| formation of mutagens during the frying of hawaiian fish: correlation with creatine and creatinine content. | compounds mutagenic toward salmonella typhimurium strain ta98 in the presence of rat-liver homogenates (s9) were formed when fish flesh was fried at 199 degrees c. three species of hawaiian fish commonly consumed in hawaii (skipjack tuna, katsuwonus pelamis; yellowfin tuna, neothunnus macropterus; and milkfish, chanos chanos) were cooked in an electric skillet, along with samples of sole (microstomus pacificus). organic extracts of the fish were tested in the ames salmonella mutagenic assay usin ... | 1990 | 2270090 |
| comparative oxygen affinity of fish and mammalian myoglobins. | myoglobins from rat, coho salmon (oncorhynchus kisutch), buffalo sculpin (enophrys bison) hearts, and yellowfin tuna (thunnus albacares) red skeletal muscle were partially purified and their o2 binding affinities determined. commercially prepared sperm whale myoglobin was employed as an internal standard. tested at 20 degrees c, myoglobins from salmon and sculpin bound o2 with lower affinity than myoglobins from the rat or sperm whale. oxygen binding studies at 12 degrees c and 37 degrees c sugg ... | 1989 | 2760286 |
| oxygen sensitive afferent information arising from the first gill arch of yellowfin tuna. | single nerve fiber discharge was recorded from o2 sensitive receptors in the first gill arch of the yellowfin tuna, thunnus albacares, in vitro. these receptors were innervated by the vagus nerve and increased their discharge in response to decreasing perfusion rate, decreasing perfusion po2 and, in most fibers, to decreasing external po2. fibers responding to environmental hypoxia exhibited an exponential increase in discharge to decreasing external po2 with a sensitivity similar to that exhibi ... | 1986 | 3809755 |
| isolation and characterization of metmyoglobin reductase from yellowfin tuna (thunnus albacares). | the isolation, purification, and characterization of metmyoglobin reductase from yellowfin tuna (thunnus albacares) is described. the enzyme has been purified 120-fold. characterization of the enzyme includes molecular weight, isoelectric point, substrate specificity, enzyme kinetics, chromatographic behavior, and sensitivity to inhibitors. the physical and catalytic properties of the tuna enzyme are compared to those of bovine and blue-white dolphin metmyoglobin reductase. | 1985 | 4042625 |
| effects of sodium chloride concentration on solubility and electrophoretic characteristics of protein from the eye lens nucleus in a yellowfin tuna (thunnus albacares) and in a desert wood rat (neotoma lepida). | | 1968 | 5758384 |
| shark myoglobins i. isolation and characterization of myoglobins from the sharks, squalus japonicus and proscyllium habereri. | native oxymyoglobins from the sharks, squalus japonicus and proscyllium habereri were also isolated directly from red muscle. the essential step was the chromatographic separation of oxymyoglobin from metmyoglobin on a deae-cellulose column. the rate of autoxidation of native oxymyoglobin to metmyoglobin was examined over the ph range of 5-12 in 0.1 m buffer at 25 degrees c and the logarithms of the observed first-order rate constants, log (kobs), were plotted as a function of ph. the ph depende ... | 1984 | 6744819 |
| myoglobins of cartilaginous fishes. ii. isolation and amino acid sequence of myoglobin of the shark mustelus antarcticus. | myoglobin isolated from red muscle of the gummy shark m. antarcticus was purified by gel filtration and ion-exchange chromatography on carboxymethyl cellulose in 8 m urea-thiol buffer. amino acid analysis and sequence determination showed 148 amino acid residues. the amino terminal residue is acetylated as shown by nuclear magnetic resonance and mass spectrographic analysis of an n-terminal peptide. there is a deletion of four residues at the amino terminal end as well as one residue in the cd i ... | 1980 | 7436864 |
| characterization of yellowfin tuna (thunnus albacares, scombroidei) tryptophan hydroxylase. | tryptophan hydroxylase (ec 1.14, 16.4) was purified from yellowfin tuna liver and properties of this enzyme were compared with those of tryptophan hydroxylase from some other species (mouse mastocytoma and rat brain-stem). the molecular weight of the yellowfin tuna enzyme was estimated to be about 280,000 da. this value is similar to that for the enzymes from mouse mastocytoma and rat brain-stem. on sds-polyacrylamide gel electrophoresis analysis, yellowfin tuna enzyme was estimated to be about ... | 1997 | 9159879 |
| blood volume, plasma volume and circulation time in a high-energy-demand teleost, the yellowfin tuna (thunnus albacares) | we measured red cell space with 51cr-labeled red blood cells, and dextran space with 500 kda fluorescein-isothiocyanate-labeled dextran (fitc-dextran), in two groups of yellowfin tuna (thunnus albacares). red cell space was 13.8+/-0.7 ml kg-1 (mean +/- s.e.m.) assuming a whole-body hematocrit equal to the hematocrit measured at the ventral aortic sampling site and no significant sequestering of 51cr-labeled red blood cells by the spleen, blood volume was 46. 7+/-2.2 ml kg-1. this is within the r ... | 1998 | 9450974 |
