| independent replication of the ribosomal rna genes in the polytrophic-meroistic ovaries of calliphora erythrocephala, drosophila hydei, and sarcophaga barbata. | by filter saturation hybridizations the ribosomal (r)dna contents of the ovaries calliphora erythrocephala, drosophila hydei, and sarcophaga barbata have been measured in comparison to the rdna percentages of their diploid brains. the measurements of the ovarian rdna have been carried out on ovaries where the nurse cells in the distal egg chamber of the ovarioles had reached their highest ploidy level. the diploid rdna content of each of the respective species was chosen as a 100% standard and t ... | 1975 | 1201686 |
| characterization of the reverse transcriptase of 1731, a drosophila melanogaster retrotransposon. | the nucleotide sequence of 1731, a retrotransposon cloned from the genome of drosophila melanogaster, reveals a structural similarity with the proviral form of the retroviruses including a pol-like gene containing a putative reverse-transcriptase(rt)-coding sequence. diverse parts of that sequence were subcloned and expressed in escherichia coli. it has been demonstrated that the expression of the rt-like sequence, when translated, gives rise to peptides displaying enzyme activity characteristic ... | 1992 | 1385119 |
| laminin in the male germ cells of drosophila. | to study genes that may be crucial for the male germ cell development of drosophila we screened a cdna expression library with a polyclonal antiserum against testis proteins of drosophila hydei. we identified a cdna fragment that exhibited a complete sequence similarity with the cdna of the laminin b2 chain, an important component of the extracellular matrix. transcripts of laminin b2 were detected in the rna of male germ cells with the polymerase chain reaction and by in situ hybridization. we ... | 1992 | 1429843 |
| microdeletions in interval 6 of the y chromosome of males with idiopathic sterility point to disruption of azf, a human spermatogenesis gene. | for males with idiopathic sterility, a molecular screen specific for small lesions (microdeletions) in interval 6 of the y chromosome was set up using 29 y-dna probes. a "de novo" microdeletion in y interval 6 was detected in 2 out of 19 "chromosomally normal" sterile males. the first microdeletion includes the y-dna probes py6hp35 and 12f3; the second microdeletion includes the y-dna probes py6hp52, 49f, fr15-ii and the subinterval "c" of probe 50f2. a probe of the py6h sequence family is prese ... | 1992 | 1634226 |
| a potential z-dna-forming sequence is located between two transcription units alternatively expressed during development of drosophila hydei. | recent studies have demonstrated that z-dna exists in vivo in escherichia coli as well as in drosophila and mammalian cells. in the present paper, we show the existence in vivo of z-dna epitopes in the developmentally regulated subregion 4-75c of polytene chromosomes in drosophila hydei. the z-dna epitopes were detected in subdivision c2 only during late third instar when the transcriptional activity of the locus was high. accumulation of nonhistone chromosomal proteins in that locus was also de ... | 1991 | 1840694 |
| the alpha regulatory subunit of the mitochondrial f1-atpase complex is a heat-shock protein. identification of two highly conserved amino acid sequences among the alpha-subunits and molecular chaperones. | the recent identification of the alpha-subunit of mitochondrial f1-atpase complex in rat liver peroxisomes suggests another functional role for this protein in both organelles in addition to its involvement in mitochondrial oxidative phosphorylation. we report here that a very rapid response (15 min) in the induction of the alpha-regulatory subunit of the mitochondrial f1-atpase complex is observed in 37 degrees c heat-shocked larvae of drosophila hydei. under the same heat-shock treatment, a si ... | 1990 | 1970818 |
| selection of dna sequences from interval 6 of the human y chromosome with homology to a y chromosomal fertility gene sequence of drosophila hydei. | an experimental approach towards the molecular analysis of the male fertility function, located in interval 6 of the human y chromosome, is presented. this approach is not based on the knowledge of any gene product but on the assumption that the functional dna structure of male fertility genes, evolutionary conserved with their position on the y chromosome, may contain an evolutionary conserved frame structure or at least conserved sequence elements. we tested this hypothesis by using dhmif1, a ... | 1991 | 1999335 |
| oxidized p-phenylenediamine: observations on the staining reaction in epoxy embedded tissues. | p-phenylenediamine (ppd) is easily oxidized to brown compounds which stain acidic substrates. on account of the spontaneous oxidation process, the colour of ppd increases and becomes ochre-brown in a few days, showing an absorption peak at lambda = 510 nm with shoulder at about 440 to 460 nm. studies on the application of oxidized ppd as a stain for semi-thin sections revealed that some tissue components could be clearly visualized. after glutaraldehyde fixation, semi-thin and thin sections of a ... | 1986 | 2422692 |
| a group of non-y encoded drosophila hydei primary spermatocyte nuclear glycoproteins exhibits epitopes depending on formation of y chromosomal giant lampbrush loops. | in wild-type drosophila hydei (genotype x/y) four different primary spermatocyte nuclear glycoproteins, classified as non-y encoded because of their occurrence in x/o genotypes, were demonstrated to possess a few epitopes that depended on formation of the y chromosomal giant lampbrush loops threads (th; mr 55,000 proteins) or pseudonucleolus (ps; mr 38,000, 58,000 and 98,000 proteins). the epitopes reacted with lectins and/or antibodies in vitro lectin-/immunoreplica of primary spermatocyte tota ... | 1989 | 2476280 |
