| comparative ability of hybrid and homozygous resistant larvae of lucilia cuprina to cause strikes under experimental conditions. | the intervals between the jetting of sheep with dieldrin or bhc, and the inducement of flystrike by implants of hybrid and homozygous bhc/dieldrin resistant larvae of lucilia cuprina, were compared. flystrikes soon established with homozygous resistant larvae, but considerable protection was afforded against hybrid larvae, the interval being longer with bhc than with dieldrin. dieldrin was shown to have a residual effect on rr larvae in a mixed population 9 weeks after jetting. it is suggested t ... | 1975 | 52354 |
| an in-vitro technique for studying fleece-rot and fly strike in sheep. | fleece-rot was experimentally induced in-vitro by wetting and incubating merino wool samples embedded in serum-agar. gravid lucilia cuprina were readily-attracted to these wool culture plates to oviposit. where serum was freely available to newly-hatched larvae, fly strike and larval development ensued. using this technique, fleece-rot and oviposition were found to be markedly influenced by the availability of protein and by bacterial activity, particularly that of pseudomonas spp. the results i ... | 1978 | 582384 |
| the efficacy of various insecticides against the larvae of lucilia cuprina (wied.), the green blowfly of sheep. i. in vitro tests using a resistant and susceptible strain. | in order to determine the larvicidal action of different insecticides against both susceptible and resistant blowfly strains in the republic of south africa, an attempt was made to find a suitable in vitro method for mortality counts and potency evaluations. after a series of preliminary trials the australian method of roxburgh & shanahan (1973) was adopted as the most reliable for this purpose. a statistical analysis by computer of the basic data at onderstepoort established the superiority of ... | 1978 | 750957 |
| evidence of a calcium-ion-transport system in mitochondria isolated from flight muscle of the developing sheep blowfly lucilia cuprina. | the egta (ethanedioxybis(ethylamine)tetra-acetic acid)-ruthenium red-quench technique (reed & bygrave, 1974a) was used to measure initial rates of ca-2+ transport in mitochondria from flight muscle of the blowfly lucilia cuprina. evidence is provided for the existence in these mitochondria of a ca-2+-transport system that has many features in common with that known to exist in rat liver mitochondria. these include requirement for energy, saturation at high concentrations of ca-2+, a sigmoidal re ... | 1975 | 807204 |
| isolation and characterization of ribosomes from thoraces of the sheep blowfly, lucilia cuprina, and their capacity for protein synthesis at different stages of development. | | 1975 | 1116644 |
| synchronous appearance of adenine nucleotide translocase activity and oxidative phosphorylation in mitochondria from flight-muscle of the developing sheep blowfly, lucilia cuprina. | | 1975 | 1132511 |
| resistance of the sheep blowfly lucilia cuprina to insecticides in the republic of south africa. | laboratory tests on larvae of nine strains of the sheep blowfly, lucilia cuprina, and one of the hairy blowfly, chrysomia albiceps, taken from the field are reported. results indicate resistance to arsenic in one strain and to diazinon in the remainder and in chrysomia albiceps. field evidence corroborates the presence of resistance in the blowfly maggots to current flystrike insecticides with reduced protection resulting in more frequent treatment. | 1975 | 1223284 |
| enzymes in saliva from four parasitic arthropods. | enzyme assays and sds polyacrylamide gel electrophoresis were carried out on saliva and in some cases homogenates of salivary gland and gut from four parasitic arthropods (the cattle tick, boophilus microplus (canestrini); the mosquito, aedes aegypti (l.); non-parasitic adult and parasitic larval blowfly of sheep, lucilia cuprina (wiedemann); the buffalo fly, haematobia irritans exigua de meijere). saliva from all species showed large differences in the number and molecular weight of components, ... | 1992 | 1330086 |
| genomic sequences with homology to the p element of drosophila melanogaster occur in the blowfly lucilia cuprina. | we have cloned two dna elements (lu-p1 and lu-p2) from the australian sheep blowfly lucilia cuprina that are similar to the transposable p element of drosophila melanogaster in both structure and sequence but have diverged from it and from each other considerably. hybridization studies indicate that a third related element probably exists in another, as yet unsequenced, clone. neither lu-p1 nor lu-p2 appears to be active in terms of mobility, and it is not known whether any transposition-compete ... | 1992 | 1332056 |
| the synthesis of compound autosomes in the australian sheep blowfly lucilia cuprina. | the synthesis of the first compound chromosome strain in lucilla cuprina (diptera: calliphoridae) (weidemann) is described. crosses between the compound chromosome strain and a chromosomally normal strain produce no offspring, whereas crosses between compound chromosome-bearing individuals are fertile. cytological evidence is presented that compound chromosome arms assort art random with respect to one another during spermatogenesis. | 1976 | 938976 |
| dietary-induced modification of calcium transport in mitochondria isolated from flight-muscle of developing sheep blowfly lucilia cuprina. | | 1977 | 921793 |
| ovarian development in autogenous and anautogenous lucilia cuprina in relation to protein storage in the larval fat body. | | 1977 | 886196 |
| inability of tributyltin-induced chloride/hydroxyl exchange to stimulate calcium transport in mitochondria isolated from flight muscle of the sheep blowfly lucilia cuprina. | tributyltin in the concentration range 1-4mum failed to stimulate ca(2+) transport by lucilia flight-muscle mitochondria in a medium containing kcl and respiratory substrate but devoid of p(i), despite its promotion of a rapid cl(-)/oh(-) exchange. when 2mm-p(i) was present, concentrations of tributyltin greater than 1mum inhibited the initial rate of ca(2+) transport and induced efflux of the ion from the mitochondria in cl(-)- or no(3) (-)-containing media. lower concentrations had little effe ... | 1978 | 728076 |
