on the extremities. the purpose of this report is to describe a case involving a patient who presented authentic systemic signs of a snake poisoning after ingesting a whole raw viper of th ... | 2010 | 20486364 |
| ccsv-mpase, a novel procoagulant metalloproteinase from cerastes cerastes venom: purification, biochemical characterization and protein identification. | a procoagulant metalloproteinase called ccsv-mpase was purified from c. cerastes venom by successive chromatographic methods starting with gel-filtration through sephadex g-75; ion-exchange deae-cellulose a-50; affinity chromatography on benzamidine sepharose 6b and rp-hplc on a c8 column. ccsv-mpase has been isolated to an extent of about tenfolds and its molecular mass was evaluated at 70 kda by sds-page. ccsv-mpase hydrolyzes casein and fibrinogene as natural substrates. its proteolytic activ ... | 2010 | 20717713 |
| assessment of the antimicrobial activity of few saudi arabian snake venoms. | venoms of two cobras, four vipers, a standard antibiotic and an antimycotic, were evaluated comparatively, as antimicrobials. | 2015 | 26668657 |
| purification, characterization and antibacterial activity of l-amino acid oxidase from cerastes cerastes. | antibiotic resistance presents a real problem in which new antibacterial molecules from natural secretions could be beneficial in the development of new drugs. in this study, cerastes cerastes venom was investigated for its antibacterial activity against gram-positive and gram-negative bacteria. the antibacterial activity was evaluated by measuring the halo inhibition and minimum inhibitory concentration (mic). an l-amino acid oxidase (cclaao) was purified from this venom using three chromatogra ... | 2014 | 24817275 |
| in vitro antischistosomal activity of venom from the egyptian snake cerastes cerastes. | we studied the potential in vitro antischistosomal activity of cerastes cerastes venom on adult schistosoma mansoni worms. | 2017 | 28001223 |
| identification of cdnas encoding viper venom hyaluronidases: cross-generic sequence conservation of full-length and unusually short variant transcripts. | the immobilisation of prey by snakes is most efficiently achieved by the rapid dissemination of venom from its site of injection into the blood stream. hyaluronidase is a common component of snake venoms and has been termed the "venom spreading factor". in the absence of nucleotide or protein sequence data to confirm the functional identity of this venom component, we interrogated a venom gland est database for the saw-scaled viper, echis ocellatus (nigeria), using the gene ontology (go) term "c ... | 2007 | 17210232 |
| snakebites notified to the poison control center of morocco between 2009 and 2013. | snakebites cause considerable death and injury throughout the globe, particularly in tropical regions, and pose an important yet neglected threat to public health. in 2008, the centre anti poison et de parmacovigilance du maroc (capm) started to set up a specific strategy for the control of snakebites that was formalized in 2012. the aim of the present study is to describe and update the epidemiological characteristics of snakebites notified to capm between 2009 and 2013. | 2016 | 26985186 |
| evaluation of the lethal potency of scorpion and snake venoms and comparison between intraperitoneal and intravenous injection routes. | scorpion stings and snake bites are major health hazards that lead to suffering of victims and high mortality. thousands of injuries associated with such stings and bites of venomous animals occur every year worldwide. in north africa, more than 100,000 scorpion stings and snake bites are reported annually. an appropriate determination of the 50% lethal doses (ld₅₀) of scorpion and snake venoms appears to be an important step to assess (and compare) venom toxic activity. such ld₅₀ values are als ... | 2014 | 24926799 |
| antigenic relationship between the venom of the night adder causus maculatus and venoms of other viperids. | monovalent antivenoms were raised in mice against the venoms of causus maculatus, vipera ammodytes, echis carinatus, cerastes cerastes, bitis arietans, agkistrodon rhodostoma and bothrops atrox. these antivenoms as well as four commercially available antivenoms were tested against the venoms of 15 viperid species by means of immunoelectrophoresis and/or elisa. cross-reactive protein bands were determined by immunoblot. elisa cross-reactions of c. maculatus antivenom were low with all heterologou ... | 1990 | 1706897 |
