| pollution of the sea and its effects. | marine pollution has been studied under the following groupings of effects; harm to living resources, hazards to human health, reduction of amenities and interference with other users of the sea. this paper is concerned mainly with the first two categories and their interrelation. apart from certain seabirds affected by oil, the major stocks of marine animals show no evidence of reduction by pollution. pollution effects are generally insignificant in relation to other factors governing reproduct ... | 1979 | 40238 |
| assay of staphylococcal delta-haemolysin with fish erythrocytes. | suspensions of erythroctes from certain marine fish (cod, saithe, haddock and mackerel) were 4--16 times more sensitive than human or horse erythrocytes to staphylococcal delta-haemolysin. cod erythrocytes were lysed rapidly by delta-haemolysin and the titres of haemolysin were independent of the temperatures at which the tests were incubated, over the range 0--37 degrees c. fish erythrocytes were not lysed by purified alpha- and beta-haemolysins and no synergistic action was found between beta- ... | 1978 | 355632 |
| microbiological quality of frozen breaded fish and shellfish products. | a survey was made of the microbiological quality of seven frozen, breaded, precooked fish and shellfish products and of frozen, breaded, uncooked shrimp at the retail level. geometric mean aerobic plate counts per gram (and number of units examined) were as follows: fish sticks, 8,300 (1,539); fish cakes, 5,600 (1,378); crab cakes, 4,900 (1,226); scallops, 1,700 (1,392); clams, 450 (1,384); haddock, 15,000 (1,306); fish in fish and chips dinner, 7,200 (1,485); and uncooked shrimp, 220,000 (1,462 ... | 1976 | 779643 |
| isolation of aeromonas sp. atcc 29063, a phenol-producing organism, from fresh haddock. | attempts to isolate phenol-producing organisms from stale haddock fillets failed. several such isolates, however, were readily obtained from fresh haddock and were designated aeromonas sp. phenol was produced from l-tyrosine by these isolates. | 1975 | 1147610 |
| the radio-opacity of fishbones. | plain radiographs are often taken to localize fishbones which lodge in the pharynx. in this study a pig's neck was used to determine the radio-opacity of 14 different species of fishbones. radiographs were taken with bones placed in up to four of the common sites of impaction and assessed on two occasions independently by two previously uninvolved ent consultants. the visibility of the fishbones was scored. inter and intra-observer agreement for species and site was determined. there was excelle ... | 1992 | 1493627 |
| changes in serum and urinary uric acid levels in normal human subjects fed purine-rich foods containing different amounts of adenine and hypoxanthine. | the effect of ingesting some purine-rich foods (beef liver, haddock fillets and soybeans) on uric acid metabolism was investigated in 18 male subjects with no history of gout or kidney disorder. in a crossover design, three isoenergetic and isonitrogenous meals were fed to volunteers during a 3-week period. only the content of uricogenic bases (adenine and hypoxanthine) varied among the test meals. ingestion of all experimental meals caused an increase in serum uric acid levels at 120 minutes an ... | 1992 | 1619189 |
| the radio-opacity of fishbones--species variation. | plain radiographs are often taken to localize fishbones which lodge in the oropharynx and hypopharynx, but which are not seen on clinical examination. fishbones which are thus revealed can then be removed by endoscopy. for a lateral neck radiograph to be useful in excluding a fishbone when mirror examination is difficult or fails to reveal a foreign body, it is important to know which types of fishbone are radio-opaque. we used a pig's neck preparation to simulate a human neck to determine the r ... | 1991 | 1884575 |
| myosin isoforms in red and white muscles of some marine teleost fishes. | the myosin content from red and white muscles of three marine fish species, saithe (pollachius virens. l.), haddock (melanogrammus aeglefinus, l.), both members of the family gadidae, and capeline (mallotus villosus, m.) of the family osmeridae, was analyzed electrophoretically. analysis of the native myosin by electrophoresis under non-dissociating conditions revealed two isoforms in red muscles, and three or four in white muscles. the white muscles of the two closely related species had a simi ... | 1990 | 2150675 |
| the histamine degradative uptake pathway in human vascular endothelial cells and skin fibroblasts is dependent on extracellular na+ and cl-. | we have previously reported that human vascular endothelial cells and skin fibroblasts carry out degradation of [3h]histamine by a mechanism involving two successive enzymatic steps: imidazole ring tele-methylation by the cells' endogenous methyltransferase and subsequent amine oxidation by an exogenous diamine oxidase. both histamine and the exogenous second enzyme in the pathway associate with the cells via separate binding sites or receptors. the enzymatic degradation process results in cellu ... | 1990 | 2201685 |
| morphology of diplomonad flagellates: spironucleus torosa n. sp. from atlantic cod gadus morhua l., and haddock melanogrammus aeglefinus (l.) and hexamita salmonis moore from brook trout salvelinus fontinalis (mitchill). | a diplomonad flagellate, spironucleus torosa n. sp. is described from atlantic cod gadus morhua and haddock melanogrammus aeglefinus. this is believed to be the 1st confirmed report of spironucleus from a marine fish. organisms swimming in the rectal lumen were broadly pyriform to elongate, and measured 10.5-18.6 microns long and 3.2-13.3 microns wide; other elongate organisms were attached to the rectal epithelium, via apical extensions appearing continuous with the microvilli. the posterior en ... | 1990 | 2213651 |
| uricogenic potential of selected cooked foods in rats. | samples of beefsteak, beef liver, haddock, and mushrooms were cooked by two methods: boiling and broiling. growth and purine metabolism in rats fed diets containing 20% protein (n x 6.25) were studied for a period of 26 days. the cooked meats and fish were used as the sole source of dietary protein, while cooked mushrooms provided 5% protein in mushroom-casein diets. body weight, and food and water intakes were recorded weekly while total urine was collected during the last 6 days of the feeding ... | 1990 | 2358620 |
| electrophoretic study of myosin isoforms in white muscles of some teleost fishes. | 1. white skeletal muscle myosin of four marine teleost fish species (cod, blue whiting, norway haddock, and spotted wolf-fish) was analyzed by native, sds-page, and 2-dimensional electrophoresis. 2. four types of native myosin were present in cod, blue whiting and norway haddock. the second fastest migrating form was predominant. 3. myosin from spotted wolf-fish also resolved into four forms. the fastest migrating form was hardly noticeable. the other three were present in apparently similar amo ... | 1990 | 2361358 |
| new aspects of prolactin and immunity: a lymphocyte-derived prolactin-like product and nuclear protein kinase c activation. | in addition to its growth regulating properties, new evidence reviewed here by diane haddock russell demonstrates that prolactin has important immunoregulatory properties. in immune-compromised dwarf mice, prolactin restores immunocompetence. human lymphocytes have prolactin receptors and mitogen-stimulated lymphocytes make and secrete a prolactin-like activity. prolactin can stimulate the activation of nuclear protein kinase c in spleen and liver isolated nuclear preparations. this activation i ... | 1989 | 2688216 |
