| purification and properties of agglutinins from conger eel, conger myriaster (brevoort), skin mucus. | the skin mucus of the conger eel, conger myriaster, contains galactose-specific agglutinins. hemagglutinating activity is independent of divalent cations and is destroyed by heating at 50 degrees c for 15 min. the mucus agglutinins, named congerins, are a mixture of proteins with different electrical charges. three of these molecules were isolated by affinity chromatography on acid-treated sepharose 4b and by ion-exchange chromatography. they are simple proteins with the same molecular weight of ... | 1988 | 3384160 |
| conger myriaster, a new second intermediate host of heterophyopsis continua (digenea:heterophyidae). | six metacercariae were found from the gill filaments of conger myriaster purchased at mokpo-shi in korea on 7 september, 1996. based on the morphology of the excysted specimen, we identified them as metacercariae of heterophyopsis continua. c. myriaster is a new intermediate host of h. continua in the literature. | 1996 | 9017916 |
| galectin containing cells in the skin and mucosal tissues in japanese conger eel, conger myriaster: an immunohistochemical study. | congerin is a beta-galactoside binding lectin (galectin) purified from the skin mucus of the japanese conger, conger myriaster. to clarify its tissue distribution and productive cells, several tissue samples including skin, buccal cavity wall, tang, pharynx, gills, esophagus, stomach, intestine, liver, kidney, spleen and ovary of conger were stained immunohistochemically using polyclonal rabbit anti-congerin serum. in the epidermis, a number of club cells were strongly stained. because no agglut ... | 2001 | 11356222 |
| molecular diversity of skin mucus lectins in fish. | among lectins in the skin mucus of fish, primary structures of four different types of lectin have been determined. congerin from the conger eel conger myriaster and ajl-1 from the japanese eel anguilla japonica were identified as galectin, characterized by its specific binding to beta-galactoside. eel has additionally a unique lectin, ajl-2, which has a highly conserved sequence of c-type lectins but displays ca(2+)-independent activity. this is rational because the lectin exerts its function o ... | 2003 | 14662297 |
| effects of the piezo-tolerance of cultured deep-sea eel cells on survival rates, cell proliferation, and cytoskeletal structures. | we investigated the pressure tolerance of deep-sea eel (simenchelys parasiticus; habitat depth, 366-2,630 m) cells, conger eel (conger myriaster) cells, and mouse 3t3-l1 cells. although there were no living mouse 3t3-l1 and conger eel cells after 130 mpa (0.1 mpa = 1 bar) hydrostatic pressurization for 20 min, all deep-sea eel cells remained alive after being subjected to pressures up to 150 mpa for 20 min. pressurization at 40 mpa for 20 min induced disruption of actin and tubulin filaments wit ... | 2005 | 16082498 |
| yeast-binding c-type lectin with opsonic activity from conger eel (conger myriaster) skin mucus. | a mannose-specific lectin with agglutination activity against a yeast, saccharomyces serevisiae, was purified from conger eel (conger myriaster) skin mucus. the lectin, named concl-s, has a tetrameric structure consisting of two non-covalently associated dimers whose constituent monomers with a molecular mass of about 16,000 da are linked by a disulphide bond. concl-s is composed of 173 amino acid residues including a 24-residue signal peptide, and belongs to the c-type lectin family, which is t ... | 2007 | 16753218 |
| transactivation activity of thyroid hormone receptors in fish (conger myriaster) in response to thyroid hormones. | we previously isolated cdnas encoding conger eel (conger myriaster) thyroid hormone (th) receptors (trs). in the present study, we investigated the transactivation activities of conger eel trs treated with ths (3,3',5-triiodo-l-thyronine [t3], l-thyroxine [t4], and 3,3',5'-triiodo-l-thyronine [rt3]), or ligands and activators of other nuclear receptors. following transient transfection into the japanese eel (anguilla japonica) hepatocyte cell line hepa-e1, the conger eel trs (ctrs) showed th-dep ... | 2006 | 16820313 |
