| toxicity of natural pyrethrins and five pyrethroids to fish. | the toxicity of natural pyrethrins and five pyrethroids was determined with coho salmon (oncorhynchus kisutch), steelhead trout (salmo gairdneri), fathead minnow (pimephales promelas), channel catfish (icatlurus punctatus), bluegill (lepomis macrochirus), and yellow perch (perca flavescens). the 96-hour lc50's in static tests at 12 degrees c ranged from 24.6 to 114 mug/l of natural pyrethrins and from 0.110 to 1,140 mug/l of pyrethroids. two pyrethroids, ru-11679 and sbp-1382 (r), were over 10 t ... | 1976 | 5059 |
| ultrastructure of the thyroid gland in goitered coho salmon (oncorhynchus kisutch). | thyroid tissues in coho salmon (oncorhynchus kisutch) goiters showed marked regional variation in structure and ultrastructure. for the most part the follicles were small, were composed of tall epithelial cells, and contained little or no colloid material. the follicle epithelial cells were essentially similar in fine structure to hyperactive thyroids in mammals and one type of follicle in congenital goiters in humans. a second type of follicle, present in human goiters, was not found in coho sa ... | 1978 | 201370 |
| hypothyroidism in rats fed great lakes coho salmon. | | 1979 | 486781 |
| temperature and species differences in susceptibility of kidney cell cultures to mercury toxicity. | the effect of temperature on inorganic mercury toxicity was investigated using kidney tissue culture systems. the relative susceptibility of rabbit (homeothermic) kidney to mercury intoxication was compared to that of coho salmon (poikilothermic) kidney to mercury intoxication was compared to that of coho salmon (poikilothermic) kidney over temperature ranges consistent with the habitat of each of the two species. it was demonstrated that susceptibility to mercury toxicity is species dependent; ... | 1978 | 566720 |
| influence of bovine growth hormone on growth rate, appetite, and food conversion of yearling coho salmon (oncorhynchus kisutch) fed two diets of different composition. | | 1977 | 837283 |
| influence of combinations of bovine growth hormone, 17 alpha-methyltestosterone, and l-thyroxine on growth of yearling coho salmon (oncorhynchus kisutch). | | 1977 | 884630 |
| effects of fasting and feeding various diets on hepatic lipogenic enzyme activities in coho salmon (oncorhynchus kisutch (walbaum)). | the influence of fasting and diet composition on the time sequence of changes in hepatic lipogenic enzyme activities in coho salmon was investigated. young coho salmon fed a high-carbohydrate diet for 3 weeks were then fasted for 2, 6, or 23 days. liver preparations were assayed for fatty acid synthetase, citrate cleavage enzyme, malic enzyme, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities. fasting the fish for 2 or 6 days did not influence the activities of th ... | 1977 | 886388 |
| glochidiosis of salmonid fishes. i. comparative susceptibility to experimental infection with margaritifera margaritifera (l.) (pelecypoda: margaritanidae). | this is the first report on the susceptibility of salmonid fishes to infection with the glochidia of the freshwater mussel margaritifera margaritifera using known numbers of parasites under controlled conditions. the relative susceptibility of six species of salmonid fish, 20 to 80 mm in total length, to glochidiosis was determined by exposing fish individually to different numbers of parasites and plotting mortalities against these exposure levels at 70 days postexposure and also against the nu ... | 1977 | 886411 |
| binding proteins from fish sera and intrinsic factor compared in vitamin b12 radioassay. | we compare serum proteins from rainbow trout, chinook salmon, coho salmon, and oyster toadfish with intrinsic factor as binding proteins in a simplified radioassay for b12. regression analysis of b12 values, determined in 21 serum samples, shows good correlation (r greater than .975) between results for the fish sera and intrinsic factor. the accuracy of the five assays, as evaluated by analytical recovery of b12 added to pooled human serum, ranges from 90 to 110%. intra-assay precision ranges f ... | 1977 | 912869 |
| a preliminary study of insulin participation in the growth regulation of coho salmon (oncorhynchus kisutch). | | 1977 | 922614 |
| the epizootiology and pathogenesis of thyroid hyperplasia in coho salmon (oncorhynchus kisutch) in lake ontario. | the thyroid glands of coho salmon collected at different stages of their anadromous migration exhibited progressive and extensive hyperplasia and hypertrophy. the incidence of overt nodule formation rose from 5% in fish collected in august to 24% in fish collected in october. the histological picture of the goiters was similar to that found in thiourea-treated teleosts and thiouracil-treated mammals. there was a concomitant, significant decrease in serum thyroxine and triiodothyronine values bet ... | 1976 | 1000494 |
| rhabdochona kisutchi sp. nov. (nematoda: spiruroidea) from coho salmon, oncorhynchus kisutch (walbaum), of western canada. | | 1975 | 1139471 |
| a preliminary investigation of the effect of bovine growth hormone on growth and muscle composition of coho salmon (oncorhynchus kisutch). | | 1975 | 1205122 |
| molecular and genetic characterization of cytochrome oxidase-negative aeromonas salmonicida isolated from coho salmon (oncorhynchus kisutch). | cytochrome oxidase variants of the bacterial fish pathogen, aeromonas salmonicida, were characterized for genetic and molecular homology with cytochrome oxidase-positive isolates that typically induce furunculosis in salmonids. protein and lipopolysaccharide moieties of the cytochrome oxidase-negative variants were similar to their typical counterparts, based on sodium-dodecyl-sulfate polyacrylamide gel electrophoresis. pathogenicity of aberrant isolates to brook trout (salvelinus fontinalis) wa ... | 1992 | 1335520 |
| regulation of hepatic growth hormone receptors in coho salmon (oncorhynchus kisutch). | factors potentially regulating hepatic growth hormone (gh) receptors in coho salmon (oncorhynchus kisutch) have been investigated. from december to june of the first year, relative changes in hepatic 125i-sgh binding and 35so4 incorporation by ceratobranchial cartilage were similar. stunted salmon, which in seawater have elevated plasma gh yet fail to grow, showed lower hepatic 125i-sgh binding than did normally growing seawater salmon. however, mgcl2 treatment of stunts' membranes to reveal tot ... | 1992 | 1335940 |
| identification of insulin-like growth factor-binding proteins in the circulation of four teleost fish species. | insulin-like growth factor-binding proteins (igf-bps) were demonstrated in the circulation of four teleost fish species. in the coho salmon (oncorhynchus kisutch), serum binding of 125i-labelled human igf-i (125i-higf-i) was competitively inhibited by addition of excess recombinant bovine igf-i (rbigf-i) in a manner similar to that when rat serum was used. western-ligand blot procedure using the same labelled hormone identified at least three major forms of igf-bps in the plasma of all four tele ... | 1992 | 1380067 |
