[cholinergic and adrenergic innervation of the arteries of the base of the brain in certain lower vertebrates].the arteries in the encephalon base have been investigated by koelle's and falck's methods in teleostei (anarhichas lupus, eleginus navaga, leuciscus brandti, pelingas), amphibians (rana temporaria, rana semiplicata, bufo bufo) and in reptiles (testudo horsfieldi, trionyx chinensis). cholin- and adrenergic nerve fibres of the encephalic arteries have been revealed in all the vertebrates studied, but their concentration is far from being equal. in fishes and reptiles neural conductors from scarse ...1979116627
wolffish antifreeze protein from transgenic drosophila.we have expressed two antifreeze protein genes from the atlantic wolffish, anarhichas lupus, in drosophila melanogaster by placing them under the divergent transcriptional control of the host yolk polypeptide (1 and 2) gene promoters. both genes were joined to the central promoter region by fusion within the dna encoding the signal polypeptides. the chimeric genes were introduced into flightless mutant drosophila through p-element transformation. transformed adult females from individual lines a ...19901367435
evaluation of pathogenic fungi occurrence in traumatogenic structures of freshwater fish.fungal infections in human skin, such as sporotrichosis, can occur after fish induced trauma. this work aimed to identify fungi in freshwater fish that are pathogenic to humans.201121468475
a fish bradykinin (arg0, trp5, leu8-bradykinin) from the defensive skin secretion of the european edible frog, pelophylax kl. esculentus: structural characterization; molecular cloning of skin kininogen cdna and pharmacological effects on mammalian smooth muscle.extensive studies on bradykinin-related peptides (brps) generated from plasma kininogens in representative species of various vertebrate taxa, have confirmed that many amphibian skin brps reflect those present in putative vertebrate predators. for example, the (val(1), thr(6))-bradykinin, present in the defensive skin secretions of many ranids and phyllomedusines, can be generated from plasma kininogens in colubrid snakes-common predators of these frogs. here, we report the presence of (arg(0), ...201020923691
historical influences dominate the population genetic structure of a sedentary marine fish, atlantic wolffish (anarhichas lupus), across the north atlantic ocean.abstract genetic variation was assessed in atlantic wolffish, anarhichas lupus, across the north atlantic ocean using microsatellite and amplified fragment length polymorphism (aflp) markers. despite unusual life history attributes such as large benthic eggs, large larvae, a limited pelagic stage and relatively sedentary adults, which suggest potential for strong population structure, range-wide f(st) values were comparable to other marine fishes (</=0.035). nevertheless, both significant geneti ...201020819162
phylogeography of 3 north atlantic wolffish species (anarhichas spp.) with phylogenetic relationships within the family anarhichadidae.phylogenetic analyses of all 4 wolffish species (atlantic, spotted, northern, and bering wolffishes) and the wolfeel were assessed with both mitochondrial (d-loop and nd1) and nuclear (amplified fragment length polymorphism) dna to resolve relationships within the family anarhichadidae. species-specific mitochondrial dna (mtdna) mutation rates were estimated based on 2 possible dates of divergence between the pacific and atlantic lineages. phylogeographic patterns within each of the 3 north atla ...201020511379
cathepsin d from atlantic cod (gadus morhua l.) liver. isolation and comparative studies.the isolated cathepsin d-like enzyme from atlantic cod (gadus morhua l.) liver was shown to be a monomer with a molecular mass of approximately 40 kda. it was inhibited by pepstatin a and had an optimum for degradation of haemoglobin at ph 3.0. the purified enzyme had lower temperature stability than bovine cathepsin d. antibodies raised against the purified enzyme and against two c-terminal peptides of cod cathepsin d recognized a 40 kda protein in immunoblotting of the samples from the purific ...200717428719