| characterization of lens crystallins and their mrna from the carp lenses. | crystallins from carp eye lenses have been isolated and characterized by gel permeation chromatography, sds-gel electrophoresis, immunodiffusion and amino acid analysis. gamma-crystallin is the most abundant class of crystallins and constitutes over 55% of the total lens cytoplasmic proteins. it is immunologically distinct from the alpha- and beta-crystallins isolated from the same lens and its antiserum shows a very weak cross-reaction to total pig lens antigens. comparison of the amino acid co ... | 1986 | 3707973 |
| respiration-driven proton translocation by yeast mitochondria with differing efficiencies of oxidative phosphorylation. | measurements were made of the stoicheiometry of proton translocation coupled to respiration in mitochondria from candida utilis where the number of functional energy-conservation sites between intramitochondrial nadh and oxygen was one in a mutant with a novel oxidase (downie & garland, 1972), two in sulphate-deficient cells (haddock & garland, 1971) or three in glycerol-limited cells (light & garland, 1971). the stoicheiometries of protons translocated per atom of oxygen utilized (i.e. -->h(+)/ ... | 1973 | 4357710 |
| amino and carboxy terminal sequence of -crystallin, from haddock lens. | | 1973 | 4685070 |
| nonspecific toxicites in the mouse assay test for botulinum toxin. | in inoculated pack experiments on clostridium botulinum type e, unirradiated and 0.1-mrad irradiated haddock fillets often gave nonspecific toxicities by the mouse assay test for botulinum toxin. samples given 0.2-mrad radiation failed to produce nonspecific reactions. nonspecific deaths sometimes occurred within 24 hr after injection, although deaths between 24 and 48 hr were more common. the symptoms and the pattern of these deaths suggested a septicemia. heart-blood cultured from mice showing ... | 1968 | 4877496 |
| detection and incidence of specific species of spoilage bacteria on fish. ii. relative incidence of pseudomonas putrefaciens and fluorescent pseudomonads on haddock fillets. | pseudomonas putrefaciens has been found to constitute one of the major species of spoilage bacteria on haddock fillets. the initial population of this organism on fillets of high bacterial quality is uniformly below 4% and most frequently no greater than 1%. during refrigerated storage, the organism increases at a more rapid rate than the total psychrophilic population, comprising 50 to 90% of the total population when the total count exceeds 10(6)/g of tissue. fluorescent pseudomonads were show ... | 1968 | 4881956 |
| the effect of gamma rays on haddock and clams inoculated with clostridium botulinum, type e. tid-24885. | | 1965 | 4902856 |
| minimal growth temperature, sodium chloride tolerance, ph sensitivity, and toxin production of marine and terrestrial strains of clostridium botulinum type c. | minimal growth temperatures of four marine and two terrestrial strains of clostridium botulinum type c were determined in a laboratory culture medium, fortified egg meat medium (fem), and in ground haddock. the inoculum equaled 2 x 10(6) viable spores per tube with five-tube replicate sets. the spores were preheated in aqueous suspension at 71 c for 15 min prior to inoculation to reduce toxin carry-over. similar results were obtained in both substrates. both the marine and the terrestrial strain ... | 1971 | 4944801 |
| a survey on the occurrence of vibrio parahaemolyticus on fish and shellfish, marketed in the netherlands. | a survey was carried out on the occurrence of vibrio parahaemolyticus on fish and shellfish, as sold in the netherlands.the optimal mode of detection of this bacterium appeared to be: (i) enrichment of swabs taken from the surface and the gills in freshly prepared meat broth with 5% nacl; (ii) streaking onto teepol bromothymol blue agar (btb) and taurocholate bromothymol blue sucrose agar (tcbs); (iii) confirmation of suspect colonies by testing for mode of growth in butts/slants of a kligler ty ... | 1970 | 5270202 |
| thermal inactivation of type e botulinum toxin. | the theoretical required cooking times for inactivation of type e clostridium botulinum toxin (5,000 ld(50) mouse units per 0.5 ml) in haddock fillets of various sizes were calculated by graphical integration of the toxin inactivation rate and heat penetration data. the results indicated that normal cooking procedures should suffice to inactivate this amount of toxin. this conclusion was substantiated by the following additional experimental observations which revealed that the original experime ... | 1967 | 5339838 |
| detection and incidence of specific species of spoilage bacteria on fish. i. methodology. | the ability of pseudomonas putrefaciens to form h(2)s was found to serve as a singularly useful criterion of identity for this species and was used to directly enumerate the organism from haddock fillets by the use of pour plates of peptone-iron agar. subsurface colonies appear intensely black, whereas surface colonies are black or gray. a highly sensitive soft-agar-gelatin overlay technique has been found useful for directly determining the numbers of weakly and strongly proteolytic organisms f ... | 1968 | 5726148 |
| extracellular nuclease activity of fish spoilage bacteria, fish pathogens, and related species. | the production of extracellular deoxyribonuclease and ribonuclease by 23 marine and 3 dairy strains of pseudomonas putrefaciens, 15 strains of fish-pathogenic fluorescent pseudomonads, 38 strains of fluorescent pseudomonads isolated from haddock, and 34 related organisms was determined by an agar plate method. all strains of p. putrefaciens produced both deoxyribonuclease and ribonuclease. of the other 87 organisms examined, 26.5% produced ribonuclease and 14.5% produced deoxyribonuclease. all o ... | 1969 | 5797936 |
| effect of packaging on the major microbial flora of irradiated haddock. | | 1967 | 6067600 |
| ultrastructural studies on the heart of bony-fish larvae. | the ultrastructure of the heart in 2 and 6 d old larvae of melanogrammus aeglefinus and 1, 7, 14, and 21 d old larvae of poecilia reticulata, is described. additional studies were done on prenatal specimens of p. reticulata. in the atrium of m. aeglefinus the endocardium is separated from the muscle wall by a wide, electron lucent space, which probably contains cardiac jelly (davis 1924). the myocardium is an 1 cell-thick layer in the atrium, whereas it consists of 2 to 4 cells in the ventricle. ... | 1984 | 6468890 |
| recovery of endogenous selenium from fish tissues by open system dry ashing. | quantitative recovery of endogenous selenium from fish tissues following dry ashing techniques has not been confirmed. an open system dry ashing procedure, using oxidative fusion with mg(no3)2 x 6h2o in the presence of mgo, was tested for recovery of selenium from national bureau of standards (nbs) reference materials (tuna and oyster) and selected fish tissues. recovery after dry ashing and hydride analysis was complete from nbs bovine liver, tuna, and oyster tissues, as well as from food and d ... | 1982 | 7130086 |
