| [influence of loach egg- and embryo-extract on the activity of lactate dehydrogenase in the presence of insulin]. | studies of the pig muscle lactate dehydrogenase (ldh) activity were permormed after incubating the enzyme solution with extracts of loach (misgurnus fossilis) eggs and embryos, which were subjected to the influence of insulin hydrocortisone as well as to insulin combination with actinomycin d, cycloheximide or puromycin. the insulin alone is established to decrease the inactivating ability of the investigated extracts on the lactate dehydrogenase activity, when antibiotics removed to a considera ... | 1978 | 571156 |
| [the species specificity of fish and mammalian gonadotropic hormones]. | protein spectra of the hypophyses of fishes from various taxonomic groups obtained by disc electrophoresis in polyacrylamide gel exhibit high species specificity. by means of biological testing on the loach misgurnus fossilis, zones of gonadotropic activity were obtained which differ in various species by the electrophoretic mobility. the mammalian gonadotropins obtained reveal lower electrophoretic mobility. the gonadotropins investigated significantly differ in their biological effect. | 1976 | 941577 |
| [electrophysiologic parameters of ion transport systems in early developmental stages of fish and amphibia]. | it is shown that the membrane potential level, ionic current and membrane conductance depend on the cell cycle stage both in misgurnus fossilis l. embryos and in xenopus laevis daudin embryos by the microelectrode technics. allowing for the role of the adenylate cyclase system in the membrane potential oscillations and ion conductance of membranes, some series of experiments for analysis of the inhibitor have been carried out. | 1992 | 1340443 |
| [the detection and characteristics of substances with fibroblast growth factor properties in the cells of loach embryos]. | the substances extracted and partially purified from embryos of the loach (misgurnus fossilis l.) at the late blastula stage are shown to possess properties characteristic of the fibroblast growth factor (fgf): 1) they bind with heparin-sepharose; 2) induce dna synthesis in mouse nih-3t3 and swiss-3t3 fibroblast cell lines and in the primary culture of human embryonic fibroblasts; 3) have an apparent molecular weight of 15-16 kda (according to sds-electrophoresis); and 4) show positive reaction ... | 1992 | 1495756 |
| [detection of protease-sensitive factors with properties of beta-type transforming growth factor in early loach embryos]. | the blastoderm and yolk of misgurnus fossilis l. late blastulae were subjected to acid-ethanol extraction and gel filtration on biogel p10 in 1 m ch3cooh. this resulted in isolation of polypeptides that induce substrate-independent proliferation of nih-3t3 mouse fibroblasts in presence of epidermal growth factor. they did not compete for epidermal growth factor receptors of a-431 cells and inhibited substrate-independent proliferation of human lung carcinoma a-549 cells. these properties of the ... | 1990 | 2192328 |
| the 5'-flanking sequence of the loach oocyte 5s rrna gene contains a signal for effective transcription. | deletion mutants of loach oocyte 5s rrna genes were injected and transcribed in vivo in the nuclei of loach (misgurnus fossilis) and xenopus laevis. a control region was found in the 5'-flanking sequence, the elimination of which greatly decreases in vivo transcription of 5s rrna genes. this cis-acting element is located in the region between nt-18 and the transcription start point. we propose that the oocyte nucleus contains (a) specific transcriptional factor(s), ntfo, which interacts with the ... | 1990 | 2401403 |
| [integration and expression of the human somatotropic hormone gene in teleostei]. | plasmid dna containing human growth hormone gene was microinjected into cytoplasm of loach (misgurnus fossilis l.) fertilized eggs. after plasmid injection, more than 50% of embryos reached the hatching stage. in control experiments embryogenesis was completed giving 72.5% of injected and 90% of intact larvae. southern blot hybridization analysis revealed integration of injected recombinant dna constructions into fish chromosome's dna (2-30 copies per genome), without any significant rearrangeme ... | 1989 | 2471672 |
| [intragenomic polymorphism of the primary structure of 5s rrna gene variants of the loach (misgurnus fossilis l.). determination of the transcriptional activity]. | the primary structure of 12 cloned repeats of loach oocyte 5s rrna genes was determined. the heterogeneity of nucleotide sequences was revealed in the coding regions and spacer of the genes. the results of the study on in vivo transcriptional activity of the cloned 5s rrna gene variants are consistent with the localisation of site specific base substitutions in the coding part affecting the transcription. we have compared the nucleotide sequences of loach 5s rrna gene variants and of xenopus lae ... | 1989 | 2608037 |