| chemosterilization of lucilia cuprina (diptera: calliphoridae) with p,p'-bis(1-aziridinyl)-n-cyclohexylphosphinothioic amide. | | 1979 | 541805 |
| genetics of the hemolymph esterases of lucilia cuprina (diptera: calliphoridae). | when hemolymph from adults of lucilia cuprina was partitioned on native polyacrylamide gels, nonspecific esterase staining demonstrated 10 bands with up to six bands in an individual. the bands derive from alleles at two loci, eha (five alleles) and ehb (four alleles). eha is located on chromosome 4, 16.3 map units from sv (singed vibrissae) and 22.1 map units from ra (radial vein gaps). ehb is located on chromosome 5, 34.0 map units from to2 (topaz2 eyes) and 7.2 map units from mv (m1-veinless) ... | 1992 | 1590744 |
| the blowfly strike problem of sheep in new south wales. | a field survey was undertaken between 1972-76 to reappraise the nature of the blowfly problem in new south wales. for 2 years, 1972-1974, some 80,000 sheep were kept under observation and 12,481 strikes were reported, most due to lucilia cuprina. breech strike was still the basic problem but tail strike associated with scouring had become an important component where pastures have been improved. breech strike was controlled at minimal cost by managerial practices such as docking tails the correc ... | 1979 | 518435 |
| enrichment of ruthenium red-sensitive ca2+ transport in a population of heavy mitochondria isolated from flight-muscle of lucilia cuprina. further evidence for its heterogeneous distribution in the inner mitochondrial membrane. | | 1978 | 720623 |
| laval implant sutdies with lucilia cuprina. | semi-field studies, using the larval implant technique, show that sheep may be protected against resistant larvae of lucilia cuprina by thorough jetting with chlorfenvinphos, diazinon, dichlofenthion and fenthion ethyl, but not bromophos ethyl, for the normal duration of waves of bodystrike in new south wales. the standard method of assessing implant data is to be preferred to analyses based on average protection due to the extra time required for the latter procedure. | 1978 | 318562 |
| effects of cyromazine on reproduction and offspring development in lucilia cuprina (diptera: calliphoridae). | the effects of cyromazine on reproduction and subsequent hatch and larval developmental in lucilia cuprina (wiedemann) were examined by feeding the compound in water to adult flies at concentrations up to 100 ppm. cyromazine did not interfere with oviposition or hatch; however, subsequent larval development was strongly inhibited in a concentration-dependent manner. | 1992 | 1401479 |
| xanthommatin biosynthesis in wild-type and mutant strains of the australian sheep blowfly lucilia cuprina. | the synthesis of eye pigments has been studied in the seven eye color mutants of the australian sheep blowfly, lucilia cuprina. six appears to be affected primarily in the synthesis of xanthommatin. in wild type, the onset of xanthommatin biosynthesis occurs midway through metamorphosis. developmental patterns of accumulation of the xanthommatin precursors tryptophan, kynurenine, and 3-hydroxykynurenine have also been established for wild type. by determining the levels of these precursors in la ... | 1978 | 751645 |
| effect of antibacterial inhibitors on protein synthesis in isolated flight muscle mitochondria of the blowfly lucilia cuprina: phylogenetic implications. | | 1976 | 1278433 |
| selection of dieldrin-resistant strains of lucilia cuprina (diptera: calliphoridae) after ethyl methanesulfonate mutagenesis of a susceptible strain. | after ethyl methanesulfonate (ems) mutagenesis of a susceptible strain (swt), selective screening of lucilia cuprina (wiedemann) resulted in four strains that were resistant to the insecticide dieldrin. concentrations used for selection were greater than lc99 of susceptible phenotypes. no resistant variants were screened from the standard laboratory strain (swt) not treated with ems. the resistance phenotypes of the four resistant strains were similar to each other and to that of a field-selecte ... | 1992 | 1593011 |
| comparative effectiveness of avermectins and deltamethrin in suppressing oviposition in lucilia cuprina (diptera: calliphoridae). | the activities of three avermectins and deltamethrin as oviposition suppressants were investigated with a laboratory bioassay in which gravid females of the blowfly lucilia cuprina (wiedemann) were exposed to treated oviposition targets. an easily comparable index of suppression, the oviposition suppression concentration (osc), was defined. all four compounds were effective oviposition suppressants. the three avermectins had similar osc50 values (approximately 13 ppm). deltamethrin, with an osc5 ... | 1992 | 1607479 |
| the amino acid sequence of cytochrome c from the blowfly lucilia cuprina. | the amino acid sequence of cytochrome c isolated from the sheep blowfly lucilia cuprina has been determined by comparison of the compositions of the tryptic peptides to those predicted from the published sequences of cytochromes c from other insects. cytochrome c from l. cuprina differs at a single residue when compared to cytochrome c from the screw worm fly haematobia irritans, a species belonging to the same order as the blowfly. this substitution, proline for alanine, has been located at pos ... | 1978 | 202328 |
| genetics of lucilin, a storage protein from the sheep blowfly, lucilia cuprina (calliphoridae). | lucilin, the main storage protein of larval fat body and hemolymph in the sheep blowfly, lucilia cuprina, has been isolated as a series of trimers composed of subunits of 83,000 +/- 5%, daltons. extensive electrophoretically detectable polymorphism of lucilin subunit patterns occurs in wild and laboratory populations of lucilia; from four to nine bands are seen in any one individual. evidence from genetic, electrophoretic, immunological, and structural studies suggests the existence of a series ... | 1976 | 1259701 |
