regeneration of lateral line and inner ear vestibular cells.labelling experiments with [3h]thymidine demonstrate a continuous production of cells in the mechanoreceptive lateral line organs of the eel (anguilla anguilla) and butterfly fish (pantodon buchholzi) as well as in the electroreceptive ampullary organ of the transparent catfish (kryptopterus bicirrhus). shortly after [3h]thymidine injection many cells are labelled in the middle and basal parts of the sensory organ and after a few days' survival sensory cells are also labelled. the vestibular sen ...19911752161
characterization of pore-forming activity in liver mitochondria from anguilla anguilla. two porins in mitochondria?a fast purification procedure for the isolation and purification of eukaryotic porin (de pinto et al., (1987) biochim. biophys. acta 905, 499-502) was applied to liver mitochondria of the fish anguilla anguilla. a protein preparation was obtained which formed slightly anionically selective pores in reconstitution experiments with lipid bilayer membranes. the distribution of single-channel conductances had two maxima of 2.4 ns and 4.0 ns in 1 m kcl. sodium dodecylsulfate electrophoretograms of th ...19911705440
lectins from triticum vulgaris and limax flavus are universal antagonists of botulinum neurotoxin and tetanus toxin.lectins from anguilla anguilla, artocarpus integrifolia, canavalia ensiformis, datora stramonium, glycine max, limax flavus, ricinus communis and triticum vulgaris were tested for their abilities to antagonize the binding of botulinum neurotoxin and tetanus toxin to rat brain membranes and to antagonize the ability of these toxins to block neuromuscular transmission in mouse phrenic nerve-hemidiaphragm preparations. lectins from limax flavus and triticum vulgaris, both of which have affinity for ...19911653841
distribution and pharmacological properties of the gabaa/benzodiazepine/chloride ionophore receptor complex in the brain of the fish anguilla the present study, we characterized the distribution and the pharmacological properties of the different components of the gabaa receptor complex in the brain of the eel (anguilla anguilla). benzodiazepine recognition sites labeled "in vitro" with [3h]flunitrazepam ([3h]fnt) were present in highest concentration in the optic lobe and in lowest concentration in the medulla oblongata and spinal cord. a similar distribution was observed in the density of gamma-[3h]aminobutyric acid ([3h]gaba) bi ...19892538558
chromatographic and immunological evidence for mammalian gnrh and chicken gnrh ii in eel (anguilla anguilla) brain and pituitary.gonadotropin-releasing hormone (gnrh) peptides in the brain and pituitary of the european eel (anguilla anguilla) were investigated by reverse phase high performance liquid chromatography (hplc) and radioimmunoassay with region-specific antisera. two gnrh molecular forms were demonstrated in brain and pituitary extracts. one form eluted in the same position as synthetic mammalian gnrh on hplc and was recognized by antibodies directed against the nh2 and cooh termini of mammalian gnrh as well as ...19902199948
[purification and characterization of presumed thyrotropic hormone subunits of a teleost fish, the eel (anguilla anguilla)].we have only a partial knowledge of fish thyrotropins (tsh) and no data about peptide sequence are available. various hplc techniques allowed us to purify a 31.4 kda, heterodimeric protein from saline eel pituitary extracts containing tsh activity. partial n-terminal sequence (24 amino acids) from one subunit was strictly identical to that of eel gonadotropin ii (gth ii) alpha subunit. with regard to the second subunit, 41% of the 22 amino acids identified at the n-terminus were homologous to th ...19911933511
in vivo and in vitro toxicity of fractionated fish lipids, with particular regard to their content of chlorinated organic compounds.six different lipid matrices (the intact lipid (il), four lipid fractions with different polarity, and the free fatty acids (ffas) obtained by hydrolysis of the triacylglycerol (tag) containing fraction) were obtained from salmon (salmo salar) and eel (anguilla anguilla), each collected at a contaminated and a comparatively uncontaminated catch site along the coast of scandinavia. the lipid matrices were studied in toxicological test systems representing various biological functions of different ...19911766922
occurrence of acanthocephalus clavula (acanthocephala) in echinogammarus pungens (amphipoda) from the river adige.cystacanths of acanthocephalus clavula dujardin 1845 (acanthocephala) were found in the hemocoels of naturally infected echinogrammarus pungens m. edwards 1840 (amphipoda). crustaceans were collected in porto frossone, an estuarine locality of the river adige. only 3 out of 562 e. pungens specimens were infected by a. clavula cystacanths; the larvae were enclosed in a thin acellular envelope. infected amphipods were all females, and their total length was greater than that of uninfected females. ...19911844501
potential interactions between acanthocephalus anguillae and pomphorhynchus laevis in their natural hosts chub, leuciscus cephalus and the european eel, anguilla anguilla.chub and eels were experimentally infected via intermediate hosts harbouring cystacanths, with pomphorhynchus laevis alone, or acanthocephalus anguillae alone, or simultaneously with mixtures of both species in varying proportions, and sampled at 7, 56 or 112 days post-infection. examination of chub revealed that both species showed low establishment and growth rates, differing markedly from british field data, where chub is apparently one of the most important hosts, and preventing further mean ...19911852495
lethal toxicity of lindane on a teleost fish, anguilla anguilla from albufera lake (spain): hardness and temperature effects.this paper reports the results of toxicity tests conducted using anguilla anguilla under three different water temperature (15, 22 and 29 degrees c) and two hardness regimes (250 and greater than 600 ppm caco3). the 96-h lc50 increased in the experimental medium (p less than 0.05) by an order of magnitude from 0.32 to 0.45 mg/l between 15 and 29 degrees c. however in the natural medium it is similar (p greater than 0.05) (0.54 to 0.55 mg/l) for these same temperatures. the toxicity of lindane on ...19882453551
effects of vertebrate prolactins and growth hormones on thyroxine 5'-monodeiodination in the eel (anguilla anguilla): a potential bioassay for growth hormone.growth hormones (ghs) and prolactins (prls) purified from representatives of each vertebrate class from bony fish onwards were tested for their ability to stimulate in vivo peripheral deiodination of labeled thyroxine (t4*) into triiodo-l-thyronine (t3*) in the eel. plasma t3*/t4* ratio was used as parameter. all ghs significantly increased t3*/t4*, the magnitude of the response being unrelated to the phylogenic position of species. no significant stimulation was shown with the various prl, with ...19892707580
