| a parasite survey of farmed southern bluefin tuna, thunnus maccoyii (castelnau). | farmed southern bluefin tuna (sbt) were examined for parasites. samples of harvest fish, mortalities and some fish showing clinical signs of disease were investigated. targeted screening was conducted for a scuticociliate, uronema nigricans, the myxosporean kudoa sp. and a sanguinicolid digenean, cardicola forsteri. general parasitological investigation revealed a diverse parasite community of didymozoid digeneans, two species of copepods, a polyopisthocotylean monogenean and larval cestodes. ta ... | 2005 | 15892753 |
| purification and properties of a glutathione peroxidase from southern bluefin tuna (thunnus maccoyii) liver. | a glutathione peroxidase (gpx) protein was purified approximately 1000-fold from southern bluefin tuna (thunnus maccoyii) liver to a final specific activity of 256 micromol nadph oxidised min(-1) mg(-1) protein. gel filtration chromatography and denaturing protein gel electrophoresis of the purified preparation indicated that the protein has a native molecular mass of 85 kda and is most likely a homotetramer with subunits of approximately 24 kda. the km values of the purified enzyme for hydrogen ... | 2006 | 16540359 |
| concurrent epizootic hyperinfections of sea lice (predominantly caligus chiastos) and blood flukes (cardicola forsteri) in ranched southern bluefin tuna. | peaks in epizootics of sea lice (mostly caligus chiastos) and blood flukes (cardicola forsteri) among southern bluefin tuna (thunnus maccoyii) appear to coincide with the onset of an increased mortality. the mortality event occurs 6-12 weeks after t. maccoyii have been transferred into static ranching pontoons from the wild. however, to date available data on parasite occurrence before commercial harvesting begins, are scant. this research gathered epizootiological data from weeks 4 to 13 post-t ... | 2010 | 20605071 |
| permanent genetic resources added to molecular ecology resources database 1 october 2010-30 november 2010. | this article documents the addition of 277 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: ascochyta rabiei, cambarellus chapalanus, chionodraco hamatus, coptis omeiensis, cynoscion nebulosus, daphnia magna, gerbillus nigeriae, isurus oxyrinchus, lates calcarifer, metacarcinus magister, oplegnathus fasciatus, pachycondyla verenae, phaethon lepturus, pimelodus grosskopfii, rotylenchulus reniformis, scomberomorus niphonius, sep ... | 2011 | 21429157 |
| test of an antifouling treatment on tuna fish-cages in boston bay, port lincoln, south australia. | a test of the antifouling properties of wattyl netclear was conducted on fish farms containing southern bluefin tuna (thunnus maccoyii) during a 6-month period (february-july 2002). wattyl netclear is a water-based synthetic latex-based coating where the active antifouling agent is a mixture of two isothiazolinones likely to affect both biochemical conditioning and bacterial colonisation. the development of fouling was monitored using underwater stereo-photogrammetry on 3 treated and 3 untreated ... | 2006 | 17290865 |
| an epizootic of caligus chiastos on farmed southern bluefin tuna thunnus maccoyii off south australia. | in some years, large numbers of caligus chiastos have been observed on the external surfaces of southern bluefin tuna, particularly on the head and eyes, in some sea cages in spencer gulf, australia. as no epidemiological data were available, we monitored sea lice on tuna (n = 130) in 4 research cages sampled at 6 wk intervals during the 2005 farming season. no lice were observed on a sample of 10 wild-caught tuna when the cohort was transferred to cages in early april. by late may more than hal ... | 2008 | 18429442 |
| cell proliferation and long chain polyunsaturated fatty acid metabolism in a cell line from southern bluefin tuna (thunnus maccoyii). | southern bluefin tuna (sbt, thunnus maccoyii) aquaculture is a highly valuable industry, but research on these fish is hampered by strict catch quotas and the limited success of captive breeding. to address these limitations, we have developed a sbt cell line (sbt-e1) and here we report on fatty acid metabolism in this cell line. the sbt-e1 cells proliferated well in standard leibovitz's l-15 cell culture medium containing fetal bovine serum (fbs) as the source of fatty acids. decreasing the fbs ... | 2014 | 24825740 |
| the kiss2 receptor (kiss2r) gene in southern bluefin tuna, thunnus maccoyii and in yellowtail kingfish, seriola lalandi - functional analysis and isolation of transcript variants. | the kisspeptin system plays an essential role in reproductive function in vertebrates, particularly in the onset of puberty. we investigated the kisspeptin system in two perciform teleosts, the southern bluefin tuna (sbt; thunnus maccoyii), and the yellowtail kingfish (ytk; seriola lalandi), by characterising their kisspeptin 2 receptor (kiss2r) genes. in addition to the full length kiss2r cdna sequences, we have isolated from sbt and ytk a transcript variant that retained an intron. we have fur ... | 2012 | 22824208 |
