| cardicola opisthorchis n. sp. (trematoda: aporocotylidae) from the pacific bluefin tuna, thunnus orientalis (temminck & schlegel, 1844), cultured in japan. | a new aporocotylid blood fluke is described, based on specimens from the ventricle of the pacific bluefin tuna, thunnus orientalis (temminck et schlegel), cultured in wakayama and nagasaki prefectures, japan. the new species is morphologically similar to the members of the genus cardicola short, 1953, but shows distinct differences in the body form, location of the testis and the orientation of the ootype. the body of the new species is long and slender, whereas other cardicola species are small ... | 2011 | 21616163 |
| discovery of intermediate hosts for two species of blood flukes cardicola orientalis and cardicola forsteri (trematoda: aporocotylidae) infecting pacific bluefin tuna in japan. | fish blood flukes (aporocotylidae) are important pathogens of farmed finfish around the world. among them, cardicola spp. infecting farmed tuna are considered to be serious threats to tuna farming and have received tremendous attention. we conducted periodical samplings at a tuna farming site in japan between january and may, 2015 to determine the life cycle of cardicola spp. we collected over 4700 terebellid polychaetes from ropes, floats and frames of tuna culture cages and found nearly 400 in ... | 2016 | 26571413 |
| developmental stages of fish blood flukes, cardicola forsteri and cardicola opisthorchis (trematoda: aporocotylidae), in their polychaete intermediate hosts collected at pacific bluefin tuna culture sites in japan. | farming of pacific bluefin tuna (pbt), thunnus orientalis, is a rapidly growing industry in japan. aporocotylid blood flukes of the genus cardicola comprising c. orientalis, c. opisthorchis and c. forsteri are parasites of economic importance for pbt farming. recently, terebellid polychaetes have been identified as the intermediate hosts for all these parasites. we collected infected polychaetes, terebella sp., the intermediate host of c. opisthorchis, from ropes and floats attached to tuna cage ... | 2017 | 27835838 |
| a new blood fluke of the genus cardicola (trematoda: sanguinicolidae) from pacific bluefin tuna thunnus orientalis (temminck & schlegel, 1844) cultured in japan. | a new sanguinicolid blood fluke, cardicola orientalis n. sp., is described from the afferent branchial artery and heart of pacific bluefin tuna thunnus orientalis (temminck & schlegel) cultured in mie and nagasaki prefectures, japan. the new species is most similar to c. ambrosioi braicovich, etchegoin, timi et sardella, 2006 from the brazilian flathead, percophis brasiliensis quoy & gaimard, but can be differentiated by the position of the female genital pore (in midline or slightly sinistral i ... | 2010 | 19835980 |
| permanent genetic resources added to molecular ecology resources database 1 october 2010-30 november 2010. | this article documents the addition of 277 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: ascochyta rabiei, cambarellus chapalanus, chionodraco hamatus, coptis omeiensis, cynoscion nebulosus, daphnia magna, gerbillus nigeriae, isurus oxyrinchus, lates calcarifer, metacarcinus magister, oplegnathus fasciatus, pachycondyla verenae, phaethon lepturus, pimelodus grosskopfii, rotylenchulus reniformis, scomberomorus niphonius, sep ... | 2011 | 21429157 |
| identification of a novel selenium-containing compound, selenoneine, as the predominant chemical form of organic selenium in the blood of bluefin tuna. | a novel selenium-containing compound having a selenium atom in the imidazole ring, 2-selenyl-n(alpha),n(alpha),n(alpha)-trimethyl-l-histidine, 3-(2-hydroseleno-1h-imidazol-5-yl)-2-(trimethylammonio)propanoate, was identified from the blood and other tissues of the bluefin tuna, thunnus orientalis. the selenium-containing compound was purified from the tuna blood in several chromatographic steps. high resolution mass spectrometry and nuclear magnetic resonance spectroscopy showed that the exact m ... | 2010 | 20388714 |
| characterization of thyroid hormones and thyroid hormone receptors during the early development of pacific bluefin tuna (thunnus orientalis). | we studied the profiles of 3,5,3'-l-triiodothyronine (t3), thyroxine (t4), and thyroid hormone receptors (trs) in pacific bluefin tuna (thunnus orientalis) during embryonic and post-embryonic development. both t3 and t4 were detected in embryos just before hatching, and it was found that the levels of both were increased in postflexion fish. the thyroid follicles were increased in both size and number in postflexion fish compared with preflexion fish. a trbeta cdna clone was generated by race. t ... | 2008 | 17988667 |