triiodothyronine binding to putative solubilized nuclear thyroid hormone receptor in liver and gill of the brown trout (salmo trutta) and the european eel (anguilla anguilla).the binding of 3,5,3'-triiodothyronine (t3) to salt-extracted nuclear protein of liver and gill from trout and eel was studied in vitro. [125i]t3 binding depended on the temperature and protein concentration. binding equilibria were achieved in the two tissues of each species between 15 and 24 hr at 4 degrees. the binding was reversible in the presence of excess unlabeled t3. scatchard analysis showed a single class of high affinity and low capacity t3 binding species considered, 2.71 x 10(-10) ...19892806877
estradiol has inverse effects on pituitary glycoprotein hormone alpha-subunit messenger ribonucleic acid in the immature european eel and the gonadectomized teleosts, the pituitary contains a single glycoprotein gonadotropic hormone (gth) composed of two dissimilar alpha- and beta-subunits. the european eel, anguilla anguilla l, is sexually immature at the silver stage due to a deficiency in gth synthesis and secretion. in previous studies we (s.d., ya.f.) have demonstrated a strong stimulatory action of estradiol (e2) on eel pituitary gth content. in contrast, we (r.c., m.j.) have shown that in the rat e2 negatively regulates gonadotropin subuni ...19872441980
saccharide residues in human gingiva as revealed with fluorochrome-coupled lectins.the histochemical binding of 16 fluorochrome-conjugated lectins to human marginal gingiva was investigated. of a total of 14 galactose/n-acetylgalactosamine (gal/galnac)-specific lectins, dolichos biflorus (dba), helix pomatia (hpa), and helix aspersa agglutinins (haa) were blood group a-reactive whereas griffonia simplicifolia i-b4 (gsa-i-b4) and sophora japonica (sja) agglutinins were blood group b-reactive. hpa, haa and gsa-i-b4 bound to all suprabasal epithelial cells and to vascular endothe ...19892542513
difference in the ability of blood group-specific lectins and monoclonal antibodies to recognize the abh antigens in human tissues.twelve different kinds of blood group-specific lectins have been used along with monoclonal anti-a, -b and -h antibodies for detecting the corresponding antigens in selected human tissues. although most of the lectins recognized the antigens in the tissue sections examined, they displayed marked differences in their recognition patterns in certain tissues. helix asparsa agglutinin (haa), helix pomatia agglutinin (hpa) and monoclonal anti-a antibody recognized a antigens in the mucous cells of sa ...19901705925
effects of lindane on fish carbohydrate metabolism.exposure of the european eel (anguilla anguilla) to a high sublethal concentration of 0.335 ppm (0.50 of the 96-hr lc50) of lindane for 6, 12, 24, 48, 72, and 96 hr affected carbohydrate metabolism. muscle glycogen levels decreased significantly (p less than 0.05) at 6, 12, 24, 48, 72, and 96 hr; liver glycogen content did not decline at any time. muscle glucose levels in fish were elevated at 6, 12, 24, 48, 72, and 96 hr but in liver, the levels increased only at 96 hr. mean values of muscle an ...19911717227
cloning and sequence analysis of the cdna for the pituitary glycoprotein hormone alpha-subunit of the european eel.a cdna library constructed using mrnas isolated from pituitary glands of estradiol-treated eels was screened with a cdna fragment for the rat glycoprotein hormone alpha-subunit. three out of 10,000 cdna clones were revealed and subcloned in puc13 for characterization and sequencing. all three had the same nucleotide sequence except for a single, silent change in the coding sequence for one of them, and for the location of the poly(a) tail. analysis of the deduced amino acid sequence strongly sug ...19901698669
[implication of growth hormone in experimental induction of vitellogenesis by estradiol-17 beta in female silver eel (anguilla anguilla l.)].the effect of different preparations of growth hormone (gh) was assayed with 17 beta-estradiol on vitellogenesis in hypophysectomized or normal female silver eels. vitellogenin (vg) plasma levels were taken as the index of hepatic vitellogenesis. the e2 doses were chosen to give the same pattern for the plasma vg level as in the controls. they decreased or remained undetectable in hypophysectomized or normal animals. gh also failed to induce alone a significant modification. when e2 was injected ...19921638433
sensitivity of eels (anguilla anguilla) and carp (cyprinus carpio) to type c and e botulinum toxin.this study has shown for the first time that fish such as eels (anguilla anguilla l.) and carp (cyprinus carpio l.) are sensitive to type e botulinum toxin. with oral administration the mld for eels was 27.500 mouse ld50 per kg body weight, while for carp it was only 236 mouse ld50 per kg. carp were also found to be sensitive to a slight degree to type c botulinum toxin, the mld of the most virulent toxin of this type being 457,000 mouse ld50 per kg with oral administration. eels, however, showe ...19751179868
a comparison of highly unsaturated fatty acid levels in wild and farmed eels (anguilla anguilla).1. absolute and relative amounts of eicosapentaneoic acid (epa) and docohexaenoic acid (dha) in muscle of eels from four different fish farms were compared with samples from wild eels from two different areas of northern italy. 2. farmed eels were richer in dha and epa than wild animals. 3. the addition of cod liver oil to the diet of farmed eels led to a significant accumulation of epa and dha, but no change in total lipid content. 4. the calculation of two indices related to highly unsaturated ...19921499279
cutaneous respiration in seven sea-water teleosts.the oxygen consumption of excised skin (mo2 is.cut.) and the normal cutaneous oxygen uptake by the skin in situ from the external medium (mo2 ext.cut.) were investigated in seven species of teleosts in normoxic sea water: butterfish (pholis gunnellus l.), cod (gadus morhua l.), five-bearded rockling (ciliata mustela l), shanny (blennius pholis l.), flounder (platichthys flesus l.), sole (solea solea l.) and eel (anguilla anguilla l.). in the butterfish, cod and rockling, all the oxygen absorbed ...1978741099
effects of desoxycorticosterone acetate on renal structure in the mouse and in the european eel.the administration of desoxycorticosterone acetate (doca) induces morphological changes in the kidney of mice and eels. such changes mainly concern the cells of the distal tubules and collecting tubules. these changes are consistent with the hypothesis that the hormone would exert a different action in mammals and in teleosts.1978747222
affinity purification of antigen-specific serum immunoglobulin from the european eel (anguilla anguilla).immunization of specimens of the european eel with a hapten-carrier conjugate resulted in a significant rise in anti-hapten titres. antigen-specific immunoglobulin was purified on a matrix onto which the hapten-carrier conjugate had been immobilized. rabbit antisera raised against the product of the affinity purification recognized two molecular moieties. the first was eel immunoglobulin as inferred by the polypeptide composition, i.e. disulphide-linked heavy and light chains of 72 kda and 25 kd ...19921615286
