| comparison of antibodies in marine fish from clean and polluted waters of the new york bight: relative levels against 36 bacteria. | fish from polluted waters are subject to increased prevalence of disease. because they respond to bacterial pathogens by producing serum antibodies, it was possible to construct a seasonal serological record in three fish species from clean and polluted waters of the new york bight. antibody levels were determined by testing sera for agglutinating activity against 36 strains of bacteria. evaluation of 5,100 antibody titrations showed the following. during warm months, summer flounder (paralichth ... | 1979 | 518084 |
| determinants of the renal handling of 2, 4-dichlorophenoxyacetic acid by winter flounder. | the factors determining the renal handling of 2,4-dichlorophenoxyacetic acid (2,4-d) were examined in winter flounder, pseudopleuronectes americanus, using isolated tubules and clearance techniques. in vitro, extensive energy-dependent uptake was seen with tissue/medium ratios of 30-fold at 1 micronm 2,4-d. the velocity of uptake was concentration-dependent with apparent km and vmax v,lues of 70 micronm and 3.6 micronmol/g of tubules per hr, respectively. uptake was inhibited by other organic ac ... | 1979 | 762661 |
| the phlorizin effect on the transport of sugars at the antiluminal face of teased flounder tubules. | the effect of phlorizin on the uptake of sugars by teased renal tubules of the flounder (pseudopleuronectes americanus) and slices of rabbit kidney cortex was investigated: 1. increasing concentrations (0.05-0.5 mm) of phlorizin inhibited the uptake of d-glucose at the antiluminal face of the tubular cells by affecting primarily the cellular levels of glucose phosphates, whereas the levels of free glucose remained constant. this result suggests the possibility that the actual transport step is a ... | 1977 | 850118 |
| ultrastructure of the peripheral zone of a glugea-induced xenoma. | the glugea stephani-induced xenoma in the winter flounder, pseudopleuronectes americanus, is a large spherical host-parasite complex, up to 4.0 mm in diameter, with the host and parasite components of the xenoma being most active in the peripheral zone. the xenoma has an extensive periodic acid-silver methenamine-positive surface coat covering the plasma membrane. the surface of this membrane is amplified by the presence of numerous folds and fine tubular extensions. the peripheral zone of the x ... | 1976 | 933079 |
| the synthesis of freezing-point-depressing protein of the winter flounder pseudopleuronectus americanus in xenopus laevis oocytes. | | 1976 | 986821 |
| inhibition of ca2+ and k+ currents by "antifreeze" proteins. | for the last two decades, the research on fish "antifreeze" proteins has focused exclusively on their ability to depress noncolligatively blood plasma freezing points, presumably by binding to ice crystals. we report evidence that antifreeze polypeptides from the winter flounder (pseudopleuronectes americanus) have another special property, the ability to block ion channels. in experiments with porcine granulosa cells we show, using the patch-clamp technique in the whole cell configuration, that ... | 1992 | 1313654 |
| sarcoplasmic reticulum from rabbit and winter flounder: temperature-dependence of protein conformation and lipid motion. | a comparative study of lipids and proteins in sarcoplasmic reticulum (sr) from rabbit and flounder has been undertaken. the protein/phospholipid ratio (w/w) was 3:1 in flounder sr (fsr) and 2.2:1 in rabbit sr (rsr). both membranes had similar contents of pc (70%) and pi (6%). pe constituted 15% in rsr and 21% in fsr. ps and sphingomyelin were minor components of both sr (less than 4%). there were differences in the unsaturated chains of the total lipid extracts, pc, pe, and pi between fsr and rs ... | 1992 | 1535511 |
| thermoprotection of a functional epithelium: heat stress effects on transepithelial transport by flounder renal tubule in primary monolayer culture. | primary monolayer cultures of winter flounder renal proximal-tubule cells were used to determine whether transepithelial transport could be protected from the damaging effects of extreme temperature by previous mild heat shock. renal tubule epithelial cells were enzymatically dispersed and reorganized as confluent monolayer sheets on native rat tail collagen. transepithelial electrical properties (potential difference, resistance, short-circuit current, and na(+)-dependent glucose current) and u ... | 1992 | 1565616 |
| enhanced survival of yeast expressing an antifreeze gene analogue after freezing. | yeast, like most organisms, survives poorly under freezing conditions. it has been proposed that after rapid cooling yeast suffers a loss in viability from the recrystallization of intracellular ice. antifreeze proteins found in the blood of certain polar fishes have been shown to be potent inhibitors of ice recrystallization at very low concentrations. we have examined the feasibility of protecting rapidly cooled yeast cells from freezing damage by inhibiting the recrystallization of intracellu ... | 1991 | 1752135 |
| taurine secretion in cultured winter flounder renal epithelium. | the mechanism for the secretion of taurine by the marine fish kidney was examined in flounder renal primary cultures and teased tubules. the question addressed was whether the secretion of taurine, but not beta-alanine, a related beta-amino acid, is due to differences in their basolateral uptake, intracellular concentration, or luminal efflux mechanism. aminooxyacetate (aoa) (10 mm), an inhibitor of beta-alanine oxidation, allowed the basolateral beta-alanine accumulation (measured in teased tub ... | 1991 | 1951764 |
| hypothermic protection--a fundamental property of "antifreeze" proteins. | for the last two decades fish antifreeze proteins have been considered to function exclusively in conferring freeze-resistance to fish by binding to ice crystals and thereby depressing blood plasma freezing points non-colligatively. we report here the discovery of a second fundamental property of antifreeze proteins, the ability to protect cells and their membranes from hypothermic damage. experiments were carried out exposing immature bovine oocytes to 4 degrees c for 24 h in the presence of ty ... | 1991 | 1953726 |
| inhibition of recrystallization in ice by chimeric proteins containing antifreeze domains. | using synthetic dna, we assembled a gene encoding a protein identical in sequence to one of the antifreeze proteins produced by the fish pseudopleuronectes americanus (winter flounder). to address the relationship between structure and function, we also assembled genes encoding proteins varying in sequence and length. the synthetic genes were cloned into a bacterial expression vector to generate translational fusions to the 3' end of a truncated staphylococcal protein a gene; the chimeric protei ... | 1991 | 2019569 |
| carcinogenesis studies in rodents for evaluating risks associated with chemical carcinogens in aquatic food animals. | fish and shellfish caught in polluted waters contain potentially dangerous amounts of toxic and carcinogenic chemicals. public concern was heightened when a large percentage of winter flounder taken from boston harbor was found to have visible cancer of the liver; winter flounder outside the estuary area had no liver lesions. long-term chemical carcinogenesis studies could be easily and feasibly designed using laboratory rodents offered diets containing fish caught in polluted waters. induced ca ... | 1991 | 2050050 |