| histamine degradative uptake by cultured human pulmonary vascular endothelial cells utilizes an inflammatory cell diamine oxidase. | neutrophil-endothelial interactions, altered clearance properties of the lung toward vasoactive mediators, and damaging effects of histamine that target the lung represent prominent elements of the inflammatory response at the systemic level. the pulmonary vasculature is unusual in that, unlike other tissues and vascular beds, it normally does not metabolize circulating histamine in vivo, although histamine-metabolizing enzyme activities have been detected in disrupted lung tissue. we have there ... | 1994 | 7806511 |
| isolation and characterization of tropomyosin from fish muscle. | a comprehensive survey of tropomyosin from various fish myotomal muscles is reported. the fish tropomyosins were blocked at the n-terminus and, as expected, were found to be of similar amino acid composition, alpha-helical content (> 90% at 10 degrees c) and molecular weight to other vertebrate striated muscle forms. the tropomyosins of salmonids and herring muscle were noticeably heterogeneous when assessed by 2d-page. the distribution of isoforms was tissue-specific: slow muscle contained alph ... | 1994 | 8205394 |
| in vitro digestibility of different prey species of minke whales (balaenoptera acutorostrata). | information on diet composition, daily energy expenditure, energy storage and the utilization of energy in the prey are important factors when evaluating the food consumption of minke whales (balaenoptera acutorostrata) during their summer stay in northern waters. the purpose of the present study was in this context to obtain information on the digestible energy (de) of different prey selected by minke whales. an in vitro three-stage digestion technique, simulating the different compartments of ... | 1993 | 8260475 |
| a multi-observer study examining the radiographic visibility of fishbone foreign bodies. | the use of plain radiographs to localize a suspected fishbone foreign body is the subject of controversy. accordingly radiographs of 14 species of fishbone, impacted in a soft tissue phantom, were assessed by a series of observers from the ent department (consultant surgeons, senior registrars and house officers). the agreement was assessed by graphical description of the data and tested by a spearman's rank correlation test. the overall results showed that, for the clinician, radiography is ver ... | 1996 | 8709081 |
| fatty acid composition of commercially manufactured omega-3 enriched pork products, haddock, and mackerel. | this study was conducted to determine the commercial feasibility of feeding a 15% ground flaxseed diet to finishing hogs for up to 42 d before slaughter and to compare the fatty acid composition of the resulting pork products with commercially produced haddock and mackerel. eighty-seven pigs were fed a control diet (predominantly corn, soybean meal-based) and then a similar diet containing 15% flaxseed for the last 28 (fs28) or 42 d (fs42) before slaughter. control pigs were continued on the con ... | 1997 | 9303450 |
| sickling of anoxic red blood cells in fish. | the occurrence of the mutant hemoglobin hb s in human red blood cells results in sickle cell anemia. this disease, including its genetic and molecular bases, has been extensively investigated and is well understood (1,2). the presence of deoxy-induced sickling of animal erythrocytes is largely unknown, however. we examined red blood cells (rbcs) from several fish species in vitro under aerated and anoxic conditions. our polarized light microscopic techniques were aimed at establishing correlatio ... | 1998 | 9739547 |
| electron microscopical identification of the flagellate spironucleus torosa (hexamitidae) from burbot lota lota (gadidae) with comments upon its probable introduction to this freshwater host. | hexamitid flagellates from the rectum of the freshwater gadid burbot, from the river glomma in southeastern norway, were studied by scanning and transmission electron microscopy. the surface morphology of the flagellates was consistent with that of spironucleus torosa, previously only found in the cod, haddock, and saithe, all marine gadids. posteriolateral depressions with central protrusions were present in the flagellates, a feature reported from s. torosa only. further, the microtubular cyto ... | 1998 | 9794636 |
| regiospecificity of dioxygenation of di- to pentachlorobiphenyls and their degradation to chlorobenzoates by the bph-encoded catabolic pathway of burkholderia sp. strain lb400. | burkholderia sp. strain lb400 is one of the most potent aerobic polychlorobiphenyl (pcb)-degrading microorganisms that have been characterized. its pcb-dioxygenating activity originates predominantly or exclusively from the biphenyl dioxygenase encoded by its bph gene cluster. analysis of the dioxygenation products of several di- to pentachlorinated biphenyls formed by this enzyme revealed a complex dependence of the regiospecificity and the yield of dioxygenation on the substitution patterns of ... | 1999 | 10427057 |
| the genera ceratomyxa thélohan, 1892, leptotheca thélohan, 1895 and sphaeromyxa thélohan, 1892 (myxosporea: bivalvulida) in gadid fish of the northeast atlantic. | the gall-bladders of four species of gadid fish from the north sea and norwegian waters were examined for myxosporeans. the host species were cod gadus morhua l. (350 examined), haddock melanogrammus aeglefinus (l.) (592 examined), saithe pollachius virens (l.) (205 examined) and whiting merlangius merlangus (l.) (368 examined). four species of myxosporeans are redescribed from these fish. ceratomyxa arcuata thélohan, 1892 was the most common species and was found in whiting (41.8%) and cod (0.6 ... | 1999 | 10619068 |
| [brazilian nursing magazine--a history of resistance]. | part of the conquest of anedb, since 1920 the reben itself inserting in the professional dispute about the diffusion of the nursing facts. ought to edith magalhães fraenkel and rachel haddock lobo the inspiration and initiative for the creation, since 1929, within the participation in the i quadrienal of icn congress, in montreal where were discussed facilities, difficulties and advance of nursing newspaper. the annaes of nursing, edited by the newspaper "journal do brasil--r.j.", in 1932, was t ... | 1998 | 10776303 |
| isolation and identification of viral haemorrhagic septicaemia (vhs) viruses from cod gadus morhua with the ulcus syndrome and from haddock melanogrammus aeglefinus having skin haemorrhages in the north sea. | during fish disease surveys for marine rhabdoviruses in 1993 and 1995, the cod ulcus syndrome was seen widely in all ages of cod, especially the 2 to 5+ year classes. viral haemorrhagic septicaemia virus (vhsv) was isolated from a small proportion of the lesion-positive fish and these isolates were identified by immunofluorescence or elisa. a serendipitous observation of dermal petechiae on haddock was made. vhsv was isolated from this lesion for the first time indicating a new host species for ... | 2000 | 10950186 |
| expression of pineal ultraviolet- and green-like opsins in the pineal organ and retina of teleosts. | in teleostean bony fishes, studies on the adults of various species have shown that pineal photoreceptors are maximally sensitive to short- and middle-wavelength light, possibly utilising both rod-like and pineal-specific opsins. until recently, however, very little was known about the pineal opsins present in embryonic and larval teleosts and their relationships to opsins expressed by retinal photoreceptors. our immunocytochemical studies have revealed that, in atlantic halibut, herring and cod ... | 2001 | 11511667 |