[calcitonin in the ultimobrancial body of anguilla (anguilla anguilla l.): cytologic localization by indirect immunofluorescence using human anti-salmon-calcitonin antibodies].the localization of intracellular calcitonin has been achieved by immunofluorescence in the cytoplasm of all cells forming the epithelium of the ultimobranchial body of eels, using a human antiserum against synthetic salmon calcitonin i. the specificity of the reaction is demonstrated by inhibition with synthetic salmon calcitonin (s.c.t.); the fluorescence is not inhibited by synthetic human calcitonin (h.c.t.).1977409547
studies on lectins. xxxvi. properties of some lectins prepared by affinity chromatography on o-glycosyl polyacrylamide gels.a number of lectins has been purified by affinity chromatography on o-glycosyl polyacrylamide gels. the lectins isolated (and the particular sugar ligands used in the affinity carriers) are as follows: anguilla anguilla, serum (alpha-l-fucosyl-), vicia cracca, seeds; phaseolus lunatus, seeds; glycine soja, seeds; dolichos biflorus, seeds; maclura pomifera, seeds; sarothamnus scoparius, seeds; helix pomatia, ablumin glands; clitocybe nebularis, fruiting bodies (all n-acetyl-alpha-d-galactosaminyl ...1978563738
[chromatographic separation and determination of arginine-vasotocin and isotocin in the pituitary and preoptic nucleus of the north sea eel (anguilla anguilla l)]. 19705485276
[studies on the morphology of blood of the european eel (anguilla agnuilla). iii. studies on monocytic and lymphoid cells].investigations concerning the monocytic and lymphocytic cell series were carried out on the peripheral blood and dabbings of the kidney, the spleen and intestines of healthy eels (anguilla anguilla), and eels taken ill with vibrio anguillarum. by means of histological, cytochemical, phaseoptical, and electronmicroscopical examinations independent development lines of the monocytes and lymphocytes were established. the place of origination for the monocytes was found to be the kidney, for the lym ...197772711
atrial natriuretic peptide in the eel, anguilla anguilla l.: its cardiac distribution, receptors and actions on isolated branchial cells.the presence of atrial natriuretic peptide (anp) and the nature of its binding sites were studied in fresh-water (fw)- and seawater (sw)-adapted eels using a heterologous analogue, that of the rat (ranp). rat anp-like immunoreactivity was demonstrated in the cardiac atria and ventricles of both fw and sw eels, and electron-dense anp-like granules were observed. the atria and ventricles of fw eels contained significantly more granules than those of sw animals and, in both types, the atria were mo ...19921329803
induction of spermatogenesis and spermiation by a single injection of human chorionic gonadotropin in intact and hypophysectomized immature european eel (anguilla anguilla l.).intact and hypophysectomized male silver eels (anguilla anguilla) in fresh water received a single injection of human chorionic gonadotropin (hcg) (250 c) or solvent (0.15 m nacl). no effect of solvent was observed. spermatogonia proliferated in testis of hcg-treated intact or hypophysectomized eels. one month after the injection, primary and secondary spermatocytes were found. after 3 months, numerous spermatozoa were present. in hypophysectomized eels, hcg was also effective even though maturi ...19873666426
detection of surface carbohydrates on pneumocystis carinii by fluorescein-conjugated lectins.lectins react with a wide range of different carbohydrates (table 1). even so-called monospecific anti-h(o) lectins from lotus tetragonolobus, ulex europaeus, and anguilla anguilla react not only with the anti-h determinant but also with several fucosylated carbohydrates. consequently, the type of lectin receptor existing on the surface of pneumocystis carinii should be determined, because only a carbohydrate analysis can demonstrate the kind of carbohydrates which exist on the cell surface of t ...19873125541
cholecystokinin-pancreozymin-like activity in the eel (anguilla anguilla l.). 19725047345
the effect of mammalian calcitonin on the plasma levels of calcium and inorganic phosphate in the european eel (anguilla anguilla l). 19685674696
[demonstration and determination of oxytocic-type activity of whole homogenates and granular fractions of the preoptico-hypophyseal system in the eel (anguilla anguilla l)]. 19685691923
[groups of transferrins in anguilla anguilla l. phenotypes observed in a population caught on the coast of turkey]. 19684973647
[stimulation of peripheral conversion of thyroxine to triiodothyronine by ovine somatotropic hormone in the eel (anguilla anguilla l.)].one injection of ovine growth hormone (1.27 x 10(-5) usp units/g body weight) determines in the eel, 1 day later, a twofold decrease in plasma thyroxine level as well as a twofold increase in plasma triiodothyronine level. these modifications of circulating iodothyronine levels result from a considerable stimulation of the peripheral deiodination of thyroxine to triiodothyronine, shown by a kinetic study of labelled thyroxine metabolism. the increase of production of metabolically active hormone ...19836405997
endocrine and environmental influences upon plasma cortisol concentrations and plasma renin activity of the eel, anguilla anguilla l.the plasma concentrations of cortisol, sodium, potassium and calcium and plasma osmolarity were determined in freshwater silver eels, after intravascular injections of eel renin preparations, mammalian acth, mammalian angiotensin ii and eel muscle extracts. arterial blood specimens were taken before and after injection of test substances. partially purified eel and rat renal renins gave prolonged pressor responses in intact and hypophysectomized eels and in the nephrectomized rat anaesthetized w ...1976180226
[chromatographic separation and determination of oxytocin type hormones (arginine-vasotocin and isotocin) from the hypophysis and preoptic nucleus of the european eel (anguilla anguilla l.). eels from alsace (auenheim and loire-atlantic (grande-brière)]. 19694981975
suppression of plasma thyroid hormone concentrations by cortisol in the european eel anguilla anguilla.female european eels, anguilla anguilla, were given a single intra-arterial injection via a catheter of cortisol hemisuccinate at doses ranging from 3.5 to 35 micrograms (15 to 150 micrograms/kg body wt), yielding mean plasma cortisol levels of 87-410 ng/ml 2 hr after injection. cortisol treatment (17.5 and 35 micrograms) significantly decreased plasma levels of thyroxine (t4) and triiodothyronine (t3) within 24 hr relative to those in control fish. cortisol treatment (35 micrograms) appeared to ...19862870843
a peptide from the eel pancreas with structural similarity to human pancreatic secretory trypsin inhibitor.the primary structure of a 61-amino-acid residue peptide from the pancreas of the european eel (anguilla anguilla) has been established as e e k s g(5)l y r k p(10)s c g e m(15)s a m h a(20)c p m n f(25)a p v c g(30)t d g n t(35)y p n e c(40)s l c f q(45)r q n t k(50)t d i l i(55)t k d d r(60)c. there was no indication of microheterogeneity. this peptide shows structural similarity to pancreatic secretory trypsin inhibitors from several mammalian species and to a cholecystokinin-releasing peptid ...19883371360