| evolutionary and physiological variation in cardiac troponin c in relation to thermal strategies of fish. | striated muscle contraction is initiated when troponin c (tnc) binds ca(2+), which activates actinomyosin atpase. we investigated (i) the variation between cardiac tnc (ctnc) primary structure within teleost fish and (ii) the pattern of tnc expression in response to temperature acclimation. there were few differences between rainbow trout (oncorhynchus mykiss), yellowfin tuna (thunnus albacares), yellow perch (perca flavescens), goldfish (carassius auratus), white sucker (catostomus commersoni), ... | 2000 | 11121357 |
| a comparison of myocardial beta-adrenoreceptor density and ligand binding affinity among selected teleost fishes. | this study quantified the cell surface beta-adrenoreceptor density and ligand binding affinity in the ventricular tissue of seven teleost species; skipjack tuna (katsowonus pelamis), yellowfin tuna (thunnus albacares), pacific mackerel (scomber japonicus), mahimahi (dolphin fish; coryphaena hippurus), sockeye salmon (oncorhynchus nerka), rainbow trout (oncorhynchus mykiss) and an antarctic nototheniid (trematomus bernacchii). beta-adrenoreceptor density varied by almost fourfold among these spec ... | 2000 | 11128445 |
| comparative analysis of autoxidation of haemoglobin. | autoxidation of oxyhaemoglobin (oxyhb) to methaemoglobin was measured at different temperatures in haemoglobin solutions from atlantic hagfish, river lamprey, common carp, yellowfin tuna and pig. the aims were to evaluate the impact of the absent distal histidine in hagfish haemoglobin, the importance of oxyhb being either monomeric (hagfish and lamprey) or tetrameric (carp, tuna and pig) and to gain information on the temperature-sensitivity of autoxidation. the rate of autoxidation was lower i ... | 2001 | 11441044 |
| some digenea parasites of tunny from the coast of rio de janeiro state, brazil. | rhipidocotyle pentagonum (ozaki, 1924) is reported for the first time in south america parasitizing auxis thazard and in a new host katsuwonus pelamis. tergestia laticollis (rudolphi, 1819) is reported for the first time in south america and in thunnus albacares, representing a new host record. copiatestes filiferus (leuckart, in sars, 1885) is recorded for the first time in brazil and in thunnus albacares, another new host record. tetrochetus coryphaenae (yamaguti, 1934) is presented for the fi ... | 2002 | 12530181 |
| sources and variations of mercury in tuna. | while the bulk of human exposure to mercury is through the consumption of marine fish, most of what we know about mercury methylation and bioaccumulation is from studies of freshwaters. we know little of where and how mercury is methylated in the open oceans, and there is currently a debate whether methylmercury concentrations in marine fish have increased along with global anthropogenic mercury emissions. measurements of mercury concentrations in yellowfin tuna caught off hawaii in 1998 show no ... | 2003 | 14717163 |
| significant histamine formation in tuna (thunnus albacares) at 2 degrees c--effect of vacuum- and modified atmosphere-packaging on psychrotolerant bacteria. | occurrence and importance of psychrotolerant histamine producing bacteria in chilled fresh tuna were demonstrated in the present study. the objective was to evaluate microbial formation of histamine and biogenic amines in chilled fresh tuna from the indian ocean and stored either vacuum-packed (vp) or modified atmosphere-packed (map). firstly, biogenic amines and the dominating microbiota were determined in vp tuna involved in an outbreak of histamine fish poisoning in denmark. secondly, the mic ... | 2005 | 15925710 |
| circulating serotonin in vertebrates. | the role of circulating serotonin is unclear and whether or not serotonin is present in the blood of non-mammalian species is not known. this study provides the first evidence for the presence of serotonin in thrombocytes of birds and three reptilian species, the endothermic leatherback sea turtle, the green sea turtle and the partially endothermic american alligator. thrombocytes from a fresh water turtle, american bullfrog, yellowfin tuna, and chinook salmon did not contain serotonin. serotoni ... | 2005 | 16041566 |
| heavy metals in commercial fish in new jersey. | levels of contaminants in fish are of particular interest because of the potential risk to humans who consume them. while attention has focused on self-caught fish, most of the fish eaten by the american public comes from commercial sources. we sampled 11 types of fish and shellfish obtained from supermarkets and specialty fish markets in new jersey and analyzed them for arsenic, cadmium, chromium, lead, manganese, mercury, and selenium. we test the null hypothesis that metal levels do not vary ... | 2005 | 16307983 |
| multivalvulid myxozoans from eastern australia: three new species of kudoa from scombrid and labrid fishes of the great barrier reef, queensland, australia. | three new species of kudoa, each having 6 polar capsules, are described from the somatic muscle of fishes collected on the great barrier reef, queensland, australia. kudoa grammatorcyni n. sp. was observed in the shark mackerel grammatorcynus bicarinatus. spores are stellate in apical view, width (all measurements in microm) 8.62 (8.03-8.95); thickness 8.14 (7.63-8.68); suture width 7.7 (7.24-8.16); length 6.54 (6.32-6.71); polar capsule length 3.68 (3.55-3.82); polar capsule width 1.72 (1.65-1. ... | 2005 | 16419761 |