| methylamine in human urine. | methylamine is the simplest aliphatic amine found in human urine. in the body it is thought to play a significant part in central nervous system disturbances observed during renal and hepatic disease and also has a role in general toxicity caused by oxidative stress. the present study provides data on the daily urinary excretion of methylamine in a population of unrelated healthy volunteers and investigates a variety of food substances as potential dietary sources. | 2001 | 11580915 |
| advances in the stable isotope-mass spectrometric measurement of dna synthesis and cell proliferation. | methods for measuring rates of dna synthesis, and thus cell proliferation, in humans had not been available until recently. we (d. c. macallan, c. a. fullerton, r. a. neese, k. haddock, s. s. park, and m. k. hellerstein, 1998, proc. natl. acad. sci. usa 95, 708-713) recently developed a stable isotope-mass spectrometric technique for measuring dna synthesis by labeling the deoxyribose (dr) moiety of purine deoxyribonucleotides through the de novo nucleotide synthesis pathway. the original analyt ... | 2001 | 11700973 |
| distribution of viral haemorrhagic septicaemia virus in wild fish species of the north sea, north east atlantic ocean and irish sea. | a surveillance programme was initiated on the occurrence and distribution of viral haemorrhagic septicaemia virus (vhsv) in wild marine fish. six research cruises were undertaken in an 18 mo period during 1997 and 1998, covering the north sea, the atlantic waters off the north and west coasts of scotland and the irish sea. a total of 19,293 fish were sampled from 23 different species including cod, haddock, norway pout, herring and sprat. individual fish lengths were recorded and the fish were c ... | 2001 | 11775798 |
| temperature and ca(2+)-dependence of the sarcoplasmic reticulum ca(2+)-atpase in haddock, salmon, rainbow trout and zebra cichlid. | temperature dependence of ca(2+)-atpase from the sarcoplasmic reticulum (sr) in rabbit muscle has been widely studied, and it is generally accepted that a break point in arrhenius plot exist at approximately 20 degrees c. whether the break point arises as a result of temperature dependent changes in the enzyme or its membrane lipid environment is still a matter of discussion. in this study we compared the temperature dependence and ca(2+)-dependence of sr ca(2+)-atpase in haddock (melanogrammus ... | 2002 | 12223210 |
| haddock: a protein-protein docking approach based on biochemical or biophysical information. | the structure determination of protein-protein complexes is a rather tedious and lengthy process, by both nmr and x-ray crystallography. several methods based on docking to study protein complexes have also been well developed over the past few years. most of these approaches are not driven by experimental data but are based on a combination of energetics and shape complementarity. here, we present an approach called haddock (high ambiguity driven protein-protein docking) that makes use of bioch ... | 2003 | 12580598 |
| an improved selective isolation medium for the recovery of listeria monocytogenes from smoked fish. | the aim of this study was to improve the selective isolation of listeria monocytogenes from smoked haddock fillets. | 2003 | 12641717 |
| aqueous extracts from some muscles inhibit hemoglobin-mediated oxidation of cod muscle membrane lipids. | it was evaluated whether trout hemoglobin (hb)-mediated oxidation of minced washed cod muscle lipids could be prevented by an aqueous isolate from cod and some other muscle sources. lipid hydroperoxides and painty odor developed approximately 4 days faster in washed than unwashed cod mince. when adding back an aqueous fraction (press juice) isolated from unwashed mince to washed mince at 2-6-fold dilutions, development of hydroperoxides and painty odor was either delayed or completely prevented. ... | 2003 | 12720401 |
| four years of monitoring for viral haemorrhagic septicaemia virus in marine waters around the united kingdom. | between 1995 and 1998, marine fish from around the coast of the uk were collected and samples analysed for viral haemorrhagic septicaemia virus (vhsv) using cell culture isolation methods. in 1997 and 1998 the samples were also analysed for vhsv by reverse transcription pcr (rt-pcr). a total of 1867 fish of 11 species were tested, but vhsv was isolated on only 1 occasion, from herring clupea harengus, in 1996. however, despite vhsv not being isolated in 1997 and 1998, in both years samples of he ... | 2003 | 12803381 |
| changes in the microflora of haddock fillets and shucked soft-shelled clams after irradiation with co-60 gamma rays and storage at 0 c and 6 c. | frequency distribution patterns of aerobic-facultative microflora, obtained by random selection of macrocolonies from samples of haddock fillets and shucked soft-shelled clams before and after treatment with doses of from 50,000 to 800,000 rad of co(60) gamma rays, are presented, with analyses and interpretation. the data showed that a decided change occurred in the constitution of the microbial populations of both products: from a mixed gram-negative-gram-positive flora to a predominantly gram- ... | 1963 | 13933509 |
| radiation-resistant, pigmented coccus isolated from haddock tissue. | davis, norman s. (massachusetts institute of technology, cambridge), gerald j. silverman, and edmund b. masurovsky. radiation-resistant, pigmented coccus isolated from haddock tissue. j. bacteriol. 86:294-298. 1963.-an orange-brown, catalase-positive coccus was isolated from irradiated haddock. the new coccus was found to consist of rough and smooth strains as well as strains possessing appreciably less pigment. the smooth strain (248) was more radioresistant to gamma radiation than the rough st ... | 1963 | 14058955 |
| insight into molecular interactions between two pb1 domains. | specific protein-protein interactions play crucial roles in the regulation of any biological process. recently, a new protein-protein interaction domain termed pb1 (phox and bem1) was identified, which is conserved throughout evolution and present in diverse proteins functioning in signal transduction, cell polarity and survival. here, we investigated the structure and molecular interactions of the pb1 heterodimer complex composed of the pb1 domains of the yeast proteins bem1 and cdc24. a struct ... | 2004 | 15037079 |
| structural model of the ubch5b/cnot4 complex revealed by combining nmr, mutagenesis, and docking approaches. | the protein cnot4 possesses an n-terminal ring finger domain that acts as an e3 ubiquitin ligase and specifically interacts with ubch5b, a ubiquitin-conjugating enzyme. the structure of the cnot4 ring domain has been solved and the amino acids important for the binding to ubch5b have been mapped. here, the residues of ubch5b important for the binding to cnot4 ring domain were identified by nmr chemical shift perturbation experiments, and these data were used to generate structural models of the ... | 2004 | 15062086 |
| a docking approach to the study of copper trafficking proteins; interaction between metallochaperones and soluble domains of copper atpases. | a structural model of the transient complex between the yeast copper chaperone atx1 and the first soluble domain of the copper transporting atpase ccc2 was obtained with haddock, combining nmr chemical shift mapping information with in silico docking. these two proteins are involved in copper trafficking in yeast cells. calculations were performed starting with the copper ion either bound to atx1 or to ccc2 and using the experimental structures of the copper-loaded and apo forms of each protein. ... | 2004 | 15062089 |