| temperature dependence of kinetic parameters of (ca2+ + mg2+)-atpase in rabbit and winter flounder sarcoplasmic reticulum. | the effect of temperature on the activity of (ca2+ + mg2+)-atpase in rabbit (r) and winter flounder (f) sarcoplasmic reticulum (sr) has been investigated. the enzymes from the two sources appear to be differently adapted to temperature. (ca2+ + mg2+)-atpase activity of fsr was high at 35 degrees c, and declined to zero at 40 degrees c, whereas rsr was still active above 45 degrees c. km for atp increased with temperature in a biphasic fashion for both enzymes. the km values for fsr were 69-75 mi ... | 1990 | 2148272 |
| second messenger-specific protein kinases in a salt-absorbing intestinal epithelium. | calcium, adenosine 3',5'-cyclic monophosphate (camp), and guanosine 3',5'-cyclic monophosphate (cgmp) can regulate the same or different ion transport processes within an epithelium, presumably via independent protein phosphorylation mechanisms. because there have been few detailed studies characterizing these processes in epithelia, we examined the distribution of ca-, camp-, and cgmp-specific protein kinases and substrates in vitro in a homogenous salt-absorbing epithelium, the winter flounder ... | 1990 | 2159231 |
| design and cloning of a synthetic gene for the flounder antifreeze protein and its expression in plant cells. | a synthetic gene coding for the winter flounder antifreeze protein (afp) has been constructed. a new strategy for the synthesis has been employed such that one strand of the duplex was chemically synthesized and the other was produced enzymatically by chain extension. the chemically synthesized blocks were constructed so that the second strand was self-priming. the resulting dna fragment was incorporated into the vector, pgcs1, which contained a translational fusion of the sequence encoding afp ... | 1990 | 2210378 |
| fasting induces modifications of the endoplasmic reticulum in intestinal cells. | the polymorphism of the endoplasmic reticulum (er) in epithelial cells with different transport functions such as the enterocyte suggests that the er may be involved in some way in molecular transport. to further access this possibility, we examined the er from the intestine of winter flounder, pseudopleuronectes americanus, a species which undergoes an annual fast of approximately 6 months' duration, a time during which previous work indicates nutrient carrier number does not change. fish from ... | 1990 | 2213232 |
| evidence for chloride secretion in the intestine of the winter flounder. | the effects of a novel antisecretory peptide (cap) isolated from porcine heart and vasoactive intestinal peptide (vip) on ion transport were investigated in the winter flounder intestine. partially purified cap caused a two- to sixfold increase in the serosa-negative short-circuit current (isc) with no significant change in tissue conductance. cap significantly inhibited the serosal-to-mucosal (s-m) unidirectional cl flux without affecting either na or rb transepithelial fluxes. the isc after th ... | 1990 | 2305867 |
| mutations in c-ki-ras oncogenes in diseased livers of winter flounder from boston harbor. | livers of a natural population of winter flounder from a contaminated site in boston harbor were examined for the presence of oncogenes by transfection of dna into nih 3t3 mouse fibroblasts. tissues analyzed contained histopathologic lesions including abnormal vacuolation, biliary proliferation, and, in many cases, hepatocellular and cholangiocellular carcinomas. fibroblasts transfected with liver dna samples from 7 of 13 diseased animals were effective in the induction of subcutaneous sarcomas ... | 1990 | 2405390 |
| the effect of the microsporida glugea stephani on the immunoglobulin levels of juvenile and adult winter flounder (pseudopleuronectes americanus). | the microsporida glugea stephani is an intracellular parasite that causes a decline in the serum igm levels of both adult and juvenile winter flounder, shortly after introduction. this decline was noted in fish injected with spores or infected via an intermediary vector. the parasite also suppresses the humoral response to other antigens such as horse red blood cells. the suppression of the response to the antigen however, is related directly to the exposure time with regard to the kinetic state ... | 1989 | 2504626 |
| biosynthesis of winter flounder antifreeze proprotein in e.coli. | a semisynthetic winter flounder antifreeze proprotein (proafp) coding region was constructed and inserted into a lacz expression vector. proafp was produced from the vector in escherichia coli as a c-terminal fusion to the first 289 amino acids of beta-galactosidase (beta-gal). the proafp and beta-gal domains of the beta-gal-proafp fusion protein were separated by the recognition signal for the blood coagulation protease, factor xa. upon induction with isopropylthio-beta-d-galactoside the fusion ... | 1989 | 2687848 |
| wolffish antifreeze protein genes are primarily organized as tandem repeats that each contain two genes in inverted orientation. | the antifreeze protein genes of the wolffish (anarhichas lupus) constitute a large multigene family of 80 to 85 copies, which can be classified into two sets. one-third of the genes were linked but irregularly spaced. the other two-thirds were organized as 8-kilobase-pair (kbp) tandem direct repeats that each contained two genes in inverted orientation; dna sequence analysis suggests that both genes are functional. except for a single region specific to each gene, the genes and their immediate f ... | 1988 | 2851724 |
| solute effects on mitochondria from an elasmobranch (raja erinacea) and a teleost (pseudopleuronectes americanus). | varying osmolarity with sucrose/kcl media resulted in similar effects on the oxidation of glutamate by mitochondria isolated from the livers of an elasmobranch, raja erinacea, and a teleost, pseudopleuronectes americanus. in both species trimethylamine oxide (tmao) inhibited mitochondrial oxidation of glutamate. urea penetrated the inner mitochondrial membrane of both species and equilibrated with a ratio ureai/ureao of unity. urea had little effect on the oxidation of glutamate in both species ... | 1986 | 2876050 |
| the immune response of a marine teleost, pseudopleuronectes americanus, (winter flounder) to the protozoan parasite glugea stephani. | g. stephani is an intracellular cyst-forming microsporidan parasite that is found in the intestine of winter flounder (wf) pseudopleuronectes americanus. no detectable humoral response was seen in parasitized fish or in fish injected with either spores or spore homogenate from this parasite. quantification of total immunoglobulin (ig) levels showed a decrease in ig levels rather than enhancement, 21 days after intramuscular (im) injections of spores (3 x 10(6)/ml). when a second injection of spo ... | 1986 | 3094232 |
| immunoglobulin levels of the winter flounder (pseudopleuronectes americanus) and the summer flounder (paralichthys dentatus) injected with the microsporidan parasite glugea stephani. | the microsporidan parasite g. stephani, has been found in the intestine of the american winter flounder pseudopleuronectes americanus. a quantitative study of total serum immunoglobulin (ig) levels has shown that an injection of this parasitic spore causes a decrease in serum igm levels. the level of suppression seems to be related to the number of spores injected into the host, as well as the frequency of injections administered. as the dosage of spores injected into the winter flounder increas ... | 1986 | 3095157 |