| mercury content in commercial pelagic fish and its risk assessment in the western indian ocean. | as top predators of pelagic food webs, large fish naturally bioaccumulate mercury (hg). determining hg burdens in commercialized fish is essential considering the concern about effects of contaminants on human health and the legal thresholds that are therefore set for local consumption and/or exportation. total hg levels were measured in the muscular tissue of 183 fish of five commercially important species from the tropical zone of the western indian ocean. all individuals were measured and sex ... | 2006 | 16580709 |
| detecting fish parvalbumin with commercial mouse monoclonal anti-frog parvalbumin igg. | parvalbumin is a calcium-binding muscle protein that is highly conserved across fish species and amphibians. it is the major cross-reactive allergen associated with both fish and frog allergy. we used two-dimensional electrophoretic and immunoblotting techniques to investigate the utility of a commercial monoclonal anti-frog parvalbumin igg for detecting parvalbumin present in some commonly consumed fish species. the 2d electrophoresis and immunoblots revealed species-specific differences in pro ... | 2006 | 16848548 |
| [detection of fish dna in ruminant feed by pcr amplification]. | the japanese government has prohibited the use of seafood protein, as well as mammalian protein, in ruminant feed. there is an official method to detect meat and bone meal, but no method is yet available to detect fishmeal in ruminant feed. we tried to develop a suitable method to detect fishmeal in ruminant feed, similar to the official method "pcr detection of animal-derived dna in feed". our previously reported primers (fishcon5 and fishcon3-1) showed low sensitivity, so we designed new prime ... | 2006 | 17128872 |
| effects of hypoxia on vertebrate blood vessels. | hypoxia contracts mammalian respiratory vessels and increases vascular resistance in respiratory tissues of many vertebrates. in systemic vessels these responses vary, hypoxia relaxes mammalian vessels and contracts systemic arteries from cyclostomes. it has been proposed that hypoxic vasoconstriction in cyclostome systemic arteries is the antecedent to mammalian hypoxic pulmonary vasoconstriction, however, phylogenetic characterization of hypoxic responses is lacking. in this study, we characte ... | 2008 | 18214862 |
| alaska pollack protein prevents the accumulation of visceral fat in rats fed a high fat diet. | in the first study (study 1), 4-wk-old sprague-dawley (sd) rats were fed high fat diets containing casein, alaska pollack, yellowfin tuna, or chicken as the protein source for 28 d. the purpose of this study was to compare the effect of alaska pollack protein with other animal proteins (casein, yellowfin tuna, and chicken) on the prevention of visceral fat accumulation. we found that alaska pollack protein was a more potent inhibitor of visceral fat accumulation than the other proteins (p<0.05). ... | 2009 | 19436142 |
| estimation of the carbon footprint of the galician fishing activity (nw spain). | the food production system as a whole is recognized as one of the major contributors to environmental impacts. accordingly, food production, processing, transport and consumption account for a relevant portion of the greenhouse gas (ghg) emissions associated with any country. in this context, there is an increasing market demand for climate-relevant information regarding the global warming impact of consumer food products throughout the supply chains. this article deals with the assessment of th ... | 2010 | 20800266 |
| detection and identification of histamine-producing bacteria associated with harvesting and processing mahimahi and yellowfin tuna. | histamine poisoning is one of the most common chemically induced seafoodborne illnesses reported in the united states today. the causative agents are biogenic amines, commonly produced by gram-negative bacteria. the purpose of this study was to detect and identify histamine-producing bacteria associated with standard industry practices during the harvesting, receiving, and processing of mahimahi and yellowfin tuna in north carolina. twenty-nine composite samples were obtained from 18 mahimahi an ... | 2005 | 21132978 |
| development of a real-time pcr assay with an internal amplification control for detection of gram-negative histamine-producing bacteria in fish. | prompt detection of bacteria that contribute to scombrotoxin (histamine) fish poisoning can aid in the detection of potentially toxic fish products and prevent the occurrence of illness. we report development of the first real-time pcr method for rapid detection of gram-negative histamine-producing bacteria (hpb) in fish. the real-time pcr assay was 100% inclusive for detecting high-histamine producing isolates and did not detect any of the low- or non-histamine producing isolates. the efficienc ... | 2010 | 21356438 |
| permanent genetic resources added to molecular ecology resources database 1 october 2010-30 november 2010. | this article documents the addition of 277 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: ascochyta rabiei, cambarellus chapalanus, chionodraco hamatus, coptis omeiensis, cynoscion nebulosus, daphnia magna, gerbillus nigeriae, isurus oxyrinchus, lates calcarifer, metacarcinus magister, oplegnathus fasciatus, pachycondyla verenae, phaethon lepturus, pimelodus grosskopfii, rotylenchulus reniformis, scomberomorus niphonius, sep ... | 2011 | 21429157 |