| developmental stages of hepatozoon seurati (laveran and pettit 1911) comb. nov., a parasite of the corned viper cerastes cerastes and the mosquito culex pipiens from egypt. | developmental stages of hepatozoon seurati (laveran and pettit 1911) comb. nov. are described from the tissues of the corned viper cerastes cerastes, and from the vector culex pipiens. the parasite described in the present study is firstly recorded as haemogregarina seurati (laveran and pettit 1911) in the same host. after demonstration of the sporogonous development in the mosquito vector (c. pipiens) which showed all characteristics of the genus hepatozoon (large oocysts containing many sporoc ... | 2013 | 23620221 |
| laboratory studies on the possibility of culex quinquefasciatus to harbor hepatozoon sp. infecting cerastes cerastes cerastes viper in egypt. | a successful experimental infection of culex quinquefasciatus with hepatozoon sp. infecting cerastes cerastes cerastes viper was carried out under laboratory conditions of 24 +/- 3 degrees c and 60-70% r. h. the period monitored for complete sporogonic cycle was 21 days. the effect of high parasitimic blood meal was nonsignificant (p>0.05) on preoviposition period and hatchability. meanwhile a highly significant reduction was observed in oviposition rate, number of deposited eggs, number of hatc ... | 2015 | 26939229 |
| light and electron microscopic study on a haemogregarine species infecting the viper cerastes cerastes gasperitti from saudi arabia. | a haemogregarine sp. infecting the viper cerastes cerastes gasperitti (35%) from k.s.a. was recorded for the 1st time. parasites were recorded exclusively in the red blood cells of the vipers. erythrocytic parasites measured 17.5 x 3 microm with a nucleus of 8.1 x 2.5 microm. mature schizonts of 30 x 2.5 microm and producing 18-30 merozoites by ectomerogony were reported in the endothelial cells of blood capillaries of different internal organs (liver, spleen and lung). the merozoites measured 1 ... | 2008 | 18817240 |
| [three case-reports of viperin envenoming in morocco]. | snake bites are responsible for a high mortality rate in africa. problems for the early care of the victims are many. we published three observations of moroccan typical viperin severe envenomings (rapidly extensive edema, necrosis, haemorrhagic shock) are presented. the overall mortality of those bites is 4%. in the maghreb, viperin syndromes are the result of the lebetin viper (vipera lebetina), the horned viper or sand viper (cerastes cerastes), sometimes bitis or echis sp. immunotherapy rema ... | 2008 | 18424055 |
| eimeria gasperettii n. sp. (apicomplexa: eimeriidae) from the horned viper, cerates cerastes gasperettii in saudi arabia. | eimeria gasperettii n. sp. is described from the gall bladder of the horned viper, cerastes cerastes gasperettii from al-thumamah, central region of saudi arabia. sporulated oocysts are ellipsoid in shape, measuring 38.8 x 25.5 (29-41 x 17.2-28.4) microm with smooth brownish-yellow bilayered wall, 1.7(1.5-2.0) microm.thick. micropyle polar granule and oocyst residuum are absent. sporocysts are ellipsoid reaching 13.8 x 9.6 (11-14.5 x 8.0-10.3) microm. sporocyst residuum is present. the sporocyst ... | 2003 | 14708848 |
| predator facilitation or interference: a game of vipers and owls. | in predator-prey foraging games, the prey's reaction to one type of predator may either facilitate or hinder the success of another predator. we ask, do different predator species affect each other's patch selection? if the predators facilitate each other, they should prefer to hunt in the same patch; if they interfere, they should prefer to hunt alone. we performed an experiment in a large outdoor vivarium where we presented barn owls (tyto alba) with a choice of hunting greater egyptian gerbil ... | 2014 | 24481981 |
| protective efficacy of immunoglobulins y prepared against cerastes cerastes snake venom in the kingdom of saudi arabia. | to prepare and evaluate the protective efficacy of immunoglobulin y (igy) prepared against local saudi cerastes cerastes snake venom. | 2012 | 22886116 |
| a fibrinogen-clotting serine proteinase from cerastes cerastes (horned viper) venom with arginine-esterase and amidase activities. purification, characterization and kinetic parameter determination. | an enzyme displaying proteolytic activity toward the natural substrate casein as well as clotting activity on fibrinogen was purified to homogeneity from cerastes cerastes (horned viper) venom and characterized. the enzyme is constituted of two identical subunits of mol. wt 48,500 as determined by sds-polyacrylamide gel electrophoresis, and has an isoelectric point of 3.75. n-terminal sequencing up to the 33rd residue evidenced a high homology with other snake venom proteinases. the proteinase i ... | 1992 | 1485336 |
