| cutaneous respiration in seven sea-water teleosts. | the oxygen consumption of excised skin (mo2 is.cut.) and the normal cutaneous oxygen uptake by the skin in situ from the external medium (mo2 ext.cut.) were investigated in seven species of teleosts in normoxic sea water: butterfish (pholis gunnellus l.), cod (gadus morhua l.), five-bearded rockling (ciliata mustela l), shanny (blennius pholis l.), flounder (platichthys flesus l.), sole (solea solea l.) and eel (anguilla anguilla l.). in the butterfish, cod and rockling, all the oxygen absorbed ... | 1978 | 741099 |
| environmental contaminants and biochemical responses in flatfish from the hvaler archipelago in norway. | the levels of several environmental contaminants, including selected polyaromatic hydrocarbons (pah), organochlorines (ddt/dde, hexachlorobenzene), 15 polychlorinated biphenyl (pcb) congeners, and polychlorinated dibenzofurans and dibenzo-p-dioxins, pcdf/pcdd), and heavy metals (cd, hg, pb, and as) were analyzed in muscle and liver of three different flatfish species (dab, limanda limanda; flounder, platichthys flesus; plaice, pleuronectes platessa) caught by gill netting at different sites in t ... | 1991 | 1759844 |
| levels of environmental pollutants in male and female flounder (platichthys flesus l.) and cod (gadus morhua l.) caught during the year 1988 near or in the waterway of glomma, the largest river of norway. i. polychlorinated biphenyls. | levels of polychlorinated biphenyls, pcbs, in liver samples of 111 and 121 flounder (platichthys flesus l.) and cod (gadus morhua l.), respectively, were determined gas chromatographically by quantification of 9 individual pcb congeners. the congeners were 28, 52, 101, 118, 153, 138, 180, 170, and 209 (iupac numbering system, ballschmiter and zell 1980). the fish were caught during 1988 at 5 different stations near or in the glomma estuary, in the hvaler archipelago, norway. the mean level of th ... | 1991 | 1907124 |
| the monoclonal antibody 2g8 is carbohydrate-specific and distinguishes between different forms of vertebrate cholinesterases. | the monoclonal antibody (mab) 2g8 (subclass igg2a) raised against acetylcholinesterase (ache, ec 3.1.1.7) from electric organ of torpedo nacline timilei crossreacted with ache from torpedo marmorata, electric eel (electrophorus electricus), flounder (platichthys flesus) body muscle, rat brain, bovine brain, and human brain, this suggests that the epitope to which mab 2g8 bound had been highly conserved during evolution. no crossreaction was found with ache from human and bovine erythrocytes, nor ... | 1991 | 2040291 |
| the life cycle of pygidiopsis ardeae køie, 1990 (digenea, heterophyidae). | ocellate cercariae, consistent with the description of cercaria angularis honer, 1961, developing in rediae in hydrobia ulvae (pennant) and hydrobia ventrosa (montagu) (prosobranchia, hydrobiidae) from denmark, were shown to be larvae of pygidiopsis ardeae køie, 1990 (heterophyidae, pygidiopsinae). euryhaline fishes such as the sticklebacks gasterosteus aculeatus linnaeus and pungitius pungitius (linnaeus), and the common goby pomatoschistus microps (krøyer) are second intermediate hosts in dani ... | 1990 | 2380862 |
| steroidogenic role of the caudal neurosecretory system in the flounder, platichthys flesus. | transfer of flounders from seawater (sw) to fresh water (fw) resulted in a small reduction in circulating cortisol levels and urophysial protein storage. transfer of flounders from fw to sw resulted in a larger increase in plasma cortisol and specific urophysial protein storage. over the first 4 days after transfer from fw to sw there was a positive correlation between the observed changes in urophysial urotensin i (ui) content and plasma cortisol. this apparent steroidogenic effect of ui was su ... | 1989 | 2591719 |
| organochlorine pesticides and polychlorinated biphenyls in flatfish from the southern baltic, 1983. | hexachlorobenzene (hcb), alpha-, beta-, gamma- and delta-benzenehexachloride (bhc; hch), p,p'-dde, o,p'-ddd, o,p'-ddt, p,p'-ddd and p,p'-ddt (espilon ddt) and polychlorinated biphenlys (pcbs) levels have been determined in muscle tissue of 37 turbots psetta maxima, 27 plaices pleuronectes platessa and 106 flounders platichthys flesus netted during 1983 in a different regions in the southern part of the baltic sea. the mean levels found for flatfish muscle tissue related to wet weight were: 0.7, ... | 1985 | 3934867 |
| trace metals in flatfish from the southern baltic, 1983. | the cadmium, lead, copper, zinc, iron, manganese, and nickel concentrations have been determined in muscle tissue of 35 turbot psetta maxima, 27 plaice pleuronectes platessa and 103 flounders platichthys flesus netted in 1983 in a different regions in the southern part of the baltic sea. the method of measurement was atomic-absorption spectrophotometry. the mean values obtained for turbot, plaice and flounder were 0.012, 0.009 and 0.013 for cadmium, 0.16, 0.17 and 0.12 for lead, 0.15, 0.14 and 0 ... | 1985 | 4050125 |
| [evolution of xenomas and the double role of polynuclear neutrophils in the microsporodiosis of glugea stephani (hagenmuller, 1899) in the flounder platichthys flesus (linne, 1758)]. | glugea stephani forms xenomas in the intestinal wall and in several organs of the flounder platichthys flesus in the languedoc's ponds. four characteristic stages are recognised in the development of these xenomas: reinforcement of the wall and maintenance of the host-parasite equilibrium, inflammation, granulomaformation, cicatrisation. the role of the polymorph neutrophiles is essential, first because it harbours the sporoplasm and then because it contributes to the destruction of the spores. ... | 1985 | 4083678 |
| expression of ras gene in flounder (platichthys flesus) andred mullet (mullus barbatus). | a member of the ras gene family was identified here for the first time in two marine fish, the flounder (platichthys flesus) and the red mullet (mullus barbatus). after rt-pcr the partial sequence of ras gene (exons 1 and 2) has been cloned and sequenced from normal liver. at the nucleic acid level, a very high extent of homology with the human ras genes is observed (80% to 86%) and a perfect homology is observed at the amino acid level. the high conservation of the 5' region of the ras gene sug ... | 1995 | 7488006 |
| characteristics of 'atypical', cytochrome oxidase-negative aeromonas salmonicida isolated from ulcerated flounders (platichthys flesus (l.)). | 'atypical', cytochrome oxidase-negative variants of the fish pathogen aeromonas salmonicida, isolated from ulcerated flounder (platichthys flesus), were studied using different methods. two of the strains possessed a protein that corresponded to the a-layer protein of aer. salmonicida. the strains reacted with antibodies against the a-layer and monoclonal antibodies against the o-antigen of typical aer. salmonicida. these tests confirm that the isolates from flounder should be classified as aer. ... | 1994 | 8005836 |
| in vitro metabolism of 3,3',4,4'-tetrachlorobiphenyl in relation to ethoxyresorufin-o-deethylase activity in liver microsomes of some wildlife species and rat. | a qualitative study was performed of the capacity of hepatic microsomes of several wildlife species to metabolize 3,3',4,4'-tetrachlorobiphenyl (tcb). hepatic microsomes of species environmentally exposed to polychlorinated biphenyls (pcbs): harbour porpoise (phocoena phocoena), harbour seal (phoca vitulina), common tern (sterna hirundo), and hepatic microsomes from species experimentally exposed to pcbs: eider duck (somateria mollissima), rainbow trout (salmo gairdneri), flounder (platichthys f ... | 1994 | 8039554 |
