| bacterial infection which produces high mortality in cultured japanese flounder (paralichthys olivaceus) in hokkaido. | | 1987 | 3430905 |
| selective agars for the isolation of streptococcus iniae from japanese flounder, paralichthys olivaceus, and its cultural environment. | two kinds of selective agar were developed for the isolation of streptococcus iniae, the causal agent of streptococcosis, from japanese flounder (paralichthys olivaceus) and from culture tanks in flounder farms. the selective agars were heart infusion agar with added thallium acetate and oxlinic acid (taoa), and colistin sulphate and oxolinic acid (csoa). for samples containing various bacterial flora, selective agars were supplemented with defibrinated horse blood in order to distinguish beta-h ... | 1999 | 10347871 |
| cloning of a calcitonin gene-related peptide receptor and a novel calcitonin receptor-like receptor from the gill of flounder, paralichthys olivaceus. | for the first time in non-mammalian vertebrates, cdna encoding cgrpr was isolated from the gill cdna library of flounder. the nucleotide sequence consists of a 237bp 5'-utr, a 1398bp coding sequence for a 465-amino-acid protein, and a 981bp 3'-utr. the predicted amino-acid sequence has a high degree of identity to hcgrpr (72.3%) and rcgrpr (71.6%) and, to a lesser degree, to hctr (55.6%) and rctr (59.3%). in addition, a different type of receptor cdna was also obtained from the gill cdna. the nu ... | 2000 | 10689190 |
| molecular cloning, characterization, and expression of tnf cdna and gene from japanese flounder paralychthys olivaceus. | we cloned a cdna and the gene for japanese flounder tnf. the tnf cdna consisted of 1217 bp, which encoded 225 amino acid residues. the identities between japanese flounder tnf and members of the mammalian tnf family were approximately 20-30%. the positions of cysteine residues that are important for disulfide bonds were conserved with respect to those in mammalian tnf-alpha. the japanese flounder tnf gene has a length of approximately 2 kbp and consists of four exons and three introns. the posit ... | 2000 | 11035080 |
| a survey of expressed genes in the leukocytes of japanese flounder, paralichthys olivaceus, infected with hirame rhabdovirus. | we constructed a cdna library of japanese flounder, paralichthys olivaceus, leukocytes that were infected with hirame rhabdovirus (hrv) in order to analyze some of the genes that are induced and expressed by virus infection in the immune system. four hundred and fifty-two partial sequences representing 300 cdna clones were obtained from the 5' and/or 3' ends of inserts derived from the japanese flounder leukocyte cdna library. about three-quarters of the 300 cdna clones (217 clones, 72.3%) repre ... | 2000 | 10689095 |
| molecular analysis of complement component c8beta and c9 cdnas of japanese flounder, paralichthys olivaceus. | the amino acid sequences of the human terminal complement components show extensive structural similarity to each other. in this study the c8beta and c9 cdnas of japanese flounder, paralichthys olivaceus, were cloned and analyzed. the derived deduced amino acid sequences of the two terminal components were homologous to those of humans, in that the sequences of both species contained ldl receptor, egf precursor, and two thrombospondin domains. japanese flounder c9 was found to have a second thro ... | 1999 | 10541805 |
| sequences of 596 cdna clones (565,977 bp) of japanese flounder (paralichthys olivaceus) leukocytes infected with hirame rhabdovirus. | : we have partially sequenced 785 sequences of 596 independent complementary dna clones isolated from a cdna library of japanese flounder leukocytes infected with hirame rhabdovirus. these sequences consist of a total of 565,977 base pairs. the average size of the sequenced lengths was 721 bp. of 596 clones, 386 (64.8%) were identified as previously reported genes by the blastn and blastx programs. about 30% of the identified clones could be recognized by only the blastx program. a total of 251 ... | 1999 | 10525682 |
| gene and cdna structures of flounder insulin-like growth factor-i (igf-i): multiple mrna species encode a single short mature igf-i. | to understand the comprehensive mechanisms of gene expression and processing for insulin-like growth factor-i (igf-i) in vertebrates, we have investigated the gene organization, promoter and transcriptional initiation sites, alternative splicing and polyadenylating sites, and the cdna structures of this gene in the japanese flounder, paralichthys olivaceus. the flounder igf-i gene was found to be composed of five exons and four introns spanning 17.5 kb. by northern blot analysis, two major mrna ... | 1998 | 9809747 |
| hemagglutinating activity of extracellular alkaline metalloendopeptidases from vibrio sp. nuf-bpp1. | alkaline metalloendopeptidase (metalloprotease) ap1 (48 kda) from vibrio sp. isolated from the intestine of a five-barred goatfish (parupeneus trifasciatus) was reported in our previous paper to produce ap2 (36 kda) by releasing a peptide fragment (molecular mass of about 12 kda) from the c-terminal end of ap1 by autodigestion. ap1 strongly agglutinated fish (flounder, paralichthys olivaceus) and rabbit erythrocytes, and weakly chicken erythrocytes. in contrast, ap2 had no significant hemaggluti ... | 1998 | 9692199 |
| molecular cloning of a novel interferon regulatory factor in japanese flounder, paralichthys olivaceus. | a complementary dna library was constructed in lambda zap ii using messenger rna from the leukocytes of some heterocloned japanese flounder, paralichthys olivaceus, that had been artificially infected with hirame rhabdovirus (hrv). a cloned flounder interferon regulatory factor (designated firf) cdna was found to be 1746 bp in length, with an open reading frame of 297 amino acids. the overall amino acid sequence of firf had approximately 40% identity with the previously reported avian and mammal ... | 1998 | 9628009 |
| a survey of expressed genes in japanese flounder (paralichthys olivaceus) liver and spleen. | expressed sequence tags (ests) were obtained from japanese flounder (paralichthys olivaceus). we present the results of single-pass sequencing of 493 ests from 350 clones from liver cdna and 57 ests from 41 clones from spleen cdna. sequences of the cdna clones were compared with sequences in the genbank database. two hundred and two clones (51.7%) appeared to be completely unknown and are likely to represent newly described genes, whereas 189 clones (48.3%) were identified based on matches to se ... | 1997 | 9418294 |
| characterization and expression of c-type lysozyme cdna from japanese flounder (paralichthys olivaceus). | lysozyme is a widely distributed enzyme located in the serum, skin mucus, and other organs of fish, which is responsible for catalyzing the hydrolysis of the cell walls of most bacteria. a c-type of lysozyme cdna was cloned from a kidney cdna library of the japanese flounder (paralichthys olivaceus). the cdnas consisted of 612 bp, which coded for 143 amino acid residues. the deduced amino acid sequence of japanese flounder c-type lysozyme possessed 72.9%, 57.4%, and 65.4% identities with rainbow ... | 1997 | 9418289 |
| construction of thiostrepton-inducible, high-copy-number expression vectors for use in streptomyces spp. | a high-copy-number plasmid expression vector (pij6021) was constructed that contains a thiostrepton-inducible promoter, ptipa, from streptomyces lividans 66. the promoter and ribosome-binding site of tipa lie immediately upstream from a multiple cloning site (mcs) which begins with a ndei site (5'-catatg) that includes the tipa translational start codon (atg), allowing the synthesis of native proteins. transcriptional terminators occur just upstream from ptipa and immediately downstream from the ... | 1995 | 8529877 |
