| cloning and comparative sequence analysis of tp53 in xiphophorus fish hybrid melanoma models. | we have cloned and sequenced the p53-encoding cdna of green swordtail (x. helleri) and southern platyfish (x. maculatus). these two fish species are often used to produce hybrids that develop melanomas after genetic crossing. computer translation of derived cdna sequences revealed that p53 polypeptides from these two species are virtually identical, exhibiting only two conservative amino acid substitutions. tp53 mrna expression was detected in virtually all tissues tested. comparison of these fi ... | 1998 | 9661661 |
| parasitic infections in live freshwater tropical fishes imported to korea. | we examined 15 species of ornamental tropical fishes originating from southeast asia to determine the cause of losses among 8 fish farms in korea. a total of 351 individuals belonging to 5 different families (1 species of characidae, 6 of cichlidae, 3 of cyprinidae, 1 of heleostomatidae, and 4 of poecilidae) were collected for the purpose of detecting metazoan and protozoan parasites. parasites were fixed and stained using routine methods, and identified. we found 3 ciliates, 2 monogeneans, 1 ne ... | 2002 | 12542094 |
| using a new inbred fish model and cultured fish tissue cells to study aeromonas hydrophila and yersinia ruckeri pathogenesis. | an inbred strain of the southern platyfish, xiphophorus maculatus, was used as a host for aeromonas hydrophila and yersinia ruckeri infections. the infections were initiated by holding the platyfish in inoculation baths containing dilutions of virulent a. hydrophila or y. ruckeri strains. inoculating the platyfish in this manner resulted in a dose-dependent mortality over a range of bacterial input from 10(5) to 10(8) a. hydrophila and 10(6) to 10(8) y. ruckeri/ml. clinical manifestations of a. ... | 1996 | 8722100 |
| assignment of the tp53 orthologue to a new linkage group (lg xiv) in fish of the genus xiphophorus (teleostei: poeciliidae). | using a p53 encoding cdna fragment of rainbow trout (oncorhynchus mykiss) as probe, a lambda clone from a platyfish (xiphophorus maculatus) genomic library was isolated. dna sequencing of the insert from this clone revealed that it contained the highly conserved domains iv and v of the p53 polypeptide. to map the xiphophorus p53 gene, joint segregation analysis of the inheritance of a psti-generated dna restriction fragment length polymorphism (rflp) and the inheritance of 36 polymorphic protein ... | 1996 | 8640724 |
| a zfy-like sequence in fish, with comments on the evolution of the zfy family of genes in vertebrates. | zfy-like genes have been observed in a variety of vertebrate species. although originally implicated as the primary testis-determining gene in humans and other placental mammals, more recent evidence indicates a role(s) outside that of testis determination. in this study, dna from five species of fish, carasius auratus, rivulus marmoratus, xiphophorus maculatus, x. milleri, and x. nigrensis was subjected to southern blot analysis using a pcr-amplified fragment of mouse zfy-like sequence as a pro ... | 1995 | 8595050 |
| an ovarian regression syndrome in the platyfish, xiphophorus maculatus. | the highly inbred coatzacoalcos (cp) strain of the platyfish, xiphophorus maculatus, was noted for a high percentage of infertile females (xx). the ovaries of approximately one-quarter of all females regress. the time of gonadal atrophy varied from before sexual maturation up to 11 months of age. the gonadotropic zone of the pituitary was hypertrophied in regressed females. transplants of immature testes and ovarian tissue into the caudal musculature of regressed females and the subsequent matur ... | 1985 | 4038733 |
| animal model for ultraviolet radiation-induced melanoma: platyfish-swordtail hybrid. | sunlight exposure is strongly indicated as one of the important etiologic agents in human cutaneous malignant melanoma. however, because of the absence of good animal models, it has not been possible to estimate the wavelengths or wavelength regions involved. we have developed a useful animal model from crosses and backcrosses of platyfish (xiphophorus maculatus) and swordtails (xiphophorus helleri). two strains of these fish are susceptible to invasive melanoma induction by exposure to filtered ... | 1989 | 2813430 |
| genetic and molecular approach for breeding and use of laboratory fish for the detection of agents with carcinogenic and/or promoting activity. | xiphophorine fish from wild populations are insusceptible to develop neoplasia. in contrast, certain backcrosses of xiphophorus maculatus (platyfish) with xiphophorus helleri (swordtail) as the recurrent parent, produce offspring that develop neoplasia in a mendelian fashion. we concentrated our research on melanoma. the starting signal for the development of melanoma comes from an accessory v-erbb-related oncogene, x-erbb*, which is highly homologous to the human egf receptor gene, and is part ... | 1991 | 1878604 |
