variation in the development of two isolates of cryptocaryon irritans.two isolates of cryptocaryon irritans obtained from acanthopagrus australis from moreton bay (isolate c1) and gymnocranius audleyi from heron island (isolate c2) were passaged on lates calcarifer and macquaria novemaculeata at 20 and 25 c under identical laboratory conditions. there were significant differences between isolates in the diameter of trophonts and tomonts, the incubation period of tomonts, and the length of theronts. trophonts of c1 were significantly larger on l. calcarifer than on ...19968636840
antigenic relationship of the picorna-like virus of larval barramundi, lates calcarifer bloch to the nodavirus of larval striped jack, pseudocaranx dentex (bloch & schneider). 19947726766
ontogeny of immunoreactive steroidogenic proteins (adrenodoxin and cytochrome p-450(21)) in the interrenals of the teleost lates calcarifer.antisera against bovine adrenodoxin and cytochrome p-450(21) (steroid 21-hydroxylase) cross-reacted with the interrenal cells of the adult asian seabass (lates calcarifer); the cells were arranged as cords, two cells thick, in the headkidney. during larval development, cells immunoreactive for adrenodoxin were first observed 1 day posthatching (dph); immunoreactivity for cytochrome p-450(21) was first detected at 1.5 dph. initially, the interrenal cells occurred as a mass in each headkidney, whi ...19968860319
characterization of an insulin-like growth factor (igf) receptor and the insulin-like effects of igf-1 in the bony fish, lates calcarifer.the present work is part of a broad phylogenetic study of the insulin superfamily of peptides in lower vertebrates. in the bony fish barramundi (lates calcarifer), the presence of igf receptors were investigated in the liver by means of competitive binding studies. the results suggested the presence of a type 1-like but no type 2-like igf receptor. we also demonstrated insulin-like effects of intraperitoneally injected recombinant human (rh)-igf-1 in barramundi with rh-igf-1 and rh-insulin showi ...19979163581
characterization of the seabass pancreatic alpha-amylase gene and promoter.seabass (lates calcarifer) pancreatic alpha-amylase gene was cloned and characterized. the alpha-amylase cdna has 1620 bp and the deduced polypeptide has 522 amino acids. southern blot indicated that there are two gene copies in the seabass genome. sequence analysis showed that except for the loss of an intron in seabass, the coding region and the exon/intron boundaries are highly homologous to those of mammalian amylases. however, the promoter regions are distinctively divergent. to investigate ...200415094338
hormonal influence on amylase gene expression during seabass (lates calcarifer) larval development.alpha-amylase gene expression was detected in newly hatched seabass (lates calcarifer) larvae and peaked at around first feeding. this suggests a greater importance of carbohydrates during early larval development than might be expected for carnivorous fishes. in vivo cortisol and triiodothyronine (t(3)) treatment of seabass larvae upregulated alpha-amylase gene expression. the identification of a functional glucocorticoid-response element (gre) on the amylase gene promoter indicates that cortis ...200415242747
first published record of the pathogenic monogenean parasite neobenedenia melleni (capsalidae) from australia.the monogenean neobenedenia melleni (maccallum, 1927) yamaguti 1963 is a well-known and virulent pathogen in culture conditions recorded from the skin of many teleost fish species worldwide. until now, n. melleni has not been reported from wild or cultured fish in australian waters. this study documents a recent outbreak of n. melleni that occurred on lates calcarifer (barramundi) cultivated in sea cages in hinchinbrook channel between hinchinbrook island and mainland queensland, australia, whic ...200111592706
comparison of recombinant barramundi and human insulin-like growth factor (igf)-i in juvenile barramundi (lates calcarifer): in vivo metabolic effects, association with circulating igf-binding proteins, and tissue localisation.the in vivo actions of human and fish insulin-like growth factor (igf)-i have been compared to extend the understanding of the metabolism of igfs in fish and to identify potential differences in their actions. the effects of acute administration of these proteins on the incorporation of glucose into muscle glycogen and leucine into liver protein in juvenile barramundi were investigated. in these in vivo metabolic assays, both baramundi igf-i (bigf-i) and human igf-i (higf-i) increase the incorpo ...200010764550
the influence of fish cage aquaculture on pelagic carbon flow and water chemistry in tidally dominated mangrove estuaries of peninsular malaysia.the impact of floating net cages culturing the seabass, lates calcarifer, on planktonic processes and water chemistry in two heavily used mangrove estuaries in malaysia was examined. concentrations of dissolved inorganic and particulate nutrients were usually greater in cage vs. adjacent (approximately 100 m) non-cage waters, although most variability in water-column chemistry related to water depth and tides. there were few consistent differences in plankton abundance, production or respiration ...200312517423
immune responses of barramundi, lates calcarifer (bloch), after administration of an experimental vibrio harveyi bacterin by intraperitoneal injection, anal intubation and immersion.barramundi, lates calcarifer (bloch), were immunized with an experimental vibrio harveyi bacterin via intraperitoneal injection, immersion and anal intubation. both specific and non-specific immune parameters were measured to compare responses to bacterin after delivery by various methods. elevated antibody activities in sera were found in all treatment groups with barramundi injected intraperitoneally displaying significantly higher antibody activity than the other groups. in addition, there wa ...200415509257
beneficial effects of fish liver preparations of sea bass (lates calcarifer) versus gemfibrozil in high fat diet-induced lipid-intolerant rats.abnormal lipid metabolism is a main cause of dyslipidemia, which is a major risk factor for coronary heart disease and obesity and is even linked to diabetic-dyslipidemic complications. fifteen days of high-fat feeding in charles foster rats resulted in a significant increase in baseline serum lipid levels accompanied by pronounced dyslipidemia. treatment with fish liver preparations (flps) from sea bass and the standard drug gemfibrozil produced a lowering of serum lipids and glucose levels, al ...200312935323
localization of insulin-like growth factor-i immunoreactivity in larval and juvenile barramundi (lates calcarifer).antisera to mammalian igfs cross-react with several fish species, suggesting a long phylogenetic history of igf-like peptides and their functional importance to all vertebrates. in this study, the tissue distribution of igf-i-immunoreactivity (igf-i-ir) was studied at various larval and juvenile stages of the life cycle of barramundi (lates calcarifer). it was shown that the distribution of igf-i-ir in this species was tissue-specific and age-dependent. in newly hatched larvae, presumptive muscu ...19958775055
bacterial vaccines for fish--an update of the current situation worldwide.during the last few years, the use of vaccines for disease prevention in aquaculture has expanded both with regard to the number of fish species and number of microbial diseases. according to the responses to a questionnaire received from 41 countries, vaccination is used in the commercial aquaculture of species like atlantic salmon (salmo salar), rainbow trout (oncorhynchus mykiss), sea bass (dicentrarchus labrax), sea bream (sparus aurata), barramundi (lates calcarifer), tilapia (tilapia spp), ...200515962470