| the antimetamorphic effect of prolactin in the japanese flounder. | prolactin has an antimetamorphic action in amphibian tadpoles. in the japanese flounder, paralichthys olivaceus, ovine prolactin (oprl, 100 ng/ml) antagonized the stimulatory effect of triiodothyronine (t3, 1 ng/ml) on the resorption of the dorsal fin rays of prometamorphic larvae in vitro. ovine growth hormone (ogh) at the same dose was without effect. injections with oprl (50 ng/fish, six times) into prometamorphic larvae also delayed the resorption of the dorsal fin rays without affecting the ... | 1994 | 8138118 |
| unique molecular properties of superoxide dismutase from teleost fish skin. | a unique cu,zn-sod was found and isolated from plaice paralichthys olivaceus skin. surprisingly, the properties of purified fish skin sod were very different from those of sod from other sources reported so far. the purified sod was composed of four same subunits of 16 kda and the molecular weight of the native sod was found to be around 65 kda. the dominant amino acids of the sod were ser, thr, pro and glu. above 70 degrees c, thermostability of the sod was much lower than that of bovine erythr ... | 1995 | 7875330 |
| characterization of ostrich (struthio camelus) beta-microseminoprotein (msp): identification of homologous sequences in est databases and analysis of their evolution during speciation. | beta-microseminoprotein, alternatively called prostatic secretory protein of 94 amino acids, is a hydrophilic, unglycosylated, small protein rich in conserved half-cystine residues. originally found in human seminal plasma and prostatic fluids, its presence was later shown in numerous secretions and its homologs were described in many vertebrate species. these studies showed that this protein had rapidly evolved, but they failed to unambiguously identify its biological role. here, we show that a ... | 2001 | 11604528 |
| effects of various doses of thyroxine and triiodothyronine on the metamorphosis of flounder (paralichthys olivaceus). | two experiments were carried out to clarify the effects of thyroxine (t4) and triiodothyronine (t3) on flounder metamorphosis. in the first experiment, three doses of t4 were administered to premetamorphic flounder larvae together with thiourea (tu). thiourea treatment (30 ppm in ambient seawater) alone caused metamorphic stasis in the fish, although the beginning of the right eye migration was not completely inhibited. t4 at 100 and 10 ppb in ambient seawater induced metamorphosis in the thiour ... | 1987 | 3666411 |
| thyroxine surge in metamorphosing flounder larvae. | the tissue concentration of thyroxine (t4) in larval flounder (paralichthys olivaceus) was studied at various stages of their metamorphosis using a specific radioimmunoassay developed for samples obtained by quantitative extraction of the hormone from fish eggs and larvae. t4 concentrations were below the limit of detection in extracts of fertilized eggs and larvae until the end of prometamorphosis. several dorsal fin rays continued growing from premetamorphosis until the end of prometamorphosis ... | 1988 | 3131186 |
| cortisol enhances the stimulating action of thyroid hormones on dorsal fin-ray resorption of flounder larvae in vitro. | effects of thyroid hormones and cortisol on fin-ray resorption of the larval flounder were studied in vitro. dorsal fin rays were dissected from the prometamorphic larvae of the japanese flounder (paralichthys olivaceus) and were cultured for 7-8 days in a medium supplemented with different concentrations of thyroxine (t4) or triiodothyronine (t3) or combinations of either thyroid hormone or cortisol (0.1 micrograms/ml). both t4 and t3 induced shortening of the second fin ray. t3 was more potent ... | 1990 | 2391025 |
| the survivability of the ectoparasitic flagellate ichthyobodo necator on chum salmon fry (oncorhynchus keta) in seawater and comparison to ichthyobodo sp. on japanese flounder (paralichthys olivaceus). | experimental studies revealed that a freshwater ectoparasitic flagellate ichthyobodo necator (henneguy, 1883) could survive and reproduce in seawater after infected chum salmon fry, oncorhynchus keta (walbaum), were transferred directly from fresh water to 33% seawater. minor morphological changes (slight reduction in body width, loss of twistlike wrinkles on body surface, and reduction in contractile vacuoles) were observed in the attached form of i. necator following transfer to seawater. the ... | 1990 | 2299525 |
| changes in cortisol and thyroid hormone concentrations during early development and metamorphosis in the japanese flounder, paralichthys olivaceus. | both cortisol and thyroid hormones were detected in newly fertilized eggs of the japanese flounder, paralichthys olivaceus. mean cortisol levels ranged around 2.5 ng/g wet weight. cortisol concentrations declined to about one-tenth of their initial levels by 2 days before hatching. the concentrations of triiodothyronine (t3; 7 ng/g) were greater than those of thyroxine (t4; 0.4 ng/g). the t3 levels decreased gradually in the eggs until the time of hatching and then decreased rapidly to undetecta ... | 1991 | 1879653 |
| assessment of dna vaccine potential for juvenile japanese flounder paralichthys olivaceus, through the introduction of reporter genes by particle bombardment and histopathology. | genetic immunisation potential, following dna bombardment for juvenile japanese flounder, paralichthys olivaceus was examined. gfp plasmids bombarded at two pressures, 150 and 300psi were sampled at 1, 7, 14 and 28 days, greater immunofluorescence was observed at the higher bombardment pressure. histopathology, at 3 h post bombardment showed considerable damage to fish epithelial and dermal tissues when bombarded at pressures greater than 200 psi, with many dna-coated gold particles present. at ... | 2000 | 11115702 |
| infection with edwardsiella tarda causes hypertrophy of liver cells in the japanese flounder paralichthys olivaceus. | to study the direct cause of liver enlargement in the japanese flounder paralichthys olivaceus infected with edwardsiella tarda, the fish were challenged with e. tarda and reared without feeding. the liver of fish exposed to the bacteria was markedly enlarged compared to that of the controls while no severe histopathological change appeared in the organ during the experiments. no notable difference was observed in the crude fat, glycogen, and water content of the liver between challenged and con ... | 2000 | 11104075 |
| identification of viral induced genes in ig+ leucocytes of japanese flounder paralichthys olivaceus, by differential hybridisation with subtracted and un-subtracted cdna probes. | up-regulated genes of leucocytes expressing immunoglobulin (ig+ leucocytes) of hirame rhabdovirus (hrv)-infected japanese flounder were identified by differential hybridisation, using subtracted and un-subtracted cdna probes. ig+ leucocytes were separated from apparently healthy and hrv-infected japanese flounder by the magnetic beads antibody method using mouse anti-japanese flounder ig monoclonal antibody (mab). a cdna library was constructed from hrv-infected japanese flounder leucocytes, and ... | 2000 | 11081439 |
| complete nucleotide sequence of japanese flounder (paralichthys olivaceus) mitochondrial genome: structural properties and cue for resolving teleostean relationships. | we cloned and sequenced the complete mitochondrial genome of japanese flounder (paralichthys olivaceus). a circular 17,090 bp mitochondrial genome from the flounder contains 37 structural genes as in other vertebrates so far reported. this is the first report of the complete mitochondrial sequence from a higher teleostean fish (acanthopterygii). the organization including gene order is quite similar to that of other teleostean fishes as well as placental mammals. the putative control region of t ... | 2000 | 10912672 |