| inhibition studies on the involvement of flavoprotein reductases in menadione- and nitrofurantoin-stimulated oxyradical production by hepatic microsomes of flounder (platichthys flesus). | inhibitors of mammalian cytochrome p450 and p450 reductase were used to investigate the enzymes in flounder (platichthys flesus) hepatic microsomes involved in the stimulation of nad(p)h-dependent iron/edta-mediated 2-keto-4-methiolbutyric acid (kmba) oxidation (hydroxyl radical production) by the redox cycling compounds menadione and nitrofurantoin. inhibitors were first tested for their effects on flounder microsomal p450 and flavoprotein reductase activities. ellipticine gave type ii differen ... | 1994 | 8071949 |
| influence of diet on the bioaccumulation of pcbs. | the levels of several chlorinated biphenyls (pcbs) congeners were analysed in muscle of flounder (platichthys flesus), which were kept in semi-field condition for 1 month. the influence of resuspended sediments and two diets (worms and shrimps) on pcb accumulation in these fish was tested. highest concentrations were among pcb 138 and 153 in both abiotic (sediment and suspended particulate matter (spm)) and biotic compartments (shrimps, worms and flounder). whereas all pcb congeners vary in conc ... | 1993 | 8108709 |
| levels of environmental pollutants in flounder (platichthys flesus l.) and cod (gadus morhua l.) caught in the waterway of glomma, norway. ii. mercury and arsenic. | the present study is a survey on mercury and arsenic pollution in two sedentary fish species stationed in or close to the glomma estuary in the hvaler archipelago, norway. mercury and arsenic were determined individually in 80 flounder (platichthys flesus l.) and 100 cod (gadus morhua l.). the fish were caught at five different localities near or in the relatively polluted glomma estuary. there were significantly higher mercury and arsenic concentrations in fillets of flounder than in fillets of ... | 1993 | 8466301 |
| colonization and establishment of pomphorhynchus laevis (acanthocephala) in an isolated english river. | the successful colonization, establishment and spread of pomphorhynchus laevis in a small, isolated, devon river 128 km from the parasite's nearest focus in dorset was followed over 11 years from 1985. the parasite was first detected in anguilla anguilla and platichthys flesus in 1988: by 1995 it had attained prevalence levels of 22.6% in a. anguilla and 43.6% in p. flesus and also occurred in 100% salmo trutta, 50% cottus gobio and noemacheilus barbatulus. as judged by prevalence, abundance, pr ... | 1996 | 8991914 |
| aromatic hydrocarbon quinone-mediated reactive oxygen species production on hepatic microsomes of the flounder (platichthys flesus l.). | the nad(p)h-dependent redox cycling of a range of eight 1 ring to 5 ring aromatic hydrocarbon (ah) quinones by hepatic microsomes of flounder (platichthys flesus) was studied in terms of oxygen consumption (clark electrode) and reactive oxygen species (ros) production (detection of hydroxyl radical by iron/edta-mediated oxidation of 2-keto-4-methiolbutyric acid). stimulated oxygen consumption was detectable for only five ah-quinones (duroquinone, 1,2- and 1,4-naphthoquinones, menadione, 9,10-phe ... | 1997 | 9214713 |
| occurrence and significance of atypical aeromonas salmonicida in non-salmonid and salmonid fish species: a review. | bacterial strains of aeromonas salmonicida included in the recognized subsp. acromogenes, subsp. masoucida, and subsp. smithia in addition to the large number of strains not included in any of the described subspecies are referred to as atypical a. salmonicida. the atypical strains form a very heterogeneous group with respect to biochemical characteristics, growth conditions, and production of extracellular proteasess. consequently, the present taxonomy of the species a. salmonicida is rather am ... | 1998 | 9696626 |
| low inducibility of cyp1a activity by polychlorinated biphenyls (pcbs) in flounder (platichthys flesus): characterization of the ah receptor and the role of cyp1a inhibition. | several studies have reported a low inducibility of hepatic cytochrome p4501a (cyp1a) activity in european flounder (platichthys flesus) following exposure to mixtures of polychlorinated biphenyls (pcbs). here we report on mechanistic studies toward understanding this low cyp1a inducibility of flounder, involving molecular characterization of the ah receptor (ahr) pathway as well as inhibition of the cyp1a catalytic activity by pcb congeners. hepatic cytosolic ahr levels in flounder were determi ... | 1998 | 9710958 |
| production of a polyclonal antibody raised against recombinant flounder p53 protein. | the cdna encoding the wild type p53 protein from flounder, platichthys flesus, was expressed in escherichia coli using the gst fusion protein system. several milligrams of recombinant p53 protein were purified. this protein displayed an apparent molecular weight of 45,000 da, a value which is very similar to xenopus p53, but significantly greater than was expected based on the length of the open reading frame. immunization of rabbits against purified p53 protein allowed the production of high ti ... | 1998 | 9827050 |
| pathology and morphology of ichthyophonus hoferi in naturally infected fishes off the swedish west coast. | the pathology and morphology of ichthyophonus hoferi was studied in naturally infected atlantic herring clupea harengus, in sprat sprattus sprattus, and in flounder pleuronectes flesus from the west coast of sweden. the pathogen was found in all organs examined, with the intensity of infection varying in different organs of the different fish species. two main phases in the life of infecting parasites were identified, 'active' and 'passive', the latter being able to switch to active. the active ... | 1998 | 9828406 |
| carbonic anhydrase in the gills of seawater- and freshwater-acclimated flounders platichthys flesus: purification, characterization, and immunohistochemical localization. | flounders platichthys flesus were investigated with respect to isolation, purification, and cellular localization of carbonic anhydrase (ca) in the respiratory system. ca was purified from gills and erythrocytes and was shown to exclusively represent a soluble enzyme with an apparent molecular weight of 30 kd. inhibition constants (ki) towards acetazolamide (actz) were 8.4.10(-9) m for erythrocyte ca and 7.6.10(-9) m for gill ca, indicating a high sensitivity to sulfonamides, as exhibited by hum ... | 1999 | 9857211 |
| a homologous radioimmunoassay for the measurement of urotensin ii in the euryhaline flounder, platichthys flesus. | a sensitive and specific homologous radioimmunoassay (ria) has been developed to measure tissue and circulating levels of the fish caudal neurosecretory system neuropeptide, urotensin ii (uii), in the euryhaline flounder platichthys flesus. a polyclonal antiserum was raised against flounder uii in rabbit; uii-125i was produced by the iodogen method and purified by hplc. antiserum specificity to flounder uii was demonstrated through lack of cross-reactivity with several small peptides and paralle ... | 1999 | 10208773 |
| detection of methanogenic archaea in seawater particles and the digestive tract of a marine fish species. | a methanogen-specific nested pcr approach was used to detect methanogenic archaea in seawater particles of the north sea and the feces and the digestive tract of flounder (platichthys flesus), a fish found in the north sea. a number of 16s rdna sequences with 97.6-99.5% similarity to methanococcoides methylutens were found in the seawater particles as well as the digestive tract and fecal samples. | 1999 | 10220894 |
| occurrence of trichodinid ciliates (peritricha : urceolariidae) in the kiel fjord, baltic sea, and its possible use as a biological indicator. | investigations on the occurrence of trichodinid ciliates from fish caught in the kiel bight and kiel fjord (western baltic sea) were carried out between september 1996 and march 1997. smears of the gills, fins, and skin of 120 gadus morhua and 92 platichthys flesus caught by fish traps and trammel nets revealed the presence of trichodinid ciliates. according to the fish species and locality, different prevalences and densities of trichodinid ciliates were found. fish caught in the kiel bight rev ... | 1999 | 10431740 |