| nmr and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist: structural implications for the mhc ii (i-au)-peptide complex from docking calculations. | experimental autoimmune encephalomyelitis can be induced in susceptible animals by immunodominant determinants of myelin basic protein (mbp). to characterize the molecular features of antigenic sites important for designing experimental autoimmune encephalomyelitis suppressing molecules, we report structural studies, based on nmr experimental data in conjunction with molecular dynamic simulations, of the potent linear dodecapeptide epitope of guinea pig mbp, gln74-lys75-ser76-gln77-arg78-ser79-g ... | 2004 | 15291817 |
| the orientations of cytochrome c in the highly dynamic complex with cytochrome b5 visualized by nmr and docking using haddock. | the interaction of bovine microsomal ferricytochrome b5 with yeast iso-1-ferri and ferrocytochrome c has been investigated using heteronuclear nmr techniques. chemical-shift perturbations for 1h and 15n nuclei of both cytochromes, arising from the interactions with the unlabeled partner proteins, were used for mapping the interacting surfaces on both proteins. the similarity of the binding shifts observed for oxidized and reduced cytochrome c indicates that the complex formation is not influence ... | 2005 | 15689516 |
| morphometric discrimination of two allozymically diagnosed sibling species of the echinorhynchus gadi zoega in muller complex (acanthocephala) in the north sea. | allozyme electrophoresis was used to detect biological species of the e. gadi complex from gadids from the northern north sea. a fixed difference at one of nine enzyme loci surveyed confirmed the existence of two reproductively isolated, sympatric species. mixed infections of two e. gadi spp. (termed a and b) were observed in gadus morhua and pollachius virens. e. gadi sp. b was also found in melanogrammus aeglefinus and merlangius merlangus. the presence of gravid females of e. gadi spp. a and ... | 2005 | 15841350 |
| life cycle strategy of hysterothylacium aduncum to become the most abundant anisakid fish nematode in the north sea. | the present study demonstrates the influence of physical systems (mixed and stratified waters) on the occurrence and distribution of the anisakid nematode hysterothylacium aduncum in commercially important gadiform fish species from the central and northern north sea. stratified waters are essential in structuring marine food webs and are therefore also important for the transfer of fish parasites to their host species. during two research cruises in 2001, 308 haddocks (melanogrammus aeglefinus) ... | 2005 | 15986247 |
| structural basis of arnt pas-b dimerization: use of a common beta-sheet interface for hetero- and homodimerization. | the aryl hydrocarbon receptor nuclear translocator (arnt) is a promiscuous bhlh-pas (per-arnt-sim) protein that forms heterodimeric transcriptional regulator complexes with several other bhlh-pas subunits to control a variety of biological pathways, some of which are centrally involved in disease initiation and/or progression. one of these is the hypoxia response pathway, which allows eukaryotic cells to respond to low oxygen tension via the formation of a heterodimeric complex between arnt and ... | 2005 | 16181639 |
| structural basis for sumo-e2 interaction revealed by a complex model using docking approach in combination with nmr data. | the interaction between small ubiquitin-related modifier sumo and its conjugating-enzyme ubc9 (e2) is an essential step in sumo conjugation cascade. however, an experimental structure of such a transient complex is still unavailable. here, a structural model of sumo-3-ubc9 complex was obtained with haddock, combining nmr chemical shift mapping information. docking calculations were performed using sumo-3 and ubc9 structures as input. the resulting complex reveals that the complementary surface e ... | 2005 | 16224784 |
| partial purification and some properties of tyrosine phenol-lyase from aeromonas phenologenes atcc 29063. | tyrosine phenol-lyase was purified 32-fold from aeromonas phenologenes atcc 29063, the organism that produces phenol in refrigerated haddock. the purification procedure included ammonium sulfate fractionation, protamine sulfate treatment, and column chromatography with sephadex g-200, diethyl-aminoethyl-cellulose, and hydroxyapatite. the enzyme was found to be thermally inactivated at temperatures above 40 degrees c. the optimum ph of the enzyme was found to be ph 8.5. the michaelis constants fo ... | 1977 | 16345185 |
| pseudomonads and achromobacters in the spoilage of irradiated haddock of different preirradiation quality. | the effect of initial quality of fish on postirradiation (100 krad) changes in the bacterial flora of haddock fillets during aerobic storage at 3 c has been investigated, with emphasis on the pseudomonas and achromobacter groups. the quality was related to the length of time the eviscerated fish had been stored in ice prior to filleting. increased numbers of organisms, in particular pseudomonas putrefaciens, were found initially on fillets cut from older fish. pseudomonads were reduced by 2 to 3 ... | 1970 | 16349884 |
| timely opportunities for families and their healthcare providers: a commentary on pyle et al. (2005). | this commentary on s. a. pyle, c. k. haddock, n. hymowitz, j. schwab, and s. meshberg (2005) addresses the complex nature of establishing family rules about exposure to tobacco smoke (ets). particular attention is paid to how family rules are established and the challenges in enforcing rules among adult family members. findings are also discussed in terms of the limits of family rule making and the need for negotiation and conflict resolution. the compounding influence of socioeconomic status an ... | 2005 | 16479273 |
| solution structure of the ubiquitin-associated domain of human bmsc-ubp and its complex with ubiquitin. | ubiquitin is an important cellular signal that targets proteins for degradation or regulates their functions. the previously identified bmsc-ubp protein derived from bone marrow stromal cells contains a ubiquitin-associated (uba) domain at the c terminus that has been implicated in linking cellular processes and the ubiquitin system. here, we report the solution nmr structure of the uba domain of human bmsc-ubp protein and its complex with ubiquitin. the structure determination was facilitated b ... | 2006 | 16731964 |
| astaxanthin binding to solubilized muscle proteins of atlantic salmon (salmo salar l.), haddock (melanogrammus aeglefinus l.) and atlantic halibut (hippoglossus hippoglossus l.). | a study was conducted to compare astaxanthin binding ability of solubilized muscle proteins of atlantic salmon (salmo salar l.), haddock (melanogrammus aeglefinus l.) and atlantic halibut (hippoglossus hippoglossus l.). muscle proteins of juvenile atlantic salmon, haddock and halibut were solubilized by sequential extraction of muscle tissue using low ionic strength solutions. electrophoretic protein profiles of the six solubilized fractions from these species were similar. each solubilized frac ... | 2006 | 16781177 |