| birefringent spores differentiate encephalitozoon and other microsporidia from coccidia. | tissue sections containing protozoa with birefringent spores indicate an infection by microsporidia. hematoxylin and eosin (he) does not affect spore birefringence, but some special stains (goodpasture, brown and brenn, or gram) obscure it. encephalitozoon cuniculi from an infected puppy, glugea stephani from the winter flounder pseudopleuronectes americanus, and plistophora sp. from the japanese eel anguilla japonica all have birefringent spores. encephalitozoon was studied first and then the t ... | 1988 | 3134761 |
| cutaneous tumor of northern pike, esox lucius l. ii. expression of surface and cytoplasmic immunoglobulins and alpha-naphthyl acetate esterase by mononuclear cells from lymphoid tissues and tumor. | rabbit anti-pike igm antibodies showed that up to 95% of mononuclear (mn) cells isolated on ficoll-isopaque gradients from peripheral blood, spleen, and head kidney of northern pikes carrying cutaneous tumors were surface (sig) and cytoplasmic (cig) immunoglobulin positive by indirect immunofluorescence. on the contrary, rabbit anti-winter flounder igm antibodies gave totally negative results. monoclonal anti-carp igm antibodies stained approximately 40-56% of peripheral blood, splenic, and head ... | 1988 | 3350193 |
| specificity and cross-reactivity of monoclonal and polyclonal antibodies against cytochrome p-450e of the marine fish scup. | monoclonal antibody (mab) 1-12-3 generated against liver cytochrome p-450e (p-450e), an aryl hydrocarbon hydroxylase of the marine fish stenotomus chrysops (scup), reacted only with p-450e when tested in immunoblot analysis with five p-450 fractions from scup liver. this and six other mabs against p-450e recognized purified p-450e, as well as a single band in beta-naphthoflavone (bnf)-induced scup microsomes that comigrated with authentic p-450e. like mab 1-12-3, polyclonal anti-p-450e reacted w ... | 1987 | 3545085 |
| na-k-2cl cotransport in winter flounder intestine and bovine kidney outer medulla: [3h] bumetanide binding and effects of furosemide analogues. | the effects of several sulfamoyl benzoic acid derivatives on na-k-cl cotransport were investigated in winter flounder intestine. the relative efficacy (ic50 values) and order of potency of these derivatives were benzmetanide, 5 x 10(-8) m greater than bumetanide 3 x 10(-7) m greater than piretanide 3 x 10(-6) m greater than furosemide 7 x 10(-6) m greater than amino piretanide 1 x 10(-5) 3-amino-4-penoxy-5-sulfamoyl benzoic acid. binding of [3h] bumetanide was studied in microsomal membranes fro ... | 1987 | 3585983 |
| vibrio anguillarum as a cause of disease in winter flounder (pseudopleuronectes americanus). | | 1972 | 5081124 |
| antifreeze protein genes of the winter flounder. | a genomic library of the winter flounder in lambda phage charon 4 was screened with antifreeze protein cdna. nine genomic clones were isolated from three haploid genome equivalents of phage and were grouped into three classes on the basis of restriction mapping and heteroduplex analysis. the three chromosomal regions are non-overlapping and each contain two antifreeze protein genes spaced from 3 to 7 kilobase pairs apart. genomic southern blots indicate that these six non-allelic genes represent ... | 1984 | 6086629 |
| molecular cloning and characterization of winter flounder antifreeze cdna. | double-stranded cdna was synthesized from partially purified winter flounder antifreeze mrna and inserted into the endonuclease pst i site of plasmid pbr322 by the poly(dg).poly(dc) homopolymer extension technique. the recombinant plasmids wee used to transform escherichia coli. clones containing antifreeze cdna inserts were identified by the hybridization-selection technique. one of the inserts, 380 nucleotides in length, was digested with endonucleases sau3ai and hinfi, which cleaved the inser ... | 1981 | 6265915 |
| effects of crude oils on the gastrointestinal parasites of two species of marine fish. | to assess the effect of crude oil on selected gastrointestinal parasites of fish, winter flounder (pseudopleuronectes americanus) naturally infected with a digenetic trematode (steringophorus furciger), were exposed to contaminated sediment or water soluble fractions of venezuelan crude oil for 34 and 160 days respectively. similarly, atlantic cod (gadus morhua) harboring an acanthocephalan (echinorhynchus gadi), were treated with extracts of hibernia and venezuelan oils for periods of 81 to 140 ... | 1983 | 6644923 |
| flounder intestinal absorptive cells have abundant gap junctions and may be coupled. | we noted that, unlike mammalian intestinal absorptive cells, cells of the winter flounder (pseudopleuronectes americanus) displayed abundant gap junctions on the lateral plasma membrane. we compared the distribution of gap junctions in winter flounder to that in rabbit intestinal epithelium. we also examined for evidence of gap junction-mediated intercellular coupling by comparing the cell-to-cell variation of electrical potential difference across winter flounder intestinal cell apical membrane ... | 1984 | 6696060 |
| a comparison of temperature-mediated immunomodulation between two species of flounder. | the kinetics of the temperature-mediated immunomodulation of the humoral antibody response to an erythrocyte antigen was remarkably different in two species of a marine teleost, the winter flounder (pseudopleuronectes americanus) and the summer flounder (paralichthys dentatus). these two species were caught in the same area, maintained under the same conditions and injected with the same antigen (horse red blood cells). experiments were performed at 8, 12 and 17 degrees c during the same time of ... | 1984 | 6745989 |
| seasonal variation in the level of antifreeze protein mrna from the winter flounder. | livers from winter flounder collected at monthly intervals throughout the year were analyzed for their content of antifreeze protein mrna. putative mrna was detected in liver rnas from summer fish by liquid hybridization to kinetically purified antifreeze protein cdna. these mrna sequences were shown by northern blot analysis to be of the same length as mature antifreeze protein mrna isolated from winter fish, and were able to direct the incorporation of alanine into a translation product which ... | 1983 | 6830805 |
| effects of teleost gonadotropins and their antibodies on gonadal histology in winter flounder. | | 1980 | 7002714 |
| renal handling of taurine in marine fish. | net renal tubular excretion of taurine was demonstrated by clearance techniques in the dogfish, squalus acanthias; the little skate, raja erinacea; and in the winter flounder, pseudopleuronectes americanus. dogfish acclimated to 70% seawater had increased renal excretion of taurine resulting from higher glomerular filtration rates of taurine as well as from increased rats of tubular secretion. tubular secretion of taurine prevailed in the dogfish and flounders measured, whereas it occurred in on ... | 1982 | 7058931 |
| renal sugar transport in the winter flounder. vi. reabsorption of d-mannose. | the transport of d-mannose (man) in flounder kidney was studied using renal clearance techniques in vivo and brush border membrane (bbm) vesicles in vitro. at plasma concentrations of 50-100 microm man, the winter flounder (pseudopleuronectes americanus) reabsorbed up to 70% of the filtered sugar. man phosphates, but not free man, accumulated in renal cells. reabsorption of man was reduced by phlorizin, d-glucose, methyl-alpha-d-glucoside, methyl-alpha-d-mannoside, and 2-deoxy-d-glucose. in bbm ... | 1982 | 7065250 |
