| isolation of microtubules and a dynein-like mgatpase from unfertilized sea urchin eggs. | taxol was used to prepare microtubules from unfertilized eggs of sea urchins lytechinus pictus, strongylocentrotus droebachiensis , and strongylocentrotus purpuratus. by electron microscopy, these microtubules possessed normal morphology and were decorated with projections. the polypeptides present were tubulin plus microtubule-associated proteins (maps) which included various high molecular weight polypeptides, and a mr = 80,000 polypeptide. these maps were extracted from the microtubules by di ... | 1984 | 6144678 |
| sea urchin tube feet: unique structures that allow a cytological and molecular approach to the study of actin and its gene expression. | actin is the major extractable protein component from the tube feet of four different species of sea urchin: arbacia punctulata, strongylocentrotus purpuratus, strongylocentrotus droebachiensis, and diadema setosum. actin made up as much as 60% of the total coomassie blue-staining material after sds polyacrylamide gel electrophoresis and densitometer analysis. two-dimensional gel electrophoresis resolved two, and possible three, species of actin for each sea urchin of which the dominant componen ... | 1981 | 6894447 |
| n2-fixing vibrios isolated from the gastrointestinal tract of sea urchins. | facultatively anaerobic bacteria, capable of fixing n2 anaerobically or at low o2 concentrations, were isolated from the gastrointestinal tracts of temperate (strongylocentrotus droebachiensis) and tropical (tripneustes ventricosus) sea urchins. morphological and biochemical characteristics, as well as the guanine plus cytosine content of their dna (45.9 and 48.4 mol%), place these isolates in the genus vibrio pacini 1865 in the family vibrionaceae. members of this family have not previously bee ... | 1981 | 6940646 |
| bacterial clearance by the sea urchin, strongylocentrotus droebachiensis. | | 1993 | 8325439 |
| antibacterial activity in strongylocentrotus droebachiensis (echinoidea), cucumaria frondosa (holothuroidea), and asterias rubens (asteroidea). | a search for antibacterial activity in different body parts of the green sea urchin strongylocentrotus droebachiensis, the common starfish asterias rubens, and the sea cucumber cucumaria frondosa was conducted. antibacterial activity was detected in extracts from several tissues in all species tested, but mainly in the coelomocyte and body wall extracts. relatively high antibacterial activity could also be detected in gastrointestinal organs and eggs from a. rubens and in eggs from c. frondosa. ... | 2002 | 12445793 |
| effect of temperature and prey availability on growth of paramoeba invadens in monoxenic culture. | paramoeba invadens jones 1985 is a pathogenic marine amoeba responsible for mass mortalities of sea urchins (strongylocentrotus droebachiensis) of nova scotia between 1980 and 1983. a direct relationship between temperature and sea urchin paramoebiasis has been shown in previous laboratory and field studies. this study examined the effect of prey availability and temperature on the growth of p. invadens in monoxenic culture (with the marine bacterium pseudomonas nautica). at 15 degrees c, the sp ... | 1988 | 16347695 |
| radionuclide concentrations in benthic invertebrates from amchitka and kiska islands in the aleutian chain, alaska. | concentrations of 13 radionuclides (137cs, 129i, 60co, 152eu, 90sr, 99tc, 241am, 238pu, 239,249pu, 234u, 235u, 236u, 238u) were examined in seven species of invertebrates from amchitka and kiska islands, in the aleutian chain of alaska, using gamma spectroscopy, inductively coupled plasma mass spectroscopy, and alpha spectroscopy. amchitka island was the site of three underground nuclear test (1965-1971), and we tested the null hypotheses that there were no differences in radionuclide concentrat ... | 2007 | 17057992 |
| can salinity-induced mortality explain larval vertical distribution with respect to a halocline? | for the larvae of two echinoderm species that coexist in atlantic canada (bipinnaria of the sea star asterias rubens and 4- and 6-arm echinoplutei of the sea urchin strongylocentrotus droebachiensis), we examined the effect of short- and long-term exposure to salinity (ranging from 18 to 35) on the probability of larval survival in laboratory experiments. we also related larval vertical distributions in response to sharp haloclines generated in the laboratory to survival probability in the salin ... | 2008 | 18574109 |