| molecular cloning and novel repeated sequences of a c-type lysozyme gene in japanese flounder (paralichthys olivaceus). | a clone of a dna fragment of approximately 10 kb that included a c-type lysozyme gene of japanese flounder (paralichthys olivaceus) was isolated from a japanese flounder genomic dna library. this clone was subcloned, and the nucleotide sequence of the c-type lysozyme gene was determined. the flounder c-type lysozyme gene, which consisted of 3,617 bp, was found to be composed of four exons and three introns. the transcription start site was determined to be 44 bp upstream of the atg codon by prim ... | 2000 | 10852802 |
| attachment-inducing capacities of fish tissue extracts on oncomiracidia of neobenedenia girellae (monogenea, capsalidae). | when oncomiracidia of neobenedenia girellae (monogenea, capsalidae) were incubated in wells with lyophilized extracts of fish skin epithelia on the bottom, some attached to the well bottom with the haptor unfolded and shed the ciliated epidermal cells. based on these morphological changes in oncomiracidia, we developed a new assay method to examine the attachment-inducing capacities of various fish extracts for oncomiracidia. attachment-inducing capacities were found only in extracts of fish ski ... | 2000 | 10780535 |
| aromatase inhibitor and 17alpha-methyltestosterone cause sex-reversal from genetical females to phenotypic males and suppression of p450 aromatase gene expression in japanese flounder (paralichthys olivaceus). | the sex of japanese flounder (paralichthys olivaceus) is easily altered by water temperature or sex steroid hormone treatment during the period of sex determination. we have previously shown that rearing the genetically female larvae at high water temperature caused the suppression of p450 aromatase (p450arom) gene expression in the gonad and phenotypic sex-reversal of the individuals to males (kitano et al. 1999. j mol endocrinol 23:167-176). in the present study, we show that treatment of gene ... | 2000 | 10737961 |
| cloning and analysis of expression of mx cdna in japanese flounder, paralichthys olivaceus. | myxovirus resistance (mx) is an interferon (ifn)-induced protein that specifically prevents growth, in vivo and in vitro, of certain classes of virus. we isolated the mx cdna from a leukocyte cdna library obtained from a japanese flounder infected with hirame rhabdovirus (hrv). the mx cdna was found to contain 2385 bp coding for 620 amino acids. the amino acid sequence had approximately 51 and 78% identities to the sequences of human and rainbow trout mx cdnas, respectively. we propose that the ... | 2000 | 10736524 |
| identification of cdnas encoding two subtypes of vitamin d receptor in flounder, paralichthys olivaceus. | cdnas encoding two subtypes of the vitamin d receptor (vdr) are cloned from a teleost (flounder, paralichthys olivaceus). this is the first report of vdr subtypes in fish. flounder vdr (fvdr) a and b share 86% identity at the amino acid level. with human (h), rat, mouse, quail, and xenopus vdrs, fvdra shares 72%, 71%, 71%, 69%, and 71% identity, and fvdrb shares 70%, 69%, 69%, 67%, and 68% identity, respectively. the peptide sequences of the dna-binding domain (dbd) and hormone-binding domain (h ... | 2000 | 10733902 |
| a new strain of cryptocaryon irritans from the cultured olive flounder paralichthys olivaceus. | an obligate parasite, cryptocaryon irritans, which is responsible for the white spot disease of marine fish is known to develop in the temperature regime over 19 degrees c. recently, however, we found white spot disease of olive flounder paralichthys olivaceus during winter at water temperatures ranging between 12 and 16 degrees c in korea. in the present study we isolated a c. irritans-like ciliate from the affected fish and investigated its reproductive characters to compare the newly found ci ... | 2000 | 11206736 |
| purification, n-terminal amino acid sequence, and some properties of cu, zn-superoxide dismutase from japanese flounder (paralichthys olivaceus) hepato-pancreas. | cu, zn-superoxide dismutase (sod) has been purified to homogeneity from japanese flounder paralichthys olivaceus hepato-pancreas. the purification of the enzyme was carried out by an ethanol/chloroform treatment and acetone precipitation, and then followed by column chromatographies on q-sepharose, s-sepharose and ultrogel aca 54. on sds-page, the purified enzyme gave a single protein band with molecular mass of 17.8 kda under reducing conditions, and showed approximately equal proportions of 17 ... | 2001 | 11290457 |
| cloning of japanese flounder paralichthys olivaceus cd3 cdna and gene, and analysis of its expression. | two distinct cd3 homologue cdnas, cd3-1 and cd3-2, were isolated from a japanese flounder leukocyte cdna library. cd3-1 consisted of 961 bp encoding 178 amino acid residues, and cd3-2 consisted of 927 bp encoding 182 amino acid residues. the two deduced amino acid sequences had an identity of 95.1%, and neither had n-linked glycosylation sites. the identities between the japanese flounder cd3s and previously reported cd3s (cd3 epsilon, cd3 gamma, or cd3 delta) of xenopus laevis, chicken, and var ... | 2001 | 11345589 |
| cdna for an immune response gene encoding low molecular weight polypeptide from flounder, paralichthys olivaceus. | the cdna for an immune response gene encoding the low molecular weight polypeptide (lmp7) was cloned and sequenced from a flounder (paralichthys olivaceus) leukocyte cdna library. the cdna clone was 1,160 bp, and composed of an open reading frame of 822 bp that corresponded to a protein of 273 amino acid residues with a calculated mass of 30.5 kda. the scanprosite search indicated that the deduced amino acid sequence from the flounder lmp7 contains a proteasome beta-type subunit signature, which ... | 2001 | 11355705 |
| expression of japanese flounder c-type lysozyme cdna in insect cells. | lysozyme is a widely distributed hydrolase which likely plays an important role in bio-defense systems. in the current study, we constructed a baculovirus expression system for the c-type lysozyme cdna of japanese flounder (paralichthys olivaceus) and evaluated the activity of the recombinant protein. this activity was estimated, by turbidimetric assay, to be 7.7u/mg, a value five times higher than that of hen egg white (hew) c-type lysozyme examined under the same conditions. the optimum ph and ... | 2001 | 11356223 |
| high permissivity of the fish cell line ssn-1 for piscine nodaviruses. | seventeen isolates of piscine nodavirus from larvae or juveniles of 13 marine fish species affected with viral nervous necrosis (vnn) were examined for their infectivity to a fish cell line ssn-1. based on cytopathic effects (cpe) and virus antigen detection by fluorescent antibody technique (fat) after incubation at 25 degrees c, the infectivity of these virus isolates was divided into 4 groups. group 1, including 9 virus isolates from 4 species of grouper, 2 species of sea bass, barramundi, ro ... | 1999 | 11407403 |
| molecular cloning, expression and evolution of the japanese flounder goose-type lysozyme gene, and the lytic activity of its recombinant protein. | in this study, we cloned the goose-type (g-type) lysozyme gene from the japanese flounder genomic dna library, the first such data in fish and only the second after the chicken g-type lysozyme gene. the japanese flounder g-type lysozyme gene was 1252 bp in length from the transcription site to the polyadenylation site, coded for 758 bp of mrna and 195 deduced amino acids, which contain five exons and four introns. a phylogenetic analysis based on amino acid sequences showed that the flounder gen ... | 2001 | 11470157 |
| cloning of a calcitonin gene-related peptide from genomic dna and its mrna expression in flounder, paralichthys olivaceus. | a part of genomic dna including the calcitonin gene-related peptide (cgrp) gene was cloned from flounder by the genome-walking method. the intron/exon boundary was predicted to occur exactly at the same position as in salmon. the 37-amino acid molecule coded by the region from the intron/exon boundary to the stop codon was preceded by a typical lys-arg cleavage signal and included a cleavage/amidation site common to the cgrp of other vertebrates. the predicted amino acid sequence of flounder cgr ... | 2001 | 11514025 |