somatostatin-related and glucagon-related peptides with unusual structural features from the european eel (anguilla anguilla).peptides derived from prosomatostatins i and ii and from two distinct proglucagons have been isolated from the pancreas of a teleost fish, the european eel (anguilla anguilla). the product of prosomatostatin i processing, somatostatin-14, is identical to mammalian somatostatin-14. a 25-amino-acid-residue peptide (ser-val-asp-asn-gln5-gln-gly-arg-glu-arg10-lys-ala-gly-cys- lys15-asn-phe-tyr- trp-lys20-gly-pro-thr-ser-cys25) is derived from prosomatostatin ii. compared with the corresponding pepti ...19882904391
co-existence of congeneric species of acanthocephala: acanthocephalus lucii and a. anguillae in eels anguilla anguilla in ireland.a population of eels anguilla anguilla from lough derg, r. shannon, ireland, harbouring infections of both acanthocephalus lucii and a. anguillae was studied over three years. both parasite species had the same intermediate host and eels appeared to be the only definitive host for a. anguillae. throughout the whole period, a. lucii was the dominant parasite, was over-dispersed throughout the eel population and most frequently occurred as a single species infection. a. anguillae was far less comm ...19873696765
daniconema anguillae gen. et sp. n., a new nematode of a new family daniconematidae fam. n. parasitic in european eels.a new nematode genus and species, daniconema anguillae gen. et sp. n., is described from the serosa of the swimbladder and from the intestine of eels (anguilla anguilla (l.] from denmark (lake esrum, northern zealand); a new family daniconematidae fam. n. is established to accommodate it. the hitherto monotypic family daniconematidae shows affinities with the skrjabillanidae; in contrast to the latter, the new family is characterized by the absence of a buccal capsule, by the presence of anus in ...19873428765
the nutrition of the gill parasitic monogenean pseudodactylogyrus anguillae.a histological and histochemical study of ingested food material, energy stores and enzymes in the monogenean pseudodactylogyrus anguillae, parasitizing the gills of the european eel (anguilla anguilla) is presented. it was found that mucus, epithelial cells and blood from the gills were ingested. glycogen deposits were small and primarily located in the parenchyma and to a minor extent in the vitellariae. numerous globules of neutral lipids were found in the vitellariae. a marked esterase activ ...19873422977
[accumulation of cyclic adenosine monophosphate in the ovary of the eel (anguilla anguilla l.) in vitro under the effect of carp gonadotropin or ovine lutropin: kinetics and thermodependence].cyclic amp (camp) in pieces of eel ovary was greatly increased in vitro by the gonadotropin (cgth) of carp, another teleost fish; after one hour at 20 degrees c, maximal stimulation = 31.7 and e.d. 50 = 0.08 micrograms/ml. ovine lutropin (olh) had less effect (maximal stimulation: 2.35; e.d. 50: 1.42 micrograms/ml); its action suggested that it involved a subfraction (olh/cgth rac) of the receptor-adenylate cyclase (rac) systems which mediate the action of cgth. another difference was the percen ...19863012723
effect of decreased oxygen content on eels (anguilla anguilla) infected by anguillicola crassus (nematoda: dracunculoidea).the survival chances of eels with anguillicola crassus infection of varying intensity and with varying pathological changes were studied in an experimental system devoid of fresh oxygen supply. eels most severely affected by anguillicolosis died first, while those with less expressed pathological lesions tolerated sublethal oxygen levels for a longer time. findings were similar at 20-21 degrees c and at 27-28 degrees c; at 27-28 degrees c, however, the fish required a higher oxygen content to su ...19938017238
effects of temperature and dose level on in vivo dynamics of unlabelled bovine insulin in eels (anguilla anguilla l.).the in vivo dynamics of bolus-injected, unlabelled bovine insulin in eels (anguilla anguilla l.), analysed by compartmental and noncompartmental methods, were found to be significantly influenced by acclimation temperature and insulin dose level. at 12 degrees, plasma clearance of insulin was essentially monoexponential, whereas at 22 degrees, the disappearance curves could be resolved into two exponential components. metabolic clearance rate (mcr) and apparent distribution volume (vdist) of ins ...19836341164
[structural similarities between the hypocalcemia hormone of the corpuscles of stannius of the eel (anguilla anguilla l.) and the mammalian parathyroid hormone].extracts of the corpuscules of stannius enhance eel gill calcium efflux and increase bone resorption in the rat. thus the hormone is hypocalcaemic in the eel although hypercalcaemic in the rat. we show here that a 15 min. pulse perfusion of a synthetic fragment of human pth 1-34 had a similar effect on gill calcium fluxes to that of cs extract. further, we found that eel plasma contains a substance reacting with five different antibodies against bovine pth. the concentration of this plasma mater ...19806784952
serological cross-reactivity to rat anti h-y antiserum in the female european eel (anguilla anguilla). 19827106453
[immunocytochemical detection in stannius corpuscles of the eel (anguilla anguilla l.) of a hormone similar to the mammalian parathyroid hormone].in eels the parathyrin of the corpuscles of stannius (pcs), mammalian parathyroid-like hormone, has been localized in the cytoplasm of all the cells in the corpuscles. this detection was done by indirect immunofluorescence with an antiserum anti 1-84 bovine parathormone. the specificity of the reaction was demonstrated by inhibition of the coloration obtained with the 1-84 bovine parathormone and the active fragment 1-34 of human parathormone. variations of the cellular localization of the pcs o ...19816802446
dna dosimetry in biological indicator species living on pah-contaminated soils and sediments.a large variety of environmental carcinogens are metabolically activated to electrophilic metabolites that can bind to nucleic acids, forming covalent adducts. in organisms possessing active metabolic systems for a particular carcinogen, dna adducts generally have longer biological half-lives than the substrate carcinogens. thus, measurement of specific dna adduct concentrations in terrestrial and water organisms may provide a relevant biological indicator of prior exposure to environmental carc ...19957539372
an investigation of the carbonic anhydrase inhibitor in eel plasma.1. carbonic anhydrase activity in blood and gill from freshwater and seawater acclimated european eels, anguilla anguilla has been determined. 2. plasma from freshwater and seawater eels possess a potent inhibitor of erythrocytic carbonic anhydrase, in agreement with previous investigations on freshwater fish. 3. the plasma inhibitor is equally effective against gill and gut carbonic anhydrase from the eel. although not as potent, the eel inhibitor is also effective against ca from rainbow trout ...19836130879
pressor activity in extracts of the corpuscles of stannius from the european eel (anguilla anguilla l.). 19664286639