| detection of mycobacteria in aquarium fish in slovenia by culture and molecular methods. | thirty-five aquarium fish were investigated for the presence of mycobacteria by culture and molecular methods. the following species were examined: goldfish carassius auratus auratus, guppy poecilia reticulata, 4 three-spot gourami trichogaster trichopterus, dwarf gourami colisa lalia, siamese fighting fish betta splendens, freshwater angelfish pterophyllum scalare, african cichlid fish cichlidae spp., cichlid fish microgeophagus altispinosus, cichlid fish pseudotropheus lombardoi, blue streak h ... | 2005 | 15900685 |
| real-time pcr-based method for the estimation of genome sizes. | the fast and reliable estimation of the genome sizes of various species would allow for a systematic analysis of many organisms and could reveal insights into evolutionary processes. many methods for the estimation of genome sizes have already been described. the classical methods are based on the determination of the phosphate content in the dna backbone of total dna isolated from a defined number of cells or on reassociation kinetics of high molecular weight genomic dna (c(0)t assay). more rec ... | 2003 | 12736322 |
| cloning and analysis of a foxo transcription factor from xiphophorus. | melanoma development in the fish xiphophorus is determined, at least in part, by overexpression and activation of the xmrk-2 oncogene, which triggers a variety of signal transduction pathways resulting in altered cell cycle control. we have begun analysing transcription factors which may link xmrk-2 with regulation of cell proliferation or apoptosis. towards this end, we have cloned an fkhr (foxo sub-family) homolog from xiphophorus maculatus. the isolated clone is a 2.7 kb cdna encoding a predi ... | 2003 | 12527194 |
| endocytosis of ferritin and hemoglobin by the trabecular endocardium in swordtail, xiphophorus helleri l. and platy, xiphophorus maculatus l. (poecilidae: teleostei). | the cardiac atrium and ventricle of swordtail, xiphophorus helleri l. and platy, xiphophorus maculatus l., are spongious, consisting of muscle trabeculae covered by endocardial cells. the cardiac trabecular endocardium is able to take up and store large amounts of horse-spleen ferritin and bovine hemoglobin from the blood stream. no such uptake was registered in endocardial cells lining the cardiac valves, atrio-ventricular junction and ventriculo-bulbar junction. the trabecular endocardium in t ... | 2001 | 11396795 |
| non-ltr retrotransposons encoding a restriction enzyme-like endonuclease in vertebrates. | all autonomous non-long terminal repeat (non-ltr) retrotransposons reported to date in vertebrates encode an apurinic/apyrimidinic endonuclease-like enzyme necessary for target sequence cleavage and subsequent target-primed reverse transcription. we describe here vertebrate non-ltr retrotransposons encoding another type of endonuclease more related to type iis restriction enzymes. such retrotransposons have been detected until now only in trypanosomes, nematodes, and arthropods. the retrotranspo ... | 2001 | 11343131 |
| jule from the fish xiphophorus is the first complete vertebrate ty3/gypsy retrotransposon from the mag family. | jule is the second complete long-terminal-repeat (ltr) ty3/gypsy retrotransposon identified to date in vertebrates. jule, first isolated from the poeciliid fish xiphophorus maculatus, is 4.8 kb in length, is flanked by two 202-bp ltrs, and encodes gag (structural core protein) and pol (protease, reverse transcriptase, rnase h, and integrase, in that order) but no envelope. there are three to four copies of jule per haploid genome in x. maculatus. two of them are located in a subtelomeric region ... | 2001 | 11158369 |
| post-embryonic development of camallanus cotti (nematoda: camallanidae), with emphasis on growth of some taxonomically important somatic characters. | in this paper, the quantitative post-embryonic development of the asian freshwater fish nematode camallanus cotti fujita, 1927, is described. larval and adult morphometrics were obtained by following the parasite's life cycle experimentally using copepods macrocyclops albidus (jurine) as intermediate host and guppies poecilia reticulata (peters), southern platyfish xiphophorus maculatus (günther) and paradise fish macropodus opercularis (l.) as definitive host. additionally, adult worms were obt ... | 2002 | 12418817 |
| characterization of four flavobacterium columnare (flexibacter columnaris) strains isolated from tropical fish. | four flavobacterium columnare strains (ajs 1-4) were isolated from black mollies (poecilia sphenops) and platies (xiphophorus maculatus), showing white spots on the back, head and skin ulcers. the isolates developed characteristic rhizoid yellow pigmented colonies on shieh agar and typical growth in shieh broth. they were gram-negative, filamentous bacteria exhibiting flexing movements. when compared to f. columnare strains isolated from temperate fish, it was noted that the four strains origina ... | 1998 | 9659690 |
