| distribution of the salmonid hpa 1 family in the salmonid species demonstrated by in vitro runoff transcription assay of total genomic dna: a procedure to estimate repetitive frequency and sequence divergence of a certain repetitive family with a few known sequences. | an in vitro runoff transcription assay of total genomic dna was developed. as an example of use of this assay, analysis of a highly repetitive sequence in the cherry salmon (oncorhynchus masou) is described. total genomic dna of the cherry salmon was completely digested with hpa 1, whose site is known to be in the trna-unrelated region of the cherry salmon hpa 1 family. on transcription of the digested dna in a hela cell extract, a discrete-sized rna of about 100 nucleotides, constituting 70% of ... | 1991 | 1707099 |
| a novel acid ribonuclease from white-spotted charr (salvelinus leucomaenis): purification and characterization of the homogeneous enzyme. | an novel acid ribonuclease has been purified approx. 1300 fold from acid extract of developing white-spotted charr (salvelinus leucomaenis) testes. the final preparation of the enzyme was homogeneous on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (sds). the molecular weight was determined to be 20,000 with both sephadex gel filtration and sds-polyacrylamide gel electrophoresis. the isoelectric point was determined to be 6.4 and the hydrolytic activity was stimula ... | 1983 | 6664849 |
| phylogeography of white-spotted charr (salvelinus leucomaenis) inferred from mitochondrial dna sequences. | the white-spotted charr (salvelinus leucomaenis) is a coldwater-adapted fish distributed in far-eastern asia. to assess phylogeographic patterns of this species over most of its range in the japanese archipelago and sakhalin island, russia, we examined nucleotide sequences of the mitochondrial dna (mtdna) cytochrome b region (557 bp) in 141 individuals from 50 populations. a total of 33 (5.5%) nucleotide positions were polymorphic and defined 29 haplotypes. phylogenetic analysis assigned the obs ... | 2004 | 14993836 |
| occurrence of deformed fish and their fitness-related traits in kirikuchi charr, salvelinus leucomaenis japonicus, the southernmost population of the genus salvelinus. | kirikuchi charr, salvelinus leucomaenis japonicus, is the southernmost population of the genus salvelinus. it is endemic to the kii peninsula, central honshu island, japan. as a consequence of anthropogenic disturbances, a few populations of kirikuchi charr with low genetic diversity now survive only in small, isolated habitats. this study investigated the occurrence of deformed individuals and assessed differences between deformed and nondeformed fish in fitness-related traits, i.e., body size, ... | 2006 | 16908958 |
| mitochondrial dna population structure of the white-spotted charr (salvelinus leucomaenis) in the lake biwa water system. | a phylogeographic analysis of mitochondrial dna sequences was performed in order to elucidate the origin, dispersal process, and genetic structure of white-spotted charr in the lake biwa water system. two haplotypes were most common in the lake biwa water system, and were also common in the adjacent inlet rivers of the sea of japan. these results suggest that in the glacial periods of the pleistocene, white-spotted charr dispersed into the northern inlet rivers of lake biwa from adjacent inlet r ... | 2008 | 18533745 |
| evidence for interspecific hybridization between native white-spotted charr salvelinus leucomaenis and non-native brown trout salmo trutta on hokkaido island, japan. | hybrids between native white-spotted charr salvelinus leucomaenis and non-native brown trout salmo trutta were identified in streams of hokkaido, japan, using both appearance and genetic characters. the dna analyses indicated that the specimens were hybrids between female s. leucomaenis and male s. trutta. occurrence of such hybrids implies increased mating opportunities between these species in wild streams. | 2009 | 20735572 |
| nematomorph parasites drive energy flow through a riparian ecosystem. | parasites are ubiquitous in natural systems and ecosystem-level effects should be proportional to the amount of biomass or energy flow altered by the parasites. here we quantified the extent to which a manipulative parasite altered the flow of energy through a forest-stream ecosystem. in a japanese headwater stream, camel crickets and grasshoppers (orthoptera) were 20 times more likely to enter a stream if infected by a nematomorph parasite (gordionus spp.), corroborating evidence that nematomor ... | 2011 | 21560690 |
| susceptibility of various japanese freshwater fish species to an isolate of viral haemorrhagic septicaemia virus (vhsv) genotype ivb. | genotype ivb of viral haemorrhagic septicaemia virus (vhsv) was isolated for the first time in the great lakes basin in 2003, where it spread and caused mass mortalities in several wild fish species throughout the basin. in order to prevent further spreading of the disease and to assess risks of new genotypes invading new watersheds, basic microbiological information such as pathogenicity studies are essential. in this study, experimental infections were conducted on 7 indigenous freshwater fish ... | 2013 | 24270018 |
| [phylogenetic relationships of sakhalin taimen parahucho perryi inferred from pcr-rflp analysis of mitochondrial dna]. | rflp analysis of three amplified mtdna fragments (cytb/d-loop, nd1/nd2, and nd3/nd4l/nd4) was performed in the following taxa: parahucho perryi, hucho taimen, brachymystax lenok, b. tumensis, salmo salar, salvelinus leucomaenis, and s. levanidovi. for mtdna of p. perryi, a substantial decrease in the haplotype and nucleotide diversity was observed as a result of random genetic drift, caused by a reduction in the effective population size. nucleotide divergence estimates between the mtdna haploty ... | 2008 | 18767536 |
| developing a food web-based transfer factor of radiocesium for fish, whitespotted char (salvelinus leucomaenis) in headwater streams. | we developed a food web-based transfer factor (tfweb) to study contaminant movements from multiple prey items to a predator based on the dietary contributions of prey items with their respective contamination levels. tfweb was used to evaluate the transfer of (137)cs into whitespotted char (salvelinus leucomaenis) from the trophic structure of a stream-riparian ecosystem in headwater streams draining a japanese cedar forest. we also examined the applicability of this method by comparing sites wi ... | 2017 | 28390308 |
| cloning, molecular characterization, and expression analysis of the signal transducer and activator of transcription 3 (stat₃) gene from grass carp (ctenopharyngodon idellus). | signal transducer and activator of transcription 3 (stat₃) binds to janus kinase 2 (jak₂) to initiate the jak₂/stat₃ signal transduction pathway, which plays an important role in cancer cell proliferation, immune regulation, reproduction, lipid metabolism, and other physiological processes of the organism. in this study, the cdna sequence of the stat₃ gene from grass carp was cloned using race (rapid-amplification of cdna ends). twelve characteristics of the stat₃ gene and its encoded protein se ... | 2013 | 24055509 |