| assessment of yellowtail kingfish (seriola lalandi) as a surrogate host for the production of southern bluefin tuna (thunnus maccoyii) seed via spermatogonial germ cell transplantation. | germ cell transplantation is an innovative technology for the production of interspecies surrogates, capable of facilitating easier and more economical management of large-bodied broodstock, such as the bluefin tuna. the present study explored the suitability of yellowtail kingfish (seriola lalandi) as a surrogate host for transplanted southern bluefin tuna (thunnus maccoyii) spermatogonial cells to produce tuna donor-derived gametes upon sexual maturity. germ cell populations in testes of donor ... | 2015 | 26195109 |
| the influence of finfish aquaculture on benthic fish and crustacean assemblages in fitzgerald bay, south australia. | the influence of sea-cage aquaculture on wildfish assemblages has received little attention outside of europe. sea-cage aquaculture of finfish is a major focus in south australia, and while the main species farmed is southern bluefin tuna (thunnus maccoyii), there is also an important yellowtail kingfish (seriola lalandi) industry. yellowtail kingfish aquaculture did not appear to have any local or regional effects on demersal assemblages (primarily fish, but also some crustaceans) surveyed by b ... | 2015 | 26401452 |
| inferring the nature of anthropogenic threats from long-term abundance records. | diagnosing the processes that threaten species persistence is critical for recovery planning and risk forecasting. dominant threats are typically inferred by experts on the basis of a patchwork of informal methods. transparent, quantitative diagnostic tools would contribute much-needed consistency, objectivity, and rigor to the process of diagnosing anthropogenic threats. long-term census records, available for an increasingly large and diverse set of taxa, may exhibit characteristic signatures ... | 2015 | 25065712 |
| culturable microbiota of ranched southern bluefin tuna (thunnus maccoyii castelnau). | the australian tuna industry is based on the ranching of wild southern bluefin tuna (sbt, thunnus maccoyii). within this industry, only opportunistic pathogens have been reported infecting external wounds of fish. this study aimed to identify different culturable bacteria present in three cohorts of sbt and to determine normal bacteria and potential pathogens in isolates from harvest fish and moribund/dead fish. post-mortem changes in the microbiota were also studied. | 2013 | 23789748 |
| factors affecting abundance and prevalence of blood fluke, cardicola forsteri, infection in commercially ranched southern bluefin tuna, thunnus maccoyii, in australia. | a survey of blood fluke, cardicola forsteri, infection in ranched southern bluefin tuna, thunnus maccoyii, was undertaken over three farming seasons, from march 2004 to september 2006. analyses of covariance and logistic regression were used to explore the effects of company, year, season, time in culture, and condition index on intensity, abundance and prevalence of blood fluke infection. average prevalence of blood fluke infection was 62.64% over the period of the survey. average intensity was ... | 2015 | 25862423 |
| moving cages further offshore: effects on southern bluefin tuna, t. maccoyii, parasites, health and performance. | the effects of offshore aquaculture on sbt health (particularly parasitic infections and haematology) and performance were the main aim of this study. two cohorts of ranched southern bluefin tuna (sbt) (thunnus maccoyii) were monitored throughout the commercial season, one maintained in the traditional near shore tuna farming zone and one maintained further offshore. sbt maintained offshore had reduced mortality, increased condition index at week 6 post transfer, reduced blood fluke and sealice ... | 2011 | 21901129 |
| case-control study of epidemic mortality and cardicola forsteri-associated disease in farmed southern bluefin tuna (thunnus maccoyii) of south australia. | cardicola forsteri is a blood fluke that is highly prevalent among cultured southern bluefin tuna (sbt), thunnus maccoyii, in south australia. the role of c forsteri in annual sbt mortality outbreaks, which peak 6 to 12 weeks poststocking, is unknown. the objective of this study was to identify lesions unique to cultured sbt that died during a mortality event in 2009 and to determine the significance of c forsteri-associated lesions. cultured sbt were sampled from 4 pontoons of a single site in ... | 2010 | 21084722 |
| cloning and characterization of two glutathione peroxidase cdnas from southern bluefin tuna (thunnus maccoyii). | glutathione peroxidases (gpxs; ec 1.11.19) are key enzymes in the antioxidant defence systems of living organisms, including fish. here we report the cloning of two gpx cdnas from southern bluefin tuna (sbt, thunnus maccoyii). one encodes a gpx1 or classical gpx and the other encodes a gpx4 or phospholipid hydroperoxide gpx. the 898 base-pair (bp) sbt gpx1 cdna (genbank accession no. ef452497) includes the complete protein coding region plus 35bp of the 5'-untranslated region (5'-utr) and 296bp ... | 2010 | 20416392 |