| cloning and characterization of the white and topaz eye color genes from the sheep blowfly lucilia cuprina. | clones carrying the white and topaz eye color genes have been isolated from genomic dna libraries of the blowfly lucilia cuprina using cloned dna from the homologous white and scarlet genes, respectively, of drosophila melanogaster as probes. on the basis of hybridization studies using adjacent restriction fragments, homologous fragments were found to be colinear between the genes from the two species. the nucleotide sequence of a short region of the white gene of l. cuprina has been determined, ... | 1990 | 1971656 |
| association of exogenous dna with cattle and insect spermatozoa in vitro. | spermatozoa isolated from domestic cattle (bos taurus), the australian sheep blowfly (lucilia cuprina), and the honeybee (apis mellifera) are capable of binding exogenous radiolabeled linear dna. both motile and nonmotile bovine sperm exhibit four distinct patterns of dna association. following treatment with dnase i, the relative proportion of one of these patterns increases specifically in living sperm, suggesting that a small proportion of dna that associates with bovine sperm may be sequeste ... | 1991 | 2054177 |
| polygenic and single gene responses to selection for resistance to diazinon in lucilia cuprina. | following mutagenesis with ethyl methanesulfonate, selection in a susceptible strain with a concentration of the insecticide diazinon (0.0004%, w/v) above that required to kill 100% of the susceptible strain, the lc100 of that strain, resulted in a single gene response. the resultant four mutant resistant strains have equivalent physiological, genetical and biochemical profiles to a diazinon-resistant strain derived from a natural population and homozygous for the rop-1 allele. modification of t ... | 1992 | 1551581 |
| characterization and chromosomal distribution of a tandemly repeated dna sequence from the australian sheep blowfly, lucilia cuprina. | in the course of making a lucilia cuprina genomic dna library, a ladder of bands was seen in partial sau3a digests. complete digestion reduced this ladder to predominantly monomer units of approximately 190 bp. nine independently isolated copies of this repeat were cloned and sequenced. only two of these isolates are identical in sequence, the most divergent being 71% homologous. this satellite dna occurs in all three wild-type strains tested, and, for the single case examined, in the embryonic, ... | 1992 | 1576886 |
| hypersensitivity responses and repeated infections with lucilia cuprina, the sheep blowfly. | sheep repeatedly infected with l. cuprina at 2- but not 4-week intervals developed partial resistance to infection after five infections, as measured by larval recovery. however, resistance did not persist for more than three infections. skin weal responses were measured after injection of larval products simultaneously with each infection. the only correlation between weal size and larval recoveries occurred at infection 1 and indicated a relationship between skin sensitivity and innate rather ... | 1992 | 1487378 |
| the sheep antibody response to repeated infection with lucilia cuprina. | the specific serum antibody responses of sheep exposed to 10 consecutive infections of l. cuprina have been analysed by enzyme-linked immuno-sorbent assay and immunoblotting using monoclonal antibodies specific for sheep immunoglobulin isotypes. recognition of a number of larval excretory-secretory products by igm antibodies appeared to be non-specific. igg1 was the major antibody class stimulated by the infection protocol and marked increases in antibody to specific excretory-secretory antigens ... | 1992 | 1487377 |
| fatal association between dieldrin-resistant and susceptible australian sheep blowflies, lucilia cuprina. | a novel phenomenon of interactions between genotypes of the dieldrin-resistance (rdl) locus of the australian sheep blowfly (lucilia cuprina) is described. susceptible adult flies exposed to dieldrin-resistant (rdl/rdl or rdl/s) adults, raised from larvae grown on media containing sublethal concentrations of dieldrin, display mortality related to the concentration on which the resistant flies developed. the resistant flies excrete quantities of dieldrin that are toxic to susceptible flies. these ... | 1992 | 1349179 |
| susceptibility of mulesing wounds to oviposition and strike development by the australian sheep blowfly lucilia cuprina. | the age and/or physical condition of mulesing wounds had a significant effect on both the oviposition response of l. cuprina and the ability of the wound to support a strike. up to 48 h after mulesing, untreated wounds elicited a strong oviposition response in contrast with chemically treated wounds, although subsequent larval development was negligible. seven to 9-day-old wounds, however, regardless of the wound treatment, were highly attractive oviposition sites, which subsequently developed i ... | 1990 | 2288537 |
| reduced growth of lucilia cuprina larvae fed serum from sheep treated with anthelmintics. | the effect of three commonly used anthelmintics, levamisole hydrochloride, ivermectin and closantel, on the development of the sheep blowfly, lucilia cuprina, was determined. sheep were treated with each anthelmintic using the manufacturers' recommended dose for helminth control. both ivermectin and closantel significantly (p < 0.05) reduced the growth rate of larvae of l cuprina cultured in vitro on serum from these sheep. levamisole hydrochloride had no effect. ivermectin was effective for les ... | 1992 | 1288476 |
| hormonal control of morphogenetic cell death of the wing hypodermis in lucilia cuprina. | 1. cytoplasmic fragments in the haemolymph of newly emerged flies derive from the degenerating wing hypodermis. 2. at the time of eclosion, dorsal and ventral cell layers of the wing are connected by processes containing bundles of microtubules and microfilaments. cytoplasmic fragments contain similar bundles of microtubules but few microfilaments. 3. extensive vacuolation marks the onset of hormonally initiated fragmentation of the wing hypodermal cells. haemocytes containing lysosomes are pres ... | 1975 | 1145608 |
