| arrest of metamorphosis induced by x rays in flesh fly, sarcophaga peregrina. | | 1976 | 948561 |
| selective activation of rna polymerase i in fat body nuclei of sarcophaga peregrina larvae by beta-ecdysone. | | 1976 | 1278592 |
| purification and characterization of a hemocyte proteinase of sarcophaga, possibly participating in elimination of foreign substances. | we have reported that foreign protein injected into the abdominal cavity of sarcophaga peregrina (flesh fly) larvae is degraded in the hemolymph by a proteinase secreted by hemocytes [suzuki, t. and natori, s. (1985) comp. biochem. physiol. 81a, 191-193]. here we report the purification and characterization of a proteinase from larval hemocytes. this enzyme is a cysteine proteinase consisting of 26-kda and 29-kda subunits with similar substrate specificity to mammalian cathepsin b. this enzyme w ... | 1992 | 1425700 |
| crossing between nondiapausing and diapausing races of sarcophaga peregrina. | the temperate race (d) of s. peregrina undergoes pupal diapause in response to certain environmental conditions of photoperiod (13l:11d-11l:13d) and temperature (under 20 degrees c). the tropical race (nd) does not do so under the same circumstances. the tendency toward diapause was suppressed 30-40% of the hybrids of crossings d female x nd male and nd female x dmale even under such short day and low temperature conditions as 11l:13d-9l:15d, 20 degrees c. for entering diapause, the hybrids rreq ... | 1977 | 844547 |
| effects of x rays on histogenesis of abnormal epidermis and age dependency of radiosensitivity during metamorphosis of the flesh fly, sarcophaga peregrina. | | 1978 | 622463 |
| induction of pupal diapause and photoperiodic sensitivity during early development of sarcophaga peregrina larvae. | effects of long day and short day treatments during the embryonic and larval stages on induction of pupal diapause were studied on a diapausing race of sarcophaga peregrina. two long day (15l 9d) cycles during 2 days before or after the larviposition completely stopped the induction of pupal diapause on larvae which grow in short day condition before and after the long day treatment. the sensitivity appeared to decrease during the early stage of the third instar and to increase again to some ext ... | 1979 | 541874 |
| a potent antibacterial protein in royal jelly. purification and determination of the primary structure of royalisin. | a new potent antibacterial protein, for which we propose the name royalisin, was found in royal jelly of the honeybee apis mellifera l. and purified to homogeneity for the first time by acid extraction, gel filtration, and reverse-phase high pressure liquid chromatography. the primary structure of royalisin was determined to consist of 51 residues, with three intramolecular disulfide linkages, having a calculated molecular mass of 5523 da. royalisin is an amphipathic protein, with the c-terminal ... | 1990 | 2358464 |
| effect of cocaine in tissues on the development rate of boettcherisca peregrina (diptera: sarcophagidae). | larvae of the flesh fly, boettcherisca peregrina (robineau-desvoidy), were reared on the tissue of rabbits to study the effects of cocaine and benzoylecognine on development rates. the rabbits were given 35, 69, and 137 mg of cocaine through cardiac puncture. from hours 30 to 70, larvae developed more rapidly on tissue containing cocaine, benzoylecognine, or both, from rabbits injected with 69 and 137 mg of cocaine than on tissue from rabbits injected with 35 mg of cocaine or no cocaine. total d ... | 1989 | 2709390 |
| determination of the disulfide array in sapecin, an antibacterial peptide of sarcophaga peregrina (flesh fly). | sapecin is a 40-residue peptide containing 6 half-cystine residues. the disulfide structure of sapecin was determined by sequencing cystine-containing peptides obtained by digesting sapecin with thermolysin. results showed that sapecin has a vortical structure fixed by 3 disulfide bonds between cysteine residues 3 and 30, 16 and 36, and 20 and 38, respectively, and that these disulfide bonds are essential for its antibacterial activity. | 1990 | 2358424 |
| production of recombinant sarcotoxin ia in bombyx mori cells. | a cdna for sarcotoxin ia, an antibacterial protein of sarcophaga peregrina (fleshfly), was inserted into a silkworm baculovirus vector and expressed in bm-n cells, a line of bombyx mori cells. when a cysteine proteinase inhibitor, p-chloromercuribenzenesulphonic acid, was present in the culture medium, a significant amount of recombinant sarcotoxin ia accumulated, but without this reagent the product seemed to be degraded in this system. the c-terminus of the recombinant sarcotoxin ia seemed to ... | 1990 | 2268291 |
| analysis of a gene cluster for sarcotoxin ii, a group of antibacterial proteins of sarcophaga peregrina. | sarcotoxin ii is a group of antibacterial proteins of sarcophaga peregrina (flesh fly) with related primary structures. we have cloned three genes in this family. these genes formed a tandem array with about 2-kb intervals, and one of them was present in the opposite strand. the putative amino acid sequences of the proteins encoded by these genes were very similar except for a deletion in one of them. all of the genes were found to be activated transiently in the same way when the larval body wa ... | 1990 | 2247051 |
| mode of action of sapecin, a novel antibacterial protein of sarcophaga peregrina (flesh fly). | sapecin is an antibacterial protein purified from the culture medium of nih-sape-4, an embryonic cell line of sarcophaga peregrina [matsuyama, k. & natori, s. (1988) j. biol. chem. 236, 17112-17116]. as this protein inhibited the growth of gram-positive bacteria better than that of gram-negative bacteria, we studied its mode of action with special reference to its effects on s. aureus and escherichia coli. results showed that sapecin had high affinity for cardiolipin, which is a major phospholip ... | 1990 | 2172219 |
| the cecropin locus in drosophila; a compact gene cluster involved in the response to infection. | cecropins are antibacterial peptides that are synthesized in insects as a response to infection. as a first step towards a molecular study of the induction of this response, we have isolated genomic clones that cover the cecropin locus in drosophila melanogaster. this locus was found to be unique, and it was mapped cytologically to the chromosomal location 99e. sequence analysis showed it to be unusually compact, with three expressed genes and two pseudogenes within less than 4 kb of dna, and wi ... | 1990 | 2104802 |