| bioaccumulation of mercury in muscle tissue of yellowfin tuna, thunnus albacares, of the eastern pacific ocean. | tuna, like most large pelagic fish, are highly exploited by man, and it is, therefore, important to determine mercury (hg) levels in these species in order to establish allowable limits for their consumption and/or contamination levels in the environment. in this study, we evaluated hg accumulation in yellowfin tuna (thunnus albacares) muscle in two different geographic sites of the eastern pacific ocean. there was a positive association between hg content and tuna size in the equatorial zone (e ... | 2011 | 21739161 |
| toxic metals in commercial marine fish in oman with reference to national and international standards. | commercially important fresh (581) and frozen (292) marine fish samples of 10 species were collected from seafood factories and evaluated using aas and icp-oes. metal levels significantly (p<0.05) varied within and between species. however, there were no significant correlations among metals. there were significant interspecific differences for all metals, and yellowfin tuna had the highest level of cadmium and mercury however, red seabream had maximum numbers above the standards. the metal accu ... | 2011 | 21700309 |
| quantification of total and specific gram-negative histamine-producing bacteria species in fish using an mpn real-time pcr method. | quantification of histamine-producing bacteria (hpb) is necessary in order to elucidate the role that hpb play in scombrotoxin (histamine) fish poisoning. we report here the evaluation of a real-time pcr method for the quantification of total and specific gram-negative hpb species in fish using a most probable number (mpn) format. the species-specific real-time pcr assay was 100% inclusive for independently detecting morganella morganii, enterobacter aerogenes, raoultella planticola/ornithinolyt ... | 2011 | 21839377 |
| Total mercury in canned yellowfin tuna Thunnus albacares marketed in northwest Mexico. | Mercury (Hg) was determined in Thunnus albacares canned in oil (from 7 to 10 samples per brand) and water (from 5 to 10 samples per brand) of five leading brands in Mexico in 2008. Potential health risk was estimated on the basis of Hg concentration and rate (1.43 kg year(-1)per capita) of tuna consumption in Mexico. Highest Hg concentrations were 0.51 ± 0.26 and 0.40 ± 0.24 µ gg(-1) dry weight in water and oil, respectively. Averaged Hg concentrations in tuna canned in water in the current stud ... | 2011 | 21911030 |
| pcr detection and identification of histamine-forming bacteria in filleted tuna fish samples. | total of 14 filleted yellowfin tuna fish (thunnus albacares) sold in wholesale fish market and supermarkets in milan, italy, were purchased and tested to determine microbial count, histamine level, histamine-forming bacteria, and their ability to produce histamine in culture broth. although histamine level was less than 10 ppm, many samples showed high total viable bacterial and enterobacterial counts that reached dangerous levels after temperature abuse for short periods of time. a pcr assay ta ... | 2012 | 22251187 |
| antimicrobial function of the gapdh-related antimicrobial peptide in the skin of skipjack tuna, katsuwonus pelamis. | a 3.4 kda of antimicrobial peptide was purified from an acidified skin extract of skipjack tuna, katsuwonus pelamis, by preparative acid-urea-polyacrylamide gel electrophoresis and c18 reversed-phase hplc. a comparison of the n-terminal amino acid sequence of the purified peptide with that of other known polypeptides revealed high sequence homology with the yfgap (yellowfin tuna glyceraldehyde-3-phosphate dehydrogenase-related antimicrobial peptide); thus, this peptide was identified as the skip ... | 2014 | 24412436 |
| purification and characterization of yfgap, a gapdh-related novel antimicrobial peptide, from the skin of yellowfin tuna, thunnus albacares. | a 3.4 kda of antimicrobial peptide was purified from an acidified skin extract of the yellowfin tuna, thunnus albacares, by preparative acid-urea-polyacrylamide gel electrophoresis and c(18) reversed-phase hplc. a comparison of the n-terminal amino acid sequence of the purified peptide with that of other known polypeptides revealed high homology with the n-terminus of glyceraldehyde-3-phosphate dehydrogenase (gapdh); thus, this peptide was designated as the yellowfin tuna gapdh-related antimicro ... | 2012 | 22771964 |
| antigenicity of anisakis simplex s.s. l3 in parasitized fish after heating conditions used in the canning processing. | some technological and food processing treatments applied to parasitized fish kill the anisakis larvae and prevent infection and sensitization of consumers. however, residual allergenic activity of parasite allergens has been shown. the aim here was to study the effect of different heat treatments used in the fish canning processing industry on the antigen recognition of anisakis l3. bigeye tuna (thunnus obesus) and yellowfin tuna (thunnus albacares) were experimentally infected with live l3 ani ... | 2015 | 24888867 |