| characterization of phospholipase a2 from the venom of horned viper (cerastes cerastes). | phospholipase a2 has been purified from the venom of horned viper (cerastes cerastes) by gel permeation chromatography followed by reverse-phase hplc. the primary structure was established by sequence analysis of the intact protein and its enzymic peptides. the structure has 120 residues, properties like other group iib phospholipases, but only 45-55% identity with the enzyme from other viperid species, and large variations even within the species (26% residue differences at known positions in a ... | 1991 | 1993470 |
| purification and properties of an activating enzyme of blood clotting factor x from the venom of cerastes cerastes. | an activator of blood coagulation factor x was found in the venom of the horned viper cerastes cerastes, and was purified by gel filtration, ion-exchange chromatography and chromatofocussing. the activator is a protein composed of a heavy and a light polypeptide chain linked by disulfide bonds. two subforms of the activator were found. both contained a heavy chain of mr 58000 and are distinguished from each other by the presence of two different light chains of mr 17700 and 15000. the activator ... | 1983 | 6603869 |
| isolation and characterization of an anti-leishmanial disintegrin from cerastes cerastes venom. | investigating new antimicrobial and antiparasitic components from viperidae venoms represents an alternative therapeutic strategy. in this study, we report the characterization of a disintegrin isolated from cerastes cerastes venom, exhibiting antiparasitic activity on leishmania infantum promastigotes. indeed, isolated disintegrin, referred to disintegrin_cc, induced 84.75% of parasiticidal activity and deep morphological alterations on the parasites. sds-page analysis indicated that this disin ... | 2018 | 29278277 |
| cerastes cerastes envenomation in an 18 year old female: a case report. | an 18 year old woman was bitten on the second finger of her right hand by a cerastes cerastes. a literature search revealed very few clinical cases, and most of those were only laboratory and in 'vitro studies'. local signs included a hemorrhagic wound at the site of the bite with marked swelling of the entire hand. twelve hours later, the patient developed coagulopathy, which lasted 3 days and required repeated administration of blood products. treatment was essentially supportive and the patie ... | 2002 | 12165327 |
| the use of ground-borne vibrations for prey localization in the saharan sand vipers (cerastes). | sand vipers of the genus cerastes are specialized semi-fossorial snakes that launch predatory strikes at rodents and lizards while partially buried in the soft sand of the saharan desert. this study attempted to document which environmental stimuli are used by these snakes as a trigger for the ambush behavior. denervating the olfactory and vomeronasal organs produced no changes in prey capture behavior in cerastes cerastes. occluding the eyes of the denervated specimens resulted in significant d ... | 2002 | 11907055 |
| the presence of the wgd motif in cc8 heterodimeric disintegrin increases its inhibitory effect on alphaii(b)beta3, alpha(v)beta3, and alpha5beta1 integrins. | two highly homologous dimeric disintegrins, cc5 and cc8, have been isolated from the venom of the north african sand viper cerastes cerastes. cc5 is a homodimer containing an rgd motif in its subunits. cc8 is a heterodimer. the cc8a and cc8b subunits contain rgd and wgd tripeptide sequence in their respective integrin-binding loops. both cc5 and cc8 inhibited platelet aggregation and the adhesion of cells expressing integrins alphaii(b)beta3, alpha(v)beta3, and alpha5beta1 to appropriate ligands ... | 2002 | 11827548 |
| characterization of a potent platelet aggregation inducer from cerastes cerastes (egyptian sand viper) venom. | a potent, proteinaceous inducer of platelet aggregation designated as iva, has been purified to homogeneity from cerastes cerastes venom by molecular sieve and ion exchange chromatography. it is composed of 2 subunits with total m(r) of 62,000 as shown by native gel chromatography and chemical cross-linking with disuccinimidyl suberate. it is not clear at the present time whether both subunits are identical gene products, however, both have identical n-terminal sequences for the first 15 amino a ... | 1995 | 7612660 |