| differential riboflavin deposition in white and variegated white mutants of drosophila hydei. | riboflavin deposition in organs of drosophila hydei was studied by means of a growth test using a riboflavin-deficient strain of the fungus aspergillus nidulans. in wild-type animals, riboflavin is deposited in malpighian tubules (mt) and testes but not in adult eyes. certain white (w) mutants do not contain riboflavin, whereas intermediately colored w mutants contain minor amounts of the substance. riboflavin-containing mt cells contain special globules that can be fixed and stained with the re ... | 1987 | 3502968 |
| differential chromosomal distribution of ribonucleoprotein antigens in nuclei of drosophila spermatocytes. | the ribonucleoprotein (rnp) composition of the active y chromosomal structures in spermatocyte nuclei of drosophila hydei has been investigated using the anti-rnp antibodies dm 28k2 and pp60 as a probe. antibody dm 28k2 was raised against an rnp protein of cytoplasmic rnp particles in d. melanogaster cells, while antibody pp60 was raised against a pre-messenger rnp fraction from oocytes of xenopus laevis. both antibodies detect nuclear rnp (nrnp) antigens of d. hydei. this is shown by cscl densi ... | 1986 | 3536959 |
| purification and molecular characterization of alcohol dehydrogenase from drosophila hydei: conservation in the biochemical features of the enzyme in several species of drosophila. | alcohol dehydrogenase (adh) has been purified from drosophila hydei. biochemical investigations show that the native enzyme is a dimer consisting of two identical subunits with molecular weight 27,000. the ph optimum values of pure enzyme preparations are 7.9 and 9.4. the pi values are 8.83 and 8.41. substrate specificities have been characterized. km(app) values are lowest for propan-2-ol and butan-2-ol and vmax(app) values are highest for these two substrates. the amino acid composition has be ... | 1985 | 3910022 |
| regulated expression of a drosophila melanogaster heat shock locus after stable integration in a drosophila hydei cell line. | dna-mediated cotransformation has been used to transfer a drosophila melanogaster heat shock locus into cultured drosophila hydei cells by use of the copia-based selectable vector pcv2gpt and of pmh10a, a cloned 87a7 heat shock locus encoding a mutant heat shock protein (hsp). transformed lines contain between 50 and 200 copies of both plasmids, each separately organized as a head-to-tail concatemer which is stably maintained in the transformed lines. exposure of the cotransformants to heat shoc ... | 1985 | 3939313 |
| an assay for transient gene expression in transfected drosophila cells, using [3h]guanine incorporation. | we have developed an assay for transient gene expression using a dominant-selectable marker previously employed to transform drosophila cultured cells. drosophila hydei cells transfected with a functional escherichia coli xanthine guanine phosphoribosyl transferase gene (gpt), under the control of the long terminal repeats (ltrs) of the copia transposable element, rapidly incorporate guanine into acid-precipitable counts. autoradiographic analysis in situ shows that approximately 20% of cells ta ... | 1984 | 6096129 |
| the unusual structure of heat shock locus 2-48b in drosophila hydei. | we have previously isolated a 500 bp-long cdna clone, no9-15, which is derived from a nuclear transcript originating from the heat shock locus 2-48b of drosophila hydei (peters et al. 1982). sequence analysis shows that this clone carries 4 complete copies and 1 partial copy of a 115 bp repeat unit. the repeats are closely homologous with a maximal sequence divergence of about 10%. the sequence does not contain an open reading frame. the genomic organization of heat shock locus 2-48b, as probed ... | 1984 | 6597335 |
| in situ transcription analysis of chromatin template activity of the s-chromosome of drosophila following high molar nacl treatment. | the chromatin template activity of the polytene chromosomes in larval salivary glands of drosophila hydei has been assayed by in situ transcription, on the fixed chromatin using e. coli rna polymerase holoenzyme and 3h-utp as the monitoring substrate, both with optimal salt and high salt concentration (1 m nacl). results reveal an increase in the net transcription of all chromosomes in the high salt treatment series in comparison with the control. the x-chromosomes of the male larval gland also ... | 1981 | 7021083 |
| chromosomal basis of dosage compensation in drosophila. x. assessment of hyperactivity of the male x in situ. | the results of examination of the template activity of the fixed polytene chromosomes of drosophila hydei, monitored by 3h-utp, under in situ assay conditions, upon the use of endogenous drosophila polymerase, exogenous escherichia coli rna polymerase (holoenzyme) and exogenous drosophila rna polymerase ii (or b) have been presented. analysis of the data reveals that the transcription patterns with the 3 enzymes are not strictly comparable with the pattern obtained under in vivo conditions. yet, ... | 1981 | 7263783 |
| the drosophila micropia retrotransposon encodes a testis-specific antisense rna complementary to reverse transcriptase. | the micropia transposable element of drosophila hydei is a long terminal repeat-containing retrotransposon present in both the autosomes and the y chromosome. micropia expression gives rise to a complex set of sense and antisense rnas transcribed primarily during spermatogenesis. the most abundant sense rnas constitute an assortment of heterogeneous high-molecular-weight transcripts expressed as constituents of the y-chromosomal lampbrush loops of primary spermatocytes. in addition, micropia enc ... | 1994 | 7509447 |
| structure and expression of histone h3.3 genes in drosophila melanogaster and drosophila hydei. | we demonstrate that in drosophila melanogaster the histone h3.3 replacement variant is encoded by two genes, h3.3a and h3.3b. we have isolated cdna clones for h3.3a and cdna and genomic clones for h3.3b. the genes encode exactly the same protein but are widely divergent in their untranslated regions (utr). both genes are expressed in embryos and adults; they are expressed in the gonads as well as in somatic tissues of the flies. however, only one of them, h3.3a, shows strong testes expression. t ... | 1995 | 7557364 |