| the extent of oestrogenic contamination in the uk estuarine and marine environments--further surveys of flounder. | in 1996, the centre for environment, fisheries and aquaculture science (cefas) initiated a project to establish whether oestrogenic materials are present in uk estuarine and marine waters at biologically significant concentrations, and to investigate some of the possible effects which these may have in flounder (platichthys flesus). early results are published elsewhere; this paper describes the results of a second wider survey of vitellogenin and reproductive abnormalities in uk flounder. vitel ... | 1999 | 10492895 |
| isolation of viral haemorrhagic septicaemia virus (vhsv) from wild marine fish species in the baltic sea, kattegat, skagerrak and the north sea. | in order to analyse the occurrence of viral haemorrhagic septicaemia virus (vhsv) in the marine environment surrounding denmark, fish tissue samples were collected on four cruises with the research vessel h/s dana in 1996 and 1997. the sampling comprised 923 samples totalling 7344 fish representing 29 different species. vhsv was isolated from 24 fish samples from the baltic sea, four samples from skagerrak and three samples from the north sea. the virus-positive host species included herring clu ... | 1999 | 10509720 |
| the parasite community infecting flounders, platichthys flesus, in the tidal thames. | the composition of the parasite fauna of the flounder, platichthys flesus, retrieved from two locations in the tidal thames is described in detail for the first time. the combined parasite species list of the flounders from lots road in the upper tideway and west thurrock in the middle tideway consisted of one protozoan (glugea stephani), one monogenean (gyrodactylus sp.), four larval digeneans (cryptocotyle concava, timoniella imbutiforme, t. praeterita, and labratrema minimus), five adult dige ... | 1999 | 10526412 |
| indirect enzyme-linked immunosorbent assay for the identification of sole (solea solea), european plaice (pleuronectes platessa), flounder (platichthys flesus), and greenland halibut (reinhardtius hippoglossoides). | polyclonal antibodies produced against soluble muscle protein extracts from sole (solea solea), european plaice (pleuronectes platessa), flounder (platichthys flesus), and greenland halibut (reinhardtius hippoglossoides) were used in an indirect enzyme-linked immunosorbent assay for the specific identification of fillets from these flatfish species. the assay was performed in two different formats: microtiter plates and immunostick tubes. immunorecognition of antibodies adsorbed to their specifi ... | 1999 | 10528723 |
| the detection of biomarkers of genotoxin exposure in the european flounder (platichthys flesus) collected from the river tyne estuary. | the tyne estuary (north east england) is known to contain elevated levels of polycyclic aromatic hydrocarbons (pah), compared with other less industrialised english waterways. previous studies suggest that such contamination is responsible for the toxicity detected in invertebrate bioassays conducted on water and sediment samples collected from the tyne. here we present data from a biomonitoring study using hepatic dna adducts (32p-postlabelling assay) and bile metabolites (synchronous fluoresce ... | 1999 | 10613191 |
| cdna cloning and expression of two ki-ras genes in the flounder, platichthys flesus, and analysis of hepatic neoplasms. | the screening of a flounder cdna library with a partial sequence of ras gene from flounder (exons 1 and 2) allowed the isolation of two complete cdna sequences (ras1 and ras2) highly homologous to human ki-rasb genes. ras1 and ras2 sequences have an homology of 77.3% indicating that they represent two distinct genes, which differ particularly in their 3 regions. ras1 and ras2 intron 1 sequencing revealed an homology of only 50%, confirming that they represent two different genes. both genes enco ... | 2000 | 10825661 |
| trichodinid ciliates (peritrichia: trichodinidae) from the bay of kiel, with description of trichodina claviformis sp. n. | investigations on the epizoic fauna of gadus morhua (l.), platichthys flesus (l.) and oncorhynchus mykiss (walbaum) from the kiel fjord and kiel bight were carried out from september 1996 to march 1997. smears from 120 g. morhua and 92 p. flesus caught using fish traps and trammel nets, and of 35 o. mykiss obtained from a local fish farm in the kiel fjord revealed the presence of three species of trichodinid ciliates, trichodina claviformiis sp. n., trichodina jadranica haider, 1964 and trichodi ... | 2000 | 10945730 |
| properties of a carbonic anhydrase inhibitor protein in flounder serum. | the blood serum of the european flounder platichthys flesus strongly inhibits soluble erythrocytic carbonic anhydrase from the same species. the inhibition is of the uncompetitive type. hence, the mechanism of the carbonic anhydrase inhibition is different from that of all other known carbonic anhydrase inhibitors. the serum showed no inhibitory effect on carbonic anhydrase from human and bovine red blood cells. by applying the (18)o exchange reaction, it could be demonstrated that the presence ... | 2000 | 10976036 |
| experimental infections of copepods and sticklebacks gasterosteus aculeatus with small ensheathed and large third-stage larvae of anisakis simplex (nematoda, ascaridoidea, anisakidae). | free-swimming ensheathed larvae of anisakis simplex were shown experimentally to be ingested by the copepods oitona similis and acartia tonsa and by the nauplii of barnacles balanus sp. the larvae did not grow in the copepod hemocoel. experimental infections of various malacostracans were unsuccessful. sticklebacks gasterosteus aculeatus are naturally infected with larvae of a. simplex in coastal brackish water. such sticklebacks may have acquired the infection by eating either a crustacean host ... | 2001 | 11199846 |
| cloning and characterization of an arginine vasotocin receptor from the euryhaline flounder platichthys flesus. | a sequence coding for an arginine vasotocin (avt) receptor has been identified by the screening of a hepatic cdna library from the teleost platichthys flesus. the 2701-bp receptor sequence is predicted to yield a 384-amino acid peptide, analysis of which indicates a seven-transmembrane spanning sequence typical of g-protein-coupled receptors with the n terminus on the outer surface of the cell membrane. sequence analysis showed this sequence to have a high homology with the catostomus commersoni ... | 2001 | 11356043 |
| pathology of the liver, kidney and gonad of flounder (platichthys flesus) from a uk estuary impacted by endocrine disrupting chemicals. | the mersey estuary is highly contaminated with xenobiotics compared to the nearby dee estuary. male flounder, a migratory flatfish caught in the mersey frequently contains high blood concentrations of the oestrogen controlled, female protein vitellogenin, suggesting that mersey flounder have been exposed to endocrine disrupting contaminants. males caught from the dee contain lower blood vitellogenin levels. preliminary histopathological examination of 410 flounder from both estuaries, focuses on ... | 2000 | 11460705 |
| short-time induction of oxidative stress in hepatocytes of the european flounder (platichthys flesus). | oxidative stress induced by xenobiotic compounds has been studied using primary hepatocytes of juvenile european flounder (platichthys flesus l.) caught in a low polluted area of the german bight, tiefe rinne (landwüst et al., 1996.). cells were exposed to known oxidative stressors such as hydrogen peroxide and benzo[a]pyrene (b[a]p) in various concentrations (50 and 100 microm) up to 6 days. cell mortality was determined using fluorescent ethidium homodimer-1 and calcein am. oxidative stress re ... | 2000 | 11460738 |
