| maggot therapy: a review of the therapeutic applications of fly larvae in human medicine, especially for treating osteomyelitis. | in traditional medical practice, the larvae of some diptera: calliphoridae, notably lucilia illustris (meigen), l.sericata (meigen) and phormia regina (meigen), have been employed for maggot therapy, i.e. to help clean lesions antiseptically, especially for treatment of chronic osteomyelitis. this mode of treatment remains appropriate for cases where antibiotics are ineffective and surgery impracticable. | 1988 | 2980178 |
| studies on the behaviour of lucilia illustris meigen as a parasite of vertebrates under experimental conditions. | | 1969 | 5770071 |
| a dna-based approach to the identification of insect species used for postmortem interval estimation. | insect larvae found on a corpse can be used for estimating postmortem intervals. here, we describe a molecular method for rapid identification of these insects. specific insect dna fragments were amplified using the polymerase chain reaction (pcr), followed by direct dna sequencing of the amplification products. we sequenced 2300 base pairs of mitochondrial dna from each of three blowfly species: phormia regina, phaenicia sericata and lucilia illustris. all three species are important in forensi ... | 1994 | 8195754 |
| purification and characterization of vitellin-2 from the ovary of the american cockroach, periplaneta americana. | two vitellins (vn-1 and vn-2) were identified in cockroach ovary. vn-2 was purified by ion-exchange chromatography using deae cellulose and sepharose cl-6b, and gel filtration using sephadex g-200 and sepharose 4b. vn-2 comprises three subunits with molecular weights of 102, 72 and 40 kda. vn-2 contains diacylglycerol and triacylglycerol; their fatty acids are mostly lauric acid and palmitoleic acid. vn-2 also has phosphatidylcholine and phosphatidylethanolamine but no sphingomyelin. vn-2 contai ... | 1994 | 8205388 |
| the evolution of ectoparasitism in the genus lucilia (diptera:calliphoridae). | to consider the evolutionary origin of the ectoparasitic habit in the blowfly genus lucilia (diptera:calliphoridae), phylogenetic analyses of mitochondrial dna sequence data were performed for 10 species, including all the common lucilia agents of myiasis, collected from africa, australasia, north america and europe. complementary genetic distance and parsimony analyses are used to consider inter and intraspecific relationships within the genus with reference to previous morphological work. the ... | 1997 | 9076529 |
| seasonal colonization and decomposition of rat carrion in water and on land in an open field in south carolina. | decomposition and insect colonization of rat, rattus rattus l., carrion on land and in water were compared during summer and winter in a plowed field in northwestern south carolina. during winter, carcasses on land reached the dried-remains stage of decomposition, whereas carcasses in water reached the early-floating stage. during summer, carcasses in both habitats entered the final-remains stage of decomposition in 1-2 wk. fewer than 30 species of carrion insects were recorded from the carcasse ... | 1998 | 9775597 |
| partial sequencing of the cytochrome oxydase b subunit gene i: a tool for the identification of european species of blow flies for postmortem interval estimation. | the identification of insects found on a dead body can lead to the estimation of the time of death (postmortem interval). we report an updated version of an established method based on sequence analysis of pcr products from a region of the cytochrome b oxidase subunit i mitochondrial gene of different members of the family calliphoridae, by sequencing six european species: lucilia sericata (meigen), lucilia caesar (linné), lucilia illustris (meigen), calliphora vicina (robineau-desvody), calliph ... | 2000 | 10914577 |
| minimum and maximum development rates of some forensically important calliphoridae (diptera). | blow fly development rates are frequently used to estimate elapsed time since death in homicide investigations in the first few weeks after death. however, in order to make more precise estimates of time since death, accurate developmental data must be generated for all carrion species, and at temperatures that are comparable with those found at crime scenes. this paper presents developmental rates determined for five forensically important species in british columbia, phormia regina (meigen), p ... | 2000 | 10914578 |