primary sequence, tissue specificity and expression of the na+,k(+)-atpase alpha 1 subunit in the european eel (anguilla anguilla).the entire cdna nucleotide sequence of the na+,k(+)-atpase alpha 1 isoform was cloned from the gills of the european eel (anguilla anguilla) by a pcr based method. the amino acid sequence translated from the sequence shared 89.4 and 85.6% homology respectively with previously published na+,k(+)-atpase alpha subunit sequences from elasmobranch (torpedo californica) and teleost (catostomus commersoni) fish. the size of na+,k(+)-atpase alpha 1 mrna transcripts in eel tissues was demonstrated to be ...19958574922
immunocytochemical localization of mammalian gnrh (gonadotropin-releasing hormone) and chicken gnrh-ii in the brain of the european silver eel (anguilla anguilla l.).using specific antibodies for the two molecular forms of gonadotropin-releasing hormone (gnrh) present in the european eel, anguilla anguilla, (mammalian gnrh, mgnrh, and chicken gnrh ii, cgnrh-ii), we employed immunocytochemistry to determine the distribution of these two peptides in the brain and in the pituitary. the results indicate that mgnrh and cgnrh-ii are localized in different neurons: mgnrh-immunoreactive (ir) perikaria were observed in the olfactory bulbs, the junction between olfact ...19947873095
the cathodic hemoglobin of anguilla anguilla. amino acid sequence and oxygen equilibria of a reverse bohr effect hemoglobin with high oxygen affinity and high phosphate in other fish, the cathodic hemoglobin of the eel anguilla anguilla is considered to play an important role in oxygen transport under hypoxic and acidotic conditions. in the absence of phosphates this hemoglobin shows a reverse bohr effect and high oxygen affinity, which is strongly modulated over a side ph range by gtp (whose concentration in the red blood cells varies with ambient oxygen availability). gtp obliterates the reverse bohr effects in the cathodic hemoglobin. the molecular basis ...19957642546
third-stage larvae of daniconema anguillae (nematoda: dracunculoidea) in the subcutaneous tissue of eel anguilla anguilla.daniconema anguillae moravec et køie, 1987 larvae measuring 1.64-1.76 mm were occasionally found in considerable numbers in the fins and subcutaneous connective tissue of approximately 50% of eel anguilla anguilla (l.) sampled from lake balaton, hungary. the larvae were noted for their slender body, very long tail with a rounded tip, a densely transversely striated cuticle, and the presence of boring tooth and large kidney-shaped amphids on the cephalic end. the larvae could easily be recovered ...19947883254
[appearance of anguillicola australiensis johnston and mawson, 1940 (dracuncoloidea: anguillicolidae) in anguilla anguilla of lake bracciano].anguillicola australiensis johnston and mawson, 1940 is redescribed and reported for the first time from anguilla anguilla of lake bracciano (rome-italy). it is suggested that the appearance of anguillicola australiensis in european eels might be in relation with intercontinental live eels transfers.19826926929
differential regulation of the two forms of gonadotropin-releasing hormone (mgnrh and cgnrh-ii) by sex steroids in the european female silver eel (anguilla anguilla).the effect of steroids on the two gonadotropin-releasing hormone (gnrh) forms present in the eel (mammalian gnrh, mgnrh and chicken gnrh-ii, cgnrh-ii), as well as on gonadotropin (gth), was studied using specific radioimmunoassays. female silver eels received chronic treatments with various steroids (estradiol, testosterone, androstenedione, 5 alpha-androstane-3 beta, 17 beta-diol). estradiol or the combination of estradiol and androgens induced increases in brain and pituitary mgnrh levels and ...19957617130
present occurrence of anguillicola novaezelandiae (nematoda: dracunculoidea) in europe and its development in the intermediate examination of a sample of european eels, anguilla anguilla (l.), collected from lake bracciano near rome in 1993, the only known european locality with the occurrence of the introduced swimbladder nematode anguillicola novaezelandiae moravec et taraschewski, 1988, revealed for the first time the presence of two anguillicola species, a. novaezelandiae and a. crassus. in view of the investigations carried out by current authors in bracciano lake in the years 1982-1992, it is apparent that the ...19947883252
different effects of cgmp and camp in the intestine of the european eel, anguilla anguilla.the regulation of salt absorption in the sea water eel intestine was studied by evaluating the effects of theophylline, 8 br cyclic adenosine monophosphate, 8 br cyclic guanosine monophosphate, atriopeptin iii, porcine vasoactive intestinal peptide and prostaglandin e1 on the short-circuit current, the transepithelial voltage difference and conductance and on the dilution potentials. it was shown that theophylline increased the transepithelial conductance and reduced the magnitude of the dilutio ...19968621839
fluorescence-labeled fucolectins are superior markers for flow cytometric quantitation of the human sperm acrosome evaluate the binding of three fluorescein (fitc)-labeled fucose-specific lectins, anguilla anguilla agglutinin (aaa), tetragonolobus purpureas agglutinin (tpa), and ulex europaeus-1 agglutinin (uea-1), to unfixed, acrosome-intact and acrosome-reacted (ar) human sperm by flow cytometry and to compare the results with those found using fitc-labeled pisum sativum agglutinin (psa) and arachis hypogaea agglutinin (pna).19968654649
parasitological study of a population of tiber river eels (anguilla anguilla).a study was conducted on the parasitofauna of anguilla anguilla caught in the section of the tiber river south of rome (italy). the sample examined consisted of 417 specimens that were classified by stage (elver, young yellow eel, yellow eel and silver eel) and season of sampling. the following species were identified: trypanosoma granulosum, eimeria anguillae, myxidium giardi, sphaerospora reichenowi, myxobolus sp., trichodina anguilli, ichthyohodo sp., gyrodactylus anguillae, bothriocephalus c ...19873508508
co-localization and secretion of parathyrin of stannius corpuscles (immunoreactive parathyroid hormone) and of secretory glycoproteins including secretory protein-i in the european eel (anguilla anguilla l.).until recently, teleosts were considered to be devoid of parathyroids. we showed recently that the corpuscles of stannius, that structurally have features in common with the parathyroid gland, produce a molecule resembling mammalian parathyroid hormone (pth). we refer to this molecule as parathyrin of corpuscles of stannius (pcs). parathyroid secretory protein-i (sp-i) is an acidic glycoprotein, probably identical to adrenal chromogranin a, that is co-stored and co-secreted with pth. in the pres ...19873504727
habitat specificity of the acanthocephalan acanthocephalus clavula (dujardin, 1845) in eels anguilla anguilla (l).the habitat specificity of the acanthocephalan acanthocephalus clavula in the alimentary tract of eels was studied in natural infections and in fish maintained experimentally under different regimes. the parasite exhibited a preference for a particular region (55 to 65%) of the alimentary canal which did not differ in different species of host, but habitat specificity was not very precise as there was considerable variation between individual hosts and a. calvula was capable of surviving and mat ...19827096967
