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the occurrence of the transposable element pogo in drosophila melanogaster.we examined the genomic occurrence of the transposable element pogo in over 120 strains of drosophila melanogaster, from around the world and from different eras. all had multiple copies of a 2.1 kilobase (kb) pogo element, and multiple copies of several size classes between 1.0 and 1.8 kb. there were differences between strains in intensities or presences of deletion-derivative size classes, suggesting current or recent mobility in the species. we were unable to find any pogo-hybridization in e ...19938397138
transcription and splicing patterns of m- and o-type p elements in drosophila bifasciata, d. helvetica, and scaptomyza pallidart-pcr was applied to analyze the splicing patterns of p-element-derived mrnas in drosophila bifasciata, d. helvetica, and scaptomyza pallida. d. melanogaster was used as a control. the experiments revealed that p elements are transcribed in all species investigated. however, there are differences in the splicing patterns of ivs3, which has to be removed in order to produce transposase mrna instead of repressor mrna. these differences are observed among species as well as between the p element s ...19989545465
repeated horizontal transfer of p transposons between scaptomyza pallida and drosophila bifasciata.two distinct p element subfamilies, designated m-type and o-type, reside in the genome of d. bifasciata. pcr-screening of 65 drosophila species revealed that only d. bifasciata and its closest relative d. imaii possess o-type elements. outside the genus, o-type elements were detected in scaptomyza pallida. restriction analyses show that the general structure of the o-type elements from s. pallida and d. bifasciata is the same. sequence divergence turned out to be extremely low (0.43%). these res ...19968765681
two distinct p element subfamilies in the genome of drosophila bifasciata.the genome of drosophila bifasciata harbours two distinct subfamilies of p-homologous sequences, designated m-type and o-type elements based on similarities to p element sequences from other species. both subfamilies have some general features in common: they are of similar length (m-type: 2935 bp, o-type: 2986 bp), are flanked by direct repeats of 8 bp (the presumptive target sequence), contain terminal inverted repeats, and have a coding region consisting of four exons. the splice sites are at ...19948052236
identification of a complete p-element in the genome of drosophila bifasciata.a full-size p-element (ibifm3) was isolated from a genomic library of drosophila bifasciata. the sequence has a length of 2935 bp and is flanked by 8 bp duplications of the target site. the termini are formed by 31 bp inverted repeats. the four exons have intact reading frames and possess the coding capacity for a protein of 753 amino acids and a molecular weight of 86.4 kd. the sections of the d. melanogaster transposase presumed to be functionally important (three leucine zippers and a helix t ...19921311074
a p element of scaptomyza pallida is active in drosophila melanogaster.several results suggest that p elements have recently invaded natural populations of drosophila melanogaster after a horizontal transfer from another species. the donor species is thought to come from the willistoni group, which contains p elements very homologous to those of d. melanogaster. however, more divergent p elements are present in many other drosophilidae species. we have analyzed such elements from scaptomyza pallida, a species phylogenetically distant to d. melanogaster. we report h ...19911648729
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