| cerastocytin, a new thrombin-like platelet activator from the venom of the tunisian viper cerastes cerastes. | cerastocytin, a thrombin-like enzyme from the venom of the desert viper, cerastes cerastes, has been purified to homogeneity by fast performance liquid chromatography (fplc) on mono-q and mono-s columns. it is a basic protein (isoelectric point higher than 9) made of a single polypeptide chain of 38 kda. its n-terminal polypeptide sequence shows strong similarities with other thrombin-like enzymes from snake venoms. nanomolar concentrations of cerastocytin induce aggregation of blood platelets. ... | 1995 | 7766651 |
| purification and characterization of a phospholipase a2 from cerastes cerastes (horn viper) snake venom. | a single phospholipase a2 has been found in cerastes cerastes venom, purified to homogeneity by a combination of chromatographic steps involving gel filtration on sephadex g-50 and ion-exchange chromatography on deae-sephadex a-50. its mol. wt, its amino acid composition and its partial amino acid sequence have been determined. high homologies between its sequence and those of other viperid phospholipides a2 have been noticed. the phospholipase was non-lethal to mice up to a dose as high as 25 m ... | 1990 | 2402760 |
| cerastes cerastes (egyptian sand viper) venom induced platelet aggregation as compared to other agonists. | the egyptian sand viper (cerastes cerastes) crude venom and subfractions were, for the first time, shown to induce platelet aggregation with agonist activities present in two subfractions. the combined activities of the crude venom components behaved in a unique fashion as compared to the platelet agonists, adp, collagen and thrombin. the action of the venom was inhibited by conditions that increased camp, partially required the formation of thromboxane a2 and was inhibited by the serine proteas ... | 1988 | 2829899 |
| [identification of toxic components from the venom of cerastes cerastes and vipera lebetina vipers; purification of phospholipases]. | cerastes cerastes and vipera lebetina venoms have been fractionated and the different components analysed by electrophoresis on polyacrylamid gels. phospholipases a2 contained in these two venoms have been purified and their electrophoretic properties compared. | 2016 | 2845874 |
| further characterization of the anticoagulant proteinase, cerastase f-4 from cerastes cerastes (egyptian sand viper) venom. | a potent anticoagulant, cerastase f-4, was purified from the venom of cerastes cerastes. the u.v. absorption spectrum revealed a relatively high tyrosine and low tryptophan content. the molar extension coefficient and e278(0.1%) were 19,400 and 0.84, respectively. the enzyme secondary structure, as studied by circular dichroism, showed 23.6% alpha-helix, 34% beta-sheets, 19% beta-turns and 32.5% random coils. when casein was used as a substrate the optimum ph was 10.0 and the km was 1.45 g/l. ce ... | 1987 | 3118514 |
| isolation and characterization of an anticoagulant proteinase, cerastase f-4, from cerastes cerastes (egyptian sand viper) venom. | an anticoagulant protease, cerastase f-4, was isolated from the venom of cerastes cerastes (egyptian sand viper) by a combination of gel filtration, ion-exchange chromatography, and hplc. homogeneity of the purified anticoagulant was established by discontinuous polyacrylamide disc gel electrophoresis and by isotachophoresis. the anticoagulant enzyme is a single polypeptide chain without subunits having a molecular weight of 22,500. it consists of 28% aspartic acid residues and only 7% are basic ... | 1986 | 3010494 |
| mechanism of the anticoagulant, cerastase f-4, isolated from cerastes cerastes (egyptian sand viper) venom. | an anticoagulant enzyme, cerastase f-4, from the venom of cerastes cerastes was purified to homogeneity and was characterized (1). in the present report the mode of its fibrinogenolytic and fibrinolytic actions, and its effects on some other blood coagulation factors are described. cerastes f-4 was shown to readily hydrolyze the alpha a chain of fibrinogen followed by the hydrolysis of the beta b chain. the gamma-chain was relatively resistant to hydrolysis. it also degrades the three chains of ... | 1986 | 2939587 |
| [anatomo-pathological study of fatal cases of malpolon monspessulana (herman, 1809) (reptilia, colubridae) bitten in the head by cerastes cerastes (l., 1758) (reptilia, viperidae)]. | two malpolon monspessulana (herman, 1809) were bitten in the head by cerastes cerastes (l., 1758). the first specimen is fastly dead ten minutes after the bite. this short time does not permit us to point out the lesions by current technics, but only by autopsy two sting marks of c. cerastes poison fangs bites. the second specimen is dead eight hours after the bite, an anatomopathological study shows oedematous and haemorrhagico lesions of the conjunctive and striated muscular tissues. the visce ... | 1984 | 6544592 |