| radioimmunoassay for the antifreeze polypeptides of the winter flounder: seasonal profile and immunological cross-reactivity with other fish antifreezes. | the polymerization of winter flounder antifreeze polypeptide (afp) with glutaraldehyde to increase its antigenicity and the conjugation of the afp to bovine serum albumin so that the afp could carry a radioiodine label have allowed the development of a convenient and reliable radioimmunoassay (ria) for the antifreeze. a seasonal concentration profile of serum afp as determined by the ria corresponded closely with its activity profile. winter serum concentrations of afp measured by the ria (7-11 ... | 1982 | 7127188 |
| acid-soluble nuclear proteins of the testis during spermatogenesis in the winter flounder. loss of the high mobility group proteins. | | 1980 | 7358686 |
| isolation and characterization of the antifreeze protein messenger rna from the winter flounder. | the messenger rna coding for the serum antifreeze protein of the winter flounder (pseudopleuronectes americanus) was extracted from the liver polysomes of fish caught in november and was purified by oligo(dt)-cellulose chromatography and sucrose density gradient centrifugation. the length of the mrna was 520 nucleotides measured by agarose gel electrophoresis in the pesence of methyl mercury. it sedimented between trout protamine mrna (6 s) and rabbit globin globin mrna (9-10 s) on sucrose densi ... | 1980 | 7410390 |
| [cloning and expression of an antifreeze peptide gene of winter flounder in e. coli]. | antifreeze peptide (afp) produced in some animals is able to lower freezing temperature to prevent serum from freezing. there is a great potential to apply afp in various circumstances wherever tolerance to cold environment is required. cdna copies of afp and proafp coding sequence were synthesized according to the published sequence from a winter flounder (pseudopleuronectes americanus). expression of afp or proafp cdna was initially tested in e. coli using a procaryotic expression vector. leve ... | 1995 | 7597872 |
| the effects of supercooling chemicals on myocardial ultrastructure: a transmission electron microscopy case study. | extended ischemia results in organ infarction which limits the availability of donor hearts. hypothermic storage extends heart preservation by effectively stopping cellular metabolism, thereby preventing toxic accumulations of metabolic wastes and depletion of energy stores. however, cell swelling as a result of ion concentration changes and cell laceration due to ice crystal growth are consequences of hypothermic ischemia. supercooling successfully preserves hearts for an extended time without ... | 1995 | 7671597 |
| production of a biologically active recombinant teleostean growth hormone in e. coli cells. | we have isolated and characterized several recombinant lambda phage clones carrying growth hormone (gh) cdna of striped bass (morone saxatilis). nucleotide sequence and the predicted amino acid sequence of sbgh was determined from a recombinant clone carrying the longest cdna insert. the sbgh cdna encodes a pre-hormone of 204 amino acid residues. comparison of the predicted amino acid sequence of sbgh with those of other vertebrates revealed different degrees of sequence identity: approximately ... | 1995 | 7758842 |
| effect of somatolactin and related hormones on phosphate transport by flounder renal tubule primary cultures. | winter flounder renal proximal tubule primary monolayer cultures mounted in ussing chambers were used to determine the effect of salmon somatolactin (ssl) on transepithelial pi and ca2+ transport. ssl stimulated pi reabsorption in a dose-dependent manner at physiological levels of the hormone (12.5 ng/ml). net pi transport was significantly altered by ssl (200 ng/ml) within 2 h after the initial exposure. ca2+ fluxes were unchanged by the addition of 200 ng/ml ssl. the ssl-induced pi reabsorptio ... | 1995 | 7900898 |
| stanniocalcin stimulates phosphate reabsorption by flounder renal proximal tubule in primary culture. | the effects of several hormones on transepithelial pi transport were determined in primary monolayer cultures of winter flounder proximal tubule epithelium in ussing chambers. salmon stanniocalcin (stc) had a dose-dependent stimulatory effect on net pi reabsorption within the normal plasma hormone concentration range, 12.5-50 ng/ml (0.25-1.0 nm). net pi transport was significantly altered by stc (200 ng/ml) within 30 min and progressively increased from slight net secretion (0.26 +/- 0.744 nmol. ... | 1994 | 7977865 |
| effect of antifreeze proteins on the motility of ram spermatozoa. | the effect of the antifreeze peptide type i (afp) from winter flounder, pseudopleuronectes americanus, and the antifreeze glycoprotein (afgp) from antarctic cod, dissostichus mawsoni, was assessed on the motility of ram spermatozoa (dorset and dorset x romney) after chilling (5 degrees c) and after freeze-thawing. during chilling, spermatozoal motility decreased significantly at an afp or afgp concentration of 0.1 microgram/ml and at concentrations above 10 micrograms/ml (p < 0.05). thus, at 5 d ... | 1994 | 8004998 |
| cloning and expression of a renal na-pi cotransport system from flounder. | starting with the recently published sequence of the rat renal na-pi cotransport system, we have cloned a corresponding cdna from the kidney of winter flounder (pseudopleuronectes americanus), designated flounder napi-ii. expression of the cognate in vitro transcribed rna in xenopus laevis oocytes stimulated na-dependent pi transport specifically and in a time- and dose-dependent manner. apparent affinities of na and pi, as well as the ph dependency, were very similar to those found for the mamm ... | 1994 | 8067391 |
| photoaffinity labeling of the ah receptor: phylogenetic survey of diverse vertebrate and invertebrate species. | the mammalian aromatic hydrocarbon (ah) receptor is a soluble protein involved in the regulation of gene expression by halogenated aromatic hydrocarbons such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd). little is known, however, about the presence and properties of this receptor in nonmammalian species. in these studies, we sought evidence for an ah receptor in the liver or liver-equivalent of diverse species of invertebrate and vertebrate animals. velocity sedimentation analysis of hepatic cy ... | 1994 | 8161208 |
| accumulation of type i fish antifreeze protein in transgenic tobacco is cold-specific. | expression of fish antifreeze protein (afp) genes in plants is a possible means of increasing their frost resistance and freeze tolerance. initial work involved transfer into tobacco of an afp gene from winter flounder which codes for the alanine-rich, alpha-helical type i afp. plants were transformed with a gene construct in which the preproafp cdna was inserted between the cauliflower mosaic virus 19s rna promoter and the nopaline synthetase polyadenylation site. although transgenic plants pro ... | 1993 | 8219071 |
| expression and structural analysis of a teleost homolog of a mammalian zona pellucida gene. | gene expression in oviparous vertebrates during vitellogenesis is hormonally regulated. our laboratory has characterized a unique gene (wf female), which is seasonally expressed in the liver of the female winter flounder pseudopleuronectes americanus. the wf female mrna is coexpressed with vitellogenin mrna and reaches a high level during vitellogenesis. the wf female gene is 2554 base pairs in length and encodes a putative protein of 509 amino acids. the gene consists of eight exons separated b ... | 1993 | 8407974 |