| modifying effect of tuna orbital oil rich in docosahexaenoic acid and vitamin d3 on azoxymethane-induced colonic aberrant crypt foci in rats. | the modifying effect of dietary tuna (thunnus thynnus orientalis) orbital oil rich in docosahexaenoic acid (dha) and vitamin d3 (vd3) on the development of azoxymethane (aom)-induced colonic aberrant crypt foci (acf) was investigated in male f344 rats. animals were given three weekly subcutaneous injections of aom (15 mg/kg body weight) to induce acf. the rats were fed the experimental diet containing 5% tuna orbital oil (low fish oil), 23.5% tuna orbital oil (high fish oil), 5% corn oil (low co ... | 2000 | 10948340 |
| transplantation of cardicola opisthorchis (trematoda: aporocotylidae) sporocysts into the intermediate host, terebella sp. (polychaeta: terebellidae). | cardicola opisthorchis is a blood fluke pathogen significantly affecting cultured pacific bluefin tuna thunnus orientalis in japan. it is known that the intermediate host of c. opisthorchis is a terebellid polychaete terebella sp. in order to study the intrapolychaete larval development of c. opisthorchis, we transplanted sporocysts, which contained a large number of cercariae, of c. opisthorchis obtained from terebella sp. into sporocyst-free terebella sp., which had been maintained at 20°c. th ... | 2017 | 27693561 |
| larval stages of the bluefin tuna blood fluke cardicola opisthorchis (trematoda: aporocotylidae) found from terebella sp. (polychaeta: terebellidae). | we found aporocotylid larval stages (sporocysts and cercariae) from five individuals of terebellid polychaete terebella sp., which were collected from seabed substrate and ropes and floats attached to tuna cages in a tuna farm on the coast of tsushima island, nagasaki, japan. nucleotide sequences of the regions of internal transcribed spacer 2 ribosomal dna and 28s ribosomal dna from these larval stages were 100% identical to those of cardicola opisthorchis registered in genbank. c. opisthorchis ... | 2014 | 24291605 |
| the pacific bluefin tuna (thunnus orientalis) dead end gene is suitable as a specific molecular marker of type a spermatogonia. | we developed a spermatogonial transplantation technique to produce donor-derived gametes in surrogate fish. our ultimate aim is to establish surrogate broodstock that can produce bluefin tuna. we previously determined that only type a spermatogonia (asg) could colonize recipient gonads in salmonids. therefore, it is necessary to develop a precise molecular marker that can distinguish asg in order to develop efficient spermatogonial transplantation methods. in this study, the pacific bluefin tuna ... | 2013 | 23913406 |
| kudoa prunusi n. sp. (myxozoa: multivalvulida) from the brain of pacific bluefin tuna thunnus orientalis (temminck & schlegel, 1844) cultured in japan. | kudoa prunusi n. sp. (myxozoa; multivalvulida) is described from the brain of pacific bluefin tuna thunnus orientalis cultured in japan. numerous white cysts, up to 0.5mm in size, were found on and in the brain. spores having typically five spore valves and five polar capsules resembled a five-petal cherry blossom in apical view and were conical shape with a round bottom in side view. average spore size was 9.63 (8.5-10.3) μm in width and 7.50 (6.7-8.6) μm in length. the spore dimensions of k. p ... | 2010 | 21112413 |
| genetic heterogeneity of betanodaviruses in juvenile production trials of pacific bluefin tuna, thunnus orientalis (temminck & schlegel). | pacific bluefin tuna, thunnus orientalis (temminck & schlegel), is one of the most important commercially exploited fish species in the world, and juvenile production techniques have been developed for its culture and stock enhancement in japan. however, recent juvenile production has often failed because of the occurrence of viral nervous necrosis caused by betanodaviruses. in this study, we examined the genetic variability of betanodaviruses detected in the diseased juveniles to understand the ... | 2009 | 19538459 |
