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pollution of the sea and its effects.marine pollution has been studied under the following groupings of effects; harm to living resources, hazards to human health, reduction of amenities and interference with other users of the sea. this paper is concerned mainly with the first two categories and their interrelation. apart from certain seabirds affected by oil, the major stocks of marine animals show no evidence of reduction by pollution. pollution effects are generally insignificant in relation to other factors governing reproduct ...197940238
phagocytosis by the endocardial lining cells of the atrium of plaice (pleuronectes platessa). 1975171287
the immune response of plaice (pleuronectes platessa l) to the metacercariae of cryptocotyle lingua and rhipidocotyle johnstonei.the humoral immune response of plaice to infestations of selected metacercariae was studied. precipitating antibodies to both cryptocotyle lingua and rhipidocotyle johnstonei were demonstrated using agar gel diffusion techniques. immunochemical investigations of these antibodies indicated they were macroglobulins, resembling immunoglobulin m (igm) of mammals. the rate and magnitude of antibody production by the host was determined by the ambient temperature.1977402627
fluorescent histochemistry of the teleost gut: evidence for the presence of serotonergic neurones.the guts of the teleosts myoxocephalus scorpius, pleuronectes platessa and clupea harengus were examined using fluorescent histochemistry and the innervation compared to that in mammals and other lower vertebrates. in myoxocephalus and pleuronectes both greeen and yellow fast fading fluorescent nerves were found in the intestine. to test whether the yellow nerves might be serotonergic, an attempt was made to extract 5-ht from the intestine of these fish in a region where enterochromaffin cells w ...1979455398
increased extracellular production of a cholinesterase-solubilising factor by cytophaga ncmb 1314 during magnesium starvation.a cytophaga sp. with the property of liberating a cholinesterase which is found in body muscle of plaice was studied. the liberation was caused by a factor of which more than 90% was found outside the bacterial cell and might possibly be associated within the slime material surrounding the bacteria. magnesium limitation during growth of cytophaga sp. in batch cultures resulted in an about 10-fold increase in extracellular factor activity. the increase could be immediately stopped by addition of ...1978638892
the experimental pathogenesis of focal tuberculosis in the plaice (pleuronectes platessa l.). 1977838906
the presence of a prostaglandin-like substance in the skin of the plaice, pleuronectes plastessa l [proceedings]. 1977912188
studies on the nutrition of marine flatfish. the metabolism of glucose by plaice (pleuronectes platessa) and the effect of dietary energy source on protein utilization in plaice.1. the effects of dietary energy level and dietary energy source on protein utilization by plaice (pleuronectes platessa) were examined by giving diets containing 400 g crude protein/kg to nine groups of fish. five of these diets contained only lipid as a source of energy (in addition to protein) and their energy contents were varied by increasing the lipid level in a step-wise manner from 56 to 176 g/kg. the remaining four diets contained both lipid and carbohydrate (glucose plus dextrin) toget ...19751115761
cytoskeleton of podocytes in vertebrate animals and human.using tem and immunofluorescence microscope, a study was made on podocytes in vertebrates where an intermediate-sized filament system is replaced by a microtubule system, accompanied by highly developed microfilaments structures. a comparative immunofluorescence study was carried out on cryotome renal sections of plaice (pleuronectes platessa l.) and rats, using specific antibodies anti-cytokeratins and anti-vimentin. with polyclonal anti-vimentin serum the capillaries of the renal glomeruli sho ...19921282425
multiplicity of udp-glucuronosyltransferases in fish. purification and characterization of a phenol udp-glucuronosyltransferase from the liver of a marine teleost, pleuronectes platessa.the aim of this work was to determine if a non-mammalian species had multiple udp-glucuronosyltransferase (udpgt) isoforms. at least six highly purified udpgt isoenzymes were partially resolved by anion-exchange chromatography and udp-hexanolamine-sepharose 4b affinity chromatography from liver microsomes of a fish, the plaice. q-sepharose ff, chromatofocusing and affinity-chromatographic procedures were employed to separate and purify the phenol udpgt isoform to apparent homogeneity. the purifi ...19921599427
functional and immunochemical comparison of hepatic udp-glucuronosyltransferases in a piscine and a mammalian species.1. the aglycone specificity of hepatic microsomal glucuronidation was compared under uniform conditions in a fish, pleuronectes platessa and a mammal, rattus norvegicus, representative of the most primitive and advanced vertebrate classes. 2. both species exhibited comparable udp-glucuronosyltransferase (udpgt) activity towards planar phenolic substrates (1-naphthol, 4-nitrophenol); however, plaice activity towards bulky non-planar substrates such as (-)-morphine was either 200-fold lower, or fo ...19921617946
differential expression and induction of udp-glucuronosyltransferase isoforms in hepatic and extrahepatic tissues of a fish, pleuronectes platessa: immunochemical and functional characterization.glucuronidation of three substrates prototypical for different udp-glucuronosyltransferase (udpgt) isoforms in hepatic, renal, intestinal, and branchial microsomes of corn oil, 3-methylcholanthrene, aroclor 1254, and clofibrate-pretreated plaice was investigated. the differential expression of udpgt in the four tissues clearly demonstrated for the first time that multiple isoforms with differing substrate specificities were present in fish. the liver was quantitatively the most important site fo ...19921631888
environmental contaminants and biochemical responses in flatfish from the hvaler archipelago in norway.the levels of several environmental contaminants, including selected polyaromatic hydrocarbons (pah), organochlorines (ddt/dde, hexachlorobenzene), 15 polychlorinated biphenyl (pcb) congeners, and polychlorinated dibenzofurans and dibenzo-p-dioxins, pcdf/pcdd), and heavy metals (cd, hg, pb, and as) were analyzed in muscle and liver of three different flatfish species (dab, limanda limanda; flounder, platichthys flesus; plaice, pleuronectes platessa) caught by gill netting at different sites in t ...19911759844
effects of steroids and human chorionic gonadotrophin on in vitro oocyte final maturation in two marine flatfish: the dab, limanda limanda, and the plaice, pleuronectes platessa.this study investigated the role of gonadotrophin and steroids in the induction of in vitro oocyte maturation in dab (limanda limanda) and plaice (pleuronectes platessa), two marine flatfish (pleuronectiformes) in which the oocytes mature and ovulate in groups (or batches). clusters of folliculated oocytes were incubated for 3 days with a range of doses of either human chorionic gonadotrophin (hcg) or synthetic steroids with a wide range of structures. they were also incubated with extracts from ...19902307340