| acquired resistance of sheep to larvae of lucilia cuprina, assessed in vivo and in vitro. | the effect on subsequent larval survival of infesting sheep repeatedly with larvae of lucilia cuprina was assayed in vivo and in vitro. one in vivo assay technique, in which implanted larvae were grown to third instar, indicated a significant reduction in larval survival; another in vivo technique, in which larvae were allowed to develop to second instar in small aluminium rings attached to the sheep, indicated no reduction in larval growth or survival. larvae of lucilia cuprina grown in vitro o ... | 1990 | 2358312 |
| cutaneous myiasis. recent advances in biology, immunology and improvements of the control measures. | the evolution of the main topics of study related to the biology, the life-cycle and the distribution of insects causing cutaneous myiasis during the last century is presented. these subjects are still of interest and a synopsis of new data concerning hypoderma tarandi, h. diana and prezhevalskiana silenus is reported as well as the changes of distribution of some species such as cochliomyia hominivorax. in the second part, an over view of what has been archived on myiasis immunology is reviewed ... | 1991 | 1805677 |
| cellular immune responses in the skin of sheep infected with larvae of lucilia cuprina, the sheep blowfly. | cellular immune responses were observed in the skin of sheep after primary and secondary infection with sheep blowfly (lucilia cuprina) larvae. both primary and secondary infections resulted in a massive cellular infiltration within 48 h of wound initiation, with the majority of cells having the cd45 phenotype. neutrophils comprised the major cell type at the skin surface. in the dermis, the number of cd4+ t helper, gamma delta-tcr+ cells and t19+ (cd4-, cd8-) t cells also increased significantl ... | 1992 | 1441187 |
| vaccines against blowfly strike: the effect of adjuvant type on vaccine effectiveness. | vaccination of sheep with a partially purified extract of lucilia cuprina larvae in some cases resulted in marked reduction of growth in larvae which fed on the sheep. twelve adjuvants were assessed, in vitro and in vivo, to determine which induced the largest inhibitory effect on larval growth. the freund's complete adjuvant and quil a groups produced elisa antibody levels significantly higher (p less than 0.05) than other groups. seven adjuvants mediated an immune response which caused signifi ... | 1992 | 1639566 |
| the immune response of the sheep popliteal lymph node to a purified phenoloxidase from larval cuticle of the sheep ectoparasite, lucilia cuprina. | the popliteal lymph node of the sheep can mount a strong immune response to a phenoloxidase, enzyme a, purified from larval cuticle of a major sheep ectoparasite, the sheep blowfly, lucilia cuprina. continuous sampling from a cannulated efferent lymphatic allowed monitoring of changes in both cellular output (total cells and large blast cells) and specific antibody production (by elisa) following primary and secondary challenge with antigen. anti-enzyme a antibodies in lymph selectively precipit ... | 1989 | 2493091 |
| monitoring and selection of resistance to pyrethroids in the australian sheep blowfly, lucilia cuprina. | field and laboratory populations of the australian sheep blowfly, lucilia cuprina (wiedemann) (calliphoridae), were surveyed by bioassay for possible resistance to the synthetic pyrethroids, a group of insecticides under development for blowfly control. a normal distribution of lc50 values was found using deltamethrin, the test pyrethroid, with no indication of specific resistance despite widespread use of deltamethrin on sheep to control the sheep body louse, damalinia ovis (schrank) (trichodec ... | 1989 | 2519674 |
| gold labelling of rna in virus-induced mitochondrial vesicles in the sheep blowfly lucilia cuprina. | membranous vesicles are thought to be the replication site for viral rna of many plant and animal viruses. a relatively rare site of virus-associated vesiculation is that of the mitochondrial outer membrane. in this study, virus-induced mitochondrial vesicles of the blowfly, lucilia cuprina, were labelled with ribonuclease/gold and an antibody against double stranded rna (anti-polyinosinic:polycytidylic acid). both methods showed the presence of rna in the vesicles thus indicating they may be a ... | 1992 | 1378980 |
| intraruminal controlled release of cyromazine for the prevention of lucilia cuprina myiasis in sheep. | the efficacy of cyromazine, continuously released from intraruminal capsules at dose rates from 0.5 to 2 mg kg-1 d-1, was evaluated against implants of eggs and first instar larvae of lucilia cuprina on merino sheep. estimates from the non-linear relationship between the success of implants and plasma concentrations showed that 95 per cent protection of sheep could be achieved at a mean release rate of 1.39 mg kg-1 d-1 cyromazine which gave rise to a mean plasma concentration of 0.26 mg litre-1. ... | 1989 | 2704878 |
| tryptic and chymotryptic proteases released by larvae of the blowfly, lucilia cuprina. | proteases released by larvae of the sheep blowfly have been suggested to have a primary role in wound formation and larval nutrition. assays were carried out on two larval products to analyse the substrate specificity of these proteases, their abundance and approximate molecular weights. tryptic and chymotryptic activities were found in both products though there were more chymotrypsin-like enzymes in products from 48 h cultures (cesp) than in product collected direct from 48 h larvae (lesp). so ... | 1990 | 2074126 |
| further larval implant studies with lucilia cuprina. | | 1975 | 1146180 |
| occupational allergy in an entomological research centre. i. clinical aspects of reactions to the sheep blowfly lucilia cuprina. | twenty eight per cent (15) of 53 workers engaged in a sheep blowfly breeding programme designed to control genetically the pest lucilia cuprina experienced allergic manifestations resulting from contact with this insect. the most common symptoms were rhinitis, affected eyes, rashes, and lower respiratory symptoms, usually, but not always, immediate in type. a personal history of non-insect related asthma, allergic rhinitis, or eczema, or a combination of these was more common in the fly allergic ... | 1989 | 2765420 |