| na-pi cotransport in flounder: same transport system in kidney and intestine. | the cloning of a renal na-pi contransport in system from winter flounder (p eudopleuronectes americanus) has recently been reported. we used this information to answer the questions 1) what is the distribution of the transport protein along the nephron? and 2) how are renal and intestinal transporters related? the distribution of the flounder napi-ii protein was tested using two antisera raised against partial sequences (amino acids 1-14 and 388-441) of the transporter. antibody-specific fluores ... | 1996 | 8764312 |
| directed evolution of a type i antifreeze protein expressed in escherichia coli with sodium chloride as selective pressure and its effect on antifreeze tolerance. | both freezing tolerance and nacl tolerance are improved when antifreeze proteins are expressed as fusion proteins with two domains of staphylococcal protein a (spa) in escherichia coli. to characterize these properties further we created a randomly mutated expression library in e. coli, based on the winter flounder antifreeze protein hplc-8 component gene. low-fidelity pcr products of this gene were fused to the spa gene encoding two domains of the spa. the library was screened for enhanced nacl ... | 1996 | 8961358 |
| isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder. | antimicrobial peptides are found in both myeloid cells and mucosal tissues of many vertebrates and invertebrates. these peptides are predicted to operate as a first-line host defense mechanism exerting broad-spectrum activity against pathogenic bacteria, fungi, parasites, and enveloped viruses. we report the characterization of a novel 25-residue linear antimicrobial peptide found in the skin mucous secretions of the winter flounder (pleuronectes americanus). this peptide was purified through mu ... | 1997 | 9115266 |
| characterization of a heat-stable fraction of lipovitellin and development of an immunoassay for vitellogenin and yolk protein in winter flounder (pleuronectes americanus). | an enzyme-linked immunoabsorbent assay was developed for detection and quantification of the yolk protein lipovitellin (lv) and its plasma precursor, vitellogenin (vg), in winter flounder (pleuronectes americanus). native lv was found to be a mixture of heat-stable and heat-labile molecules in mature, ovulated eggs. a heat-stable lv fraction was purified from extracts of unfertilized eggs by brief heat treatment and gel permeation chromatography on bio-gel a-1.5. sodium dodecyl sulfate polyacryl ... | 1997 | 9181695 |
| the role of ccaat/enhancer-binding protein alpha and a protein that binds to the activator-protein-1 site in the regulation of liver-specific expression of the winter flounder antifreeze protein gene. | winter flounder, pleuronectes americanus, produces antifreeze proteins (afps) to avoid freezing during winter. the afp in the blood is synthesized by the liver and its annual level is regulated by environmental factors and somatotropin. the only intron (+106 to +602) of the afp gene contains putative binding sites for liver-enriched transcription activators. due to the lack of an appropriate fish liver cell line, a mammalian model system was used to demonstrate the liver-specific enhancer activi ... | 1997 | 9249007 |
| electrophysiological characterization of the flounder type ii na+/pi cotransporter (napi-5) expressed in xenopus laevis oocytes. | the two electrode voltage clamp technique was used to investigate the steady-state and presteady-state kinetic properties of the type ii na+/pi cotransporter napi-5, cloned from the kidney of winter flounder (pseudopleuronectes americanus) and expressed in xenopus laevis oocytes. steady-state pi-induced currents had a voltage-independent apparent k(m) for pi of 0.03 mm and a hill coefficient of 1.0 at neutral ph, when superfusing with 96 mm na+. the apparent k(m) for na+ at 1 mm pi was strongly ... | 1997 | 9351888 |
| expression cloning and characterization of a renal organic anion transporter from winter flounder. | the cdna coding for a renal p-aminohippurate (pah) transporter from winter flounder (pseudopleuronectes americanus), designated froat, was cloned by functional expression in xenopus laevis oocytes. froat is approximately 2.8 kbp in length and encodes a protein of 562 amino acids, related to the rat renal organic anion transporter roat1/oat1 and the organic cation transporters oct1 and oct2. in oocytes, froat mediated probenecid-sensitive pah uptake, with a km for pah of about 20 microm, and inhi ... | 1997 | 9409735 |
| the na+-phosphate cotransport system (napi-ii) with a cleaved protein backbone: implications on function and membrane insertion. | 1. renal handling of inorganic phosphate (pi) involves a na+-pi cotransport system which is well conserved between vertebrates. the members of this protein family, denoted napi-ii, share a topology with, it is thought, eight transmembrane domains. the transporter is proposed to be proteolytically cleaved within a large hydrophilic loop in vivo. 2. the consequences of an interrupted backbone were tested by constructing cdna clones encoding different n- (1-3 and 1-5) and c-terminal (4-8 and 6-8) c ... | 1998 | 9508800 |
| identification of nuclear proteins interacting with the liver-specific enhancer b element of the antifreeze protein gene in winter flounder. | the intron of the winter flounder antifreeze protein (afp) gene contains a liver-specific enhancer element b as demonstrated by transient expression in mammalian cells. element b interacts with rat c/ebpalpha and a novel protein, tentatively designated as the antifreeze enhancer-binding protein (aep). present studies revealed that nuclear proteins from the winter flounder liver interact similarly and specifically to element b as shown by footprinting analysis and gel retardation assays. the pres ... | 1998 | 9701614 |
| chlorzoxazone metabolism by winter flounder liver microsomes: evidence for existence of a cyp2e1-like isoform in teleosts. | winter flounder (pleuronectes americanus) liver microsomes metabolized chlorzoxazone (czx), a cyp2e1 (cytochrome p450 2e1)- specific substrate, to a single product, 6-oh czx. the liver microsomal fraction had the highest level of activity, which varied among fish, ranging from 60 to 220 pmol/mg/min. czx metabolism was linearly related to microsomal protein and dependent on nadph. the optimal ph range for this activity was from 7.2 to 8.0. maximal activity was found at 18-22 degreesc (154 +/- 62 ... | 1998 | 9705891 |
| a multidisciplinary approach using several biomarkers, including a parasite, as indicators of pollution: a case history from a paper mill in newfoundland. | several biomarkers are currently used to determine chronic exposure of fish to environmental contaminants. in studies to assess the effects of pulp mill effluent on winter flounder (pleuronectes americanus), body and hepatosomatic indices (hsis), histopathology, levels of hepatic detoxifying enzymes, mixed function oxygenases (mfo), muscle acetylcholinesterase (ache) and selected parasite abundance were compared in samples taken near a paper mill (sulphite-bleaching) and a number of reference si ... | 1997 | 9802066 |
| molecular cloning and characterization of two novel human renal organic anion transporters (hoat1 and hoat3). | the cloned organic anion transporters from rat, mouse, and winter flounder (roat1, moat1, froat) mediate the coupled exchange of alpha-ketoglutarate with multiple organic anions, including p-aminohippurate (pah). we have isolated two novel gene products from human kidney which bear significant homology to the known oats and belong to the amphiphilic solute facilitator (asf) family. the cdnas, hoat1 and hoat3, encode for 550- and 568-amino-acid residue proteins, respectively. hoat1 and hoat3 mrna ... | 1999 | 10049739 |