| simultaneous detection and prevalence of allergens in anisakis species isolated from marine fishes. | this study was conducted to develop a multiplex reverse transcription (rt) pcr for the detection of anisakis allergens and to investigate the relationship between allergen profiles and anisakid larvae isolated from scomber japonicus, trichiurus lepturus, and conger myriaster in korea. the species of anisakis was determined using anisakis pegreffii-specific pcr and restriction fragment length polymorphism analysis. the prevalence and profiles of five ani s allergens were examined by multiplex rtp ... | 2016 | 27296426 |
| inactivation of anisakis simplex l3 in the flesh of white spotted conger (conger myriaster) by high hydrostatic pressure and its effect on quality. | koreans consume much seafood; the country is surrounded on the east, west and south by the sea. koreans have eaten raw sashimi for a long time. however, a concern in the raw sea food industry is that the parasitic nematode anisakis simplex l3 occurs naturally in marine fish. thus, the fishery industry needs a non-thermal processing method. high hydrostatic pressure (hpp) has been demonstrated to be effective. white spotted conger flesh containing 20 live larvae was exposed to different pressures ... | 2016 | 27117731 |
| differences and variations among anguilla japonica, muraenesox cinereus and conger myriaster from the yellow sea. | genomic dnas were extracted from the muscle of twenty-one specimens of three eel species collected in anguilla japonica (aj), muraenesox cinereus (mc) and conger myriaster (cm) from the yellow sea, respectively. in the present study, 7 oligonucleotides primers generated 191 specific loci in the aj species, 226 in the (mc) species and 181 in the cm species, respectively. the primer bion-02 generated the most loci (a total of 83), with an average of 11.86 in the aj species. the specific loci gener ... | 2015 | 27004273 |
| characterization of thyroid hormone receptors during early development of the japanese eel (anguilla japonica). | we studied the profiles of thyroid hormone receptors (trs) in japanese eels (anguilla japonica) during development from hatched larvae to juveniles. two trαs (trαa and trαb) and one trβ (trβa) cdna clones were generated by race. the trαa, trαb and trβa cdnas encoded 416, 407 and 397 amino acid proteins with much higher homologies to the japanese conger eel (conger myriaster) trs than to other fish trs. in a transiently transfected japanese eel cell line, hepa-e1, the trs showed thyroid hormone ( ... | 2013 | 24100168 |
| differences in organochlorine accumulation accompanying life history in the catadromous eel anguilla japonica and the marine eel conger myriaster. | in order to examine the ecological risk for organic pollutants in diadromous fish migrating between sea and freshwater, organochlorine compounds (ocs) were determined in the catadromous eel anguilla japonica having marine, estuarine and freshwater residence life histories. the eels were collected in japanese coastal areas. we also compared the ocs accumulation with the marine eel conger myriaster, which has a similar life history as a. japonica in the marine environment. the ontogenic changes in ... | 2012 | 22407403 |
| offshore spawning of conger myriaster in the western north pacific: evidence for convergent migration strategies of anguilliform eels in the atlantic and pacific. | the spawning area of the common japanese conger, conger myriaster, had remained unknown because spawning adults or its newly hatched larvae were never collected. using genetic identification, we determined that c. myriaster spawns far offshore in the western north pacific, just west of the spawning area of the japanese eel, anguilla japonica. in june 2008, six newly hatched c. myriaster larvae, 5.6-6.9 mm, were collected at the eastern edge of where many small unidentified conger leptocephali (7 ... | 2011 | 21509576 |