| increased potential for nad(p)h-dependent reactive oxygen species production of hepatic subcellular fractions of fish species with in vivo exposure to contaminants. | the present study investigated the proposed involvement of contaminant-stimulated reactive oxygen species (ros) production in disease processes in fish. nad(p)h-dependent ros production of subcellular fractions was determined by the iron/edta-mediated oxidation of 2-keto-4-methiolbutyric acid. hepatic cytosolic nadph-dependent and microsomal nad(p)h-dependent ros production were increased 51-160% (p < 0.05) in rainbow trout (oncorhynchus mykiss) 15 weeks after a single i.p. injection of polychlo ... | 2000 | 11460751 |
| time-dependent accumulation of cyanobacterial hepatotoxins in flounders (platichthys flesus) and mussels (mytilus edulis) from the northern baltic sea. | there is only limited information about the accumulation of algal toxins in aquatic organisms in the baltic sea. in this study we measured total cyanobacterial hepatotoxin levels in blue mussel (mytilus edulis) and flounderi (platichthys flesus) tissues. flounder were caught with gillnets from the western gulf of finland during july and august 1999. blue mussels were collected from an enclosure at 3 m depth and from an artificial reef (wreck, 25-35 m depth) in the western gulf of finland between ... | 2001 | 11501282 |
| organic contaminants and trace metals in flounder liver and sediment from the amsterdam and rotterdam harbours and off the dutch coast. | organic contaminants [polychlorinated biphenyls (pcbs), organochlorine pesticides (ocps), polybrominated diphenylethers (pbdes), nonylphenols], organotin compounds and trace metals (cadmium, chromium, mercury and zinc) were determined in flounder (platichthys flesus) liver and sediment from the amsterdam harbour (north sea canal) and rotterdam harbour (euromonding) and off the dutch coast between the amsterdam and rotterdam harbour mouths in order to assess the level of contamination in these ha ... | 2001 | 11523438 |
| a comparison of freshwater and marine/estuarine strains of pomphorhynchus laevis occurring sympatrically in flounder, platichthys flesus, in the tidal thames. | collections of flounder, platichthys flesus, at two sites on the tidal river thames in 1994 and 1995 have, for the first time, revealed the sympatric occurrence of the freshwater and marine/estuarine strains of the acanthocephalan parasite pomphorhynchus laevis. this natural co-occurrence of the strains has been employed to compare infection levels and a range of parasite attributes of the two strains under conditions of sympatry. at both lots road (chelsea) and tilbury the marine/estuarine stra ... | 2001 | 11551313 |
| structure, expression and activation of fish ras genes. | ras genes encode proteins that play a central role in cell growth signaling cascades. the fish ras genes characterized to date, have a high degree of nucleotide sequence and deduced amino acid similarity with the mammalian ras gene counterparts. a large proportion and wide variety of mammalian tumors possess mutant forms of ras. in such cases, the localization of ras mutations has been restricted to exons i and ii, and to codons 12, 13 and 61. experimental exposure of fish to a range of genotoxi ... | 2001 | 11551618 |
| incorporation of organic tritium (3h) by marine organisms and sediment in the severn estuary/bristol channel (uk). | discharges of tritium (3h) into the severn estuary/bristol channel (uk) arise from the authorized release of wastes from nuclear power plants at hinkley point and berkley/oldbury and from the nycomed-amersham radiochemical plant, via the sewer system, at cardiff. the wastes from the nuclear power plants probably consist almost entirely of 3h2o, whereas those from the radiochemical plant also include uncharacterized 3h labelled organic compounds. the total 3h concentrations in demersal fish and o ... | 2001 | 11693639 |
| assessment of organotin pollution along the polish coast (baltic sea) by using mussels and fish as sentinel organisms. | levels of tributyltin (tbt) and its degradation products, mono- (mbt) and dibutyltin (dbt), as well as triphenyltin (tpt), were monitored in 10 stations along the polish coast (baltic sea). mussel-mytilus edulis-and fish-platichthys flesus-were used as sentinel organisms. the bioaccumulation patterns of butyltin and phenyltin compounds varied substantially. butyltins were detected in mussel tissue from all the sampled stations. among them, organisms from the gulf of gdansk showed the highest res ... | 2002 | 11993632 |
| molecular identification of nine commercial flaffish species by polymerase chain reaction-restriction fragment length polymorphism analysis of a segment of the cytochrome b region. | commercial refrigerated or frozen flatfish fillets are sometimes mislabeled, and identification of these mislabeled products is necessary to prevent fraudulent substitution. identification of nine commercial flatfish species (order pleuronectiformes), hippoglossus hippoglossus (halibut), lepidorhombus boscii (four-spotted scaldfish), lepidorhombus whiffiagonis (megrin), platichthys flesus (flounder), pleuronectes platessa (european plaice), reinhardtius hippoglossoides (greenland halibut), scoph ... | 2002 | 12092715 |
| parasite populations and communities from the shallow littoral of the orther bight (fehmarn, sw baltic sea). | parasites of host guilds, such as mud snails (five species), benthic crustaceans (six species) and small-sized fishes ( pomatoschistus microps, gasterosteus aculeatus, pungitius pungitius, young pleuronectes flesus), were investigated in the other bight off west fehmarn (kiel bight, german baltic sea). the hosts, especially the herbivorous hydrobia spp, gammarus spp and idothea chelipes, attained extremely high densities in three habitats ( enteromorpha belt, fucus belt, sandy bottom), which may ... | 2002 | 12122431 |
| antibacterial, antifungal and cytotoxic activities of extracts from fish epidermis and epidermal mucus. | annual discards from the world fisheries are estimated to be approximately 20 million metric tonnes (25%) per year. the main objective of this work is to increase the utilisation of by-products (notably skin) from fish species in order to isolate new biologically active compounds. this study presents the results of a screening program for antifungal, antibacterial and cytotoxic activities in epidermal mucus and epidermis extracts of thirteen fish species that are commonly caught in north atlanti ... | 2002 | 12385701 |
| disorder and recovery of environmental health monitored by means of lysosomal stability in liver of european flounder (platichthys flesus l.). | from 1995 to 2000 biological effects were studied in liver of flounder (platichtysflesus l.) from the german bight. during the study period deleterious consequences of acute discharges of ddt and pcbs in early spring 1996 and after 1998 due to remobilization of contaminants from riverbed deepening of the river elbe became evident. as core biomarker which reflects toxically induced liver pathologies and integrates effects of various classes of pollutants we measured the integrity of lysosomal mem ... | 2002 | 12408619 |
| estrogenic effects in fish in the netherlands: some preliminary results. | recently, a large-scale field study in the netherlands has focused on the effects of estrogenic contaminants on feral fish populations. the freshwater bream (abramis brama) and the estuarine flounder (platichthys flesus) were sampled at a large number of locations in the spring and autumn of 1999. concentrations of the yolk protein vitellogenin (vtg) in blood plasma of male flounders were small at most sites. at two sites, however, moderately elevated concentrations were found in autumn. both si ... | 2002 | 12505300 |
| isolation, synthesis, and biological activity of flounder [asn1,ile5,thr9] angiotensin i. | a novel angiotensin i (ang i) has been isolated from incubates of plasma and kidney extracts of the flounder, platichthys flesus, using ion-exchange, gel-permeation, and reverse-phase high performance liquid chromatography (hplc). its sequence was determined as h-asn-arg-val-tyr-ile-his-pro-phe-thr-leu-oh by sequence analysis and mass spectrometry. no vasopressor activity was detected at the elution position of [asp(1)] ang i in ion-exchange hplc. the sequence was confirmed by identity of the el ... | 2003 | 12535630 |