| identification of anisakis species (nematoda: anisakidae) in marine fish hosts from papua new guinea. | the third-stage larvae of several genera of anisakid nematodes are important etiological agents for zoonotic human anisakiasis. the present study investigated the prevalence of potentially zoonotic anisakid larvae in fish collected on the coastal shelves off madang and rabaul in papua new guinea (png) where fish represents a major component of the diet. nematodes were found in seven fish species including decapterus macarellus, gerres oblongus, pinjalo lewisi, pinjalo pinjalo, selar crumenophtha ... | 2013 | 23290280 |
| importance of histamine-producing clostridium perfringens in scombrotoxin-forming fish. | it has been suggested that anaerobic histamine-producing bacteria (hpb) are important contributors to scombrotoxin fish poisoning (sfp). in order to assess the role of clostridium perfringens in sfp, we developed a real-time pcr method for rapid detection of histamine-producing (hp) c. perfringens. the real-time pcr assay was 100% inclusive for detecting 23 hp c. perfringens and did not detect any of the other 116 hp or non-hp isolates examined. the efficiency of the assay with or without intern ... | 2013 | 23834808 |
| biogenic amine content, histamine-forming bacteria, and adulteration of pork in tuna sausage products. | twenty-five tuna sausage products were purchased from retail markets in taiwan. the rates of occurrence of biogenic amines, histamine-forming bacteria, and adulteration by pork and poultry were determined. the average content of various biogenic amines in all tested samples was less than 2.0 mg/100 g (<0.05 to 1.85 mg/100 g). thirteen histamine-producing bacterial strains isolated from tested samples produced 12.1 to 1,261 ppm of histamine in trypticase soy broth supplemented with 1.0% l-histidi ... | 2012 | 23043830 |
| behavior of salmonella and listeria monocytogenes in raw yellowfin tuna during cold storage. | behavior of salmonella and listeria monocytogenes in raw yellowfin tuna during refrigeration and frozen storage were studied. growth of salmonella was inhibited in tuna during refrigerated storage, while l. monocytogenes was able to multiply significantly during refrigerated storage. populations of salmonella in tuna were reduced by 1 to 2 log after 12 days of storage at 5-7 °c, regardless levels of contamination. however, populations of l. monocytogenes scott a, m0507, and sfl0404 in inoculated ... | 2016 | 28231111 |
| skin lesions on yellowfin tuna thunnus albacares from gulf of mexico outer continental shelf: morphological, molecular, and histological diagnosis of infection by a capsalid monogenoid. | we characterize lesion-associated capsaline infections on yellowfin tuna, thunnus albacares, in the gulf of mexico by comparing our specimens with published descriptions and museum specimens ascribed to capsala biparasiticum and its synonyms: vouchers of c. biparasiticum from parasitic copepods; the holotype of capsala neothunni; and vouchers of capsala abidjani. those from parasitic copepods differed by having a small, rounded body, large anterior attachment organs, closely spaced dorsomarginal ... | 2015 | 26277272 |
| molecules infer origins of ectoparasite infrapopulations on tuna. | infrapopulation genetic variation of the oioxenous, hermaphroditic flatworm nasicola klawei (monogenea: capsalidae) infecting the nasal cavities of nine yellowfin tuna, thunnus albacares, from the gulf of mexico was analyzed using the first internal transcribed spacer (its1) single strand conformation polymorphism (sscp), its1 sequencing, and amplified fragment length polymorphism (aflp). of a total of 32 worms, six had unique its1-sscp types and the rest was grouped by three types. two worms of ... | 2011 | 21840416 |
| the oncomiracidium of nasicola klawei (monogenea: capasalidae: capsalinae). | the oncomiracidium of the capsaline nasicola klawei (monogenea: capsalidae), adults of which were collected from the nares of the yellowfin tuna thunnus albacares (scombridae) caught off nelson bay on the east coast of australia, is described. this is a new locality record for n. klawei. this is the first description of an oncomiracidium of a nasicola species and only the third time the larva of a capsaline species has been documented. the oncomiracidium of n. klawei is distinguished from others ... | 2007 | 16941186 |
| draft genome sequences of histamine-producing photobacterium kishitanii and photobacterium angustum, isolated from albacore (thunnus alalunga) and yellowfin (thunnus albacares) tuna. | histamine-producing bacteria are responsible for scombrotoxin (histamine) fish poisoning, a leading cause of fish poisoning in the united states. we report here the draft genome sequences of four histamine-producing (hp) photobacterium kishitanii strains and nine hp photobacterium angustum strains isolated from tuna. | 2015 | 25931609 |
| preparation and structural analysis of actinidain-processed atelocollagen of yellowfin tuna (thunnus albacares). | pepsin-hydrolyzed collagen (atelocollagen) is a trimer, consisting of alpha 1 and alpha 2 monomers, and shows molecular species corresponding to a monomer, dimer (beta chain), and trimer (gamma chain) by sds-polyacrylamide gel electrophoresis. atelocollagen was purified from yellowfin tuna (thunnus albacares) by salt precipitation and cation-exchange chromatography. enzymatic hydrolysis of the atelocollagen by actinidain, a cysteine protease purified from kiwifruit, was analyzed by sds-polyacryl ... | 2004 | 15118315 |