| thyroid hormone receptor beta is widely expressed in the brain and pituitary of the japanese eel, anguilla japonica. | in a previous study, we cloned the thyroid hormone receptor betab (trbetab) from a teleostean fish, the japanese conger eel (conger myriaster). the gene encoding this receptor is expressed in the brain and pituitary. in this study, we cloned trbetab from the brain of the japanese eel (anguilla japonica) to extend our studies into thyroid hormone function in fish. rt-pcr analysis demonstrated that the trbetab transcripts were abundant in both the brain and pituitary. the trbetab cdna encoded a 37 ... | 2007 | 17150218 |
| cloning and characterization of vitellogenin cdna from the common japanese conger (conger myriaster) and vitellogenin gene expression during ovarian development. | the major yolk protein precursor, vitellogenin (vtg) was detected in plasma from vitellogenic females and estradiol-17beta (e2)-treated immature females, but not in males and immature females by western blotting in common japanese conger conger myriaster. its molecular mass was approximately 180 kda under denaturing and reducing conditions. the common japanese conger vtg cdna was cloned from the liver of vitellogenic female. it contains 5110 nucleotides including an open reading frame that encod ... | 2006 | 16460978 |
| neoascarophis sinensis n. sp. (nematoda: cystidicolidae), a parasite of conger myriaster (brevoort) (anguilliformes: congridae) in chinese waters. | neoascarophis sinensis n. sp. collected from the whitespotted conger conger myriaster (brevoort) (anguilliformes: congridae) in the yellow and east china seas, is described using both light and scanning electron microscopy. the new species is characterised mainly by the body size (8.5-10.5 mm in the males, 9.5-14.0 mm in the females), the location of the vulva (near equatorial region of the body), the non-bifurcate deirids, the lengths of the vestibule (40-50 µm in the males, 30-60 µm in the fem ... | 2017 | 28324235 |
| immunoreactive endothelin in plasma of nonmammalian vertebrates. | immunoreactive endothelin (ir-et) was estimated in plasma of nonmammalian vertebrates, using radioimmunoassay (ria) for endothelin-1 (et-1). blood samples were collected from unanesthetized animals. plasma et was determined by ria after extraction. plasma levels of ir-et were 0.7 +/- 0.2 pg/ml (n = 6) in the hagfish eptatretus burgeri, 4.3 +/- 0.9 pg/ml (n = 5) in the banded dogfish triakis scyllia, 3.6 +/- 0.8 pg/ml (n = 3) in the common japanese conger conger myriaster, 9.6 +/- 1.4 pg/ml (n = ... | 1991 | 1725396 |
| anisakiasis: report of 15 gastric cases caused by anisakis type i larvae and a brief review of korean anisakiasis cases. | the present study was performed to report 15 anisakiasis cases in korea and to review the korean cases reported in the literature. total 32 anisakis type i larvae were detected in the stomach of 15 patients by the endoscopy. single worm was detected from 12 cases, and even 9 larvae were found from 2 cases. epigastric pain was most commonly manifested in almost all cases, and hemoptysis and hematemesis were seen in 1 case each. symptom manifestations began at 10-12 hr after eating fish in 73.3% c ... | 2015 | 26323845 |
| galectins in the abdominal cavity of the conger eel conger myriaster participate in the cellular encapsulation of parasitic nematodes by host cells. | congerin is a proto-type galectin distributed on the skin and mucosal epithelia of the upper digestive tract of the japanese conger eel conger myriaster. it has at least 2 isotypes, namely, congerin i and ii, and plays a role in bio-defense at the body surface. in the current study, we identified both isotypes in the peritoneal fluid and peritoneal cells of c. myriaster by western blot and mass spectrometry (ms)/ms analysis. cucullanus nematodes parasitize the abdominal cavity of c. myriaster, a ... | 2012 | 22820379 |
| metabolism of a glycosaminoglycan during metamorphosis in the japanese conger eel, conger myriaster. | hyaluronan (ha) is a linear polysaccharide of high molecular weight that exists as a component of the extracellular matrix. the larvae (leptocephali) of the japanese conger eel (anguilliformes: conger myriaster) have high levels of hyaluronan (ha) which is thought to help control body water content. we isolated glycosaminoglycans (gags) from japanese conger eel leptocephali and measured the changes in tissue ha content during metamorphosis. ha content decreased during metamorphosis. in contrast, ... | 2009 | 22820749 |