| expression cloning and characterization of a novel sodium-dicarboxylate cotransporter from winter flounder kidney. | a cdna coding for a na+-dicarboxylate cotransporter, fnadc-3, from winter flounder (pseudopleuronectes americanus) kidney was isolated by functional expression in xenopus laevis oocytes. the fnadc-3 cdna is 2384 nucleotides long and encodes a protein of 601 amino acids with a calculated molecular mass of 66.4 kda. secondary structure analysis predicts at least eight membrane-spanning domains. transport of succinate by fnadc-3 was sodium-dependent, could be inhibited by lithium, and evoked an inw ... | 1999 | 10400635 |
| secretory expression and site-directed mutagenesis studies of the winter flounder skin-type antifreeze polypeptides. | winter flounder contains both liver-type, extracellular antifreeze polypeptides (wflafps) and less active skin-type, intracellular antifreeze polypeptides (wfsafps). the lower activity of wfsafps might be due to their lack of complete ice-binding motifs '-k-dt-'. in order to test the functional role of this putative ice-binding motif, mutations were introduced into the n-terminal or c-terminal regions of wfsafp-2, which lack any presumptive ice-binding motifs. the wild-type and mutant wfsafp-2 w ... | 1999 | 10447672 |
| electrophysiologic characterization of an organic anion transporter cloned from winter flounder kidney (froat). | the two-electrode voltage clamp technique was used to demonstrate translocation of p-aminohippurate (pah) and related compounds such as loop diuretics in xenopus laevis oocytes expressing the renal organic anion transporter from winter flounder kidney (froat). in froat-expressing oocytes, pah (0.1 mm) induced a depolarization of 4.2 +/- 0.4 mv and at a holding potential of -60 mv an inward current of -22.6 +/- 3.5 na. pah-induced current and the current calculated from [3h]-pah uptake were of si ... | 2000 | 10616835 |
| extracellular expression, purification, and characterization of a winter flounder antifreeze polypeptide from escherichia coli. | hplc6 is the major component of liver-type antifreeze polypeptides (afps) from the winter flounder, pleuronectes americanus. to facilitate mutagenesis studies of this protein, a gene encoding the 37-amino acid mature polypeptide was chemically synthesized and cloned into the tac cassette immediately after the bacterial ompa leader sequence for direct excretion of the afp into the culture medium. escherichia coli transformant with the construct placiqpar8af was cultured in m9 medium. the recombin ... | 2000 | 10686148 |
| characterization of a fish antimicrobial peptide: gene expression, subcellular localization, and spectrum of activity. | antimicrobial peptides are proposed to act as the first line of mucosal host defense by exerting broad-spectrum microbicidal activity against pathogenic microbes. pleurocidin, a new 25-residue linear antimicrobial peptide, was recently isolated from the skin secretions of winter flounder (pleuronectes americanus). the present study identifies the cdna and gene encoding pleurocidin. the pleurocidin gene comprises four exons. its upstream region demonstrates consensus binding sequences for transcr ... | 2000 | 10898673 |
| predator-prey relations between age-1+ summer flounder (paralichthys dentatus, linnaeus) and age-0 winter flounder (pseudopleuronectes americanus, walbaum): predator diets, prey selection, and effects of sediments and macrophytes. | laboratory experiments and weekly trammel net surveys in the navesink river, new jersey (usa) were used to examine the predator-prey interaction between age-1+ summer flounder (paralichthys dentatus) and age-0 winter flounder (pseudopleuronectes americanus). winter flounder (24-67 mm tl) were the dominant piscine prey of summer flounder (n=95, 252-648 mm tl) collected in trammel nets. we observed a temporal shift in summer flounder diets from sand shrimp (crangon septemspinosa) and winter flound ... | 2000 | 10958899 |
| the rat ortholog of the presumptive flounder antifreeze enhancer-binding protein is a helicase domain-containing protein. | the expression of winter flounder liver-type antifreeze protein (wflafp) genes is tissue-specific and under seasonal and hormonal regulation. the only intron of the major wflafp gene was demonstrated to be a liver-specific enhancer in both mammalian cell lines and flounder hepatocytes. element b, the core enhancer sequence, was shown to interact specifically with a liver-enriched transcription factor, ccaat/enhancer-binding protein alpha (c/ebpalpha), as well as a presumptive antifreeze enhancer ... | 2000 | 11106437 |
| isolation and characterization of skin-type, type i antifreeze polypeptides from the longhorn sculpin, myoxocephalus octodecemspinosus. | the antifreeze polypeptides (afps) are found in several marine fish and have been grouped into four distinct biochemical classes (type i-iv). recently, the new subclass of skin-type, type i afps that are produced intracellularly as mature polypeptides have been identified in the winter flounder (pleuronectes americanus) and the shorthorn sculpin (myoxocephalus scorpius). this study demonstrates the presence of skin-type afps in the longhorn sculpin (myoxocephalus octodecemspinosus), which produc ... | 2001 | 11136728 |
| cluster of genes encoding the major egg envelope protein of zebrafish. | the proteins of fish egg envelopes are encoded by genes that are closely related to the genes for human zona pellucida proteins. a cluster of three genes coding for an egg envelope protein was isolated from the zebrafish, danio rerio. the three genes, zp2a, zp2b, and zp2c, are located within an 11 kb region and are each comprised of eight exons spanning 1.85 kb. the exon-intron structures of the genes are nearly identical; however, their deduced amino acid sequences diverge at exon 7 (zp2b and z ... | 2001 | 11144219 |
| interaction of pleurocidin and its analogs with phospholipid membrane and their antibacterial activity. | a 25-mer cationic peptide pleurocidin, isolated from the winter flounder, has broad antibacterial activity. to clarify the structure-activity relationship, its properties and biological activity were examined. cd measurements showed that pleurocidin took an alpha-helical structure in the presence of dopc/dopg (3:1, anionic) vesicles. very weak hemolytic activity of pleurocidin was observed and its antibacterial activity was moderate. tryptophan fluorescence shift measurements showed that pleuroc ... | 2001 | 11168895 |
| tissue-level cytoprotection. | in vitro and ex vivo tissue models provide a useful level of biological organization for cytoprotection studies positioned between cultured cells and intact animals. we have used 2 such models, primary tissue cultures of winter flounder renal secretory epithelium and ex vivo preparations of rat intestinal tissues, the latter to access the microcirculation of exposed mesentery tissues. herein we discuss studies indicating that differentiated functions are altered in thermotolerant or cytoprotecte ... | 2000 | 11189445 |
| potential-dependent steady-state kinetics of a dicarboxylate transporter cloned from winter flounder kidney. | the two-electrode voltage-clamp technique in combination with tracer uptake experiments was used to investigate the dependence of dicarboxylate transport kinetics on membrane potential in xenopus laevis oocytes expressing the flounder renal high-affinity-type sodium dicarboxylate cotransporter (fnadc-3). steady-state succinate-dependent currents in the presence of na+ were saturable with an apparent affinity constant for succinate, k0.5,succ, of 60 microm. k0.5,succ was independent of membrane p ... | 2000 | 11211120 |