| [content of d-looped molecules in mitochondrial dna of unfertilized oocytes and developing embryos of misgurnus fossilis]. | | 1974 | 4424513 |
| the influence of adaptation temperature on the behaviour of l-alanine:2-oxoglutarate aminotransferase of the skeletal muscle of pond loach (misgurnus fossilis l.). | | 1973 | 4727864 |
| [dna-methylase of loach (misgurnus fossilis) embryos]. | | 1971 | 4944920 |
| effect of microinjections of camp-dependent protein kinase subunits on macromolecular syntheses in loach misgurnus fossilis l. embryos. | the level of camp and the effects of the regulatory and catalytic subunits of camp-dependent protein kinase of type ii on protein, rna and dna synthesis in loach embryos were analysed. it was found that the injections of the catalytic subunit cause a sharp decrease in the rate of macromolecular synthesis while an increase in the concentration of this protein leads to the death of the embryo. injections of the regulatory subunit result in marked stimulation of protein, rna and dna synthesis. the ... | 1984 | 6089837 |
| [organization of genes coding for 5s rrna in the loach misgurnus fossilis l]. | the organization of 5s rrna genes in the loach misgurnus fossilis l. was studied. 5s rdna was cloned in escherichia coli using the hindiii-fragments of loach genome dna fused into the plasmid pbr322 restricted at the same site. the recombinant clones were tested by colony hybridization. the presence of the 5s rdna structural sequences in cloned fragments was determined by the southern procedure of hybridization with 5s [32p]rrna of the loach and transcription in the oocyte test system. it was fo ... | 1984 | 6095029 |
| [study of a cloned ribosomal operon region coding for 5.8s rrna in the loach misgurnus fossilis l]. | a fragment of the loach (misgurnus fossilis l.) ribosomal operon containing 5.8s rdna and adjacent regions of the internal transcribed spacer (its-1, and its-2) was sequenced. the 5'-terminal sequencing in 5.8s rdna was corrected by analysing the primary structure of the loach 5.8s rrna. this rna was shown to be presented by three types of molecules; one of these was shorter by 4 nucleotides at the 5'-end because of the processing site being shifted in the rrna precursor. the two other types dif ... | 1984 | 6095030 |
| [gamma-glutamyltransferase activity of different sections of the vertebrate brain]. | the activity of gamma-glutamyl transferase (ggtf; ec 2.3.2.2) has been measured in the cerebral hemispheres, cerebellum, hippocamp and brain stem of mature wild and laboratory rodents (oryctolagus cuniculus, cavia porcellus, rattus norvegicus, var. alb., ochotona pusilla, mesocricetus auratus, clethrionimus glareolis, mus musculus. var. alb.), as well as in homogenates of the whole brain of the tortoise testudo horsfieldi, frog rana temporaria and loach misgurnus fossilis. in wild rodents the ac ... | 1982 | 6126976 |
| ornithine decarboxylase activity in embryos depends on temperature of development rather than on the stage of development. molecular adaptation to temperature changes in poikilothermic animals. | the activity of ornithine decarboxylase (odc) (the key enzyme of polyamine synthesis) in different poikilothermic animals depends on the temperatures at which they were kept just before the enzyme assay. with an increase in temperature (within physiological limits) odc activity rises 5-25-fold within several hours. with a decrease in temperature it falls at the same rate. this effect, studied on loach (misgurnus fossilis) embryos in detail, was also shown for embryos, larvae and some adult tissu ... | 1983 | 6365078 |
| effect of spermine on interaction of dna polymerase alpha from the loach (misgurnus fossilis) eggs with dna. | polyamines (putrescine, spermidine and spermine) cause a marked increase in the activity of the loach misgurnus fossilis dna polymerase alpha on activated (gapped) dna. the stimulatory effect increases in the order: putrescine, spermidine, spermine. kinetic analysis shows that spermine does not change the affinity of the polymerase for dttp, but it decreases the enzyme affinity for dna. the apparent km of the polymerase for activated dna progressively increases from 14 to 1200 microm (nucleotide ... | 1984 | 6548155 |