immunological distinction between piscine and mammalian metallothioneins.1. separate antisera to metallothioneins (mt) from rainbow trout and horse were produced in mice and their reactivity with the respective immunogen was confirmed using an elisa. 2. the elisa, used in a competitive mode, revealed that the anti-horse mt serum did not cross-react with trout mt. reciprocally, the anti-trout mt serum did not show any reactivity with horse mt. 3. the anti-rainbow trout mt serum was shown to cross-react totally with mts from plaice, flounder, turbot, perch, salmon and ...19902331881
identification of, and development of radioimmunoassays for 17 alpha, 21-dihydroxy-4-pregnene-3,20-dione and 3 alpha,17 alpha, 21-trihydroxy-5 beta-pregnan-20-one in the ovaries of mature plaice (pleuronectes platessa).ovaries from a female plaice (pleuronectes platessa) that had been injected with human chorionic gonadotrophin were incubated in vitro with 17 alpha-hydroxy[1,2,6,7-3h]progesterone. the major steroids produced by the ovaries were tentatively identified as 17 alpha,21-dihydroxy-4-pregnene-3,20di-one (11-deoxycortisol; 17,21-p), 17 alpha,21-dihydroxy-5 beta-pregnane-3,20-dione (3 alpha, 17,21-p-5 beta). a high proportion of these steroids was found in a conjugated form (sulphates or glucuronides). ...19902354767
plasma levels of ovarian steroids, including 17 alpha,21-dihydroxy-4-pregnene-3,20-dione and 3 alpha,17 alpha,21-trihydroxy-5 beta-pregnan-20-one, in female plaice (pleuronectes platessa) induced to mature with human chorionic gonadotrophin.in one previous paper we reported on the identification of 11-deoxycortisol and 3 alpha,17 alpha,21-trihydroxy-5 beta-pregnan-20-one in the ovaries and plasma of mature female plaice and also described the development of radioimmunoassays for these two steroids. the present paper describes temporal changes in plasma levels of the free and conjugated forms of these and of some other steroids (17 alpha,20 beta-dihydroxy-4-pregnen-3-one, 17 alpha,20 beta,21-trihydroxy-4-pregnen-3-one, 17 alpha,20 a ...19902354768
comparison of the chitinolytic properties of clostridium sp. strain 9.1 and a chitin-degrading bacterium from the intestinal tract of the plaice, pleuronectes platessa (l.).the chitinolytic properties of a facultatively anaerobic bacterium isolated from the hindgut of plaice were compared with those of clostridium sp. strain 9.1, a bacterium isolated from anoxic estuarine sediment. the chitinolytic enzyme systems of the gut isolate and strain 9.1 both released n,n'-diacetylchitobiose (nag2) as the major hydrolysis end-product. during the hydrolysis of chitin, there was transient accumulation of a non-sedimentary chitin fraction which was not detectable by high-perf ...19902398346
degradation of human epidermal keratin by fish pepsin.stomach extract of atlantic herring clupea harengus, atlantic salmon salmo salar, cod gadus morhua, redfish sebastes marinus, and plaice pleuronectes platessa, degraded human epidermal keratin effectively in vitro. the keratin-degrading activity of all extracts showed a ph optimum around 3.3-3.4, and sheets of plantar callus were degraded with about the same efficacy as keratin. pepstatin sensitivity, heat lability, and the acidic ph optimum demonstrated that the keratin-degrading activity was p ...19882456043
[immunomorphologic study of vertebrate renal glomerula using antibodies to intermediate filament proteins].a comparative immunomorphologic study was carried out on cryostate sections of renal tissue of plaice (pleuronectes platessa l.) and rat, using specific antibodies against the proteins of intermediate filaments--cytokeratins and vimentin. no cytokeratins were revealed in cells of renal glomerula in both the animals under investigation. indirect immunofluorescence of the polyclonal serum against vimentin showed brightly coloured capillaries of the renal glomerula of plaice and weakly coloured one ...19892474215
characterization of the repetitive dna elements in the genome of fish lymphocystis disease viruses.the complete dna nucleotide sequence of the repetitive dna elements in the genome of fish lymphocystis disease virus (fldv) isolated from two different species (flounder and dab) was determined. the size of these repetitive dna elements was found to be 1413 bp which corresponds to the dna sequences of the 5' terminus of the ecori dna fragment b (0.034 to 0.052 m.u.) and to the ecori dna fragment m (0.718 to 0.736 m.u.) of the fldv genome causing lymphocystis disease in flounder and plaice. the d ...19892773322
the influence of hormones and inflammatory agents on c-reactive protein, cortisol and alanine aminotransferase in the plaice (pleuronectes platessa l.).endotoxin stimulates production of both c-reactive protein (crp) and cortisol in the plaice within 24 hr. cortisol alone (optimum dose i.p. 500 micrograms/300 g wt fish) also stimulates crp production and the possibility that endotoxin acts through cortisol was examined. dexamethasone suppresses cortisol production but elevates crp. cortisol levels are restored to normal within 24 hr of endotoxin injection. turpentine and acth which stimulate cortisol do not affect crp. endotoxin and cortisol ha ...19852858357
isolation of substances with vascular permeability-increasing activity from the skin of the plaice (pleuronectes platessa l.).a methanolic extract of plaice skin, from which lipids had been removed, was chromatographed on alumina, eluted with decreasing concentrations of ethanol. only the 60% ethanol fraction exhibited smooth muscle activity, with bradykinin-like properties. the 20% ethanol fraction increased vascular permeability in rat skin, as measured by dye-leakage. this was not due to the degranulation of mast cells. intradermal injection of either fraction into the plaice caused localized erythema.19852861058
transfer of passive immunity from mother to young in a teleost fish: haemagglutinating activity in the serum and eggs of plaice, pleuronectes platessa l.plaice were immunized against rabbit red blood cells (rbc) and the haemagglutinating activity in their sera and eggs was isolated by gel filtration in sephacryl s300 sf. a "heavy" (greater than 6.69 x 10(5) daltons) and a "light" (less than 2.32 x 10(5) daltons) component of haemagglutinating activity were present in plaice immune serum and eggs. the "heavy" haemagglutinating component had the characteristics of immunoglobulin m (igm) antibody. by contrast the "light" component was suppressed gr ...19862873930
effect of high hydrostatic pressure on 86rb+ influx in the erythrocyte of the plaice (pleuronectes platessa).1. 86rb+ influx in the erythrocyte of the plaice (pleuronectes platessa) has been measured at hydrostatic pressures between 1 and 600 atm at 10 degrees c. 2. the measurements were performed with an experimental medium containing 1% (w/v) bovine serum albumin. in this medium the cells achieved a steady state level of ionic regulation. 3. at normal atmospheric pressure 46% of the 86rb+ influx was inhibited by furosemide while 42% was inhibited by ouabain, the remainder being inhibited by neither d ...19872892621