| structure, expression and activation of fish ras genes. | ras genes encode proteins that play a central role in cell growth signaling cascades. the fish ras genes characterized to date, have a high degree of nucleotide sequence and deduced amino acid similarity with the mammalian ras gene counterparts. a large proportion and wide variety of mammalian tumors possess mutant forms of ras. in such cases, the localization of ras mutations has been restricted to exons i and ii, and to codons 12, 13 and 61. experimental exposure of fish to a range of genotoxi ... | 2001 | 11551618 |
| flounder antifreeze peptides increase the efficacy of cryosurgery. | type i antifreeze protein (afp) from the winter flounder (pseudopleuronectes americanus) was used as an adjuvant to cryosurgery of subcutaneous tumors of dunning at-1 rat prostate cells grown in copenhagen rats. the cryosurgical procedure was performed with a commercially available cryosurgery device (cryo-hit, galil medical) with clinically relevant single- and double-freeze protocols. injury was assessed with the alamar blue indicator of metabolic activity. the assay gave anomalous results whe ... | 2001 | 11578117 |
| 3,3',4,4'-tetrachlorobiphenyl oxidation in fish, bird and reptile species: relationship to cytochrome p450 1a inactivation and reactive oxygen production. | previously we showed that the polychlorinated biphenyl 3,3',4,4'-tetrachlorobiphenyl (tcb) caused a release of reactive oxygen species (ros) from cytochrome p450 1a (cyp1a) of the fish scup (stenotomus chrysops), and from rat and human cyp1a1. this was linked to a tcb- and nadph-dependent oxidative inactivation of the enzyme, which in scup and rat was inversely related to the rates of tcb oxidation. we examined the relationship between rates of tcb oxidation, cyp1a inactivation and ros productio ... | 2000 | 11790349 |
| risk based management of contaminated sediments: consideration of spatial and temporal patterns in exposure modeling. | this paper addresses interactions among foraging behavior, habitat preferences, site characteristics, and spatial distribution of contaminants in developing pcb exposure estimates for winter flounder at a hypothetical open water dredged material disposal site in the coastal waters of new york and new jersey (ny-nj). the implications of these interactions for human health risk estimates for local recreational anglers who fish for and eat flounder are described. the models implemented in this stud ... | 2002 | 11827058 |
| the use of spatial modeling in an aquatic food web to estimate exposure and risk. | this paper quantitatively evaluates interactions among foraging behavior, habitat preferences, site characteristics and the spatial distribution of contaminants in estimating pcb exposure concentrations for winter flounder at a hypothetical open water dredged material disposal site in the coastal waters of new york and new jersey (ny-nj). the models implemented in this study include a spatial submodel to account for spatial and temporal characteristics of fish exposure and a probabilistic adapta ... | 2002 | 12013551 |
| first report of piscine nodavirus infecting wild winter flounder pleuronectes americanus in passamaquoddy bay, new brunswick, canada. | piscine nodaviruses (betanodaviridae) are frequently reported from a variety of cultured and wild finfishes. these non-enveloped, single-stranded rna virions cause viral encephalopathy and retinopathy (ver), also known as viral nervous necrosis (vnn) or fish encephalitis. recently, nodavirus infections have posed serious problems for larval and juvenile cultured halibut hippoglossus hippoglossus in norway and scotland. to date, no such viruses have been described from any cultured or wild pleuro ... | 2002 | 12078988 |
| polyvalent cation receptor proteins (cars) are salinity sensors in fish. | to determine whether calcium polyvalent cation-sensing receptors (cars) are salinity sensors in fish, we used a homology-based cloning strategy to isolate a 4.1-kb cdna encoding a 1,027-aa dogfish shark (squalus acanthias) kidney car. expression studies in human embryonic kidney cells reveal that shark kidney senses combinations of ca(2+), mg(2+), and na(+) ions at concentrations present in seawater and kidney tubules. shark kidney is expressed in multiple shark osmoregulatory organs, including ... | 2002 | 12093923 |
| regulation of the npt gene by a naturally occurring antisense transcript. | the epithelial na/pi cotransporter (napi-ii) is instrumental in maintaining phosphate (pi) homeostasis in vertebrates. hormones and metabolic factors (pth, pi availability) that acutely influence renal pi excretion have been demonstrated to target napi-ii expression. upon stimulation, newly synthesized transporter molecules become integrated into the brush-border membrane to increase the vmax of pi uptake; reduction of pi reabsorption is achieved by endocytosis of napi-ii followed by lysosomal d ... | 2002 | 12139410 |
| transport of cimetidine by flounder and human renal organic anion transporter 1. | the h(2)-receptor antagonist cimetidine is efficiently excreted by the kidneys. in vivo studies indicated an interaction of cimetidine not only with transporters for basolateral uptake of organic cations but also with those involved in excretion of organic anions. we therefore tested cimetidine as a possible substrate of the organic anion transporters cloned from winter flounder (froat) and from human kidney (hoat1). uptake of [(3)h]cimetidine into froat-expressing xenopus laevis oocytes exceede ... | 2003 | 12429554 |
| health of flatfish from localities in placentia bay, newfoundland, contaminated with petroleum and pcbs. | this study compared the health of flatfish inhabiting two areas in placentia bay, newfoundland, contaminated either with polycyclic aromatic hydrocarbons (pahs) or polychlorinated biphenyls (pcbs) with samples from reference sites. initially, four species of flatfish, including american plaice (hippoglossoides platessoides), yellowtail flounder (limanda ferruginea), gray sole (glyptocephalus cynoglossus), and winter flounder (pleuronectes americanus), were sampled only for external lesions from ... | 2003 | 12712279 |
| aqueous extracts from some muscles inhibit hemoglobin-mediated oxidation of cod muscle membrane lipids. | it was evaluated whether trout hemoglobin (hb)-mediated oxidation of minced washed cod muscle lipids could be prevented by an aqueous isolate from cod and some other muscle sources. lipid hydroperoxides and painty odor developed approximately 4 days faster in washed than unwashed cod mince. when adding back an aqueous fraction (press juice) isolated from unwashed mince to washed mince at 2-6-fold dilutions, development of hydroperoxides and painty odor was either delayed or completely prevented. ... | 2003 | 12720401 |
| molecular cloning of nhe1 from winter flounder rbcs: activation by osmotic shrinkage, camp, and calyculin a. | in this report, we describe the cloning, cellular localization, and functional characteristics of na(+)/h(+) exchanger 1 (nhe1) from red blood cells of the winter flounder pseudopleuronectes americanus (panhe1). the panhe1 protein localizes primarily to the marginal band and exhibits a 74% similarity to the trout beta-nhe, and 65% to the human nhe1 (hnhe1). functionally, panhe1 shares characteristics of both beta-nhe and hnhe1 in that it is activated both by manipulations that increase camp and ... | 2003 | 12734109 |