nodavirus infection causes mortalities in hatchery produced larvae of lates calcarifer: first report from india.larvae (15 to 21 d post hatch, dph) of the asian sea bass lates calcarifer (bloch) suffered heavy mortalities (60 to 90%) during the hatchery-rearing phase. darkened and moribund larvae showed no evidence of bacterial or parasitic infections. tissue sections of brain and spinal cord showed clear necrotic vacuolation. electron microscopy revealed membrane-bound viral particles in the cytoplasm of the nerve cells. the viral particles measured 28 to 30 nm in diameter. primer sets, designed for the ...200515819426
response and function of cutaneous mucosal and serum antibodies in barramundi (lates calcarifer) acclimated in seawater and freshwater.mucosal and serum antibody responses were studied in sibling barramundi (lates calcarifer) acclimated in either seawater or freshwater following vaccination by intraperitoneal injection or direct immersion in an inactivated streptococcus iniae vaccine. as expected, route of vaccination had a marked effect on immune response, with direct immersion resulting in low serum antibody levels against s. iniae by elisa detected 21 days post vaccination at 26 degrees c, whilst a significant response was d ...200616337812
skeletal myopathy in juvenile barramundi, lates calcarifer (bloch), cultured in potassium-deficient saline groundwater.saline groundwater is being pumped from a number of locations in rural western australia to prevent secondary salinity impacting farmland, rural infrastructure and areas with high conservation value. aquaculture may offset the costs of groundwater pumping, and the suitability of groundwater for finfish aquaculture is being assessed through bioassays. there are marked spatial variations in the ionic composition of saline ground water in western australia and this paper describes two bioassays inv ...200415357711
description of pseudorhabdosynochus seabassi sp. n. (monogenea: diplectanidae) from lates calcarifer and revision of the phylogenetic position of diplectanum grouperi (monogenea: diplectanidae) based on rdna sequence data.pseudorhabdosynochus seabassi sp. n. (monogenea: diplectanidae) from the gill filaments of lates calcarifer bloch, a marine teleost fish held in floating sea cages in guangdong province, china, is described based on morphological observations and molecular data. the shapes of the male copulatory organs (mco) of pseudorhabdosynochus spp. were the focus of this study. the typical proximal part of the mco in most species of pseudorhabdosynochus is reniform, heavily sclerotized, and divided into fou ...200516270804
a systemic iridoviral disease in mullet, mugil cephalus l., and tiger grouper, epinephelus fuscoguttatus forsskal: a first report and study.a systemic iridoviral disease associated with high mortality was initially recognized in cultured mullet, mugil cephalus l., and tiger grouper, epinephelus fuscoguttatus forsskal, by histopathology and transmission electron microscopy. polymerase chain reaction was performed on tissues and viral isolates, using four published primer sets developed for the red sea bream iridovirus (rsiv). an indirect fluorescent antibody test was also performed on virus-infected atcc gruntfin (gf) and seabass, la ...200415575877
a novel 'skinny pot-belly' disease in asian seabass fry, lates calcarifer (bloch). 200415575881
lernanthropid copepods (siphonostomatoida) parasitic on fishes of the gulf of thailand.five species of copepods (siphonostomatoida: lernanthropidae) parasitic on fishes of the gulf of thailand are reported. they are: lernanthropus corniger yamaguti, 1954 from megalaspis cordyla (linnaeus); l. latis yamaguti, 1954 from lates calcarifer (bloch); l. nemipteri jayasree & pillai, 1976 from scolopsis lineata quoy et gaimard; l. sillaginis pillai, 1963 from priacanthus tayenus richardson; and norion tayenus n. sp. from p. tayenus. except for l. sillaginis, the remaining four species are ...200415084830
the assessment of vitellogenin as a biomarker of exposure to estrogenic compounds in two australian perciformes.vitellogenin (vtg) is a yolk protein precursor that has been identified as a sensitive biomarker for exposure to estrogenic compounds. we evaluated specific monoclonal and polyclonal antibodies for reactivity with plasma vtg from two australian perciformes, the tropical barramundi (lates calcarifer) and the temperate black bream (acanthopagrus butcheri). blood plasma from 17beta-estradiol exposed (e2) male barramundi (20 mg kg(-1)) and male black bream (2.5-5.0 mg kg(-1)) were sent to biosense l ...200818377978
establishment of embryonic cell line from sea bass (lates calcarifer) for virus isolation.a continuous cell line was established from blastula stage embryos of sea bass (lates calcarifer). the sea bass embryonic cells were maintained in leibovitz's l-15 supplemented with 15% fetal bovine serum. the embryonic cell line was sub-cultured more than 70 passages over a period of 1.5 years and is designated as sahul indian sea bass embryonic (sise) cell line. the cells were able to grow at temperatures between 25 and 32 degrees c with an optimum temperature of 28 degrees c. the growth rate ...200616919787
protective efficiency of dna vaccination in asian seabass (lates calcarifer) against vibrio anguillarum.vibriosis is one of the most prevalent fish diseases caused by bacteria belonging to the genus vibrio. vibriosis caused by vibrio anguillarum produces a 38-kda major outer membrane porin protein (omp) for biofilm formation and bile resistant activity. the gene encoding the porin was used to construct dna vaccine. the protective efficiency of such vaccine against v. anguillarum causing acute vibrio haemorrhagic septicaemia was evaluated in asian seabass (lates calcarifer bloch), a common species ...200717337208
laticola dae n. sp. (monogenea: diplectanidae) from epinephelus maculatus (perciformes: serranidae) off new caledonia.laticola dae n. sp. is described from specimens collected from the gill-filaments of the highfin grouper epinephelus maculatus, a coral reef fish caught off nouméa, new caledonia, south pacific. the species is characterised by a spoon-shaped sclerotised male copulatory organ, with four thin walls and 73-[formula: see text]m in outer length, and a sclerotised vagina in form of a disc, 16-[formula: see text]m in diameter, with a smaller hemisphere on one side. laticola yang et al., 2006 was descri ...200616786282
anti-oxidant status in embryonic, post-hatch and larval stages of asian seabass (lates calcarifer).the concentrations of anti-oxidant enzymes such as superoxide dismutase (sod), catalase (cat) and selenium-dependent glutathione peroxidase (segpx), and low molecular weight free-radical scavengers such as reduced glutathione (gsh) and ascorbic acid (vitamin c) were evaluated during the period from gastrulation (gs) to 25 days post-hatch (dph) in the larvae of asian seabass, lates calcarifer. oxidative damage due to lipid peroxidation (lpo) was also assessed, by evaluation of the formation of ma ...200818649032
pcr amplification and sequence analysis of the major capsid protein gene of megalocytiviruses isolated in taiwan.viruses belonging to the genus megalocytivirus in the family iridoviridae are one of the major agents causing mass mortalities in marine and freshwater fish in asian countries. outbreaks of iridovirus disease have been reported among various fish species in taiwan. however, the genotypes of these iridoviruses have not yet been determined. in this study, seven megalocytivirus isolates from four fish species: king grouper, epinephelus lanceolatus (bloch), barramundi perch, lates calcarifer (bloch) ...200919460084