| [anti-tumor factor in insects' hemolymph]. | we have purified a lectin from the hemolymph of sarcophaga peregrina larvae, obtained after injury of their body wall. this lectin recognizes galactose residues and suggested to be involved in the defense mechanism of this insect. we also demonstrated that this lectin induced cytotoxic effects on tumor cells in the presence of murine macrophages. it was found that the murine macrophages had sarcophaga lectin binding proteins. using pupal hemocytes and fatbody of this insect, we established an in ... | 1990 | 2085477 |
| effect of heroin in decomposing tissues on the development rate of boettcherisca peregrina (diptera, sarcophagidae) and implications of this effect on estimation of postmortem intervals using arthropod development patterns. | larvae of the flesh fly boettcherisca peregrina (robineau-desvoidy) were reared on the tissues of rabbits to study the effects of heroin on the development rates of this species. the rabbits were given 6, 12, 18, and 24 mg of heroin by cardiac puncture. from hours 18 to 96, larvae feeding on tissues containing heroin (as morphine) developed more rapidly than those feeding on tissues from the control. the time required for pupation was significantly greater for colonies fed on tissues from heroin ... | 1991 | 2066728 |
| purification of a stage-specific and sequence-specific dna-binding protein for the arylphorin gene of sarcophaga peregrina. | a protein that binds specifically to the nucleotide sequence accacaaca located at residues -247 to -255 upstream of the +1 site of the arylphorin gene of sarcophaga peregrina was purified to homogeneity from fat body nuclei of third instar larvae. this dna-binding protein consisted of two subunits with molecular masses of 40 kda and 42 kda, respectively. accurate transcription initiation of a truncated arylphorin gene in a nuclear extract of nih-sape-4 cells, an embryonic cell line of sarcophaga ... | 1991 | 2025644 |
| involvement of sarcophaga lectin in the development of imaginal discs of sarcophaga peregrina in an autocrine manner. | the imaginal discs of sarcophaga were found not to develop normally in the presence of galactose, a hapten sugar of sarcophaga lectin, or anti-sarcophaga lectin antibody. wing and leg discs cultured with these substances became morphologically abnormal and no imaginal discs reached the stage of terminal differentiation, even in the presence of 20-hydroxyecdysone. the development of the imaginal discs was shown to be autonomously regulated in an autocrine manner by sarcophaga lectin; namely sarco ... | 1991 | 1995404 |
| preferential deadenylation of sarcophaga lectin mrna during its acute phase expression. | the sizes of mrnas of various defense proteins of sarcophaga peregrina (flesh fly) were shown to become progressively shorter. we studied the shortening of the sarcophaga lectin mrna, because this mrna shows acute phase expression on injury of the larvae and programmed expression in the pupal stage. results showed that this mrna underwent deadenylation when expressed in response to body injury, but no appreciable change in size during programmed expression in the pupal stage. during acute phase ... | 1991 | 1989696 |
| drosophila fat body protein p6 and alcohol dehydrogenase are derived from a common ancestral protein. | drosophila melanogaster alcohol dehydrogenase is an example of convergent evolution: it is not related to the adhs of other organisms, but to short-chain dehydrogenases, which until now have been found only in bacteria and in mammalian steroid hormone metabolism. we present evidence that the drosophila adh is phylogenetically more closely related to p6, another highly expressed protein from the fat body of drosophila, than it is to the short-chain dehydrogenases. the polypeptide sequence of p6 w ... | 1991 | 1920455 |
| involvement of sapecin in embryonic cell proliferation of sarcophaga peregrina (flesh fly). | addition of antibodies against sapecin to the culture medium of nih-sape-4 cells derived from a sarcophaga embryo greatly inhibited cell proliferation, whereas addition of sapecin stimulated cell proliferation. these results suggest that sapecin is involved in the proliferation of embryonic cells of sarcophaga. sapecin is known to have potent antibacterial activity, so it seems to have two different biological functions: i.e. protection against bacterial infection and stimulation of embryonic ce ... | 1991 | 1915844 |
| evidence for an increase in positive surface charge and an increase in susceptibility to trypsin of sarcophaga lectin (from the flesh fly, sarcophaga peregrina) on its interaction with galactose, a hapten sugar of the lectin. | when sarcophaga lectin (from the flesh fly, sarcophaga peregrina), an insect humoral lectin, was eluted from a column of deae-cellulose in the presence of galactose (a hapten sugar of this lectin), it emerged at a lower salt concentration than when galactose was absent. in the presence of galactose the lectin was, in addition, more susceptible to trypsin digestion. the lectin was found to have an affinity for basic proteins such as histone h3 and sarcotoxin ia, but this property was lost in the ... | 1992 | 1599400 |
| an infantile case of intestinal myiasis. | a case of intestinal myiasis in an 8-month-old girl is reported. the clinical symptoms were ill humor and mucous bloody stools containing a number of moving worms, which were identified as second-instar larvae of sarcophaga peregrina. | 1992 | 1580159 |
| [mechanism of the dissociation of the fat body at the metamorphosis of sarcophaga peregrina--insect metamorphosis and self-non self recognition]. | | 1992 | 1546165 |
| purification of a 29-kda hemocyte proteinase of sarcophaga peregrina. | previously, we suggested the participation of a hemocyte proteinase in the dissociation of fat body of sarcophaga peregrina (flesh fly) at metamorphosis. we have now purified this proteinase to near homogeneity from pupal hemocytes. it is a cysteine proteinase with a molecular mass of 29 kda and has a unique substrate specificity hydrolyzing both suc-leu-leu-val-tyr-mca and z-phe-arg-mca (suc, succinyl; mca, methylcoumaryl-7-amide; z, carbobenzoxy), which are substrates for chymotrypsin and cath ... | 1992 | 1541301 |