| isolation of epidermal cells and cdna cloning of tnf decoy receptor 3 of conger eel, conger myriaster. | by using edta and a trypsin solution, we established a method for isolating the epidermal cells of the conger eel, conger myriaster. we then identified tnf decoy receptor (dcr) cdna in the species from a suppression subtractive hybridization library prepared from the epidermal cells stimulated with lps. the full-length cdna of conger tnf dcr (condcr) consisted of 1479 base pairs, and the protein comprised 286 amino acid residues. phylogenetic analysis indicated that condcr was clustered into a d ... | 2008 | 18262436 |
| lps induces gene expression of interleukin-1beta in conger eel (conger myriaster) macrophages: first cytokine sequence within anguilliformes. | our aim was to comprehensively analyze the immune response in anguilliformes macrophages and to survey cytokine genes expressed from them. we therefore used suppression subtractive hybridization (ssh) to randomly clone molecules that are specifically expressed in conger eel (conger myriaster) macrophages when cells are stimulated by lps. as a result, we succeeded in identifying a conger eel il-1beta. this is the first report on cytokines in anguilliformes, which is the most ancient order in livi ... | 2007 | 17587597 |
| opsonic effect of congerin, a mucosal galectin of the japanese conger, conger myriaster (brevoort). | | 2006 | 16102974 |
| the speciation of conger eel galectins by rapid adaptive evolution. | many cases of accelerated evolution driven by positive darwinian selection are identified in the genes of venomous and reproductive proteins. this evolutional phenomenon might have important consequences in their gene-products' functions, such as multiple specific toxins for quick immobilization of the prey and the establishment of barriers to fertilization that might lead to speciation, and in the molecular evolution of novel genes. recently, we analyzed the molecular evolution of two galectins ... | 2004 | 14758068 |
| characterization of thyroid hormone receptor alpha and beta in the metamorphosing japanese conger eel, conger myriaster. | two distinct tr alpha cdna clones (tr alpha a and tr alpha b) were isolated from conger eel (conger myriaster). the deduced amino acid sequences of the conger eel tr alphas showed higher homologies to the tr alphas of other vertebrates than to tr betas. determination of tr mrna in metamorphosing eels was performed using competitive rt-pcr. of the two tr alpha mrnas identified, tr alpha a mrna expression was shown to be relatively higher than that of tr alpha b, and there was a peak in the expres ... | 2003 | 12812781 |
| cdna cloning of thyroid hormone receptor betas from the conger eel, conger myriaster. | two distinct trbetas cdna clones were generated by race from a conger eel (conger myriaster). the deduced amino acid sequences of the conger eel trbetas (ctrbetas) showed higher homologies to the known trbetas of other vertebrate animals than to tralphas. two ctrbetas possessed an insertion sequence in the hinge region similar to other teleost fish trbetas. variation in ctrbeta due to differential splicing in the hinge region of the ctrbetas genes was found using pcr analysis. the determination ... | 2003 | 12714004 |
| complete mitochondrial dna sequence of conger myriaster (teleostei: anguilliformes): novel gene order for vertebrate mitochondrial genomes and the phylogenetic implications for anguilliform families. | the complete nucleotide sequence of the mitochondrial genome was determined for a conger eel, conger myriaster (elopomorpha: anguilliformes), using a pcr-based approach that employs a long pcr technique and many fish-versatile primers. although the genome [18,705 base pairs (bp)] contained the same set of 37 mitochondrial genes [two ribosomal rna (rrna), 22 transfer rna (trna), and 13 protein-coding genes] as found in other vertebrates, the gene order differed from that recorded for any other ve ... | 2001 | 11343127 |
