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selected mitochondrial genes as species markers of the arctic contracaecum osculatum complex.this study, aimed at testing the hypothesis that some mitochondrial genes can serve as species-specific markers, involved a comparison of the sequence variance of selected mitochondrial dna genes of the arctic contracaecum osculatum species (c. osculatum a, c. osculatum b and c. osculatum c). we compared differences between five complete (nd2, cytb, nd3, nd4l and nd6) and three partial (co1, co3 and nd5) protein-coding genes. the total length of the sequence of each of the 13 specimens was 4830  ...201121749741
mitochondrial dna of anisakis simplex s.s. as a potential tool for differentiating populations.anisakis simplex s.s. specimens from gadus morhua, gadus ogac, reinhardtius hippoglossoides, clupea harengus, micromesistius poutassou, pleurogrammus monopterygius, and oncorhynchus nerka, caught in the north atlantic and pacific, were examined. identity of the parasite species was confirmed using a molecular key. all specimens were used for amplifying a part of the non-coding sequence of the at-rich region, flanked by the nad6-nad4l-trnw-trne-rrns-trns2, and trnn-trny-nad1 genes. this part of t ...200919530887
gastric proteases of the greenland cod gadus ogac. ii. structural properties.three gastric proteases were isolated from the stomach mucosa of the greenland cod (gadus ogac). the cod proteases were all less stable to heating and protease 1 retained less activity at 5 degrees c when the ph was greater than 5 in comparison with porcine pepsin. the activities of cod proteases 1 and 2, with hemoglobin as the substrate, were doubled in the presence of 25 mm nacl, while cod protease 3 and porcine pepsin were not stimulated by the salt. the cod proteases did not cross-react with ...19863521676
gastric proteases of the greenland cod gadus ogac. i. isolation and kinetic properties.the zymogens of three gastric proteases of the greenland cod (gadus ogac) were isolated by exclusion chromatography and chromatofocusing. the cod zymogens were activated more rapidly at lower temperatures than porcine pepsinogen and, after activation, were further purified by exclusion chromatography. the cod proteases had more alkaline ph optima and were active over a wider range of ph than porcine pepsin. the specific activity of porcine pepsin on protein substrates was greater than that of th ...19863087385
rapid evolution of the intergenic t-p spacer in the mtdna of arctic cod arctogadus glacialis.a noncoding intergenic spacer has previously been reported in mtdna of gadiformes. here we present sequence information from two other cod species and variation within three species to clarify the evolution of this region. a general feature of the t-p spacer is high variation and folding into two or three hairpins. the variation among species both in structure of the region and sequence variation reflects the phylogenetic relationship of the species. a unique pattern is found within arctic cod, ...200818214612
fish host-cestode parasite stable isotope enrichment patterns in marine, estuarine and freshwater fishes from northern canada.cestode parasites from freshwater (threespine stickleback, gasterosteus aculeatus), estuarine (brook charr, salvelinus fontinalis) and marine (greenland cod, gadus ogac) fish from northern quebec, canada, were used to investigate the hypotheses that cestode parasites are (13)c and (15)n enriched relative to host food sources, but (15)n depleted with respect to host muscle tissue as a result of differential enrichment during the assimilation of common nutrient sources. cestode parasites and fish ...200415621744
polybrominated diphenyl ethers (pbdes) in marine fish and blue mussels from southern greenland.levels of polybrominated diphenyl ethers (pbdes) have not previously been reported in greenland. in this study shorthorn sculpins (myoxocephalus scorpius) were sampled at three locations in southern greenland; usuk (no population), igaliko (population 40) and qaqortoq (population 3200). furthermore uvak (gadus ogac), spotted wolffish (anarhichas minor), starry ray (raja radiata), and blue mussels (mytilus edulis) were collected at usuk. pooled samples of fish liver and blue mussel were analysed ...200212047074
antifreeze glycoproteins: relationship between molecular weight, thermal hysteresis and the inhibition of leakage from liposomes during thermotropic phase transition.antifreeze glycoproteins (afgp) were isolated and purified from the blood plasma of rock cod (gadus ogac), using deae-bio-gel ion exchange chromatography, followed by high performance liquid chromatography (hplc). the purified proteins were analyzed using polyacrylamide gel electrophoresis (page), and electrospray mass spectrometry. the results indicated that rock cod synthesize seven size classes of glycoproteins, ranging from 2.6 to 24 kda, with each size class containing multiple isoforms. an ...200111207440
does the ability to metabolically downregulate alter the hypoxia tolerance of fishes? a comparative study using cunner (t. adspersus) and greenland cod (g. ogac).in this study, the metabolic response and tolerance of cunner (a temperate wrasse species capable of metabolic depression) to graded hypoxia (water o(2) saturation 100-10%) was measured at two temperatures ( approximately 1 and 8 degrees c), and compared with that of the greenland cod (gadus ogac). cunner had significantly lower oxygen consumption (mo(2)) values at both 1 (21.2+/-2.4 mg o(2) kg(-0.83) hr(-1)) and 8 degrees c (31.6+/-1.5 mg o(2) kg(-0.83) hr(-1)) as compared with the greenland co ...200919235899
trypsin from greenland cod, gadus ogac. isolation and comparative properties.trypsin(ogen) was isolated from the pyloric ceca of greenland cod. greenland cod trypsin catalyzed hydrolysis of n alpha-benzoyl-dl-arginine p-nitroanilide, tosyl arginine methyl ester and protein and was inhibited by the serine protease inhibitor pmsf and other well-known trypsin inhibitors. greenland cod trypsin was more stable at alkaline ph than at acid ph; and was inactivated by relatively low thermal treatment. like other trypsins, the enzyme was rich in potential acidic amino acid residue ...19846518765
examination of fine-scale spatial-temporal overlap and segregation between two closely related congeners gadus morhua and gadus ogac in coastal newfoundland.the spatial and temporal movement patterns of sympatric juvenile atlantic cod gadus morhua and greenland cod gadus ogac were studied using high-resolution radio-acoustic positioning in a coastal area of newfoundland during the summers of 2009 and 2010. a total of 20 fish (10 g. ogac and 10 g. morhua) were equipped with acoustic transmitters and monitored for periods up to 23 days. most fishes showed high site fidelity with mean residence times of 12·4 (g. morhua) and 14·4 days (g. ogac). a few i ...201425041096
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