| stimulation of coho salmon growth by insulin-like growth factor i. | the effect of insulin-like growth factor i on growth rate of coho salmon (oncorhynchus kisutch) was examined. juvenile coho salmon received implants of osmotic minipumps containing recombinant bovine insulin-like growth factor i (rbigf-i) or saline for a period of 3 to 4 weeks. high doses of rbigf-i (greater than 0.13 microgram.g-1.d-1) resulted in hypoglycemia and death. in 2-year-old coho salmon, 0.09 microgram.g-1.d-1 rbigf-i administered for 25 days doubled linear growth rate and increased g ... | 1992 | 1398004 |
| nucleotide sequence and tissue distribution of three insulin-like growth factor i prohormones in salmon. | tissue distribution and potential alternative splicing of insulin-like growth factor i (igf-i) messenger rna were studied using reverse transcriptase-polymerase chain reaction (rt-pcr) on rna from several tissues at various stages of the life cycle of coho salmon (oncorhynchus kisutch). dna sequence analysis of rt-pcr products revealed three igf-i mrna transcripts, designated ea-1, ea-2, and ea-3, which code for three distinct prohormones, igf-ia-1, igf-ia-2, and igf-ia-3, respectively. the e-do ... | 1992 | 1406698 |
| identification of a second insulin-like growth factor in a fish species. | an internal portion of insulin-like growth factor (igf) amplified from the total cdna of rainbow trout (oncorhynchus mykiss) liver by a pcr was used to screen a rainbow trout liver cdna library, and recombinant clones encoding two distinct igfs were isolated. on the basis of a 98.7% nucleotide and 98.3% predicted amino acid identity to coho salmon igf-i, one cdna sequence was identified as rainbow trout preproinsulin-like growth factor i (rtigf-i). the second cdna sequence shared 46.1% and 43.3% ... | 1992 | 1409585 |
| chum salmon (oncorhynchus keta) stanniocalcin inhibits in vitro intestinal calcium uptake in atlantic cod (gadus morhua). | chum salmon (oncorhynchus keta) stanniocalcin was purified, partially identified and tested for bioactivity in an assay on the intestinal calcium uptake in a marine teleost (gadus morhua). basic ethanol extraction, ion exchange chromatography, gel filtration and reverse-phase high-performance liquid chromatography resulted in the isolation of a homogeneous glycoprotein that appears as a 46-kda product under nonreducing conditions and as a 23-kda product under reducing conditions after sodium dod ... | 1992 | 1430418 |
| characterization of peptides related to neuropeptide tyrosine and peptide tyrosine-tyrosine from the brain and gastrointestinal tract of teleost fish. | neuropeptide y was isolated from the brain of the atlantic cod, gadus morhua and its primary structure established as tyr-pro-ile*-lys-pro-glu*-asn-pro-gly-glu10-asp-ala-pro-ala-asp*-g lu*-leu*-ala- lys*-tyr20-tyr-ser-ala-leu-arg-his-tyr-ile-asn-leu30-ile-thr -arg-gln-arg-tyr- conh2. residues denoted by an asterisk are different from the corresponding sequence of human neuropeptide y. a structurally similar peptide was isolated from the brain of the trout, oncorhynchus mykiss. trout neuropeptide ... | 1992 | 1459125 |
| mass mortality in cultured coho salmon (oncorhynchus kisutch) due to saprolegnia parasitica coker. | epizootics of saprolegniasis occurred in 20 to 60 g freshwater-cultured coho salmon (oncorhynchus kisutch) in miyagi prefecture, japan. cotton-like mycelia occurred on the body surface of infected fish, especially around the head, the adipose fin and the caudal fin, and aseptate hyphae occurred in the lesions. the hyphae also penetrated into the muscle and blood vessels. the isolated fungus was identified by asexual morphological characteristics as saprolegnia parasitica (syn. s. diclina type 1) ... | 1992 | 1474649 |
| asynchrony of changes in tissue and plasma thyroid hormones during the parr-smolt transformation of coho salmon. | the relationship between plasma thyroid hormone concentrations and the thyroid hormone concentrations in selected tissues was examined throughout the spring during the typical course of parr-smolt transformation in coho salmon (oncorhynchus kisutch) in fresh water and also in coho salmon moved prematurely to seawater. the thyroid hormones thyroxine (t4) and triiodothyronine (t3) were extracted from brain, liver, and muscle tissue. the t4 and t3 concentrations in the extracts and plasma were meas ... | 1992 | 1490585 |
| detection of renibacterium salmoninarum antigen in migrating adult chum salmon (oncorhynchus keta) in japan. | renibacterium salmoninarum antigen was detected in the kidney of migrating chum salmon (oncorhynchus keta) using the indirect dot blot assay and indirect fluorescent antibody test. the adult chum salmon had migrated into a bay in which cultured coho salmon infected with r. salmoninarum were present. antigen was detected in 5% of the chum salmon although they did not have clinical signs of bacterial kidney disease (bkd). this report describes the first case of r. salmoninarum antigen detection am ... | 1992 | 1548789 |
| hormonal control of sulfate uptake by branchial cartilage of coho salmon: role of igf-i. | the direct hormonal control of sulfate uptake by cartilage matrix of coho salmon was examined by exposing branchial cartilage to 1 microci.ml-1 35so4 for 48 hours at 15 degrees c in a defined medium. sulfate uptake occurred primarily in cartilage (rather than bone) and the amount of specific uptake was similar in epibranchial and ceratobranchial cartilages. intact and hypophysectomized coho salmon starved for 22 days had equivalent in vitro sulfate uptake, which in both cases were 30% of the upt ... | 1992 | 1583459 |
| effects of insulin-like growth factor-i and insulin on the in-vitro uptake of sulphate by eel branchial cartilage: evidence for the presence of independent hepatic and pancreatic sulphation factors. | the possible roles of insulin-like growth factor-i (igf-i) and insulin in regulating cartilage growth were studied in the teleost anguilla japonica. significant sulphation activity was found in the extracts of pancreas, liver and muscle, but not in those of kidney, intestine or spleen. the hepatic sulphation activity was significantly decreased by hypophysectomy or by fasting for 14 days, suggesting that this activity is regulated by pituitary function and nutritional status. northern blot analy ... | 1992 | 1613424 |
| histochemical and immunocytochemical identification of the pituitary cell types in three sciaenid fishes: atlantic croaker (micropogonias undulatus), spotted seatrout (cynoscion nebulosus), and red drum (sciaenops ocellatus). | the adenohypophysial cell types in three species of sciaenid teleosts were identified using a combination of classical histochemical techniques and immunocytochemistry with antisera raised against piscine and human pituitary hormones. in general greater specificity for the different cell types was observed with antisera to the piscine pituitary hormones than with antisera raised against the human hormones. the distribution of the cell types did not differ significantly among the three species, a ... | 1991 | 1667000 |