two pathways for choline transport in eel erythrocytes: a saturable carrier and a volume-activated channel.choline transport in eel (anguilla anguilla) erythrocytes was investigated in cells suspended in isotonic and hypotonic media. in cells in isosmotic solution choline transport was mediated by a saturable system with a michaelis constant (km; 62 +/- 6 microm) similar to that of the choline carrier of human erythrocytes but a maximal transport rate (vmax; 4.5 +/- 0.4 mmol.1 red blood cells-1.h-1) almost two orders of magnitude higher than that in human red blood cells. this pathway was inhibited b ...19948092322
[effect of change of habitat (sea and fresh water) on in vivo thyroglobulin synthesis in atlantic glassed eels (anguilla anguilla l.)].thyroid biosynthesis in glassed eels (anguilla anguilla l.) was studied to establish whether salinity changes could affect it, when they live in sea water or in fresh water containing 125i. aqueous extrait of homogenized cephalic heads of glassed eels contains an iodinated protein 17-19 s having thyroglobulin-like properties and including iodotyrosins (mit and dit) and thyroid hormones (3 and t4). biosynthesis of this proteins is roughly twice more important in fresh water than in sea water at 1 ...19816457669
characterization of the binding specificity of anguilla anguilla agglutinin (aaa) in comparison to ulex europaeus agglutinin i (uea-i).using immunochemical and immunohistochemical methods, the binding site of anguilla anguilla agglutinin (aaa) was characterized and compared with the related fucose-specific lectin from ulex europaeus (uea-i). in solid-phase enzyme-linked immunoassays, the two lectins recognized fuc alpha 1-2gal beta-hsa. aaa additionally cross-reacted with neoglycolipids bearing lacto-n-fucopentaose (lnfp) i [h type 1] and ii [le(a)] and lactodifucotetraose (ldft) as glycan moieties. uea-i, on the other hand, bo ...19968872115
determination of the ca2+ and mg2+ affinity constants of troponin c from eel skeletal muscle and positioning of the single tryptophan in the primary structure.the complete amino acid sequence of troponin c (etnc) from the white muscle of the european eel has been determined by edman degradation procedures. its single tryptophan residue is situated in helix h at amino acid position 152 of the aligned sequence; the tryptophan is the first residue on the c-terminal side of ca2+ binding loop iv. the increase of tryptophan fluorescence emission intensity occurring upon titration of etnc with ca2+ has been used to determine the affinity constants of etnc fo ...19938126218
immunocytochemistry of gonadotropic cells in the pituitary of some teleost antiserum (anti-sgth) raised against salmon gonadotropin (sg-g100) was tested on pituitary sections of sarotherodon mossambicus, carassius auratus, anguilla anguilla (intact and estradiol-treated to induce the development of the gonadotropic (gth) cells), salmo trutta fario, and a protandric marine teleost (sarpa salpa, sparidae). using an immunoenzymologic technique, anti-sgth stained gth and thyrotropic (tsh) cells in the species studied. anti-sgth stained the granulated gth cells in s. tru ...19836862173
some aspects of zinc transport across eel (anguilla anguilla) red blood cell membranes.zinc movement across eel and human red blood cell membranes was measured by atomic absorption spectrophotometry. it was observed that: 1) in human red blood cells zinc uptake is twice as rapid as in fish red blood cells over a temperature range of 10-40 degrees c. the low rate of zinc uptake in eel red blood cell may be simply the side effect of different surface area to volume ratios for the differences in cell size or, it may be due to the low permeability of bicarbonate through the red blood ...19948044143
purification and characterization of the tricarboxylate carrier from eel liver mitochondria.the tricarboxylate carrier from eel (anguilla anguilla) liver mitochondria was solubilized with triton x-100 and purified by sequential chromatography on hydroxyapatite and matrex gel blue b. on sds-polyacrylamide gel electrophoresis, the purified fraction showed a single polypeptide band with an apparent molecular mass of 30.4 kda. when reconstituted into liposomes, the tricarboxylate transport protein catalyzed a very active 1,2,3-benzenetricarboxylate-sensitive citrate/citrate exchange. it wa ...19968687426
angiotensin ii receptor subtypes in eel (anguilla anguilla).previous studies have shown the effects of angiotensin ii (ang ii) in teleosts, and ang ii-binding sites have also been localized in tissues from rainbow trout. the purpose of this study was to extend these findings and to provide an analysis of ang ii receptor (ang ii-r) isoforms in three tissues obtained from european eel (anguilla anguilla). ang ii-rs were identified in eel liver, kidney and intestine membranes by the binding of either 0.5 nmol human 125i-labelled tyr4-ile5-ang ii/l or increa ...19948185815
primary cultures of dispersed pituitary cells from estradiol-pretreated female silver eels (anguilla anguilla l.): immunocytochemical characterization of gonadotropic cells and stimulation of gonadotropin analyze the multihormonal control mechanisms of gth secretion in the eel, primary culture of pituitary cells from control or estradiol-treated female silver eels, a treatment known to stimulate in vivo gth synthesis, was developed. dispersed eel pituitary cells obtained by enzymatic (trypsin/dnase) and mechanical dispersion were cultured in earles m199, at 18 degrees. immunoreactive gth (ir-gth) cells were characterized by the immunoperoxidase method, using antiserum to carp gth beta subunit. ...19968921361
dynamics of anguillicola crassus (nematoda: dracunculoidea) larval infection in paratenic host fishes of lake balaton, hungary.anguillicola crassus, a nematode parasitizing the swimbladder of the european eel (anguilla anguilla) caused substantial mortality among eels in lake balaton in the years 1991 and 1992. parallel to a 3-year study of the infection dynamics of eels, anguillicola larval infection of paratenic host fishes was also surveyed in the lake between 1991 and 1993. during that study, a total of 1,382 specimens of 22 paratenic host fish species were processed. the results showed that anguillicolosis had beco ...19958882739
angiotensin ii stimulation of the basolateral located na+/h+ antiporter in eel (anguilla anguilla) enterocytes.the ph-sensitive fluorescent dye, 2',7'-bis-carboxyethyl-5, 6-carboxyfluorescein acetoxymethyl ester, was used to examine the effects of fish or human angiotensin ii (ang ii) on the activity of the basolateral located na+/h+ antiporter in eel intestinal cell suspensions. exposure of eel enterocytes to either hormone led to an increased activity of the antiporter. this time- and dose-dependent stimulatory effect was inhibited by the specific antiporter inhibitor dimethylamiloride (dma). preincuba ...19968672233