the effects of different predator species on antipredator behavior in the trinidadian guppy, poecilia reticulata.different types of predators often elicit different antipredator responses in a common type of prey. alternatively, some prey species may adopt a general response, which provides limited protection from many different types of predator. the trinidadian guppy, poecilia reticulata, is faced with a wide range of different predators throughout its range and is known to display varying levels of antipredator behavior depending on the predator assemblage. pike cichlids, crenicichla frenata, are regard ...200616896976
new method for rapid and sensitive quantification of sulphide-producing bacteria in fish from arctic and temperate waters.the offensive, fishy, rotten h2s-off-odours in spoiled, aerobically and cold stored fish from arctic and temperate waters are generally caused by sulphide-producing bacteria (spb), mainly shewanella putrefaciens. in the present work, a new, rapid, simple and accurate method for estimation of the spb content in fish from these areas is described. the quantification is based on the formation rate of iron sulphide during growth of spbs incubated at 30 degrees c in a liquid growth medium containing ...200415163589
epidemiology of bacterial diseases in norwegian aquaculture--a description based on antibiotic prescription data for the ten-year period 1991 to norway, antibacterial drugs for use in farmed fishes have to be prescribed by a veterinarian. moreover, a national surveillance programme requires that copies of all prescriptions be sent to the directorate of fisheries. the prescriptions give information regarding fish farm and locality, weight and species of fish to be medicated, diagnosis, type and amount of drug prescribed, and date. these prescription data for the 10 yr period 1991 to 2000 have been recorded and systematised. a total of ...200312650244
antifreeze protein gene amplification facilitated niche exploitation and speciation in wolffish.during winter, the coastal waters of newfoundland can be considered a 'freeze risk ecozone' for teleost fishes, where the shallower habitats pose a high (and the deeper habitats a low) risk of freezing. atlantic (anarhichas lupus) and spotted (anarhichas minor) wolffish, which inhabit these waters, reside at opposite ends of this ecozone, with the atlantic wolffish being the species facing the greatest risk, because of its shallower niche. in order to resist freezing, this species secretes five ...201222520964
cloning, sequence and variability analysis of expressed immunoglobulin light chain genes from yellowtail seriola quinqueradiata.the cdna clones encoding immunoglobulin (ig) light (l) chain variable (v) region associated with constant (c) region were isolated from yellowtail (seriola quinqueradiata) kidney by expressed sequence tag analysis (accession numbers: ab062619-ab062668, ab064322). the sequences of both vl and cl region contain well-conserved cysteine residues important for intra- and inter-domain interaction in mammals. comparisons of the amino acid sequence of the cl domain with those of other species showed a h ...200312547626
identification and characterization of carnobacteria isolated from fish intestine.eleven bacterial strains were isolated from the gastrointestinal tract of four fish species, atlantic salmon (salmo salar l.), arctic charr (salvelinus alpinus l.), atlantic cod (gadus morhua l.) and wolffish (anarhichas lupus l.). all the strains were gram-positive rods, non-sporing, catalase and oxidase-negative, able to grow at ph 9.0 but not on acetate containing media (ph < or = 5.4), and were fermentative. they had a high content of oleic acid (18:1 n-9) in cellular lipid, and were found t ...200111518321
an outbreak of cataract with lens rupture and nuclear extrusion in wolf-fish (anarhicas spp.).eight spotted (anarhicas minor olafsen) and five common wolf-fish (anarhicas lupus l), developed cataracts shortly after an episode of increased water temperature and decreased salinity 5 years prior to examination. on clinical examination, the cataracts were mostly bilateral and complete, and a majority of the lenses were lobulated. inflammatory reaction was, apart from one eye with severe inflammation, limited to iris atrophy. of the 14 eyes collected for pathomorphological examination, eight ...199811397203
increased gene dosage augments antifreeze protein levels in transgenic drosophila of the principal environmental adaptations of certain fishes inhabiting polar and northern coastal waters is the synthesis of antifreeze proteins (afps). afps bind to and prevent the growth of nascent ice crystals, thus depressing the serum freezing point. the transgenic expression of afp holds great promise for conferring freeze resistance to commercially important plant and animal species. since fish at the greatest risk of freezing have multiple afp gene copies in order to synthesize high ...199910399366