| cloning and functional characterization of a typical 2-cys peroxiredoxin from southern bluefin tuna (thunnus maccoyii). | peroxiredoxins (prxs, ec: 1.11.1.15) are cysteine-dependent peroxidases proposed to function as antioxidant enzymes and also in h2o2-mediated cell signaling. they have been well characterized in yeast, mammals, protists and bacteria but not yet in fish. here we describe the cloning and functional characterization of a prx 2 cdna from southern bluefin tuna (sbt, thunnus maccoyii), an important aquaculture species in south australia. the sbt prx sequence was closely related (76-92% identical) to p ... | 2010 | 20211754 |
| cloning and functional characterisation of a fatty acyl elongase from southern bluefin tuna (thunnus maccoyii). | the synthesis of long chain polyunsaturated fatty acids (lcpufa), such as eicosapentaenoic acid (epa; 20:5n-3) and docosahexaenoic acid (dha; 22:6n-3), involves fatty acyl desaturase and elongase enzymes. the marine fish species southern bluefin tuna (sbt) can accumulate large quantities of omega-3 (n-3) lcpufa in its flesh but their capacity to synthesize epa and dha is uncertain. a cdna, sbtelovl5, encoding a putative fatty acyl elongase was amplified from sbt liver tissue. the cdna included a ... | 2010 | 19906358 |
| serological evidence of an antibody response in farmed southern bluefin tuna naturally infected with the blood fluke cardicola forsteri. | in this study, adaptive immune response was investigated in farmed southern bluefin tuna, thunnus maccoyii, infected with a sanguinicolid cardicola forsteri. a cohort (cohort(2005)) of southern bluefin tuna was sampled between march 2005 and august 2006. samples were taken at the transfer of wild caught tuna to sea cages and then at regular intervals. parasite intensity, abundance and prevalence data were recorded. an elisa was developed to detect and quantify an antibody response against the bl ... | 2008 | 18502150 |
| dioxins, pcbs, metals, metalloids, pesticides and antimicrobial residues in wild and farmed australian southern bluefin tuna (thunnus maccoyii). | data concerning the concentration of dioxins (pcdd/pcdfs), polychlorinated biphenyls (pcbs), metals, metalloids, pesticides and antimicrobials residues are reported for the edible portion of australian wild and farmed southern bluefin tuna (thunnus maccoyii). in 2004 wild caught (n=5) and farmed (n=26) southern bluefin tuna (sbt) were collected. the mean concentration of total mercury on a fresh weight (fw) basis was 0.34 mg/kg in wild sbt and 0.31 mg/kg in farmed sbt. concentrations of dioxins ... | 2008 | 18395242 |
| epizootics of metazoan gill parasites did not threaten feasibility of farming southern bluefin tuna (thunnus maccoyii) in a trial extending over summer months. | tuna farming off port lincoln, australia, involves catching wild 2-4-year-old southern bluefin tuna in summer and then fattening for periods of 2-8 months. as fresh product is not available year-round, the feasibility of maintaining tuna for longer periods was trialled, including over a summer season, when temperatures may exceed 24 degrees c. as the rates of growth and reproduction in ectoparasites of fishes are usually most rapid during warm temperatures, parasite epizootics at this time may a ... | 2008 | 18394810 |
| gross and histopathological characteristics of two lipomas and a neurofibrosarcoma detected in aquacultured southern bluefin tuna, thunnus maccoyii (castelnau), in south australia. | three visible lesions were examined from two specimens of southern bluefin tuna. the lesions were examined grossly and two were identified as lipomas, the third bore similarities to a schwannoma. histopathology confirmed that two consisted of mature adipocytes consistent with a diagnosis of lipoma. the third lesion consisted of spindle cells in antoni a and b patterns and was tentatively diagnosed as a malignant schwannoma. immunohistochemistry identified both s100 and glial fibrillary acid prot ... | 2008 | 18353015 |
| development and validation of a radioimmunoassay for fish insulin-like growth factor i (igf-i) and the effect of aquaculture related stressors on circulating igf-i levels. | this paper describes the development and validation of a commercially available radioimmunoassay (ria) for the detection of fish insulin-like growth factor-i (igf-i). the assay was developed using recombinant barramundi igf-i as antigen and recombinant tuna igf-i as radiolabelled tracer and standard. assay sensitivity was 0.15 ng/ml, inter-assay variation was 16% (n = 9) and intra-assay variation was 3% (n = 10). cross reactivity of less than 0.01% was found with salmon insulin, salmon igf-ii an ... | 2004 | 14723878 |
| isolation and partial characterisation of immunoglobulin from southern bluefin tuna thunnus maccoyii castelnau. | specific and total serum immunoglobulins were extracted by immunoaffinity, mannan-binding protein and protein a affinity chromatography from southern bluefin tuna (thunnus maccoyii castelnau) immunised with rabbit igg, and from non-immunised southern bluefin tuna. sds-page in 10% reducing gels revealed two heavy chains with molecular weights of approximately 74.6 +/- 1.3 kda and 71.2 +/- 0.9 kda, and two light chains with molecular weights of approximately 29 +/- 1.2 kda and 28 +/- 1.0 kda. unde ... | 2001 | 11556479 |