| chromosome variability in brazilian populations of drosophila buzzatii (diptera, drosophilidae). | analysis of 28 strains of drosophila buzzatii from six different localities revealed that populations from southern brazil apparently differ from northeastern populations in chromosome structure. of the 16 polymorphic chromosome inversions detected in this species, only two (2j and 5c2) were present in southern populations. the northeastern populations were the first of this species found not to have the 2j inversion. this suggests that the populations of drosophila buzzatii may have lost chromo ... | 1992 | 1340927 |
| genetic variance for body size in a natural population of drosophila buzzatii. | previous work has shown thorax length to be under directional selection in the drosophila buzzatii population of carboneras. in order to predict the genetic consequences of natural selection, genetic variation for this trait was investigated in two ways. first, narrow sense heritability was estimated in the laboratory f2 generation of a sample of wild flies by means of the offspring-parent regression. a relatively high value, 0.59, was obtained. because the phenotypic variance of wild flies was ... | 1991 | 1916242 |
| breeding structure of natural populations of drosophila buzzatii: effects of the distribution of larval substrates. | the population structure of several australian populations of the cactophilic drosophila buzzatti was investigated, with seasonal samples from two populations analyzed for six polymorphic allozymes. significant inbreeding throughout the year was detected in both populations, and significant but relatively slight differences in allele frequencies between collections were found in one population. little significant 2-locus linkage disequilibrium was detected and the variances of linkage disequilib ... | 1990 | 2358367 |
| polymorphic inversion and esterase loci complex on chromosome 2 of drosophila buzzatii. ii. spatial variation. | the potential influence of linked inversions on allele frequency variation at the est-1 and est-2 loci among australian populations of d. buzzatii was determined by statistical analyses allele and inversion gametic frequencies. most of the significant spatial and climatic associations found for all est-1 allele frequencies, and for one allele only of est-2 (est-2c+), were accounted for by their linkage disequilibria with the inversions, which covaried with environmental variables. consistent wit ... | 1988 | 3270311 |
| differential response to environmental alcohol among second-chromosome arrangements in experimental populations of drosophila buzzatii. | drosophila buzzatii feeds and breeds on the decaying cladodes and fruits of several species of opuntia (prickly pear) which contain significant levels of ethanol and isopropanol. the potential influence of these two alcohols on the inversion polymorphism of the second and fourth chromosomes was investigated in ten experimental populations with different amounts of alcohol (either ethanol or isopropanol) added to the culture medium. all populations were started with the offspring of 29 wild femal ... | 1987 | 3505227 |
| temporal and microgeographic variation in allozyme frequencies in a natural population of drosophila buzzatii. | temporal variation in allozyme frequencies at six loci was studied by making monthly collections over 4 yr in one population of the cactophilic species drosophila buzzatii. ten sites were defined within the study locality, and for all temporal samples, separate collections were made at each of these sites. population structure over microgeographic space and changes in population structure over time were analyzed using f-statistic estimators, and multivariate analyses of allele and genotype frequ ... | 1986 | 3957005 |
| genetic structure is determined by stochastic factors in a natural population of drosophila buzzatii in argentina. | d. buzzatii is a cactophilic species associated with several cactaceae in argentina. this particular ecological niche implies that this species is faced with a non-uniform environment constituted by discrete and ephemeral breeding sites, which are colonized by a finite number of inseminated females. the genetic consequences of this population structure upon the second chromosome polymorphism were investigated by means of f-statistics in a natural endemic population of argentina. the present stud ... | 1994 | 7958936 |
| sequential gel electrophoretic analysis of esterase-2 in two populations of drosophila buzzatii. | sequential electrophoresis, using three different buffer systems on cellulose acetate gels, was used to characterize the allelic variation for esterase-2 in two populations of d. buzzatii in australia that are separated by 550 km. twenty-five alleles were detected, of which nine were unique to one population, eight unique to the other, and only eight were common to both populations. allele frequencies within each population were significantly different between the two major chromosome sequences ... | 1994 | 7958940 |
| response to environmental change: genetic variation and fitness in drosophila buzzatii following temperature stress. | drosophila buzzatii typically may encounter high temperatures in nature, and this species is genetically variable for resistance to stress, both within and among populations. fitness of survivors to stress, however, was reduced, and observed as a reduction in male fertility and female fecundity. with time following exposure to severe stress, reproductive capacity improved, but lifetime offspring production still was reduced significantly. this effect would greatly reduce a population's recovery ... | 1994 | 8032136 |
| the evolutionary history of drosophila buzzatii. xxvii. thorax length is positively correlated with longevity in a natural population from argentina. | the correlation between body size and longevity was tested in an argentinian natural population of drosophila buzzatii. mean thorax length of flies newly emerging from rotting cladodes of opuntia vulgaris was significantly smaller than that of two samples of flies caught at baits. the present results which might be interpreted as directional selection for longevity favoring larger flies are in agreement with previous results achieved in a spanish natural population of d. buzzatii. flies emerging ... | 1993 | 8163157 |
| genotype-specific habitat selection for oviposition sites in the cactophilic species drosophila buzzatii. | isofemale lines of the cactophilic species, drosophila buzzatii, exhibit genetic variation for their oviposition response to cactus yeast species in the laboratory. in general, interactions between yeast species preclude the use of pairwise preferences as predictors of preferences in three-way choice experiments. two isofemale lines with relatively high laboratory preference for ovipositing on the yeast pichia cactophila (as opposed to cryptococcus cereanus) and two isofemale lines with relative ... | 1994 | 8200815 |
| evolutionary cytogenetics of the drosophila buzzatii species complex. | the salivary gland chromosomes of 10 species in the drosophila mulleri subgroup (repleta group) have been re-analysed. these include the eight members of the south american buzzatti and martensis clusters, previously ascribed to the mulleri complex, and the two caribbean species d. stalkeri and d. richardsoni, previously comprising the stalkeri complex. the chief results can be summarized as follows. inversion 3a is not present in the martensis cluster. hence, there is no cytological link betwee ... | 1993 | 8335477 |
| the evolutionary history of drosophila buzzatii. xxx. mitochondrial dna polymorphism in original and colonizing populations. | both original and colonizer populations of drosophila buzzatii have been analyzed for mtdna restriction polymorphisms. most of the mtdna nucleotide variation in original populations of nw argentina can be explained by intrapopulation diversity and only a small fraction can be accounted for by between-population diversity. similar results are obtained using either the estimated number of nucleotide substitutions per site or considering each restriction site as a locus. colonizer populations of th ... | 1996 | 8587497 |