trace metal dynamics in fishes from the southwest coast of india.the concentration of fe, co, ni, cu, zn, cd, and pb in the muscle tissue of marine fishes like lates calcarifer, nemipterus japonicus, caranx melampygus, rastrelliger kanagurta, and cyanoglossus macrostomus was estimated from samples collected in the continental shelf waters off kochi and mangalore on southwest coast of india. species-specific and spatially heterogeneous patterns of tissue metals loads were apparent within the pelagic and demersal fish species for the two regions. the concentrat ...201019543989
cloning and expression analysis of cathepsin d in the olive flounder paralichthys olivaceus.we isolated a homolog of cathepsin d from the cdna library of the olive flounder kidney. the olive flounder cathepsin d transcript consisted of 1,733 bp that encoded a polypeptide of 396 amino acids. the overall similarity between olive flounder cathepsin d and other cathepsin ds was very high, with the highest amino acid sequence identity to barramundi perch (89%). rt-pcr revealed that cathepsin d was expressed in almost all tissues, with high expression in the liver, intestine, kidney, skin, a ...200919661707
molecular cloning, characterization and expression analysis of cathepsin d gene from turbot scophthalmus maximus.cathepsin d is a lysosomal endoproteolytic aspartic proteinase which also has been found in endosomes of macrophage. it is thought to play key roles in the developmental and physiological process of animals. the est sequence of turbot (scophthalmus maximus l.) cathepsin d was obtained from a subtractive cdna library. in the present study, 5'-race and 3'-race were carried out to obtain the complete cdna sequence of turbot cathepsin d, which contained a 91 bp 5'-utr, a 1191 bp open reading frame e ...200918948209
influence of physico-chemical properties on the abundance of a few economically important juvenile fin-fishes of vellar estuary.studies on the economically important juvenile fin-fishes such as elops machnata, chanos chanos, lates calcarifer, epinephelus sp., sillago sihama, etroplus suratensis, mugil cephalus, liza parsia and liza tade with relation to the hydrographical parameters as rainfall, temperature, salinity, dissolved oxygen and ph of vellar estuary during september 2001 to august 2002. the simple correlation co-efficient showed positive significance against juvenile density with water temperature and dissolved ...200516114469
phylogenetic analysis of betanodavirus isolates from australian australia, disease caused by betanodavirus has been reported in an increasing number of cultured finfish since the first report of mortalities in 1990. partial coat protein gene sequences from the t2 or t4 regions of 8 betanodaviruses from barramundi lates calcarifer, sleepy cod oxyeleotris lineolata, striped trumpeter latris lineata, barramundi cod cromileptes altivelis, australian bass macquaria novemaculata and gold-spotted rockcod epinephelus coioides from several australian states were d ...200920099409
identification and verification of qtl associated with growth traits in two genetic backgrounds of barramundi (lates calcarifer).quantitative trait loci (qtl) affecting growth traits have previously been mapped in linkage groups (lg) 2, 3 and 23 of barramundi (lates carcalifer), but these qtl have not been verified in different genetic backgrounds and environments. here, we report the identification and verification of qtl for growth traits on lg2, 3, 10 and 23 in f(1) families constructed using brooders from the singapore marine aquaculture center (mac) and from wild stocks collected in thailand (thai). the previously de ...200818076743
a standard panel of microsatellites for asian seabass (lates calcarifer).microsatellites are the most popular markers for parentage assignment and population genetic studies. to meet the demand for international comparability for genetic studies of asian seabass, a standard panel of 28 microsatellites has been selected and characterized using the dna of 24 individuals from thailand, malaysia, indonesia and australia. the average allele number of these markers was 10.82 +/- 0.71 (range: 6-19), and the expected heterozygosity averaged 0.76 +/- 0.02 (range: 0.63-1.00). ...201019793264
identification of vibrio harveyi isolated from diseased asian seabass lates calcarifer by use of 16s ribosomal dna sequencing.the grow out of asian seabass lates calcarifer in marine net-cages is a popular aquaculture activity in malaysia. production of this species is greatly affected by the occurrence of vibriosis, which causes heavy mortality. generally, young fish are more susceptible; they exhibit anorexia and skin darkening, followed by heavy mortality. the acutely affected older fish may also exhibit bloody lesions around the anus and the base of the fins. twenty-one bacterial isolates obtained from internal org ...200920043399
molecular characterization and expression analysis of cathepsin b and l cysteine proteases from rock bream (oplegnathus fasciatus).cathepsins are lysosomal cysteine proteases of the papain family that play an important role in intracellular protein degradation and turn over within the lysosomal system. in the present study, full-length sequences of cathepsin b (rbcathepsin b) and l (rbcathepsin l) were identified after transcriptome sequencing of rock bream oplegnathus fasciatus mixed tissue cdna. cathepsin b was composed of 330 amino acid residues with 36 kda predicted molecular mass. rbcathepsin l contained 336 amino acid ...201021195770
potential use of chitosan nanoparticles for oral delivery of dna vaccine in asian sea bass (lates calcarifer) to protect from vibrio (listonella) recent years, attention has been focused on the possibility of utilizing dna vaccines in fish aquaculture. a successful regime for intramuscular injection of naked dna into fish has been developed and novel methods to deliver this dna to fish are under investigation. the potential of chitosan as a polycationic gene carrier for oral administration has been explored since 1990s. the present study examines the potential efficacy of dna vaccine against vibrio anguillarum through oral route using ...200818479939
characterization of apoptosis induced by grouper iridovirus in two newly established cell lines from barramundi, lates calcarifer (bloch).two new cell lines have been established from the muscle and swim bladder tissues of barramundi, lates calcarifer, and designated as bm (barramundi muscle) and bsb (barramundi swimbladder), respectively. the cells multiplied well at 28 degrees c in leibovitz's l-15 medium supplemented with 10% foetal bovine serum, and have been continuously subcultured more than 100 times to date. morphologically, bm cells were mostly fibroblastic, whereas bsb were mostly epithelial. both cell lines were suscept ...200819238757
a high-resolution linkage map for comparative genome analysis and qtl fine mapping in asian seabass, lates calcarifer.high density linkage maps are essential for comparative analysis of synteny, fine mapping of quantitative trait loci (qtl), searching for candidate genes and facilitating genome sequence assembly. however, in most foodfish species, marker density is still low. we previously reported a first generation linkage map with 240 dna markers and its application to preliminarily map qtl for growth traits in asian seabass (lates calcarifer). here, we report a high-resolution linkage map with 790 microsate ...201121457569
investigation of highly unsaturated fatty acid metabolism in the asian sea bass, lates calcarifer.lates calcarifer, commonly known as the asian sea bass or barramundi, is an interesting species that has great aquaculture potential in asia including malaysia and also australia. we have investigated essential fatty acid metabolism in this species, focusing on the endogenous highly unsaturated fatty acid (hufa) synthesis pathway using both biochemical and molecular biological approaches. fatty acyl desaturase (fad) and elongase (elovl) cdnas were cloned and functional characterization identifie ...201020532815