| effects of cerastes cerastes (egyptian sand viper) and cerastes vipera (sahara sand viper) snake venoms on blood coagulation: separation of coagulant and anticoagulant factors and their correlation with arginine esterase and protease activities. | | 1981 | 7013157 |
| proteases of cerastes cerastes (egyptian sand viper) and cerastes vipera (sahara sand viper) snake venoms. | | 1981 | 6784280 |
| fractionation of cerastes cerastes and cerastes vipera snake venoms by gel filtration and identification of some enzymatic and biological activities. | | 1979 | 227146 |
| [effect of cerastes cerastes snake bite on coagulation]. | | 1978 | 689516 |
| effect of the venom of cerastes cerastes on nerve tissue and skeletal muscle. | | 1966 | 5938786 |
| rapid screening and identification of ace inhibitors in snake venoms using at-line nanofractionation lc-ms. | this study presents an analytical method for the screening of snake venoms for inhibitors of the angiotensin-converting enzyme (ace) and a strategy for their rapid identification. the method is based on an at-line nanofractionation approach, which combines liquid chromatography (lc), mass spectrometry (ms), and pharmacology in one platform. after initial lc separation of a crude venom, a post-column flow split is introduced enabling parallel ms identification and high-resolution fractionation on ... | 2017 | 28801827 |
| haemostasis disorders caused by envenomation by cerastes cerastes and macrovipera mauritanica vipers. | viper venoms are a real source of proteolytic enzymes causing clotting, bleeding, edema, necrosis, hemorrhage, pain at the bite site and systemic changes. this study was conducted to evaluate the changes induced in hematological and haemostatic parameters in rabbits after 1, 3, 6 and 24 h post-venom of subcutaneously administration of a sublethal dose of cerastes cerastes and macrovipera mauritanica venoms. our results indicated that most hematological and haemostatic parameters showed significa ... | 2016 | 26724273 |
| histology of the skin of three limbless squamates dwelling in mesic and arid environments. | the skin of limbless squamates has an increased contact with the substrate compared with limbed counterparts. comparatively, the contact with the substrate is intensified in fossorial species, where the whole circumference of the body interacts with the soil during underground locomotion. although fossoriality in squamata, specifically lizards and snakes, has been studied ecologically and morphologically (e.g., osteological changes), not enough detail is yet available regarding changes in organs ... | 2016 | 27111253 |
| cross neutralization of dangerous snake venoms from africa and the middle east using the vacsera polyvalent antivenom. egyptian organization for biological products & vaccines. | this study was performed to assess the ability of polyvalent snake venom anti-serum, produced by the egyptian organization for biological products & vaccines (vacsera), to neutralize several toxic activities of snake venoms, not only of those included in the antivenom mixture, but also some additional venoms of snakes from egyptian, african, and middle eastern habitats. in general, the results revealed that polyvalent snake venom anti-serum from vacsera is highly effective in neutralizing egypti ... | 2002 | 12503876 |
| purification of hemorrhagic svmps from venoms of three vipers of egypt. | three viper p-iii hemorrhagic svmps: epyhti (60 kda), ecohti (60 kda) and cchti (58 kda) of the most dangerous vipers echis pyramidum, echis coloratus and cerastes cerastes, respectively were purified and characterized in a set of biochemical assays. the svmps were purified by applying a protocol of three successive chromatographic steps. the enzymatic activity of the purified svmps was stimulated by the divalent cations ca2+, mg2+ and inhibited by metalloproteinase inhibitors and (zn2+, mn2+, n ... | 2012 | 22138485 |
| hemorrhagic metalloproteinase, cc hsm-iii, isolated from cerastes cerastes venom: purification and biochemical characterization. | snake venom metalloproteinases are the most abundant toxins in viperidae venoms. in this study, a new hemorrhagin, cc hsm-iii (66 kda), was purified from cerastes cerastes venom by gel filtration, ion exchange, and reversed-phase high-performance liquid chromatographies. the analysis of cc hsm-iii by liquid chromatography with a tandem mass spectrometry revealed 32 peptides sharing a homology with p-iii metalloproteinases from echis ocellatus snake venom. cc hsm-iii displays hemorrhagic activity ... | 2017 | 28245079 |