| radioimmunoassay for salmon pancreatic somatostatin-25. | a specific and sensitive radioimmunoassay (ria) for the measurement of plasma levels of somatostatin-25 (ss-25) in salmon was developed using antisera raised against coho salmon (oncorhynchus kisutch) ss-25. somatostatin-25 was iodinated by the chloramine-t method and repurified on sephadex g-25. the ria was performed using a double antibody (goat anti-rabbit gammaglobulin as second antibody) method under disequilibrium conditions. plasma from several salmonids (coho, chinook, rainbow trout, bro ... | 1991 | 1676008 |
| development and validation of a competitive enzyme immunoassay for chum salmon prolactin: a comparison to radioimmunoassay. | an enzyme immunoassay (eia), based on a competitive assay system, for the measurement of prolactin (prl) in the pituitary of salmonid fishes and of hormone released in medium from incubated pituitary was developed using a rabbit antiserum to chum salmon prl (prl, a combination of prl i and prl ii). chum salmon prl was coupled to horseradish peroxidase (hrp). the incubation procedure for the antigen-antibody reaction was analogous to that in the radioimmunoassay (ria) for prl. the antibody-bound ... | 1990 | 1705233 |
| isolation and phenotypic characterization of an oxidase-negative aeromonas salmonicida causing furunculosis in coho salmon (oncorhynchus kisutch). | an oxidase-negative aeromonas salmonicida was isolated from coho salmon (oncorhynchus) kisutch) suffering from an epizootic of furunculosis at the state hatchery near belfair, washington. typical, oxidase-positive a. salmonicida was isolated concurrently from the same population of fish. mortality was controlled with medicated feed treatments. evidence supporting the identification of the two types of a. salmonicida is presented. methods for the proper identification of oxidase-negative a. salmo ... | 1991 | 1850808 |
| isolation and characterization of two coho salmon gonadotropins, gth i and gth ii. | two gonadotropins, gth i and gth ii, were isolated from pituitaries of spawning coho salmon (oncorhynchus kisutch) using sequential extractions with ammonium acetate (ph 9.0) and 40% ethanol, precipitation with 80% ethanol, gel filtration chromatography (sephadex g-100), anion-exchange chromatography (mono-q sepharose), and gel filtration chromatography (sephadex g-75). coho salmon gth i and gth ii stimulated steroidogenesis in vitro in a similar dose-dependent manner when incubated with either ... | 1991 | 2015351 |
| cortisol effects on plasma electrolytes and thyroid hormones during smoltification in coho salmon oncorhynchus kisutch. | plasma concentrations of na+, k+, triiodothyronine (t3), and thyroxine (t4) and muscle water content were measured in yearling coho salmon. oncorhynchus kisutch, after injection of cortisol in april, may, and june in fresh water (fw) and during acclimation to seawater (sw). cortisol (17-21 micrograms/g), injected intraperitoneally in a melted cocoa butter suspension, suppressed the rise of plasma na+ during sw acclimation in april but not in may or june. muscle water content increased during sw ... | 1991 | 2055437 |
| the epithelial neoplasm observed in the cultured coho salmon, oncorhynchus kisutch. | the epithelial neoplasms were observed on the mouth of the cultured coho salmon, oncorhynchus kisutch. histopathologically, the tumors were formed to be proliferative epithelial cells, but no change was observed in other organs. the virus from this tumor was isolated in rtg-2 and chse-214 cells and developed the cytopathic effect which characterized to be the formation of syncytia and the migration of chromatin. this virus was neutralized with anti-oncorhynchus masou virus (omv) rabbit serum. | 1990 | 2096259 |
| immunological detection of in vivo aflatoxin b1-dna adduct formation in rats, rainbow trout and coho salmon. | immunological detection of carcinogen-dna adducts in organs or tissues should prove a particularly useful approach for monitoring carcinogen exposure, for characterization of carcinogen binding to dna and for investigating dna repair processes in vivo. in one of a series of experiments aimed at raising antibodies against several carcinogen-modified dnas, rabbits were immunized in our laboratory with aflatoxin b1 (afb1)-modified dna. after the titer and specificity of the antibodies produced were ... | 1990 | 2119257 |
| [parasitism in fishes and human riverside communities of the huillinco and natri lakes (great island of chiloé), chile]. | in april and december 1989, 35 fish from lake huillinco (42 degrees 48's, 74 degrees 02'w) and 36 fish from lake natri (42 degrees 48's, 73 degrees 50'w), in the great island of chiloé (chile) were examined. coprological samples from 159 persons, 17 dogs, 19 pigs and 4 cats from around both lakes were examined for diphyllobothrium spp. infection. in the lake huillinco the following helminths of fishes were determined: contracaecum sp. and hysterothylacium sp. in salmo trutta, cauque mauleanum an ... | 1990 | 2152358 |
| restriction endonuclease fingerprinting analysis of canadian isolates of aeromonas salmonicida. | restriction endonuclease fingerprinting (ref) analysis was used to examine total cellular dna prepared from 56 independent field isolates of the fish pathogen, aeromonas salmonicida. dna was digested singly with the restriction enzymes ecori and hindiii, and the resulting fragments separated by polyacrylamide gel electrophoresis and visualized by silver staining. the ref patterns of typical isolates of a. salmonicida subsp. salmonicida were distinct from those of a. hydrophila, a. salmonicida su ... | 1990 | 2159364 |
| regulation of oogenesis: the piscine receptor for vitellogenin. | the receptor-mediated uptake of vitellogenin (vtg), a plasmatic lipophosphoglycoprotein, is crucial for oocyte growth in egg-laying animals. the plasma membrane receptor for vtg was characterized from oocytes of coho salmon, oncorhynchus kisutch. in direct binding studies, the receptor exhibited high affinity (kd, 180 nm) for salmonid vtg, and by ligand blotting with radiolabelled vtg it was visualized as a protein with an apparent mr of 100,000, under non-reducing conditions. the fish vtg recep ... | 1990 | 2167133 |
| salmonid pituitary gonadotrophs. i. distinct cellular distributions of two gonadotropins, gth i and gth ii. | using antisera specific for the beta subunits of two distinct coho salmon gonadotropins, gth i and gth ii, an immunocytochemical study of rainbow trout and atlantic salmon pituitaries was done. cells which immunostained with anti-gth i beta were distributed in the periphery of the glandular cords of the proximal pars distalis (ppd), in close association with somatotrophs. on the other hand, cells immunostained with anti-gth ii beta were located in the central parts of the glandular cords of the ... | 1990 | 2186958 |