comparative histochemistry of a flatfish fin muscle and of other vertebrate muscles used for ultrastructural studies.because of the high degree of filament order in the myofibrils of fish skeletal muscles, and the resulting usefulness of such preparations (particularly flatfish fin muscles) in structural studies of muscular contraction, the fibre type composition of plaice fin muscle has been determined by conventional histochemical tests. as controls, and for comparison, fibre type distributions have also been studied in several other vertebrate skeletal muscles which are widely used for ultrastructural and m ...19872958501
nmr studies of crab and plaice metallothioneins.metallothioneins isolated from the hepatopancreas of the edible crab (cancer paqurus) and the plaice (pleuronectes platessa) after cadmium injection are predominantly cadmium proteins containing only small amounts of zinc and traces of copper. the removal of metal ions from the two metallothioneins by edta was studied using proton nmr spectroscopy. the rates of removal of cadmium and zinc were monitored directly from the intensity of the resonances due to the cadmium and zinc-edta complexes. nea ...19863086076
immunochemical cross-reactions between pentraxins of different species.monospecific antisera were raised by immunization with pentraxins, c-reactive protein (crp) or serum amyloid p component (sap), that had been isolated from man and a number of different vertebrate species. these antisera were used to test sera or serous fluids from a range of other vertebrate species and also some invertebrates. cross-reactions between species were comparatively rare but were seen among primates and among ruminants. there was also cross-reactivity between these two groups. antis ...19873115892
[spectral analysis of the cardiac rhythm of fishes].spectral analysis of the rhythmograms in the cod gadus morhua callarias, plaice pleuronectes platessa, herring clupea harengus membras and trout salmo gairdneri revealed complex wave structure of their cardiac rhythm. in all the species investigated, slow (0.01-0.06 hz), intermediate (0.06-0.25 hz) and fast (0.25-0.5 hz) waves were recorded. it is suggested that the wave structure of cardiac rhythm reflects rather complicated relationships between functional systems regulating cardiac activity.19873673370
urinary excretion of 1-methylhistidine: a qualitative indicator of exogenous 3-methylhistidine and intake of meats from various sources.in order to investigate whether the urinary excretion of 1-methylhistidine (1mh) might serve as an objective indicator of meat ingestion and exogenous 3-methylhistidine (3mh) intake, healthy subjects were fed an ovolactovegetarian diet. at five-day intervals they were given meat of different origin and 24-hour urinary excretions of 1mh and 3mh were determined. after beef intake there was a marked increase of 3mh and 1mh excretion. the elimination curves were found to follow first-order kinetics ...19873683186
analysis of the structure of fish lymphocystis disease virions from skin tumours of pleuronectes.virions of fish lymphocystis disease (fldv) from tumour-carrying-fishes (flounder, dab, plaice and gurnard) collected in the north sea were isolated directly from the tumours and purified by sucrose and subsequent caesium chloride gradient centrifugation. they were studied by electron microscopy using embedding methods, negative staining and using metal shadowing methods. tumours of dermal connective fish tissue showed particles with hexagonal outlines. occasionally, an "empty structure" was obs ...19863753198
migration of fish leucocytes in vitro: the effect of factors which may be involved in mediating inflammation.plaice (pleuronectes platessa l.) neutrophils were isolated from the kidney on a discontinuous percoll gradient and from the peritoneal cavity at the peak of a glycogen-elicited inflammatory response. the migratory ability of neutrophils was assessed using a 48-well microchemotaxis chamber, with an incubation of 1.5 h at 12 degrees c. the two neutrophil populations showed different responses to n-formylmethionyl-leucyl-phenylalanine (fmlp). whereas kidney neutrophils only showed a significant en ...19863765366
concentrations of arsenic in urine of the general population in sweden.the concentration of the sum of the metabolites of inorganic arsenic (inorganic arsenic, methylarsonic acid and dimethylarsinic acid) as well as the concentration of organic arsenic compounds, mainly in the form of arsenobetaine, in the urine of human subjects from two cities in sweden have been studied. the median concentration of metabolites of inorganic arsenic was approximately 8 micrograms as/g creatinine, independent of place of residence, sex, smoking and consumption of beer and wine. how ...19863810123
effect of opsonization on oxidative metabolism of plaice (pleuronectes platessa l.) neutrophils.the effect of serum opsonization on vibrio alginolyticus (heat-killed)-stimulated chemiluminescence (cl) by plaice kidney- and peritoneal exudate-derived neutrophils was investigated. peritoneal neutrophils only recognized heat-labile and kidney neutrophils only heat-stable opsonic activity in normal serum. specific antibody did not show opsonic activity nor any synergism with the normal serum opsonins for either neutrophil population. evidence was found for the production, by plaice neutrophils ...19873829631
organochlorine pesticides and polychlorinated biphenyls in flatfish from the southern baltic, 1983.hexachlorobenzene (hcb), alpha-, beta-, gamma- and delta-benzenehexachloride (bhc; hch), p,p'-dde, o,p'-ddd, o,p'-ddt, p,p'-ddd and p,p'-ddt (espilon ddt) and polychlorinated biphenlys (pcbs) levels have been determined in muscle tissue of 37 turbots psetta maxima, 27 plaices pleuronectes platessa and 106 flounders platichthys flesus netted during 1983 in a different regions in the southern part of the baltic sea. the mean levels found for flatfish muscle tissue related to wet weight were: 0.7, ...19853934867
trace metals in flatfish from the southern baltic, 1983.the cadmium, lead, copper, zinc, iron, manganese, and nickel concentrations have been determined in muscle tissue of 35 turbot psetta maxima, 27 plaice pleuronectes platessa and 103 flounders platichthys flesus netted in 1983 in a different regions in the southern part of the baltic sea. the method of measurement was atomic-absorption spectrophotometry. the mean values obtained for turbot, plaice and flounder were 0.012, 0.009 and 0.013 for cadmium, 0.16, 0.17 and 0.12 for lead, 0.15, 0.14 and 0 ...19854050125
conjugation of organic compounds in isolated hepatocytes from a marine fish, the plaice, pleuronectes platessa.a method is described for the preparation of viable hepatocytes from a marine fish, the plaice. their ability to detoxify organic compounds was measured by the formation of glucuronic acid and sulphate conjugates with the model substrates 1-naphthol and phenolphthalein. 1-naphthol was conjugated three- to four-fold faster than phenolphthalein and glucuronidation predominated with both substrates. strong substrate inhibition of glucuronidation was observed with 200 microm 1-naphthol or phenolphth ...19854062967