| variations in the unstructured c-terminal tail of interferons contribute to differential receptor binding and biological activity. | type i interferons (ifns) elicit antiviral, antiproliferative and immunomodulatory properties in cells. all of them bind to the same receptor proteins, ifnar1 and ifnar2, with different affinities. while the 13 known ifnalphas are highly conserved, the c-terminal unstructured tail was found to have large variation in its net charge, from neutral to +4. this led us to speculate that the tail may have a role in modulation of the ifn biological activity, through fine-tuning the binding to ifnar2. t ... | 2006 | 16815442 |
| information-driven protein-dna docking using haddock: it is a matter of flexibility. | intrinsic flexibility of dna has hampered the development of efficient protein-dna docking methods. in this study we extend haddock (high ambiguity driven docking) [c. dominguez, r. boelens and a. m. j. j. bonvin (2003) j. am. chem. soc. 125, 1731-1737] to explicitly deal with dna flexibility. haddock uses non-structural experimental data to drive the docking during a rigid-body energy minimization, and semi-flexible and water refinement stages. the latter allow for flexibility of all dna nucleo ... | 2006 | 16820531 |
| cloning and expression analysis of two pro-inflammatory cytokines, il-1 beta and il-8, in haddock (melanogrammus aeglefinus). | this paper reports the cloning and sequencing of two pro-inflammatory cytokines, interleukin (il)-1beta and il-8, in haddock (melanogrammus aeglefinus) by homology cloning. the complete transcript of the haddock il-1beta was sequenced and contained 1043 bp, including a 762 bp open reading frame. the 3' end of the gene includes a polyadenylation signal 13 bp upstream of the poly(a) tail, along with 10 instability motifs. the predicted protein of 253aa revealed the presence of the il-1 family sign ... | 2007 | 16831460 |
| influence of storage temperature on microbial spoilage characteristics of haddock fillets (melanogrammus aeglefinus) evaluated by multivariate quality prediction. | the proliferation of specific spoilage organisms (sso) and quality changes were evaluated in haddock fillets stored in styrofoam boxes at 0, 7 and 15 degrees c and under temperature fluctuations. a rapid electronic nose technique was used to monitor different classes of compounds, representing microbial metabolites that were characteristic for the onset of spoilage odors. photobacterium phosphoreum predominated among the spoilage bacteria and high levels of tvb-n were observed at sensory rejecti ... | 2006 | 16889858 |
| a cell line (hew) from embryos of haddock (melanogrammus aeglefinius) and its capacity to tolerate environmental extremes. | cell lines can be useful experimental tools for studying marine fish, which are often difficult to routinely obtain and maintain in the laboratory. as few cell lines are available from coldwater marine fish, cultures were initiated from late gastrula embryos of haddock (melanogrammus aeglefinus) in leibovitz's l-15 with fetal bovine serum (fbs). from one culture, a cell line (hew) emerged that has been grown for close to 100 population doublings, was heteroploid, and expressed telomerase activit ... | 2006 | 16909215 |
| atypical aeromonas salmonicida infection in naturally- and laboratory-challenged farmed haddock, melanogrammus aeglefinus (l.). | | 2007 | 17501741 |
| density dependence and density independence during the early life stages of four marine fish stocks. | recruitment variability caused by density-dependent and density-independent processes is an important area within the study of fish dynamics. these processes can exhibit nonlinearities and nonadditive properties that may have profound dynamic effects. we investigate the importance of population density (i.e., density dependence) and environmental forcing (i.e., density independence) on the age-0 and age-1 abundance of capelin (mallotus villosus), northeast arctic cod (gadus morhua), northeast ar ... | 2007 | 17503591 |
| vaccination experiments in the gadoid haddock, melanogrammus aeglefinus l., against the bacterial pathogen vibrio anguillarum. | vibrio anguillarum is one of the primary pathogens responsible for high levels of fish mortality in the aquaculture industry, and among gadoids o2a and b are the most common pathogenic serotypes. in this paper a variety of studies were performed to assess the optimal route by which to challenge haddock against this pathogen, and an optimal regime to vaccinate haddock. the most efficient method to challenge haddock with v. anguillarum in this study was immersion in a bath containing 10(7)cfu/ml, ... | 2007 | 17559945 |
| nmr-based modeling and binding studies of a ternary complex between chicken liver bile acid binding protein and bile acids. | chicken liver bile acid binding protein (cl-babp) is involved in bile acid transport in the liver cytosol. a detailed study of the mechanism of binding and selectivity of bile acids binding proteins towards the physiological pool of bile salts is a key issue for the complete understanding of the role of these proteins and their involvement in cholesterol homeostasis. in the present study, we modeled the ternary complex of cl-babp with two molecules of bile salts using the data driven docking pro ... | 2007 | 17607743 |
| structural insights into the interaction of insulin-like growth factor 2 with igf2r domain 11. | the insulin-like growth factor ii/mannose-6-phosphate receptor (igf2r) mediates trafficking of mannose-6-phosphate (m6p)-containing proteins and the mitogenic hormone igf2. igf2r also plays an important role as a tumor suppressor, as mutation is frequently associated with human carcinogenesis. igf2 binds to domain 11, one of 15 extracellular domains on igf2r. the crystal structure of domain 11 and the solution structure of igf2 have been reported, but, to date, there has been limited success whe ... | 2007 | 17850746 |
| dimethylamine and diet. | forty-six different foods eaten by six healthy male volunteers were investigated as potential sources of the aliphatic secondary amine, dimethylamine. none that were representatives from the fruit and vegetable, meat, dairy and grain produce categories afforded any measurable elevation in urinary dimethylamine output following ingestion. all of the statistically significant increases occurred after consumption of fish and seafoods. however, within this category a wide variation was observed. the ... | 2008 | 18282650 |
| comparison of antimicrobial activity in the epidermal mucus extracts of fish. | the mucus layer on the surface of fish consists of several antimicrobial agents that provide a first line of defense against invading pathogens. to date, little is known about the antimicrobial properties of the mucus of arctic char (salvelinus alpinus), brook trout (s. fontinalis), koi carp (cyprinus carpio sub sp. koi), striped bass (morone saxatilis), haddock (melanogrammus aeglefinus) and hagfish (myxine glutinosa). the epidermal mucus samples from these fish were extracted with acidic, orga ... | 2008 | 18342561 |
| vitellogenin, a biomarker for environmental estrogenic pollution, of reeves' pond turtles: analysis of similarity for its amino acid sequence and cognate mrna expression after exposure to estrogen. | vitellogenin (vtg), a biomarker for environmental estrogenic pollution, can be detected in the bloodstream of oviparous animals before morphological and functional abnormalities appear due to exposure to environmental estrogens. reports observing vtg in turtles have been limited. we therefore cloned and sequenced a partial cdna of vtg in reeves' pond turtle, chinemys reevesii. the cloned cdna fragment possessed the start codon and 2,229 bp, encoding 743 amino acid residues. a sequence of deduced ... | 2008 | 18388421 |