| ontogeny of cholecystokinin-immunoreactive cells in the digestive tract of atlantic halibut, hippoglossus hippoglossus, larvae. | the appearance and distribution of cholecystokinin (cck)-producing cells were investigated in the digestive tract of developing larvae of cultured atlantic halibut, hippoglossus hippoglossus. the cck-producing cells were detected immunohistochemically, by use of a primary antiserum against cck cloned for the japanese flounder, paralichthys olivaceus. no cck-immunoreactive (ir) cells were detected in first-feeding larvae (33 days after hatching, dah). forty-five dah or 12 days after first feeding ... | 2001 | 11551115 |
| spread of neoheterobothrium hirame (monogenea), a serious pest of olive flounder paralichthys olivaceus, to korea. | neoheterobothrium hirame is a large, blood-feeding gill-worm infecting the highly prized olive flounder paralichthys olivaceus in japan. there is strong evidence that this worm is the primary cause of anaemia, a common and serious condition causing losses among both wild and cultured olive flounders. n. hirame was first detected in japanese waters less than a decade ago, and its population then proliferated and spread throughout most of japan, except hokkaido. in neighbouring korea, olive flound ... | 2001 | 11558730 |
| cdna cloning and phylogenetic analysis of pancreatic serine proteases from japanese flounder, paralichthys olivaceus. | to better understand the digestive physiology and phylogeny of the pancreatic serine proteases of teleosts, we cloned trypsin, chymotrypsin and elastase from flounder (paralichthys olivaceus). fifty phage plaques randomly chosen from a flounder pancreatic cdna library were found to contain three species of trypsin, two species of chymotrypsin and four species of elastase. cdnas of two species of carboxypeptidase a, one carboxypeptidase b and lipase were also obtained. in total, 23 out of 24 dige ... | 2002 | 11742759 |
| an outbreak of vhsv (viral hemorrhagic septicemia virus) infection in farmed japanese flounder paralichthys olivaceus in japan. | a rhabdoviral disease occurred in farmed populations of market sized japanese flounder (hirame) paralichthys olivaceus in the seto inland sea of japan in 1996. the causative agent was identified as viral hemorrhagic septicemia virus (vhsv) based on morphological, immunological, and genetic analyses. diseased fish that were artificially injected with a representative virus isolate showed the same pathological signs and high mortality as observed in the natural outbreak. this is the first report o ... | 2001 | 11775799 |
| morphology and biology of parasite responsible for scuticociliatosis of cultured olive flounder paralichthys olivaceus. | based on the arrangement and shape of the buccal structure, scuticociliates isolated from cultured olive flounder paralichthys olivaceus belonged to the family uronematidae and showed many characteristics of uronema marinum. there was variation in the morphometry of clinical isolates taken from different organs of infected flounder. however, the isolates did not show any significant difference in morphometry under cultured conditions. the ciliates were easily maintained in in vitro medium to whi ... | 2001 | 11797915 |
| sensitive and rapid detection of edwardsiellosis in fish by a loop-mediated isothermal amplification method. | here we report a rapid and sensitive method (using loop-mediated isothermal amplification [lamp]) for the diagnosis of edwardsiellosis, a fish disease caused by edwardsiella tarda, in japanese flounder. a set of four primers was designed, and conditions for the detection were optimized for the detection of e. tarda in 45 min at 65 degrees c. no amplification of the target hemolysin gene was detected in other related bacteria. when the lamp primers were used, detection of edwardsiellosis in infec ... | 2004 | 14711699 |
| impacts of diclidophorid monogenean infections on fisheries in japan. | many monogeneans are pathogenic to economically important fish in japan. however no other monogenean is comparable with the diclidophorids, heterobothrium okamotoi and neoheterobothrium hirame, on the scale of impacts they inflict on japanese fisheries. the shared importance of the two monogenean infections lies in their pathogenicity, fecundity and tolerance to chemical treatment. heterobothrium okamotoi infects the gills and wall of the branchial cavity of the tiger puffer, takifugu rubripes ( ... | 2002 | 11835977 |
| attachment-inducing capacities of fish skin epithelial extracts on oncomiracidia of benedenia seriolae (monogenea: capsalidae). | attachment-inducing capacities of skin epithelial extracts of yellowtail, japanese flounder and red sea bream on oncomiracidia of the monogenean benedenia seriolae were examined. clear differences were not detected in the capacity among the fish species, although b. seriolae infects only yellowtail and its congeners in seriola. this suggests that either the capacity is not host specific or host-specific attachment-inducing capacity cannot be detected by the assay method. further, the attachment- ... | 2002 | 11835978 |
| genetic relatedness among japanese, american and european isolates of viral hemorrhagic septicemia virus (vhsv) based on partial g and p genes. | molecular virological analyses of 8 japanese vhsv (viral hemorrhagic septicemia virus) isolates from wild and farmed japanese flounder paralichthys olivaceus were performed to investigate their genetic relatedness to american and european isolates of vhsv. phylogenetic analyses based on the partial nucleotide sequences of g and p genes revealed that there are 2 genogroups of vhsv in japan. the first one represented by the obama25 isolate is closely related to the american isolates (genogroup i) ... | 2002 | 12005236 |
| molecular cloning and expression analysis of tumor necrosis factor alpha from a marine fish reveal its constitutive expression and ubiquitous nature. | the tumor necrosis factor alpha ( tnf alpha) gene from the marine fish, gilthead seabream (sparus aurata l.), has been isolated by rt-pcr using degenerate primers designed against vertebrate tnf alpha conserved motifs and subsequent rapid amplification of cdna ends (race). the tnf alpha cdna consists of a 142 bp 5' untranslated region (5'utr), a single open reading frame of 762 bp, which could code for a 253 amino acid protein, and a 476-bp 3'utr. the protein sequence deduced from seabream tnf a ... | 2002 | 12073149 |
| atlantic halibut growth hormone: structure and plasma levels of sexually mature males and females during photoperiod-regulated annual cycles. | the main objectives of this study were to obtain the amino acid sequence of atlantic halibut (hippoglossus hippoglossus) growth hormone (hhgh) and compare it with other teleost species, to establish a radioimmunoassay to assess plasma hhgh levels and thus to gain information about possible biological functions and regulation by photoperiod. the hhgh gene was cloned and its amino acid sequence deduced from the cdna. the mature hhgh protein consists of 186 amino acids. comparison with other flatfi ... | 2002 | 12161207 |
| retinoic acid isomers produce malformations in postembryonic development of the japanese flounder, paralichthys olivaceus. | we previously reported that characteristic deformities were induced by retinoic acid (ra) treatment of the japanese flounder, paralichthys olivaceus, at 6-9 days post-hatching (dph). to evaluate the toxic potency of nuclear retinoid receptors in induction of deformities by ra, we here investigated the effects of retinoic acid isomers on postembryonic development of this species. larvae were exposed to either 25 nm of all-trans ra (atra), 9-cis ra (9cra) or 13-cis ra (13cra) at 6-9 dph. all ra is ... | 2002 | 12426472 |