c-reactive protein-like precipitins in plaice. 19734148473
a bacterial factor capable of solubilizing cholinesterase from plaice body muscle. 19684305082
studies on crystalline lactate dehydrogenase from cardiac and skeletal muscle of plaice (pleuronectes platessa) with particular reference to temperature. 19695354259
purification of a cholinesterase from the body muscles of plaice (pleuronectes platessa). 19675585682
the solubilization of a cholinesterase from plaice muscle by bacteria. 19665941307
distribution of endotoxin and its effect on serum concentrations of c-reactive protein and cortisol in the plaice (pleuronectes platessa l.).bacterial endotoxins cause a significant increase in the serum concentration of both c-reactive protein (crp) and cortisol in the plaice. the time course of the crp elevation varies with the bacterial source, method of isolation of the endotoxin and the route of administration. injection of the prostaglandin synthetase inhibitor, indomethacin, 2 hr before endotoxin, abolishes the increases in both crp and cortisol, whereas it has no effect when given 6 hr after. using [51cr]labeled endotoxin, 40 ...19846149886
evolution of human alpha 2-macroglobulin.a papain-binding protein (pbp) resembling human alpha 2-macroglobulin (alpha 2m) but of mr half that of alpha 2m was purified from plaice (pleuronectes platessa l.) plasma. the plaice protein displayed most of the distinctive inhibitory properties of the human macroglobulin, and was therefore considered, despite its smaller molecular size, to be homologous with alpha 2m. plaice pbp was shown to consist of four dissimilar subunits; two i chains (mr 105 000) and two ii chains (mr 90 000). each of ...19816177138
evolution of alpha 2-macroglobulin. the structure of a protein homologous with human alpha 2-macroglobulin from plaice (pleuronectes platessa l.) plasma.the plaice (pleuronectes platessa l.) papain-binding protein previously demonstrated to be homologous with human alpha(2)-macroglobulin, and designated plaice alpha(2)-macroglobulin homologue or alphamh, was shown to be a glycoprotein of s(20,w) 11.86s. in polyacrylamide-gel pore-limit electrophoresis under non-denaturing conditions plaice alphamh migrated to the same position as half-molecules of human alpha(2)-macroglobulin, and treatment with methylamine or a proteinase caused no change in it ...19826181776
evolution of alpha 2-macroglobulin. the purification and characterization of a protein homologous with human alpha 2-macroglobulin from plaice (pleuronectes platessa l.) plasma.a papain-binding protein (pb-protein) was purified to homogeneity from the plasma of plaice (pleuronectes platessa l.). pb-protein inhibited the activity of trypsin and pancreatic elastase (serine proteinases), thermolysin (a metalloproteinase) and papain (a cysteine proteinase). presaturation of pb-protein with trypsin prevented the subsequent inhibition of thermolysin, and vice versa. only catalytically active endopeptidases were bound by pb-protein. the catalytic activity of trypsin bound by ...19826181779
the effect of natural and artificial light via the eye on the hormonal and metabolic balance of animal and man.color change of the plaice and frog, and development of the testes in duck are induced by light stimuli via the eye. eliminating experiences on the frog (hollwich) and on the duck (benoit) showed that these stimuli-beside vision-use a separate way, the so-called 'energetic portion' of the visual pathway (hollwich). in compared studies on blind and on temporarily blind patients involved by cataract in both eyes, we found significant low levels of acth and cortisol. the levels normalized after cat ...19806255392
rare variant alleles in the light of the neutral theory.based on the neutral theory of molecular evolution and polymorphism, and particularly assuming "the model of infinite alleles," a method is proposed which enables us to estimate the fraction of selectively neutral alleles (denoted by pneut) among newly arisen mutations. it makes use of data on the distribution of rare variant alleles in large samples together with information on the average heterozygosity. the formula proposed is pneut = [he/(1-he)] [loge(2nq)/n alpha (x less than q)], where n a ...19836599962
binding specificity of serum amyloid p component for the pyruvate acetal of galactose.serum amyloid p component (sap) is a normal plasma protein that is of interest because of its presence in amyloid deposits, its presence in normal human glomerular basement membrane, and its stable evolutionary conservation. it has calcium-dependent ligand-binding specificity for amyloid fibrils, fibronectin (fn), c4-binding protein (c4bp), and agarose. although the binding to agarose, a linear galactan hydrocolloid derived from some marine algae, is unlikely per se to be related to the physiolo ...19846707579
analysis of the genome of fish lymphocystis disease virus isolated directly from epidermal tumours of pleuronectes.virions of fish lymphocystis disease virus (fldv), a member of the iridovirus family, were isolated directly from lymphocystis disease lesions of individual flatfishes and purified by sucrose and subsequent cesium chloride gradient centrifugation to homogeneity as judged by electron microscopy. the isolated fldv dnas appear to be heterogeneous in size. contour length measurements of 43 dna molecules gave an average length of 49 +/- 23 microns, corresponding to 93 +/- 44 x 10(6) d. molecular weig ...19836857994
orotic acid sodium salt in kidney stones and urinary deposits.kidney stones from a plaice, pleuronectes platessa, have been shown to consist of the sodium salt of orotic acid. precipitation of orotic acid in human kidneys and urine samples has previously been reported but the precipitates must have been salts, most likely the sodium salt, of orotic acid and not the free acid. this reinterpretation is based on the acid strength of orotic acid and on data for the solubilities of sodium orotate and orotic acid. sodium orotate is therefore a member on the list ...19836867631
calcium-dependent aggregation of human serum amyloid p component.normal human serum or isolated human amyloid p component was ultracentrifuged on density gradients containing either 10 mm edta or different concentrations of ca2+ between 0.15 and 2.15 mm. in the presence of ca2+ concentrations of 1 mm or more human p component sedimented more rapidly than it did in the presence of lower ca2+ levels or of edta. this phenomenon was due to ca2+-dependent aggregation of p component molecules and did not require the presence of any other serum constituents. it was ...19827074110
c-reactive protein and serum amyloid p component in the plaice (pleuronectes platessa l.), a marine teleost, are homologous with their human counterparts.c-reactive protein and serum amyloid p component were isolated from serum of the plaice (pleuronectes platessa l.), a murine teleost. the isolation was based on their calcium-dependent binding affinity for pneumococcal c-polysaccharide and for agarose, respectively. these specificities are the same as those of human c-reactive protein and serum amyloid p component, respectively, and we have previously reported that the plaice molecules resemble human c-reactive protein and serum amyloid p compon ...19827093286