| molecular characterisation of endogenous snake venom metalloproteinase inhibitors. | viper venoms contain one of the most potent mixtures of proteases in natural existence and yet the venom gland and proteins in this mixture are refractory to degradation. here we demonstrate that the sub-10-kda components of venom from two african viper species (echis ocellatus and cerastes cerastes cerastes) are predominantly composed of the tri-peptide pyroglutamate-lysine-tryptophan (pekw). this tripeptide is encoded by tandemly repeating elements and, in e. ocellatus, on the same transcript ... | 2008 | 18029259 |
| biochemical and monolayer characterization of tunisian snake venom phospholipases. | the present study investigated the kinetic and interfacial properties of two secreted phospholipases isolated from tunisian vipers'venoms: cerastes cerastes (cc-pla2) and macrovipera lebetina transmediterranea (mvl-pla2). results show that these enzymes have great different abilities to bind and hydrolyse phospholipids. using egg-yolk emulsions as substrate at ph 8, we found that mvl-pla2 has a specific activity of 1473u/mg at 37°c in presence of 1mm cacl2. furthermore the interfacial kinetic an ... | 2016 | 27164498 |
| pathophysiological effects of cerastes cerastes and vipera lebetina venoms: immunoneutralization using anti-native and anti-(60)co irradiated venoms. | cerastes cerastes and vipera lebetina are the most medically important vipers in algeria. their bite induces several pathological effects on victims of accidental envenomation. in this study we analyzed the pathogenesis induced after an experimental envenomation. indeed, we determined, in vitro, venom enzymatic activities and we analyzed, in vivo, pathological effects induced on liver, heart, lung and skin. in addition we investigated the neutralizing potency of four experimental antivenoms elic ... | 2016 | 26678662 |
| antitumoral potential of tunisian snake venoms secreted phospholipases a2. | phospholipases type a2 (pla2s) are the most abundant proteins found in viperidae snake venom. they are quite fascinating from both a biological and structural point of view. despite similarity in their structures and common catalytic properties, they exhibit a wide spectrum of pharmacological activities. besides being hydrolases, secreted phospholipases a2 (spla2) are an important group of toxins, whose action at the molecular level is still a matter of debate. these proteins can display toxic e ... | 2013 | 23509718 |
| snake venomics: comparative analysis of the venom proteomes of the tunisian snakes cerastes cerastes, cerastes vipera and macrovipera lebetina. | the protein composition of the crude venoms of the three most important vipers of tunisia was analyzed by rp-hplc, n-terminal sequence analysis, maldi-tof mass determination, and in-gel tryptic digestion followed by pmf and cid-ms/ms of selected peptide ions in a quadrupole-linear it instrument. our results show that the venom proteomes of cerastes cerastes, cerastes vipera, and macrovipera lebetina are composed of proteins belonging to a few protein families. however, each venom showed distinct ... | 2005 | 16206329 |
| grape seed extract neutralizes the effects of cerastes cerastes cerastes post-synaptic neurotoxin in mouse diaphragm. | this work was undertaken to investigate the toxic activity of the post-synaptic neurotoxic fraction isolated from the venom of the egyptian sand viper (cerastes cerastes cerastes), and the ability of grape seed extract to antagonize this effect produced in sublethally intoxicated mice, with an emphasis on ultrastructural features. light and transmission electron microscopy of diaphragms of intoxicated mice showed myonecrosis, myofiber hypercontraction, sarcomere disorganization, and mitochondria ... | 2013 | 22917896 |
| intercontinental test of constraint-breaking adaptations: testing behavioural plasticity in the face of a predator with novel hunting strategies. | constraint-breaking adaptations are evolutionary tools that provide a mechanism for incumbent-replacement between species filling similar ecological roles. in common-garden experiments, we exposed populations of two desert rodents to two different viper species, testing their ability to adjust to novel predators that use different hunting strategies. we aimed to understand whether both predators and prey with constraint-breaking adaptations actually manifest comparative advantage over their coun ... | 2020 | 32271948 |