identification and analysis of a prepro-chicken gonadotropin releasing hormone ii (preprocgnrh-ii) precursor in the asian seabass, lates calcarifer, based on an est-based assessment of its brain transcriptome.using a novel library of 5637 expressed sequence tags (ests) from the brain tissue of the asian seabass (lates calcarifer), we first characterized the brain transcriptome for this economically important species. the ests generated from the brain of l. calcarifer yielded 2410 unique transcripts (uts) which comprise of 982 consensi and 1428 singletons. based on database similarity, 1005 uts (41.7%) can be assigned putative functions and were grouped into 12 functional categories related to the bra ...200818280674
normalizing rt-qpcr data: are we getting the right answers? an appraisal of normalization approaches and internal reference genes from a case study in the finfish lates calcarifer.commonly used normalization approaches for quantitative reverse transcription polymerase chain reaction analyses include (a) input nucleic acids standardization (δc (q) method), (b) normalizing target gene transcript abundance against a single internal reference gene (δδc (q) method), and (c) geometric averaging of multiple reference gene abundance using the genorm software. we compared these three approaches to examine expression of a negative muscle growth regulator gene, myostatin-i (mstn-i), ...201120309600
two hepcidin-like antimicrobial peptides in barramundi lates calcarifer exhibit differing tissue tropism and are induced in response to lipopolysaccharide.genes encoding two hepcidin-like antimicrobial peptides were discovered in barramundi, lates calcarifer (barramundi, giant sea perch). analysis of the coding regions indicated that genes for each hepcidin comprised 3 exons and 2 introns. the deduced amino acid sequences for each molecule resulted in a protein comprising a signal sequence of 24 aa in each case, coupled to a prepropeptide of 75 aa for hepcidin 1 and 78 aa for hepcidin 2. a cleavage site was identified in each prepropetide at amino ...201121664976
intracellular copper zinc superoxide dismutase (iccuznsod) from asian seabass (lates calcarifer): molecular cloning, characterization and gene expression with reference to vibrio anguillarum infection.copper zinc superoxide dismutase (cuznsod) is the family of most important antioxidant metalloenzymes that protects tissues from damage by reactive oxygen species (ros). in the present study, the intracellular copper zinc sod from the asian seabass lates calcarifer (lc-iccuznsod) was identified by rna ligase mediated rapid amplification of cdna ends (rlm-race) technique. the full-length cdna of lc-iccuznsod consisted of 809 nucleotides with an open-reading frame of 465bp encoding 154 amino acids ...201122101033
infection and immunization trials of asian seabass (lates calcarifer) against fish pathogen vibrio anguillarum.vibriosis is one of the most prevalent fish diseases caused by bacteria belonging to the genus vibrio sp. fish disease will be controlled by proper vaccination trials and maintenance of fish form. pathogenicity for asian seabass (lates calcarifer) against v anguillarum results in necrosis and haemorrhagic areas near the base of fins, exopthalmia and ulcers on the skin surface. around 50, 100, 200 microl of formalin killed bacterial cells were injected (intraperitoneal) to three different size (5 ...201021186732
permanent genetic resources added to molecular ecology resources database 1 october 2010-30 november 2010.this article documents the addition of 277 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: ascochyta rabiei, cambarellus chapalanus, chionodraco hamatus, coptis omeiensis, cynoscion nebulosus, daphnia magna, gerbillus nigeriae, isurus oxyrinchus, lates calcarifer, metacarcinus magister, oplegnathus fasciatus, pachycondyla verenae, phaethon lepturus, pimelodus grosskopfii, rotylenchulus reniformis, scomberomorus niphonius, sep ...201121429157
analysis of two lysozyme genes and antimicrobial functions of their recombinant proteins in asian seabass.lysozymes are important proteins of the innate immune system for the defense against bacterial infection. we cloned and analyzed chicken-type (c-type) and goose-type (g-type) lysozymes from asian seabass (lates calcarifer). the deduced amino acid sequence of the c-type lysozyme contained 144 residues and possessed typical structure residues, conserved catalytic residues (glu(50) and asp(67)) and a "gstdygifqins" motif. the deduced g-type lysozyme contained 187 residues and possessed a goose egg ...201324244553
identification and characterization of 63 micrornas in the asian seabass lates calcarifer.micrornas (mirnas) play an important role in the regulation of many fundamental biological processes. so far mirnas have been only identified in a few fish species, although there are over 30,000 fish species living under different environmental conditions on the earth. here, we described an approach to identify conserved mirnas and characterized their expression patterns in different tissues for the first time in a food fish species asian seabass (lates calcarifer).201121412421
transcriptome analysis of the cryptocaryon irritans tomont stage identifies potential genes for the detection and control of cryptocaryonosis.cryptocaryon irritans is a parasitic ciliate that causes cryptocaryonosis (white spot disease) in marine fish. diagnosis of cryptocaryonosis often depends on the appearance of white spots on the surface of the fish, which are usually visible only during later stages of the disease. identifying suitable biomarkers of this parasite would aid the development of diagnostic tools and control strategies for c. irritans. the c. irritans genome is virtually unexplored; therefore, we generated and analyz ...201020113487
development and partial characterization of new marine cell line from brain of asian sea bass lates calcarifer for virus isolation.a new cell line, asian sea bass brain (asbb), was derived from the brain tissue of asian sea bass lates calcarifer. this cell line was maintained in leibovitz l-15 media supplemented with 10% fetal bovine serum (fbs). the asbb cell line was subcultured more than 60 times over a period of 15 mo. the asbb cell line consists predominantly of fibroblastic-like cells and was able to grow at temperatures between 20°c and 30°c with an optimum temperature of 25°c. the growth rate of these cells increase ...201021082288
methyl mercury in fish-a case study on various samples collected from ganges river at west bengal.this study investigated the presence of total mercury (hg) and organic mercury levels in the muscle of 19 common fresh water fish species captured from river ganges, west bengal, india. the total mercury level found in our study may not cause any toxic effect, but the methyl mercury (mehg) level in some freshwater fish species was surprisingly very high and toxically unacceptable. the results of mercury analysis in various specimens indicated that some fish muscles tended to accumulate high leve ...201121713467
comparative study on molecular characteristics of acid soluble collagens from skin and swim bladder of seabass (lates calcarifer).acid soluble collagens (ascs) from skin and swim bladder of seabass (lates calcarifer) were isolated and comparatively characterised. higher yield (28.5%) was obtained for asc from swim bladder, compared with that from skin (15.8%). ascs from both skin and swim bladder had the similar protein patterns and were identified to be type i. both α- and β-chains constituted as major components. fourier transform infrared (ftir) spectra revealed that both ascs were triple helix in structure. asc from bo ...201223497906