| solution structure of the calponin homology (ch) domain from the smoothelin-like 1 protein: a unique apocalmodulin-binding mode and the possible role of the c-terminal type-2 ch-domain in smooth muscle relaxation. | the smtnl1 protein contains a single type-2 calponin homology (ch) domain at its c terminus that shares sequence identity with the smoothelin family of smooth muscle-specific proteins. in contrast to the smoothelins, smtnl1 does not associate with f-actin in vitro, and its specific role in smooth muscle remains unclear. in addition, the biological function of the c-terminal ch-domains found in the smoothelin proteins is also poorly understood. in this work, we have therefore determined the solut ... | 2008 | 18477568 |
| solution structure of the pseudomonas putida protein ppputa45 and its dna complex. | proline utilization a (puta) is a membrane-associated multifunctional enzyme that catalyzes the oxidation of proline to glutamate in a two-step process. in certain, gram-negative bacteria such as pseudomonas putida, puta also acts as an auto repressor in the cytoplasm, when an insufficient concentration of proline is available. here, the n-terminal residues 1-45 of puta from p. putida (ppputa45) are shown to be responsible for dna binding and dimerization. the solution structure of ppputa45 was ... | 2009 | 18767154 |
| molecular basis of the interaction between complement receptor type 2 (cr2/cd21) and epstein-barr virus glycoprotein gp350. | the binding of the epstein-barr virus glycoprotein gp350 by complement receptor type 2 (cr2) is critical for viral attachment to b lymphocytes. we set out to test hypotheses regarding the molecular nature of this interaction by developing an enzyme-linked immunosorbent assay (elisa) for the efficient analysis of the gp350-cr2 interaction by utilizing wild-type and mutant forms of recombinant gp350 and also of the cr2 n-terminal domains scr1 and scr2 (designated cr2 scr1-2). to delineate the cr2- ... | 2008 | 18786993 |
| identification and structural mechanism for a novel interaction between a ubiquitin ligase wwp1 and nogo-a, a key inhibitor for central nervous system regeneration. | nogo-a has been extensively demonstrated to play key roles in inhibiting central nervous system regeneration, regulating endoplasmic reticulum formation, and maintaining the integrity of the neuromuscular junction. in this study, an e3 ubiquitin ligase wwp1 was first identified to be a novel interacting partner for nogo-a both in vitro and in vivo. by using cd, itc, and nmr, we have further conducted extensive studies on all four wwp1 ww domains and their interactions with a nogo-a peptide carry ... | 2008 | 19035836 |
| nmr structural analysis of dna recognition by a novel myb1 dna-binding domain in the protozoan parasite trichomonas vaginalis. | the transcription regulator, tvmyb1, is the first myb family protein identified in trichomonas vaginalis. using an electrophoretic mobility shift assay, we defined the amino-acid sequence from lys(35) to ser(141) (tvmyb1(35-141)) as the minimal dna-binding domain, encompassing two myb-like dna-binding motifs (designated as r2 and r3 motifs) and an extension of 10 residues at the c-terminus. nmr solution structures of tvmyb1(35-141) show that both the r2 and r3 motifs adopt helix-turn-helix confo ... | 2009 | 19246540 |
| identification of interleukin-22 in gadoids and examination of its expression level in vaccinated fish. | this paper reports the cloning and sequencing of interleukin (il)-22 in two gadoid fish, cod (gadus morhua) and haddock (melanogrammus aeglefinus). the complete transcript of this gene was 1002 and 1154 bp respectively, of which 492 bp was the open reading frame (orf) in both genes. high amino acid identity (88.3%) was found between these genes but was less than 50% aa identity to other known genes. the gene organisation of haddock il-22 consisted of five exons and four introns, as with other il ... | 2009 | 19403174 |
| nonmyristoylated matrix protein from the mason-pfizer monkey virus forms oligomers. | we studied the oligomeric properties of betaretroviral nonmyristoylated matrix protein (ma) and its r55f mutant from the mason-pfizer monkey virus in solution by means of chemical crosslinking and nmr spectroscopy. by analyzing crosslinked products and using concentration-dependent nmr chemical shift mapping, we have proven that the wild-type (wt) ma forms oligomers in solution. conversely, no oligomerization was observed for the r55f mutant. structural comparison of mas explained their differen ... | 2009 | 19481092 |
| purified glycosaminoglycans from cooked haddock may enhance fe uptake via endocytosis in a caco-2 cell culture model. | this study aims to understand the enhancing effect of glycosaminoglycans (gags), such as chondroitin/dermatan structures, on fe uptake to caco-2 cells. high-sulfated gags were selectively purified from cooked haddock. an in vitro digestion/caco-2 cell culture model was used to evaluate fe uptake (cell ferritin formation) from a fe(+3)-containing solution, and fe(+3)/ascorbic acid (aa) and fe(+3)/gags mixtures. mitochondria (mtt test) and endosomal/lysosomal activities (neutral red uptake, nr), i ... | 2009 | 19723201 |
| the structure of the ubch8-ubiquitin complex shows a unique ubiquitin interaction site. | ubiquitin-mediated proteolysis utilizes a series of three key enzymes (e1, e2, and e3) to transfer and then covalently modify a substrate with ubiquitin. e2 conjugating enzymes are central proteins in this pathway responsible for the acceptance of a ubiquitin from the e1 enzyme and association with an e3 protein. all e2 enzymes covalently bind ubiquitin through a thiolester linkage between a conserved active-site cysteine on e2 and the c-terminal glycine on ubiquitin. it is not known whether e2 ... | 2009 | 19928833 |
| ecological meltdown in the firth of clyde, scotland: two centuries of change in a coastal marine ecosystem. | the firth of clyde is a large inlet of the sea that extends over 100 km into scotland's west coast. | 2010 | 20686614 |
| strengths and weaknesses of data-driven docking in critical assessment of prediction of interactions. | the recent capri rounds have introduced new docking challenges in the form of protein-rna complexes, multiple alternative interfaces, and an unprecedented number of targets for which homology modeling was required. we present here the performance of haddock and its web server in the capri experiment and discuss the strengths and weaknesses of data-driven docking. haddock was successful for 6 out of 9 complexes (6 out of 11 targets) and accurately predicted the individual interfaces for two more ... | 2010 | 20718048 |
| description of five new loma (microsporidia) species in pacific fishes with redesignation of the type species loma morhua morrison & sprague, 1981, based on morphological and molecular species-boundaries tests. | five new species of loma were described from five pacific fishes using light-microscopic and ultrastructural features along with phylogenetic analysis of the gene sequences of ribosomal rna (rrna) and elongation factor 1-alpha. morphological data revealed both qualitative and quantitative differences in developmental stages and timing, vesicles, xenoma features, and spore sizes with statistical support that differentiated loma pacificodae n. sp. in pacific cod, loma wallae n. sp. in walleye poll ... | 2010 | 21040100 |