| molecular analysis of ercc2 mutations in the repair deficient hamster mutants uvl-1 and v-h1. | the cdna sequence of the chinese hamster ercc2 nucleotide excision repair and transcription gene from the uvl-1 chinese hamster ovary (cho) mutant cell line and the v-h1 chinese hamster v79 mutant line was analyzed. ercc2 encodes a presumed atp-dependent dna helicase and is single copy in cho lines due to the structural hemizygosity of chromosome 9. both uvl-1 and v-h1 have intermediate levels of (6-4) photoproduct repair but are as highly uv sensitive as the group 2 mutants that have no detecta ... | 1997 | 9372848 |
| characterization and mapping of the xiphophorus maculatus (teleostei: poeciliidae) rps15 gene. | we have determined the nucleotide sequence and gene map location of the xiphophorus maculatus homologue of rps15 (ribosomal protein s15, alias rig). the xiphophorus rps15 cdna encodes 145 amino acids, which show 94% identity compared to deduced mammalian and avian rps15 amino acid sequences. at the nucleotide level, 84% sequence identity is maintained between the fish and human gene, while homologous amphibian and avian sequences show about 80% nucleotide identity compared to the xiphophorus seq ... | 1996 | 9040780 |
| parasitic infections in freshwater ornamental fish in sri lanka. | a total of 1520 ornamental fish of 13 species from 26 export farms in sri lanka were collected between october 1999 and march 2000 and examined for parasites. fish species examined were guppy poecilia reticulata, goldfish carassius auratus, platy xiphophorus maculatus, molly poecilia sphenops, angel pterophyllum scalare, swordtail xiphophorus helleri, tetras hyphessobrycon species, barbs capeota and puntius spp., gourami colisa sp., carp cyprinus carpio, fighters betta spelendens and others (bra ... | 2003 | 12747641 |
| expression of pejerrey gonadotropin-releasing hormone in three orders of fish. | molecular variants of gnrh were characterized by reverse-phase, high-performance liquid chromatography from brain extracts of fish in three different orders: synbranchiformes (swamp eel [synbranchus marmoratus]), cyprinidontiformes (platyfish [xiphophorus maculatus] and green swordtail [x. helleri]), and atheriniformes (patagonia pejerrey [odontesthes hatchery]). also, pituitary gland extracts from the pejerrey o. bonariensis (atheriniformes) were characterized. eluted fractions were tested in r ... | 2002 | 12444064 |
| transformation-associated recombination (tar) cloning of tumor-inducing xmrk2 gene from xiphophorus maculatus. | we modified the tar methodology of yac clone construction for application to fish genomic dna isolated from xiphophorus maculatus. yac libraries were developed using the xir1 repeat sequence as the recombinational hook. construction of these libraries demonstrates that xiphophorus dna sequences can function as hooks in the yeast recombination system and that x. maculatus genomic dna contains sequences that provide origin of replication function in yeast. by screening a subset of xiphophorus yac ... | 2001 | 14961313 |
| distribution of variant forms of immunoreactive gonadotropin-releasing hormone and beta-gonadotropins i and ii in the platyfish, xiphophorus maculatus, from birth to sexual maturity. | immunoreactive (ir) lamprey (l), mammalian (m), chicken ii (chii), and salmon (s) gonadotropin-releasing hormone (gnrh) and ir-coho salmon beta-gonadotropins i and ii (gth i and gth ii) have been localized in the brain and pituitary gland of xiphophorus maculatus, the platyfish, at various stages of development from birth to sexual maturity. ir-gth i was found in the pituitary gland at all stages examined; ir-gth ii was found only in animals in the pubertal and mature stages. ir-sgnrh was seen o ... | 1994 | 8045363 |
| uptake of foreign ferritin in platy xiphophorus maculatus (poeciliidae: teleostei). | the ability and capacity of various tissues in platy xiphophorus maculatus l. to take up horse-spleen ferritin injected into the blood stream are described. ferritin was injected intraperioneally, and the cellular uptake was demonstrated as prussian blue precipitations in tissues treated with acid ferrocyanide solutions. ferritin was detected within the heart endocardial cells and macrophages in the trunk kidney and spleen, 1/4 h after the injection, i.e. foreign ferritin was taken up very rapid ... | 2002 | 12465881 |
| identification and mapping of two divergent, unlinked major histocompatibility complex class ii b genes in xiphophorus fishes. | we have isolated two major histocompatibility complex (mhc) class ii b genes from the inbred fish strain xiphophorus maculatus jp 163 a. we mapped one of these genes, designated here as dxb, to linkage group iii, linked to a malic enzyme locus, also syntenic with human and mouse mhc. comparison of genomic and cdna clones shows the gene consists of six exons and five introns. the encoded beta1 domain has three amino acids deleted and a cytoplasmic tail nine amino acids longer than in other teleos ... | 1998 | 9691047 |
| cloning of the dmrt1 gene of xiphophorus maculatus: dmy/dmrt1y is not the master sex-determining gene in the platyfish. | in contrast to the situation observed in mammals and birds, a switching between different sex determination systems frequently occurred during the evolution of the teleost fish lineage. this might be due to a frequent turnover of sex-determining signals at the top of the sex determination cascade (master sex-determining genes in the case of genetic sex determination). alternatively, different variations of a same master gene might decide the sex of individuals in different sex determination syst ... | 2003 | 14604792 |