production of recombinant insulin-like growth factor-ii in the development of a radioimmunoassay in rainbow trout (oncorhynchus mykiss).rainbow trout (oncorhynchus mykiss) insulin-like growth factor-ii (igf-ii) cdna was amplified by reverse transcription-polymerase chain reaction and cloned into an expression vector (parhs3) for production by escherichia coli. recombinant rainbow trout igf-ii (rtigf-ii) was recovered from bacterial inclusion bodies, solubilized, and purified by gel filtration chromatography under reducing conditions. after refolding of the peptide by dialysis at basic ph, we obtained a final yield of 820 microgr ...19968930606
genomic variation of aquatic birnaviruses analyzed with restriction fragment length polymorphisms.aquatic birnaviruses are the most ubiquitous and diverse group of viruses in the family birnaviridae. several cause different diseases in a variety of fish species, such as infectious pancreatic necrosis virus in salmonids in north america, europe, and asia and european eel virus in eel in asia. most isolates are antigenically related and belong to a single serogroup (serogroup a) comprising nine serotypes. previous studies with monoclonal antibodies have demonstrated considerable variation in e ...19968779591
localization of rdna genes in european eel (anguilla anguilla) by fish.a probe containing most of the transcription unit of the major ribosomal genes of xenopus laevis has been used to localize the rdna cluster of european eel (anguilla anguilla) by fluorescence in situ hybridization (fish). only on nor-bearing chromosome pair has been found. the results obtained with this technique confirm the size polymorphism of the nor revealed previously by other classical cytogenetic techniques.19968983192
importation of aquaculture seedlings to japan.japan is one of the largest consumers of fishery products in the world. importation of various fishery commodities - including larvae and eggs for aquaculture - is increasing due to high consumer demand. elvers of anguilla anguilla have been imported from europe since the late 1960s. eyed eggs of coho salmon (oncorhynchus kisutch) are imported from north america. during the last decade, over-production caused the price of japanese amberjack (or 'yellowtail') (seriola quinqueradiata) and japanese ...19968890372
potentiating effect of growth hormone on vitellogenin synthesis induced by 17 beta-estradiol in primary culture of female silver eel (anguilla anguilla l.) hepatocytes.previous in vivo experiments have indicated a potentiating role of growth hormone (gh) during experimentally induced vitellogenesis by 17-beta-estradiol (e2) in the female silver eel (anguilla anguilla l.). to investigate whether gh has direct hepatic actions, the effects of hypophysial-purified and recombinant qh on vitellogenin (vg) synthesis in response to e2 were tested on primary cultures of hepatocytes. hepatocytes were prepared from control or e2-primed eels. addition of e2 alone into the ...19968998971
development of a radioimmunoassay for european eel growth hormone and application to the study of silvering and experimental fasting.a specific and sensitive homologous radioimmunoassay for eel (anguilla anguilla l.) growth hormone (anggh) has been developed. the antiserum, raised against purified anggh and used at 1:20,000 final dilution, did not cross-react with eel prolactin or thyrotropin, carp gonadotropin ii, bovine gh, or serum from hypophysectornized eel. the inhibition curves for eel pituitary extracts and serum were parallel to that of anggh standard. the ed50 value was between 1 and 2 ng/tube and the recovery of pu ...19968804566
colonization and establishment of pomphorhynchus laevis (acanthocephala) in an isolated english river.the successful colonization, establishment and spread of pomphorhynchus laevis in a small, isolated, devon river 128 km from the parasite's nearest focus in dorset was followed over 11 years from 1985. the parasite was first detected in anguilla anguilla and platichthys flesus in 1988: by 1995 it had attained prevalence levels of 22.6% in a. anguilla and 43.6% in p. flesus and also occurred in 100% salmo trutta, 50% cottus gobio and noemacheilus barbatulus. as judged by prevalence, abundance, pr ...19968991914
endoparasitic helminths of the european eel, anguilla anguilla, from four disconnected meanders from the rivers leie and scheldt in western flanders, belgium.the endoparasitic helminth communities of the european eel, anguilla anguilla (l.), were investigated in four meanders, cut off from the rivers leie and scheldt in western flanders, belgium. six species of helminths (2 cestodes, 2 nematodes and 2 acanthocephalans) were found. the dominant parasite species was the nematode anguillicola crassus (kuwahara, niimi et itagaki, 1974) infecting 79% of the eel population with intensities up to 112 specimens per fish. at two localities no acanthocephalans ...19979229569
similarities and differences between the massive eel (anguilla anguilla l.) devastations that occurred in lake balaton in 1991 and the past few years, two massive eel (anguilla anguilla l.) devastations occurred in lake balaton, hungary. in 1991, 300 tons of eel perished in the western basin of the lake, while in the summer of 1995 30 tons of eel died in the eastern part of the lake. investigations carried out to find the causes of these ecocatastrophes included measurements of certain biochemical parameters: the blood sugar level, and the acetylcholinesterase (ache, ec, lactate dehydrogenase (ldh, ec, ...19979212331
attempts to analyse anguillicola crassus infection and the humoral host response in eels (anguilla anguilla) of lake balaton, hungary.since the introduction of anguillicola crassus into europe, anguillicolosis has been a considerable problem in several countries. from 1991, periodical eel mortality occurred in lake balaton, hungary. however, eels with a worm burden of 20 to 50 parasites did not show severe swimbladder lesions, which observation cast doubts on the primary aetiological role of the parasite in the eel kill. in order to study the pathology of the infection, from the spring of 1996 until october of the same year, 5 ...19979557321
eel (anguilla anguilla) and brown trout (salmo trutta) target species to assess the biological impact of trace metal pollution in freshwater ecosystems.copper, lead, and cadmium pollution were measured in water and sediments of two northern spanish rivers: piles and pigäeña. liver contents of these heavy metals were analyzed in two fish species (eel, anguilla anguilla, and brown trout, salmo trutta) collected from the same locations. significant levels of heavy metal pollution were found in a 38% of fish that are potential catches for sport fishermen. the results indicate that adult eel could be a good metal bioindicator if sampled at a homogen ...19968854823
changes in gas composition in the swimbladder of the european eel (anguilla anguilla) infected with anguillicola crassus (nematoda).the effect of anguillicola crassus (nematoda) on gas composition in the swimbladder of eels (anguilla anguilla) was studied using mass spectrometry in feral eels from two german rivers and in experimentally infected eels. in both naturally and experimentally infected eels significant correlations were observed between the proportion of oxygen in the swimbladder and level of infection with anguillicola crassus. in swimbladders of naturally infected feral eels the contribution of oxygen to swimbla ...19968851864