| the primary structure of oocyte and somatic 5s rrnas from the loach misgurnus fossilis. | somatic and oocyte 5s rrnas from the liver and unfertilized eggs of the loach (misgurnus fossilis have been sequenced and found to differ in six nucleotides. all the substitutions are confined to the 5'-half of the molecules; 4 of them are pyrimidine-pyrimidine substitutions, and 2 are purine-pyrimidine ones. considerable differences, both in the position and the character of substitutions, have been established when these 5s rrnas were compared with somatic and oocyte 5s rrnas from xenopus bore ... | 1981 | 7197777 |
| [purification and properties of ribonuclease from the eggs and embryos of the loach misgurnus fossilis]. | endo- and exoribonucleases from loach eggs and embryos were partially purified. endoribonuclease from loach embryos has a ph optimum of 8.4, is thermostable, has a molecular weight of about 37 000, requires ca2+ for its activity, rapidly splits poly-u and slowly splits poly-c and does not hydrolyze poly-a. exoribonuclease has a ph optimum of 7.6 and is relatively thermostable; its activity is independent of ca2+, it has a molecular weight of 12 000, preferentially hydrolyzes rna of yeast and pol ... | 1981 | 7317535 |
| [analysis of expression of the rsv-lacz-gene in transgenic embryos of the loach misgurnus fossilis l. during different variations of injection]. | several series of microinjection of the rsv-lacz gene (the escherichia coli beta-galactosidase gene under the control of the long terminal repeat of the raus sarcoma virus) into fertilized mud loach eggs were carried out. the expression of the transgene in 3-5 day-old fry was shown to depend neither upon the stage of fry development at which the rsv-lacz gene was introduced (early blastodisc, late blastodisc, 2-blastomere embryo) nor upon the region of transgene injection (blastodisc cytoplasm o ... | 1995 | 7635315 |
| 5s rrna gene hybridizes to human chromosome 1 at two sites (1q42.11-->q42.13 and 1q43). | hybridization of a loach (misgurnus fossilis) oocyte 5s rrna gene repeat with human chromosomes was carried out to refine the localization of the 5s rna gene in the human genome. preliminary in situ hybridization analysis showed that this repeat hybridized with human chromosome region 1q42-->q43, the same location previously reported for the human 5s rrna gene. high-resolution banding revealed the presence of two sites of hybridization, with a main peak in chromosome region 1q42.1 and a second p ... | 1995 | 7956368 |
| a novel sialidase capable of cleaving 3-deoxy-d-glycero-d-galacto-2-nonulosonic acid (kdn). | we have examined the tissues of several species of fish and found that the liver of the loach (misgurnus fossilis) contains a novel sialidase capable of efficiently hydrolyzing 3-deoxy-d-glycero-d-galacto-2-nonulosonic acid (kdn) from the 4-methylumbelliferyl alpha-ketoside of kdn, kdn alpha 2-->3gal beta 1-->4glccer and kdn alpha 2-->6 n-acetylgalactosaminitol as well as neu5ac from the 4-methylumbelliferyl alpha-ketoside of neu5ac and gm3. the ph optimum for this enzyme was determined to be 4. ... | 1994 | 8161211 |
| transduction of ca2+ signals upon fertilization of eggs; identification of an s-100 protein as a major ca(2+)-binding protein. | a transient increase in the level of free cytosolic ca2+ is observed upon fertilization of the eggs of many species and is thought to represent a key event in the initiation of development. to identify components in the egg which could be involved in mediating such ca2+ signals we searched for ca(2+)-binding proteins in eggs of the fresh-water fish misgurnus fossilis (loach). we show that loach eggs contain two major ca(2+)-binding proteins which can be purified through their ca(2+)-dependent in ... | 1993 | 8217841 |
| [expression of the beta-galactosidase gene in transgenic embryos of the loach misgurnus fossilis l]. | in order to study the topological characteristics of transgene expression in individuals, early loach embryos were injected with recombinant plasmid fragment, containing lacz gene under the control of raus sarcoma virus ltr. the expression of rsv-lacz construct was found out in 80% of twenty-hours' embryos, 100% 2-3 days' embryos and 40% of 6-8 days' frys. point and dot expression was observes on the surface of the head, muscle segments, the yolk sac, in the yolk, on the caudal fin. the number, ... | 1993 | 8392961 |
| [expression of the cmv-lacz- and rsv-lacz-genes in transgenic fish and mouse embryos]. | the bacterial gene for beta-galactosidase under the control of ltr from either human cytomegalovirus (cmv-lacz) or the rous sarcoma virus (rsv-lacz) was injected into fertilized eggs of misgurnus fossilis l. loaches and f1 hybrid mice (cba x c57bl). expression of the cmv-lacz was observed in almost 100% of the loach embryos and larvae for two months following the first day of embryonic development. in some cases, expression points were located only on either the right or the left side of a fish. ... | 1996 | 9102356 |