| the demonstration of neurophysin and arginine vasotocin by immunocytochemical methods in the brain and pituitary gland of the platyfish, xiphophorus maculatus. | | 1980 | 7353780 |
| cross-reactivity between human and fish pituitary hormones as demonstrated by immunocytochemistry. | in this communication we describe the immunocytochemical cross-reactivity between antisera to various human pituitary hormones and specific hormone producing cell types in the pituitary gland of sexually mature male platyfish (xiphophorus maculatus). antisera to human pituitary hormones cross-reacted either with cells known to produce corresponding hormones (or hormone subunits) in the platyfish (e.g., acth, prolactin, tsh beta, lh alpha, fsh alpha, tsh alpha) or with no pituitary cells at all ( ... | 1981 | 7307052 |
| generation of poly- and mono-clonal antibodies against trout fibronectin (fn) and their use in fn immunodetection in teleost fishes. | immunization of rats with gelatin-affinity column purified fibronectin (fn) from rainbow trout (oncorhynchus mykiss) plasma produced a polyclonal antiserum that reacts specifically with fn in immunoblotted protein extracts and cultured cells, not only from trout but also from swordtails (xiphophorus helleri). most importantly, this antiserum specifically stains fn-containing structures in sections from embryos, as well as skin and dorsal fin of swordtails and platyfish (xiphophorus maculatus), a ... | 1994 | 7893474 |
| isolation and characterization of a retrovirus from the fish genus xiphophorus. | a cell line (bst) established from neoplastic embryonal tissues of the platyfish (xiphophorus maculatus) released spontaneously retrovirus-like particles. the particles have a buoyant density of 1.16 g/ml, a mean diameter of 100 nm and the morphology of immature retroviruses. the particle-associated proteins p70, p65, and p28 react with an antiserum directed against the major internal feline leukemia virus structural protein p27. the particles are associated with a reverse transcriptase. the pur ... | 1992 | 1374984 |
| characterization of the xiphophorus fish (teleostei: poeciliidae) ercc2/xpd locus. | we have cloned and sequenced the ercc2/xpd locus of xiphophorus maculatus. the human ercc2/xpd gene is a nucleotide excision repair gene presumed to encode an atp-dependent dna helicase. the fish ercc2/xpd gene is represented on 14.5 kb of genomic dna and is composed of 23 exons. within the coding regions, the overall nucleotide identity is 74% compared to the human cdna. of 760 amino acids compared between human and fish sequences, 127 differences are observed. of these differences, 48 residues ... | 1995 | 7782088 |
| two novel elements (cfg1 and pyg1) of mag lineage of ty3/gypsy retrotransposons from zhikong scallop (chlamys farreri) and japanese scallop (patinopecten yessoensis). | two novel elements (cfg1 and pyg1) of mag lineage of ty3/gypsy retrotransposons were cloned from zhikong scallop (chlamys farreri) and japanese scallop (patinopecten yessoensis). the total length of the cfg1 element is 4826 bp, including 5'-ltr (192 bp), the entire orf (4047 bp) and 3'-ltr (189 bp). the entire orfs of both cfg1 and pyg1 elements are composed of 1348 aa and do not have any frameshifts. their closest relative is jule element from the poeciliid fish (xiphophorus maculatus). on aver ... | 2008 | 17694394 |
| multi-step genetic regulation of oncogene expression in fish hereditary melanoma. | melanoma occurring spontaneously in xiphophorus fish hybrids is a model system in which involvement of cellular oncogenes and multi-step regulation of their expression have been identified by classical genetics. the macromelanophore gene in platyfish (xiphophorus maculatus) is a sex-linked codominant gene which determines the black spot patterns of macromelanophores in the skin. the macromelanophore locus includes a cellular oncogene which potentially induces neoplasms of the pigment cells. expr ... | 1983 | 6354817 |
| characterization and purification of flap endonuclease-1 (xifen-1) from xiphophorus maculatus. | the cloning, gene structure, and expression of flap endonuclease-1 (xifen1) from xiphophorus maculates are presented. the xifen1 gene structure was found to include 8 exons and 7 introns. the xiphophorus fen1 cdna sequence contained an open reading frame that encoded a 380 amino acid protein with a predicted mass of 43 kda. the intact fen1 cdna was subcloned into a bacterial expression vector (pet101-xifen1ct) and recombinant xifen1 enzyme purified from e. colicell extracts. the pet101-xifen1ct ... | 2004 | 18248237 |
| cloning of juna and junb and comparison of mrna expression levels in two xiphophorus melanoma models. | the cloning and mrna expression analysis of xiphophorus maculatus juna and junb proto-oncogenes (designated x-juna and x-junb, respectively) is described. in mammals, juna and junb proteins make up the activator protein-1 (ap-1) transcription factor with related fos proteins. the deduced amino acid sequences of x-juna and x-junb exhibit moderate degrees of similarity when compared to their human homologues, while the regions considered functionally critical, namely, the transactivation domains, ... | 2006 | 18248246 |