| a homologue of human placental protein, pp11, and mouse t cell-specific protein, tcl-30, in exocrine pancreas of a teleost (paralichthys olivaceus). | we cloned a 1401-bp cdna encoding a novel pancreatic protein (ppsb) with two cysteine-rich somatomedin b-like domains from japanese flounder (paralichthys olivaceus). ppsb is predicted to be composed of 385 amino acids, including a signal sequence. the peptide sequence shares high homology with human placental protein 11 (pp11) and mouse t-cell specific protein (tcl-30), which both contain a single somatomedin b-like domain. ppsb shares 47% and 44% identity with pp11 and tcl-30, respectively. th ... | 2002 | 12431400 |
| probiotic effect of lactobacillus sp. ds-12 in flounder (paralichthys olivaceus). | pea aphids were reared aseptically in the laboratory and fed on strains of erwinia aphidicola, erwinia herbicola, klebsiella pneumoniae, escherichia coli and bacterium w, an aphid enterobacterium, by mixing with a synthetic diet to determine whether these bacteria have pathogenicity to the insect. it turned out that er. aphidicola and er. herbicola grew in aphid gut, while k. pneumoniae, es. coli and bacterium w were effectively eliminated. in the case of er. aphidicola, ingestion of a single do ... | 1997 | 12501308 |
| complete genome sequence of lymphocystis disease virus isolated from china. | lymphocystis diseases in fish throughout the world have been extensively described. here we report the complete genome sequence of lymphocystis disease virus isolated in china (lcdv-c), an lcdv isolated from cultured flounder (paralichthys olivaceus) with lymphocystis disease in china. the lcdv-c genome is 186,250 bp, with a base composition of 27.25% g+c. computer-assisted analysis revealed 240 potential open reading frames (orfs) and 176 nonoverlapping putative viral genes, which encode polype ... | 2004 | 15194775 |
| probiotic effect of lactobacillus sp. ds-12 in flounder (paralichthys olivaceus). | | 1997 | 12501318 |
| a conserved 37 kda outer membrane protein of edwardsiella tarda is an effective vaccine candidate. | an effective vaccine against edwardsiella tarda has not been reported, one of main reasons is the variation in its serotypes. this study aimed to develop an effective vaccine against different serotypes of e. tarda. a conserved 37 kda outer membrane protein (omp) of e. tarda was obtained by sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis (page). it showed comprising several proteins by two-dimensional (2d) page analysis and showed a high similarity to glyceraldehyde-3-phosphate d ... | 2004 | 15308366 |
| specific probiotic characterization of weissella hellenica ds-12 isolated from flounder intestine. | a total of 199 microorganisms were isolated from the intestinal contents of flounder (paralichthys olivaceus) in a fish farm in seoul, korea. among these strains, ds-12 was selected as a candidate for flounder probiotics because of its excellent exhibition of antimicrobial activity against fish pathogens such as edwardsiella, pasteurella, aeromonas, and vibrio, and initiate growth in 10% nacl, 10% bile, and in broth at ph 3 for 90 min. this strain was gram-positive, and catalase-negative coccoid ... | 1998 | 12501410 |
| inhibition of chemiluminescent response of olive flounder paralichthys olivaceus phagocytes by the scuticociliate parasite uronema marinum. | experiments were conducted to evaluate the in vitro capacity of the scuticociliatian parasite uronema marinum to inhibit chemiluminescence (cl) of olive flounder paralichthys olivaceus phagocytes. luminol-enhanced cl was used to measure the production of reactive oxygen intermediates (rois) generated by respiratory bursts of phagocytes using zymosan as a stimulant. cytotoxic and antioxidative activities of excretory-secretory (es) products of the parasite were evaluated as well. live u. marinum ... | 2002 | 12542088 |
| molecular cloning of the beta subunit of complement component eight of rainbow trout. | complement-mediated killing of pathogens through the lytic pathway is an important effector mechanism of the innate immune response. c8 is one of the components of the lytic pathway and is composed of an alpha, beta, and gamma subunit. in the present study we report the cloning and characterization of the primary structure of the c8beta subunit in the rainbow trout (oncorhynchus mykiss). the deduced amino acid sequence of trout c8beta shows 72 and 47% identity with that of japanese flounder and ... | 2003 | 12590968 |
| molecular cloning of the seventh component of complement in rainbow trout. | c7 is an integral component of the lytic pathway of complement, which interacts in a sequence of polymerization reactions with other terminal components to form the membrane attack complex. c7 plays a central role in the terminal complement cascade, since its incorporation into the nascent complex allows its hydrophilic-amphiphilic transition, which subsequently leads to the direct binding of the complex to the target membrane. to date, only human and porcine c7 have been cloned and characterize ... | 2005 | 15450749 |
| isolation and characterization of a novel cxc chemokine in common carp (cyprinus carpio l.). | a novel cxc chemokine was identified for the first time in fish from common carp (cyprinus carpio l.). the gene was obtained from the head kidney (hk) stimulated with lps and con a. the cdna consists of 619 bp with a 37 bp 5' utr and a 287 bp 3' utr. an open reading frame of 368 bp encodes a 97 amino acid peptide, with a putative signal peptide of 20 aa. the gene has four cysteines residues, which are conserved, with first two cysteines separated with phenylalanine. by homology and phylogenetic ... | 2003 | 12617998 |
| cloning and characterization of cdnas for two distinct tumor necrosis factor receptor superfamily genes from japanese flounder paralichthys olivaceus. | tumor necrosis factor receptor (tnfr) superfamily regulates diverse biologic functions, including cell proliferation, differentiation, and survival, in addition to providing costimulatory signals for programmed cell death or apoptosis. in this study, cdna fragments for two distinct tnfr homologues were obtained from a japanese flounder, paralichthys olivaceus, cdna library. full-length cdnas of tnfr-1 and tnfr-2 homologues were obtained by using these cdna fragments as probes. the cdna for the j ... | 2003 | 12631519 |
| the spotted wolffish (anarhichas minor olafsen) complement component c3: isolation, characterisation and tissue distribution. | the complement component c3 was isolated from spotted wolffish (anarhichas minor olafsen) serum by polyethylene glycol precipitation, anion exchange chromatography and gel filtration. silver staining in sds-page and rabbit anti-wolffish c3 antiserum used in western blotting revealed that spotted wolffish c3 contains two polypeptide chains, m(r)65 and 115kda, respectively. the high molecular weight alpha-chain of the c3 incorporated 14c-methylamine suggesting that it contained a reactive thioeste ... | 2003 | 12787684 |
| molecular cloning and cdna sequence analysis of carboxypeptidases a1, a2 and b from the japanese flounder paralichthys olivaceus. | although pancreatic serine proteases have been cloned in teleosts, no sequence data are currently available on members of the carboxypeptidase (cp) family. here, we cloned cdnas coding for two preprocpas, corresponding to mammalian preprocpa1 and preprocpa2, and one preprocpb from a pancreatic cdna library of the japanese flounder, paralichthys olivaceus. the activation peptides of flounder procps completely retained the sequences for inhibition of enzymatic activity of procps just like mammalia ... | 2003 | 12892751 |