| accelerated evolution in the protein-coding region of galectin cdnas, congerin i and congerin ii, from skin mucus of conger eel (conger myriaster). | two cdnas encoding galectins named congerins i and ii from the skin mucus of conger eel (conger myriaster) were isolated and sequenced. comparison of the nucleotide sequences of congerins i and ii showed that the sequence similarities of the 5' and 3' untranslated regions (86 and 88%, respectively) were much higher than those of the protein-coding region (73%). the numbers of nucleotide substitutions per site (kn) for the untranslated regions are smaller than the numbers of nucleotide substituti ... | 1999 | 10478448 |
| functional and structural characterization of multiple galectins from the skin mucus of conger eel, conger myriaster. | the complete amino acid sequence of an isogalectin, named congerin ii, isolated from the skin mucus of conger eel, was determined by sequencing of the protein and its peptides generated by enzymatic and chemical cleavages. congerin ii consisted of 135 amino acids residues containing an acetylated n-terminus. congerin ii was found to be only 46% homologous in sequence to congerin i which was previously determined (muramoto k., kamiya h., biochem. biophys. acta, 1992;1116:129-136), suggesting that ... | 1999 | 10425711 |
| architecture of the lateral line organ of the sea eel conger myriaster. | the architecture of trunk canal neuromasts of the japanese sea eel was morphologically examined. a fluid-filled canal is connected with the outside by horn-shaped tubules whose outer openings are seen as pores, which are variable in shape and very narrow as compared to the canal diameter. a projection shaped like a semilunar valve hangs over each neuromast from the opposite wall. the total number of hair cells in a canal neuromast was 500 to 600, about fivefold more numerous than that in a super ... | 1995 | 8559560 |
| the amino-acid sequence of a lectin from conger eel, conger myriaster, skin mucus. | the amino-acid sequence of a beta-galactoside-binding lectin isolated from the skin mucus of the conger eel conger myriaster was determined. the lectin (30 kda) was composed of two identical subunits of 135 amino acid residues with n-acetylserine at the n-terminus and no half-cystinyl residue. it was a 30-34% sequence identical to vertebrate beta-galactoside-binding lectin and proved to be a member of the s-type lectin family. | 1992 | 1581341 |
| purification and characterization of a galactose-binding lectin from the skin mucus of the conger eel conger myriaster. | 1. a galactose-binding lectin was purified from the skin mucus of the conger eel conger myriaster by affinity chromatography and hplc. 2. the lectin was a simple protein having the same two subunits with a mol. wt of 12,500 and a n-terminal amino acid of phenylalanine. 3. electrofocusing suggested that the purified lectin was composed of several isolectins. 4. from the ultraviolet difference spectra attributable to tryptophanyl residues in the binding site, the binding constant of the lectin for ... | 1989 | 2924535 |
| presence of the ruffed cell in the olfactory bulb of the catfish, parasilurus asotus, and the sea eel, conger myriaster. | the ruffed cell is present in the olfactory bulb of the catfish, parasilurus asotus, and of the sea eel, conger myriaster. its morphological features have been studied by light microscopy, high-voltage electron microscopy, and conventional electron microscopy. the ruffed cell of the catfish is very similar to that of the goldfish in its location and in its structural features. it has a spheroidal or ovoid cell body about 15 to 30 micrometer in diameter. several dendrites arise from the cell body ... | 1980 | 7430425 |
| a study of the fine structure of the pit organ of the common japanese sea eel conger myriaster. | the fine structure of the pit organ of the sea eel has been studied by means of electron microscopy. the sensory epithelium of the pit organ consists of sensory cells and supporting cells. the apical surface of the sensory cell is studded with sensory hairs consisting of a kinocilium and stereocilia. the sensory cells are divided into two groups. in one, the kinocilium points dorsally and in the other the kinocilium points ventrally. the total number of sensory cells in one pit organ is about 10 ... | 1978 | 657251 |
| sterc-bile acids and bile sterols. 73. studies on the bile of conger myriaster. | | 1965 | 5891725 |