| interspecific sequence comparison of the muscle-myosin heavy-chain genes from drosophila hydei and drosophila melanogaster. | the muscle-myosin heavy-chain (mmhc) gene of drosophila hydei has been sequenced completely (size 23.3 kb). the sequence comparison with the d. melanogaster mmhc gene revealed that the exon-intron pattern is identical. the protein coding regions show a high degree of conservation (97%). the alternatively spliced exons (3a-b, 7a-d, 9a-c, 11a-e, and 15a-b) display more variations in the number of nonsynonymous and synonymous substitutions than the common exons (2, 4, 5, 6, 8, 10, 12, 13, 14, 16, 1 ... | 1994 | 7966366 |
| the anti z-dna reactivity of z-dna forming sequences is affected by platinum antitumor drugs. | the effect of binding of platinum antitumor drugs, cis-diamminedichloroplatinum (ii) (cis-ddp) and pt-pentamidine, on the z-dna reactivity of potential z-dna forming sequences has been studied by enzyme-linked immunosorbent assay. the results indicate that cis-ddp and pt-pentamidine increase the z-dna reactivity of plasmids containing a (dg-dc)16 insert (pucz8) and a native z-dna forming sequence of drosophila hydei (pf18). the molar ratio of platinum bound to nucleotides (rb) to produce 50% of ... | 1993 | 8227037 |
| a regulatory element of the empty spiracles homeobox gene is composed of three distinct conserved regions that bind regulatory proteins. | homeobox genes encode a class of highly evolutionarily conserved transcription factors that control embryonic development. the drosophila melanogaster empty spiracles gene is the homolog of the two human homeobox genes emx1 and emx2. these genes are necessary for central nervous system development. we used a regulatory element of the empty spiracles gene to study the control of homeobox gene expression. the 1.2-kilobase (kb) cis-regulatory element located 3 kb 5' of the transcription start site ... | 1998 | 9491376 |
| genome-wide insertional mutagenesis in human cells by the drosophila mobile element minos. | the development of efficient non-viral methodologies for genome-wide insertional mutagenesis and gene tagging in mammalian cells is highly desirable for functional genomic analysis. here we describe transposon mediated mutagenesis (tramm), using naked dna vectors based on the drosophila hydei transposable element minos. by simple transfections of plasmid minos vectors in hela cells, we have achieved high frequency generation of cell lines, each containing one or more stable chromosomal integrati ... | 2000 | 11258481 |
| in vivo transposition of minos, a drosophila mobile element, in mammalian tissues. | transposable elements have been used widely in the past 20 years for gene transfer and insertional mutagenesis in drosophila. transposon-based technology for gene manipulation and genomic analysis currently is being adopted for vertebrates. we tested the ability of minos, a dna transposon from drosophila hydei, to transpose in mouse tissues. two transgenic mouse lines were crossed, one expressing minos transposase in lymphocytes under the control of the cd2 promoter/locus control region and anot ... | 2001 | 11562481 |
| identification of some heat-induced genes of trichinella spiralis. | three heat-induced genes of the infective-stage larvae of trichinella spiralis were successfully identified by the suppression subtractive hybridization (ssh) technique. as indicated by reverse northern blotting, 19 of 25 clones were scored as differentially transcribed in the heat-shocked infective-stage larvae. the sequencing data showed the presence of 12 different genes. three were homologous to histone h3, histone h2b and translationally controlled tumour protein (tctp). a 0.6 kb cdna of hi ... | 2001 | 11578093 |
| transposition of the drosophila hydei minos transposon in the mouse germ line. | we tested the suitability of the fly transposon minos, a member of the tc1/mariner superfamily, for insertional mutagenesis in the mouse germ line. we generated a transgenic mouse line expressing minos transposase in growing oocytes and another carrying a tandem array of nonautonomous transposons. the frequency of transposition in the progeny derived from oocytes carrying both transgenes is 8.2%. analysis of the new integration sites shows a high frequency of transpositions to a different chromo ... | 2003 | 12620388 |
| transposable elements as tools for genomics and genetics in drosophila. | the p-element has been the workhorse of drosophila genetics since it was developed as a tool for transgenesis in 1982; the subsequent development of a variety of systems based on the transposon have provided a range of powerful and flexible tools for genetics and genomics applications. p-element insertions are frequently used as starting-points for generating chromosomal deletions to remove flanking genes, either by screening for imprecise excision events or by selecting for male recombination e ... | 2003 | 15239944 |
| minos as a genetic and genomic tool in drosophila melanogaster. | much of the information about the function of d. melanogaster genes has come from p-element mutagenesis. the major drawback of the p element, however, is its strong bias for insertion into some genes (hotspots) and against insertion into others (coldspots). within genes, 5'-utrs are preferential targets. for the successful completion of the drosophila genome disruption project, the use of transposon vectors other than p will be necessary. we examined here the suitability of the minos element fro ... | 2005 | 15972463 |
| high positive predictive value of specific antibodies cross-reacting with a 28-kda drosophila antigen for diagnosis of ankylosing spondylitis. | diagnosis of ankylosing spondylitis (as) can be difficult, and a specific laboratory test has not yet been introduced as a routine diagnostic tool. our aim was to evaluate the diagnostic value of antibodies specifically binding to a recombinant 28-kda antigen for the diagnosis of as. | 2006 | 16159948 |