| two recombinant peptides, spstrongylocins 1 and 2, from strongylocentrotus purpuratus, show antimicrobial activity against gram-positive and gram-negative bacteria. | the cysteine-rich strongylocins were the first antimicrobial peptides (amps) discovered from the sea urchin species, strongylocentrotus droebachiensis. homologous putative proteins (called spstrongylocin) were found in the sister species, s. purpuratus. to demonstrate that they exhibit the same antibacterial activity as strongylocins, cdnas encoding the 'mature' peptides (spstrongylocins 1 and 2) were cloned into a direct expression system fusing a protease cleavage site and two purification tag ... | 2010 | 19852980 |
| inhibition of a proteolytic activity from the coelomic fluid of the sea urchin strongylocentrotus droebachiensis by antithrombin iii is potentiated by heparin. | | 2011 | 3303434 |
| anti-infectious and anti-inflammatory effects of peptide fragments sequentially derived from the antimicrobial peptide centrocin 1 isolated from the green sea urchin, strongylocentrotus droebachiensis. | bacterial resistance against antibiotic treatment has become a major threat to public health. antimicrobial peptides (amps) have emerged as promising alternative agents for treatment of infectious diseases. this study characterizes novel synthetic peptides sequentially derived from the amp centrocin 1, isolated from the green sea urchin, for their applicability as anti-infective agents.the microbicidal effect of centrocin 1 heavy chain (cen1 hc-br), its debrominated analogue (cen1 hc), the c-ter ... | 2012 | 23237525 |
| evolution of the rag1-rag2 locus: both proteins came from the same transposon. | the rag1 and rag2 proteins are essential subunits of the v(d)j recombinase that is required for the generation of the enormous variability of antibodies and t-cell receptors in jawed vertebrates. it was demonstrated previously that the 600-aa catalytic core of rag1 evolved from the transposase of the transib superfamily transposons. however, although homologs of rag1 and rag2 genes are adjacent in the purple sea urchin genome, a transposon encoding both proteins so far has not been reported. her ... | 2015 | 25928409 |
| [characteristics of proteinase digestive function in invertebrates--inhabitants of cold seas]. | digestive proteinases of various taxa of invertebrates of the northern seas have been studied: crustaceans paralithodes camtchaticus, pandalus borealis; molluscs chlamys islandicus, buccinum undatum, serripes groenlandicus, and echinoderms strongylocentrotus droebachiensis, cucumaria frondosa, asterias rubens, and grossaster papposus. the presence of two proteolytic activity peaks in the acid (ph 2.5-3.5) and low alkaline zones (ph 7.5-8.5) and a similar proteinase spectrum have been revealed in ... | 2012 | 18038635 |
| seed predation by the shore crab carcinus maenas: a positive feedback preventing eelgrass recovery? | there is an increasing interest to restore the ecosystem services that eelgrass provides, after their continuous worldwide decline. most attempts to restore eelgrass using seeds are challenged by very high seed losses and the reasons for these losses are not all clear. we assess the impact of predation on seed loss and eelgrass establishment, and explore methods to decrease seed loss during restoration in the swedish northwest coast. in a laboratory study we identified three previously undescrib ... | 2016 | 27977802 |
| antiallergic effects of pigments isolated from green sea urchin (strongylocentrotus droebachiensis) shells. | this study was undertaken to evaluate possible antiallergic effects of an extract of pigments from green sea urchin (strongylocentrotus droebachiensis) shells. effects were studied on animal models - guinea pig ileum contraction, rabbit eyes allergic conjunctivitis, and rabbit local skin irritation. the extract significantly reduced, in a dose-dependent manner, the histamine-induced contractions of the isolated guinea pig ileum with id50 =1.2 µg/ml (in equivalents of spinochrome b), had an inhib ... | 2013 | 24288292 |
| the effect of the jessica grounding on subtidal invertebrate and plant communities at the galápagos wreck site. | impacts of the grounding of the oil tanker jessica off san cristóbal island, galápagos, included both effects of oil on biota and also mechanical effects associated with a shallow furrow 50 m x 30 m gouged across the rocky seafloor and wreckage strewn over 7500 m(2). the wreckage represented a minor but potentially chronic source of pollution to the surrounding environment through delayed releases of oil, antifouling compounds and other toxic chemicals, and a possible source of exotic marine tax ... | 2003 | 12810092 |