| [a protein of the annexin group in early fish development. i. identification and biosynthesis of the protein in embryogenesis]. | ca-binding proteins were isolated from the loach (misgurnus fossilis) eggs and embryos, using their capacity for binding to acidic phospholipids in the presence of ca ions. it was shown that the major protein present in a mixture of these proteins was synthesized at the early developmental stages. polyclonal rabbit antibodies were raised against this protein. these antibodies proved to be monospecific both to the protein of the loach eggs and embryos and that of the brachydanio rerio embryos. th ... | 1998 | 9702792 |
| protein kinase c and casein kinase 2 phosphorylate in vitro proteins of the annexin family from eggs of loach misgurnus fossilis. | a mixture of proteins of the annexin family was obtained from the cytoplasm of mature eggs of loach misgurnus fossilis (by reprecipitation with acid phospholipids in the presence of ca2+). this mixture comprised five proteins with molecular weights of 58, 38, 36, 35, and 31 kd. polyclonal rabbit antibodies against the major 31-kd protein were obtained. western blot analysis showed that the obtained antibodies exhibit a high specificity towards the 31-kd protein from eggs and other tissues of loa ... | 1998 | 9795278 |
| exclusively juvenile centrioles in xenopus laevis oocytes injected with preparations of mature centrioles. | activated oocytes of xenopus laevis were injected with centriole preparations isolated either from spermatozoa of loach fish misgurnus fossilis or from rat liver. these injections induced the development of cytasters in the ooplasm and egg cleavage. electron microscopic study of cytasters was made at the stage that corresponded to interphase between first and second cleavage divisions. this study revealed in cytasters singleton centrioles surrounded by pericentriolar material and numerous microt ... | 1999 | 10211676 |
| preferential interaction of loach dna polymerase delta with dna duplexes containing single-stranded gaps. | we studied the interaction of dna polymerase delta (pol delta) purified from the eggs of the teleost fish misgurnus fossilis (loach) with dna duplexes containing single-stranded gaps of 1-13 nucleotides (nt). in the absence of processivity factors (pcna, rf-c, and atp), pol delta elongated primers on single-stranded dna templates in a distributive manner. however, the enzyme was capable of processive synthesis by filling gaps of 5-9 nt in dna duplexes. these data suggest that, upon filling a sma ... | 2000 | 11108834 |
| loaches in a long term study of the drava river in croatia. | the diversity, species composition and structure of the fish community were studied in the vicinity of three power plant reservoirs in the middle part of the drava river in croatia. over a period of twenty years in a collection of 25,913 fishes, 56 different fish species belonging to 16 families were recorded. the family cobitidae is represented by two species: cobitis elongatoides and misgurnus fossilis, while the family balitoridae is represented by only one species, barbatula barbatula. the p ... | 2003 | 15303354 |
| [the effect of regulatory sequences of alphas1-casein gene on the expression of the lacz-gene in loach misgurnus fossilis l. transgenic embryos]. | transient expression of recombinant plasmids carrying the lacz gene under the control of either bovine alphas1-casein gene tissue-specific promoter-enhancer region or highly homologous goat alphas1-casein gene promoter-enhancer region with supplementary regulatory sequences of the goat gene were studied in misgurnus fossilis l. loach embryos. it has been shown previously that the expression of the constructs carrying these regulatory elements in transgenic mice occurred primarily in the mammary ... | 2008 | 18767548 |
| transient expression and activity of human dna polymerase iota in loach embryos. | human dna polymerase iota (pol ι) is a y-family dna polymerase with unusual biochemical properties and not fully understood functions. pol ι preferentially incorporates dgtp opposite template thymine. this property can be used to monitor pol ι activity in the presence of other dna polymerases, e.g. in cell extracts of tissues and tumors. we have now confirmed the specificity and sensitivity of the method of pol ι activity detection in cell extracts using an animal model of loach misgurnus fossil ... | 2011 | 21983970 |
| redistribution of k+ in cleaving eggs of a fish misgurnus fossilis. | | 2014 | 4240046 |