| bioaccumulation and detoxication of nodularin in tissues of flounder (platichthys flesus), mussels (mytilus edulis, dreissena polymorpha), and clams (macoma balthica) from the northern baltic sea. | cyanobacterial hepatotoxin accumulation in mussels (mytilus edulis, dreissena polymorpha), clam (macoma balthica), and flounder (platichthys flesus) tissues was measured. flounder were caught with gillnets from the western gulf of finland on 21 august 1999, 25 july 2000, and 25 august 2000. blue mussels were collected from: (1) a steel cage at a depth of 3 m on 20 august 1999, (2) an enclosure at depths of 3-5 m, and (3) an artificial reef (wreck at 25-30 m) in the western gulf of finland betwee ... | 2002 | 12568468 |
| determination of tissue and plasma concentrations of pthrp in fish: development and validation of a radioimmunoassay using a teleost 1-34 n-terminal peptide. | a specific and sensitive radioimmunoassay (ria) for the n-terminus of sea bream (sparus auratus) and flounder (platichthys flesus) parathyroid hormone-related protein (pthrp) was developed. a (1-34) amino-terminal sequence of flounder pthrp was synthesized commercially and used as the antigen to generate specific antiserum. the same sequence with an added tyrosine (1-35(tyr)) was used for iodination. human (1-34) parathyroid hormone (pth), human (1-34) pthrp, and rat (1-34) pthrp did not cross-r ... | 2003 | 12899855 |
| [remarks on pomphorhynchus laevis (zoega, muller 1776) as a parasite in pleuronectes flesus and pleuronectes platessa]. | | 1957 | 13457088 |
| the european flounder (platichthys flesus) tp53 functions as a temperature-sensitive transcription factor which inhibits cell growth in yeast. | numerous studies focus on biological roles of the tp53 tumor suppressor gene in mammals but little is known about the actual function of tp53 in lower vertebrates. in this study, we used an in vivo functional assay in yeast to address the transactivation capacity of the flounder tp53 protein. we showed that the flounder tp53 acts as a sequence-specific transcription factor which is able to transactivate various human promoters containing a p53-responsive element (re). this transcriptional activi ... | 2004 | 14693375 |
| low hepatic 7-ethoxyresorufin-o-deethylase (erod) activity and minor alterations in retinoid and thyroid hormone levels in flounder (platichthys flesus) exposed to the polychlorinated biphenyl (pcb) mixture, clophen a50. | the effect of the polychlorinated biphenyl (pcb) mixture clophen a50 on hepatic cytochrome p4501a1 dependent erod (7-ethoxyresorufin-o-deethylase) activity, plasma thyroid hormone levels and plasma, kidney and liver retinoid concentrations of the euryhaline flatfish flounder (platichthys flesus) was determined 2 and 10 days after i.p. (intraperitoneal) injection with 20, 100 and 500 mg clophen a50/kg body weight. no effect of clophen a50 on total cytochrome p450 content in flounder liver was obs ... | 1996 | 15091378 |
| ellipsomyxa gobii (myxozoa: ceratomyxidae) in the common goby pomatoschistus microps (teleostei: gobiidae) uses nereis spp. (annelida: polychaeta) as invertebrate hosts. | nereis diversicolor o.f. müller and n. succinea frey et leuckart (polychaeta, nereidae) living in brackish shallow areas in denmark are naturally infected with tetractinomyxon actinospores. infected nereis spp. were experimentally fed to various potential fish hosts, and the actinosporean stages developed into myxosporean stages of ellipsomyxa gobii køie, 2003 (ceratomyxidae) in the gallbladder of the common goby pomatoschistus microps (krøyer) (gobiidae). the european eel anguilla anguilla (l.) ... | 2004 | 15139372 |
| isolation of differentially expressed genes from contaminant exposed european flounder by suppressive, subtractive hybridisation. | commercially farmed european flounder (platichthys flesus) were treated with benzo[a]pyrene or cadmium under controlled laboratory conditions. in addition, animals were collected from the polluted tyne and relatively clean alde estuaries in the uk. potential differentially expressed genes were isolated from liver tissue by means of suppressive, subtractive hybridisation. a total of 284 different mrnas were identified as having the potential for being differentially expressed, of which 84 could b ... | 2004 | 15178081 |
| general condition biomarkers in relation to contaminant burden in european flounder (platichthys flesus). | in respect to the differences found for vitellogenin (vtg) expression in male and immature flounder, general biological aspects and a set of chemical residues in the liver were compared between flounder from the mersey and dee estuaries (uk). except for alpha-hch, all pollutant chemicals analysed in flounder liver differed highly significantly between mersey and dee fish. overall, the higher liver contaminant concentrations were found in vtg-induced mersey flounder. the biomarkers studied were n ... | 2004 | 15223259 |
| abnormally elevated vtg concentrations in flounder (platichthys flesus) from the mersey estuary (uk)--a continuing problem. | vitellogenin (vtg) plasma concentrations were measured in flounder (platicthys flesus l.) caught in two neighbouring uk estuaries between autumn 1997 and summer 2000. as found in previous studies, male and immature female flounder that were caught in the mersey, but not in the dee, had abnormally elevated concentrations of vtg in their plasma-evidence of the presence of natural and synthetic estrogens and/or xenoestrogens in the mersey. the present study reveals a marked seasonal cycle in vtg co ... | 2004 | 15223260 |
| hepatic ethoxyresorufin o-deethylase (erod) activity in flounder (platichthys flesus) from contaminant impacted estuaries of the united kingdom: continued monitoring 1999-2001. | the determination of hepatic ethoxyresorufin o-deethylase (erod) has been used to assess the induction of the mixed function oxygenase system (mfo) of flounder (platichthys flesus) in uk estuaries. induction of the mfo system denotes possible exposure to certain organic contaminants (e.g. polycyclic aromatic hydrocarbons, polychlorinated biphenyls) and its measure has been incorporated in national monitoring programmes. this study presents erod monitoring data from 5 uk estuaries taken between 1 ... | 2004 | 15234875 |
| analysis of micronuclei in blue mussels and fish from the baltic and north seas. | micronuclei (mn) were analyzed in erythrocytes of flounder (platichthys flesus) and wrasse (symphodus melops) and in gill cells of blue mussels (mytilus edulis). the organisms were collected from three study stations in the baltic sea and from seven stations in the north sea (karmsund area, norway) 4 times. the statistically significant differences obtained were related to the season, sex of the fish, and sampling locality. higher mn frequencies were found in fish and mussels collected from the ... | 2004 | 15269909 |
| surveys of plasma vitellogenin and intersex in male flounder (platichthys flesus) as measures of endocrine disruption by estrogenic contamination in united kingdom estuaries: temporal trends, 1996 to 2001. | plasma vitellogenin (vtg) concentrations and the presence of the ovo-testis (intersex) condition have been recorded in male flounder (platichthys flesus) captured from several united kingdom (uk) estuaries since 1996 as part of the endocrine disruption in the marine environment (edmar) project and earlier programs. it has been confirmed that plasma vtg concentrations in male flounder have remained elevated in several uk estuaries (e.g., tees, mersey, and tyne) throughout the period covered by th ... | 2004 | 15285369 |