| comparative study of the in vivo toxicity and pathophysiology of envenomation by three medically important egyptian snake venoms. | snakebite envenomation is a serious medical problem in many developing tropical and subtropical countries. envenomation is registered by the world health organization as a neglected tropical disease due to critical shortages in the production of antivenom. envenomation causes more than 100,000 deaths annually. snakebites result in several effects to include edema, blistering, hemorrhage, necrosis and respiratory paralysis. antivenom is the preferred treatment for the systemic effects of snakebit ... | 2020 | 31722041 |
| cerastes cerastes snakebite complicated by coagulopathy and cardiotoxicity with electrocardiographic changes. | snakebites occur worldwide with varying frequency. outcomes associated with snakebite are varied and depend on the type of snake and the general health of the patient. we report a case of snakebite by cerastes cerastes or desert horned viper. consumptive coagulopathy and cardiotoxicity with electrocardiographic changes complicated the clinical course of the patient. to the best of our knowledge, this is the first reported case in the state of qatar. | 2020 | 33035563 |
| embryonic development of skull bones in the sahara horned viper (cerastes cerastes), with new insights into structures related to the basicranium and braincase roof. | ontogenetic studies are crucial for understanding functional morphology, origin and adaptation of skulls in vertebrates. however, very few studies have so far released complete embryonic series focusing on skull embryonic development in species showing diverse and extreme cranial morphologies such as snakes. the wide distribution and unique reproductive and ecological behaviors of venomous vipers, including the heterogeneity in breeding and egg incubation periods in oviparous species, make this ... | 2020 | 32242931 |
| a horned viper bite victim with pres. | neurological complications of snake bites have been well documented in the literature as neuromuscular paralysis and cerebrovascular complications; posterior reversible encephalopathy syndrome was rarely described. a 23-year-old lady presented near full term of her pregnancy with a horned snake cerastes cerastes bite; after successful delivery she started complaining of altered mental status and visual disturbance with ulceration over the site of the snake bite. on admission, the patient had gla ... | 2017 | 28487791 |
| ecological aspects of the horned viper, cerastes cerastes gasperettii in the central region of saudi arabia. | feeding ecology of any species can help us to understand its natural history, ecological requirements and approaches involved in searching for food. feeding ecology and sexual dimorphism in the horned viper, cerastes cerastes gasperetti collected from the al-thumama area, central region of saudi arabia was described. the gut content of cerastes c. gasperetti mainly consisted of rodents (70%) in addition to arthropods (15%) and lizards (10%). least sexual size dimorphism was noticed in the specie ... | 2016 | 26858550 |
| reproductive biology of the horned viper, cerastes cerastes gasperettii in the central region of saudi arabia. | the reproductive biology of the horned viper, cerastes cerastes gasperettii, in riyadh region of saudi arabia was investigated over a period of one year. study of reproductive cycle of male and female c. c. gasperettii revealed that the breeding season is relatively short (april and may). thereafter females laid eggs by mid of july and hatching probably had taken place by the end of september. no activity was observed during winter, this may indicate just a single clutch per year. relative testi ... | 2015 | 25972758 |
| [association of ischemic and hemorrhagic cerebral stroke due to severe envenomation by the sahara horned viper (cerastes cerastes)]. | viper envenomation can cause complex disorders of hemostasis. these disorders usually lead to hemorrhagic syndrome. the occurrence of thrombosis is exceptional. this case report describes a severe envenomation by the saharan horned viper (cerastes cerastes), complicated by unusual brain injuries: hemorrhagic and ischemic stroke. the physiopathologic link between this serious toxic coagulopathy and the multitude of toxins in the venom of cerastes cerastes is highlighted. | 2013 | 23934315 |