| salivary gland chromosomes of lucilia cuprina (diptera, caliphoridae). | the salivary gland chromosome complement in lucilia cuprina consists of five pairs of autosomes and two sex chromosomes x and y, all with characteristic banding patterns. a standard map for the salivary gland chromosomes of this species has been prepared, and compared with the known map of lucilia cuprina dorsalis from australia. the differences in their banding patterns indicate the existence of different varieties within the same species. | 1976 | 1031663 |
| sheep plasma protease inhibitors influencing protease activity and growth of lucilia cuprina larvae in vitro. | the enzyme inhibitors alpha 2-macroglobulin (alpha 2m), anti-thrombin iii (at iii) and alpha 1-proteinase inhibitor (alpha 1pi) were isolated from sheep plasma and tested for their ability to affect l. cuprina larval proteases and larval growth in vitro. casein radial diffusion gels indicated that both alpha 2m and alpha 1pi completely inhibited the protease activity of a larval excretory-secretory preparation, while at iii had a partial effect. casein zymograms revealed that alpha 2m inhibited ... | 1990 | 2185168 |
| prospects for the control of sheep blowfly strike by vaccination. | research into vaccination against flystrike is aimed at either controlling the predisposing condition, fleece rot, or direct control of the fly maggots. a vaccine against the major bacterial species found in fleece rot lesions, pseudomonas aeruginosa, is undergoing field trials and results suggest that this vaccine may reduce fleece rot incidence. problems to be investigated include the existence of variants of p. aeruginosa in the field and the involvement of other species of bacteria in fleece ... | 1990 | 2210946 |
| variation in skin inflammatory responses between sheep bred for resistance or susceptibility to fleece rot and blowfly strike. | sheep which have been bred for resistance (r) or susceptibility (s) to fleece rot and blowfly strike, were tested for intradermal inflammatory responses to excretory and secretory products of lucilia cuprina larvae. r rams and lambs gave significantly larger skin weals than s animals. in addition, r and s rams were infected with l cuprina first instar larvae and wound exudates were collected. in the first 12 hours of infection r rams released significantly more exudate protein at the wound site ... | 1992 | 1585078 |
| retarded growth of lucilia cuprina larvae on sheep and their sera following production of an immune response. | attempts were made to immunize sheep against larvae of the sheep blowfly lucilia cuprina using supernatant and pellet prepared by centrifuging (100,000 g max) homogenates from whole second instar larvae of l. cuprina or their excised guts. injection of supernatant from whole larvae and from two fractions of this supernatant, prepared by ammonium sulphate precipitation, significantly reduced (by 24-58%) the final weight of larvae grown in vivo (i.e. on immunized sheep) for 20 or 44 h. serum from ... | 1992 | 1587682 |
| ultrastructural localization of phenoloxidases in cuticle and haemopoietic tissue of the blowfly lucilia cuprina. | the ultrastructural localization of two types of biochemically characterized phenol oxidase activity is described in the larva of the sheep blowfly, lucilia cuprina. cuticular tyrosinase activity (enzyme a) is seen in epicuticular filaments and procuticle. procuticle activity can be detected only after a presumed process of activation takes place in damaged cuticle. by using either the dopamine reaction or inducing melanization by hot-water treatment, tyrosinase is readily shown in haemopoietic ... | 1988 | 3148210 |
| prevention of blowfly strike on sheep by cypermethrin. | merino ewe lambs (n = 500) were used in a trial to assess the efficacy of cypermethrin in preventing blowfly strike on sheep. three hundred of these lambs were treated with 300 mg cypermethrin each and run as a separate flock. one hundred lambs were used as untreated controls and a further 100 lambs were treated topically with a larval growth regulator as treated control animals. it was found that the synthetic pyrethroid afforded total protection for 3 weeks against strike by lucilia cuprina. t ... | 1988 | 3210218 |
| nucleolar fragmentation in polytene trichogen cells of lucilla cuprina and chrysomya bezziana (diptera: calliphoridae). | the location of genes coding for 18s and 28s ribosomal rna in mitotic and polytene cells of lucilia cuprina and chrysomya bezziana was investigated using in situ hybridization of an 18 + 28s ribosomal gene probe and silver staining. in both species ribosomal genes were localized to secondary constriction regions in sex chromosome heterochromatin. in l. cuprina mitotic cells the probe hybridizes to a distal secondary constriction region in the short arms of the x and y chromosomes. in c. bezziana ... | 1992 | 1618388 |
| in vitro and in vivo studies on the effects of flystrike dressings on larvae of lucilia cuprina. | | 1990 | 2264809 |
| shift of edge-taxis to scototaxis depends on mean luminance and is predicted by a matched filter theory on the responses of fly lamina lmc cells. | the strength of the flanking inhibitory regions of the receptive fields of fly lamina cells (lmc) decreases as the mean luminance is lowered. simultaneously, the biphasic temporal flash (impulse) response of the lamina cells becomes monophasic on lowering luminance. for a moving-edge stimulus at high mean luminance, this implies that the spatial integration by the lamina cell yields a temporal waveform which is congruent to the waveform of the temporal impulse response of the lamina cell. in oth ... | 1990 | 2278935 |
| the role of ph in the infestation of sheep by lucilia cuprina larvae. | | 1988 | 3397221 |
| a method of measuring the oviposition rate of the sheep blowfly, lucilia cuprina under field conditions. | a simple technique, which allows sheep to be rendered temporarily susceptible to fly attack, is described and used to examine oviposition rates of lucilia cuprina under field conditions. oviposition rates are shown to be related to both fly activity and fly density. the question of hobson's 'live sheep factor' in attracting l. cuprina to sheep is re-considered. | 1988 | 3401147 |