| slow muscle function of pacific bonito (sarda chiliensis) during steady swimming. | the pacific bonito, sarda chiliensis, is anatomically intermediate between mackerel and tuna. the specialisations exhibited by tuna are present in the bonito, but to a lesser degree. slow-twitch muscle strain and activity patterns were determined during steady swimming (tailbeat frequency 1.2-3.2 hz) at four locations on the body of sarda chiliensis using sonomicrometry and electromyography. both strain and the phase of electromygraphic activity were independent of tailbeat frequency. the strain ... | 2000 | 10851117 |
| the differentiation of tuna (family: scombridae) products through the pcr-based analysis of the cytochrome b gene and parvalbumin introns. | in spite of the many studies performed over the years, there are still problems in the authentication of closely related tuna species, not only for canned fish but also for raw products. with the aim of providing screening methods to identify different tuna species and related scombrids, segments of mitochondrial cytochrome b (cyt b) and nuclear parvalbumin genes were amplified and sequenced or subjected to single-strand conformation polymorphism (sscp) and restriction fragment length polymorphi ... | 2016 | 25639273 |
| mitochondrial dna variation and phylogenetic relationships among five tuna species based on sequencing of d-loop region. | in order to assess the dna sequence variation and phylogenetic relationship among five tuna species (auxis thazard, euthynnus affinis, katsuwonus pelamis, thunnus tonggol, and t. albacares) out of all four tuna genera, partial sequences of the mitochondrial dna (mtdna) d-loop region were analyzed. the estimate of intra-specific sequence variation in studied species was low, ranging from 0.027 to 0.080 [kimura's two parameter distance (k2p)], whereas values of inter-specific variation ranged from ... | 2016 | 25329285 |
| total mercury and methylmercury accumulation in the muscle tissue of frigate (auxis thazard thazard) and yellow fin (thunnus albacares) tuna from the gulf of guinea, ghana. | | 2006 | 16786455 |
| tunas as small as 207 mm fork length can elevate muscle temperatures significantly above ambient water temperature | tunas (family scombridae) maintain muscle temperatures (tm) elevated significantly above ambient water temperature (ta) by using vascular counter-current heat exchangers (retia mirabilia) to conserve metabolic heat generated by continuous contraction of red muscle fibers. previous work has documented endothermy and both behavioral and physiological thermoregulation in tunas, but only individuals greater than approximately 1 kg body mass had been studied. the purpose of this study was to measure ... | 1994 | 9317376 |
| thermal characterisation of gelatin extracted from yellowfin tuna skin and commercial mammalian gelatin. | glass transition and other thermal characteristics of gelatin from different sources were studied by differential scanning calorimetry (dsc) and modulated dsc (mdsc). the initial glass transition temperatures of equilibrated gelatin samples at 11.3% relative humidity, determined from reversible heat flow thermogram of mdsc, were 23, 75 and 59°c, respectively, for tuna skin, bovine and porcine gelatin. when gelatin samples were equilibrated at higher relative humidity of 52.9%, glass transition t ... | 2008 | 26059124 |
| [reasons of drifting floating objects aggregating rainbow runner (elagatis bipinnulata)]. | many pelagic species tend to aggregate under drifting floating objects. this has led to the development of drifting fish aggregation devices (fads) to attract the tropical tunas for the tuna purse seine fishery. however, fads can also attract other non-targeting small pelagic species such as rainbow runner elagatis bipinnulata, although it is still unclear why those species can be attracted and aggregated under an fad. using the fishery biological data collected in the western and central pacifi ... | 2014 | 24765868 |
| isolation of heat-tolerant myoglobin from asian swamp eel monopterus albus. | myoglobin from asian swamp eel monopterus albus was purified from fish muscle using salt fractionation followed by column chromatography and molecular filtration. the purified mb of 0.68 mg/g wet weight of muscle was determined for its molecular mass by maldi-tof-ms to be 15,525.18 da. using isoelectric focusing technique, the purified mb showed two derivatives with pi of 6.40 and 7.12. six peptide fragments of this protein identified by lc-ms/ms were homologous to mbs of sea raven hemitripterus ... | 2012 | 22538454 |
| myoglobin interspecies structural differences: effects on autoxidation and oxygenation. | thermodynamic and kinetic parameters of the oxygenation and autoxidation reactions were determined for yellowfin tuna (thunnus albacares), pacific green sea turtle (chelonia mydas caranigra), and sperm whale myoglobin. these proteins have quite similar heme contact residues, but vary in residues which are involved in globin stabilization and in postulated ligand paths to the heme iron. oxygen equilibria measurements from 10 to 40 degrees c reveal that green sea turtle myoglobin has a lower oxyge ... | 1986 | 3740846 |
