| cell-free synthesis of fish preproinsulin, and processing by heterologous mammalian microsomal membranes. | poly(a)-containing mrna isolated from the islets of langerhans obtained from two species of fish, angler fish (lophius americanus) and sea raven (hemitripterus americanus), stimulated protein synthesis 16-fold in a wheat germ cell-free system. characterization of the translation products by polyacrylamide gel electrophoresis in sodium dodecyl sulfate showed a major polypeptide weighing 11,500 daltons that was specifically precipitated by an antibody against angler fish insulin. partial sequence ... | 1977 | 325565 |
| primary structure and tissue distribution of anglerfish carboxypeptidase h. | most peptide hormones are synthesized as part of larger precursor proteins which must be processed after translation to generate bioactive peptides. this usually involves cleavage of the precursor by an endopeptidase at sites marked by basic amino acids, followed by removal of n- or c-terminal basic residues by the action of an aminopeptidase or carboxypeptidase. these processing events have been observed in a variety of species, from yeast to mammals. as part of an effort to characterize prohor ... | 1991 | 1778303 |
| kinetic analyses of peptidylglycine alpha-amidating monooxygenase from pancreatic islets. | peptidylglycine alpha-amidating monooxygenase (pam) plays an important role in the post-translational processing of bioactive neuropeptides by participating in c-terminal amidation. we have examined pam activity in the pancreatic islets of the anglerfish (af), lophius americanus. it was previously demonstrated that the cofactor requirements and ph optimum for the fish pam are essentially identical to pam obtained from other tissues and species. the present study was performed to examine the enzy ... | 1991 | 1916206 |
| localization and characterization of neuropeptide y-like peptides in the brain and islet organ of the anglerfish (lophius americanus). | results from a previous report demonstrate that more than one molecular form of neuropeptide y-like peptide may be present in the islet organ of the anglerfish (lophius americanus). most of the neuropeptide y-like immunoreactive material was anglerfish peptide yg, which is expressed in a subset of islet cells, whereas an additional neuropeptide y-like peptide(s) was localized in islet nerves. to learn more about the neuropeptide y-like peptides in islet nerves, we have employed immunohistochemic ... | 1989 | 2673525 |
| oxytocin-like immunoreactive nerves are associated with insulin-containing cells in pancreatic islets of anglerfish (lophius americanus). | recent reports indicate that oxytocin exerts direct effects on the release of insulin and glucagon from the endocrine pancreas of the rat. the purpose of this study was to determine whether oxytocin-like immunoreactivity is present in the anglerfish islet, and if it is associated with subsets of hormone-producing cells. antisera against oxytocin, insulin, glucagon, somatostatin, neuropeptide y, and the 200-kd neurofilament polypeptide were applied to serial 5 micrometers sections of pancreatic i ... | 1987 | 3304646 |
| cold-stable microtubules from antarctic fishes contain unique alpha tubulins. | the cytoplasmic microtubules of antarctic fishes assemble from their tubulin subunits at physiological body temperatures in the range -2 to +2 degrees c. our objective is to determine the structural features that enhance the assembly of antarctic fish tubulins at low temperatures. here we compare the structures of tubulin subunits from three antarctic fishes (notothenia gibberifrons, notothenia coriiceps neglecta, and chaenocephalus aceratus), from three temperate fishes (the dogfish shark muste ... | 1987 | 3597376 |
| the ultrastructure of spores (protozoa: microsporida) from lophius americanus, the angler fish. | spinal and cranial ganglia of american angler fish, lophius americanus, are often infected with microsporidia. this protozoon elicits the formation of large, spore-filled, hypertrophied host cells, cysts. previous reports of microsporidia in european lophiids identify the parasite as spraguea lophii, a genus which has recently been shown to be dimorphic. the spores from l. americanus are monomorphic (2.8 x 1.5 micron) and uninucleate. each spore contains a polar tube that forms six to nine coils ... | 1986 | 3795144 |
| regulation of hormone biosynthesis in cultured islet cells from anglerfish. | the effects of glucose and arginine on islet hormone biosynthesis were investigated using primary cell cultures prepared from islets of the anglerfish (lophius americanus). after dispersion under sterile conditions, islet cells were maintained at 23 degrees c in medium containing rpmi 1640 with hanks' buffer, ph 7.5, modified by the adjustment of glucose (to 0.56 or 5.6 mm) and arginine (to 0.1, 1.15, or 10 mm) with the addition of 10% fetal bovine serum (dialyzed, heat inactivated) and penicill ... | 1985 | 3910637 |