occurrence of arsenic in plaice (pleuronectes platessa), nature of organo-arsenic compound present and its excretion by man.the arsenic content in 255 samples of plaice (pleuronectes platessa) varied between 3 and 166 mg/kg. about 65% of the samples had an arsenic content above 10 mg/kg. high (low) arsenic concentration in the fillet corresponds with a high (low) concentration in milt or roe. an excretion experiment with eight human volunteers showed that after the consumption of plaice, 69-85% of the ingested arsenic was excreted in the urine within five days. the organo-arsenic compound present in plaice was isolat ...19827140692
conservation of the tandem arrangement of alpha 1-microglobulin/bikunin mrna: cloning of a cdna from plaice (pleuronectes platessa).alpha 1-microglobulin and bikunin are both plasma proteins which are synthesized in mammalian liver from a common mrna with tandemly arranged coding sequences. here, we report a piscine homologue of mammalian alpha 1-microglobulin/bikunin mrna which was serendipitously isolated from a plaice (pleuronectes platessa) liver cdna library. the piscine cdna recognized an approximately 1300 nucleotide mrna on northern blots of plaice liver rna and, to a lesser extent, on blots of kidney and whole blood ...19947521726
the life cycle of contracaecum osculatum (rudolphi, 1802) sensu stricto (nematoda, ascaridoidea, anisakidae) in view of experimental infections.hatched, ensheathed third-stage larvae of contracaecum osculatum, 300-320 microns long, were shown to be infective to copepods, to nauplius larvae of balanus and to small specimens of fishes (sticklebacks, o-group eelpout). other fishes such as gobies and small flatfishes became infected by ingesting infected crustaceans. cod were infected by being given infected small fishes. in the crustacean paratenic hosts, little growth of the larvae occurred, if any. in the liver sinusoids of sticklebacks ...19957567906
m-band structure, m-bridge interactions and contraction speed in vertebrate cardiac muscles.cardiac muscle m-band structures in several mammals (guinea pig, rabbit, rat and cow) and also from three teleosts (plaice, carp and roach), have been studied using electron microscopy and image processing. axial structure seen in negatively stained isolated myofibrils or negatively stained cryo-sections shows the presence of five strong m-bridge lines (m6, m4, m1, m4' and m6') except in the case of the teleost m-bands in which the central m-line (m1) is absent, giving a four-line m-band. the m4 ...19947706420
unique molecular properties of superoxide dismutase from teleost fish skin.a unique cu,zn-sod was found and isolated from plaice paralichthys olivaceus skin. surprisingly, the properties of purified fish skin sod were very different from those of sod from other sources reported so far. the purified sod was composed of four same subunits of 16 kda and the molecular weight of the native sod was found to be around 65 kda. the dominant amino acids of the sod were ser, thr, pro and glu. above 70 degrees c, thermostability of the sod was much lower than that of bovine erythr ...19957875330
isolation and characterization of rainbow trout (oncorhynchus mykiss) serum amyloid p component (sap)we purified a ca(2+)-dependent agarose-binding protein from rainbow trout (oncorhynchus mykiss) sera. sds-page analysis showed the possibility that the purified protein was a polymer with a molecular weight of over 100,000 composed of covalently-bounded 32-kda subunits. n-terminal twenty amino acid sequence of the 32-kda protein showed partial homology with other known serum amyloid p components (saps) including plaice (pleuronectes platessa) (indicated 40% homology), human (55%), hamster (45%), ...19947999887
[pollution of fish and shellfish with organotin compounds and estimation of daily intake].tributyltin (tbt) and triphenyltin (tpt) compounds have been widely used as antifouling paint for ship bottoms and fishery firm nets, and they are known to be aquatic environmental contaminants. a survey of the pollution with organotin compounds in fish and shellfish has been carried out for samples (180 samples from 43 kinds) which were collected in the hokkaido prefecture during the period from february, 1989 to september, 1992. on the other hand, daily intakes of organotin compounds have been ...19948157252
cytochrome p450 1a1 cdna from plaice (pleuronectes platessa) and induction of p450 1a1 mrna in various tissues by 3-methylcholanthrene and isosafrole.a full length cdna coding for cytochrome p450 1a1 was isolated from a plaice liver cdna library constructed in lambda zapii. the deduced amino acid sequence of this cdna was 78% homologous to that of rainbow trout p450 1a1 and 57 and 51% homologous to human p450 1a1 and p450 1a2, respectively. comparisons of these sequences show the plaice cdna to be most similar to mammalian and trout p450 1a1 sequences, but also to have certain residues specific to fish p450 1a1. analysis of southern blots of ...19938193668
fish muscle structure: fibre types in flatfish and mullet fin muscles using histochemistry and antimyosin antibody labelling.in studies of the myosin crossbridge interaction with actin in vertebrate muscles, the muscles of bony fish have the unique advantage for ultrastructural work that the a-band has a simple 'crystalline' lattice of myosin filaments. however, the anatomy and physiology of these fish muscles is relatively poorly understood compared with the rabbit, chicken or frog muscles conventionally used for crossbridge studies. here the fibre types in fish fin muscles have been characterized to allow sensible s ...19938300849
influence of dietary fish proteins on plasma and liver cholesterol concentrations in rats.the effects of amount and type of dietary fish proteins on plasma and liver cholesterol concentrations were evaluated in female rats. the isonitrogenous diets used contained 10 g cholesterol/kg and were carefully balanced for residual fat, cholesterol, ca, mg and p in the protein preparations. cod meal, soya-bean protein or casein was incorporated into the diets as the only source of dietary protein at three levels: either 24, 48 or 72 g n/kg diet. extra protein was added to the diet at the expe ...19938329352
cloning and characterization of the major hepatic glutathione s-transferase from a marine teleost flatfish, the plaice (pleuronectes platessa), with structural similarities to plant, insect and mammalian theta class isoenzymes.a cdna clone (plgsta) of 896 bp, containing an open reading frame encoding a 225-amino-acid polypeptide of m(r) 25,723, was isolated from a cdna library constructed in lambda gt11 from the liver of a marine teleost flatfish, the plaice (pleuronectes platessa). the identification of this cdna as that coding for the subunit of the major cytosolic glutathione s-transferase of plaice liver, gst-a, was supported by its heterologous expression in and purification of its protein product from escherichi ...19938503846