| a novel system for embryo-larval toxicity testing of pelagic fish: applications for impact assessment of deepwater horizon crude oil. | key differences in the developmental process of pelagic fish embryos, in comparison to embryos of standard test fish species, present challenges to obtaining sufficient control survival needed to successfully perform traditional toxicity testing bioassays. many of these challenges relate to the change in buoyancy, from positive to negative, of pelagic fish embryos that occurs just prior to hatch. a novel exposure system, the pelagic embryo-larval exposure chamber (pelec), has been developed to c ... | 2016 | 27505137 |
| heavy metals in yellowfin tuna (thunnus albacares) and common dolphinfish (coryphaena hippurus) landed on the ecuadorian coast. | heavy metals are contaminants of great environmental concern due to their multiple origins (natural and anthropogenic), the ability to accumulate in organs and tissues, and the deleterious effects they can cause in organisms. studies on the accumulation of metals in seafood, such as fish, have increased in importance due to the risk for human health when consuming fish contaminated by metals. the present work was aimed at verifying the concentrations of cadmium (cd), mercury (hg) and lead (pb) i ... | 2016 | 26406109 |
| trophic influences on mercury accumulation in top pelagic predators from offshore new england waters of the northwest atlantic ocean. | trophic pathways and size-based bioaccumulation rates of total mercury were evaluated among recreationally caught albacore tuna (thunnus alalunga), yellowfin tuna (thunnus albacares), shortfin mako shark (isurus oxyrinchus), thresher shark (alopias vulpinus), and dolphinfish (coryphaena hippurus) from offshore southern new england waters of the northwest atlantic ocean between 2008 and 2011. mercury concentrations were highest in mako (2.65 ± 1.16 ppm) and thresher sharks (0.87 ± 0.71 ppm), and ... | 2014 | 25440782 |
| consistently low mercury concentrations in dolphinfish, coryphaena hippurus, an oceanic pelagic predator. | compared to total mercury concentrations in other oceanic pelagic and syntopic fish species examined from waters of the southeastern united states (e.g., king mackerel, scomberomorus cavalla; wahoo, acanthocybium solanderi; yellowfin tuna, thunnus albacares; blackfin tuna, t. atlanticus), total mercury concentrations in dolphinfish, coryphaena hippurus, examined were consistently low. dorsal-muscle tissue from 385 dolphinfish (317-1395 mm fork length) from florida offshore waters was analyzed fo ... | 2009 | 19539279 |
| temperature sensitivity of cardiac function in pelagic fishes with different vertical mobilities: yellowfin tuna (thunnus albacares), bigeye tuna (thunnus obesus), mahimahi (coryphaena hippurus), and swordfish (xiphias gladius). | we measured the temperature sensitivity, adrenergic sensitivity, and dependence on sarcoplasmic reticulum (sr) ca(2+) of ventricular muscle from pelagic fishes with different vertical mobility patterns: bigeye tuna (thunnus obesus), yellowfin tuna (thunnus albacares), and mahimahi (coryphaena hippurus) and a single specimen from swordfish (xiphias gladius). ventricular muscle from the bigeye tuna and mahimahi exhibited a biphasic response to an acute decrease in temperature (from 26 degrees to 7 ... | 2013 | 19284308 |
| effects of on-board and dockside handling on the formation of biogenic amines in mahimahi (coryphaena hippurus), skipjack tuna (katsuwonus pelamis), and yellowfin tuna (thunnus albacares). | consumer illnesses by scombroid poisonings have been a continuing problem for many years. the intoxications follow the ingestion of fish such as tuna and mahimahi that have undergone bacterial decomposition, leading to the formation of biogenic amines. research studies have concluded that histamine is one of the indicators of scombrotoxic fish and that other amines, such as cadaverine, could be involved in the illnesses. guidance for the handling of fish on board fishing vessels to prevent the p ... | 2004 | 14717363 |
| hydrogen ion binding properties of tuna haemoglobins. | tunas are very active fish with a high aerobic capacity, but they also regularly perform burst swimming with massive production of lactic acid. the present study examines whether h(+) buffering by tuna haemoglobin (hb) is elevated to cope with metabolic acidoses (by analogy with the high buffer capacity of tuna white musculature) or whether the hb-h(+) binding properties resemble other teleosts that have low buffer values and high haldane effects. h(+) titration of oxygenated and deoxygenated co ... | 2001 | 11423321 |
| predicting interactions between common dolphins and the pole-and-line tuna fishery in the azores. | common dolphins (delphinus delphis) are responsible for the large majority of interactions with the pole-and-line tuna fishery in the azores but the underlying drivers remain poorly understood. in this study we investigate the influence of various environmental and fisheries-related factors in promoting the interaction of common dolphins with this fishery and estimate the resultant catch losses. we analysed 15 years of fishery and cetacean interaction data (1998-2012) collected by observers plac ... | 2016 | 27851763 |
| hearing thresholds of swimming pacific bluefin tuna thunnus orientalis. | pacific bluefin tuna (thunnus orientalis) is a highly migratory, commercially valuable species potentially vulnerable to acoustic noise generated from human activities which could impact behavior and fitness. although significant efforts have been made to understand hearing abilities of fishes, the large size and need to continuously swim for respiration have hindered investigations with tuna and other large pelagic species. in this study, pacific bluefin tuna were trained to respond to a pure t ... | 2015 | 25732931 |