a comparative study of pentraxin-like proteins in different fish species.pentraxins are a family of pentameric serum proteins that have been conserved in evolution and share sequence homology, similar subunit assembly and the capacity for calcium-dependent ligand binding. the classical pentraxins are human c-reactive protein (crp) and serum amyloid p component (sap). the sequence homology and gene organization indicate that they arose from a gene duplication of an ancestral pentraxin gene. they are usually isolated based on their affinity for phosphorylcholine and ag ...19989639088
wolffish antifreeze protein genes are primarily organized as tandem repeats that each contain two genes in inverted orientation.the antifreeze protein genes of the wolffish (anarhichas lupus) constitute a large multigene family of 80 to 85 copies, which can be classified into two sets. one-third of the genes were linked but irregularly spaced. the other two-thirds were organized as 8-kilobase-pair (kbp) tandem direct repeats that each contained two genes in inverted orientation; dna sequence analysis suggests that both genes are functional. except for a single region specific to each gene, the genes and their immediate f ...19882851724
electrophoretic study of myosin isoforms in white muscles of some teleost fishes.1. white skeletal muscle myosin of four marine teleost fish species (cod, blue whiting, norway haddock, and spotted wolf-fish) was analyzed by native, sds-page, and 2-dimensional electrophoresis. 2. four types of native myosin were present in cod, blue whiting and norway haddock. the second fastest migrating form was predominant. 3. myosin from spotted wolf-fish also resolved into four forms. the fastest migrating form was hardly noticeable. the other three were present in apparently similar amo ...19902361358
[the origin of the locomotor and trophic activities in the young wolf-fish dicentrarchus labrax (l.) (pisces perranidae)].the spontaneous activities of a fixed type shown by the larvae of d. l. are at the beginning random (at least apparently) and not directed. the rheotactism which appears as soon as the eyes are pigmented has been used for the presentation of lures, thus allowing the study of the stimuli releasing the feeding activity and the breeding of 913 individuals up to the alevin stage.1975808350
[arterial monoaminocytes of the base of the brain in vertebrates].by falck's method monoaminocytes of the arteries in the encephalon base were studied in teleostei (anarhichas lupus, eleginus navaga, leuciscus brandti liza soiuy in amphibians (rana temporaria, rana semiplicata, bufo bufo), in reptiles (testudo horsfiedli, trionyx chinensis) and in birds (blue rock pigeon, hen). specific and individual peculiarities in chromaffinocyte, labrocyte and melanophore distribution were revealed in the vertebrate cranial arteries. in all the animals studied chromaffin ...1979518336
structure and ultrastructure of intra-vitam parasitic destruction of the external dental tissue in the fish, anarhichas lupus l. 1979299140
Whole chromosome painting reveals independent origin of sex chromosomes in closely related forms of a fish species.The wolf fish Hoplias malabaricus includes well differentiated sex systems (XY and X(1)X(2)Y in karyomorphs B and D, respectively), a nascent XY pair (karyomorph C) and not recognized sex chromosomes (karyomorph A). We performed the evolutionary analysis of these sex chromosomes, using two X chromosome-specific probes derived by microdissection from the XY and X(1)X(2)Y sex systems. A putative-sex pair in karyomorph A was identified, from which the differentiated XY system was evolved, as well a ...201121927842
the chromosomal distribution of microsatellite repeats in the genome of the wolf fish hoplias malabaricus, focusing on the sex chromosomes.distribution of 12 mono-, di- and tri-nucleotide microsatellites on the chromosomes of 2 karyomorphs with 2 distinct sex chromosome systems (a simple xx/xy - karyomorph b and a multiple x(1)x(1)x(2)x(2)/x(1)x(2)y - karyomorph d) in hoplias malabaricus, commonly referred to as wolf fish, was studied using their physical mapping with fluorescence in situ hybridization (fish). the distribution patterns of different microsatellites along the chromosomes varied considerably. strong hybridization sign ...201021099206