| prevalence of a non-male-killing spiroplasma in natural populations of drosophila hydei. | male-killing phenotypes are found in a variety of insects and are often associated with maternally inherited endosymbiotic bacteria. in several species of drosophila, male-killing endosymbionts of the genus spiroplasma have been found at low frequencies (0.1 to 3%). in this study, spiroplasma infection without causing male-killing was shown to be prevalent (23 to 66%) in japanese populations of drosophila hydei. molecular phylogenetic analyses showed that d. hydei was infected with a single stra ... | 2006 | 17021218 |
| negative effects of low temperatures on the vertical transmission and infection density of a spiroplasma endosymbiont in drosophila hydei. | maternally transmitted endosymbionts of the genus spiroplasma infecting several species of drosophila are known to cause selective death of male offspring (male killing). the male-killing trait is considered to be advantageous for maternally transmitted endosymbionts. however, a non-male-killing spiroplasma is present in japanese populations of drosophila hydei at high frequencies (23-66%). this spiroplasma is phylogenetically closely related to the male-killing spiroplasma infecting other droso ... | 2008 | 18618173 |
| spiroplasma bacteria enhance survival of drosophila hydei attacked by the parasitic wasp leptopilina heterotoma. | maternally-transmitted associations between endosymbiotic bacteria and insects are ubiquitous. while many of these associations are obligate and mutually beneficial, many are facultative, and the mechanism(s) by which these microbes persist in their host lineages remain elusive. inherited microbes with imperfect transmission are expected to be lost from their host lineages if no other mechanisms increase their persistence (i.e., host reproductive manipulation and/or fitness benefits to host). in ... | 2010 | 20730104 |
| the effect of changes in the respiratory metabolism upon genome activity. a correlation between induced gene activity and an increase in activity of a respiratory enzyme. | the in vitro regression of experimentally induced chromosome puffs was investigated in explanted salivary gland chromosomes of drosophila hydei. it was observed that the regression of the puffs 2-32a, 2-36a, 2-48c, and 4-81b is accelerated if substrates for the respiratory metabolism are supplied to the cells. a similar effect can be produced by addition of kcn or oligomycin to medium in which intact salivary glands are incubated. the acceleration of puff regression by these substances occurs no ... | 2016 | 5076777 |
| y chromosome function and spermatogenesis in drosophila hydei. | | 2013 | 3887859 |
| local protein accumulation during gene activation. ii. interferometric measurements of the amount of solid material in temperature induced puffs of drosophila hydei. | | 2014 | 4929853 |
| purification of mitochondrial nadh dehydrogenase from drosophila hydei and comparison with the 'heat-shock' polypeptides. | mitochondrial nadh dehydrogenase (nadh:(acceptor) oxidoreductase, ec .6.99.3) from either drosophila hydei larvae or embryos has been purified 150- and 120-fold, respectively. the purified enzyme appeared homogeneous and showed a molecular weight of 57 000. the molecular weight of the nondenatured enzyme was 79 000. on isoelectro-focussing of the preparation, two fractions were observed, a major one with an isoelectric point of 6.2 and a minor fraction with an isoelectric point of 4.9. straight- ... | 1978 | 222325 |
| spermatogenesis of drosophila hydei. | | 2008 | 2289847 |
| evolution of y chromosomal lampbrush loop dna sequences of drosophila. | the evolutionary conservation of y chromosomal dna sequences of drosophila hydei in different species of the genus drosophila was studied by in situ hybridization and on genomic dna blots of restriction enzyme digested dna. we demonstrated that y specific dna sequences, which form major parts of lampbrush loops related to the male fertility genes, are only retained in a few closely related species during evolution. other y chromosomal dna sequences, also present in lampbrush loops but with homol ... | 2010 | 2881740 |
| differentiation of transplanted larval salivary glands of drosophila hydei in adults of the same species. | | 2014 | 4222967 |
| [differential radiosensitivity of the germ cells in drosophila hydei males, lethal rates and the relevance of the species-specific genetic organization (author's transl)]. | | 2015 | 4469156 |
| [regulation of ion concentration by drosophila hydei na+, k+, ca2+, and cl-]. | | 2015 | 4425097 |
| minos as a novel tc1/mariner-type transposable element for functional genomic analysis in aspergillus nidulans. | transposons constitute powerful genetic tools for gene inactivation, exon or promoter trapping and genome analyses. the minos element from drosophila hydei, a tc1/mariner-like transposon, has proved as a very efficient tool for heterologous transposition in several metazoa. in filamentous fungi, only a handful of fungal-specific transposable elements have been exploited as genetic tools, with the impala tc1/mariner element from fusarium oxysporum being the most successful. here, we developed a t ... | 2015 | 26021704 |
| structural and functional properties of polytene nuclei isolated from salivary glands of drosophila hydei. | salivary gland nuclei of drosophila hydei, isolated by a modification of the procedure described by boyd et al. (9), retain their normal morphology during the isolation and subsequent incubation procedure. rna synthesis was studied in isolated nuclei by biochemical and cytological techniques. in radioautographs 70% of the nuclei displayed a distribution of labeled rna over the nuclear constituents similar to the distribution obtained after in vivo incorporation of radioactive precursor. chromoso ... | 1969 | 5783875 |