| purification and characterization of atlantic salmon growth hormone and evidence for charge heterogeneity. | highly purified growth hormone (gh) has been isolated from atlantic salmon (salmo salar) pituitaries by extraction with acid acetone, acidic precipitation, and reversed-phase high-performance liquid chromatography (hplc). the yield was 2.5 mg/g wet tissue. the atlantic salmon gh (sgh) emerged as a single symmetrical peak after hplc on a reverse phase c18 column. sds-gel electrophoresis revealed only one band with an estimated molecular weight of 23,000. atlantic sgh showed a uniform molecular we ... | 1990 | 2289675 |
| human nanophyetiasis: transmission by handling naturally infected coho salmon (oncorhynchus kisutch). | the first us case of human nanophyetiasis that does not involve ingestion of raw or undercooked salmonid fishes is reported. the patient worked with highly infected fish. hand contamination with the infectious metacercariae of the digenetic trematode nanophyetus salmincola (family troglotrematidae) occurred during the handling of fresh-killed, juvenile coho salmon, oncorhynchus kisutch. diagnosis of nanophyetiasis was based on the clinical findings of chronic diarrhea, nausea, abdominal discomfo ... | 1990 | 2295848 |
| comparative studies of catalase and superoxide dismutase activity within salmon fish erythrocytes. | 1. superoxide dismutase isolated from erythrocytes of several species of salmon and the rainbow trout exhibited single electrophoretic bands of activity which migrated anodally similar to the human erythrocyte enzyme; two discrete bands were observed for the coho salmon. 2. no polymorphism was observed for 30 samples from sockeye salmon and six samples from king salmon. only one sample of rainbow trout (one of 12) exhibited an electrophoretic mobility difference. 3. catalase migration on starch- ... | 1990 | 2331875 |
| experimental transmission of a plasmacytoid leukemia of chinook salmon, oncorhynchus tshawytscha. | a plasmacytoid leukemia of chinook salmon, oncorhynchus tshawytscha, has recently been recognized in seawater netpens in british columbia, canada. the disease has occurred at several sites and has caused high mortality. plasmacytoid leukemia is characterized by a generalized invasion of visceral tissues and the orbit of the eye by plasmacytoid cells. the disease was experimentally transmitted to healthy chinook salmon by i.p. injection of kidney tissue homogenates, but transmission with a cell-f ... | 1990 | 2386969 |
| lunar cycles of coho salmon, oncorhynchus kisutch. ii. scale amino acid uptake, nucleic acids, metabolic reserves and plasma thyroid hormones. | [14c]glycine uptake by scales in vitro was measured in coho salmon (oncorhynchus kisutch walbaum) smolts at different times during several semi-lunar cycles. there was a clear cyclical pattern of glycine uptake during the semi-lunar period. evidence for semi-lunar cycles of liver and muscle rna:dna ratios, carcass water content, haematocrit, and plasma triglyceride, glucose and cholesterol levels was also found in coho salmon parrs. plasma l-thyroxine (t4) levels exhibited a cyclical pattern dur ... | 1987 | 2438366 |
| parathyroid hormone-like immunoreactivity in fish plasma and tissues. | although fish lack parathyroid glands, parathyroid hormone (pth)-like substances, reacting with guinea pig antibodies raised against bovine pth1(-84), were detected in the circulation of trout (salmo gairdneri) and goldfish (carassius auratus). the immunoreactive (ir) pth concentration in plasma (0.5-1.5 ng/ml) was less than that measured in heat-treated and dialyzed 0.6% nacl extracts of brain tissue (4.0-5.0 ng/g wet wt brain) and much less than that present in pituitary extracts (20-40 ng/g p ... | 1987 | 2444491 |
| the primary structure of coho salmon growth hormone and its cdna. | total rna was extracted from coho salmon growth hormone (sgh) cell regions and used to synthesize double-stranded cdna, which was inserted into a plasmid vector and used to transform escherichia coli hb101. the total rna was also separated according to size by electrophoresis on agarose gels and the fraction that directed the cell-free synthesis of protein in the size range of ghs of other species was isolated and used to screen the transformed colonies of e. coli. a clone containing the putativ ... | 1987 | 2449377 |
| autonomic innervation of the spleen of the coho salmon, oncorhynchus kisutch. a histochemical demonstration and preliminary assessment of its immunoregulatory role. | regulation of immunity by the nervous system, now a well-established phenomenon in mammals, is effected in part through the autonomic innervation of lymphoid tissues. noradrenergic fibers specifically target lymphocyte-rich areas in mammalian lymphoid tissues, and their ablation, or the administration of adrenergic agents, can significantly alter immune responses. this study demonstrates that the spleen of the coho salmon is also richly innervated by adrenergic neurons. while this innervation en ... | 1989 | 2514816 |
| immunomodulation in c57bl/6 mice following consumption of halogenated aromatic hydrocarbon-contaminated coho salmon (oncorhynchus kisutch) from lake ontario. | this report describes studies designed to assess the immunomodulatory effects associated with the consumption of coho salmon containing halogenated aromatic hydrocarbons (hahs) and other compounds naturally bioaccumulated from lake ontario. diets containing 33% coho salmon from lake ontario or the pacific ocean were fed to juvenile c57bl/6 mice for 2-4 mo. following 60 d, the mice that consumed lake ontario salmon had reduced igm, igg, and iga plaque-forming cell responses to sheep erythrocytes. ... | 1989 | 2527307 |
| metabolic changes in coho and chinook salmon resulting from acute insufficiency in pancreatic hormones. | acute deficiency in pancreatic peptides (insulin, somatostatin-25, glucagon, and glucagon-like peptide) was invoked for 9-12 hr in coho, oncorhynchus kisutch, and chinook, o. tshawytscha, salmon by administration of specific antisera raised against purified salmon hormones. insulin-deficient fish were hyperglycemic, had diminished glycogen content in the liver (plisetskaya et al., '88a, elevated liver triacylglycerol lipase activity, and higher concentration of plasma triiodothyronine (t3) compa ... | 1989 | 2566643 |
| comparative sensitivities of diagnostic procedures used to detect bacterial kidney disease in salmonid fishes. | kidney and spleen homogenates from each of 60 coho salmon (oncorhynchus kisutch) and steelhead trout (salmo gairdneri) were examined for detection of renibacterium salmoninarum. the proportions of positives differed widely with the detection procedures used: in coho salmon, 5% were positive by the gram-stain procedure, 10% by the direct fluorescent antibody test, 48% by bacteriological isolation, 65% by staphylococcal coagglutination, and 73% by counterimmunoelectrophoresis; in steelhead trout, ... | 1985 | 2582153 |
| nucleotide sequence and growth hormone-regulated expression of salmon insulin-like growth factor i mrna. | protein and cdna sequence analysis have revealed that the insulin-like growth factor (igf-i) has been highly conserved among several mammalian species. using the combined techniques of polymerase chain reaction and molecular cloning, we have now obtained the cdna sequence encoding preproigf-i from a teleost species, oncorhynchus kisutch (coho salmon). the 2020 nucleotide (nt) cloned cdna sequence contains a 528 nt open reading frame encoding 176 amino acids in preproigf-i and 175 nt and 1317 nt ... | 1989 | 2628735 |