development of microsatellite markers for hoplias malabaricus (erythrinidae).we identified 14 microsatellite loci for the wolf fish, hoplias malabaricus (erythrinidae), from a genomic shotgun library. twenty-five primers were designed, and 48 individuals of h. malabaricus from four localities of northwest goiás, in central brazil, were genotyped to characterize the polymorphism at each locus. fourteen primers amplified clearly interpretable products using a single pcr protocol; six loci were polymorphic, but with a low number of alleles per locus (2 or 3). expected heter ...201020690083
negative effects of kudoa islandica n. sp. (myxosporea: kudoidae) on aquaculture and wild fisheries in the early 2000s, experimental rearing of spotted wolffish, anarhichas minor, was started in iceland. health surveillance, carried out at regular intervals during the rearing period, revealed persistent and highly prevalent kudoa infections of fish muscles which caused great financial losses due to post mortem myoliquefaction. in addition, during the traditional process of drying and smoking wild atlantic lumpfish, cyclopterus lumpus, the muscles from some fish almost completely disappear and ...201425161912
differentiation of the xy sex chromosomes in the fish hoplias malabaricus (characiformes, erythrinidae): unusual accumulation of repetitive sequences on the x chromosome.the wolf fish hoplias malabaricus (erythrinidae) presents a high karyotypic diversity, with 7 karyomorphs identified. karyomorph a is characterized by 2n = 42 chromosomes, without morphologically differentiated sex chromosomes. karyomorph b also has 2n = 42 chromosomes for both sexes, but differs by a distinct heteromorphic xx/xy sex chromosome system. the cytogenetic mapping of 5 classes of repetitive dna indicated similarities between both karyomorphs and the probable derivation of the xy chro ...201020502069
mast cells in common wolffish anarhichas lupus l.: ontogeny, distribution and association with lymphatic vessels.the morphology, ontogeny and tissue distribution of mast cells were studied in common wolffish(anarhichas lupus l.) at the larval, juvenile and adult life stages using light and electron-microscopy and immunohistochemistry. fish were sampled at 1 day, 1, 2, 3, 4, 8 and 12 weeks post-hatching in addition to 6 and 9 months and 2 years and older. from 8 weeks post-hatching, mast cells in common wolffish mainly appeared as oval or rounded cells 8-15 mm in diameter with an eccentrically placed, ovoid ...201324436976
atlantic wolf-fish anarhichas lupus population diversity: growth and maturation.biological data from 1125 female atlantic wolf-fish anarhichas lupus were collected during 2002-2006 at their main spawning and fishing grounds in iceland. the results demonstrated substantial annual variation in growth and maturity of female a. lupus. the fast growing females mature earlier than the slow growing ones. in addition, females mature at a larger size and greater age in warmer temperatures than colder ones. there was a strong negative relationship between temperature and growth, whic ...201424410311
purification and partial characterization of vitellogenin from spotted wolffish (anarhichas minor) and development of an enzyme-linked immunosorbent assay for the determination of gender and sexual maturity.vitellogenin (vtg) from spotted wolffish, anarhichas minor, a candidate species for cold-water marine aquaculture, was purified by mgcl₂/edta precipitation followed by a two-step chromatographic procedure. vtg had an apparent molecular mass of 470 kda, as determined by gel filtration, and an amino acid composition similar to those of other teleosts. sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of the purified vtg revealed a major band with a relative molecular weight of 166 ...201423933680
regional variation in fish predation intensity: a historical perspective in the gulf of maine.regional variation in the intensity of fish predation on tethered brittle stars and crabs was measured at 30-33 m depths in the rocky subtidal zone at seven sites representing coastal and offshore regions of the gulf of maine, usa. analysis of covariance comparing the slopes of brittle star survivorship curves followed by multiple comparisons tests revealed five groupings of sites, with significantly greater predation rates in the two offshore than in the three coastal groups. brittle stars teth ...199228313516