| the evolutionary history of drosophila buzzatii. xxxiii. are opuntia hosts a selective factor for the inversion polymorphism? | previous work has shown fitness differences among chromosomal arrangements by means of selection component analysis in two drosophila buzzatii natural populations, one of which is native to argentina and the other a colonized population from carboneras, spain. founder effects or niche shifts were proposed to explain the differences observed in the pattern of pleiotropic effects of inversions on fitness components. in this paper, we address the possible role of niche shifts by determining whether ... | 1996 | 8939019 |
| the effect of polymorphic inversions on body size in two natural populations of drosophila buzzatii from argentina. | previous works in a colonized and an original population of drosophila buzzatii have shown a consistent relationship between the inversion polymorphism and thorax length, a measure of body size. however, the populations studied in those reports share a close genealogical relationship as suggested by several lines of evidence. in the present paper, we revisit this issue by analysing the correlation between second chromosome arrangements and thorax length in two argentinian natural populations (te ... | 1997 | 9350138 |
| environmental effects and the genetics of oviposition site preference for natural yeast substrates in drosophila buzzatii | habitat selection expressed as oviposition site preferences (osp), is one component of the complex of behaviours of females seeking a place to oviposit. drosophila buzzatii females lay their eggs in cactus necroses (rots), where the alternative oviposition sites are patches of adjacent or even partially mixed growing yeast species. the osp exhibited by individual females is not absolute, but subject to environmental effects and the physiology of the fly, and may vary depending on the particular ... | 1999 | 10479998 |
| the role of the use of different host plants in the maintenance of the inversion polymorphism in the cactophilic drosophila buzzatii. | inversion polymorphisms often have been associated with fitness variation. cactophilic drosophila buzzatii has been used widely for the study of the maintenance of chromosomal variation. the purpose of this paper is to address the relative importance of variable selection regimes associated with the use of three different host cacti and antagonistic pleiotropy in the maintenance of chromosomal variation. using homokaryotypic stocks derived from several lines homozygous for four second-chromosome ... | 2000 | 11005296 |
| behavioral differentiation in oviposition activity in drosophila buzzatii from highland and lowland populations in argentina: plasticity or thermal adaptation? | highland populations of several drosophila species in argentina were active early in the afternoon in the field as opposed to populations from a much warmer lowland site, where flies were mainly active in the early evening prior to sunset. for one of these species, drosophila buzzatii, we tested for a genetic component of activity differences by carrying out crosses within and between populations and measuring oviposition activity of the progeny in the laboratory. we found that activity in the h ... | 2001 | 11392392 |
| chromosomal distribution of the transposable elements osvaldo and blanco in original and colonizer populations of drosophila buzzatii. | chromosomal distribution of transposable elements (tes) osvaldo and blanco in d. buzzatii was studied in three original natural populations from argentina (berna, puerto tirol and la nostalgia) and a colonizer population from the iberian peninsula (carboneras). the spanish population showed significant differences for osvaldo and blanco copy numbers when we compared the x chromosome and the autosomes; but it is mainly the accumulation of copies in chromosome 2, where most sites with high inserti ... | 2001 | 11486506 |
| gametic associations between inversion and allozyme polymorphisms in drosophila buzzatii. | gametic disequilibria between second chromosome polymorphic arrangements and seven linked allozyme loci were estimated in seven populations of drosophila buzzatii from argentina. significant and consistent associations across populations were detected for est-1, est-2, aldox, and xdh: phenograms based on nei's genetic distance showed that chromosomes carrying the 2st arrangement were more similar to each other, irrespective of the population from which they were extracted, than to chromosomes ca ... | 2001 | 11773244 |
| developmental time, body size and wing loading in drosophila buzzatii from lowland and highland populations in argentina. | genotype-by-temperature interaction is a necessary condition for adaptive evolution of fitness traits as a response to temperature. several fitness-related traits (developmental time, pre-adult survival, thorax and wing lengths, and wing loading) were measured in laboratory-reared d. buzzatii from four populations sampled at different altitudes in north-western argentina: a lowland population (407 m a.s.l.), two populations from intermediate altitude (780 to 950 m a.s.l.), and a highland populat ... | 2001 | 12035612 |
| comparative molecular population genetics of the xdh locus in the cactophilic sibling species drosophila buzzatii and d. koepferae. | the xdh (rosy) gene is one of the best studied in the drosophila genus from an evolutionary viewpoint. here we analyze nucleotide variation in a 1875-bp fragment of the second exon of xdh in argentinian populations of the cactophilic d. buzzatii and its sibling d. koepferae. the major electrophoretic alleles of d. buzzatii not only lack diagnostic amino acids in the region studied but also differ on average from each other by four to 13 amino acid changes. our data also suggest that d. buzzatii ... | 2004 | 14595098 |
| altitudinal variation for stress resistance traits and thermal adaptation in adult drosophila buzzatii from the new world. | multiple stress resistance traits were investigated in the cactophilic fly drosophila buzzatii. adults from seven populations derived from north-western argentina were compared with respect to traits relevant for thermal stress resistance and for resistance to other forms of environmental stress. the populations were collected along an altitudinal gradient spanning more than 2000 m in height, showing large climatic differences. the results suggest that knock-down resistance to heat stress, desic ... | 2005 | 16033554 |
| climatic adaptation of drosophila buzzatii populations in southeast australia. | variation in 19 traits possibly relevant for thermal adaptation was studied in 11 populations of drosophila buzzatii collected in southeast australia. using stepwise multiple regression, the variation was compared to variation in geographic coordinates and to a set of climatic variables estimated for each collection site. for 13 of the traits, a significant part of the variation was explained by climatic variables and/or geographic coordinates, suggesting directional selection for adaptation to ... | 2006 | 16622471 |
| genotype by environment interactions in viability and developmental time in populations of cactophilic drosophila. | the genetic and ecological basis of viability and developmental time differences between drosophila buzzatii and d. koepferae were analysed using the isofemale line technique. several isofemale lines were sampled from pairs of allopatric/sympatric populations of each species. flies were reared in media prepared with decaying tissues of two of the main natural cactus hosts of each species. this experimental design enabled us to evaluate the relative contribution of phenotypic plasticity, genetic ... | 2006 | 16674586 |