| metabolic effects of salmon glucagon and glucagon-like peptide in coho and chinook salmon. | different doses of glucagon and glucagon-like peptide (glp) isolated from coho salmon, oncorhynchus kisutch were tested in vivo and in vitro on juvenile coho and chinook (o. tshawytscha) salmon. results obtained suggest an involvement of these peptides in the regulation of plasma glucose, plasma fatty acids, liver glycogen, and the hepatic enzymes: glycogen phosphorylase, pyruvate kinase, triacylglycerol lipase, and glucose-6-phosphate dehydrogenase. metabolic effects were more enhanced in summe ... | 1989 | 2651208 |
| toxicology of chemical mixtures: experimental approaches, underlying concepts, and some results. | the toxicology of chemical mixtures will be the toxicology of the 1990s and beyond. while this branch of toxicology most closely reflects the actual human exposure situation, there is yet no standard protocol or consensus methodology for investigating the toxicology of mixtures. thus, in this emerging science, experimentation is required just to develop a broadly applicable evaluation system. several examples are discussed to illustrate the different experimental designs and the concepts behind ... | 1989 | 2690403 |
| comparative oxygen affinity of fish and mammalian myoglobins. | myoglobins from rat, coho salmon (oncorhynchus kisutch), buffalo sculpin (enophrys bison) hearts, and yellowfin tuna (thunnus albacares) red skeletal muscle were partially purified and their o2 binding affinities determined. commercially prepared sperm whale myoglobin was employed as an internal standard. tested at 20 degrees c, myoglobins from salmon and sculpin bound o2 with lower affinity than myoglobins from the rat or sperm whale. oxygen binding studies at 12 degrees c and 37 degrees c sugg ... | 1989 | 2760286 |
| prevalence of larval anisakis simplex in pen-reared and wild-caught salmon (salmonidae) from puget sound, washington. | the abundance of parasites of public health significance in pen-reared salmon and wild-caught salmon was compared. two hundred eighty-seven salmon from puget sound, washington, were examined for third-stage larvae of anisakis simplex. of these fish, 237 atlantic salmon (salmo salar), coho salmon (oncorhynchus kisutch) and chinook salmon (o. tshawytscha) were reared in commercial salmon pens and 50 sockeye salmon (o. nerka) were caught during their spawning migration. all wild-caught salmon were ... | 1989 | 2761015 |
| an homologous radioimmunoassay for coho salmon (oncorhynchus kisutch) vitellogenin, with general applicability to other pacific salmonids. | this paper describes an homologous radioimmunoassay for coho salmon vitellogenin that demonstrates parallel cross-reactivity for plasma vitellogenin of all pacific salmonids tested (chinook, chum, coho, pink, and sockeye salmon, and cutthroat and rainbow trout), but not for atlantic salmon or two nonsalmonids: common carp and sablefish. plasma vitellogenin levels were high in ovulatory female pacific salmonids (micrograms/ml to mg/ml range), but were mostly nondetectable in spermiating males of ... | 1989 | 2767411 |
| relationship between urotensin ii- and somatostatin-immunoreactive spinal cord neurons of catostomus commersoni and oncorhynchus kisutch (teleostei). | this immunocytochemical study describes the presence of separate immunoreactive (ir)-urotensin ii (uii) and ir-somatostatin (som) systems in the spinal cord of two species of teleost fish. both systems are arranged in a close spatial interrelationship in which ir-som fibres apparently innervate cerebrospinal fluid (csf)-contacting ir-uii neurons. specimens of oncorhynchus kisutch also display csf-contacting ir-som neurons located in the lateral ependymal walls of the central canal, in addition t ... | 1988 | 2906818 |
| pinealocytes immunoreactive with antisera against secretory glycoproteins of the subcommissural organ: a comparative study. | by means of light-microscopic immunocytochemistry two polyclonal antibodies (afru, aso; see p. 470) directed against secretory glycoproteins of the subcommissural organ were shown to cross-react with cells in the pineal organ of lamprey larvae, coho salmon, a toad, two species of lizards, domestic fowl, albino rat and bovine (taxonomic details, see below). the afru-immunoreactive cells were identified as pinealocytes of the receptor line (pineal photoreceptors, modified photoreceptors or classic ... | 1988 | 2976614 |
| effects of cortisol and growth hormone on osmoregulation in pre- and desmoltified coho salmon (oncorhynchus kisutch). | salmonid species which undergo smoltification show a concurrent enhancement in saltwater (sw) osmoregulatory ability. this developmental change is marked by an increase in sw tolerance and gill na+,k+-atpase activity which appears to result, in part, from an increase in gill chloride cell density. previous studies have suggested that cortisol and growth hormone (gh) may stimulate sw osmoregulatory mechanisms in salmonids. in this study, these hormones were examined for their ability to induce sm ... | 1987 | 3028904 |
| effects of cortisol and growth hormone replacement on osmoregulation in hypophysectomized coho salmon (oncorhynchus kisutch). | both cortisol and gh were able to reverse partially the effects of hypophysectomy on coho salmon (oncorhynchus kisutch) as judged by improved seawater (sw) tolerance after long-term treatment; however, neither hormone significantly restored gill na+, na+,k+-atpase activity. in the first experiment, gill na+,k+-atpase activity remained low in hypophysectomized (hx) coho treated with cortisol (15 micrograms/g body wt, suspended in cocoa butter) for 96 hr (48 hr in fresh water followed by 48 hr in ... | 1987 | 3040518 |
| colocalization of glucagon-like peptide and glucagon immunoreactivities in pancreatic islets and intestine of salmonids. | pancreatic islets of salmon contain at least two peptides of the glucagon family: 29-amino acid glucagon and 31-amino acid glucagon-like peptide (glp). both peptides were recently isolated from the pancreatic islets of coho salmon and sequenced (plisetskaya et al. 1986). antibodies generated against these two peptides and against human glucagon were used as immunocytochemical probes to investigate whether glucagon and glp are processed in the same, or in different cell types in the pancreatic is ... | 1988 | 3044602 |
| glucagon-like peptides activate hepatic gluconeogenesis. | piscine (anglerfish, catfish, coho salmon) glucagon-like peptides (glps), applied at 3.5 nm, stimulate (1.1-1.9-fold) flux through gluconeogenesis above control levels in isolated trout and salmon hepatocytes. human glp-1 and glp-2 also activate gluconeogenesis, but to a lesser degree than their piscine counterparts. minor increases of substrate oxidation are noticed at times of peak gluconeogenic activation through glps. these hormones, which are derived from the same precursor peptide as gluca ... | 1987 | 3109952 |