| the utility of mitochondrial dna sequences for the identification of forensically important blowflies (diptera: calliphoridae) in southeastern australia. | the applicability of mitochondrial dna (mtdna) sequencing was investigated for the identification of the following forensically important species of blowflies from southeastern australia: calliphora albifrontalis, c. augur, c. dubia, c. hilli hilli, c. maritima, c. stygia, c. vicina, chrysomya rufifacies, ch. varipes and onesia tibialis. all breed in carrion except o. tibialis, which is an earthworm parasitoid. emphasis was placed on calliphora species because they predominate among the carrion- ... | 2001 | 11457611 |
| differences in the ecology of lucilia caesar (l.) and lucilia illustris (meig.) (diptera, callphoridae) in finland. | | 1964 | 14348753 |
| insect fauna visiting carrion in southwest virginia. | successional patterns of insect fauna on pig carcasses were studied in southwest virginia. the objective was to identify and qualitatively assess the major taxa of forensic importance in this region. studies were conducted in spring and summer 2001 and 2002, and fall 2002. over 50 taxa were collected and identified. phormia regina was the dominant fly species in the spring (>90%) and co-dominant with phaenicia coeruleiviridis in the summer. phaenicia sericata, lucilia illustris, and calliphora s ... | 2005 | 15837010 |
| spatial and temporal variability of necrophagous diptera from urban to rural areas. | the spatio-temporal variability of necrophagous fly assemblages in a linear series of habitats from central london to the rural surroundings in the south-west was studied using bottle traps between june 2001 and september 2002. a total of 3,314 individuals in 20 dipteran families were identified from 127 sampling occasions. calliphoridae accounted for 78.6% of all the dipteran specimens, with calliphora vicina robineau-desvoidy, being the most abundant species (2,603 individuals, 46.9%). using c ... | 2005 | 16336303 |
| morphology of the first instar of calliphora vicina, phormia regina and lucilia illustris (diptera, calliphoridae). | scanning electron microscopy documentation of first instar calliphora vicina robineau-desvoidy, phormia regina (meigen) and lucilia illustris (meigen) (diptera: calliphoridae) is presented for the first time, and the following morphological structures are documented: pseudocephalon; antenna; maxillary palpus; facial mask; labial lobe; thoracic and abdominal spinulation; spiracular field; posterior spiracles, and anal pad. light microscopy documentation and illustrations are provided for the ceph ... | 2008 | 18380650 |
| molecular identification of forensically important blowfly species (diptera: calliphoridae) from germany. | forensic entomology applies knowledge about the behaviour and ecology of insects associated to corpses to homicide investigations. it is possible to calculate a minimum post-mortem interval by determining the age of the oldest blowfly larvae feeding on a corpse. the growth rate of the larvae is highly dependent on temperature and also varies between the different blowfly species infesting a corpse. it is, thus, crucial to correctly identify the species collected from a crime scene. to increase t ... | 2009 | 19862555 |
| use of cytochrome c oxidase subunit i (coi) nucleotide sequences for identification of the korean luciliinae fly species (diptera: calliphoridae) in forensic investigations. | blowflies, especially species belonging to the subfamily luciliinae, are the first insects to lay eggs on corpses in korea. fast and accurate species identification has been a key task for forensic entomologists. because conventional morphologic identification methods have many limitations with respect to forensic practice, molecular methods have been proposed to identify fly species of forensic importance. to this end, the authors amplified and sequenced the full length of the cytochrome c oxid ... | 2009 | 19949660 |
| identification of forensically important blowfly species (diptera: calliphoridae) by high-resolution melting pcr analysis. | we describe here the successful coupling of real-time polymerase chain reaction (pcr) and high-resolution melting (hrm) analysis to rapidly identify 15 forensically important species of blowfly from the family calliphoridae (diptera), which occur in poland. two short regions (119 and 70 base pairs, respectively) of cytochrome oxidase gene subunit i with sufficient sequence diversity were selected. in the case of lacking taxa (e.g., reference species) these amplicons can be synthesized using sequ ... | 2010 | 20082091 |
| preliminary studies of the influence of fluctuating temperatures on the development of various forensically relevant flies. | development of larvae of different forensically important flies under constant low, medium and high temperatures as well as under daily fluctuating temperatures in a climatic chamber was investigated and compared. results suggest faster development under fluctuating temperatures for sarcophaga argyrostoma and lucilia illustris but slower development for calliphora vicina and calliphora vomitoria. fluctuating temperatures had a higher impact on larvae in feeding stages than in post-feeding stages ... | 2010 | 20382488 |