| the 29-kda hemocyte proteinase dissociates fat body at metamorphosis of sarcophaga. | previously, we reported the purification of a 29-kda proteinase from the pupal hemocytes of sarcophaga peregrina (flesh fly). antibody against this proteinase was found to inhibit dissociation of the fat body by pupal hemocytes in vitro. moreover, the purified enzyme alone was shown to dissociate the fat body. this enzyme was found to be localized in granules of heterogeneous size in the hemocytes and to be released on their interaction with the fat body. from these results, we conclude that thi ... | 1992 | 1516741 |
| the fate of the prosegment in the acute-phase and programmed synthesis of sapecin, an antibacterial peptide of the flesh fly (sarcophaga peregrina). | the nucleotide sequence of sapecin cdna suggested that this antibacterial peptide of the flesh fly (sarcophaga peregrina) is produced from preprosapecin by post-translational processing. we examined the production of sapecin and its prosegment by radioimmunoassay under two different physiological conditions in which its gene is activated, assuming that the prosegment has some biological role. results suggested that the prosegment is degraded selectively during production of sapecin. we also foun ... | 1992 | 1445272 |
| purification and characterization of a diptericin homologue from sarcophaga peregrina (flesh fly). | a protein with a molecular mass of 8 kda was found to be synthesized specifically when the fat-body from injured sarcophaga peregrina larvae was cultured in vitro. this protein was purified from the haemolymph of the injured larvae to near-homogeneity. partial amino acid sequencing revealed that this protein is a diptericin homologue. it showed bactericidal activity on growing, but not resting escherichia coli cells. e. coli cells become elongated on treatment with this protein. | 1992 | 1445217 |
| molecular cloning and characterization of the inositol 1,4,5-trisphosphate receptor in drosophila melanogaster. | we isolated a cdna encoding an inositol 1,4,5-trisphosphate receptor (insp3r) of drosophila melanogaster. the predicted drosophila insp3r (2,833 amino acids) has extensive sequence similarity to the mouse insp3r. the polypeptide encoded by the cdna was functionally expressed and showed characteristic insp3-binding activity. the drosophila insp3r gene is located at the region 83a5-9 on the third chromosome and expresses throughout development but predominantly in the adult. localization of the in ... | 1992 | 1322910 |
| uptake of beta-ecdysone by the fat body and membrane vesicles of fat body cells of sarcophaga peregrina larvae. | | 1976 | 1249436 |
| ionophore activity of sarcotoxin i, a bactericidal protein of sarcophaga peregrina. | when escherichia coli was treated with sarcotoxin i, a potent bactericidal protein of sarcophaga peregrina (fleshfly), k+ inside of the cells leaked out rapidly and the atp pool of the cells rapidly decreased. these results suggested that the bactericidal effect of sarcotoxin i was due to its ionophore activity, and that it blocked the generation of atp by inhibiting formation of the proton gradient essential for oxidative phosphorylation. this was confirmed by use of an unca mutant, which was m ... | 1985 | 3899107 |
| antitumor effect of sarcophaga lectin on murine transplanted tumors. | sarcophaga lectin, a lectin purified from the hemolymph of sarcophaga peregrina (flesh fly) larvae, was found to be therapeutically effective against both ascitic and solid tumors. it was especially effective when injected directly into or around nodules of the syngeneic tumor meth a transplanted intradermally into balb/c mice. it also induced cytotoxic activity against l-929 cells when it was added to the culture medium of macrophages in vitro. similar cytotoxic activity was detected transientl ... | 1985 | 3935621 |
| cloning and sequencing of cdna of sarcophaga peregrina humoral lectin induced on injury of the body wall. | a previous paper described the purification of a lectin induced in the hemolymph of larvae of sarcophaga peregrina (flesh-fly) on injury of their body wall (komano, h., mizuno, d., and natori, s. (1980) j. biol. chem. 255, 2919-2924). this paper describes cdna cloning and the complete nucleotide sequence of the gene for sarcophaga lectin. although active lectin consists of alpha and beta subunits in a molar ratio of 2:1, the fat body of injured larvae was found to contain only mrna for the alpha ... | 1985 | 2413021 |
| stage-specific detection of a dna-binding protein for the storage protein gene of sarcophaga peregrina. | a nuclear extract of fat body prepared from third instar larvae of sarcophaga peregrina (flesh fly) was found to contain a dna-binding protein that specifically bound to 5'-upstream region of the storage protein (arylphorin) gene. this protein was found only in larvae harvested 46 h after larval emergence, indicating that its appearance was strictly regulated by the developmental stage of the insect. since the storage protein gene is actively transcribed in the fat body at this stage, this prote ... | 1989 | 2719963 |
| participation of sarcophaga peregrina humoral lectin in the lysis of sheep red blood cells injected into the abdominal cavity of larvae. | sarcophaga lectin which is induced in the hemolymph of sarcophaga peregrina (flesh-fly) larvae when their body wall is injured with a hypodermic needle, was shown to participate in the lysis of sheep red blood cells introduced into the abdominal cavity of the larvae. this finding indicates that humoral lectin plays a role in the defence mechanism of invertebrates. the lysis of sheep red blood cells was enhanced greatly by preinjection of red blood cells, but less by preinjection of other cells. ... | 1985 | 3996706 |
| molecular cloning of cdna for sarcocystatin a and analysis of the expression of the sarcocystatin a gene during development of sarcophaga peregrina. | sarcocystatin a is a cysteine proteinase inhibitor purified from the hemolymph of sarcophaga peregrina larvae [suzuki, t., & natori, s. (1985) j. biol. chem. 260, 5115-5120]. we isolated a cdna clone for sarcocystatin a and analyzed the structure and expression of the sarcocystatin a gene. sarcocystatin a consists of 102 amino acid residues. significant homology was found between amino acid sequences of sarcocystatin a and other mammalian cystatins, and highly conserved sequences among mammalian ... | 1989 | 2785815 |
| structural analysis of a developmentally regulated 25-kda protein gene of sarcophaga peregrina. | in the previous paper, we described the identification of two abundant mrnas of sarcophaga peregrina (flesh-fly) which are selectively expressed in the fat body of middle third instar larvae. one of these mrnas was found to encode a protein with a molecular mass of about 25,000 (25-kda protein) when translated in vitro (tamura, h., et al. (1983) dev. biol. 99, 145-151). present paper reports the nucleotide sequence of a 2.3 kb dna containing the entire gene for the 25-kda protein. this gene cons ... | 1985 | 2993269 |