| the significance of glutathione conjugation for aflatoxin b1 metabolism in rainbow trout and coho salmon. | rainbow trout are known to be more susceptible to aflatoxin b1 (afb1) hepatocarcinogenesis than coho salmon, or trout pre-fed the carcinogenesis inhibitors beta-naphthoflavone (beta nf), aroclor 1254 or indole-3-carbinol. the study reported here examined the relationship between afb1-glutathione (gsh) conjugation and afb1 carcinogenesis in salmon, trout and trout pre-fed the three inhibitors. the afb1-glutathione (afb1-sg) conjugate was not detected in salmon bile and was present in trout bile i ... | 1988 | 3130299 |
| comparative biochemistry and physiology of teleocalcin from sockeye and coho salmon. | this is a comparative study of the glycoprotein hormone, teleocalcin, from the corpuscles of stannius of sockeye (oncorhynchus nerka) and coho (o. kisutch) salmon. coho teleocalcin was purified by the same procedures used previously to obtain sockeye teleocalcin and was obtained in a comparable yield. both salmon teleocalcins had the same molecular weight as estimated by sodium dodecyl sulfate-electrophoresis and both appeared to have the structure of disulfide-linked oligomers. the two hormones ... | 1988 | 3197944 |
| [parasitism by pleistophora sp. in coho salmon (oncorhynchus kisutch) in chile]. | | 1988 | 3269733 |
| vibrio anguillarum antigen stimulates mitogenesis and polyclonal activation of salmonid lymphocytes. | an antigen preparation of vibrio anguillarum, a salmonid pathogen, acts as a potent in vitro mitogenic stimulator of splenic and pronephric (anterior kidney) lymphocytes from coho salmon (oncorhynchus kisutch), chinook salmon (o. tshawytscha) and rainbow trout (salmo gairdneri). this antigen (va) is comparable in its mitogenic activity to concanavalin a (con a), escherichia coli lipopolysaccharide (lps), and phytohemagglutinin (pha). va gives peak mitogenic responses in coho five days after init ... | 1987 | 3315760 |
| thyrotropic activity of salmon pituitary glycoprotein hormones in the hawaiian parrotfish thyroid in vitro. | the thyrotropic activities of salmon pituitary extract, thyroid-stimulating hormone (tsh), gonadotropins (gth), and glycoprotein fractions obtained during purification of salmon tsh and gth were measured using the parrotfish thyroid culture system. purified salmon tsh was approximately 1,000 times more potent than bovine tsh in stimulating thyroxine release into the culture medium. most of the forms of salmon gth had no thyrotropic activity. one of the forms of salmon gth (gth-f) and three chrom ... | 1988 | 3367124 |
| variation in thyroid response to thyroid-stimulating hormone in juvenile coho salmon (oncorhynchus kisutch). | changes in the circulating levels of thyroid hormones measured by radioimmunoassay in yearling coho salmon in response to injection of bovine thyroid-stimulating hormone (tsh) were evaluated from january through august. doses of tsh ranging from 0.2 to 6.4 micrograms/g body wt were ineffective in stimulating t4 in january and at one time in april. responses to tsh injection at this dose range were observed beginning in february and continuing through august. the minimal effective dose of tsh was ... | 1987 | 3436520 |
| salmonid b lymphocytes demonstrate organ dependent functional heterogeneity. | the passive hemolytic plaque assay was used to examine the functional heterogeneity of antibody producing cells in salmonid immune organs. in this study, the antibody response to vibrio anguillarum antigens was induced by the injection of a somatic antigen extract. this antigen was also coated onto sheep red blood cells (srbc) for plaque forming cell (pfc) determination. previous studies have demonstrated that this response is antibody dependent and antigen specific (kaattari and irwin, 1985). t ... | 1986 | 3490046 |
| structure of a peptide from coho salmon endocrine pancreas with homology to neuropeptide y. | the amino acid sequence of a peptide isolated from the pacific salmon (oncorhynchus kisutch) endocrine pancreas has been determined. this simple 36 residue peptide is a member of the pancreatic polypeptide family. it contains a c-terminal tyrosinamide and is more homologous with porcine neuropeptide y (npy) (83%) and peptide yy (75%) than any of the previously characterized pancreatic polypeptides (pp). this peptide appears to be the major but not the only representative of this family of peptid ... | 1986 | 3545195 |
| pituitary thyrotropin and gonadotropin of coho salmon (oncorhynchus kisutch): separation by chromatofocusing. | thyrotropin (tsh) and gonadotropin (gth) were isolated from adult female coho salmon pituitary glands. after final extraction in acidic alcohol and precipitation in 85% ethanol, proteins were fractionated using gel filtration chromatography and chromatofocusing. homologous bioassay systems were used to monitor bioactivity during the purification procedures. tsh activity was measured in vivo in coho salmon parr. gth (steroidogenic) activity was determined in vitro using cultures of adult coho sal ... | 1987 | 3545978 |
| effects of thyroxin, cortisol, growth hormone, and prolactin on lipid metabolism of coho salmon, oncorhynchus kisutch, during smoltification. | juvenile coho salmon, oncorhynchus kisutch, were either immersed in thyroxin-containing water (t4; 20 micrograms/ml) or implanted with cortisol (5 mg), bovine growth hormone (gh; 1.5 microgram/g body wt), or ovine prolactin (prl; 1.5 microgram/g body wt), both early and late in smoltification. t4 and cortisol treatment stimulated lipid mobilization in parr. t4-stimulated lipid mobilization was indicated by decreased total lipids, primarily as triacylglycerols, and increased lipolytic enzyme (tri ... | 1986 | 3557090 |
| thyroid hormones in blood plasma of developing salmon embryos. | blood plasma thyroxine (t4) and 3,5,3'-triiodo-l-thyronine (t3) concentrations in developing embryos of chum (oncorhynchus keta), coho (oncorhynchus kisutch), chinook (oncorhynchus tschawytscha), and atlantic salmon (salmo salar) were measured by radioimmunoassay between hatching and completion of yolk-sac absorption. blood sampling was initiated when approximately 50% of the yolk that was present at hatching had been absorbed. at this time blood plasma levels of t4 (7-9 ng/ml) and t3 (1-5 ng/ml ... | 1987 | 3557098 |
| toxic effects in c57b1/6 and dba/2 mice following consumption of halogenated aromatic hydrocarbon-contaminated great lakes coho salmon (oncorhynchus kisutch walbaum). | diets containing coho salmon (oncorhynchus kisutch walbaum) from the pacific ocean or from lakes erie, michigan, and ontario [containing a gradation from low to high of halogenated aromatic hydrocarbons, (hahs)] were fed to c57b1/6 and dba/2 mice. following a 4-month dietary exposure to lake ontario salmon, both strains of mice demonstrated hepatomegaly. the ethoxyresorufin-o-deethylase (err) enzyme levels were elevated in livers of c57b1/6 mice fed diets of salmon from all of the great lakes st ... | 1987 | 3691436 |