down-regulation of tsh subunit mrna levels by thyroid hormones in the european eel.the effects of 3,5,3'-triiodothyronine (t3) and thyroxine (t4) on alpha and thyroid-stimulating hormone (tsh) beta subunit mrna pituitary levels were examined in a teleost, the european silver eel. northern blot analysis showed that the number and length of mrnas encoding tsh beta varied among individuals, a variability apparently not related to thyroidal status. when several bands were present, their intensities were summed for quantitative analysis. increasing circulating thyroid hormones (ths ...19979356215
influence of flumequine and oxytetracycline on the resistance of the european eel against the parasitic swimbladder nematode anguillicola crassus.the effect of the antibacterial drugs flumequine (fq) and oxytetracycline (otc) on the defence system of the european eel (anguilla anguilla l., 1758) was investigated using an experimentally induced infection with the parasitic swimbladder nematode anguillicola crassus. eight weeks after oral administration of infective larvae, the mean recovery of the parasites in fq-treated eels was lower than in non-medicated control animals, and significantly lower than in otc-treated eels. mean numbers of ...19968807782
isolation of six putative p-type atpase beta subunit pcr fragments from the brain of the european eel (anguilla anguilla). 19979432906
early emergence of three dopamine d1 receptor subtypes in vertebrates. molecular phylogenetic, pharmacological, and functional criteria defining d1a, d1b, and d1c receptors in european eel anguilla anguilla.the existence of dopamine d1c and d1d receptors in xenopus and chicken, respectively, challenged the established duality (d1a and d1b) of the dopamine d1 receptor class in vertebrates. to ascertain the molecular diversity of this gene family in early diverging vertebrates, we isolated four receptor-encoding sequences from the european eel anguilla anguilla. molecular phylogeny assigned two receptor sequences (d1a1 and d1a2) to the d1a subtype, and a third receptor to the d1b subtype. additional ...19979006917
heterogeneity among isolates of vibrio vulnificus recovered from eels (anguilla anguilla) in denmark.the findings of this study demonstrate that vibrio vulnificus isolates recovered from diseased eels in denmark are heterogeneous as shown by o serovars, capsule types, ribotyping, phage typing, and plasmid profiling. the study includes 85 v. vulnificus isolates isolated from the gills, intestinal contents, mucus, spleen, and kidneys of eels during five disease outbreaks on two danish eel farms from 1995 to 1997, along with a collection of 12 v. vulnificus reference strains. the results showed th ...19989835548
four polymorphic microsatellite markers in the european eel anguilla anguilla (l.). 19979124719
composition and structure of helminth communities in eels anguilla anguilla from italian coastal lagoons.the composition and diversity of total and intestinal helminth component and infracommunities were determined in eels anguilla anguilla from four shallow coastal lagoons near rome. the lagoons differed principally in respect of their salinity. only lake burano still received an input of freshwater, and both freshwater and marine helminth species were found in eels. in the other three lagoons all helminth species were marine or euryhaline and communities were dominated by digeneans. at component ...19979166436
amphibians and larvae of aquatic insects as new paratenic hosts of anguillicola crassus (nematoda: dracunculoidea), a swimbladder parasite of eels.amphibians (tadpoles of the frog bombina bombina [l.] and the newt triturus vulgaris [l.]) and aquatic insects (larvae of the alderfly sialis lutaria [l.] [megaloptera], dragonflies sympetrum sanguineum [miller] and coenagrion puella [l.] [odonata], and the caddisfly oligotrichia striati [l.] [trichoptera]) were found to serve as paratenic hosts for the third-stage larvae (l3) of the nematode anguillicola crassus kuwahara, niimi et itagaki, 1974, a pathogenic swimbladder parasite of the eel angu ...19989925427
radiodiagnostic method for studying swimbladder inflammation caused by anguillicola crassus (nematoda: dracunculoidea).the nematode anguillicola crassus appeared in europe about 15 yr ago, and has subsequently become recognised as a cause of major pathological lesions in the swimbladder of the european eel anguilla anguilla. the radiographic method reported in this paper showed be a useful complement to diagnostic methods that have so far been based exclusively on fish dissection. using this method, the infection of the swimbladder and the severity of its pathological changes can be assessed in a reliable manner ...19989828409
impact of low water temperature on the development of anguillicola crassus in the final host anguilla anguilla.the effect of low water temperatures on the development and viability of larval and adult anguillicola crassus (nematoda) in the final host anguilla anguilla was studied. european eels were experimentally infected with a. crassus and then maintained for 4 mo at 4, 9, 10, and 19 degrees c. larval development showed a temperature-dependent pattern and was significantly retarded at low temperatures. third-stage larvae survived a 4 mo period at 4 degrees c without being affected, although they were ...19989722403
bioconcentration and excretion of fenitrothion in the brain of the eel (anguilla anguilla).the bioconcentration of fenitrothion in the brain of the european eel (anguilla anguilla) and its posterior elimination have been studied. animals were exposed to a sublethal concentration of fenitrothion (0.04 mg/l) for 96 hours in a flow-through test system. after this pesticide exposure, animals were transferred to clean water for 72 hours more. bioconcentration and elimination processes of fenitrothion were studied in blood and brain. this insecticide showed a strong tendency to bioconcentra ...19979350078
lectin histochemistry reveals the appearance of m-cells in peyer's patches of scid mice after syngeneic normal bone marrow transplantation.peyer's patches in the intestinal mucosa are characterized by the presence of several lymphatic follicles and interfollicular t-cell regions. luminal antigens are transported across the intestinal epithelium to stimulate the peyer's patch pre-b-cells in the follicles that proliferate and migrate to distant sites. evidence suggests that antigen priming of b-lymphocytes is responsible for the number and location of peyer's patches during postnatal life, but little is known about the histogenesis o ...19989446820
distribution, characterization, and growth hormone-releasing activity of pituitary adenylate cyclase-activating polypeptide in the european eel, anguilla anguilla.the complementary dna encoding pituitary adenylate cyclase-activating polypeptide (pacap) has been cloned from two species of teleost fishes, the sockeye salmon and the thai catfish, and the amino acid sequence of pacap has been determined in another teleost, the stargazer. however, to date, the detailed distribution of pacap immunoreactivity has never been investigated in the fish brain. in the present study, we have determined the localization of pacap-immunoreactive neurons in the central ner ...19989751513
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