| aedeagus morphology as a discriminant marker in two closely related cactophilic species of drosophila (diptera; drosophilidae) in south america. | drosophila serido and d. antonietae are sibling species belonging to the drosophila buzzatii cluster. morphologically, they can only be discriminated by quantitative traits. in this paper we analyze the length and equalized average curvature of four regions of the aedeagus of d. antonietae and d. serido. specimens of d. serido and d. antonietae were classified correctly 96.74% of the time. based only on the variable that most contributed to the discrimination of the groups (equalized average cur ... | 2006 | 16710560 |
| altitudinal patterns for longevity, fecundity and senescence in drosophila buzzatii. | we tested for variation in longevity, senescence rate and early fecundity of drosophila buzzatii along an elevational transect in argentina, using laboratory-reared flies in laboratory tests performed to avoid extrinsic mortality. at 25 degrees c, females from lowland populations lived longer and had a lower demographic rate of senescence than females from highland populations. minimal instead of maximal temperature at the sites of origin of population best predicted this cline. a very different ... | 2006 | 17028942 |
| conservation of pbum-2 satellite dna sequences among geographically isolated drosophila gouveai populations from brazil. | in this study, we have compared 34 repetition units of pbum-2 satellite dna of individuals from six isolated populations of drosophila gouveai, a cactophilic member of drosophila buzzatii cluster (repleta group). in contrast to the results of previous morphological and molecular data, which suggest differentiation among the d. gouveai populations, the sequences and the cluster analysis of pbum-2 monomers showed that this repetitive element is highly conserved among the six d. gouveai populations ... | 2006 | 17028958 |
| developmental time and size-related traits in drosophila buzzatii along an altitudinal gradient from argentina. | clinal analysis for fitness-related traits provides a well-known approach to investigate adaptive evolution. several fitness-related traits (developmental time, thorax length, wing length and wing loading) were measured at two laboratory generations (g7 and g33) of d. buzzatii from an altitudinal gradient from northwestern argentina, where significant thermal differences persist. developmental time (dt) was positively correlated with altitude of origin of population. further, dt was negatively c ... | 2006 | 17362338 |
| wing morphology and fluctuating asymmetry depend on the host plant in cactophilic drosophila. | as in most insect groups, host plant shifts in cactophilic drosophila represent environmental challenges as flies must adjust their developmental programme to the presence of different chemical compounds and/or to a microflora that may differ in the diversity and abundance of yeasts and bacteria. in this context, wing morphology provides an excellent opportunity to investigate the factors that may induce changes during development. in this work, we investigated phenotypic plasticity and developm ... | 2008 | 18081744 |
| bottlenecks, population differentiation and apparent selection at microsatellite loci in australian drosophila buzzatii. | species colonizing new areas disjunct from their original habitat may be subject to novel selection pressures, and exhibit adaptive genetic changes. however, if colonization occurs through a small number of founders, the genetic composition of the colonized population may differ from that of the original population simply due to genetic drift. disentangling the effects of founder drift and selection after colonization is crucial to understanding the adaptive process. drosophila buzzatii colonize ... | 2009 | 19142202 |
| characterization of the shsp genes in drosophila buzzatii and association between the frequency of valine mutations in hsp23 and climatic variables along a longitudinal gradient in australia. | the small heat shock gene (shsp) cluster of drosophila buzzatii was sequenced and the gene order and dna sequence were compared with those of the shsps in drosophila melanogaster. the d. buzzatii shsp cluster contains an inversion and a duplication of hsp26. a phylogenetic tree was constructed based on hsp26 genes from several drosophila species of the sophophora and drosophila subgenera. the tree shows first a separation of the sophophora and the drosophila subgenera and then the drosophila sub ... | 2010 | 19806471 |
| developmental acclimation affects clinal variation in stress resistance traits in drosophila buzzatii. | patterns of clinal genetic variation in drosophila are often characterized after rearing at constant temperatures. however, clinal patterns might change after acclimation if populations differ in their plastic response to fluctuating environments. we studied longevity, starvation and heat knock-down resistance after development at either constant or fluctuating temperatures in nine drosophila buzzatii populations collected along an altitudinal gradient in tenerife, spain. flies that developed at ... | 2010 | 20298441 |
| local adaptation of stress related traits in drosophila buzzatii and drosophila simulans in spite of high gene flow. | we addressed the question if local adaptation to a thermal gradient is possible in spite of a high gene flow among closely spaced populations of two species of drosophila from the island of la gomera (canary islands). variation in multiple traits related to stress resistance in different life stages was measured in both species in flies collected from five localities at different altitudes and thereby with different climatic conditions. based on microsatellite loci, the populations were not gene ... | 2009 | 21462416 |
| the genetics of abnormal abdomen, incomplete abdomen, and bobbed in drosophila buzzatii. | five stocks of drosophila buzzatii with superficially similar abdominal disruptions including partial tergite and sternite loss were isolated by inbreeding. three of the stocks have indistinguishable phenotypes, the inheritance of which is maternally influenced. this phenotype and its mode of inheritance bear similarities with those of abnormal abdomen in d. melanogaster. the phenotype in the fourth stock is slightly different and is due to a single autosomal recessive gene, which we denote inco ... | 2008 | 2620817 |
| but2 is a member of the third major group of hat transposons and is involved in horizontal transfer events in the genus drosophila. | the hat superfamily comprises a large and diverse array of dna transposons found in all supergroups of eukaryotes. here we characterized the drosophila buzzatii but2 element and found that it harbors a five-exon gene encoding a 643-aa putatively functional transposase. a phylogeny built with 85 hat transposases yielded, in addition to the two major groups already described, ac and buster, a third one comprising 20 sequences that includes but2, tip100, hat-4_bm, and rp-hat1. this third group is h ... | 0 | 24459285 |
| structural and sequence diversity of the transposon galileo in the drosophila willistoni genome. | galileo is one of three members of the p superfamily of dna transposons. it was originally discovered in drosophila buzzatii, in which three segregating chromosomal inversions were shown to have been generated by ectopic recombination between galileo copies. subsequently, galileo was identified in six of 12 sequenced drosophila genomes, indicating its widespread distribution within this genus. galileo is strikingly abundant in drosophila willistoni, a neotropical species that is highly polymorph ... | 2014 | 25218200 |