| characterization of coho salmon (oncorhynchus kisutch) insulin. | insulin has been isolated from islet tissue of coho salmon (oncorhynchus kisutch) by gel filtration and hplc and the complete amino acid sequence has been determined. the sequence differs from bovine insulin at 14 sites but all interchanges are conservative from the viewpoint of preservation of conformation. a comparison of insulin sequences from other fish is presented. salmon insulin cross-reacts very weakly with antiserum to bovine insulin and vice versa. a completely homologous radioimmunoas ... | 1985 | 3898237 |
| salmonid spleen and anterior kidney harbor populations of lymphocytes with different b cell repertoires. | an o-antigen extract of the fish pathogen vibrio anguillarum was used to stimulate plaque-forming cells (pfc) in coho salmon. the kinetics of the response demonstrates peak pfc per organ on day 16 post immunization for the spleen and anterior kidney. significant pfc were also seen in thymic tissue. pfc responses were shown to be immunoglobulin mediated and antigen specific. inhibition profiles of responding pfc populations demonstrate that the cells of the anterior kidney are more restricted in ... | 1985 | 3899761 |
| studies on n alpha-acylated proteins: the n-terminal sequences of two muscle enolases. | a standard procedure for the identification of the n-terminal amino acid in n alpha-acylated proteins has been developed. after exhaustive proteolysis, the amino acids with blocked alpha-amino groups are separated from positively charged, free amino acids by ion exchange chromatography and subjected to digestion with acylase i. amino acid analysis before and after the acylase treatment identifies the blocked n-terminal amino acid. a survey of acylamino acid substrates showed that acylase will li ... | 1985 | 3913471 |
| dna homologies between the rainbow trout, chum salmon and coho salmon. | the thermal stabilities of hybrid duplexes between the dnas from three salmonid fish species were monitored as measures of dna homology. the chum salmon, oncorhynchus keta, and coho salmon, oncorhynchus kisutch, had more dna homology with each other than either had with the rainbow trout, salmo gairdnerii. morphological, ecological and protein similarities between the coho salmon and the rainbow trout may be due to parallel or convergent evolution. | 1985 | 4017549 |
| development and validation of a salmon prolactin radioimmunoassay. | a highly specific radioimmunoassay (ria) for the measurement of prolactin (prl) in the plasma and pituitary of salmonid fishes was developed using a rabbit antiserum to chinook salmon (oncorhynchus tschawytscha) prl. the prls purified from chinook salmon and chum salmon (o. keta) pituitaries showed exactly the same competitive inhibition curves in the ria, regardless of iodination of either hormone. the displacement curves for pituitary extracts and plasma from several salmonids, including chum, ... | 1985 | 4018564 |
| immunity to aeromonas salmonicida in coho salmon (oncorhynchus kisutch) induced by modified freund's complete adjuvant: its non-specific nature and the probable role of macrophages in the phenomenon. | juvenile coho salmon (oncorhynchus kisutch), vaccinated with one intraperitoneal injection of formalin-killed virulent aeromonas salmonicida cells suspended in saline, showed increased protection against approximately one ld60 of homologous challenge administered at 30 days post-vaccination. under similar conditions, coho vaccinated with a modified complete freund's adjuvant (mfca) alone were also equally protected. when measured against a more severe a. salmonicida challenge of approximately on ... | 1985 | 4043481 |
| methemoglobin reductase activity in fish erythrocytes. | nadh-methemoglobin reductase activity of erythrocytes from the coho salmon, oncorhynchus kisutch, sockeye salmon, oncorhynchus nerka, and the rainbow trout, salmo gairdneri exhibited a major band of activity that resembled the human enzyme in electrophoretic mobility. no polymorphism was found in 35 samples from rainbow trout, 4 samples from dolly varden, 29 samples from sockeye salmon, and 24 samples from coho salmon. all samples differed from the human enzyme in that they appeared to be membra ... | 1985 | 4085211 |
| characterization of anti-aeromonas salmonicida antibodies from coho salmon. | coho salmon (oncorhynchus kisutch) anti-aeromonas salmonicida agglutinins were excluded from sephadex g-200 and their activity was lost after partial reduction and alkylation followed by exposure to 2 m urea. immunoelectrophoretic analysis of coho antiserum revealed anodic and cathodic populations of immunoglobulin (ig), and the anti-a. salmonicida antibodies present 12 weeks after parenteral immunization were primarily anodic ig. antibodies were specifically purified by dissociation of a. salmo ... | 1974 | 4816458 |
| oral immunization of juvenile coho salmon against chondrococcus columnaris. bnwl-1050. | | 1970 | 5311306 |
| cortisol and its effects on plasma thyroid hormone and electrolyte concentrations in fresh water and during seawater acclimation in yearling coho salmon, oncorhynchus kisutch. | plasma cortisol, thyroxine (t4), and triiodothyronine (t3) concentrations increased during seawater (sw) acclimation in yearling coho salmon, oncorhynchus kisutch. maximal concentrations of cortisol (220 ng/ml) occurred within 1.5 hr after the ambient water was changed from fresh water (fw) to sw; after 21 days in sw, cortisol levels were still slightly elevated (23 ng/ml) compared to those in fw fish (8 ng/ml). plasma t4 concentrations peaked (14 ng/ml) at 12 and 72 hr after exposure to sw, and ... | 1984 | 6092209 |
| the effect of insulin on amino acid metabolism and glycogen content in isolated liver cells of juvenile coho salmon, oncorhynchus kisutch. | amino acid transport and [14c]leucine incorporation into liver proteins as well as the secretion of proteins into incubation medium were studied in liver cells isolated from coho salmon (oncorhynchus kisutch) parr. pink salmon (oncorhynchus gorbuscha) or mammalian (bovine) insulin caused a significant increase in tca-precipitable radioactivity from both cells and incubation medium. the effects appeared at insulin concentration of 10(-8) m with a maximal response at 5 x 10(-8) m. the radioactivit ... | 1984 | 6149052 |
| detection of infection and susceptibility of different pacific salmon stocks (oncorhynchus spp.) to the haemoflagellate cryptobia salmositica. | the haematocrit centrifugation technique, modified by keeping the haematocrit tubes cold (between 1 and 10 c), was sensitive for detecting light infections of cryptobia salmositica (as few as 75 flagellates per ml of blood). in wet mount preparations, infections lighter than 7.5 x 10(3) flagellates per ml of blood could not be detected consistently. different pacific salmon stocks from british columbia demonstrated differences in susceptibility to c. salmositica in experimental studies using lab ... | 1984 | 6470889 |