| permanent genetic resources added to molecular ecology resources database 1 april 2013-31 may 2013. | this article documents the addition of 234 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: acipenser sinensis, aleochara bilineata, aleochara bipustulata, barbus meridionalis, colossoma macropomum, delia radicum, drosophila nigrosparsa, fontainea picrosperma, helianthemum cinereum, liomys pictus, megabalanus azoricus, pelteobagrus vachelli, pleuragramma antarcticum, podarcis hispanica type 1a, sardinella brasiliensis and scle ... | 2013 | 23937578 |
| mite choice generates sex- and size-biased infection in drosophila hydei. | heterogeneities in parasite infection among conspecific hosts often manifest as sex- or size-biased infections, which are typically attributed to differential host susceptibility and exposure. since parasite fitness is often tied to host quality, host preference by parasites is likely to be under strong selection. we test the hypothesis that host preference is sufficient to generate variability in infection rate among conspecifics. specifically, we ask whether the mite macrocheles muscaedomestic ... | 2016 | 26997035 |
| effect of tin and lead chlorotriphenyl analogues on fruit fly drosophila hydei and liposomes membrane. | this article presents the results of a study investigating the biological activity of triphenyltin chloride (tpht) and two metalloorganic compounds, triphenyllead chloride (tphl) and triphenylmethane chloride (tphc), in their interaction with model membranes and the living organisms of fruit flies drosophila hydei. the study of model membranes (sonicated liposomes) was conducted using the electron spin resonance (esr) spin probe technique, whereas the experiment on fruit flies involved investiga ... | 2013 | 22447721 |
| the embryonic and post-embryonic development in two drosophila species exposed to the static magnetic field of 60 mt. | in this study, a static magnetic field influence on development and viability in two different species, drosophila melanogaster and drosophila hydei, was investigated. both species completed development (egg-adult), in and out of the static magnetic field induced by double horseshoe magnet. treated vials with eggs were placed in the gap between magnetic poles (47 mm) and exposed to the average magnetic induction of 60 mt, while control ones were kept far enough from magnetic field source. we fou ... | 2011 | 21591895 |
| drosophila melanogaster kl-3 and kl-5 y-loops harbor triple-stranded nucleic acids. | primary spermatocyte nuclei of drosophila melanogaster contain three prominent lampbrush-like loops. the development of these structures has been associated with the transcription of three fertility factors located on the y chromosome, named kl-5, kl-3 and ks-1. these loci have huge physical dimensions and contain extremely long introns. in addition, kl-3 and kl-5 were shown to encode two putative dynein subunits required for the correct assembly of the sperm axoneme. here, we show that both the ... | 2008 | 18430782 |
| the endogenous drosophila melanogaster retrovirus gypsy can propagate in drosophila hydei cells. | the endogenous drosophila melanogaster retrovirus gypsy (mdg4) forms virus-like particles (vlps) which are found as extracellular particles in the medium used to culture d. melanogaster cells. the d. hydei somatic cell line dh14, which does not harbour gypsy sequences, was exposed to d. melanogaster vlps. subsequent pcr and southern analysis revealed that gypsy elements had penetrated into the d. hydei cells, suggesting interspecific transmission of the retrovirus. a d. hydei cell line containin ... | 2001 | 11212913 |
| evolution of gypsy endogenous retrovirus in the drosophila obscura species group. | the ty3/gypsy family of retroelements is closely related to retroviruses, and some of their members have an open reading frame resembling the retroviral gene env. sequences homologous to the gypsy element from drosophila melanogaster are widely distributed among drosophila species. in this work, we report a phylogenetic study based mainly on the analysis of the 5' region of the env gene from several species of the obscura group, and also from sequences already reported of d. melanogaster, drosop ... | 2000 | 10908638 |
| evolutionary change of codon usage for the histone gene family in drosophila melanogaster and drosophila hydei. | the nucleotide divergence in the protein-coding region for replication-dependent and replication-independent histone 3 and 4 genes of drosophila melanogaster and drosophila hydei occurred mostly at the synonymous site. therefore, the pattern of codon usage was analyzed in the two species, considering the genomic codon bias, which is proposed for estimating the genomic composition pressure in the protein-coding regions. the results indicated that the codon usage in the histone gene family could b ... | 2000 | 10837157 |
| proteins with tandemly arranged repeats of a highly charged 16-amino-acid motif encoded by the dhmst101 gene family are structural components of the outer sheath of the extremely elongated sperm tails of drosophila hydei. | fruit fly species of the genus drosophila show a remarkable variation in sperm length. some of them produce gigantic sperm several times the total male body length. sperm of drosophila hydei, for example, are more than 20 mm long. little is known about the advantage of such elongated sperm or about the proteins that stabilize their thin flagellar tails. recently, two members of a novel gene family dhmst101(1) and dhmst101(2), whose gene products are associated with the sperm tail, were isolated ... | 1999 | 10445506 |
| the y chromosomal fertility factor threads in drosophila hydei harbors a functional gene encoding an axonemal dynein beta heavy chain protein. | to understand the contradiction between megabase-sized lampbrush loops and putative protein encoding genes both associated with the loci of y chromosomal fertility genes of drosophila on the molecular level, we used pcr-mediated cloning to identify and isolate the cdna sequence of the y chromosomal drosophila hydei gene dhdhc7(y). alignment of the sequences of the putative protein dhdhc7(y) and the outer arm dynein beta heavy chain protein dyh2 of tripneustes gratilla shows homology over the ent ... | 1998 | 9649526 |