| molecular analysis of the sex-determining region of the platyfish xiphophorus maculatus. | due to the presence of genetically well-defined sex chromosomes, with a relatively restricted sex-determination region containing markers identified at the molecular level, the platyfish xiphophorus maculatus is one of the best models for the positional cloning of a master sex-determining gene in fish. both male and female heterogametes and three different types of sex chromosomes have been described in the platyfish, with several loci involved in pigmentation, melanoma formation, and sexual mat ... | 2006 | 18377211 |
| a structural model of the extracellular domain of the oncogenic egfr variant xmrk. | the epidermal growth factor receptor (egfr) variant xmrk of the mexican fish xiphophorus is one of the first oncogenes described. its overexpression in pigment cells of the skin occurs after certain repeated cross-breedings between the platyfish xiphophorus maculatus and the swordtail xiphophorus hellerii. xmrk overexpression results in melanoma with a high malignant potential. the xiphophorus melanoma model provides a valuable tool for the understanding of melanoma development in general. the x ... | 2006 | 18377216 |
| heat shock proteins protect platyfish (xiphophorus maculatus) from yersinia ruckeri induced mortality. | the significant disadvantages accompanied with the use of antibiotics in aquaculture, emphasize the need for developing alternative disease control strategies, like novel vaccine approaches and immunostimulating measures. several studies have already pointed out the ability of heat shock proteins (hsps) to modulate innate and adaptive immune responses, what makes them potent candidates for the development of a new disease prevention method. in this study, the use of self and non-self heat shock ... | 2010 | 19751832 |
| the vertebrate makorin ubiquitin ligase gene family has been shaped by large-scale duplication and retroposition from an ancestral gonad-specific, maternal-effect gene. | members of the makorin (mkrn) gene family encode ring/c3h zinc finger proteins with u3 ubiquitin ligase activity. although these proteins have been described in a variety of eukaryotes such as plants, fungi, invertebrates and vertebrates including human, almost nothing is known about their structural and functional evolution. | 2010 | 21172006 |
| characterization of telomeres and telomerase expression in xiphophorus. | research investigating telomere lengths and telomerase expression in vertebrates has progressively become important due to the association of these two biological endpoints with cellular aging and cancer in humans. studies that rely upon the traditional use of laboratory mice have been faced with limitations largely due to inbred mice possessing large telomeres and ubiquitous expression of telomerase. recently, a number of small fish species have been shown to provide potentially informative mod ... | 2011 | 21619941 |
| the immunocytochemical localization of gonadotropin, its subunits, and thyrotropin in the teleost, xiphophorus maculatus. | | 1984 | 391639 |
| gene structure, purification and characterization of dna polymerase beta from xiphophorus maculatus. | cloning of the xiphophorus maculatus polbeta gene and overexpression of the recombinant polbeta protein has been performed. the organization of the xiphpolbeta introns and exons, including intron-exon boundaries, have been assigned and were found to be similar to that for human polbeta with identical exon sizes except for exon xii coding for an additional two amino acid residues in xiphophorus. the cdna sequence encoding the 337-amino acid x. maculatus dna polymerase beta (polbeta) protein was s ... | 2004 | 15533789 |
| detection of dwarf gourami iridovirus (infectious spleen and kidney necrosis virus) in populations of ornamental fish prior to and after importation into australia, with the first evidence of infection in domestically farmed platy (xiphophorus maculatus). | the movement of ornamental fish through international trade is a major factor for the transboundary spread of pathogens. in australia, ornamental fish which may carry dwarf gourami iridovirus (dgiv), a strain of infectious spleen and kidney necrosis virus (isknv), have been identified as a biosecurity risk despite relatively stringent import quarantine measures being applied. in order to gain knowledge of the potential for dgiv to enter australia, imported ornamental fish were sampled prior to e ... | 2015 | 26452601 |
| melano-macrophage centres and endocytic cells in kidney and spleen of pearl gouramy and platyfish (anabantidae, poeciliidae: teleostei). | the structure and putative cellular content of melano-macrophage centres (mmcs) and single macrophages in kidney and spleen of two teleosts, pearl gouramy trichogaster leeri (bleeker) and platyfish xiphophorus maculatus (günther), are described and compared. the mmcs were confined to the haemopoetic tissue in both species, and were markedly more prominent in pearl gouramy than in platyfish. they were rich in iron-compounds, probably haemosiderin, in the former species, whereas they contained mai ... | 2007 | 17113134 |