| evidence for glucagon biosynthesis involving a protein intermediate in islets of the anglerfish (lophius americanus). | | 1971 | 4935778 |
| in vitro biosynthesis of fish islet preprosomatostatin: evidence of processing and segregation of a high molecular weight precursor. | evidence is presented for a precursor to somatostatin that is 10-12 times larger than the authentic secreted hormone. mrna from angler fish (lophius americanus) islets of langerhans was translated in the wheat germ cell-free system and the products were identified by immunoprecipitation with specific antibodies to somatostatin followed by sodium dodecyl sulfate gel electrophoresis. one 18,000-dalton polypeptide was specifically immunoprecipitable. competition experiments showed that authentic so ... | 1980 | 6107904 |
| comparison of the nucleic acid sequence of anglerfish and mammalian insulin mrna's from cloned cdna's. | anglerfish (lophius americanus) insulin complementary dna was cloned in bacterial plasmids, and its sequence was determined. fish insulin messenger rna is larger (1.5 times) than the messenger rna encoding mammalian (rat and human) insulin, in part because of a larger c peptide (an additional six amino acids or 18 nucleotides in length) but mainly because of increases in the 5' and 3' untranslated regions. comparison of the fish, rat, and human insulin messenger rna (from the complementary dna) ... | 1980 | 7001633 |
| tubular secretion of creatine, trimethylamine oxide, and other organic bases by the aglomerular kidney of lophius americanus. | creatine and trimethylamine oxide (tmao) are the chief nitrogenous constituents of normal lophius urine, and both of these organic bases characteristically have high urine/plasma concentration ratios. competition studies involving various organic bases indicate that creatine and tmao are excreted independently by separate transport mechanisms. tmao excretion is inhibited competitively by tetraethylammonium ion (tea) and by cyanine dye no. 863-compounds previously shown to be transferred actively ... | 1958 | 13587915 |
| major contributions of comparative endocrinology to the development and exploitation of the incretin concept. | an incretin is a factor released by the gut in response to nutrients that facilitates uptake of glucose by peripheral tissues. the incretin concept predates the discovery of insulin but it is now clear that incretins act by stimulating secretion of this hormone. as glucagon has insulin-releasing activity, it was speculated that intestinal glucagon-like immunoreactivity (enteroglucagon) was involved in the incretin effect but it was an achievement in the field of comparative endocrinology that le ... | 2006 | 16902971 |
| metal concentrations in monkfish, lophius americanus, from the northeastern usa. | concentrations of arsenic (as), selenium (se), lead (pb), zinc (zn), cadmium (cd), and total mercury (hg) were analyzed in the muscle, liver, and gonads of the american monkfish, lophius americanus, collected from franklin swell, massachusetts; mud hole, new jersey; and the fingers, maryland, in the northeastern us, by commercial gillnetters from february to may 2007. results indicated that as and hg concentrations were significantly higher in liver samples collected from the fingers than from m ... | 2011 | 20714929 |
| primary structure of insulin and glucagon from the flounder (platichthys flesus). | insulin and glucagon have been isolated from the brockmann bodies of the flounder, a teleostean fish, and their primary structures established by automated edman degradation. the a-chain of flounder insulin shows strong homology to the a-chains from the coho salmon (oncorhynchus kisutch; 100%) and the anglerfish (lophius americanus; 95%) but homologies in the b-chain region are weaker (salmon 79%, anglerfish 83%). flounder insulin b-chain contains the novel sequence val-val-pro-pro at the nh2 te ... | 2012 | 3556313 |
| primary structure of glucagon from an elasmobranchian fish. torpedo marmorata. | glucagon has been isolated from the pancreas of torpedo marmorata, an elasmobranchian cartilaginous fish, and purified to homogeneity using only reverse-phase high-performance liquid chromatography. amino acid sequence analysis indicates that the molecule differs from mammalian glucagon at position 3 (glutamic acid for glutamine), position 16 (asparagine for serine), and position 20 (lysine for glutamine). extracts of t. marmorata intestine and brain were associated with glucagon-like immunoreac ... | 2014 | 4076759 |