| [cause of life shortening in adult flesh fly, sarcophaga peregrina]. | | 1973 | 4517303 |
| a possible role of posterior half of larval body on the pupation of the fleshfly, sarcophaga peregrina. | | 1972 | 4537700 |
| induction and termination of pupal diapause in relation to the change of ecdysone titer in the fleshfly, sarcophaga peregrina. | | 1972 | 4541840 |
| effects of ecdysone analogues on development and metabolic activity of wing disks of the fleshfly, sarcophaga peregrina, in vitro. | | 1973 | 4708145 |
| possible involvement of lumichrome in the binding of storage protein to its receptor in sarcophaga peregrina. | previously, a receptor molecule on the surface of fat body cells of sarcophaga peregrina larvae that is involved in the uptake of storage protein from the hemolymph was shown to be a 120-kda protein (ueno, k., and natori, s. (1984) j. biol. chem. 259, 12107-12111). this paper reports evidence that lumichrome (7,8-dimethylalloxazine) may be present in the binding site of the receptor and mediate the binding of storage protein and receptor. a stoichiometric amount of lumichrome was shown to bind t ... | 1987 | 3040764 |
| [radiation effects on the respiration and dna synthesis in the pupa of sarcophaga peregrina]. | | 1967 | 5232505 |
| inhibitory effect of sarcotoxin iia, an antibacterial protein of sarcophaga peregrina, on growth of escherichia coli. | the effect of sarcotoxin iia, an antibacterial protein of sarcophaga peregrina (flesh fly), on escherichia coli was investigated. sarcotoxin iia was found to have a bacterial effect on growing bacteria, but little on non-growing bacteria. at a concentration of 25 micrograms/ml, it induced significant morphological change of growing e. coli cells. in its presence, growing cells became greatly elongated, and spheroplast-like bulges and projections appeared on their surface. a rough mutant strain o ... | 1988 | 3049566 |
| inactivation of alpha-ecdysone and cyasterone by larvae of the fleshfly, sarcophaga peregrina, and pupae of the silkworm, samia cynthia. | | 1970 | 5433302 |
| the effects of amino acids on the labellar hair chemosensory cells of the fly. | the effects of amino acids on the labellar hair chemosensory cells were examined with two kinds of flies (the fleshfly, boettcherisca peregrina, and the blowfly, phormia regina). as a result of this examination, the effects of amino acids were divided into four main classes. amino acids in class 1 did not stimulate any chemoreceptor cell. amino acids in class 2 inhibited nonspecifically the discharges from three kinds of chemosensory cells. amino acids in class 3 stimulated the salt receptor cel ... | 1970 | 5483106 |
| purification of sarcotoxin ii, antibacterial proteins of sarcophaga peregrina (flesh fly) larvae. | three antibacterial proteins with almost identical primary structures termed sarcotoxin iia, iib, and iic were purified to homogeneity from the hemolymph of third instar larvae of sarcophaga peregrina. the molecular masses of these proteins were about 24,000. these proteins were found to have common antigenicity, and antibody against sarcotoxin iia cross-reacted with sarcotoxin iib and iic. radioimmunoassay using this antibody showed that these proteins are induced in the hemolymph in response t ... | 1987 | 3103678 |
| x-ray induced shortening of life span of adult sarcophaga peregrina and its modification by dose fractionation. | | 1965 | 5832640 |
| purification of three antibacterial proteins from the culture medium of nih-sape-4, an embryonic cell line of sarcophaga peregrina. | three antibacterial proteins were purified from the culture medium of nih-sape-4, an embryonic cell line of sarcophaga peregrina (flesh fly). sequencing studies showed that two of these proteins belong to the sarcotoxin i family, potent antibacterial proteins purified from the hemolymph of sarcophaga larvae, whereas the other protein, named sapecin, is a new protein consisting of 40 amino acid residues including 6 cysteine residues. unlike sarcotoxin i, sapecin preferentially represses the growt ... | 1988 | 3182836 |
| molecular cloning of cdna for sapecin and unique expression of the sapecin gene during the development of sarcophaga peregrina. | a cdna clone for sapecin, an antibacterial protein produced by an embryonic cell line of sarcophaga peregrina, was isolated and characterized. this clone was found to encode a precursor of sapecin consisting of 94 residues, with sapecin (40 residues) constituting its carboxyl-terminal half. rna blot hybridization revealed that the gene for the sapecin precursor is activated in the hemocytes of the third instar larvae of sarcophaga in response to body injury. thus, sapecin is probably a defense p ... | 1988 | 3182837 |
| purification of sarcotoxin iii, a new antibacterial protein of sarcophaga peregrina. | a glycine-rich antibacterial protein with a molecular mass of 7,000 termed sarcotoxin iii, was purified to homogeneity from the hemolymph of third instar larvae of sarcophaga peregrina. when the hemolymph was fractionated, this protein was recovered in the same fraction as sarcotoxin i, a group of potent antibacterial proteins that have been purified. but, it was clearly different from sarcotoxin i in amino acid composition and molecular mass. sarcotoxin iii was shown to be induced in the hemoly ... | 1987 | 3312185 |
| novel feature of expression of the sarcotoxin ia gene in development of sarcophaga peregrina. | the expressions of the sarcotoxin ia and iia genes were investigated. although both these antibacterial proteins were synthesized by the fat body and secreted into the hemolymph in the same way when the body wall of third instar larvae of sarcophaga peregrina was injured, the expressions of their genes during development of this insect were different. the sarcotoxin ia gene was activated transiently in the embryonic and pupal stages, whereas the sarcotoxin iia gene was totally inactive in these ... | 1988 | 3342034 |
| humoral mediator-dependent activation of the sarcophaga lectin gene. | sarcophaga lectin is a defence protein synthesized by the fat body and secreted into the hemolymph in response to injury of the body of third instar larvae of sarcophaga peregrina (flesh-fly). in this paper, we demonstrate that the stimulus of body injury is first transmitted to a certain tissue present in the anterior part of the body, and from there a mediator molecule that interacts directly with fat body cells is secreted into the hemolymph. on interaction with this mediator molecule, the fa ... | 1988 | 3366243 |