| molecular characterization of two natural hotspots in the drosophila buzzatii genome induced by transposon insertions. | transposable elements (tes) have been implicated in the generation of genetic rearrangements, but their potential to mediate changes in the organization and architecture of host genomes could be even greater than previously thought. here, we describe the naturally occurring structural and nucleotide variation around two te insertions in the genome of drosophila buzzatii. the studied regions correspond to the breakpoints of a widespread chromosomal inversion generated by ectopic recombination bet ... | 0 | 11483576 |
| the transposon galileo generates natural chromosomal inversions in drosophila by ectopic recombination. | transposable elements (tes) are responsible for the generation of chromosomal inversions in several groups of organisms. however, in drosophila and other dipterans, where inversions are abundant both as intraspecific polymorphisms and interspecific fixed differences, the evidence for a role of tes is scarce. previous work revealed that the transposon galileo was involved in the generation of two polymorphic inversions of drosophila buzzatii. | 2009 | 19936241 |
| silencing of a gene adjacent to the breakpoint of a widespread drosophila inversion by a transposon-induced antisense rna. | adaptive changes in nature occur by a variety of mechanisms, and drosophila chromosomal inversions was one of the first studied examples. however, the precise genetic causes of the adaptive value of inversions remain uncertain. here we investigate the impact of the widespread inversion 2j of drosophila buzzatii on the expression of the cg13617 gene, whose coding region is located only 12 bp away from the inversion proximal breakpoint. this gene is transcribed into a 2.3-kb mrna present in all d. ... | 2004 | 15184654 |
| conservation of regulatory sequences and gene expression patterns in the disintegrating drosophila hox gene complex. | homeotic (hox) genes are usually clustered and arranged in the same order as they are expressed along the anteroposterior body axis of metazoans. the mechanistic explanation for this colinearity has been elusive, and it may well be that a single and universal cause does not exist. the hox-gene complex (hom-c) has been rearranged differently in several drosophila species, producing a striking diversity of hox gene organizations. we investigated the genomic and functional consequences of the two h ... | 0 | 15867430 |
| first record of drosophila buzzatii (patterson & wheeler) (diptera: drosophilidae) emerging from a non-cactus host. | drosophila buzzatii (patterson & wheeler), a typical cactophilic species of the repleta group, is registered for the first time emerging from melon (cucumis melo) in western argentina. the analysis of inversion polymorphism and genetic diversity of mitochondrial cytochrome oxidase subunit i gene (mtcoi) provided additional evidence that corroborated the presence of a high proportion of d. buzzatii among the flies emerged from melon. this finding set the scenario for a broader range of possible h ... | 2016 | 26960546 |
| the foldback-like element galileo belongs to the p superfamily of dna transposons and is widespread within the drosophila genus. | galileo is the only transposable element (te) known to have generated natural chromosomal inversions in the genus drosophila. it was discovered in drosophila buzzatii and classified as a foldback-like element because of its long, internally repetitive, terminal inverted repeats (tirs) and lack of coding capacity. here, we characterized a seemingly complete copy of galileo from the d. buzzatii genome. it is 5,406 bp long, possesses 1,229-bp tirs, and encodes a 912-aa transposase similar to those ... | 2008 | 18287066 |
| abundance and chromosomal distribution of six drosophila buzzatii transposons: but1, but2, but3, but4, but5, and but6. | the abundance and chromosomal distribution of six class-ii transposable elements (tes) of drosophila buzzatii have been analyzed by southern blotting and in situ hybridization. these six transposons had been previously found at the breakpoints of inversions 2j and 2q ( 7 ) of d. buzzatii. these two polymorphic inversions were generated by an ectopic recombination event between two copies of galileo, a foldback element. the four breakpoints became hotspots for te insertions after the generation o ... | 2006 | 16773395 |
| the evolutionary history of drosophila buzzatii. xxxiv. the distribution of the retrotransposon osvaldo in original and colonizing populations. | the frequency distribution of the retrotransposon osvaldo in the haploid genome of drosophila buzzatii has been studied in five natural populations from the iberian peninsula and six natural populations from argentina. in iberian populations, osvaldo insertion sites do not follow a poisson distribution, most probably due to eight euchromatic sites with high occupancy, found in all populations. the estimated alpha and beta parameters, which measure the relative importance of drift and negative se ... | 1998 | 12572617 |
| duplication, dicistronic transcription, and subsequent evolution of the alcohol dehydrogenase and alcohol dehydrogenase-related genes in drosophila. | it has recently been discovered that the alcohol dehydrogenase and alcohol dehydrogenase-related genes of drosophila melanogaster and closely related species constitute a single transcription unit and that the alcohol dehydrogenase-related gene is exclusively expressed from a dicistronic mrna. here, we show that in drosophila lebanonensis, subgenus scaptodrosophila, adhr: is also transcribed as a dicistronic transcript with adh using degenerate primers designed on the sequence of the known adhr ... | 2000 | 10958851 |
| reconstructing the diversification of alpha-esterases: comparing the gene clusters of drosophila buzzatii and d. melanogaster. | a cluster composed of 10 active alpha-esterase genes and a pseudogene is distributed over 60 kb in the drosophila melanogaster genome. this paper describes the corresponding cluster in drosophila buzzatii, whose lineage diverged from that of d. melanogaster when the subgenera drosophila and sophophora diverged about 50 mya. with three exceptions we find that the composition of the cluster is conserved in the two lineages. the location of alpha e1 in d. melanogaster differs from that of its neare ... | 2000 | 10948271 |
| interspecific hybridization increases transposition rates of osvaldo. | several authors have postulated that genetic divergence between populations could result in genomic incompatibilities that would cause an increase in transposition in their hybrids, producing secondary effects such as sterility and therefore starting a speciation process. it has been demonstrated that transposition largely depends on intraspecific hybridization for p, hobo, and i elements in drosophila melanogaster and for several elements, including long terminal repeat (ltr) and non-ltr retrot ... | 1999 | 10406110 |
| effects of inbreeding in three life stages of drosophila buzzatii after embryos were exposed to a high temperature stress. | the interaction between inbreeding and high-temperature stress was examined in the cactophilic fruit fly, drosophila buzzatii. embryos of four inbreeding levels (f = 0, f = 0.25, f = 0.375, f = 0.5) were either maintained at 25 degrees c throughout egg-to-adult development or were exposed to 41.5 degrees c for 110 min at an age of 20 h. hatching, larva-to-pupa survival, pupa-to-adult survival, and egg-to-adult survival were estimated. heat shock reduced hatching rates, but survival to adulthood ... | 1997 | 9134706 |
| heat-shock tolerance and inbreeding in drosophila buzzatii. | the effect of inbreeding on survival after a short-term heat shock was tested for two age groups of the cactophilic fruit fly, drosophila buzzatii, reared under nonstress conditions. four inbreeding levels (f = 0, f = 0.25, f = 0.375, f = 0.5) were generated by outcrossing or full-sib mating. all flies were conditioned at 36.5 degrees c for 75 min prior to exposure to stress, to activate the synthesis of heat-shock proteins. these proteins are known to protect cells against stress damage. the yo ... | 1995 | 7706108 |