| basolateral localization of flounder na+-dicarboxylate cotransporter (fnadc-3) in the kidney of pleuronectes americanus. | the purpose of this study was to provide functional and immunocytochemical evidence for the location of the winter flounder ( pleuronectes americanus) sodium-dicarboxylate cotransporter-3 (fnadc-3) in the basolateral membrane of proximal tubule cells. fnadc-3 was expressed in xenopus laevis oocytes. lowering the external ph from 7.5 to 6.5 or 5.5 modestly decreased the uptake of [(14)c]succinate into fnadc-3 expressing oocytes, but markedly increased the uptake of [(14)c]citrate. as measured by ... | 2003 | 12759753 |
| novel antimicrobial peptides derived from flatfish genes. | we report on the identification of active novel antimicrobials determined by screening both the genomic information and the mrna transcripts from a number of different flatfish for sequences encoding antimicrobial peptides, predicting the sequences of active peptides from the genetic information, producing the predicted peptides chemically, and testing them for their activities. we amplified 35 sequences from various species of flatfish using primers whose sequences are based on conserved flanki ... | 2003 | 12878506 |
| cortisol alters carbonic anhydrase-mediated renal sulfate secretion. | active transepithelial sulfate secretion rate by winter flounder renal proximal tubule epithelium in primary culture (fptc) is dependent on intracellular carbonic anhydrase (ca) and enhanced by cortisol. to further evaluate this relationship, a partial cdna clone (327 bp) of carbonic anhydrase ii (caii) with high sequence similarity to caii from numerous species including fish, chicken, and human was obtained from fptcs. the majority of ca activity and caii protein was present in the cytosol of ... | 2003 | 12907415 |
| identification, structure and differential expression of novel pleurocidins clustered on the genome of the winter flounder, pseudopleuronectes americanus (walbaum). | antimicrobial peptides form one of the first lines of defense against invading pathogens by killing the microorganisms and/or mobilizing the host innate immune system. although over 800 antimicrobial peptides have been isolated from many different species, especially insects, few have been reported from marine fish. sequence analysis of two genomic clones (15.6 and 12.5 kb) from the winter flounder, pseudopleuronectes americanus (walbaum) resulted in the identification of multiple clustered gene ... | 2003 | 12950255 |
| characterization of aquabirnaviruses from flounder pseudopleuronectes americanus and mummichog fundulus heteroclitus in the chesapeake bay, virginia, usa. | viruses were isolated in cell culture from tissue homogenates of flounder pseudopleuronectes americanus and mummichog fundulus heteroclitus in the chesapeake bay, virginia, usa. neutralization and immunofluorescence tests with aquabirnavirus (west buxton strain)-specific polyclonal antisera indicated that both viruses were aquabirnaviruses belonging to serogroup a, the most common aquabirnavirus serogroup in the united states. this was confirmed by rt-pcr, with primers targeting the vp3 and vp2 ... | 2003 | 14667031 |
| effect, distribution, and prevalence of glugea stephani (microspora) in winter flounder (pleuronectes americanus) living near two pulp and paper mills in newfoundland. | a study was conducted to determine the effects, geographical distribution, and prevalence of a microsporan parasite, glugea stephani, in winter flounder (pleuronectes americanus) in newfoundland. fish were captured by scuba divers in several coastal areas, including 2 embayments where pulp and paper mill effluent was discharged, as well as a number of pristine sites. fish health was assessed by comparing histological profiles, condition factors (k), organosomatic indices and blood values between ... | 2004 | 15165042 |
| interactions of benzylpenicillin and non-steroidal anti-inflammatory drugs with the sodium-dependent dicarboxylate transporter nadc-3. | sodium-dependent dicarboxylate transporters located in the basolateral membrane (nadc-3) of renal proximal tubule cells maintain the driving force for exchange of organic anions and drugs against alpha-ketoglutarate via organic anion transporters oat1 and oat3. so far, information on direct interaction of drugs with the cloned nadc-3 was missing. here we tested the interaction of non-steroidal anti-inflammatory drugs (nsaids) and benzylpenicillin with nadc-3 cloned from winter flounder (fnadc-3) ... | 2004 | 15319545 |
| suppression subtractive hybridization cdna libraries to identify differentially expressed genes from contrasting fish habitats. | suppression subtractive hybridization complementary dna libraries identified differentially expressed genes in liver tissue of winter flounder collected from the highly impacted raritan-hudson estuary versus those from less industrialized estuaries farther south in new jersey. distinct transcript profiles emerged in the fish from these different habitats. a total of 251 clones from the forward (upregulated with anthropogenic impact) and reverse (downregulated with anthropogenic impact) subtracte ... | 2004 | 15546050 |
| transport of the natural sweetener stevioside and its aglycone steviol by human organic anion transporter (hoat1; slc22a6) and hoat3 (slc22a8). | the natural sweetening agent stevioside and its aglycone metabolite, steviol, have been shown to inhibit transepithelial transport of para-aminohippurate (pah) in isolated rabbit renal proximal tubules by interfering with basolateral entry. the aim of the present study was to determine which of the cloned basolateral organic anion transporters were involved in the renal transport of stevioside and steviol. this question was addressed in xenopus laevis oocytes expressing human organic anion trans ... | 2005 | 15644426 |
| effects of nonylphenol on hepatic testosterone metabolism and the expression of acute phase proteins in winter flounder (pleuronectes americanus): comparison to the effects of saint john's wort. | 4-nonylphenol (4-np), a major by-product of alkylphenol ethoxylates, is used in several industries and as a consequence is quite common in rivers, estuaries and other aquatic environments that receive sewage discharges or are near offshore oil platforms. 4-np is an environmental estrogen that also binds human and rodent pregnane x-receptor (pxr), the orphan nuclear receptor that controls the expression of several detoxication genes in mammals, including several cyp3a and cyp2b family members. th ... | 2005 | 15792627 |
| a c-terminal glycine suppresses production of pleurocidin as a fusion peptide in escherichia coli. | the winter flounder (pseudopleuronectes americanus) antimicrobial peptide pleurocidin was produced in escherichia coli using a synthetic gene constructed by pcr. the gene expresses pleurocidin from pet21a fused to the c-terminus of an insoluble carrier peptide. once expressed, the fusion peptide formed inclusion bodies in the cytoplasm that were collected, solubilized in guanidine-hcl, and chemically cleaved using hydroxylamine at a unique asparaginyl-glycyl dipeptide. this released recombinant ... | 2006 | 15935695 |
| assessment of stress-related bioindicators in winter flounder (pleuronectes americanus) exposed to discharges from a pulp and paper mill in newfoundland: a 5-year field study. | this study assessed the effects of discharges from a sulphite-bleaching paper mill on winter flounder (pleuronectes americanus) sampled each spring over a 5-year period in st. george's bay, newfoundland, prior to foraging activity. the fish were captured by scuba divers near the mill and at a reference site 10 km up current. several bioindicators were used to assess fish health. larger and older flounder exhibiting gross and microscopic tissue lesions, lower condition factor, and elevated hepato ... | 2006 | 16485169 |