| purification of sarcophaga (fleshfly) lectin and detection of sarcotoxins in the culture medium of nih-sape-4, an embryonic cell line of sarcophaga peregrina. | an established cell line originating from a sarcophaga peregrina (fleshfly) embryo, nih-sape-4, was found to synthesize mrnas for sarcophaga lectin and sarcotoxin ia, but not those for storage protein or 25 kda protein. these four proteins are known to be synthesized in the fat-body of third-instar larvae, and the two former in particular are known to participate in the defence mechanism of this insect and to be induced in response to injury of the body wall. thus the embryonic cell line nih-sap ... | 1987 | 3435439 |
| a case of intestinal myiasis in japan. | a case of intestinal myiasis caused by larvae of sarcophaga peregrina (japanese name: senchi-nikubae) is reported. the patient passed stools containing live fly larvae for 2 days, but was otherwise asymptomatic. the patient seems to have been infested accidentally as a result of having eaten "yakitori" in which the fly had deposited its larvae. this paper is the first report of intestinal myiasis caused by the 3rd instar of this species. | 1987 | 3508658 |
| interaction between liposomes and sarcotoxin ia, a potent antibacterial protein of sarcophaga peregrina (flesh fly). | the direct interaction between phospholipids and sarcotoxin ia, a potent bactericidal protein of sarcophaga peregrina, was studied using authentic sarcotoxin ia, its synthetic derivatives, and various liposomes. results showed that sarcotoxin ia interacted with liposomes constituted from acidic phospholipids, resulting in the release of glucose trapped in these liposomes. the amidated carboxyl-terminal of this protein was found to be important for this interaction. liposomes constituted from tot ... | 1987 | 3543008 |
| counteraction by 20-hydroxyecdysone of the effect of juvenile hormone on phosphorylation of ribosomal protein s6. | phosphorylation of ribosomal protein s6, induced when the fat body of sarcophaga peregrina (flesh-fly) larvae was incubated in vitro in the presence of 32p, was found to be suppressed by juvenile hormone. this suppressive effect of juvenile hormone was counteracted by a physiological concentration of 20-hydroxyecdysone. since 20-hydroxyecdysone is known to induce phosphorylation of s6 in vivo, this reaction was suggested to be regulated by the balance of the effects of ecdysone and juvenile horm ... | 1987 | 3556581 |
| activation of murine macrophage-like cell line j774.1 by sarcophaga peregrina lectin. | the effect of sarcophaga lectin on mouse macrophage-like cell line j774.1 was studied. it was found that this lectin induced significant morphological changes and stimulated glucose consumption of j774.1 cells. a similar effect was observed when bacterial lipopolysaccharide was added to the culture medium. however, although cells treated with lipopolysaccharide were not cytotoxic to allogeneic tumor cells, those treated with sarcophaga lectin were found to acquire cytotoxicity. the physiological ... | 1984 | 6489679 |
| [biochemistry of sarcophaga peregrina (flesh fly)]. | | 1986 | 3546526 |
| the complete amino acid sequence of echinoidin, a lectin from the coelomic fluid of the sea urchin anthocidaris crassispina. homologies with mammalian and insect lectins. | the complete amino acid sequence of echinoidin, the proposed name for a lectin from the coelomic fluid of the sea urchin anthocidaris crassispina, has been determined by sequencing the peptides obtained from tryptic, staphylococcus aureus v8 protease, chymotryptic, and thermolysin digestions. echinoidin is a multimeric protein (giga, y., sutoh, k., and ikai, a. (1985) biochemistry 24, 4461-4467) whose subunit consists of a total of 147 amino acid residues and one carbohydrate chain attached to s ... | 1987 | 3571253 |
| identification of 30-kda fat body protein of sarcophaga peregrina larvae selectively phosphorylated in the presence of 20-hydroxyecdysone as ribosomal protein s6. | previously, we showed that 20-hydroxyecdysone induces selective phosphorylation of a fat body protein of sarcophaga peregrina with a molecular mass of about 30,000 (30-kda protein) (itoh, k., ueno, k., & natori, s. (1985) biochem. j. 227, 683-688). this paper describes the identification of this 30-kda protein. from the electrophoretic profile of 40s ribosomal proteins on two-dimensional polyacrylamide gel electrophoresis, the 30-kda protein was identified as s6. | 1986 | 3782062 |
| multi-gene structure of the storage protein genes of sarcophaga peregrina. | three recombinant phages containing chromosomal segments of sarcophaga peregrina encoding storage protein were isolated. these clones were found to contain sequences hybridized with the messenger rna of 75,000 mr protein, a monomer of storage protein. from restriction analysis it was found that message-homologous regions are not identical, and thus are different genes with similar sequences. from the analysis of these clones it was concluded that the storage protein gene belongs to a multi-gene ... | 1984 | 6716476 |
| molecular cloning of a cdna and assignment of the c-terminal of sarcotoxin ia, a potent antibacterial protein of sarcophaga peregrina. | a previous paper described the complete amino acid sequences of sarcotoxins ia, ib and ic, which are a group of potent antibacterial proteins with almost identical primary structures produced by sarcophaga peregrina (fleshfly) larvae [okada & natori (1985) j. biol. chem. 260, 7174-7177]. the present paper describes the cdna cloning and complete nucleotide sequencing of a cdna clone for sarcotoxin ia. the c-terminal amino acid residue of sarcotoxin ia deduced from the nucleotide sequence was glyc ... | 1986 | 3827823 |
| sarcophagine (beta-alanyl-l-tyrosine) synthesis in the fat body of sarcophaga peregrina larvae. | the fat body of sarcophaga peregrina larvae was found to have activity for synthesis of sarcophagine (beta-alanyl-l-tyrosine). this activity was due to a soluble enzyme (sarcophagine synthetase) that requires mg2+ and atp as cofactors. the enzyme activity decreased significantly after puparium formation and no sarcophagine synthesis was detected when fat body from white pupae was incubated in vitro with 3h-beta-alanine. this apparent loss of sarcophagine synthesis was found to be partly due to p ... | 1984 | 6746587 |