a multi-observer study examining the radiographic visibility of fishbone foreign bodies.the use of plain radiographs to localize a suspected fishbone foreign body is the subject of controversy. accordingly radiographs of 14 species of fishbone, impacted in a soft tissue phantom, were assessed by a series of observers from the ent department (consultant surgeons, senior registrars and house officers). the agreement was assessed by graphical description of the data and tested by a spearman's rank correlation test. the overall results showed that, for the clinician, radiography is ver ...19968709081
expression of human cytochrome p4501a1 (cyp1a1) in saccharomyces cerevisiae inhibits cell division.1. saccharomyces cerevisiae cells, genetically engineered to express human cytochrome p4501a1 (cyp1a1), have a mean doubling time of 5.8 h, which is considerably slower than that of control yeast cells that have undergone the same transformation process but with a plasmid lacking cyp1a1 cdna (3.3 h). 2. a smaller reduction in the rate of cell division is observed in yeast cells expressing the closely related human p450, cyp1a2. no reduction is seen with plaice cyp1a, despite similar levels of p4 ...19968905916
purification and characterization of multiple glutathione transferase isoenzymes from grey mullet liver.fourteen isoforms of glutathione s-transferase (gst) have been separated and purified from mullet (mugil cephalus) liver by scaling up an automatic analytical method based on anionic exchange chromatography. the activity of each isoenzyme with several substrates was determined. dimeric combinations of six subunits make up this heterogeneous isoenzyme population. five of these were resolved by reverse phase chromatography; four of them, named a, b, c and d, were present in more than one isoform, ...19979368673
isolation of plaice (pleuronectes platessa) alpha1-microglobulin: conservation of structure and chromophore.a cdna coding for plaice (pleuronectes platessa) alpha1-microglobulin (leaver et al., 1994, comp. biochem. physiol. 108b, 275-281) was expressed and purified from baculovirus-infected insect cells. specific monoclonal antibodies were then prepared and used to isolate the protein from plaice liver and serum. mature 28.5 kda alpha1-microglobulin was found in both liver and serum. the protein consisted of an 184 amino acid peptide with a complex n-glycan in position asn123, one intrachain disulfide ...199910082950
the alpha1-microglobulin/bikunin gene: characterization in mouse and evolution.the 129sv mouse gene coding for the alpha1-microglobulin/bikunin precursor has been isolated and characterized. the 11kb long gene contains ten exons, including six 5'-exons coding for alpha1-microglobulin and four 3'-exons encoding bikunin. exon 7 also codes for the tribasic tetrapeptide rarr which connects the alpha1-microglobulin and bikunin parts. the sixth intron, which separates the alpha1-microglobulin and bikunin encoding parts, was compared in the human, mouse and a fish (plaice) gene. ...199910395906
toxicological characterization of sediments from baie des anglais on the st. lawrence estuary.the sediments of baie des anglais on the st. lawrence estuary have a history of environmental contamination, but no information exists relating to their toxicity. the purpose of the present study was to characterize three sites in and near baie des anglais, in terms of sediment toxicity and contaminants. sites 1 and 2 within the baie des anglais are relatively close to local industry and municipal sewage discharge points, while site 3 is outside the bay. three microscale bioassays, microtox, ech ...199910448575
indirect enzyme-linked immunosorbent assay for the identification of sole (solea solea), european plaice (pleuronectes platessa), flounder (platichthys flesus), and greenland halibut (reinhardtius hippoglossoides).polyclonal antibodies produced against soluble muscle protein extracts from sole (solea solea), european plaice (pleuronectes platessa), flounder (platichthys flesus), and greenland halibut (reinhardtius hippoglossoides) were used in an indirect enzyme-linked immunosorbent assay for the specific identification of fillets from these flatfish species. the assay was performed in two different formats: microtiter plates and immunostick tubes. immunorecognition of antibodies adsorbed to their specifi ...199910528723
vibrios associated with mortality in cultured plaice pleuronectes platessa fry.fifty two bacterial strains, identified as vibrio spp., were isolated from diseased plaice fry. the most numerous group comprised v. anguillarum (26/52), of which 3 isolates belonged to serogroup o2a, 16 corresponded to serogroup o18, and 7 isolates were non-typeable. all serogroup o18 isolates had identical ribotype patterns. fourteen isolates were identified as v. splendidus biotype i (n = 11) or v. splendidus-like (n = 3). seven isolates were v. fluvialis, representing the first isolation of ...199910605143
molecular modelling of cyp1 family enzymes cyp1a1, cyp1a2, cyp1a6 and cyp1b1 based on sequence homology with cyp102.molecular modelling of a number of cyp1 family enzymes from rat, plaice and human is described based on amino acid sequence homology with the haemoprotein domain of cyp102, a unique bacterial p450 of known structure. the interaction of various substrates and inhibitors within the putative active sites of rat cyp1a1, human cyp1a2, a fish cyp1 enzyme cyp1a6 (from plaice) and human cyp1b1, is shown to be consistent with p450-mediated oxidation in each example or, in the case of inhibitors, mechanis ...199910614688
thyroid hormone deiodination in tissues of american plaice, hippoglossoides platessoides: characterization and short-term responses to polychlorinated biphenyls (pcbs) 77 and 126.we have described the tissue distribution and properties of thyroid hormone (th) deiodination activities of the marine american plaice, hippoglossoides platessoides. we then studied the 1- or 4-week responses of the plaice liver and brain deiodination activities and the plasma thyroxine (t4) and 3,5,3'-triiodothyronine (t3) levels to an intraperitoneal injection (5-500 ng/g) of the polychlorinated biphenyl (pcb) congeners 77 (3,3'-4,4'-tetrachlorobiphenyl) or 126 (3,3',4,4',5-pentachlorobiphenyl ...200011246509
[kinetics of arsenic in human blood after a fish meal].it is well known that fish contains high amounts of arsenic (as) compounds (mean values per wet weight [mg x kg-1] ballin [1]: 41; falconer et al. [2]: 14; staveland et al. [3]: 5.2), which are mainly represented by organic as compounds, especially by arsenobetaine. it is generally assumed that arsenobetaine is rapidly eliminated via the urine and therefore seems to be non-toxic for humans. however, the kinetics of arsenobetaine in human blood are unknown to date. therefore, the following experi ...200111272865