| drosophila adult and larval pheromones modulate larval food choice. | insects use chemosensory cues to feed and mate. in drosophila, the effect of pheromones has been extensively investigated in adults, but rarely in larvae. the colonization of natural food sources by drosophila buzzatii and drosophila simulans species may depend on species-specific chemical cues left in the food by larvae and adults. we identified such chemicals in both species and measured their influence on larval food preference and puparation behaviour. we also tested compounds that varied be ... | 2014 | 24741012 |
| chemical cues influence pupation behavior of drosophila simulans and drosophila buzzatii in nature and in the laboratory. | in the wild, larvae of several species of drosophila develop in heterogeneous and rapidly changing environments sharing resources as food and space. in this scenario, sensory systems contribute to detect, localize and recognize congeners and heterospecifics, and provide information about the availability of food and chemical features of environments where animals live. we investigated the behavior of d. simulans and d. buzzatii larvae to chemicals emitted by conspecific and heterospecific larvae ... | 2012 | 22737236 |
| sequence analysis, chromosomal distribution and long-range organization show that rapid turnover of new and old pbum satellite dna repeats leads to different patterns of variation in seven species of the drosophila buzzatii cluster. | we aimed to study patterns of variation and factors influencing the evolutionary dynamics of a satellite dna, pbum, in all seven drosophila species from the buzzatii cluster (repleta group). we analyzed 117 alpha pbum-1 (monomer length 190 bp) and 119 composite alpha/beta (370 bp) pbum-2 repeats and determined the chromosome location and long-range organization on dna fibers of major sequence variants. such combined methodologies in the study of satdnas have been used in very few organisms. in m ... | 2008 | 18266060 |
| low rates of homogenization of the dbc-150 satellite dna family restricted to a single pair of microchromosomes in species from the drosophila buzzatii cluster. | a satellite dna family, termed dbc-150, comprises slightly gc-rich repeat units of approximately 150 bp that were isolated (by dna digestions or pcr) from the genome of all seven drosophila species from the buzzatii cluster (repleta group). the presence of subrepeats suggests that part of the extant dbc-150 monomer originated by the duplication of small sequence motifs. the dbc-150 family is compared to the previously described pbum satdna family, an abundant component of the genome of five spec ... | 2007 | 17551842 |
| cactophilic drosophila in south america: a model for evolutionary studies. | the drosophila buzzatii cluster is composed of seven cactophilic species and their known geographical distribution encompasses the open vegetation diagonal, which includes the morphoclimatic domains of the caatinga, chaco and cerrado, which are situated between the amazon and the atlantic forests. besides these areas, these cactophilic species are also found in a narrow strip along the atlantic coast from northeastern brazil to the southern tip of the country. the hypothesis of vicariant events, ... | 2006 | 16502085 |
| evolutionary turnover of two pbum satellite dna subfamilies in the drosophila buzzatii species cluster (repleta group): from alpha to alpha/beta arrays. | the pbum satellite dna family was studied in seven drosophila species from the buzzatii cluster (within the large drosophila repleta group). the pbum repeats are slightly at-rich and show high levels of intraspecific sequence homogeneity. the pbum family can be divided into two subfamilies. the pbum-1 subfamily consists of tandemly arranged repetition units of approximately 190 bp, termed alpha. alpha repeats were found in a high copy number in the genome of d. buzzatii, d. serido and d. antonie ... | 2005 | 15777676 |
| evaluation of the courtship and of the hybrid male sterility among drosophila buzzatii cluster species (diptera, drosophilidae). | in the drosophila repleta group the establishment of subgroups and complexes made on the basis of morphological and cytological evidences is supported by tests of reproductive isolation. among species in the repleta group, the buzzatii cluster, due to its polymorphism and polytipism, is an excellent material for ecological and speciation studies. some interspecific crosses involving drosophila seriema, drosophila sp. b, d. koepferae and d. buzzatii strains were completely sterile while others in ... | 2002 | 12659009 |
| reproductive compatibility and chromosome pairing in the drosophila buzzatii complex. | fertility (percentage of fertile crosses) and the degree of synapsis in salivary gland chromosomes in isofemale lines of drosophila buzzatii, d. serido, d. koepferae and d. seriema were analysed. d. buzzatii was completely sterile in intercrosses with strains from the other species except for d. koepferae. the other species intercrossed to a greater or lesser degree, but also differed between crossing directions. homologous pairing in salivary gland preparations of strains and hybrids conformed ... | 1997 | 9297813 |
| the retrotransposon osvaldo from drosophila buzzatii displays all structural features of a functional retrovirus. | the osvaldo retrotransposon has shown a high transposition rate in some strains of drosophila buzzatii and in hybrids between d. buzzatii and its sibling d. koepferae. in order to understand the molecular basis of this phenomenon, we developed a procedure to clone a recently transposed copy with the aim of characterizing an active, full- length osvaldo element. the complete nucleotide sequence of osvaldo, obtained from a recent insertion site, was determined. osvaldo is 9,045 bp long and is comp ... | 1999 | 10406108 |
| a biophysical interpretation of temperature-dependent body size in drosophila aldrichi and d. buzzatii. | the temperature-size rule, the observation that most ectotherms grow faster but reach smaller size at higher temperatures, has defied a general explanation. here, the temperature-size rule in drosophila aldrichi and drosophila buzzatii is investigated, using data on development rate and adult dry weight at nine temperatures. in both species the linear regression of dry weight on temperature is negative. the data are used to infer the potential for a description of temperature dependent size by b ... | 2010 | 28799917 |
| transposable element misregulation is linked to the divergence between parental pirna pathways in drosophila hybrids. | interspecific hybridization is a genomic stress condition that leads to the activation of transposable elements (tes) in both animals and plants. in hybrids between drosophila buzzatii and drosophila koepferae, mobilization of at least 28 tes has been described. however, the molecular mechanisms underlying this te release remain poorly understood. to give insight on the causes of this te activation, we performed a te transcriptomic analysis in ovaries (notorious for playing a major role in te si ... | 2017 | 28854624 |
| pupal emergence pattern in cactophilic drosophila and the effect of host plants. | drosophila buzzatii and d. koepferae are sibling cactophilic species. the former breeds primarily on prickly pears (genus opuntia) whereas the latter breeds on columnar cacti of the genera cereus and trichocereus, although with certain degree of niche overlapping. we examined the interspecific differences in diurnal temporal patterns of adult emergence from puparia and evaluated whether this behavior is affected by rearing in the different cactus hosts available in nature. we detected important ... | 2017 | 28544122 |