| [sequencing of mitochondrial dna co ii gene in carrion-breeding flies and larvae in zhengzhou]. | using co ii sequences to identify common species of carrion-breeding flies and larvae. | 2010 | 20653135 |
| [sequencing of mitochondrial dna cytochrome oxidase subunit i gene in sarcosaphagous flies from 14 provinces in china]. | to detect the 278 bp region of gene of the cytochrome oxidase subunit i (coi) in mitochondral dna (mtdna) of sarcosaphagous flies, identify the species of sarcosaphagous flies, and provide reference for forensic application. | 2010 | 20818074 |
| comparison of decomposition rates and faunal colonization of carrion in indoor and outdoor environments. | decomposition and insect colonization of pig (sus scrofa l.) carcasses were observed over a 42-day period inside and outside a house in a suburban region of edmonton, alberta. three freshly killed pig carcasses were placed outdoors on grass and three carcasses were placed in separate rooms inside a house in a suburban residential area. the carcasses were examined and sampled regularly. outside carcasses were colonized rapidly by calliphora vicina (r-d), lucilia sericata (meigen), lucilia illustr ... | 2011 | 20840295 |
| the use of coi barcodes for molecular identification of forensically important fly species in germany. | deoxyribonucleic acid (dna)-based insect identification has become a routine and accurate tool in forensic entomology. in the present study, we demonstrate the utility of the mitochondrial dna cytochrome oxidase i gene "barcoding region" as a universal marker for molecular identification of forensically important diptera. we analyzed 111 specimens belonging to 13 species originating from frankfurt am main, germany (calliphoridae: calliphora vicina, calliphora vomitoria, lucilia ampullacea, lucil ... | 2011 | 22186975 |
| cuticular muscle attachment sites as a tool for species determination in blowfly larvae. | first results of a new method for species determination in third instar larvae of saprophagous blowflies are introduced. cuticular attachment sites of a limited number of transversal muscles are visualized for light microscopic analysis. after removing the muscles and staining the cuticle, the attachment sites become visible as laterally symmetrical segmental clusters of dark dots. the combined patterns of five such clusters, located in the second, third and fourth segments, show sufficient diff ... | 2011 | 22167366 |
| dataset of traumatic myiasis observed for three dominant screw worm species in north west pakistan with first report of wohlfahrtia magnifica (schiner). | regional surveys were carried out in different parts of north west pakistan among domestic animals (n=57,921) including pets and livestock identifying cases of traumatic myiasis (n=1037). a total of four surveys focused general livestock population during eid ul adha (eid surveys; incidence=1.21%) while another four surveys (miscellaneous surveys; incidence=7.34%) targeted animal population brought to veterinary hospitals and dispensaries. timeframe spanned four years from 2012 to 2015. maggots ... | 2016 | 27570812 |
| decomposition of concealed and exposed porcine remains in the north carolina piedmont. | we examined the decomposition and subsequent insect colonization of small pig carrion (sus scrofa (l.)) placed in concealed and open environments during spring, summer, and fall in raleigh, north carolina, as a model for juvenile human remains. remains were concealed in simulated attics in three manners, ranging from minimal to well-concealed. concealment had a significant effect on the insect community colonizing the remains across all three seasons; the beetles necrobia rufipes (degeer) (cleri ... | 2016 | 26590193 |
| using the developmental gene bicoid to identify species of forensically important blowflies (diptera: calliphoridae). | identifying species of insects used to estimate postmortem interval (pmi) is a major subject in forensic entomology. because forensic insect specimens are morphologically uniform and are obtained at various developmental stages, dna markers are greatly needed. to develop new autosomal dna markers to identify species, partial genomic sequences of the bicoid (bcd) genes, containing the homeobox and its flanking sequences, from 12 blowfly species (aldrichina grahami, calliphora vicina, calliphora l ... | 2013 | 23586044 |