| comparison of the acid-base responses to co2 and acidification in japanese flounder (paralichthys olivaceus). | to investigate whether the biological toxicity of aquatic hypercapnia is due to the direct effects of co2 or to the effects of acidification of seawater by co2, the japanese flounder (paralichthys olivaceus) was subjected to seawater equilibrated with a gas mixture of air containing 5% co2 (ph 6.18) or seawater acidified to the same ph with 1 n h2so4. all the fish died within 72 h in the co2 exposure group, whereas no mortality occurred in the acid group. acid-base parameters as well as plasma i ... | 2004 | 15556193 |
| cryopreservation of flounder (paralichthys olivaceus) sperm with a practical methodology. | a simple and convenient protocol for the cryopreservation of the flounder (paralichthys olivaceus) sperm was established for "on the spot" cryopreservation of large quantities of semen. the use of three cryoprotectants, dimethyl sulphoxide (dmso), glycerol (gly) and methanol was tested in the method. the percentage of motile sperm present in semen after it had been frozen and thawed in the presence of dmso, gly or methanol was 60.5+/-3.6, 79.17+/-4.5 and 13.25+/-4.7%, respectively. the fertiliza ... | 2003 | 12935875 |
| pseudocohnilembus persalinus (ciliophora: scuticociitida) is an additional species causing scuticociliatosis in olive flounder paralichthys olivaceus. | in the present study, pseudocohnilembus persalinus was first reported as a species causing scuticociliatosis in olive flounder paralichthys olivaceus. based on the stained specimens, p. persalinus was clearly differentiated from uronema marinum, which has been shown to be a cause of scuticociliatosis in farmed olive flounder in korea from its characteristic oral infraciliature structure. the 1754 bp small subunit ribosomal rna (ss rrna) gene sequence of p. persalinus showed 95% homology with the ... | 2004 | 15672880 |
| effects of bile acids on proliferation and production of proteinase activity of uronema marinum (ciliophora: scuticociliatida). | little is known about the effects of bile acids in relation to infectivity on the biological characteristics of uronema marinum, a serious opportunistic parasite of farmed olive flounder paralichthys olivaceus. in this study, we examined the effects of bile acids on the proliferation of u. marinum and on proteinase production in vitro. proliferation of u, marinum was significantly enhanced by lithocholic acid (lca) at 30 and 60 pmol, and by chenodeoxycholic acid (cdca) at 0.06 pmol. in contrast, ... | 2004 | 15672881 |
| susceptibility of cultured juveniles of several marine fish to the sevenband grouper nervous necrosis virus. | piscine nodaviruses (betanodaviruses) have been tentatively divided into four genotypes (sjnnv, rgnnv, tpnnv and bfnnv) and it is suggested that host specificity is different among these genotypes. in the present study, a betanodavirus [sevenband grouper nervous necrosis virus (sgnnv)] belonging to the redspotted grouper nervous necrosis virus (rgnnv) genotype, to which most betanodaviruses from warm water fish are identified, was evaluated for its pathogenicity to hatchery-reared juveniles of s ... | 2003 | 12962219 |
| mucus ph of the tiger puffer takifugu rubripes is an important factor for host identification by the monogenean heterobothrium okamotoi. | we examined a host-finding factor of the monogenean heterobothrium okamotoi oncomiracidia to develop an alternative prophylaxis. h. okamotoi oncomiracidia attached preferentially to gill filaments and skin mucus from the tiger puffer takifugu rubripes compared with corresponding material from other tested fishes (amber jack seriola dumerili, red sea bream pagrus major, japanese flounder paralichthys olivaceus and spotted halibut verasper variegatus). the body mucus ph of the tiger puffer was 6.4 ... | 2003 | 12964825 |
| occurrence of two distinct types of tissue inhibitor of metalloproteinases-2 in teleost fish. | we have cloned for the first time two cdnas encoding distinct types of tissue inhibitor of metalloproteinases-2 (timp-2) from teleost fish, japanese flounder, and designated these types as jftimp-2a and jftimp-2b. the open reading frames of the jftimp-2a and jftimp-2b cdnas are composed of 663 and 657 nucleotides and 221 and 219 amino acids, respectively. both jftimp-2s contain 12 cysteine residues, which might form six disulfide bonds as in other animals' timp-2s. the predicted full-length amin ... | 2003 | 14522085 |
| cpg motif in synthetic odn primes respiratory burst of olive flounder paralichthys olivaceus phagocytes and enhances protection against edwardsiella tarda. | effects of synthetic cytidine-phosphate-guanosine (cpg) oligodeoxynucleotide (odn) on respiratory burst activity of olive flounder (paralichthys olivaceus) head-kidney phagocytes and on protection against lethal infection with edwardsiella tarda were investigated. phagocytes precultured with a cpg odn showed significantly higher chemiluminescence (cl) responses than phagocytes precultured with guanosine-phosphate-cytidine (gpc) odn or culture medium alone (control) at all concentrations. superna ... | 2003 | 14524500 |
| in vitro inhibition of fish rhabdoviruses by japanese flounder, paralichthys olivaceus mx. | a homologous fish cell line stably expressing the recombinant japanese flounder mx (jfmx) was infected with hirame rhabdovirus (hirrv) and viral hemorrhagic septicemia virus (vhsv), both of which are negative single-stranded rna viruses belonging to the rhabdoviridae family. analysis of primary transcription of the two rhabdoviruses showed that there was lower expression level and copy number of the viral nucleoprotein transcript in the jfmx-transfected cell line than the infected, control cells ... | 2003 | 14698674 |
| establishment and characterization of two new cell lines derived from flounder, paralichthys olivaceus (temminck & schlegel). | two new cell cultures from flounder, paralichthys olivaceus (temminck & schlegel), flounder fin (ffn) cells from fin tissue and flounder spleen (fsp) cells from spleen tissue, were established and characterized. the cells multiplied well in eagle's minimum essential medium, supplemented with 10% foetal bovine serum, and have been subcultured more than 100 times, becoming continuous cell lines. modal diploid chromosome number of ffn and fsp cells was 64 and 62, respectively. polymerase chain reac ... | 2003 | 14710758 |
| european sea bass (dicentrarchus labrax l.) cytochrome p450arom: cdna cloning, expression and genomic organization. | cytochrome p450arom, a key enzyme in the hormonal steroidogenic pathway, mediates the conversion of androgens to estrogens. this work describes the molecular cloning of the cdna encoding the european sea bass (dicentrarchus labrax l.) cytochrome p450arom by means of reverse transcriptase and polymerase chain reaction (rt-pcr) and 5' and 3'-rapid amplification of cdna ends (race) analyses. the cdna is 1822bp in length and encodes a putative protein of 517 amino acids. northern blot analysis revea ... | 2002 | 11867261 |
| characterization of gene structure and expression of two toll-like receptors from japanese flounder, paralichthys olivaceus. | we cloned the cdnas and genes of two different types of toll-like receptors from japanese flounder. the results of homology searches suggested that these genes (designated jf-tlr2 and jf-tlr22) are homologues of human tlr2 and fugu tlr22, respectively. the cdnas of jf-tlr2 and jf-tlr22 encoded 818 and 961 amino acid residues, respectively. jf-tlr2 and jf-tlr22 contained two distinct structural/functional motifs of the tlr family, such as a leucine-rich repeat (lrr) domain in the extracellular po ... | 2004 | 15007570 |
| distribution of marine birnavirus in cultured marine fish species from kagawa prefecture, japan. | to determine the distribution of marine birnavirus (mabv) in cultured populations of different marine fish species, 1291 pooled tissue samples from 2672 fish belonging to 22 species and one hybrid were collected from kagawa prefecture, japan, during 1999-2001. using cell-culture mabv was isolated from three species: yellowtail, seriola quinqueradiata temminck & schlegel (positive number/sample number, 10/419), amberjack, s. dumerili (risso) (4/72), and japanese flounder, paralichthys olivaceus ( ... | 2004 | 15009250 |