allergens from fish and egg.allergens from fish and egg belong to some of the most frequent causes of food allergic reactions reported in the literature. egg allergens have been described in both white and yolk, and the egg white proteins ovomucoid, ovalbumin, ovotransferrin and lysozyme have been adopted in the allergen nomenclature as gal d1-d4. the most reported allergen from egg yolk seems to be alpha-livitin. in fish, the dominating allergen is the homologues of gad c1 from cod, formerly described as protein m. a clos ...200111298006
identification of cytochrome p450 1b-like sequences in two teleost fish species (scup, stenotomus chrysops and plaice, pleuronectes platessa) and in a cetacean (striped dolphin, stenella coeruleoalba).the cytochromes p450 (cyp) constitute a multigene family of enzymes playing a critical role in the oxidation of many endogenous and xenobiotic substrates. the cyp1 family is of particular interest in environmental toxicology because its members are dominant in the metabolism of polycyclic aromatic hydrocarbons (pahs), polychlorinated biphenyls (pcbs) and aryl amines. three members of the cyp1 family, cyp1a1, cyp1a2, and cyp1b1, have been identified in mammals. we report here on the identificatio ...200011460754
intercomparison studies on distribution and coassociations of heavy metals in liver, kidney, and muscle of harbor porpoise, phocoena phocoena, from southern baltic sea and coastal waters of denmark and greenland.the concentrations of selected metals such as cd, pb, cu, zn, cr, ni, mn, and fe were determined in liver, kidney, and muscle of harbor porpoise (phocoena phocoena) from three geographical regions, i.e., the baltic sea and danish and greenland coastal waters. the concentrations of cd in liver and kidney increased with age of the specimens analyzed. significant interspatial variations in both hepatic and renal levels of cd were also observed. average hepatic levels of cd in baltic, danish, and gr ...200211994793
molecular identification of nine commercial flaffish species by polymerase chain reaction-restriction fragment length polymorphism analysis of a segment of the cytochrome b region.commercial refrigerated or frozen flatfish fillets are sometimes mislabeled, and identification of these mislabeled products is necessary to prevent fraudulent substitution. identification of nine commercial flatfish species (order pleuronectiformes), hippoglossus hippoglossus (halibut), lepidorhombus boscii (four-spotted scaldfish), lepidorhombus whiffiagonis (megrin), platichthys flesus (flounder), pleuronectes platessa (european plaice), reinhardtius hippoglossoides (greenland halibut), scoph ...200212092715
developing effective environmental indicators--does a new dog need old tricks?environmental indicators are being increasingly used to emphasise the relevance of monitoring. however, indicators must also be effective. in particular, they must be able to answer management questions, be accessible to the target audience and be sufficiently precise. here, we use a time series of mercury levels in plaice from liverpool bay to illustrate how specific management questions can be formulated, answered, and the precision of these answers assessed. we also show how a programme desig ...200212398367
molecular evidence for multiple udp-glucuronosyltransferase gene familes in fish.the presence of multiple distinct ugt genes in fish was derived by analysis of dna sequence data derived the zebrafish est project, confirming indications from previous protein purification studies in another fish species, the plaice, for a diversity of isoforms in lower vertebrates. at least 10 different ugts can be identified from nucleotide sequence data in zebrafish. phylogenetic analysis of exon 1 sequences of the zebrafish, plaice and human ugts indicates that six of these genes are relate ...200212408571
evidence from heterologous expression of glutathione s-transferases a and a1 of the plaice (pleuronectes platessa) that their endogenous role is in detoxification of lipid peroxidation products.cdna clones for glutathione s-transferases a (gst-a) and a1 (gst-a1) from plaice (pleuronectes platessa) were expressed as n-terminally 6xhis tagged proteins in escherichia coli and purified to homogeneity from ni-nta silica. gst-a was an efficient catalyst for conjugation of unsaturated alkenals derived from peroxidation of polyunsaturated fatty acids with the highest activity observed with trans-non-2-enal (8 micromol min(-1) mg(-1)). gst-a1 was a very efficient se-independent glutathione pero ...200212408573
a possible approach for the assessment of radiation effects on populations of wild organisms in radionuclide-contaminated environments?it is frequently asserted that measures to protect the biotic environment from increased radiation exposures arising from human activities should be focussed on the population rather than the individual. it is, however, difficult (if not impossible) to identify any population-specific attributes that can be affected by radiation exposure directly rather than through the mediation of direct, known and identifiable effects in individual organisms. indeed, it is often conceded that this difficulty ...200312590077
organohalogens and metals in marine fish and mussels and some relationships to biological variables at reference localities in norway.the main goals of this study were to define, for the norwegian coast, "high background" levels of micropollutants routinely monitored within the the oslo and paris commission joint assessment and monitoring programme, and to reassess the present norwegian reference levels used as the basis for a classification of environmental quality. the indicator organisms analysed as bulked/individual samples were cod (gadus morhua, n approximately 1170; n approximately 240 for organochlorines), dab (limanda ...200312604072
health of flatfish from localities in placentia bay, newfoundland, contaminated with petroleum and pcbs.this study compared the health of flatfish inhabiting two areas in placentia bay, newfoundland, contaminated either with polycyclic aromatic hydrocarbons (pahs) or polychlorinated biphenyls (pcbs) with samples from reference sites. initially, four species of flatfish, including american plaice (hippoglossoides platessoides), yellowtail flounder (limanda ferruginea), gray sole (glyptocephalus cynoglossus), and winter flounder (pleuronectes americanus), were sampled only for external lesions from ...200312712279
novel antimicrobial peptides derived from flatfish genes.we report on the identification of active novel antimicrobials determined by screening both the genomic information and the mrna transcripts from a number of different flatfish for sequences encoding antimicrobial peptides, predicting the sequences of active peptides from the genetic information, producing the predicted peptides chemically, and testing them for their activities. we amplified 35 sequences from various species of flatfish using primers whose sequences are based on conserved flanki ...200312878506
[remarks on pomphorhynchus laevis (zoega, muller 1776) as a parasite in pleuronectes flesus and pleuronectes platessa]. 195713457088