| molecular cloning and characterization of a transcription factor for the copia retrotransposon with homology to the btb-containing lola neurogenic factor. | by transfection experiments, we previously identified a 72-bp enhancer sequence within the drosophila copia retrotransposon which is involved in the control of the transcription level of this mobile element in cells in culture. gel shift assays with nuclear extracts from drosophila hydei-derived dh-33 cells further demonstrated specific interactions of at least two nuclear factors with this enhancer sequence. using this sequence as a probe for the screening of an expression cdna library that we ... | 1997 | 8972229 |
| molecular phylogeny and genome evolution in the drosophila virilis species group: duplications of the alcohol dehydrogenase gene. | drosophila virilis is a prominent reference species for comparison with drosophila melanogaster in regard to patterns and mechanisms of molecular and genomic evolution. sequences were determined for 11 adh genes from 8 species of the d. virilis species group, including species from both the virilis phylad and the montana subphylad. the genome of d. virilis contains a 6-kb duplication that includes the entire adh coding region. the pattern of sequence identity within the duplication strongly sugg ... | 1996 | 8583887 |
| gene transfer into the medfly, ceratitis capitata, with a drosophila hydei transposable element. | exogenous functional dna was introduced into the germline chromosomes of the mediterranean fruit fly (medfly) ceratitis capitata with a germline transformation system based on the transposable element minos from drosophila hydei. transformants were identified as phenotypic revertants of a white-eyed mutation carried by the recipient strain. clusters of transformants were detected among the progeny of 390 individuals screened for germline transformation. five independent and phenotypically active ... | 1995 | 8533094 |
| structure and regulation of the fushi tarazu gene from drosophila hydei. | the drosophila melanogaster segmentation gene fushi tarazu (ftz) encodes a homeodomain-type transcription factor involved in the control of larval pattern formation. loss of function mutations cause an embryonic lethal, pair-rule phenotype. the segmentation defects, but not the lethality, can be partially rescued by the ftz orthologue from drosophila hydei. in this work, the primary structure, expression and regulation of the d. hydei ftz gene was characterized. sequence comparisons classify ftz ... | 1995 | 28306077 |
| introduction of the transposable element minos into the germ line of drosophila melanogaster. | a transposon based on the transposable element minos from drosophila hydei was introduced into the genome of drosophila melanogaster using transformation mediated by the minos transposase. the transposon carries a wild-type version of the white gene (w) of drosophila inserted into the second exon of minos. transformation was obtained by injecting the transposon into preblastoderm embryos that were expressing transposase either from a hsp70-minos fusion inserted into the genome via p-element-medi ... | 1995 | 7568159 |
| large-male advantages associated with costs of sperm production in drosophila hydei, a species with giant sperm. | males of the fruit fly drosophila hydei were found to produce 23.47 +/- 0.46-mm-long spermatozoa, the longest ever described. no relationship was found between male body size and sperm length. we predicted that if these giant gametes are costly for males to produce, then correlations should exist between male body size, rates of sperm production, and fitness attributes associated with the production of sperm. smaller males were found to make a greater relative investment in testicular tissue gro ... | 1994 | 7937755 |
| identification of a strong transcriptional activator for the copia retrotransposon responsible for its differential expression in drosophila hydei and melanogaster cell lines. | we have characterized the regulatory properties of a 72bp sequence located in the 5' untranslated domain of the drosophila copia retrotransposon, 3' to the left ltr, by transient transfection assays with cell lines derived from either drosophila hydei (dh33 cells) or drosophila melanogaster (schneider ii and kc cells). reporter plasmids were constructed which contained the lacz gene under the control of either the entire copia ltr with 5' untranslated domain, or a minimal heterologous promoter f ... | 1994 | 8074683 |
| mobile minos elements from drosophila hydei encode a two-exon transposase with similarity to the paired dna-binding domain. | elements related to the tc1-like minos mobile element have been cloned from drosophila hydei and sequenced. southern blot and sequence analyses show that (i) the elements are actively transposing in the drosophila hydei germ line, (ii) they are characterized by a striking degree of sequence and size homogeneity, and (iii) like tc1, they insert at a ta dinucleotide that is probably duplicated during the process. the nucleotide sequences of two elements, minos-2 and minos-3, differ at only one pos ... | 1994 | 8197129 |
| evolutionary conservation of the structure and expression of alternatively spliced ultrabithorax isoforms from drosophila. | in drosophila melanogaster, alternatively spliced mrnas from the homeotic gene ultrabithorax (ubx) encode a family of structurally distinct homeoprotein isoforms. the developmentally regulated expression patterns of these isoforms suggest that they have specialized stage- and tissue-specific functions. to evaluate the functional importance of ubx isoform diversity and gain clues to the mechanism that regulates processing of ubx rnas, we have investigated whether the ubx rnas of other insects und ... | 1994 | 7911773 |