| structure of the mycobacterium tuberculosis ompatb protein: a model of an oligomeric channel in the mycobacterial cell wall. | the pore-forming outer membrane protein ompatb from mycobacterium tuberculosis is a virulence factor required for acid resistance in host phagosomes. in this study, we determined the 3d structure of ompatb by nmr in solution. we found that ompatb is composed of two independent domains separated by a proline-rich hinge region. as expected, the high-resolution structure of the c-terminal domain (ompatb(198-326)) revealed a module structurally related to other ompa-like proteins from gram-negative ... | 2011 | 21117233 |
| binding between a distal c-terminus fragment of cannabinoid receptor 1 and arrestin-2. | internalization of g-protein-coupled receptors is mediated by phosphorylation of the c-terminus, followed by binding with the cytosolic protein arrestin. to explore structural factors that may play a role in internalization of cannabinoid receptor 1 (cb1), we utilize a phosphorylated peptide derived from the distal c-terminus of cb1 (cb1(5p)(454-473)). complexes formed between the peptide and human arrestin-2 (wt-arr2(1-418)) were compared to those formed with a truncated arrestin-2 mutant (tr-a ... | 2011 | 21306178 |
| protein-protein haddocking using exclusively pseudocontact shifts. | in order to enhance the structure determination process of macromolecular assemblies by nmr, we have implemented long-range pseudocontact shift (pcs) restraints into the data-driven protein docking package haddock. we demonstrate the efficiency of the method on a synthetic, yet realistic case based on the lanthanide-labeled n-terminal ε domain of the e. coli dna polymerase iii (ε186) in complex with the hot domain. docking from the bound form of the two partners is swiftly executed (interface rm ... | 2011 | 21626213 |
| molecular basis of the recognition of the ap65-1 gene transcription promoter elements by a myb protein from the protozoan parasite trichomonas vaginalis. | iron-inducible transcription of the ap65-1 gene in trichomonas vaginalis involves at least three myb-like transcriptional factors (tvmyb1, tvmyb2 and tvmyb3) that differentially bind to two closely spaced promoter sites, mre-1/mre-2r and mre-2f. here, we defined a fragment of tvmyb2 comprising residues 40-156 (tvmyb2(40-156)) as the minimum structural unit that retains near full binding affinity with the promoter dnas. like c-myb in vertebrates, the dna-free tvmyb2(40-156) has a flexible and ope ... | 2011 | 21771861 |
| role of egg predation by haddock in the decline of an atlantic herring population. | theoretical studies suggest that the abrupt and substantial changes in the productivity of some fisheries species may be explained by predation-driven alternate stable states in their population levels. with this hypothesis, an increase in fishing or a natural perturbation can drive a population from an upper to a lower stable-equilibrium population level. after fishing is reduced or the perturbation ended, this low population level can persist due to the regulatory effect of the predator. altho ... | 2011 | 21825166 |
| evaluation and comparison of the species-specificity of 3 antiparvalbumin igg antibodies. | parvalbumin is a pan-allergen in fish and frogs that triggers ige-mediated reactions in fish-allergic individuals. previous studies demonstrated that antibodies raised against fish and frog parvalbumins displayed varying specificity for different fish species, and thus, the applicability of these antibodies for potential use in immunoassays to detect fish residues were limited. we aimed to determine the specificity of 3 igg antibodies for various fish species. indirect enzyme-linked immunosorben ... | 2011 | 21999209 |
| defining the limits of homology modelling in information-driven protein docking. | information-driven docking is currently one of the most successful approaches to obtain structural models of protein interactions as demonstrated in the latest round of capri. while various experimental and computational techniques can be used to retrieve information about the binding mode, the availability of three-dimensional structures of the interacting partners remains a limiting factor. fortunately, the wealth of structural information gathered by large-scale initiatives allows for homolog ... | 2013 | 23913867 |
| evolutionarily conserved binding of translationally controlled tumor protein to eukaryotic elongation factor 1b. | translationally controlled tumor protein (tctp) is an abundant protein that is highly conserved in eukaryotes. however, its primary function is still not clear. human tctp interacts with the metazoan-specific eukaryotic elongation factor 1bδ (eef1bδ) and inhibits its guanine nucleotide exchange factor (gef) activity, but the structural mechanism remains unknown. the interaction between tctp and eef1bδ was investigated by nmr titration, structure determination, paramagnetic relaxation enhancement ... | 2015 | 25635048 |
| high sensitivity crosslink detection coupled with integrative structure modeling in the mass spec studio. | the mass spec studio package was designed to support the extraction of hydrogen-deuterium exchange and covalent labeling data for a range of mass spectrometry (ms)-based workflows, to integrate with restraint-driven protein modeling activities. in this report, we present an extension of the underlying studio framework and provide a plug-in for crosslink (xl) detection. to accommodate flexibility in xl methods and applications, while maintaining efficient data processing, the plug-in employs a pe ... | 2016 | 27412762 |
| new insight into the catalytic mechanism of bacterial mray from enzyme kinetics and docking studies. | phospho-murnac-pentapeptide translocase (mray) catalyzes the synthesis of lipid i, a bacterial peptidoglycan precursor. as such, mray is essential for bacterial survival and therefore is an ideal target for developing novel antibiotics. however, the understanding of its catalytic mechanism, despite the recently determined crystal structure, remains limited. in the present study, the kinetic properties of bacillus subtilis mray (bsmray) were investigated by fluorescence enhancement using dansylat ... | 2016 | 27226570 |
| solution structure and dna binding of the catalytic domain of the large serine resolvase tnpx. | the transfer of antibiotic resistance between bacteria is mediated by mobile genetic elements such as plasmids and transposons. tnpx is a member of the large serine recombinase subgroup of site-specific recombinases and is responsible for the excision and insertion of mobile genetic elements that encode chloramphenicol resistance in the pathogens clostridium perfringens and clostridium difficile. tnpx consists of three structural domains: domain i contains the catalytic site, whereas domains ii ... | 2015 | 25720550 |
| solution structure and tandem dna recognition of the c-terminal effector domain of pmra from klebsiella pneumoniae. | klebsiella pneumoniae pmra is a polymyxin-resistance-associated response regulator. the c-terminal effector/dna-binding domain of pmra (pmrac) recognizes tandem imperfect repeat sequences on the promoters of genes to induce antimicrobial peptide resistance after phosphorylation and dimerization of its n-terminal receiver domain (pmran). however, structural information concerning how phosphorylation of the response regulator enhances dna recognition remains elusive. to gain insights, we determine ... | 2014 | 24371275 |