| dna adduct analysis and histopathological biomarkers in european flounder (platichthys flesus) sampled from uk estuaries. | the presence of genotoxic and potentially carcinogenic chemical contaminants in the estuarine and coastal marine environment is well documented. in this study, european flounder (platichthys flesus) sampled from eight uk estuaries were analysed for hepatic dna adducts, using the 32p-postlabelling assay and liver histopathology as part of an on going survey to establish the health status of uk estuaries. fish were collected from the estuaries tyne, mersey, thames, alde (reference site), belfast, ... | 2004 | 15288550 |
| organochlorine contaminants in flounder (platichthys flesus) and mullet (mugil cephalus) from douro estuary, and their use as sentinel species for environmental monitoring. | in order to monitor the presence of organic pollutants in douro estuary (nw portugal), two sentinel fish species, grey mullet (mugil cephalus) and flounder (platichthys flesus), were periodically sampled from may 2001 to april 2002. at all sampling periods, several specimens of both species were allowed to depurate in clean sea water. levels of pcbs and ddts were quantified in liver and muscle of both species. the accumulation of pcbs was higher in muscle of mullet, with a maximum of 345 ng/g dw ... | 2004 | 15312718 |
| accumulation of heavy metals by flounder, platichthys flesus (linnaeus 1758), in a heterogeneously contaminated nursery area. | | 2004 | 15556199 |
| intestinal helminth parasites in flounder platichthys flesus from the river thames: an infracommunity analysis. | an analysis was undertaken of intestinal helminth communities in flounder platichthys flesus from two sites on the river thames. a comparison was made between helminth community richness and diversity from these sites at the component and infracommunity levels. at the component community level, a richer and more diverse parasite community was found in flounder from the tilbury location (marine influence) than that from the lots road location (freshwater influence). at the infracommunity level, m ... | 2004 | 15575985 |
| the gender-specific risk to liver toxicity and cancer of flounder (platichthys flesus (l.)) at the german wadden sea coast. | flatfish living in coastal areas are chronically exposed to a wide range of toxic and (pro)carcinogenic compounds derived from agriculture and industry. flounder (platichthys flesus (l.)) is the main target species for monitoring health effects of contamination in north sea and baltic sea since the species is abundant, benthic, and inhabits shallow waters such as the wadden seas and estuaries along salinity gradients into fresh water. chemical analysis in the same livers as investigated for hist ... | 2004 | 15588639 |
| physiological cost of tolerance to toxicants in the european flounder platichthys flesus, along the french atlantic coast. | physiological and genetic responses of flounder platichthys flesus populations were investigated along the french atlantic coast in one moderately contaminated estuary (ster) and three contaminated estuaries (seine, loire and gironde). the focus of this study was to explore the relationship between stress resistance and energetic trade-offs, in order to detect possible differential physiological capacities or performances between individuals carrying particular alleles or genotypes (allozyme dat ... | 2004 | 15588643 |
| electrical activity of caudal neurosecretory neurons in seawater and freshwater-adapted platichthys flesus, in vivo. | this study examined the electrical firing activity of neuroendocrine dahlgren cells in the caudal neurosecretory system (cnss) of the euryhaline flounder in vivo. intracellular recordings revealed generally similar activity patterns and membrane properties to those previously reported in vitro. to investigate the potential role of the cnss in osmoregulatory adaptation, extracellular, multiunit, recordings compared the activity patterns of dahlgren cells in fully seawater- and freshwater-adapted ... | 2005 | 15634846 |
| oxidative stress biomarkers in two resident species, mullet (mugil cephalus) and flounder (platichthys flesus), from a polluted site in river douro estuary, portugal. | exposure of marine animals to certain pollutants can enhance reactive oxygen species (ros) production with subsequent damage to macromolecules and alterations in oxidant defences levels. aimed at correlating the tissue concentration of certain contaminants (pcbs, ddt) with antioxidant defence levels and oxidative damages, two fish species with different life strategies (mullet, mugil cephalus, and flounder, platichthys flesus) were collected in the douro estuary (nw portugal). after capture, the ... | 2005 | 15642630 |
| a ranking system for the evaluation of intersex condition in european flounder (platichthys flesus). | intersex (ovotestis) is the condition whereby an individual possesses oocytes, at varying degrees of severity, within the normal testicular tissue and is regarded as an endpoint of endocrine disruption in male fish. the occurrence of intersex has been linked with exposure to endocrine-disrupting compounds present in the environment, which are thought to mimic the actions of the female sex hormone 17-beta-oestradiol. using stained histological sections of archived cases of intersex from european ... | 2004 | 15648756 |
| an integrated assessment of estrogenic contamination and biological effects in the aquatic environment of the netherlands. | an extensive study was carried out in the netherlands on the occurrence of a number of estrogenic compounds in surface water, sediment, biota, wastewater, rainwater and on the associated effects in fish. compounds investigated included natural and synthetic hormones, phthalates, alkylphenol(ethoxylate)s and bisphenol-a. the results showed that almost all selected (xeno-)estrogens were present at low concentrations in the aquatic environment. locally, they were found at higher levels. hormones an ... | 2005 | 15788174 |
| the elbe flood 2002--toxic effects of transported contaminants in flatfish and mussels of the wadden sea. | cellular changes in livers from flounder (platichthys flesus l.) and digestive glands of blue mussels (mytilus edulis) were studied to analyze whether the elbe flood catastrophe in august 2002 had any deleterious effects on animal health. flatfish and mussels were sampled at different locations in the elbe estuary and the wadden sea after the flood wave had reached the german bight. internationally used core biomarkers (lysosomal membrane stability, biotransformation enzymes) were applied to ass ... | 2004 | 15823304 |
| the "bioeffect assessment index" (bai). a concept for the quantification of effects of marine pollution by an integrated biomarker approach. | the "bioeffect assessment index" (bai) is based on the integration of several pathological endpoints measured in the liver of european flounder (platichthys flesus (l.)) during a long term study of biological effects of pollution in the german bight. the bai represents a modification of the "health assessment index" since it includes solely validated biomarkers reflecting toxically induced alterations at different levels of biological organisation in order to quantify the effects of environmenta ... | 2005 | 15907491 |
| biomarkers of contaminant exposure: results of a field study with flounder (platichthys flesus) from the southern baltic sea. | the results of the present study are based on enzyme biomarker measurements in flounder (platichthys flesus), a flatfish species that is widely distributed in baltic coastal waters. the fish were collected from known pollution gradients and from reference areas regarded as relatively free of anthropogenic input. muscular cholinesterases (ches), hepatic glutathione s-transferase (gst), and hepatic ethoxyresorufin-o-deethylase (erod) activities were measured in each sampled specimen of flounder. g ... | 2005 | 15993143 |