optimisation and validation of a real-time reverse transcriptase-polymerase chain reaction assay for detection of betanodavirus.a rt-qpcr assay that was developed and optimised for detection of betanodaviruses was validated for use as a diagnostic test for viral nervous necrosis disease of fish. four betanodavirus genotypes were detected but the sensitivity was greatest for redspotted grouper nervous necrosis virus (rgnnv). the analytical sensitivity was 10-1000-fold greater than that of a nested rt-pcr assay and the limit of detection was <0.4 tcid(50) units per reaction. the assay was highly repeatable (standard deviat ...201019891987
molecular characterization, tissue expression and sequence variability of the barramundi (lates calcarifer) myostatin gene.myostatin (mstn) is a member of the transforming growth factor-beta superfamily that negatively regulates growth of skeletal muscle tissue. the gene encoding for the mstn peptide is a consolidate candidate for the enhancement of productivity in terrestrial livestock. this gene potentially represents an important target for growth improvement of cultured finfish.200818282302
immune responses of asian sea bass, lates calcarifer bloch, against an inactivated betanodavirus vaccine.asian sea bass, lates calcarifer (bloch), exhibited strong immune responses against a single injection of the formalin-inactivated red-spotted grouper nervous necrosis virus (rgnnv), a betanodavirus originally isolated in japan. fish produced neutralizing antibodies at high titre levels from days 10 (mean titre 1:480) to 116 (1:1280), with the highest titre at day 60 post-vaccination (1:4480). when fish were challenged with the homologous rgnnv at day 54 post-vaccination, there were no mortaliti ...200919364387
host-directed evolution of a novel lactate oxidase in streptococcus iniae isolates from barramundi (lates calcarifer).in streptococcus iniae, lactate metabolism is dependent upon two proteins, lactate permease that mediates uptake and lactate oxidase, a flavin mononucleotide-dependent enzyme that catalyzes oxidation of alpha-hydroxyacids. a novel variant of the lactate oxidase gene, lcto, in australian isolates of s. iniae from diseased barramundi was found during a diagnostic screen using lox-1 and lox-2 primers, yielding amplicons of 920 bp instead of the expected 869 bp. sequencing of the novel gene variant ...200919270123
pathogenicity of aeromonas hydrophila isolated from the malaysian sea against coral (turbinaria sp.) and sea bass (lates calcarifer).a study was carried out to determine the pathogenicity (hemolytic activity) on corals (turbinaria sp.) and sea bass (lates calcarifer) of aeromonas hydrophila from water, sediment, and coral. samples were collected from coastal water and coral reef areas. one hundred and sixty-two isolates were successfully isolated. out of 162, 95 were from seawater, 49 from sediment, and 18 from coral. sixteen isolates were picked and identified. isolates were identified using a conventional biochemical test, ...201627221587
isolation and partial characterization of asian sea bass (lates calcarifer) vitellogenin.a study was conducted to isolate, partial characterize asian sea bass (lates calcarifer) vitellogenin (vtg). two-year-old juvenile l. calcarifer (n = 10) were given three intraperitoneal injections of 17-β estradiol (e2) at a dose of 2 mg/kg body weight to induce vitellogenesis. blood was collected 3 days after the last injection, and plasma was purified through gel filtration chromatography. a broad single symmetrical peak consisting of vtg molecule was produced. protein concentration was 0.059 ...201222878544
comparative characterization of a temperature responsive gene (lactate dehydrogenase-b, ldh-b) in two congeneric tropical fish, lates calcarifer and lates niloticus.the characterization of candidate loci is a critical step in obtaining insight into adaptation and acclimation of organisms. in this study of two non-model tropical (to sub-tropical) congeneric perciformes (lates calcarifer and lates niloticus) we characterized both coding and non-coding regions of lactate dehydrogenase-b (ldh-b), a locus which exhibits temperature-adaptive differences among temperate and sub-tropical populations of the north american killifish fundulus heteroclitus. ldh-b was 5 ...200919787021
isolation and characterization of a nodavirus associated with mass mortality in asian seabass (lates calcarifer) from the west coast of india.a fish nodavirus was detected in the juveniles of asian seabass (lates calcarifer) during a massive outbreak in the seabass cage culture farm located in the south west coast of india. the clinical signs of the disease included anorexia, inflated abdomen, exophthalmia, darkening of the whole body, erratic swimming and cork-screw type movement followed by death. the dead and the moribund fish were analyzed for nodavirus by reverse transcriptase-polymerase chain reaction (rt-pcr) using specific pri ...201425674617
development of a pluripotent es-like cell line from asian sea bass (lates calcarifer)--an oviparous stem cell line mimicking viviparous es cells.we report a pluripotent embryonic stem cell-like cell line designated as sbes from blastula stage embryos of asian sea bass (lates calcarifer), which is an economically important cultivable and edible marine fish species in india. the sbes cells were cultured at 28 degrees c in leibovitz l-15 medium supplemented with 20% fetal bovine serum without a feeder layer. the es-like cells were round or polygonal and grew exponentially in culture. the sbes cells exhibited an intense alkaline phosphatase ...200717704967
screening probiotic candidates for a mixture of probiotics to enhance the growth performance, immunity, and disease resistance of asian seabass, lates calcarifer (bloch), against aeromonas hydrophila.six bacteria, including, lactobacillus casei m15, lac. plantarum d8, lac. pentosus bd6, lac. fermentum lw2, enterococcus faecium 10-10, and bacillus subtilis e20, and one yeast, saccharomyces cerevisiae p13 were selected as probiotics for asian seabass, lates calcarifer, by tracking the growth performance and disease resistance of fish against aeromonas hydrophila in the first trial. the probiotic efficiency screening results showed that b. subtilis e20 and lac. pentosus bd6, and s. cerevisiae p ...201727838565
detection of betanodavirus in juvenile barramundi, lates calcarifer (bloch), by antigen capture elisa.betanodavirus infection of fish has been responsible for mass mortalities in aquaculture hatcheries worldwide. betanodaviruses possess a bipartite single-stranded rna genome consisting of the 3.1 kb rna1 encoding an rna-dependent rna polymerase and the b2 protein, while the 1.4 kb rna2 encodes the viral nucleocapsid protein, alpha. a panel of six monoclonal antibodies against the alpha protein of greasy grouper nervous necrosis virus (ggnnv) was developed for use in diagnostics. all antibodies r ...200616866926
improvement in non-specific immunity and disease resistance of barramundi, lates calcarifer (bloch), by diets containing daphnia similis meal.a 42-day study was conducted with barramundi, lates calcarifer, to evaluate the effects of daphnia meal derived from daphnia similis on fish growth, immune response, and disease resistance to aeromonas hydrophila. three isonitrogenous (45%) and isolipid (10%) experimental diets were formulated to contain 0% (control), 5% (d5), and 10% (d10) daphnia meal. growth was depressed when fish were fed with the d10 diet for 42 days compared to control. however, the growth in fish fed with control and d5 ...201525681749