| evolution of melanocortin receptors in teleost fish: the melanocortin type 1 receptor. | the melanocortin type 1 receptor (mc1r) belongs to a family of g-protein-coupled receptors involved in various physiological processes in vertebrates. melanocortins, the mcr natural agonists, are pituitary peptide hormones including adrenocorticotropin and melanocyte-stimulating hormones. in mammals and birds, mc1r is involved in pigmentation and expressed in melanocytes and melanoma. activation of mc1r leads to eumelanin production as well as to proliferation and survival of melanocytes in the ... | 2007 | 17707598 |
| offspring production with cryopreserved sperm from a live-bearing fish xiphophorus maculatus and implications for female fecundity. | xiphophorus fishes are well-established models for biomedical research of spontaneous or induced tumors, and their use in research dates back to the 1930s. currently, 58 well-pedigreed lines exist among 24 xiphophorus species housed as live animals at the xiphophorus genetic stock center. the technique of sperm cryopreservation has been applied to preserve these valuable genetic resources, and production of offspring has been reported with cryopreserved sperm in two species (x. helleri and x. co ... | 2011 | 21616165 |
| ecotoxicological assessment of pyriproxyfen under environmentally realistic exposure conditions of integrated vector management for aedes aegypti control in brazil. | there is increasing concern to control aedes aegypti mosquito exposure in developing countries such as brazil. thus, integrated approaches using a combination of chemical, pyriproxyfen larvicide, and biological, xiphophorus maculatus, larvivorous fish species approaches are necessary and important to initiate more effective control against mosquito borne diseases. this study describes the toxicological effects of pyriproxyfen larvicide on the fish xiphophorus maculatus, the larvivorous fish spec ... | 2016 | 27458879 |
| toxicological assessment of spinosad: implications for integrated control of aedes aegypti using larvicides and larvivorous fish. | integration of larvivorous fish and biolarvicides at low concentrations to control of mosquito larvae in field situations may result in a safer and more effective tool. however, the usefulness of integrated approach depends upon survival and ecological fitness of fish employed. thus, the aim of this study was to examine the genotoxic effects of combining different sublethal concentrations of spinosad, a naturally occurring neurotoxic insecticide, with male adult poecilid larvivorous guppy (poeci ... | 2016 | 27294296 |
| on the structure of intestine and presence of endocytic cells in the lamina propria of platy and black tetra (teleostei). | the structure of the intestine in platy (xiphophorus maculatus) and black tetra (gymnocorymbus ternetzi) and the capability of cells within the intestinal wall to endocytose intraperitoneally injected horse-spleen ferritin, are described. the intestinal epithelial layer has about the same thickness in both species, but the width of the lamina propria and tunica muscularis in black tetra was only about 1/5 of that in platy. ferritin was taken up by numerous cells within the lamina propria through ... | 2005 | 15752653 |
| trichodina nobilis chen, 1963 and trichodina reticulata hirschmann et partsch, 1955 from ornamental freshwater fishes in brazil. | in the present work trichodina reticulata and t. nobilis (ciliophora: trichodinidae) are morphologically characterised from ornamental freshwater fish culture in the state of santa catarina, brazil. the prevalence of infection and a list of comparative measurements are discussed. we examined "southern platyfish" xiphophorus maculatus (n = 35), "goldfish" carassius auratus (n = 31), "guppy" poecilia reticulata (n = 20), "sailfin molly" poecilia latipinna (n = 6), "beta" betta splendens (n = 2) an ... | 2012 | 22735135 |
| on the ultrastructure of the ventricular endocardium and bulbar endothelium in 3 teleostean species. | the endocardium in the ventricle and the endothelium in the bulbus arteriosus are described in 3 phylogenetically distant teleostean species, betta splendens, pistella riddlei, and xiphophorus maculatus. the ventricular endocardial cells are attenuated and contain a number of bristle-coated vesicles (bcv). in the former 2 species these cells contain numerous ribosomes, a low number of moderately electron dense bodies (mdb) and a poorly developed agranular endoplasmic reticulum (aer). in the latt ... | 1982 | 7158794 |
| regulation of cdkn2a/b and retinoblastoma genes in xiphophorus melanoma. | xiphophorus interspecies hybrids provide several well-characterized genetic models of melanoma susceptibility. the xiphophorus cdkn2a/b gene, homologous to mammalian cdkn2a/b cyclin-dependent kinase inhibitors (p16 and p15), is a candidate tumor susceptibility gene in these models. using real-time pcr and western blot analysis, we analyzed expression of cdkn2a/b in spontaneous and uv-induced primary melanomas from individual backcross hybrid fish. we found that cdkn2a/b mrna is highly expressed ... | 2007 | 17011832 |