| cdna cloning and expression characterization of serum transferrin gene from oriental weatherfish misgurnus anguillicaudatus. | in this study, the cdna coding serum transferrin (stf) of misgurnus anguillicaudatus (mastf) was cloned. mastf cdna is composed of 2326 bp with a 2007 bp open reading frame encoding 668 amino acids. the deduced mastf protein consists of a signal peptide, two lobes (n and c-lobes) and signature motifs of transferrin (tf) family. the results of tissue distribution indicated that mastf mrna was predominantly expressed in the liver. the results indicate that the mastf expression increased significan ... | 2014 | 24673686 |
| establishment and characterization of fin cell lines from diploid, triploid, and tetraploid oriental weatherfish (misgurnus anguillicaudatus). | continuous fin cell lines from diploid, triploid, and tetraploid oriental weatherfish, misgurnus anguillicaudatus, were established and characterized. the cell lines, designated dimf, trmf, and temf, respectively, were subcultured more than 80 times since initiation in october 2012 and were preserved at the china center for type culture collection as sample numbers c2013109, c2013110, c2013111, respectively. the cell lines consist predominantly of fibroblast-like cells. at the 50th passage, the ... | 2015 | 25721100 |
| the activity of some intracellular oxidative enzymes in the thyroid follicular epithelium of carassius auratus and misgurnus fossilis. | | 1977 | 18372 |
| study of rna transfer from nucleus to cytoplasm in early loach (misgurnus fossilis l.) and hybrid loach female times goldfish (carassius auratus auratus) male embryos. | | 1974 | 4428216 |
| [nuclear function during early development of remote fish hybrids]. | the participation of paternal genome was studied in the development of remote hybrids obtained as a result of artificial insemination of the loach (misgurnus fossilis) eggs by the sperm of aquarial cyprinids (brachydanio rerio, danio malabaricus, barbus tetrazona, razbora heteromorpha, carassius auratus) and cobitids (acanthophtalmus kuhlii). the hybrids obtained differed at the stage of hatching both from each other and from the loach by some morphological features. to study the function of het ... | 1974 | 4469618 |
| escape of rna to protein-synthesizing complexes of the cytoplasm in embryos of normal loach haploids and haploid hybrids between loach (misgurnus fossilis l.) and goldfish (carassius auratus auratus). | | 1973 | 4804061 |
| parvalbumin in skeletal muscles of teleost (tinca tinca l. and misgurnus fossilis l.). histochemical and immunohistochemical study. | musculatures of two fish species belonging to the suborder cyprinoidei were examined histochemically and immunohistochemically for demonstration of matp-ase activity and parvalbumin content, respectively. the tench lateral musculature shows a typical "acid reversal" picture of matp-ase activity--red fibres are alkali labile, acid stable and white and white fibres are alkali stable, acid labile. in the pond loach a superficial layer of musculature is composed of alkali--and acid stable red fibres ... | 1992 | 1483535 |
| weatherfish (misgurnus fossilis) as a new species for toxicity testing? | selection of appropriate test species is a critical issue when assessing effects of environmental contamination on fish because the ecological relevance of commonly used test species might be restricted due to their exotic origin. in the present study, a european freshwater fish with frequent occurrence in agricultural areas is suggested as a potential alternative: the european weatherfish (misgurnus fossilis). its suitability for acute embryo toxicity tests (fet) was investigated with regard to ... | 2017 | 27992775 |
| [comparative structural analysis of myosin light chains and gene duplication in fish]. | origin and structure of myosin light chain (mlc) proteins have been studied by comparative analysis of fish mlc1, mlc2, and mlc3 genes encoding mlc1, mlc2, and mlc3, respectively. the exon-intron structure of these genes has been analyzed in zebrafish danio rerio, loach misgurnus fossilis, fugu takifugu rubripes, and nile puffer tetraodon fahaka. we propose that mlc1 and mlc3 are homologues genes originated by fish-specific whole genome duplication (paralogs). this is supported by high sequence ... | 2016 | 16521537 |
| spatial-temporal characteristics of intercellular junctions in early zebrafish and loach embryos before and during gastrulation. | injections of lucifer yellow and fluorescein dyes into loach (misgurnus fossilis) and zebrafish (danio rerio) embryos were used to analyse the intercellular communication via gap junctions (gjs) and their role in morphogenetic processes during the period from early blastula to late gastrula. it is shown that the efficiency of dye transfer between the superficial blastomeres increases by the late blastula stage. blastomeres of the basal layer, on the other hand, become gradually uncoupled from th ... | 1997 | 27747404 |