| experimental horizontal transmission of viral hemorrhagic septicemia virus (vhsv) in japanese flounder paralichthys olivaceus. | infection by viral hemorrhagic septicemia virus (vhsv) has recently occurred among wild and farmed japanese flounder paralichthys olivaceus in japan. in the present study, horizontal transmission of vhsv among japanese flounder was experimentally demonstrated by immersion challenge. exposure to a flounder isolate (obama25) of vhsv revealed a dose-response, with higher mortality (81 and 70%) at the 2 higher exposure levels (6.0 and 4.0 log10 tcid50 ml(-1)). in a second experiment, high titers of ... | 2004 | 15109132 |
| comparison of toxic effects of nitric oxide and peroxynitrite on uronema marinum (ciliata: scuticociliatida). | to discover the effects of nitric oxide (no) and peroxynitrite on uronema marinum (a ciliate responsible for systemic scuticociliatosis in cultured olive flounder paralichthys olivaceus), the dose-dependent inhibitory effect of no donors, s-nitroso-n-acetylpenicillamine (snap) and 3-morpholinosydnonimine (sin-1) on the proliferation and survival of u. marinum was investigated. the inhibitory effects of exogenous superoxide dismutase (sod) and catalase on the toxicity of sin-1 were also investiga ... | 2004 | 15109150 |
| in vivo and in vitro analysis of the resistance against viral haemorrhagic septicaemia virus in japanese flounder (paralichthys olivaceus) precedingly infected with aquabirnavirus. | the resistance of japanese flounder (paralichthys olivaceus temminck et schlegel) against a viral haemorrhagic septicaemia virus (vhsv) challenge induced by a preceding non-lethal aquabirnavirus (abv) challenge was investigated through experimental dual-infections with different intervals between the two challenges. the non-specific protection conferred by the primary abv infection against the secondary vhsv infection commenced at day 3 and persisted up to day 14 but vanished at day 21 post-abv ... | 2004 | 15145413 |
| characterization of structure and expression of the growth hormone receptor gene of the japanese flounder (paralichtys olivaceus). | growth hormone receptor (ghr) cdna and gene of the japanese flounder (paralicthys olivaceus) were cloned and their molecular structures were characterized. the 641 amino acid sequence predicted from the cdna sequence showed more than 75% overall sequence similarity with ghrs of other teleosts such as turbot and goldfish, and contained common structural features of vertebrate ghrs. the extracellular domain of flounder ghr had three pairs of cysteines and an fgefs motif with a replacement e to d. ... | 2004 | 15225140 |
| development of a dna vaccine against hirame rhabdovirus and analysis of the expression of immune-related genes after vaccination. | intramuscular injection of japanese flounder, paralichthys olivaceus (average weight approximately 2 g) with 1 and 10 microg of a plasmid dna vaccine encoding the hirame rhabdovirus (hirrv) glycoprotein gene (pcmv-hrvg) was found to provide strong protection against hirrv. we also conducted a real-time pcr analysis to quantify immune-related genes, e.g. mhc class ialpha, iialpha, iibeta, tcr-alpha, beta1, beta2 and delta, to characterize the immune response at 1 and 7 days after dna vaccination. ... | 2004 | 15312663 |
| a pore-forming protein, perforin, from a non-mammalian organism, japanese flounder, paralichthys olivaceus. | a perforin cdna of japanese flounder, paralichthys olivaceus, was cloned from a cdna library of kidney stimulated with cona/pma. the full-length cdna is 2,157 bp, which encodes 587 amino acids. the japanese flounder perforin gene consists of five exons and four introns, with a length of approximately 3 kb. the amino acid sequence of the japanese flounder perforin is 36% identical to that of rat perforin and 37% identical to amino acid sequences of mouse and human perforin. the japanese flounder ... | 2004 | 15322778 |
| protection of flounder against hirame rhabdovirus (hirrv) with a dna vaccine containing the glycoprotein gene. | hirame rhabdovirus (hirrv) is an important virus of cultured flounder (paralichthys olivaceus). we tested the protective immunogenicity of dna-based vaccines against this virus. genes encoding the nucleocapsid protein (n) and the c-terminal half of the glycoprotein (g) were amplified by rt-pcr and separately cloned into the eukaryotic expression vector pcdna 3.1. the g protein expressed by transfected cells was detected by western blot analysis. pcr analyses demonstrated the presence of injected ... | 2006 | 16176849 |
| comparative immune responses in japanese flounder, paralichthys olivaceus after vaccination with viral hemorrhagic septicemia virus (vhsv) recombinant glycoprotein and dna vaccine using a microarray analysis. | a viral hemorrhagic septicemia virus (vhsv), recombinant glycoprotein vaccine (vhsvg) and a dna vaccine (pcmv-vhsg) were injected in japanese flounder (paralichthys olivaceus). each fish was injected with 10 microg vaccine dissolved in 50 microl phosphate buffer saline (pbs). one month after the vaccination, the fish were challenged intraperitoneally with either 1 x 10(2) or 1 x 10(3) tcid(50) of virus. fish that received the vhsg dna vaccine were highly protected against virus infection over a ... | 2006 | 16185791 |
| bacterial enteritis and the development of the larval digestive tract in olive flounder, paralichthys olivaceus (temminck & schlegel). | three bacterial isolates obtained from diseased olive flounder larvae, paralichthys olivaceus, were identified as vibrio ichthyoenteri based on the results of phenotypic characterization and 16s rrna gene sequencing studies. bacterial enteritis was reproduced in 16 and 22 days post-hatch (dph) larvae by administering brine shrimp nauplii, artemia salina, dosed with the environmental isolates and reference strains of v. ichthyoenteri. to investigate the effect of the disease on development of the ... | 2004 | 15357708 |
| molecular characterization of the japanese flounder, paralichthys olivaceus, cd3epsilon and evolution of the cd3 cluster. | a second cd3 gene, i.e. cd3epsilon, has been cloned and sequenced in japanese flounder. the full length cdna is 1006 bp and encodes 164 amino acids. when compared with other known cd3epsilon peptide sequences, the most conserved region of the japanese flounder cd3epsilon chain peptide is the cytoplasmic domain and the least conserved is the extracellular domain. a phylogenetic analysis based on the deduced amino acid sequence grouped the two japanese flounder cd3 sequences with cd3epsilon and cd ... | 2005 | 15450752 |
| characterization of monoclonal antibodies against heavy and light chains of flounder (paralichthys olivaceus) immunoglobulin. | flounder (paralichthys olivaceus) immunoglobulins (igs) were purified from the serum of mouse igg-immunized flounder by using affinity chromatography. under denaturing conditions in sds-page, the flounder igs appeared to be composed of 2 heavy (h) chains (72 and 77 kda) and two light (l) chains (26 and 28 kda). monoclonal antibodies (mabs) were produced by the fusion of myeloma cells (sp2/0) with balb/c mouse spleen cells that were previously sensitized against affinity-purified flounder igs. in ... | 2004 | 15469712 |
| use of a cdna microarray to study immunity against viral hemorrhagic septicemia (vhs) in japanese flounder (paralichthys olivaceus) following dna vaccination. | japanese flounder, paralichthys olivaceus juveniles were vaccinated against viral hemorrhagic septicemia (vhs) by intramuscular injection of 10 microg of a plasmid dna vector which encodes the viral hemorrhagic septicemia virus (vhsv) glycoprotein (g) gene under the control of the cytomegalovirus promoter. experimental challenge of two viral doses (1 x 10(2) tcid50 and 1 x 10(3) tcid50) one month post-vaccination revealed that the g gene was able to induce protective immunity against vhs and thi ... | 2005 | 15475310 |