| purification of lectin induced in the hemolymph of sarcophaga peregrina larvae on injury. | a lectin was purified from the hemolymph of sarcophaga peregrina larvae, obtained after injury of their body wall. this lectin agglutinated sheep red blood cells markedly and the hemagglutinating activity was inhibited by galactose and lactose. the active lectin was found to have a molecular weight of 190,000 and to consist of four alpha subunits and two beta subunits, with molecular weights of 32,000 and 30,000, respectively. during the early pupal stage, similar hemagglutinating activity in th ... | 1980 | 6766942 |
| visual interneurons in the lobula complex of the fleshfly, boettcherisca peregrina. | the physiology and morphology of visual interneurons in the lobula complex of the fleshfly, boettcherisca peregrina, were studied using intracellular recordings and intracellular cobalt stainings, respectively. using responses to movements of a spot of light and on-off stimuli at single positions, we classified the interneurons into five physiological groups on, off, on-off, non-directional motion sensitive (ndm) and directional motion sensitive (dm) neurons. they could be further divided into f ... | 1985 | 3837117 |
| purification and characterization of an inhibitor of the cysteine protease from the hemolymph of sarcophaga peregrina larvae. | the hemolymph of sarcophaga peregrina (flesh fly) larvae was found to contain multiple inhibitors of hemocyte cysteine protease. one of them, named sarcocystatin a, was purified and found to be a mixture of the components sarcocystatin a alpha and a beta in a molar ratio of 2:1. these components can exist in either the associated or dissociated form. the apparent heterogeneity of the protease inhibitors in the hemolymph was found to be partly due to association of sarcocystatin a alpha and a bet ... | 1985 | 3886650 |
| primary structure of sarcotoxin i, an antibacterial protein induced in the hemolymph of sarcophaga peregrina (flesh fly) larvae. | the primary structure of sarcotoxin i, a potent bactericidal protein induced in the hemolymph of larvae of sarcophaga peregrina (flesh fly), was investigated. sarcotoxin i was a mixture of three proteins (sarcotoxins ia, ib, and ic) with almost identical primary structures. these proteins were found to consist of 39 amino acid residues and to differ in only 2-3 amino acid residues. the amino-terminal half of the molecules was rich in charged amino acids and was hydrophilic, whereas the carboxyl- ... | 1985 | 3888997 |
| purification and characterization of an antibacterial protein from haemolymph of sarcophaga peregrina (flesh-fly) larvae. | three antibacterial proteins were induced when the body wall of sarcophaga peregrina (flesh-fly) larvae was injured with a hypodermic needle. these proteins were separated and one was purified to homogeneity. the molecular weight of the purified protein was 5000 and its amino acid composition was similar to that of cecropins, which are antibacterial proteins in hyalophora cecropia (cecropia moth) pupae. this protein was found to have bactericidal activity and to be effective at a concentration o ... | 1983 | 6882366 |
| molecular cloning, sequencing, and characterization of cdna for sarcotoxin iia, an inducible antibacterial protein of sarcophaga peregrina (flesh fly). | a cdna clone for sarcotoxin iia, an antibacterial protein of sarcophaga peregrina (flesh fly) larvae [ando, k., okada, m., & natori, s. (1987) biochemistry 26, 226-230], was isolated and characterized. sarcotoxin iia was found to consist of 270 amino acid residues. northern blot analysis showed that the sarcotoxin iia gene was activated in response to injury of the body wall of the larvae. the gene was activated for much longer after injection of escherichia coli into the abdominal cavity of lar ... | 1988 | 2452654 |
| cloning of gene cluster for sarcotoxin i, antibacterial proteins of sarcophaga peregrina. | a genomic clone of sarcotoxin i was isolated. this clone contained four genes of structurally related proteins belonging to the sarcotoxin i family present in tandem array. one of these genes was sequenced and found to be the sarcotoxin ib gene. this gene contained a single intron of 95 bases. | 1989 | 2689214 |
| induction of selective phosphorylation of a fat body protein of sarcophaga peregrina larvae by 20-hydroxyecdysone. | 20-hydroxyecdysone was shown to induce selective phosphorylation of a fat body protein of sarcophaga peregrina larvae with a molecular mass of 30 kda. this phosphorylation was not associated with synthesis of new protein. fractionation of 32p-labelled fat body by differential centrifugation showed that this protein was mainly present in the membrane-rich fraction, although we could not specify the membrane. thus, 20-hydroxyecdysone may modify the function of the fat body by inducing phosphorylat ... | 1985 | 4004788 |
| changes in head proteins of sarcophaga peregrina with age. | | 1980 | 7358184 |
| effect of ecdysterone contact period on development of wing disks of the fleshfly, sarcophaga peregrina, in vitro. | | 1974 | 4413044 |
| molecular cloning and nuclear localization of atbp, a novel (a+t)-stretch-binding protein of sarcophaga peregrina (flesh fly). | previously, we purified a dna-binding protein, named atbp, from cultured sarcophaga cells. this protein was found to bind specifically to (a+t) stretches that locate in the 5'-upstream region and the intron of the sarcophaga lectin gene [matsui, m., kobayashi, a., kubo, t. & natori, s. (1994) eur. j. biochem. 219, 449-454]. here, we report molecular cloning of a cdna for atbp. the cdna encoded a protein with two zinc-finger motifs, an acidic domain, two glutamine-rich domains and a glutamine run ... | 1995 | 7607207 |
| [radiation induced shortening of life of sarcophaga peregrina and its modification by dose fractionation]. | | 1965 | 5217120 |
| [age-dependent radiosensitivity in adult sarcophaga peregrina]. | | 1968 | 5248445 |
| function of antimicrobial proteins in insects. | we have isolated and characterized various antimicrobial proteins from the haemolymph of sarcophaga peregrina (flesh fly) larvae. of these the sarcotoxin i family is a group of proteins mainly active against gram-negative bacteria whereas sapecin is active mainly against gram-positive bacteria. in addition to its function in defence, sapecin also plays a role in insect development. recently, we identified a hendecapeptide of the sapecin homologue sapecin b that has the same antibacterial activit ... | 1994 | 7768148 |
| toxicity and anticholinesterase activity of two halogenated phosphorothioates to boettcherisca peregrina and chrysomya megacephala. | | 1971 | 5558262 |