| the role of courtship song in female mate choice in south american cactophilic drosophila. | courtship songs have undergone a spectacular diversification in the drosophila buzzatii cluster. accordingly, it has been suggested that sexual selection has played a significant role in promoting rapid diversification, reproductive isolation and speciation. however, there is no direct evidence (i.e., song playback experiments with wingless males) supporting this tenet. moreover, several studies have showed that the courtship song in the genus drosophila is not always used in female mate choice ... | 2017 | 28467464 |
| enhanced fertility and chill tolerance after cold-induced reproductive arrest in females of temperate species of the drosophila buzzatii complex. | long-term exposure to low temperatures during adult maturation might decrease fertility after cold recovery as a consequence of carry-over effects on reproductive tissues. this pattern should be more pronounced in tropical than in temperate species as protective mechanisms against chilling injuries are expected to be more effective in the latter. we initially determined the lower thermal thresholds to induce ovarian maturation in four closely related drosophila species, two inhabiting temperate ... | 2017 | 27956482 |
| transcriptome modulation during host shift is driven by secondary metabolites in desert drosophila. | high-throughput transcriptome studies are breaking new ground to investigate the responses that organisms deploy in alternative environments. nevertheless, much remains to be understood about the genetic basis of host plant adaptation. here, we investigate genome-wide expression in the fly drosophila buzzatii raised in different conditions. this species uses decaying tissues of cactus of the genus opuntia as primary rearing substrate and secondarily, the necrotic tissues of the columnar cactus t ... | 2016 | 27483442 |
| exploration of the drosophila buzzatii transposable element content suggests underestimation of repeats in drosophila genomes. | many new drosophila genomes have been sequenced in recent years using new-generation sequencing platforms and assembly methods. transposable elements (tes), being repetitive sequences, are often misassembled, especially in the genomes sequenced with short reads. consequently, the mobile fraction of many of the new genomes has not been analyzed in detail or compared with that of other genomes sequenced with different methods, which could shed light into the understanding of genome and te evolutio ... | 2016 | 27164953 |
| phenotypic plasticity in drosophila cactophilic species: the effect of competition, density, and breeding sites. | changes in the environmental conditions experienced by naturally occurring populations are frequently accompanied by changes in adaptive traits allowing the organism to cope with environmental unpredictability. phenotypic plasticity is a major aspect of adaptation and it has been involved in population dynamics of interacting species. in this study, phenotypic plasticity (i.e., environmental sensitivity) of morphological adaptive traits were analyzed in the cactophilic species drosophila buzzati ... | 2017 | 27061856 |
| drosophila females undergo genome expansion after interspecific hybridization. | genome size (or c-value) can present a wide range of values among eukaryotes. this variation has been attributed to differences in the amplification and deletion of different noncoding repetitive sequences, particularly transposable elements (tes). tes can be activated under different stress conditions such as interspecific hybridization events, as described for several species of animals and plants. these massive transposition episodes can lead to considerable genome expansions that could ultim ... | 2016 | 26872773 |
| changes of osvaldo expression patterns in germline of male hybrids between the species drosophila buzzatii and drosophila koepferae. | hybridization between different genomes is a source of genomic instability, sometimes associated with transposable element (te) mobilization. previous work showed that hybridization between the species drosophila buzzatii and drosophila koepferae induced mobilization of different (tes), the osvaldo retrotransposon being the most unstable. however, we ignore the mechanisms involved in this transposition release in interspecific hybrids. in order to disentangle the mechanisms involved in this proc ... | 2015 | 25711309 |
| genomics of ecological adaptation in cactophilic drosophila. | cactophilic drosophila species provide a valuable model to study gene-environment interactions and ecological adaptation. drosophila buzzatii and drosophila mojavensis are two cactophilic species that belong to the repleta group, but have very different geographical distributions and primary host plants. to investigate the genomic basis of ecological adaptation, we sequenced the genome and developmental transcriptome of d. buzzatii and compared its gene content with that of d. mojavensis and two ... | 2014 | 25552534 |
| host alkaloids differentially affect developmental stability and wing vein canalization in cactophilic drosophila buzzatii. | host shifts cause drastic consequences on fitness in cactophilic species of drosophila. it has been argued that changes in the nutritional values accompanying host shifts may elicit these fitness responses, but they may also reflect the presence of potentially toxic secondary compounds that affect resource quality. recent studies reported that alkaloids extracted from the columnar cactus trichocereus terscheckii are toxic for the developing larvae of drosophila buzzatii. in this study, we tested ... | 2014 | 25366093 |
| selection at the esterase-2 locus of drosophila buzzatii? perturbation-reperturbation experiments. | apparent selection affecting starch gel electrophoretic alleles at the esterase-2 locus of drosophila buzzatii has been detected in laboratory and natural populations. perturbation-reperturbation of allele frequencies in replicated laboratory populations attempts to test direct selective effects at the locus versus effects of linked loci. sequential gel electrophoresis has identified more alleles within starch classes, and three of these alleles (within the a, b and c starch alleles) were used i ... | 2014 | 25250904 |
| interspecific hybridization as a genomic stressor inducing mobilization of transposable elements in drosophila. | transposable elements (tes) are dna sequences able to be mobilized in host genomes. they are currently recognized as the major mutation inducers because of their insertion in the target, their effect on neighboring regions, or their ectopic recombination. a large number of factors including chemical and physical factors as well as intraspecific crosses have traditionally been identified as inducers of transposition. besides environmental factors, interspecific crosses have also been proposed as ... | 2014 | 25136509 |
| genome-wide dissection of hybrid sterility in drosophila confirms a polygenic threshold architecture. | to date, different studies about the genetic basis of hybrid male sterility (hms), a postzygotic reproductive barrier thoroughly investigated using drosophila species, have demonstrated that no single major gene can produce hybrid sterility without the cooperation of several genetic factors. early work using hybrids between drosophila koepferae (dk) and drosophila buzzatii (db) was consistent with the idea that hms requires the cooperation of several genetic factors, supporting a polygenic thres ... | 2014 | 24489077 |
| multiple paternity and sperm competition in the sibling species drosophila buzzatii and drosophila koepferae. | sperm competition (sc) is a major component of sexual selection that enhances intra- and intersexual conflicts and may trigger rapid adaptive evolution of sexual characters. the actual role of sc on rapid evolution, however, is poorly understood. besides, the relative contribution of distinctive features of the mating system to among species variation in the strength of sc remains unclear. here, we assessed the strength of sc and mating system factors that may account for it in the closely relat ... | 2013 | 23952173 |