| toxicological response and its reversibility in rats fed lake ontario or pacific coho salmon for 13 weeks. | lake ontario coho salmon were known to contain a mixture of chemical contaminants. a previous study demonstrated that rats fed the lake ontario fish-supplemented diet for 28 days exhibited mild biochemical and histological changes. the purpose of the present study was to determine the effects due to a longer term of exposure and the reversibility of these effects. growth rate and food consumption were not affected by feeding the animals with lake ontario or pacific fish-supplemented diets for 13 ... | 1984 | 6520340 |
| development-associated changes in thyroxine kinetics in juvenile salmon. | smoltification is a transformation that occurs in some species of salmon, during which solitary fish in fresh water become schooling fish and migrate to the sea. this process is accompanied by large increases in plasma t4. t4 secretion rate and other parameters of t4 metabolism in juvenile coho salmon were estimated by applying kinetic analyses to measurements of the disappearance of injected t4 radiotracer from plasma. studies were performed at the beginning (march) and end (may) of the increas ... | 1984 | 6734520 |
| environmental salinity and the thyroidal response to thyrotropin in juvenile coho salmon (oncorhynchus kisutch). | the effect of environmental salinity on the thyroidal response, measured by plasma concentrations of thyroxine (t4), to exogenous bovine thyrotropin (btsh) was assessed early and late in the smoltification of coho salmon (oncorhynchus kisutch). a single injection of 100 miu btsh significantly increased plasma t4 concentrations in fish in both fresh water and seawater, and both early (march 19) and late (may 24) in smoltification. in fish transferred to seawater, the increase in t4 titers was som ... | 1984 | 6736902 |
| thyroid responses in rats fed diets formulated with great lakes coho salmon. | | 1982 | 7126925 |
| comparison of thyroid hormone binding to heptic nuclei of the rat and a teleost (oncorhynchus kisutch). | a modified t3 binding assay was used with coho salmon liver nuclei. purified nuclei were incubated with [125i]t3 (or [125i]t4), nonradioactive thyroid hormone, or thyroid hormone analog. bound hormone was separated from hormone by use of polyethylene glycol. salmon liver nuclei specifically bound t3 with a high affinity [ka = 1.031 +/- 0.174 x 10(9) m-1 (mean +/- se); n = 12] which was 50% of the affinity measured with rat liver nuclei using the same assay. salmon nuclear t3-binding capacity was ... | 1982 | 7140642 |
| toxicological response of rats fed lake ontario or pacific coho salmon for 28 days. | | 1981 | 7338593 |
| pyruvate kinases of salmon: purification and comparison with the isozymes from birds and mammals. | pyruvate kinase occurs as two major forms in coho salmon; the type m isozyme occurs primarily in muscle and heart, but type k has a more generalized tissue distribution, in parallel with the type k isozyme in other vertebrate systems. in order to assess the evolutionary relationships among the fish, avian, and mammalian isozymes of pyruvate kinase, we have purified the two isozymes from fish, have examined some of their physical properties, and have studied their immunological relationships to t ... | 1980 | 7373271 |
| effects of sodium dichromate exposure on the immune responses of juvenile coho salmon, oncorhynchus kisutch, against vibrio anguillarum. | sublethal exposure of juvenile coho salmon (oncorhynchus kisutch) to cr (as sodium dichromate) in freshwater decreased their disease resistance and serum a-glutinin production against vibrio anguillarum. duplicate disease resistance experiments, in which groups of salmon were exposed to 0.0 or 0.5 mg cr/l for two weeks and then injected in subgroups with one of four tenfold dilutions of live v. anguillarum (ca. 3-4 x 10(2-5) bacteria/.1 ml dose), showed that disease mortality increased with dose ... | 1980 | 7387188 |
| metabolism of coho salmon ig. catabolic rate of coho salmon tetrameric ig in serum. | | 1980 | 7393235 |
| structure of thyroid and adrenal glands in rats fed diets of great lakes coho salmon (oncorhynchus kisutch). | | 1980 | 7428764 |
| structure, function, and phylogeny of [arg8]vasotocin receptors from teleost fish and toad. | [arg8]vasotocin (avt) is considered to be the most primitive known vertebrate neurohypophyseal peptide of the vasopressin/oxytocin hormone family and may thus be ancestral to all the other vertebrate peptide hormones. the molecular evolution of the corresponding receptor family has now been studied by cloning an avt receptor, consisting of 435 amino acid residues, from the teleost fish, the white sucker catostomus commersoni. frog oocytes injected with the avt receptor-encoding crna respond to t ... | 1994 | 7509069 |
| insulin and igf-i binding and tyrosine kinase activity in fish heart. | a study is presented of the binding of insulin and igf-i to their respective receptors in the heart muscle of carp (cyprinus carpio), coho salmon (oncorhynchus kisutch), brown trout (salmo trutta fario) and sprague-dawley rats. receptor preparations were partially purified by wheat germ agglutinin affinity chromatography. specific binding of insulin/100 mg cardiac muscle was much lower in fish (from 5.6 to 9.2%) than in rat (52.0 +/- 5.0%). in both carp and trout, insulin binding to the cardiac ... | 1995 | 7561619 |
| increases in gill cytosolic corticosteroid receptor abundance and saltwater tolerance in juvenile coho salmon (oncorhynchus kisutch) treated with growth hormone and placental lactogen. | juvenile coho salmon (oncorhynchus kisutch) were injected with one of two recombinant bovine hormones, growth hormone (bgh; 5.0 and 0.5 micrograms.g-1 body wt) or placental lactogen (bpl; 5.0, 0.5, and 0.05 micrograms.g-1 body wt) to determine the effect on growth, plasma cortisol concentration, cytosolic corticosteroid receptors (cr) in the gills, and the development of hypoosmoregulatory ability. one week following a single injection or six weekly injections of bgh or bpl, the fish were measur ... | 1995 | 7781958 |
| abundant insulin-like growth factor-1 (igf-1) receptor binding in fish skeletal muscle. | insulin and insulin-like growth factor-1 (igf-1) receptors were quantified in glycoprotein fractions prepared by wheat germ agglutinin-agarose affinity chromatography from skeletal muscle of several species of salmonid fish and carp. insulin binding in fish skeletal muscle was lower than insulin binding found in rat. igf-1-specific binding was two- to sixfold higher than insulin binding (16.0 +/- 3.0 versus 5.8 +/- 0.75%/50 micrograms glycoprotein in brown trout, 15.5 +/- 0.9 versus 2.2 +/- 0.5% ... | 1995 | 7781961 |
| development of a homologous radioimmunoassay for coho salmon insulin-like growth factor-i. | a specific homologous radioimmunoassay (ria) for measurement of insulin-like growth factor-i (igf-i) in plasma of salmonid and a few non-salmonid fish species was developed using recombinant coho salmon igf-i (rsigf-i) as tracer and standard, and antiserum against this peptide raised in rabbits. the minimum detection level of igf-i was 1.5 ng/ml and linearity was obtained in a range from 1.5 to 23 ng/ml. no cross-reaction was detected in the salmon igf-i ria with mammalian growth factors, salmon ... | 1994 | 7843563 |