persistently betanodavirus-infected barramundi (lates calcarifer) exhibit resistances to red sea bream iridovirus infection.nervous necrosis virus (nnv) and red sea bream iridovirus (rsiv) are two important pathogens that have caused acute, highly contagious, and widespread diseases among wild and cultured fish, especially at larval and juvenile stages. we discovered that the pathogenicity of nnv to the 80 days post-hatch (dph) barramundi is lower than that to the 14 dph barramundi. following nnv challenge, no mortality occurred in the 80 dph barramundi, but nnv rna2 and barramundi mx (bmx) gene expression was detect ...201323831937
age dependency of nervous necrosis virus infection in barramundi lates calcarifer (bloch).age-dependent susceptibility to nervous necrosis virus (nnv) was demonstrated for barramundi (lates calcarifer). the experiment used juvenile barramundi produced from a single spawning that were challenged consecutively by immersion with a redspotted grouper nervous necrosis virus (rgnnv) isolate. the dose and environmental conditions (35 ppt salinity and 30 °c) were constant. fish and water were sampled longitudinally for histopathology and rt-qpcr analysis to examine the evolution of the disea ...201728117491
persistence of betanodavirus in barramundi brain (bb) cell line involves the induction of interferon response.the bb cell line derived from the brain tissue of a barramundi (lates calcarifer) that survived nervous necrosis virus (nnv) infection is persistently infected with nnv. to elucidate whether interferon (ifn) plays a role in the mechanism of nnv-persistent infection in bb cell line, a virus-negative control cell line was obtained by treating bb cells with nnv-specific rabbit antiserum for 5 subcultures. after the treatment, nnv titer or rna or capsid protein was no longer detected in the cured bb ...200616698284
sequence analysis of coat protein gene of wuhan nodavirus isolated from insect.wuhan nodavirus (whnv) particles are isometric, non-enveloped, and about 29 nm in diameter. in the previous study, we determine its physiochemical characterization and the nucleotide sequence of the larger genomic segment, rna1 and identify it a nodavirus. whnv rna1 is 3,149 nt in length, encoding protein a, catalytic subunit of rna-dependent rna polymerase (rdrp). in this report, we complete the sequence determination of the smaller genomic segment, rna2 of whnv. whnv rna2 is determined to be 1 ...200616644053
molecular evidence for association of chlamydiales bacteria with epitheliocystis in leafy seadragon (phycodurus eques), silver perch (bidyanus bidyanus), and barramundi (lates calcarifer).epitheliocystis in leafy seadragon (phycodurus eques), silver perch (bidyanus bidyanus), and barramundi (lates calcarifer), previously associated with chlamydial bacterial infection using ultrastructural analysis, was further investigated by using molecular and immunocytochemical methods. morphologically, all three species showed epitheliocystis cysts in the gills, and barramundi also showed lymphocystis cysts in the skin. from gill cysts of all three species and from skin cysts of barramundi 16 ...200616391055
growth, enzymatic glutathione peroxidase activity and biochemical status of juvenile barramundi (lates calcarifer) fed dietary fermented soybean meal and organic selenium.solvent-extracted soybean meal (sbm) was fermented using baker's yeast saccharomyces cerevisae at 30 °c for 5 days. four isonitrogenous and isocaloric diets containing 75% sbm protein, either fermented or non-fermented (sbm and fsbm), and supplemented or not with organic se (os) (sbmos and fsbmos), were fed to triplicate groups of juvenile barramundi (lates calcarifer) (initial weight of 5 g) for 75 days. a fishmeal (fm)-based diet formulated for juvenile barramundi was used as a reference diet. ...201628028742
comparative analysis of cscu/znsod defense role by molecular characterization: gene expression-enzyme activity-protein (copper/zinc superoxide dismutase) primarily scavenges cytosolic reactive oxygen species (ros) by converting ros to hydrogen peroxide, which is then converted to water by the catalytic action of catalase, thus playing a pivotal role in the first line of defense mechanism against oxidative stress. in this study, we have reported a complete molecular characterization of cdna sequence from striped murrel channa striatus (cs). cellular location prediction reveals that cscu/znsod protein is ...201525804520
barramundi (lates calcarifer) desaturase with δ6/δ8 dual activities.barramundi is a commercially farmed fish in australia. to examine the potential for barramundi to metabolise dietary α-linolenic acid (ala, 18:3 n-3), the existence of barramundi desaturase enzymes was examined. a putative fatty acid δ6 desaturase was cloned from barramundi liver and expressed in yeast. functional expression revealed δ6 desaturase activity with both the 18 carbon (c(18)) and c(24) n-3 fatty acids, ala and 24:5 n-3 as well as the c(18) n-6 fatty, linoleic acid (la, 18:2 n-6). met ...201222391738
a divalent dna vaccine based on sia10 and ompu induces cross protection against streptococcus iniae and vibrio anguillarum in japanese flounder.streptococcosis and vibriosis caused by streptococcus iniae and vibrio anguillarum respectively have affected fish culture industries around the world. previous studies have indicated that the s. iniae antigen sia10 and the v. anguillarum outer membrane protein ompu, when used as dna vaccines, induce protection in turbot (scophthalmus maximus) and asian seabass (lates calcarifer) respectively. in this study, with an effort to develop effective vaccines against s. iniae and v. anguillarum, we con ...201222480661
cpg odn mimicking cpg rich region of myxosporean myxobolus supamattayai stimulates innate immunity in asian sea bass (lates calcarifer) and defense against streptococcus iniae.oligodeoxynucleotides (odns) containing unmethylated cytosine-phosphate-guanine cpg dinucleotides within specific sequence contexts (cpg motifs) have been reported as pathogen-associated molecular patterns (pamps). its immunostimulatory effects have been demonstrated in diverse vertebrate models. cpg odn is typically found in bacterial or viral genome and recognized by a non-self recognition receptor toll-like receptor9 (tlr9). here, a new cpg odn 1013 which mimics sequence of ssu rdna of early ...201627629917
differential transcriptomic response in the spleen and head kidney following vaccination and infection of asian seabass with streptococcus iniae.vaccination is an important strategy in the protection of aquaculture species from major diseases. however, we still do not have a good understanding of the mechanisms underlying vaccine-induced disease resistance. this is further complicated by the presence of several lymphoid organs that play different roles when mounting an immune response. in this study, we attempt to elucidate some of these mechanisms using a microarray-based approach. asian seabass (lates calcarifer) were vaccinated agains ...201424992587
persistent infection of betanodavirus in a novel cell line derived from the brain tissue of barramundi lates order to obtain an in vitro system for studying the mechanism of persistent infection of fish nodavirus, a novel cell line (bb) was established from the brain tissue of a barramundi, lates calcarifer, which had survived viral nervous necrosis disease. the cell line has been subcultured > 100 times. the persistence of fish nodavirus designated as barramundi brain nervous necrosis virus (bbnnv) in the bb cells was demonstrated by: (1) the detection of the infectious virus in the culture superna ...200516060261