| establishment of a continuous embryonic cell line from japanese flounder paralichthys olivaceus for virus isolation. | a continuous cell line, the flounder embryonic cell line (fec), was established from gastrula-stage embryos of a marine cultured fish, the japanese flounder paralichthys olivaceus and cultured for more than 200 d with more than 60 passages. fec cells were cultured in dulbecco's modified eagle's medium (dmem) supplemented with antibiotics, fetal bovine serum (fbs), sea perch serum (sps), and basic fibroblast growth factor (bfgf). the cells were small and round, and grew actively and stably in cul ... | 2004 | 15521323 |
| occurrence of scuticociliatosis in olive flounder paralichthys olivaceus by phiasterides dicentrarchi (ciliophora: scuticociliatida). | in the course of identifying scuticociliates recently obtained from systemically infected olive flounder paralichthys olivaceus in korea, we found a scuticociliate species whose small subunit ribosomal rna (ss rrna) gene was not amplified by species-specific primers previously designed for uronema marinum and pseudocohnilembus persalinus. by studying morphological characteristics of wet-mounted and stained specimens, we identified the species as philasterides dicentrarchi, which has been reporte ... | 2004 | 15672879 |
| genetic relationships among members of the ichthyobodo necator complex: implications for the management of aquaculture stocks. | abstract ichthyobodo necator (costia) is a common and important flagellate parasite that infests the skin and gills of many freshwater and marine fish. costia infestations are often fatal and cause significant aquaculture losses worldwide. recently it has been demonstrated that ichthyobodo is a multispecies complex with differing host preferences. knowing if those species have broad or narrow host specificity has important implications for the management of costia. to address the question of hos ... | 2005 | 15705156 |
| immunoanalysis of antiviral mx protein expression in japanese flounder (paralichthys olivaceus) cells. | mx proteins are interferon-inducible gtpases that possess antiviral properties in vertebrates. japanese flounder (paralichthys olivaceus) mx protein has previously been shown to possess some antiviral activity against rhabdoviruses. a polyclonal antibody was generated against a purified peptide fragment of japanese flounder mx protein that had been produced in an escherichia coli expression system. the pab detected the approximately 71 kda mx protein from japanese flounder (hirame) natural embry ... | 2004 | 15707665 |
| biochemical changes as enzymatic defense system of phenanthrene exposure in olive flounder, paralichthys olivaceus. | the objective of this study was to investigate early biological response in olive flounder exposed to sub-lethal concentrations of waterborne phenanthrene (0.5, 1 or 2 microm). the fish were exposed for 4 weeks and we analyzed their enzymatic defense system, antioxidant and phase ii enzyme activities, to evaluate the chronic exposure toxicity of phenanthrene. waterborne phenanthrene affected antioxidant enzymes and glutathione-mediated detoxification as enzyme defense system. hepatic, gill and k ... | 2005 | 15717855 |
| induction of antiviral state in fish cells by japanese flounder, paralichthys olivaceus, interferon regulatory factor-1. | interferon regulatory factor-1 (irf-1) mediates an antiviral state in cells by regulating the expression of the interferon (ifn-alpha/beta) system. to elucidate the role of irf-1 in fish during virus infections, we constructed a recombinant plasmid of the japanese flounder, paralichthys olivaceus irf-1 (jf irf-1) under the control of the cytomegalovirus (cmv) immediate/early enhancer promoter. the antiviral mechanism of jf irf-1 was studied using transfection experiments in a homologous cell lin ... | 2005 | 15722233 |
| differentiation of lymphocystis disease virus genotype by multiplex pcr. | lymphocystis disease virus (lcdv) is the causative agent of lymphocystis disease. the viruses have been divided into three genotypes (genotype i for lcdv-1, ii for japanese flounder isolates, and iii for rockfish isolates) on the basis of major capsid protein (mcp) gene sequences. in this study, we developed a multiplex pcr primer set in order to distinguish these genotypes. we also analyzed the mcp gene of a new lcdv isolate from the sea bass (sb98yosu). comparison of sequence identities betwee ... | 2006 | 16728963 |
| expression profiling of immune-related genes from japanese flounder paralichthys olivaceus kidney cells using cdna microarrays. | a japanese flounder paralichthys olivaceus cdna microarray containing 871 unique cdnas including 91 putative immune-related genes from our est studies was constructed and used to characterize of gene expression of in vitro grown kidney cells stimulated with mitogens such as cona, pma, lps or infected with hirame rhabdovirus (hrv). the numbers of genes whose expressions were increased or decreased by these factors were: 17 by con a, 139 by pma, 76 by lps and 182 by hrv infection. the treatment of ... | 2005 | 15752548 |
| identification of a novel japanese flounder (paralichthys olivaceus) cc chemokine gene and an analysis of its function. | a cdna of japanese flounder (paralichthys olivaceus) cc chemokine designated as paol-scya104 was cloned and sequenced. the cdna contains an opening reading frame of 315 nucleotides encoding 104 amino acid residues. the full gene was cloned and sequenced from a bac library. it has a length of approximately 750 bp from the start codon to the stop codon and is composed of four exons and three introns. four cysteine residues are conserved in the same positions as those of mammalian and fish cc chemo ... | 2004 | 14722688 |
| the primary structure of a novel goose-type lysozyme from rhea egg white. | g-type lysozyme is a hydrolytic enzyme sharing a similar tertiary structure with plant chitinase. to discover the relation of function and structure, we analyzed the primary structure of new g-type lysozyme. the complete 185 amino acid residues of lysozyme from rhea egg white were sequenced using the peptides hydrolyzed by trypsin, v8 protease, and cyanogen bromide. rhea lysozyme had sequence similarity to ostrich, cassowary, goose, and black swan, with 93%, 90%, 83%, and 82%, respectively. the ... | 2004 | 14745179 |
| concentrations of polychlorinated dibenzo-p-dioxins, dibenzofurans, non-ortho polychlorinated biphenyls, and mono-ortho polychlorinated biphenyls in japanese flounder, with reference to the relationship between body length and concentration. | the concentrations of polychlorinated dibenzo-p-dioxins (pcdds), polychlorinated dibenzofurans (pcdfs), non-ortho polychlorinated biphenyls (non-ortho pcbs), and mono-ortho polychlorinated biphenyls (mono-ortho pcbs) in japanese flounder (paralichthys olivaceus, body length 10.4-36.6 cm) collected from sendai bay, japan, were determined using high-resolution gas chromatography-mass spectrometry. the relationship between the concentrations of these compounds (dioxins) and the body length of the j ... | 2004 | 14999318 |
| molecular identification, polymorphism, and expression analysis of major histocompatibility complex class iia and b genes of turbot (scophthalmus maximus). | major histocompatibility complex (mhc) class ii has a central role in the adaptive immune system by presenting foreign peptides to the t-cell receptor. the full lengths of mhc class ii a and b cdna were cloned from turbot by homology cloning and rapid amplification of cdna ends polymerase chain reaction (race pcr), and genomic organization, molecular polymorphism, and expression of turbot class iib gene were examined to study the function of class iib gene in fish. the deduced amino acid sequenc ... | 2006 | 16832747 |