| isolation and characterization of a genomic fosmid clone containing hspb1 (hsp27) from the pacific bluefin tuna thunnus orientalis. | we constructed a fosmid library of genomic dna from the pacific bluefin tuna thunnus orientalis and isolated a clone containing the complete gene hspb1 (hsp27) from the library. to screen the library, we first obtained a full-length hspb1 cdna by race-pcr. this cdna encodes a putative protein of 202 amino acids. phylogenetic analysis indicated that the protein belongs to the hsp27 family. northern blot analysis demonstrated that the gene transcript was expressed in tuna muscle tissues. using a c ... | 2008 | 21798158 |
| transcriptomic features associated with energy production in the muscles of pacific bluefin tuna and pacific cod. | bluefin tuna are high-performance swimmers and top predators in the open ocean. their swimming is grounded by unique features including an exceptional glycolytic potential in white muscle, which is supported by high enzymatic activities. here we performed high-throughput rna sequencing (rna-seq) in muscles of the pacific bluefin tuna (thunnus orientalis) and pacific cod (gadus macrocephalus) and conducted a comparative transcriptomic analysis of genes related to energy production. we found that ... | 2016 | 26924100 |
| hearing thresholds of swimming pacific bluefin tuna thunnus orientalis. | pacific bluefin tuna (thunnus orientalis) is a highly migratory, commercially valuable species potentially vulnerable to acoustic noise generated from human activities which could impact behavior and fitness. although significant efforts have been made to understand hearing abilities of fishes, the large size and need to continuously swim for respiration have hindered investigations with tuna and other large pelagic species. in this study, pacific bluefin tuna were trained to respond to a pure t ... | 2015 | 25732931 |
| kudoa hexapunctata n. sp. (myxozoa: multivalvulida) from the somatic muscle of pacific bluefin tuna thunnus orientalis and re-description of k. neothunni in yellowfin tuna t. albacares. | since kudoa septempunctata in olive flounder (paralichthys olivaceus) was indicated to cause food poisoning in humans, other kudoa species are suspected to have pathogenic potential. recently, a myxosporean possibly associated with food poisoning in humans consuming raw pacific bluefin tuna, thunnus orientalis, was identified as kudoa neothunni. this is a known causative myxosporean of post-harvest myoliquefaction in yellowfin tuna thunnus albacares. regardless of the significant differences in ... | 2014 | 24709084 |
| characterization of transthyretin in the pacific bluefin tuna, thunnus orientalis. | a cdna encoding transthyretin was cloned from the pacific bluefin tuna (thunnus orientalis). this cdna contains a complete open reading frame encoding 151 amino acid residues. the deduced amino acid sequence is 81% and 55% identical to the gilthead seabream and common carp forms, respectively, and 33-39% to mammalian, reptilian, and amphibian forms. a 1.0-kb transcript was found in the the liver and ovary; the liver is the main source of this protein. analysis of triiodo-l-thyronine (t(3)) and l ... | 2006 | 16766863 |
| two types of tumor necrosis factor-alpha in bluefin tuna (thunnus orientalis) genes: molecular cloning and expression profile in response to several immunological stimulants. | tumor necrosis factor-alpha (tnf-alpha) is a key inflammatory mediator and has also the potential as a prominent biomarker of innate immunity. in this study, we identified and characterized tnf-alpha from bluefin tuna, which is an important cultured species. two types of tnf-alpha were also cloned incidentally (tnf1 and tnf2). the open reading frame of tnf1 and tnf2 cdna encoded 247 and 245 amino acids, respectively. the amino acid sequence identity among sea perch, red sea bream, and tiger puff ... | 2009 | 19146959 |
| amounts and compositional analysis of glycosaminoglycans in the tissue of fish. | we isolated gags from the inedible parts; head, skin, internal organs, fins, scales and spine, of atlantic mackerel (scomber scombrus), japanese jack mackerel (trachurus japonicus), pacific bluefin tuna (thunnus orientalis), yellowfin sole (limanda aspera), broadbanded thornyhead (sebastolobus macrochir), golden threadfin bream (nemipterus virgatus), and nile tilapia (oreochromis niloticus). we also investigated deep-sea fish, eelpouts (bothrocara hollandi, lycodes toyamensis, and lycodes nakamu ... | 2013 | 23261779 |