| stanniocalcin in the euryhaline flounder (platichthys flesus): primary structure, tissue distribution, and response to altered salinity. | stanniocalcin (stc) is a homodimeric glycoprotein hormone that was first discovered in fish, where it is largely produced by a unique endocrine gland, the corpuscles of stannius (cs). in bony fish, it is thought to be an important regulator of calcium and phosphate uptake from the aquatic environment. this report describes the molecular cloning of stc from euryhaline flounder (platichthys flesus) cs cdna and genomic dna. the flounder stc encodes a prehormone of 251 amino acids (aa) with a signal ... | 2005 | 16039656 |
| disposition of polychlorinated dibenzo-p-dioxins (pcdds) and polychlorinated dibenzofurans (pcdfs) in two norwegian epibenthic marine food webs. | polychlorinated dibenzo-p-dioxins (pcdds) and dibenzofurans (pcdfs) are a group of halogenated hydrocarbons, comprising 210 different, theoretically possible congeners. they are relatively hydrophobic and persistent to biodegradation, thereby rendering them subject to bioaccumulation. this study was conducted in frierfjord and eidangerfjord in the grenland fjord system, norway, heavily polluted by pcdd/pcdf discharges from the 16,600,859 magnesium production at herøya from 1951 to 2001. pooled s ... | 2006 | 16169571 |
| hepatic glutathione s-transferase activity in flounder collected from contaminated and reference sites along the polish coast. | the aim of the present study was to examine the activity of hepatic glutathione s-transferase (gst) in flounder, platichthys flesus, as a potential biomarker of exposure to xenobiotics present in the environment. the fish were collected along known pollution gradients and from areas regarded relatively free of anthropogenic input. gst was measured spectrophotometrically in each sampled specimen of flounder. the databases from the studies performed by two research institutes were compiled, and ge ... | 2006 | 16169590 |
| prevalence of viral haemorrhagic septicaemia virus in danish marine fishes and its occurrence in new host species. | in order to analyse the occurrence of viral haemorrhagic septicaemia virus (vhsv) in the marine waters around denmark, staff from the danish institute for food and veterinary research participated in 5 research cruises during 1998 to 2002 as a follow-up to 4 research cruises performed in 1996 to 1997. in total, 16,655 fish were examined virologically as 3569 samples. forty fish species and 3 invertebrate species were represented. vhsv was isolated from 133 samples representing 8 species: herring ... | 2005 | 16231640 |
| molecular identification of anisakis species from pleuronectiformes off the portuguese coast. | anisakid nematodes belonging to the anisakis simplex complex are highly prevalent in several fish species off the coast of portugal and are an important zoonotic problem in the iberian peninsula. two reproductively isolated sibling species of the anisakis simplex complex were identified from pleuronectiformes inhabiting the portuguese coast using restriction fragment length polymorphism (rflp). recombinant genotypes corresponding to presumptive anisakis simplex sensu stricto and anisakis pegreff ... | 2006 | 16469172 |
| molecular characterization and expression of urotensin ii and its receptor in the flounder (platichthys flesus): a hormone system supporting body fluid homeostasis in euryhaline fish. | urotensin ii (uii) is a potent vasoconstrictor in mammals, but the source of circulating uii remains unclear. investigations of the caudal neurosecretory system (cnss), considered the major source of uii in fish, alongside target tissue expression of uii receptor (ut), can provide valuable insights into this highly conserved regulatory system. we report uii gene characterization, expression of the first fish ut, and responses to salinity challenge in flounder. the 12-aa uii peptide shares 73% se ... | 2006 | 16675528 |
| measurements of biomarker levels in flounder (platichthys flesus) and blue mussel (mytilus trossulus) from the gulf of gdańsk (southern baltic). | in the framework of the eu funded beep project a set of biomarkers, gross morphometric indices and tissue concentrations of selected organic pollutants were measured in flounder (platichthys flesus) and mussels (mytilus trossulus) collected twice a year (april and october) from three sites in the inner gulf of gdańsk between autumn 2001 and spring 2003. in flounder, seasonal differences in most biomarkers were observed, but no correlations with tissue pollutant levels could be found. in mussels, ... | 2006 | 16678859 |
| influence of biological and ecological factors on the bioaccumulation of polybrominated diphenyl ethers in aquatic food webs from french estuaries. | previous studies have shown the worldwide presence of six congeners of polybrominated diphenyl ethers (pbdes) in marine biota (bde-28, -47, -99, -100, -153 and -154). the objective of the present study was to document their presence, their level and their transfer in the food web of two major estuaries in france, the loire and the seine. pbdes were quantified in eight principal species from the loire, representing primary consumers (the bivalve scrobicularia plana), omnivores (the worm nereis di ... | 2006 | 16764911 |
| biomarkers in fish from dioxin-contaminated fjords. | the grenland fjords, southern norway, have been heavily contaminated by dibenzo-p-dioxins and dibenzofurans (dioxins) over decades through inputs from a magnesium smelter. despite radically decreased inputs since 1990, there are still high levels of dioxins in both biotic and abiotic components of the fjords. the aim of the study was to establish whether biomarkers' responses in three fish species, atlantic cod (gadus morhua l.), sea-trout (anadromous brown trout, salmo trutta l.) and flounder ( ... | 2006 | 16766388 |
| differential sensitivity of flounder (platichthys flesus) in response to oestrogenic chemical exposure: an issue for design and interpretation of monitoring and research programmes. | this study was conducted as an initial investigation of 'differential response' in one of the main sentinel organisms used for monitoring programmes in united kingdom estuaries, the flounder platichthys flesus. it has been hypothesised that monitoring using species with a wide geographical spread and limited migration, such as flounder, might result in the comparison of different genetic stocks and certainly of populations with differing early life stage contaminant exposure histories. furthermo ... | 2006 | 16769108 |
| characterization and distribution of an arginine vasotocin receptor in mouse. | a cdna, which has a high homology with teleost platichthys flesus [arg8] vasotocin (avt) receptor (genbank: ak033957), was found in mouse genome database. analyses of the deduced amino acid sequence revealed that a cdna has several features of avt receptor. we tentatively named it as a mouse vasotocin receptor (mvtr). a two-electrodes voltage clamp technique was applied to characterize the mvtr expressed in xenopus laevis oocytes. avt induced ca2+-dependent cl- currents in xenopus oocytes inject ... | 2006 | 16891776 |
| analysis of nodularin-r in eider (somateria mollissima), roach (rutilus rutilus l.), and flounder (platichthys flesus l.) liver and muscle samples from the western gulf of finland, northern baltic sea. | nodularin (nodln) is a cyanobacterial hepatotoxin that may cause toxic effects at very low exposure levels. the nodln-producing cyanobacterium nodularia spumigena forms massive blooms in the northern baltic sea, especially during the summer. we analyzed liver and muscle (edible meat) samples from common eider (somateria mollissima), roach (rutilus rutilus l.), and flounder (platichthys flesus l.) for nodln-r by liquid chromatography/mass spectrometry (lc-ms) and enzyme-linked immunosorbent assay ... | 2006 | 17089704 |
| evidence for nitric oxide role in the caudal neurosecretory system of the european flounder, platichthys flesus. | a neuromodulatory role for nitric oxide has been reported for magnocellular neuroendocrine cells in mammalian hypothalamus. we examined its potential as a local intercellular messenger in the neuroendocrine dahlgren cell population of the caudal neurosecretory system (cnss) of the euryhaline flounder. immunocytochemistry using an antibody raised against human neuronal nitric oxide synthase (nos) indicated the presence of nos in the dahlgren cells. quantitative rt-pcr, using a flounder-specific p ... | 2007 | 17362951 |