| potent immunosuppression by secretory/excretory products of larvae from the sheep blowfly lucilia cuprina. | secretory/excretory products (sec/ex) of parasitic larvae of the sheep blowfly lucilia cuprina potently inhibited proliferation of peripheral blood leucocytes stimulated by mitogens in vitro. suppression of proliferation was not due to irreversible damage because cells cultured for 24 h in high concentrations of sec/ex appeared viable (assessed by trypan blue exclusion) and did not show impaired proliferation after washing. furthermore, suppression induced by sec/ex could be overcome by increasi ... | 1992 | 1470479 |
| learning and discrimination of coloured papers in the walking blowfly, lucilia cuprina. | a new training and testing paradigm for walking sheep blowflies, lucilia cuprina, is described. a fly is trained by presenting it with a droplet of sugar solution on a patch of coloured paper. after having consumed the sugar droplet, the fly starts a systematic search. while searching, it is confronted with an array of colour marks consisting of four colours displayed on the test cardboard (fig. 1). colours used for training and test include blue, green, yellow, orange, red, white and black. bef ... | 1989 | 2600885 |
| immunization of sheep against infection with larvae of the blowfly lucilia cuprina. | | 1987 | 3570640 |
| the humoral immune response of sheep to antigens from larvae of the sheep blowfly (lucilia cuprina). | | 1987 | 3654049 |
| acquired resistance in sheep to infection with larvae of the blowfly, lucilia cuprina. | | 1986 | 3699979 |
| insecticidal control of lucilia cuprina: strategic timing of treatment. | | 1990 | 2288543 |
| flies (diptera) infesting landed fresh water fishes of the kainji lake area, nigeria. | eleven species of flies were found to land indiscriminately on fresh fish in fish monger's sheds. they include musca domestica, m. lusoria, fannia canicularis, stomoxys calcitran, lyperosia minuta, chrysomia chloropyga, c. bezziana, gastrophilus ovis, tabanus sp, lucilia cuprina and sacrophaga haemorrhoidalis. the public health significance of promiscuous landing of flies on fresh fish in relation to disease and fish spoilage are discussed. | 1986 | 3759356 |
| visual learning in walking blowflies, lucilia cuprina. | the australian sheep blowflies lucilia cuprina were trained by presenting droplets of sugar solution on a light spot of blue (460 nm wavelength) or green (520 nm wavelength). during the test, the searching behaviour was elicited by sugar stimulation. then, the flies were allowed to walk in the arena where four coloured spots (two blue and two green) with light intensities similar to the training light were exhibited. visits at these coloured spots were recorded. the flies visited preferably the ... | 1985 | 3837113 |
| what causes edge fixation in walking flies? | the orientation of freely walking flies (female lucilia cuprina) to lines and stripes in a circular arena is described. the following observations were made. 1. the flies walked straight towards a dark line using the frontal eye region, but a pale line on a dark background was only weakly attractive. 2. in bright conditions flies walked in a curved line towards a black-white edge, the path being convex towards the dark side of the border. the curves indicated that the flies were heading for a po ... | 1990 | 2324671 |
| male crossing over and genetic sexing systems in the australian sheep blowfly lucilia cuprina. | field-female killing (fk) systems based on deleterious mutations and y-autosome translocations are being evaluated for genetic control of the australian sheep blowfly, lucilia cuprina. experience during field trials has shown that mass-reared colonies of fk strains are subject to genetic deterioration, caused mainly by genetic recombination in males. a previous study found higher male recombination frequencies in two y-linked translocation strains than in chromosomally normal males. however, the ... | 1991 | 1774192 |
| initial characterisation of the sheep immune response to infections of lucilia cuprina. | | 1985 | 3997350 |
| the effect of inhibitors in vivo on protein synthesis and the amino acid pool in the sheep blowfly, lucilia cuprina. | 1. the rates of detoxification of cycloheximide (33 mug/g fresh wt.), puromycin (167 mug/g fresh wt.) and actinomycin d (1 mug/g fresh wt.) were assessed in vivo on the basis of acid-insoluble [14c]leucine incorporation in the sheep blowfly, lucilla cuprina; these were compared with quantitative estimates which took account not only of incorporation data but also of leucine pool size and turnover. quantitatively, cycloheximide and puromycin were still at least 50% effective in inhibiting protein ... | 1975 | 1180920 |
| inhalant allergy following occupational exposure to blowflies. | an allergic reaction, provoked by exposure to the blowfly lucilia cuprina and shown to be ige-mediated, occurred in a subject employed in an entomological research laboratory. the subject's serum, and sera from three other asthmatic patients with ige antibodies to blowfly extracts, also reacted with extracts from the screw-worm fly (chrysomya bezziana). results suggested that antigens from the two species share immunological cross-reactivity. cross-reactions also exist between the different deve ... | 1986 | 2420484 |
| effect of myiasis and acute restraint stress on plasma levels of immunoreactive beta-endorphin, adrenocorticotrophin (acth) and cortisol in the sheep. | cutaneous myiasis in sheep arising from the activity of lucilia cuprina larvae can result in significant physiological changes in susceptible animals. the stress imposed on the pituitary-adrenal axis of the sheep in response to myiasis and acute restraint is the subject of this investigation. merino wethers were exposed to handling restraint, and blood sampling, during examination for blowfly strike; where necessary, they were treated for cutaneous myiasis. significant changes in the plasma conc ... | 1988 | 2855918 |