| effects of dietary composition on life span of drosophila buzzatii and its short-lived sibling species d. koepferae. | two sibling drosophila species dramatically divergent in longevity, drosophila buzzatii and d. koepferae, were examined for possible effects of both developmental culture medium and dietary composition (dc) on longevity. longevity was greatly increased in the longer lived d. buzzatii when flies were reared and fed on a rich-in-nutrient and cactus-based culture (r-cbc) as compared to longevity in a poor nutrient culture (pnc). in d. buzzatii, life span was further increased by exposing flies to s ... | 2013 | 23835870 |
| inter and intraspecific variation in female remating propensity in the cactophilic sibling species drosophila buzzatii and d. koepferae. | post-mating sexual selection by means of sperm competition or cryptic female choice occurs in species in which females remate before exhausting sperm supplied by previous mates. thus, sperm competition is expected to be stronger when inseminated females remate more frequently or take longer to deplete sperm load. previous studies comparing oviposition behavior in the pair of closely related species drosophila buzzatii and drosophila koepferae suggest that inseminated females of the latter deplet ... | 2013 | 23542152 |
| striking structural dynamism and nucleotide sequence variation of the transposon galileo in the genome of drosophila mojavensis. | | 2013 | 23374229 |
| aedeagal divergence in sympatric populations of two sibling species of cactophilic drosophila (diptera: drosophilidae): evidence of character displacement? | aedeagal morphology of two sibling cactophilic species, drosophila buzzatii patterson & wheeler and drosophila koepferae fontdevila & wasserman, was analyzed in nine allopatric and three sympatric locations throughout south america. morphological differences were detected for both aedeagus size and shape between sympatric and allopatric populations of d. buzzatii, despite the significant variability within both groups. populations of d. buzzatii sympatric with d. koepferae displayed smaller aede ... | 2012 | 23950045 |
| segmental duplication, microinversion, and gene loss associated with a complex inversion breakpoint region in drosophila. | chromosomal inversions are usually portrayed as simple two-breakpoint rearrangements changing gene order but not gene number or structure. however, increasing evidence suggests that inversion breakpoints may often have a complex structure and entail gene duplications with potential functional consequences. here, we used a combination of different techniques to investigate the breakpoint structure and the functional consequences of a complex rearrangement fixed in drosophila buzzatii and comprisi ... | 2012 | 22328714 |
| effective population size of natural populations of drosophila buzzatii, with a comparative evaluation of nine methods of estimation. | allozyme and microsatellite data from numerous populations of drosophila buzzatii have been used (i) to determine to what degree n(e) varies among generations within populations, and among populations, and (ii) to evaluate the congruence of four temporal and five single-sample estimators of n(e) . effective size of different populations varied over two orders of magnitude, most populations are not temporally stable in genetic composition, and n(e) showed large variation over generations in some ... | 2011 | 21951766 |
| variations on a theme: diversification of cuticular hydrocarbons in a clade of cactophilic drosophila. | we characterized variation and chemical composition of epicuticular hydrocarbons (chcs) in the seven species of the drosophila buzzatii cluster with gas chromatography/mass spectrometry. despite the critical role of chcs in providing resistance to desiccation and involvement in communication, such as courtship behavior, mating, and aggregation, few studies have investigated how chc profiles evolve within and between species in a phylogenetic context. we analyzed quantitative differences in chc p ... | 2011 | 21699713 |
| adaptation of the aflp technique as a new tool to detect genetic instability and transposition in interspecific hybrids. | an adapted amplified fragment length polymorphism (aflp) protocol is presented for detection of hybrid instability in the genome of interspecific hybrids between drosophila buzzatii and d. koepferae species. analyses of 15 aflp instability markers (new bands detected in hybrids) show that up to 81% are the result of transposable element (te) activity. twenty tes associated with aflp instability markers have been detected by this method in backcross hybrids and segmental hybrids, demonstrating it ... | 2011 | 21548908 |
| osvaldo and isis retrotransposons as markers of the drosophila buzzatii colonisation in australia. | transposable elements (tes) constitute an important source of genetic variability owing to their jumping and regulatory properties, and are considered to drive species evolution. several factors that are able to induce te transposition in genomes have been documented (for example environmental stress and inter- and intra-specific crosses) but in many instances the reasons for te mobilisation have yet to be elucidated. colonising populations constitute an ideal model for studying te behaviour and ... | 2011 | 21513573 |
| testing chromosomal phylogenies and inversion breakpoint reuse in drosophila. the martensis cluster revisited. | the chromosomal relationships of the four martensis cluster species are among the most complex and intricate within the entire drosophila repleta group, due to the so-called sharing of inversions. here, we have revised these relationships using comparative mapping of bacterial artificial chromosome (bac) clones on the salivary gland chromosomes. a physical map of chromosome 2 of drosophila uniseta (one of the cluster members) was generated by in situ hybridization of 82 bac clones from the physi ... | 2011 | 21394512 |
| altitudinal and seasonal variation in microsatellite allele frequencies of drosophila buzzatii. | variation in climate, particularly temperature, is known to affect the genetic composition of populations. although there have been many studies of latitudinal variation, comparisons of populations across altitudes or seasons, particularly for animal species, are less common. here, we study genetic variation (microsatellite markers) in populations of drosophila buzzatii collected along altitudinal gradients and in different seasons. we found no differences in genetic variation between 2 years or ... | 2011 | 21091575 |
| geographic patterns of inversion polymorphism in the second chromosome of the cactophilic drosophila buzzatii from northeastern argentina. | the inversion polymorphisms of the cactophilic drosophila buzzatti patterson and wheeler (diptera: drosophilidae) were studied in new areas of its distribution in argentina. a total of thirty-eight natural populations, including 29 from previous studies, were analyzed using multiple regression analyses. the results showed that about 23% of total variation was accounted for by a multiple regression model in which only altitude contributed significantly to population variation, despite the fact th ... | 2010 | 21062144 |
| characterization and genomic organization of peri, a repetitive dna in the drosophila buzzatii cluster related to dine-1 transposable elements and highly abundant in the sex chromosomes. | transposable elements (tes) are dynamic components of eukaryotic genomes. we aimed to characterize tes to help elucidate their impact on the genomic architecture, diversity and evolution of chromosomes in the d. buzzatii cluster of species (repleta group). | 2011 | 20938165 |