| expansion of the known host range of the microsporidium, pseudoloma neurophilia. | the microsporidium, pseudoloma neurophilia, is the most common infectious organism found in laboratory zebrafish colonies. many currently used zebrafish lines originally came from pet store fish, and the initial description of p. neurophilia came from zebrafish obtained from a retail pet store. however, as p. neurophilia has not been described from wild-caught zebrafish, whether p. neurophilia is a natural pathogen of zebrafish is an open question. the pooling of fish of different species in the ... | 2016 | 27182659 |
| nonmammalian models for sunlight carcinogenesis: genetic analysis of melanoma formation in xiphophorus hybrid fish. | genetic hybrids of xiphophorus fishes have been used for decades to study heritable melanoma formation. in these models, overexpression of pigmentation patterns from melanin-producing pigment cells can lead to genetically regulated melanoma formation in backcross hybrids. in the best studied of these models, the gordon-kosswig hybrid melanoma, tumors form spontaneously in all individuals of a subset of backcross hybrids between the platyfish xiphophorus maculatus jp 163 a and the swordtail speci ... | 1996 | 8806224 |
| molecular confirmation of infectious spleen and kidney necrosis virus (isknv) in farmed and imported ornamental fish in australia. | viruses of the genus megalocytivirus have not been detected in wild populations of fish in australia but circulate in imported ornamental fish. in 2012, detection of a megalocytivirus in healthy platys xiphophorus maculatus was reported from a farm in australia during surveillance testing as part of a research project undertaken at the university of sydney. confirmatory testing of the original samples at the aahl fish diseases laboratory verified the presence of an infectious spleen and kidney n ... | 2015 | 26480913 |
| first outbreak of an infection with infectious spleen and kidney necrosis virus (isknv) in ornamental fish in germany. | in 2014, infectious spleen and kidney necrosis virus (isknv), a member of the genus megalocytivirus, was detected for the first time in ornamental fish in germany. since 2013, angelfish pterophyllum spp. originating from colombia have experienced significant epizootics in a number of german retailers' facilities. the diseased fish showed symptoms such as increased ventilation, swollen gills, and ulcerations of the skin. in 2014, diseased angelfish p. altum and platys xiphophorus maculatus mainta ... | 2016 | 27225207 |
| recombinant expression and purification of the rna-binding larp6 proteins from fish genetic model organisms. | the rna-binding proteins that comprise the la-related protein (larp) superfamily have been implicated in a wide range of cellular functions, from trna maturation to regulation of protein synthesis. to more expansively characterize the biological function of the larp6 subfamily, we have recombinantly expressed the full-length larp6 proteins from two teleost fish, platyfish (xiphophorus maculatus) and zebrafish (danio rerio). the yields of the recombinant proteins were enhanced to >2 mg/l using a ... | 2017 | 28400296 |
| gridded genomic libraries of different chordate species: a reference library system for basic and comparative genetic studies of chordate genomes. | the use of genomic libraries maintained in arrayed format is becoming a more and more popular tool for the analysis of molecular evolution and comparative molecular development. being able to use already existing reference libraries considerably reduces the work load, and if results are made publicly available, it will facilitate in silica experiments in the future. here we describe the construction and preliminary characterization of six cosmid libraries of different chordate species, ciona int ... | 1998 | 9782092 |
| action of melanin-concentrating hormone (mch) on teleost chromatophores. | the in vitro effects of synthetic salmon melanin-concentrating hormone (mch) on chromatophores of four teleost species were studied. in the erythrophores of the platyfish (xiphophorus maculatus) and the swordtail (xiphophorus helleri), and in the xanthophores and amelanotic melanophores of the medaka (oryzias latipes), pigment aggregation took place in response to mch even in the absence of ca2+. in contrast to this, the leucophores of the medaka responded to mch by the pigment dispersion but on ... | 1986 | 3026881 |
| meiotic chromosomes and stages of sex chromosome evolution in fish: zebrafish, platyfish and guppy. | we describe sc complements and results from comparative genomic hybridization (cgh) on mitotic and meiotic chromosomes of the zebrafish danio rerio, the platyfish xiphophorus maculatus and the guppy poecilia reticulata. the three fish species represent basic steps of sex chromosome differentiation: (1) the zebrafish with an all-autosome karyotype; (2) the platyfish with genetically defined sex chromosomes but no differentiation between x and y visible in the sc or with cgh in meiotic and mitotic ... | 2001 | 11778689 |