| postfeeding larval dispersal behavior of late season blow flies (calliphoridae) in southern ontario, canada. | postfeeding dispersal involves migration of larvae away from their food source in order to pupate. puparia are difficult to find, yet are important for estimating pmi, and missing puparia during collection can result in inaccurate estimations. this study investigates the late season maggot dispersal patterns for blow flies at coyote carcasses in two habitats with an aim to improving puparia collection procedures. puparia samples collected from various dispersal distances and directions tested th ... | 2014 | 24602116 |
| distribution modeling of three screwworm species in the ecologically diverse landscape of north west pakistan. | north west pakistan (nwp) is characterized by four eco-zones: northern montane region, north western hills, submontane region and indus plains. present study identified 1037 cases of traumatic myiasis in the region during 2012-2015. screw worm larvae were classified as 12 species: chrysomya bezziana (villeneuve), chryomya megacephala (fabricius), chrysomya rufifacies (macquart), lucilia cuprina (wiedemann), lucilia sericata (meigen), lucilia illustris (meigen), lucilia porphyrina (walker), hemip ... | 2016 | 27311386 |
| muscle attachment site (mas) patterns for species determination in european species of lucilia (diptera: calliphoridae). | species identification is generally assessed to be more difficult in larval stages than in adult forms. especially closely related species such as lucilia caesar and lucilia illustris are difficult to identify. the aim of this study was to simplify species determination in lucilia larvae for entomological and forensic purposes. muscle attachment site (mas) patterns were previously found to be a good tool for species determination in blowfly larvae. here, distinctive mas patterns are presented fo ... | 2015 | 25500717 |
| molecular identification of forensically important calliphoridae and sarcophagidae species using its2 nucleotide sequences. | the application of insect evidence to forensic investigations is mainly based on the estimation of postmortem interval and the identification of insect species from samples that are collected from the crime scene. due to the limited number of expert taxonomists, species identification is one of the major barriers for crime scene investigators to utilize forensic entomology. therefore, the molecular identification of species, using mitochondrial cytochrome c oxidase subunit i (coi) gene, has been ... | 2018 | 29331679 |
| development of the green bottle fly lucilia illustris at constant temperatures. | lucilia illustris (meigen 1826) (diptera: calliphoridae) is a cosmopolitan species that commonly colonizes carcasses and occasionally acts as parasites of humans or livestock, making it an insect of significant importance in forensic, medical, and veterinary entomology. however, only a few studies have documented the development of l. illustris. here, we studied the developmental duration and larval body length changes of l. illustris under nine constant temperatures ranging from 15.0 to 35.0°c. ... | 2016 | 27611954 |
| muscle attachment sites of phormia regina (meigen). | an error was found in a previous publication on muscle attachment sites in forensically important blowfly larvae from 2012. the patterns we assigned to lucilia illustris factually belong to phormia regina. | 2014 | 25300418 |
| water balance characteristics of pupae developing in different size maggot masses from six species of forensically important flies. | the impact of maggot mass size on body water content, net transpiration rate, and dehydration tolerance of fly pupae was examined in six species of necrophagous flies. species that spent more time on food as larvae, produced pupae with high body water contents. dehydration tolerance limits of pupae were modest, matching the moisture-rich conditions of decaying carrion for larvae. protophormia terraenovae pupariates on food as it dries, and this was reflected by pupae having the highest body wate ... | 2013 | 23517617 |
| oviposition restraint and developmental alterations in the ectoparasitic wasp, nasonia vitripennis, when utilizing puparia resulting from different size maggot masses of lucilia illustris, protophormia terraenovae, and sarcophaga bullata. | adult females of the ectoparasitoid nasonia vitripennis (walker) are capable of distinguishing between hosts of different quality, and then correspondingly adjust clutch sizes and sex ratios of the offspring. in this study, we examined whether the size of the maggot mass, and presumably the developmental temperature, influenced the suitability of the resulting fly pupal and pharate adult stages as hosts for n. vitripennis. three sizes of maggot masses (100; 500; and 1,000 individuals per mass) w ... | 2012 | 23025195 |