| effects of x-irradiation on the development of the respiratory system and survival time in pupae of sarcophaga peregrina. | | 1966 | 5971336 |
| accumulation of cadmium and induction of its binding protein in the digestive tract of fleshfly (sarcophaga peregrina) larvae. | the larva of sarcophaga peregrina ( fleshfly ) was fed with cadmium (cd)-containing diet and the distribution of cd among tissues was determined by separating each organ. approximately 90% of cd accumulated in the larva was found in the digestive tract, the fat body and the malpighian tube being less effective tissues in its accumulation. cd in the digestive tract was mostly bound to an inducible cd-binding protein. the cd-binding protein was a mixture of five isoproteins having several properti ... | 1984 | 6144428 |
| excretion of cadmium and change in the relative ratio of iso-cadmium-binding proteins during metamorphosis of fleshfly (sarcophaga peregrina). | cadmium (cd) was accumulated in the larvae of sarcophaga peregrina (fleshfly) by exposure through diet, and the metabolic fate of the accumulated cd during metamorphosis was examined. most of the cd accumulated in the larvae was retained in the pupae, and 53% of that was excreted from the adults immediately after emergence. the cd-binding protein induced in the larvae was degraded after pupation and the relative ratio of iso-cd-binding proteins re-induced in the adults was different from that in ... | 1984 | 6149071 |
| identification of storage-protein messenger rna of the fleshfly sarcophaga peregrina. | storage-protein mrna was found to be abundant in poly(a)-containing rna extracted from the fat-body of third-instar larvae of sarcophaga peregrina (fleshfly). this rna sedimented at the position of 19s on sucrose-density-gradient centrifugation and the product of its translation in vitro was 75k protein (protein of mol.wt. 75 000), which was precipitated specifically with antibody against storage protein. this product was suggested to contain a signal sequence that is missing in mature storage p ... | 1982 | 6180730 |
| identification and activation of storage protein receptor of sarcophaga peregrina fat body by 20-hydroxyecdysone. | previous work showed that 20-hydroxyecdysone activates the fat body of sarcophaga peregrina larvae to incorporate storage protein selectively from the hemolymph. in this study, storage protein receptors of the fat body membrane which were induced on pupation or on treatment of larval fat body with 20-hydroxyecdysone in vitro were identified. the binding of storage protein to its receptor required divalent cations, especially ca2+, and the binding was very sensitive to ph. the storage protein rec ... | 1983 | 6313643 |
| mode of action of a bactericidal protein induced in the haemolymph of sarcophaga peregrina (flesh-fly) larvae. | the mode of action of a bactericidal protein (sarcotoxin i) purified from the haemolymph of sarcophaga peregrina (flesh-fly) larvae was studied, focusing attention on its effect on the function of the membrane of escherichia coli. sarcotoxin i almost completely blocked the uptakes of tetraphenylphosphonium ion and proline, which are known to be driven by a membrane potential, indicating that this protein disrupts the membrane potential of bacteria. | 1984 | 6383355 |
| induction of human gamma interferon by sarcophaga peregrina humoral lectin. | humoral lectin isolated from the hemolymph of injured sarcophaga peregrina (flesh-fly) larvae was found to activate human peripheral blood cells to produce interferon activity. this interferon was inactivated by dialysis against a solution of ph 2.0 and by heat treatment at 56 degrees c for 30 min, indicating that it was a gamma interferon. the role of this lectin in the defence mechanism is discussed from the viewpoint of comparative immunology. | 1984 | 6434349 |
| identification of storage protein receptor and its precursor in the fat body membrane of sarcophaga peregrina. | a previous paper (ueno, k., ohsawa, f., and natori, s. (1983) j. biol. chem. 258, 12210-12214) showed that a storage protein receptor is present in membranes of the pupal fat body of sarcophaga peregrina, whereas membranes of the larval fat body contain a cryptic receptor, and that 20-hydroxyecdysone is essential for activation of the cryptic receptor to bind and incorporate the storage protein. the present paper reports that the storage protein receptor is a membrane protein of the pupal fat bo ... | 1984 | 6480601 |
| differential expression of two abundant messenger rnas during development of sarcophaga peregrina. | expression of two abundant mrnas in the fat body of third instar larvae of sarcophaga peregrina was studied using genomic clones of storage protein and 25k protein as probes. it was found that these two genes were expressed sequentially in the third instar with a 20-hr interval between their expressions. no amplification of genes was detected during larval development, suggesting that the high level of mrna was due to efficient transcription. the contents of the two mrnas in third instar larvae ... | 1983 | 6617997 |
| polymorphonuclear leukocyte-mediated cytolysis induced by animal lectin. | nine animal lectins, i.e., sarcophaga peregrina agglutinin, balanus roseus agglutinin, aplysia kurodai agglutinin, balanus balanoides agglutinin, tetraclita squamosa japonica agglutinin, misgurnus anguillicaudatus lectin, asterina pectinifera agglutinin, helix aspersa agglutinin and helix pomatia agglutinin, were tested for induction of cytolysis mediated by polymorphonuclear leukocytes. among them, s. peregrina agglutinin and b. roseus agglutinin lysed murine target cells in co-operation with p ... | 1983 | 6628907 |
| sterol-free eukaryotic cells from continuous cell lines of insects. | three cell lines from mamestra brassicae (lepidoptera), one from aedes albopictus (diptera) and one from sarcophaga peregrina (diptera) were continuously cultured in a sterol-free medium and analyzed for sterol content. no sterols were found in any of the cell lines, whereas from cells cultured in a serum-containing medium, cholesterol was detected. this is the first instance of sterol-free animal cells. | 1983 | 6667502 |
| identification of hemagglutinating protein and bactericidal activity in the hemolymph of adult sarcophaga peregrina on injury of the body wall. | when the body wall of adult sarcophaga peregrina (flesh-fly) was injured with a hypodermic needle, hemagglutinating activity and antibacterial activity were induced in the hemolymph simultaneously. the hemagglutinating activity was shown to be due to the same lectin that was found previously in the hemolymph of injured larvae. thus, larval and adult sarcophaga respond in the same way to injury of the body wall. | 1984 | 6734869 |