the anatomy of the oesophagus, stomach and intestine in common wolffish (anarhichas lupus l.): a basis for diagnostic work and research.the alimentary canal of laboratory-reared common wolffish (anarhichas lupus l.) was studied using light and electron microscopy. in the oesophagus, a simple columnar microvillous epithelium with transport characteristics was observed in addition to the main striated squamous epithelium. an osmoregulatory function is proposed for the simple columnar epithelium, which was supported by wide, thin-walled vessels. in the stomach, a separate type of neck cells was observed leading into the acinar gast ...200011126578
antifreeze protein does not confer cold tolerance to transgenic drosophila antifreeze proteins (afps) have been reported by some researchers to prolong the viability of tissues, organs, and embryos under hypothermic conditions, while others have observed no such effect or even afp-mediated cryotoxicity. we examined the influence of type iii afp from atlantic wolffish on cold tolerance in a whole animal model system, transgenic drosophila. the activity of the afp, transgenically expressed under the transcriptional control of the female-specific yp1 and yp2 promoter ...19958556858
isolation and characterization of tropomyosin from fish muscle.a comprehensive survey of tropomyosin from various fish myotomal muscles is reported. the fish tropomyosins were blocked at the n-terminus and, as expected, were found to be of similar amino acid composition, alpha-helical content (> 90% at 10 degrees c) and molecular weight to other vertebrate striated muscle forms. the tropomyosins of salmonids and herring muscle were noticeably heterogeneous when assessed by 2d-page. the distribution of isoforms was tissue-specific: slow muscle contained alph ...19948205394
putting temperature and oxygen thresholds of marine animals in context of environmental change: a regional perspective for the scotian shelf and gulf of st. lawrence.we conducted a literature review of reported temperature, salinity, ph, depth and oxygen preferences and thresholds of important marine species found in the gulf of st. lawrence and scotian shelf region. we classified 54 identified fishes and macroinvertebrates as important either because they support a commercial fishery, have threatened or at risk status, or meet one of the following criteria: bycatch, baitfish, invasive, vagrant, important for ecosystem energy transfer, or predators or prey o ...201627997536
genetic differentiation among distinct karyomorphs of the wolf fish hoplias malabaricus species complex (characiformes, erythrinidae) and report of unusual hybridization with natural this study, genetic differentiation between karyomorphs a (2n = 42) and d (2n = 39/40) of the wolf fish hoplias malabaricus, which is comprised of several cryptic species that present a wide variety of diploid chromosome numbers and sex chromosome systems, resulting in the identification of seven distinct karyomorphs (a-g), was investigated using a combination of molecular and cytogenetic tools. deep sequence divergences for both karyomorphs were observed and indicate a long period of reprodu ...201425263542
osteodentine and vascular osteodentine of anarhichas lupus (l.).tem, sem and x-ray diffraction analysis demonstrate the heterogeneity of the dentinal tissue on anarhichas lupus, a vascular osteodentine. the disordered aspect of collagen fibres, incompletely mineralized (the periodical striation being still visible), explains the scattered distribution of crystallites since they are responsible for their arrangement. the low degree of mineralization revealed by the visible collagen striation is confirmed by x-ray diffraction analysis (the crystallinity of vas ...1978630586
morphological aspects of clinostomidae metacercariae (trematoda: digenea) in hoplerytrinus unitaeniatus and hoplias malabaricus (pisces: erythrinidae) of the neotropical region, brazil.species of fish of marajó island, state of pará, brazil, were examined to identify the trematodes parasitizing 102 hoplerytrinus unitaeniatus (gold wolf fish) and 104 hoplias malabaricus (thraira). metacercariae of two species of trematodes, 170 specimens of clinostomatopsis sorbens and 10 ithyoclinostomum dimorphum were found and identified. the parasitary indices of c. sorbens from h. unitaeniatus and h. malabaricus, were 43.14% and 30.77% for prevalence, 2.52 and 1.84 for mean intensity, 1.09 ...201424838543
Displaying items 1 - 37 of 37