intragastric formation and modulation of n-nitrosodimethylamine in a dynamic in vitro gastrointestinal model under human physiological conditions.human exposure to carcinogenic n-alkylnitrosamines can occur exogenously via food consumption or endogenously by formation of these compounds through nitrosation of amine precursors. information on the intragastric formation of ndma from complex mixtures of precursors and inhibitors in humans is not available. in this study the formation of n-nitrosodimethylamine (ndma) has been quantitatively analysed in a dynamic in vitro gastrointestinal model, in which gastric conditions can be modulated and ...200414630130
investigations on the orf 167l of lymphocystis disease virus (iridoviridae).the predicted open reading frame 167l (orf 167l) of lymphocystis disease virus (lcdv, iridoviridae ) isolated from plaice, dab and flounder was investigated. the orf 167l corresponding genes of the three lcdv isolates were amplified, cloned and sequenced. a comparison of the lcdv strains showed that the nucleotide sequence of orf 167l and its deduced amino acid sequence were highly conserved in the genus lymphocystivirus (a homology of 80% in dab and flounder/plaice, 97% in plaice and flounder). ...200414739649
pseudomonas infection in a plaice. 195014804245
ellipsomyxa gobii (myxozoa: ceratomyxidae) in the common goby pomatoschistus microps (teleostei: gobiidae) uses nereis spp. (annelida: polychaeta) as invertebrate hosts.nereis diversicolor o.f. müller and n. succinea frey et leuckart (polychaeta, nereidae) living in brackish shallow areas in denmark are naturally infected with tetractinomyxon actinospores. infected nereis spp. were experimentally fed to various potential fish hosts, and the actinosporean stages developed into myxosporean stages of ellipsomyxa gobii køie, 2003 (ceratomyxidae) in the gallbladder of the common goby pomatoschistus microps (krøyer) (gobiidae). the european eel anguilla anguilla (l.) ...200415139372
cdna cloning, sequence analysis and expression of 3-methylcholanthrene-inducible cytochrome p450 1b1 in carp (cyprinus carpio).cytochrome p450 (cyp) genes, which make up a large gene superfamily, are known to play an important role in drug metabolism. the levels of expression of cyp genes in the tissue of fish inhabiting polluted areas have been used extensively in biomonitoring studies as indicators of dioxin pollution. complementary dna of cytochrome cyp1b1 was isolated from carp (cyprinus carpio) liver 24 h after the injection of 3-methylcholanthrene (3-mc). the full length cdna obtained contained a 5' noncoding regi ...200415746899
isolation of cdna of novel cytochrome p450 1b gene, cyp1b2, from carp (cyprinus carpio) and its induced expression in gills.cytochrome p450 (cyp) enzymes constitute a multigene family of many endogenous and xenobiotic substances. the cyp1 family is of particular interest in environmental toxicology because its members are dominant in the metabolism of polycyclic aromatic hydrocarbons (pahs), polychlorinated biphenyls (pcbs), and aryl amines. a new complementary dna of the cyp1b subfamily encoding cyp1b2 was isolated from carp liver after intraperitoneal injection with 3-methylcholanthrene (3-mc). the obtained full-le ...200415750580
complementary dna cloning and constitutive expression of cytochrome p450 1c1 in the gills of carp (cyprinus carpio).cytochrome p450 (cyp) enzymes constitute a multigene family of many endogenous and xenobiotic substances. the cyp1 family is of particular interest in environmental toxicology because its members are dominant in the metabolism of polycyclic aromatic hydrocarbons (pahs), polychlorinated biphenyls (pcbs), and aryl amines. a new complementary dna of the cyp1c subfamily encoding cyp1c1 was isolated from carp liver after intraperitoneal injection of beta-napthoflavone (bnf). the full-length cdna obta ...200515915151
ontogeny of humoral immune parameters in fish.the first appearance of igm in lymphocytes varies considerably among fish species. generally, the first appearance of b-lymphocytes and immunoglobulins is late in marine species compared to fresh water species, and larvae have reached about 20-30 mm in length when igm is first expressed. rainbow trout and channel catfish show the first appearances of surface igm at about 1 week after hatching. marine species like the sea bass, spotted wolffish and cod show igm positive lymphocytes 1-10 weeks aft ...200515916905
prevalence of viral haemorrhagic septicaemia virus in danish marine fishes and its occurrence in new host species.in order to analyse the occurrence of viral haemorrhagic septicaemia virus (vhsv) in the marine waters around denmark, staff from the danish institute for food and veterinary research participated in 5 research cruises during 1998 to 2002 as a follow-up to 4 research cruises performed in 1996 to 1997. in total, 16,655 fish were examined virologically as 3569 samples. forty fish species and 3 invertebrate species were represented. vhsv was isolated from 133 samples representing 8 species: herring ...200516231640
identification of shewanella baltica as the most important h2s-producing species during iced storage of danish marine fish.shewanella putrefaciens has been considered the main spoilage bacteria of low-temperature stored marine seafood. however, psychrotropic shewanella have been reclassified during recent years, and the purpose of the present study was to determine whether any of the new shewanella species are important in fish spoilage. more than 500 h2s-producing strains were isolated from iced stored marine fish (cod, plaice, and flounder) caught in the baltic sea during winter or summer time. all strains were id ...200516269698
suitability of bifidobacteria and thymol as biopreservatives in extending the shelf life of fresh packed plaice fillets.the present study was aimed to evaluate the microbiological and sensory characteristics of biopreserved packed fresh plaice (pleuronectes platessa), during storage.200516313401
trilosporoides platessae gen. et sp. n. (myxozoa: multivalvulida) in the plaice pleuronectes platessa (teleostei: pleuronectidae) from denmark.a new myxosporean species, trilosporoides platessae gen. et sp. n. (multivalvulida), is described from the gallbladder of the plaice pleuronectes platessa l. (pleuronectidae) from denmark. the myxospore of t. platessae is conical in side view, with a 24 microm long, pointed posterior projection. in apical view, the myxospore (diameter 9.4 microm) is round, trilobed and with three spherical polar capsules arranged peripherally, equidistant and opening peripherally through protruding tips. the pol ...200516405293
juvenile plaice (pleuronectes platessa) produce camouflage by flexibly combining two separate patterns.plaice (pleuronectes platessa) is a flatfish well-known for the ability to vary its body pattern, probably for camouflage. this study investigates the repertoire of patterns used by juvenile plaice, by describing how they respond to shifts between three artificial backgrounds. two basic patterns are under active control, fine ;spots' and coarser 'blotches'. these patterns are superimposed on a fairly uniform ground. for the six plaice studied, the levels of expression of the spot and blotch patt ...200616916964
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