| studies of the levels and synthesis of cytochrome c during adult development of the blowfly lucilia cuprina. | | 1972 | 4346800 |
| simulation of fly-waves to assess the ability of diflubenzuron to protect sheep against flystrike by lucilia cuprina. | sheep were exposed to mass-released gravid females of the sheep blowfly, lucilia cuprina, in a fly-proof animal house at various times after treatment with the insecticide diflubenzuron (1000, 1500 and 2500 ppm a.i.). untreated sheep were similarly exposed as controls, while sheep treated with either diazinon (400 ppm a.i.) or cyromazine (1000 ppm) were used as standards for comparison. before exposure, groups of sheep were wetted by simulating rainfall in the animal house in order to increase t ... | 1987 | 3113043 |
| reduction in period of protection from artificial flystrike by organophosphorus and organophosphorus-carbamate resistant larvae of lucilia cuprina. | | 1974 | 4471314 |
| purified glutathione s-transferases from parasites as candidate protective antigens. | glutathione s-transferase (gst) activity was detected in larvae of the australian sheep blowfly lucilia cuprina, and in the nematode haemonchus contortus. a specific inhibitor of the enzyme was shown to affect survival of both species of parasite in vitro. gst from both parasites has been purified and partially characterized. antisera raised to the purified enzymes were shown to inhibit the enzyme activity in vitro. however, the antisera had no effect on the survival of either parasite. | 1991 | 1774119 |
| a significant increase in larval resistance of lucilia cuprina wied. to diazinon. | | 1974 | 4850295 |
| gold labeling with wheat germ agglutinin and rnase on osmicated tissue embedded in epoxy resin. | tissue of an insect, lucilia cuprina, fixed conventionally in buffered glutaraldehyde and osmium and embedded in epoxy resin (epon or epon/araldite), provided sections which could readily be labeled with rnase/gold and wheat germ agglutinin (wga)/gold. this method offers labeling of tissues with improved contrast and allows the retrospective application of rnase and wga labeling to conventionally prepared tissues, without recourse to oxidizing/etching agents. | 1989 | 2476480 |
| incorporation in vitro of ( 14 c)leucine into the mitochondrial protein of lucilia cuprina. 1. basic requirements. | | 1971 | 4999815 |
| polytene chromosomes of the old world screwworm fly (chrysomya bezziana) and its evolutionary relationships with lucilia cuprina and cochliomyia hominivorax (diptera: calliphoridae). | standard polytene chromosome maps for the old world screwsworm fly, chrysomya bezziana, are presented. good quality polytene chromosomes obtainable from pupal trichogen cells allow detailed analysis of autosomal euchromatin. the sex chromosomes are represented by irregular heterochromatic structures resembling those described previously in trichogen polytene chromosomes of the australian sheep blowfly, lucilia cuprina. a high degree of homology with the banding pattern of l. cuprina polytene chr ... | 1992 | 1618389 |
| incorporation in vitro of ( 14 c)leucine into the mitochondrial protein of lucilia cuprina. 2. energy requirements. | | 1971 | 5099220 |
| lucilia cuprina induced hyperammonaemia and alkalosis associated with pathology in sheep. | gross and microscopical examinations were done in 12 sheep infested with lucilia cuprina larvae. all the sheep became hyperammonaemic and alkalotic. six sheep died and 6 survived, one being moribund before euthanasia. necropsy revealed severe acute dermatitis and congestion of the liver, heart, kidney, spleen and lungs. ecchymotic haemorrhages were present in the heart, kidneys and adrenal glands of severely infested sheep. microscopical examination showed mild to severe vacuolation of the centr ... | 1988 | 3392704 |
| sexual stimulation of males of lucilia cuprina (calliphoridae) and drosophila melanogaster (drosophilidae) by the odors of aggregation sites. | | 1969 | 5374179 |
| ultrastructure of the peritrophic membrane-secreting cells in the cardia of the blowfly, lucilia cuprina. | scanning and transmission electron microscopy are used to reveal the internal anatomy and ultrastructure of the cardia which is the source of the triple layered peritrophic membrane in the blowfly lucilia cuprina. within the cardia, rings of secretory cells (formation zones) and non-secretory tissue (valvula cardiaca) interlock to secrete and mould the layers of membrane. formation zone cells have abundant rough endoplasmic reticulum, golgi and secretory vesicles. a portion of midgut just poster ... | 1988 | 3406943 |
| a resistance mechanism in organophosphorus-resistant strains of sheep blowfly (lucilia cuprina). | | 1968 | 5643263 |
| seasonal abundance of carrion-frequenting blow-flies (diptera: calliphoridae) in the kruger national park. | monthly population fluctuations of carrion-frequenting blow-flies over a 24-month period were monitored using 2 carrion-baited traps in the southern kruger national park (knp) and 3 in the northern knp. all species displayed a clear seasonality. chrysomyia marginalis and chrysomyia albiceps were by far the most abundant. c. marginalis attained maximum abundance between november and march, with relatively low numbers present between may and september. c. albiceps maintained high population number ... | 1987 | 3444614 |
| development of respiratory activity and oxidative phosphorylation in flight muscle mitochondria of the blowfly, lucilia cuprina. | | 1969 | 5767703 |
| plasma leakage in skin of sheep selected for resistance or susceptibility to fleece rot and fly strike. | the leakage of plasma into skin following injection of histamine, bradykinin, activated complement, platelet-activating factor and serotonin was measured in sheep bred for resistance or susceptibility to fleece rot and fly strike. genetically susceptible sheep had significantly greater plasma leakage to activated complement than genetically resistant sheep, and for all mediators there was a trend for plasma leakage to be greater in susceptible sheep. within each genotype there was also a tendenc ... | 1992 | 1470478 |