echium oil is better than rapeseed oil in improving the response of barramundi to a disease challenge.pathogen infection stimulates the fatty acid (fa) metabolism and the production of pro-inflammatory derivatives of fa. barramundi, lates calcarifer, was fed on a diet rich in preformed long-chain (⩾c20) polyunsaturated fatty acids (lc-pufa) from fish oil (fo), to compare with diets containing high levels of c18 precursors for lc-pufa - stearidonic (sda) and γ-linolenic acid (gla) - from echium plantagineum (eo), or rapeseed oil (ro) rich in α-linolenic acid (ala), but a poor source of lc-pufa an ...201323790934
molecular cloning and sequencing of australian black bream acanthopagrus butcheri and barramundi lates calcarifer fish growth hormone cdna using polymerase chain reaction.the complementary dna (cdna) encoding the preprotein growth hormone (pre-gh) from two australian marine fish species, namely acanthopagrus butcheri and lates calcarifer, have been isolated, cloned and sequenced. the sequences were amplified from reverse transcribed total rna of whole brains using polymerase chain reaction (pcr) and oligonucleotide primers corresponding to the 5' and 3' regions of pagrus major. use of pcr offers a rapid method of isolating fish gh cdna sequences for commercial an ...19911777674
a new epithelial cell line, hbf from caudal fin of endangered yellow catfish, horabagrus brachysoma (gunther, 1864).a new epithelial cell line, horabagrus brachysoma fin (hbf), was established from the caudal fin tissue of yellow catfish, h. brachysoma and characterized. this hbf cell line was maintained in leibovitz's-15 medium supplemented with 15 % fetal bovine serum (fbs) and subcultured more than 62 times over a period of 20 months. the hbf cell line consists predominantly of epithelial cells and is able to grow at temperatures between 20 and 35 °c with an optimum temperature of 28 °c. the growth rate of ...201625359669
cymothoa indica (crustacea, isopoda, cymothoidae) parasitizes the cultured larvae of the asian seabass lates calcarifer under laboratory conditions.parasitic disease in fishes is one of the most important factors limiting aquaculture production and its economic viability. cymothoa indica, a cymothoid isopod, is reported here for the first time parasitizing cultured larvae of the asian seabass lates calcarifer in india. fourteen-day-old l. calcarifer larvae of mean weight 8.73 +/- 0.03 mg were fed with wild zooplankton in the laboratory. on day 14 of rearing, larvae were found parasitized by cymothoids. infected larvae reached a mean (+/- se ...200516175971
azadirachta indica (neem) leaf dietary effects on the immunity response and disease resistance of asian seabass, lates calcarifer challenged with vibrio harveyi.the present study was aimed to address the possible evaluation of azadirachta indica (neem) leaf-supplemented diets on innate immune response in asian seabass, lates calcarifer fingerlings against vibrio harveyi infection. fish were fed for two weeks diets containing six graded levels of neem leaf at 0 g, 1 g, 2 g, 3 g, 4 g and 5 g per kg feed. fish fed neem leaf-supplemented diet displayed significant differences (p < 0.05) in weight gain, specific growth rate (sgr) and feed conversion ratio (f ...201323178500
characterization of the lect2 gene and its associations with resistance to the big belly disease in asian seabass.leukocyte cell-derived chemotaxin-2 (lect2) is an important protein of the innate immune system for the defense against bacterial infection. we cloned and characterized the lect2 gene from asian seabass (lates calcarifer). its complete cdna consisted of an open reading frame of 459 bp encoding a protein of 152 amino acids. the genomic dna sequence of this gene consists of four exons and three introns. quantitative real-time pcr revealed that the lect2 gene was expressed predominantly in liver wh ...201424486631
development and validation of a radioimmunoassay for fish insulin-like growth factor i (igf-i) and the effect of aquaculture related stressors on circulating igf-i levels.this paper describes the development and validation of a commercially available radioimmunoassay (ria) for the detection of fish insulin-like growth factor-i (igf-i). the assay was developed using recombinant barramundi igf-i as antigen and recombinant tuna igf-i as radiolabelled tracer and standard. assay sensitivity was 0.15 ng/ml, inter-assay variation was 16% (n = 9) and intra-assay variation was 3% (n = 10). cross reactivity of less than 0.01% was found with salmon insulin, salmon igf-ii an ...200414723878
fishmicrosat: a microsatellite database of commercially important fishes and shellfishes of the indian subcontinent.microsatellite dna is one of many powerful genetic markers used for the construction of genetic linkage maps and the study of population genetics. the biological databases in public domain hold vast numbers of microsatellite sequences for many organisms including fishes. the microsatellite data available in these data sources were extracted and managed into a database that facilitates sequences analysis and browsing relevant information. the system also helps to design primer sequences for flank ...201324047532
genetic and antigenic analysis of betanodaviruses isolated from aquatic organisms in taiwan.viral nervous necrosis (vnn) is a worldwide disease among marine fishes. in taiwan, nnn disease was first identified in 2 species of hatchery-reared grouper, epinephelus fuscogutatus and e. akaaya in 1994. since then, increasing mortalities have occurred among groupers epinephelus spp., and also among european eels anguilla anguilla l., yellow-wax pompano trachinotus falcatus, firespot snapper lutaanus erythropterus b., barramundi lates calcarifer, cobias rachycentron canadum, humpback groupers ...200313677508
effects of herbal essential oils used to extend the shelf life of freshwater-reared asian sea bass fish (lates calcarifer).sensory and microbiological characteristics of asian sea bass (lates calcarifer) fish raised in a freshwater pond were evaluated during cold storage for 33 days. whole fish (averaging 400 g each) were stored in a cold storage room at 0 to 2 degrees c. essential oils of herbs--thyme (thymus vulgaris) and oregano (origanum vulgare)--added at 0.05% (vol/vol) were used as preservatives. on the basis of the sensory test results as well as the bacteriological tests, the addition of oregano and thyme e ...200312636293
insulin-like growth factor mrna in barramundi (lates calcarifer): alternative splicing and nonresponsiveness to growth hormone.barramundi (lates calcarifer) is a teleost of the superorder acanthopterygii. barramundi igf-i cdna was cloned and the distribution of alternative transcripts in various barramundi tissues was investigated using rt-pcr and rpa. it was demonstrated that in barramundi tissues, igf-i mrnas were represented by two transcripts corresponding to the reported salmonid ea-2 and ea-4. the acute effect of gh on hepatic igf-i mrna levels was investigated. seven hours after intraperitonal administration of e ...199910495884
analysis of stress-responsive transcriptome in the intestine of asian seabass (lates calcarifer) using rna-seq.identification of differentially expressed genes (degs) and regulated pathways in response to stressors using a whole-genome approach is critical to understanding the mechanisms underlying stress responses. we challenged asian seabass with lipopolysaccharide (lps), vibrio harveyi, high salinity and fasting, and sequenced six cdna libraries of intestine samples using roche 454 rna-seq. over 1 million reads (average size: 516 bp) were obtained. the de novo assembly obtained 83 911 unisequences wit ...201323761194