| hsp90 associates with specific heat shock puffs (hsr omega) in polytene chromosomes of drosophila and chironomus. | the heat shock protein hsp90, which is mainly cytoplasmic, has recently been reported to be present in the nucleus. we have found a specific chromosomal localization of hsp90 in different species of drosophila and chironomus using immunocytochemical techniques with different mono- and polyclonal antibodies for this hsp. hsp90 was found associated with heat shock-induced puffs at 93d and 48b in salivary gland chromosomes of drosophila melanogaster and drosophila hydei, respectively. the localizat ... | 1993 | 8306827 |
| an 18-bp element in the 5' untranslated region of the drosophila beta 2 tubulin mrna regulates the mrna level during postmeiotic stages of spermatogenesis. | during drosophila spermatogenesis transcriptional activity is mainly restricted to premeiotic stages. translation during sperm morphogenesis, however, proceeds for several days, requiring a high stability for mrnas translated postmeiotically. we studied expression of the drosophila beta 2 tubulin gene, which is expressed solely in the male germ line from the primary spermatocyte stage onwards. cis-acting elements involved in the regulation of mrna levels were investigated in transgenic fly strai ... | 1993 | 8269980 |
| the evolution of duplicate glyceraldehyde-3-phosphate dehydrogenase genes in drosophila. | in drosophila melanogaster there are two genes which encode the enzyme glyceraldehyde-3-phosphate dehydrogenase (gapdh), gapdh-43e and gapdh-13f. we have shown that gapdh-43e codes for the gapdh subunit with an apparently larger molecular weight while gapdh-13f encodes the gapdh subunit having an apparently smaller molecular weight. immunoblots of sodium dodecyl sulfate gels were used to survey species from throughout the genus and results indicated that two classes of gapdh subunits are present ... | 1992 | 1468631 |
| interspecific comparison of the transformer gene of drosophila reveals an unusually high degree of evolutionary divergence. | the transformer (tra) gene of drosophila melanogaster occupies an intermediate position in the regulatory pathway controlling all aspects of somatic sexual differentiation. the female-specific expression of this gene's function is regulated by the sex lethal (sxl) gene, through a mechanism involving sex-specific alternative splicing of tra pre-mrna. the tra gene encodes a protein that is thought to act in conjunction with the transformer-2 (tra-2) gene product to control the sex-specific process ... | 1992 | 1592233 |
| regulation of the segmentation gene fushi tarazu has been functionally conserved in drosophila. | an evolutionary approach was applied to identify elements involved in the regulation of the segmentation gene fushi tarazu (ftz) by comparing the drosophila melanogaster ftz gene with its drosophila hydei homologue. the overall organization of the ftz gene is very similar in both species. surprisingly, ftz proved to be inverted in the ant-c of d. hydei with respect to d. melanogaster. strong homologies extend over the entire 6 kb of the ftz upstream region with the best match in the 'upstream el ... | 1990 | 2174353 |
| dna sequence comparison of micropia transposable elements from drosophila hydei and drosophila melanogaster. | members of the retrotransposon family micropia were discovered as constituents of wild-type y chromosomal fertility genes from drosophila hydei. several members of the micropia family have subsequently been recovered from drosophila melanogaster and four micropia elements, micropia-dhmif2, -dhmif8, -dm11 and -dm2, two each from d. hydei and d. melanogaster, have been totally sequenced (17 kb of micropia sequences and 6.8 kb from insertions). comparative analysis of micropia sequences revealed a ... | 1990 | 2162752 |
| isolation and characterization of a drosophila hydei histone dna repeat unit. | histone genes in d. hydei are organized in tandemly repeated clusters., accomodating in total 120-140 repeat units. we cloned one of the repeat units and analysed the nucleotide sequence. the repeat unit has a size of 5.1 x 10(3) base-pairs and contains one copy of each of the genes coding for the core histones and one copy coding for the histone h1. in the promoter regions of the genes we identified the presumptive cap sites and tata boxes. two additional sequence elements are shared by all fiv ... | 1990 | 2109309 |
| conservatism of sites of trna loci among the linkage groups of several drosophila species. | the sites of seven trna genes (arg-2, lys-2, ser-2b, ser-7, thr-3, thr-4, val-3b) were studied by in situ hybridization. 125i-labeled trna probes from drosophila melanogaster were hybridized to spreads of polytene chromosomes prepared from four drosophila species representing different evolutionary lineages (d. melanogaster, drosophila hydei, drosophila pseudoobscura, and drosophila virilis). most trna loci occurred on homologous chromosomal elements of all four species. in some cases the number ... | 1990 | 2107332 |
| characterization of the long terminal repeats of micropia elements microdissected from the y-chromosomal lampbrush loops "threads" of drosophila hydei. | four micropia elements from drosophila melanogaster and d. hydei have been analysed by sequencing. two elements, from d. hydei, micropia-dhmif8 and -dhmif2, were recovered by cloning microdissected y-chromosomal lampbrush loops "threads". this method allows isolation of repetitive sequences from defined chromosomal positions, but recovery of large and overlapping inserts is difficult. in case of the y-chromosomal micropia elements it was not possible to define the endpoints of their long termina ... | 1989 | 2585498 |