| characterization of proteolytic and collagenolytic enzymes from the larvae of lucilia cuprina, the sheep blowfly. | isoelectric focusing was used to characterize proteolytic enzymes in homogenate and excretory-secretory preparations of the larvae of l. cuprina, the sheep blowfly. zymogram overlays showed that the larvae produce a number of highly active proteases which have a wide range of isoelectric points and molecular weights. the alkaline and neutral pi proteases were inhibited by phenylmethyl-sulfonylfluoride, leupeptin and aprotinin; this indicated the presence of serine in the active site. pepstatin a ... | 1988 | 2480778 |
| heterorhabditis spp. and steinernema (= neoaplectana) spp.: temperature, and aspects of behavior and infectivity. | the infectivity of several species/strains of nematodes of the genera heterorhabditis and steinernema for postfeeding, 3rd instar larvae of the sheep blowfly, lucilia cuprina, was tested in sand at various temperatures. of the two genera, the steinernematids were more active at lower temperatures and parasitized l. cuprina over a greater temperature range. the temperature range of infectivity for l. cuprina differed between nematodes of the same genus and between strains of the same species. par ... | 1986 | 3743713 |
| human cutaneous myiasis in brisbane: a prospective study. | although cases of human cutaneous myiasis do occur in queensland, few have been reported in the medical literature. no prospective studies to record and to identify the diptera that are responsible in a particular area seem to have been reported. thus, a prospective study was undertaken in brisbane hospitals between october 9, 1986 and march 15, 1988. fourteen infestations were recorded and, in 12 cases, the larvae were bred through to adult flies. in 10 cases, lucilia cuprina (wiedemann) was id ... | 1989 | 2716619 |
| ecological analysis of field trials conducted to assess the potential of sex-linked translocation strains for genetic control of the australian sheep blowfly, lucilia cuprina (wiedemann). | field trials were conducted with translocation/eye colour (te) strains of l. cuprina to measure the mating ability of the males under field conditions and assess their potential for suppressing sheep blowfly populations. rates of increase in l. cuprina were highest in spring (3.9-9.1 per generation), consistently low during summer (0.1-0.6 per generation) and somewhat higher during autumn (1.1-3.4 per generation). the te strains released had the potential to prevent population increases of this ... | 1985 | 4091754 |
| specific resistance to malathion in laboratory and field populations of the australian sheep blowfly, lucilia cuprina (diptera: calliphoridae). | | 1984 | 6520294 |
| occupational allergy in an entomological research centre. ii. identification of ige-binding proteins from developmental stages of the blowfly lucilia cuprina and other species of adult flies. | sera from 30 workers in an entomological research institute and from five sydney asthmatics, all with serum ige antibodies to sheep blowfly (lucilia cuprina) antigens, were studied with the aim of identifying individual ige-binding proteins in extracts of l. cuprina adult flies, larvae and eggs, and in extracts of related species of flies from the order diptera. using protein blotting, 21, 18 and nine different ige-binding components were identified in extracts of l. cuprina adult flies, larvae ... | 1989 | 2758354 |
| genetic analysis of field trials of sex-linked translocation strains for genetic control of the australian sheep blowfly lucilia cuprina (wiedemann). | the results of progeny tests of males and females captured during two field trials of sex-linked translocation strains for genetic control of l. cuprina are presented. males released as mature larvae survived to adulthood and mated with field females. however, the levels of genetic death introduced into the population were insufficient to suppress the native population. this was due partly to seasonal ineffectiveness of the release method, and partly to poor performance of the released males. on ... | 1985 | 4091755 |
| new techniques for measuring the growth and survival of larvae of lucilia cuprina on sheep. | | 1989 | 2775057 |
| the distribution of nicotinamide nucleotides during the life cycle of the blowfly lucilia cuprina wied. | 1. variations in the amounts and distribution of the nicotinamide coenzymes during the life cycle of the blowfly lucilia cuprina have been investigated. 2. the concentrations of nad and nadp declined to a minimum in mid-pupal life, then increased threefold from this to about 600 and 40mmumoles/g. fresh wt. respectively in the adult. 3. the nadh/nad ratio was relatively constant (0.15) except in mid-pupal life (0.09); the nadph/nadp ratio was lower (0.6) in larvae and mated adults than at other s ... | 1966 | 4381709 |
| the intracellular pupil mechanism and photoreceptor signal: noise ratios in the fly lucilia cuprina. | the function of the intracellular pupil mechanism is examined by comparing the responses of photoreceptors in normal flies with those from white-eyed flies that lack the pupil. in white-eyed flies the response to an intensity increment of fixed contrast decreases at high background intensities. there is a smaller decrease in noise amplitude so that the signal:noise ratio falls. the intensity dependence of the photoreceptor signal:noise ratio fits a simple model in which activated photopigment mo ... | 1987 | 2892201 |
| susceptibility of larvae of the sheep blowfly lucilia cuprina to various heterorhabditis spp., neoaplectana spp., and in undescribed steinernematid (nematoda). | | 1983 | 6886466 |
| the role of some cyclorrhaphan flies as carriers of human helminths in malaysia. | the role of some adult flies (diptera: cyclorrhapha) as carriers of helminth parasites of man was studied at four sites in malaysia: a refuse dump, where no helminth-positive flies were detected, and in three peri-domestic situations where four species of flies carried up to three types of nematodes. the dominant fly species chrysomya megacephala (fabricius) carried eggs of the roundworm ascaris lumbricoides l., the pinworm trichuris trichiura (l.) and hookworm on the adult external body surface ... | 1988 | 2980156 |