| identification and characterization of a ltr retrotransposon from the genome of cyprinus carpio var. jian. | a ty3/gypsy-retrotransposon-type transposon was found in the genome of the jian carp (cyprinus carpio var. jian) in a previous study (unpublished), and was designated a jre retrotransposon (jian retrotransposon). the full-length jre retrotransposon is 5126 bp, which includes two long terminal repeats of 470 bp at the 5' end and 453 bp at the 3' end, and two open reading frames between them: 4203 bp encoding the group-specific antigen (gag) and polyprotein (pol). the pol gene has a typical ty3/gy ... | 2016 | 27178280 |
| cloning and expression of a novel neurotrophin, nt-7, from carp. | neurotrophins have been demonstrated to play important roles in the development and functioning of the nervous system. this family of proteins consists of four homologous members in mammals: ngf, bdnf, nt-3, and nt-4/5. a new member, called nt-6, was recently cloned from the platyfish xiphophorus maculatus. this protein shares closer structural relationship to ngf than the other neurotrophins, but contains a characteristic insertion of 22 amino acids that constituted the heparin-binding domain. ... | 1998 | 12546061 |
| a transcriptome derived female-specific marker from the invasive western mosquitofish (gambusia affinis). | sex-specific markers are a prerequisite for understanding reproductive biology, genetic factors involved in sex differences, mechanisms of sex determination, and ultimately the evolution of sex chromosomes. the western mosquitofish, gambusia affinis, may be considered a model species for sex-chromosome evolution, as it displays female heterogamety (zw/zz), and is also ecologically interesting as a worldwide invasive species. here, de novo rna-sequencing on the gonads of sexually mature g. affini ... | 2015 | 25707007 |
| comparison of de novo transcriptome assemblers and k-mer strategies using the killifish, fundulus heteroclitus. | de novo assembly of non-model organism's transcriptomes has recently been on the rise in concert with the number of de novo transcriptome assembly software programs. there is a knowledge gap as to what assembler software or k-mer strategy is best for construction of an optimal de novo assembly. additionally, there is a lack of consensus on which evaluation metrics should be used to assess the quality of de novo transcriptome assemblies. | 2016 | 27054874 |
| import of exotic and zoonotic trematodes (heterophyidae: centrocestus sp.) in xiphophorus maculatus: implications for ornamental fish import control in europe. | ornamental fish, xiphophorus maculatus, were imported from singapore to denmark for distribution to local aquarists. importers observed lethargic and erratic swimming patterns among fish and forwarded a total of 30 fish for pathological examination to a university diagnostic service. all fish were diagnosed infected with encysted centrocestus sp. metacercariae in gills (prevalence of 100% and mean intensity of 454.5 ± 161.9 parasites per fish). metacercariae were identified by morphological and ... | 2014 | 24827099 |
| a rad-tag genetic map for the platyfish (xiphophorus maculatus) reveals mechanisms of karyotype evolution among teleost fish. | mammalian genomes can vary substantially in haploid chromosome number even within a small taxon (e.g., 3-40 among deer alone); in contrast, teleost fish genomes are stable (24-25 in 58% of teleosts), but we do not yet understand the mechanisms that account for differences in karyotype stability. among perciform teleosts, platyfish (xiphophorus maculatus) and medaka (oryzias latipes) both have 24 chromosome pairs, but threespine stickleback (gasterosteus aculeatus) and green pufferfish (tetraodon ... | 2014 | 24700104 |
| molecular genetic response of xiphophorus maculatus-x. couchianus interspecies hybrid skin to uvb exposure. | the phenotypic and genetic similarities between xiphophorus and human melanoma render xiphophorus a useful animal model for studying the genetic basis of melanoma etiology. in the xiphophorus model, melanoma has been shown to be inducible by ultraviolet light (uvb) exposure among interspecies hybrids, but not in parental line fish similarly treated. this leads to questions of what genes are responsive to uvb exposure in the skin of the interspecies hybrids, as well as how parental alleles in hyb ... | 2015 | 26254713 |
| a microsatellite genetic linkage map for xiphophorus. | interspecies hybrids between distinct species of the genus xiphophorus are often used in varied research investigations to identify genomic regions associated with the inheritance of complex traits. there are 24 described xiphophorus species and a greater number of pedigreed strains; thus, the number of potential interspecies hybrid cross combinations is quite large. previously, select xiphophorus experimental crosses have been shown to exhibit differing characteristics between parental species ... | 2004 | 15454549 |