| swimming performance studies on the eastern pacific bonito sarda chiliensis, a close relative of the tunas (family scombridae) ii. kinematics. | the swimming kinematics of the eastern pacific bonito sarda chiliensis at a range of sustained speeds were analyzed to test the hypothesis that the bonito's swimming mode differs from the thunniform locomotor mode of tunas. eight bonito (fork length fl 47.5+/-2.1 cm, mass 1.25+/-0.15 kg) (mean +/- s.d.) swam at speeds of 50-130 cm s(-1) at 18+/-2 degrees c in the same temperature-controlled water tunnel that was used in previous studies of tunas. kinematics variables, quantified from 60 hz video ... | 2003 | 12847120 |
| studies of tropical tuna swimming performance in a large water tunnel - energetics | the metabolic rates (v(dot)o2) of three tropical tunas [yellowfin tuna (thunnus albacares), kawakawa (euthynnus affinis) and skipjack (katsuwonus pelamis)] were estimated using a large water-tunnel respirometer. experiments lasting up to 31 h were used to determine the effects of velocity (u) on tuna v(dot)o2 over a range of u (17-150 cm s-1) and temperatures (18­30°c). replicate tests were carried out on several fish. the swimming v(dot)o2 of yellowfin is temperature-dependent (q10=1.67 ... | 1994 | 9317243 |
| mercury in tunas and blue marlin in the north pacific ocean. | models and data from the north pacific ocean indicate that mercury concentrations in water and biota are increasing in response to (global or hemispheric) anthropogenic mercury releases. in the present study, we provide an updated record of mercury in yellowfin tuna (thunnus albacares) caught near hawaii that confirms an earlier conclusion that mercury concentrations in these fish are increasing at a rate similar to that observed in waters shallower than 1000 m. we also compiled and reanalyzed d ... | 2017 | 28264147 |
| responses of the red blood cells from two high-energy-demand teleosts, yellowfin tuna (thunnus albacares) and skipjack tuna (katsuwonus pelamis), to catecholamines. | in fishes, catecholamines increase red blood cell intracellular ph through stimulation of a sodium/proton (na+/h+) antiporter. this response can counteract potential reductions in blood o2 carrying capacity (due to bohr and root effects) when plasma ph and intracellular ph decrease during hypoxia, hypercapnia, or following exhaustive exercise. tuna physiology and behavior dictate exceptionally high rates of o2 delivery to the tissues often under adverse conditions, but especially during recovery ... | 1998 | 9747521 |
| total mercury, cadmium and lead levels in main export fish of sri lanka. | total mercury (hg), cadmium (cd) and lead (pb) levels were determined in the muscle of four commercialised exported fish species thunnus albacares (yellowfin tuna), xiphias gladius (swordfish), makaira indica (black marlin) and lutjanus sp (red snapper) collected from the indian ocean, sri lanka, during july 2009-march 2010 and measured by atomic absorption spectrophotometry. results show that swordfish (n = 176) contained the highest total hg (0.90 ± 0.51 mg/kg) and cd (0.09 ± 0.13 mg/kg) level ... | 2014 | 25070289 |
| kudoa hexapunctata n. sp. (myxozoa: multivalvulida) from the somatic muscle of pacific bluefin tuna thunnus orientalis and re-description of k. neothunni in yellowfin tuna t. albacares. | since kudoa septempunctata in olive flounder (paralichthys olivaceus) was indicated to cause food poisoning in humans, other kudoa species are suspected to have pathogenic potential. recently, a myxosporean possibly associated with food poisoning in humans consuming raw pacific bluefin tuna, thunnus orientalis, was identified as kudoa neothunni. this is a known causative myxosporean of post-harvest myoliquefaction in yellowfin tuna thunnus albacares. regardless of the significant differences in ... | 2014 | 24709084 |
| hirudinella ventricosa (pallas, 1774) baird, 1853 represents a species complex based on ribosomal dna. | digeneans in the genus hirudinella de blainville, 1828 (hirudinellidae) from three species of pelagic fishes, acanthocybium solandri (cuvier), makaira nigricans lacépède and thunnus albacares (bonnaterre), and one benthic fish, mulloidichthys martinicus (cuvier), from the gulf of mexico are investigated using comparison of ribosomal dna. four species are identified based on molecular differences: hirudinella ventricosa (pallas, 1774) baird, 1853 from a. solandri, hirudinella ahi yamaguti, 1970 f ... | 2013 | 24048751 |
| characterization of the ribosomal rna gene of kudoa neothunni (myxosporea: multivalvulida) in tunas (thunnus spp.) and kudoa scomberi n. sp. in a chub mackerel (scomber japonicus). | kudoa neothunni is the first described kudoa species having six shell valves and polar capsules, previously assigned to the genus hexacapsula arai and matsumoto, 1953. since its genetic analyses remain to be conducted, the present study characterizes the ribosomal rna gene (rdna) using two isolates from a yellowfin tuna (thunnus albacares) with post-harvest myoliquefaction and a northern bluefin tuna (thunnus thynnus) without tissue degradation. spores of the two isolates localized in the myofib ... | 2013 | 23455941 |