a stochastic frontier analysis of technical efficiency of fish cage culture in peninsular malaysia.cage culture plays an important role in achieving higher output and generating more export earnings in malaysia. however, the cost of fingerlings, feed and labour have increased substantially for cage culture in the coastal areas in peninsular malaysia. this paper uses farm level data gathered from manjung, perak and kota tinggi, johor to investigate the technical efficiency of brackish water fish cage culture using the stochastic frontier approach. the technical efficiency was estimated and spe ...201627478744
experimental methods in aquatic respirometry: the importance of mixing devices and accounting for background light of an increasing trend in fish biology towards using static respirometry techniques without the inclusion of a mixing mechanism and without accurately accounting for the influence of microbial (background) respiration, this paper quantifies the effect of these approaches on the oxygen consumption rates (ṁo2 ) measured from juvenile barramundi lates calcarifer (mean ± s.e. mass = 20·31 ± 0·81 g) and adult spiny chromis damselfish acanthochromis polyacanthus (22·03 ± 2·53 g). background r ...201626768972
production, in vitro characterisation, in vivo clearance, and tissue localisation of recombinant barramundi (lates calcarifer) insulin-like growth factor ii.recombinant barramundi insulin-like growth-factor-ii (bigf-ii) has been produced in escherichia coli after modification of an expression plasmid that coded for a chicken igf-ii fusion protein. the bigf-ii fusion protein, deposited in bacterial inclusion bodies, was dissolved under reducing conditions, desalted, and refolded. the protein was then released from the fusion protein by cleavage with subtilisin bpn'. finally the protein was purified to homogeneity with a number of hplc steps. in vitro ...200111551116
control of parasites in cultured marine finfishes in southeast asia--an overview.mariculture in southeast asia began in the 1970s and expanded rapidly during the 1980s, with the commercial hatchery production of the seabass lates calcarifer. other important cultured species were epinephelus coioides, epinephelus malabaricus, lutjanus johni, and lutjanus argentimaculatus. intensification in the polyculture of these species and the large-scale international movement of fingerlings or juveniles, as well as the rapid expansion and concentration of fish farms, have caused severe ...19979394188
nodavirus infections in israeli mariculture.viral encephalopathy and retinopathy (ver) infections were diagnosed in five fish species: epinephelus aeneus, dicentrarchus labrax, sciaenops ocellatus, lates calcarifer and mugil cephalus cultured on both the red sea and mediterranean coasts of israel during 1998-2002. spongiform vacuolation of nervous tissue was observed in histological sections of all examined species. with transmission electron microscopy, paracrystalline arrays and pieces of membrane-associated non-enveloped virions measur ...200415291788
anti-immunoglobulin antisera used in an elisa to detect antibodies in barramundi lates calcarifer to cryptocaryon irritans.immunoglobulins (ig) in serum from barramundi vaccinated with bovine serum albumin (bsa) were purified by ammonium sulphate precipitation and affinity chromatography using bsa as the ligand. the bsa-binding activity of eluted putative ig fractions was assessed by enzyme-linked immunosorbent assay (elisa) before being pooled and characterised by sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page). double affinity purification did not improve the purity of the ig preparation comp ...199910349549
influence of temperature and host species on the development of cryptocaryon irritans.the course of infection of the parasitic ciliate cryptocaryon irritans was followed on lates calcarifer and macquaria novemaculeata at 20 and 25 c. the parasite was originally isolated from locally caught acanthopagrus australis. at 20 c trophonts stayed on the fish longer, tomonts took longer to excyst, and the resulting theronts were larger than at 25 c. on l. calcarifer at 20 c, trophonts grew slowly at first but eventually became significantly larger (mean tomont diameter 466 x 400 microns) ...19968627500
infection of barramundi lates calcarifer with streptococcus iniae: effects of different routes of exposure.the use of various challenge techniques has allowed the formation of a hypothesis for the mode of infection of streptococcus iniae in barramundi. a bacterial dose of 1 x 10(3) colony forming units (cfu), corresponding to the ld50, delivered orally to barramundi could initiate the sub-acute form of the disease observed at the farms. the acute form of the disease could be initiated through bath exposure to the pathogen. s. iniae was equally as infective in freshwater, saltwater or when fish were s ...200212553448
iron bioavailability in larvae yellow snapper (lutjanus argentiventris): cloning and expression analysis of ferritin-h.ferritin is a major intracellular iron storage protein in higher vertebrates and plays an important role in iron metabolism. in this study, ferritin h subunit was cloned from the larvae of yellow snapper, lutjanus argentiventris, by rapid amplification of cdna ends (race) following in silico transcriptome analysis. the full-length cdnas of the lafeh was 1231 bp in length encoding 177 amino acids with a predicted molecular mass (mw) about 20.82 kda and theoretical isoelectric point (pi) of 5.79. ...201424561126
effect of myoglobin from eastern little tuna muscle on lipid oxidation of washed asian seabass mince at different ph conditions.the effect of ph (6.0, 6.5, and 7.0) on lipid oxidation in washed asian seabass (lates calcarifer) mince mediated by oxymyoglobin from the dark muscle of little eastern tuna (euthynnus affinis) during 8 d of refrigerated storage was studied. metmyoglobin formation and discoloration increased with increasing storage time and the changes were more pronounced at lower ph. the highest lipid oxidation and off-odor development were observed when myoglobin was incorporated in washed mince at ph 6.0. at ...201121535742
streptococcus iniae, a bacterial infection in barramundi lates calcarifer.the cause of ongoing mortality in barramundi lates calcarifer (bloch) in seawater culture was identified as streptococcus iniae by biochemical and physiological tests. this is the first published record of this bacterial species in australia and the first confirmed report of s. iniae causing mortality in barramundi. the bacterium was highly pathogenic for barramundi when challenged by bath exposure. the pathogen was found to have a ld50 of 2.5 x 10(5) and 3.2 x 10(4) colony-forming units at 48 h ...199910401583
diplectanids infesting the gills of the barramundi lates calcarifer (bloch) (perciformes: centropomidae), with the proposal of laticola n. g. (monogenoidea: diplectanidae).four species of the monogenoidea, laticola lingaoensis n. sp., l. latesi (tripathi, 1957) n. comb. [previously pseudorhabdosynochus latesi (tripathi, 1957) kritsky & beverley-burton, 1986], l. paralatesi (nagibina, 1976) n. comb. [previously diplectanum paralatesi nagibina, 1976] and diplectanum penangi liang & leong, 1991, are reported from the gills of lates calcarifer (centropomidae) from the south china sea (new geographical records for l. latesi and d. penangi). collections from off bathurs ...200616718593
a new blood fluke, parasanguinicola vastispina gen. nov., sp. nov. (trematoda: sanguinicolidae), from sea bass lates calcarifer (centropomidae) cultured in malaysia.a new sanguinicolid blood fluke, parasanguinicola vastispina, is described from sea bass lates calcarifer cultured in malaysia. it is distinguished by its massive armature and widely spaced genital pores, the female pore being pre-ovarian. p. vastispina inhabits the branchial arteries, dorsal aorta, mesenteric venules and renal artery of its host. no pathological effect was observed in infected fish.19957624295
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