| ontogenetic stage-dependent effect of temperature on developmental and metabolic rates in a holometabolous insect. | different hypotheses attempt to explain how different stages of organisms with complex life cycles respond to environmental changes. most studies have focused at the among-species level showing similar responses to temperature throughout ontogeny. however, there is no agreement about the pattern expected at the intraspecific scale where a strong selective effect is expected. in this paper, we studied the effects of thermal treatments on a life history trait (developmental rate) and a physiologic ... | 2010 | 20615413 |
| evolutionary dynamics and sites of illegitimate recombination revealed in the interspersion and sequence junctions of two nonhomologous satellite dnas in cactophilic drosophila species. | satellite dna (satdna) is a major component of genomes but relatively little is known about the fine-scale organization of unrelated satdnas residing at the same chromosome location, and the sequence structure and dynamics of satdna junctions. we studied the organization and sequence junctions of two nonhomologous satdnas, pbum and dbc-150, in three species from the neotropical drosophila buzzatii cluster (repleta group). in situ hybridization to microchromosomes, interphase nuclei and extended ... | 2009 | 19259119 |
| cloning and sequencing of the breakpoint regions of inversion 5g fixed in drosophila buzzatii. | chromosomal inversions are ubiquitous in drosophila both as intraspecific polymorphisms and interspecific differences. many gaps still remain in our understanding of the mechanisms that generate them. previous work has shown that in drosophila buzzatii, three polymorphic inversions were generated by ectopic recombination between copies of the transposon galileo. in this study, we have characterized the breakpoint regions of inversion 5g, fixed in d. buzzatii and absent in drosophila koepferae an ... | 2009 | 19198866 |
| the impact of genetic parental distance on developmental stability and fitness in drosophila buzzatii. | measures of genetic parental distances (gpd) based on microsatellite loci (d(2) and ir), have been suggested to be better correlated with fitness than individual heterozygosity (h), as they contain information about past events of inbreeding or admixture. we investigated if gpd increased with increasing genetic divergence between parental populations in drosophila buzzatii and if the measures indicate past events of admixture. further we evaluated the relationship between gpd, fitness and fluctu ... | 2008 | 18060507 |
| a study of wing morphology and fluctuating asymmetry in interspecific hybrids between drosophila buzzatii and d. koepferae. | in this work we investigate the effect of interspecific hybridization on wing morphology using geometric morphometrics in the cactophilic sibling species d. buzzatii and d. koepferae. wing morphology in f1 hybrids exhibited an important degree of phenotypic plasticity and differs significantly from both parental species. however, the pattern of morphological variation between hybrids and the parental strains varied between wing size and wing shape, across rearing media, sexes, and crosses, sugge ... | 2008 | 17647081 |
| evolution of male genitalia: environmental and genetic factors affect genital morphology in two drosophila sibling species and their hybrids. | the rapid evolution of genital morphology is a fascinating feature that accompanies many speciation events. however, the underlying patterns and explanatory processes remain to be settled. in this work we investigate the patterns of intraspecific variation and interspecific divergence in male genitalic morphology (size and shape) in the cactophilic sibling species drosophila buzzatii and d. koepferae. genital morphology in interspecific hybrids was examined and compared to the corresponding pare ... | 2007 | 17504529 |
| molecular population genetics of the alpha-esterase5 gene locus in original and colonized populations of drosophila buzzatii and its sibling drosophila koepferae. | several studies have suggested that esterase-2 (est-2) may be the target of natural selection in the cactophilic fly drosophila buzzatii. in this work, we analyzed nucleotide variation in a fragment of alpha-esterase5 (alphae5), the gene encoding est-2, in original (argentinian) and colonized (australian) populations of d. buzzatii and in its sibling d. koepferae. estimates of nucleotide heterozygosity in d. buzzatii were similar in australia and argentina, although we detected a loss of singlet ... | 2007 | 17160646 |
| the evolutionary history of drosophila buzzatii. xxxvi. molecular structural analysis of osvaldo retrotransposon insertions in colonizing populations unveils drift effects in founder events. | previous work on transposable element distribution in colonizing populations of drosophila buzzatii revealed a high frequency of occupancy in several chromosomal sites. two explanatory hypotheses were advanced: the founder hypothesis, by which founder genetic drift was responsible, and the unstable hypothesis that assigns this unusual distribution to bursts of transposition toward some chromosomal sites. here, we study the molecular structure of three euchromatic osvaldo clones isolated from sit ... | 2007 | 17151248 |
| molecular characterization and genomic distribution of isis: a new retrotransposon of drosophila buzzatii. | a new transposable element, isis, is identified as a ltr retrotransposon in drosophila buzzatii. dna sequence analysis shows that isis contains three long orfs similar to gag, pol and env genes of retroviruses. the orf1 exhibits sequence homology to matrix, capsid and nucleocapsid gag proteins and orf2 encodes a putative protease (pr), a reverse transcriptase (rt), an rnase h (rh) and an integrase (in) region. the analysis of a putative env product, encoded by the env orf3, shows a degenerated p ... | 2007 | 17039376 |
| testing chromosomal phylogenies and inversion breakpoint reuse in drosophila. | a combination of cytogenetic and bioinformatic procedures was used to test the chromosomal phylogeny relating drosophila buzzatii with d. repleta. chromosomes x and 2, harboring most of the inversions fixed between these two species, were analyzed. first, chromosomal segments conserved during the divergence of the two species were identified by comparative in situ hybridization to the d. repleta chromosomes of 180 bac clones from a bac-based physical map of the d. buzzatii genome. these conserve ... | 2007 | 17028333 |
| longevity differences among lines artificially selected for developmental time and wing length in drosophila buzzatii. | we assessed the indirect response of longevity in lines selected for wing length (wl) and developmental time (dt). longevity in selection lines was compared to laboratory control lines and the offspring of recently collected females. wild flies (w lines), flies from lines selected for fast development (f lines), and for fast development and large wing length (l lines) outlived control laboratory lines (c lines) and lines selected for fast development and short wing (s lines). the decline in long ... | 2006 | 16850224 |
| patterns of variation in wing morphology in the cactophilic drosophila buzzatii and its sibling d. koepferae. | drosophila buzzatii and d. koepferae are two sibling species that breed on the necrotic tissues of several cactus species and show a certain degree of niche overlap. also, they show differences in several life history traits, such as body size and developmental time, which probably evolved as a consequence of adaptation to different host plants. in this work we investigate the ecological and genetic factors affecting wing morphology variation both within and between species. three wing traits we ... | 2006 | 16780528 |