| temporal and spatial variations of selected biomarker activities in flounder (platichthys flesus) collected in the baltic proper. | the aim of the study was to examine temporal (monthly) and spatial changes in the activities of a battery of biomarkers and evaluate the influence of gender and environmental factors on those activities. abiotic factors not correlated with chemical pollution-water temperature, salinity, and oxygen concentration-were evaluated. the activities of the following biomarkers-muscular ches (acetylcholinesterase (ache) and butyrylcholinesterase (bche)), hepatic ethoxyresorufin-o-deethylase (erod), gluta ... | 2008 | 17643488 |
| visual pigments of baltic sea fishes of marine and limnic origin. | absorbance spectra of rods and some cones were measured by microspectrophotometry in 22 fish species from the brackish-water of the baltic sea, and when applicable, in the same species from the atlantic ocean (3 spp.), the mediterranean sea (1 sp.), or finnish fresh-water lakes (9 spp.). the main purpose was to study whether there were differences suggesting spectral adaptation of rod vision to different photic environments during the short history (<10(4) years) of postglacial isolation of the ... | 2007 | 17822578 |
| levels of pcdd/f and dioxin-like pcb in baltic fish of different age and gender. | concentrations of polychlorinated dibenzo-p-dioxins (pcdd), polychlorinated dibenzofurans (pcdf) and polychlorinated biphenyls (pcb) in baltic sea fish like herring (clupea harengus membras), sprat (sprattus sprattus balticus), perch (perca fluviatilis), pikeperch (sander lucioperca) and flounder (platichthys flesus trachurus) collected from four areas of the estonian coastal waters are reported. all samples are studied for their relationship between the length (cm) and wet weight (g); length (c ... | 2008 | 17931686 |
| perfluoroalkyl compounds in danish wastewater treatment plants and aquatic environments. | this study reports the results of a screening survey of perfluoroalkyl compounds (pfcs) in the danish environment. the study included point sources (municipal and industrial wastewater treatment plants and landfill sites) and the marine and freshwater environments. effluent and influent water and sewage sludge were analysed for point sources. sediment, blue mussels (mytilus edulis) and liver from plaice (pleuronectes platessa), flounder (platichthys flesus) and eel (anguilla anguilla) were analy ... | 2008 | 18029290 |
| genetic analysis of infectious pancreatic necrosis virus from scotland. | infectious pancreatic necrosis (ipn) is a highly contagious disease of young salmonid fish, and is one of the most serious economic diseases in aquaculture. in scotland, an increase in ipn virus (ipnv) outbreaks in seawater atlantic salmon, salmo salar, has been reported in recent years. the aim of this study was to analyse the vp2 gene from recent ipnv isolates from scotland, to determine whether there are epidemiological links between ipnv isolates from farms (13), wild fish (17) and the envir ... | 2008 | 18086033 |
| survey of the metazoan ectoparasites of the european flounder platichthys flesus (linnaeus, 1758) along the north-central portuguese coast. | a survey was undertaken to identify metazoan ectoparasite species on the european flounder, platichthys flesus (linnaeus, 1758), in 4 different locations off the north-central portuguese coast. parasites of 7 different taxa were found: caligus diaphanus, caligus sp., and lepeophtheirus pectoralis (copepoda: caligidae); acanthochondria cornuta (copepoda: chondracanthidae); holobomolochus confusus (copepoda: bomolochidae); nerocila orbignyi (isopoda: cymotholdae); and praniza larvae (isopoda: gnat ... | 2007 | 18163362 |
| distribution of infectious pancreatic necrosis virus (ipnv) in wild marine fish from scottish waters with respect to clinically infected aquaculture sites producing atlantic salmon, salmo salar l. | this study represents the first large-scale investigation of ipnv in scottish wild marine fish. kidney samples were taken from 30 627 fish comprising 37 species and 45 isolations were made from nine different species, illustrating these as reservoirs of ipnv in scottish waters. the estimated prevalence of ipnv in the scottish marine environment was low at 0.15% (90% confidence intervals, (ci) of 0.11-0.19%). this was significantly greater in fish caught less than 5.0 km from ipn-positive fish fa ... | 2008 | 18261031 |
| histopathology and ultrastructure of platichthys flesus naturally infected with anisakis simplex s.l. larvae (nematoda: anisakidae). | the histopathology, ultrastructure, and immunohistochemistry of the alimentary canal of flounder platichthys flesus (l.), naturally infected with the nematode anisakis simplex s.l. (rudolphi 1809) from the river forth (scotland), were investigated and described. eight of the 16 flounders were infected with a. simplex s.l. larvae (l3); parasites were encapsulated by serosa on the external surface of the host's digestive tract (intensity of infection 1-8 parasites per host), although nematode larv ... | 2007 | 18314688 |
| cortisol and prolactin modulation of caudal neurosecretory system activity in the euryhaline flounder platichthys flesus. | previous studies have shown roles for cortisol and prolactin in osmoregulatory adaptation to seawater and freshwater, respectively, in euryhaline fish. this study of the european flounder investigated the potential for these hormones to modulate activity of the caudal neurosecretory system (cnss), which is thought to be involved in physiological adaptation to changing external salinity. superfusion of isolated cnss with either cortisol or prolactin (10 microm; 15 min) led to changes in firing ac ... | 2008 | 18582587 |
| accumulation and effects of nodularin from a single and repeated oral doses of cyanobacterium nodularia spumigena on flounder (platichthys flesus l.). | nodularin (nodln) is a cyclic pentapeptide hepatotoxin produced by the cyanobacterium nodularia spumigena, which occurs regularly in the baltic sea during the summer season. in this study flounder (platichthys flesus l.) was orally exposed to nodln either as a single dose or as three repeated doses 3 days apart. liver and bile samples of the fish were taken 4 days after the last dose. liver glutathione-s-transferase (gst) activity was also measured and the histopathology of the liver was investi ... | 2009 | 19002737 |
| long-term trends in the prevalence of cancer and other major diseases among flatfish in the southeastern north sea as indicators of changing ecosystem health. | this paper analyses and discusses spatial and temporal patterns in the prevalence of major skin diseases (lymphocystis, epidermal hyperplasia/papilloma, ulcers), intestinal parasite glugea sp., and liver cancer in dab (limanda limanda) and flounder (platichthys flesus) in the dutch section of the north sea since the mid-1980s. we have attempted to relate disease prevalence trends in both species to chemical contaminant exposure and other relevant environmental factors including fish condition fa ... | 2009 | 19368228 |
| two new antarctic gyrodactylus species (monogenoidea): description and phylogenetic characterization. | two new species of gyrodactylus nordmann, 1832 (gyrodactylidae, monogenoidea) are described, i.e., gyrodactylus coriicepsi n. sp. from gills of black rockcod, notothenia coriiceps richardson, 1844, and gyrodactylus nudifronsi n. sp. from gills of gaudy notothen, lepidonotothen nudifrons lönnberg, 1905, collected in waters surrounding the south shetland islands, antarctica. gyrodactylus coriicepsi n. sp. is characterized by a long, narrowing membrane of the ventral bar and a marginal hook sickle ... | 2009 | 19413367 |