| a possible mechanism of induction of insect lectin. | studies were made using antibody against a lectin induced in the hemolymph of sarcophaga peregrina larvae on injury. the results suggested that the alpha subunit (mr = 32,000) of the lectin is normally present in the hemolymph. on injury of the body wall, part of the alpha subunit seems to be converted to the beta subunit (mr = 30,000), probably by partial proteolysis with a protease activated in response to the injury. as a result, an active lectin with the structure alpha 4 beta 2 is formed. | 1981 | 6788759 |
| measurement of sarcophaga peregrina lectin under various physiological conditions by radioimmunoassay. | lectin induced in the hemolymph of sarcophaga peregrina (flesh fly) larvae on injury of the body wall or on pupation was studied further by radioimmunoassay, focusing on the interaction between the lectin and hemocytes. it was found that the amount of lectin on the surface of hemocytes prepared from injured larvae increased with time after injury of the body wall. radioiodinated lectin could bind to hemocytes prepared from injured larvae more effectively than those from normal larvae, indicating ... | 1983 | 6822551 |
| change in free amino acids and phospholipids in the head of adult sarcophaga peregrina with age. | the amino acid and phospholipid contents in the head of adult sarcophaga peregrina (flesh-fly) were measured with age. only aspartic acid was found to increase significantly with aging. a significant difference was also detected in the fatty acid composition of the phospholipids. phospholipids isolated from old flies contained more unsaturated fatty acid than young flies, suggesting that the properties of the membrane of head cells change with age. these changes were observed irrespective of sex ... | 1983 | 6873527 |
| analysis of the dnase i-hypersensitive site of a developmentally regulated 25-kda protein gene of sarcophaga peregrina. | change in chromatin structure of a developmentally regulated gene of sarcophaga peregrina (flesh fly) during development was investigated. this gene (25-kda protein gene) was specifically activated in the fat body, but not the hemocytes of larvae in the middle of the third instar. the mrna level in the fat body decreased thereafter, reaching one-fifth of the maximum level in the late third instar to early pupal stage. in the chromatin of fat body nuclei, a dnase i-hypersensitive site was found a ... | 1986 | 3941106 |
| homologies of nucleotide sequences in the 5'-end regions of two developmentally regulated genes of sarcophaga peregrina. | in the previous paper we demonstrated that the storage protein gene and 25-kda protein gene are expressed sequentially in the fat body of middle third-instar sarcophaga peregrina larvae. in this paper, we showed that the expressions of these two genes are regulated at the transcriptional level, and searched for homologous nucleotide sequences in the two genes in the vicinity of their 5'-ends, assuming that these two genes are regulated by a common mechanism. we selected 9 homologous sequences an ... | 1986 | 3960729 |
| [antimicrobial proteins of insect and their clinical application]. | we isolated three antimicrobial proteins from the hemolymph of immunized sarcophaga peregrina (flesh fly) larvae. these are sarcotoxin i, sapecin and antifungal protein (afp). sarcotoxin i and sapecin are highly specific for gram-negative and gram-positive bacteria, respectively. afp was found to have fungicidal activity. primary target for these antimicrobial proteins was assumed to be microbial membrane. we were able to identify a core undecapeptide of sapecin b. we modified this peptide and f ... | 1995 | 7602794 |
| molecular characterization of an insect transferrin and its selective incorporation into eggs during oogenesis. | a protein with a molecular mass of 65 kda that was specifically taken up into eggs was purified from the hemolymph of adult female sarcophaga peregrina flies. from cdna analysis, this protein was shown to be a sarcophaga transferrin. unlike mammalian transferrin, the similarity between its n-terminal and c-terminal halves was only 19%, and it was suggested to conjugate one iron atom/molecule in its n-terminal half. sarcophaga transferrin was found to transport iron ions into eggs during oogenesi ... | 1995 | 7705333 |
| identification of early genes in the drosophila immune response by pcr-based differential display: the attacin a gene and the evolution of attacin-like proteins. | we are using the pcr-based differential display technique to isolate genes which are induced during the immune response in drosophila. in this way, a cdna clone for a member of the attacin family of antibacterial proteins was isolated. the corresponding attacin a (att a) gene is localized at 51a-b on the second chromosome, and it is closely linked to at least one more cross-hybridizing gene. injection of bacteria induces a 0.8 kb transcript, with expression kinetics similar to that of cecropin. ... | 1995 | 7742836 |
| characterization of a bombyx mori cdna encoding a novel member of the attacin family of insect antibacterial proteins. | a bombyx mori cdna was cloned that hybridized with hyalophora cecropia attacin probe and its nucleotide sequence was determined. this cdna consisted of 846 nucleotides and the deduced amino acid sequence showed that the cdna encodes an attacin precursor protein. the putative mature protein of b. mori attacin had 70.4, 68.3 and 18.8% identity in amino acid sequences with that of h. cecropia acidic and basic attacins and sarcophaga peregrina sarcotoxin iia, respectively. b. mori and h. cecropia at ... | 1995 | 7773256 |
| purification and molecular cloning of cdna for an inducible antibacterial protein from larvae of the coleopteran, tenebrio molitor. | antibacterial activity was induced in the hemolymph of larvae of the coleopteran tenebrio molitor by injection of escherichia coli. an antibacterial protein, named tenecin 1, was purified to homogeneity from the larval hemolymph and characterized. a cdna clone for tenecin 1 was isolated and its complete sequence was determined. this protein was found to inhibit the growth of gram-positive bacteria and to consist of 43-amino acid residues including six cysteine residues. the disulfide structure o ... | 1994 | 7798186 |