| the transcriptional control regions of the copia retrotransposon. | we have analysed the sequence elements that control expression of the copia retrotransposon. expression of copia fusion constructs containing dna sequence deletions and rearrangements was assayed by transient expression analysis. progressive deletion and linker substitution identifies two regions on either side of the major transcriptional start sites in the copia long terminal repeat these regions are both required for high level expression in a cultured drosophila melanogaster cell line but on ... | 1989 | 2472602 |
| y chromosome loops in drosophila melanogaster. | primary spermatocyte nuclei of fixed testes of drosophila melanogaster exhibit three large clusters of thread-like structures, each consisting of two long, continuous, loop-shaped filaments. no comparable intranuclear structures are observed in spermatogonia, secondary spermatocytes or spermatids. the threads begin to form in young spermatocytes, grow throughout spermatocyte development, reach their maximum size in mature spermatocytes and disintegrate prior to meiotic metaphase i. the presence ... | 1988 | 2465201 |
| micropia: a retrotransposon of drosophila combining structural features of dna viruses, retroviruses and non-viral transposable elements. | the retrotransposon micropia was first described from y-chromosomal fertility genes of drosophila hydei. screening a drosophila melanogaster genomic library yielded several clones representing micropia elements in d. melanogaster. the dna sequences of two elements from d. hydei (micropia-dhmif2 and micropia-dhmif8) and two elements from d. melanogaster (micropia-dm2 and micropia-dm11) permitted a detailed analysis of the spatial organization of micropia constituents. micropia represents the typi ... | 1988 | 2464689 |
| heat shock loci 93d of drosophila melanogaster and 48b of drosophila hydei exhibit a common structural and transcriptional pattern. | a comparison of gene structure, sequence, and transcription pattern of heat shock loci 93d of drosophila melanogaster and 48b of drosophila hydei has been performed. both heat shock loci consist of an unique region that is flanked by an internally repetitive element. different members of these elements are highly conserved, repeat unit length, however, and primary sequence diverged totally. whereas the overall gene structure in both species is substantially related, sequence conservation is only ... | 1987 | 3106930 |
| a drosophila heat shock locus with a rapidly diverging sequence but a conserved structure. | cytological studies have shown that the heat shock loci 93d of drosophila melanogaster and 2-48b of drosophila hydei have several characteristics which suggest that they are homologous loci, yet sequence homology is barely detectable by cross-hybridization. using cloned dna sequences we have compared the two loci. both loci produce transcripts of similar size and number. we have characterized the three predominant transcripts. in each species all three transcripts start at or about the same plac ... | 1986 | 3097014 |
| heat shock locus 93d of drosophila melanogaster: a spliced rna most strongly conserved in the intron sequence. | the drosophila melanogaster heat shock locus at 93d encodes at least three overlapping transcripts, 10-12 kilobases (kb), 1.9 kb, and 1.2 kb. the abundance of the three transcripts is significantly increased during heat shock; however, all are also found in non-heat-shocked cells. the 1.2-kb transcript is found in the cytoplasm. sequence analysis of a 1.1-kb cdna clone representing sequences within the 1.2-kb transcript and comparison to genomic sequences indicate that it is spliced; 700 base pa ... | 1986 | 3081901 |
| the ribosomal dna of drosophila melanogaster is organized differently from that of drosophila hydei. | | 1983 | 6300412 |
| intervening sequences in ribosomal rna genes and bobbed phenotype in drosophila hydei. | the "bobbed' (bb) mutation in drosophila is represented phenotypically by shortened and abnormally thin scutellar bristles and by delayed development. there is a direct correlation between bristle size and ribosomal rna (rrna) synthesis, and the bb mutation was at first explained as a deficiency of rrna genes (rdna). however, the bb phenotype can occur in drosophila melanogaster and drosophila hydei with high rdna content, while phenotypically wild-type flies are known with few rrna genes, sugge ... | 1981 | 7254359 |
| isolation of a structural gene mapping to subregions 63f of drosophila melanogaster and 90b of d. hydei polytene chromosomes. | we have isolated by molecular cloning techniques a structural gene that maps to subregion 63f of drosophila melanogaster chromosome 3l. this locus being one of the early chromosomal targets for ecdysone stimulation, may be induced to puff by the hormone. the gene is on a plasmid vector and it has been designated as pdm 63f. this recombinant molecule also maps to the early ecdysone inducible subregion 90b of drosophila hydei chromosome 4. the cytological inspection of large number of chromosomal ... | 1981 | 6168445 |
| variations in the number of the y chromosomal rrna genes in drosophila hydei. | the number of rrna cistrons is measured by filter saturation hybridization in different stocks of d. hydei, where the wild-type x chromosome has one nucleolus organizer (no) and wild-type y has two separated no's. xx/y females having no x chromosomal no show an rdna content exceeding that of a y chromosome. an even greater increase in the rrna cistron number is measured in two translocation stocks where the xx is combined with one half of a y and, therefore, each stock contains only one of the t ... | 1976 | 814041 |
| pteridines and gene homologies in the eye color mutants of drosophila hydei and drosophila melanogaster. | | 1965 | 17248279 |
| arsenates: effect on fecundity in some diptera. | four species of adult diptera, rhagoletis pomonella (walsh), drosophila melanogaster mg., drosophila hydei sturt., and musca domestica l. showed varying degrees of reduced fecundity when sublethal doses of arsenic were included in their food. the possible influence of the frequent use of arsenicals on arthropod populations is discussed. | 1963 | 17829544 |
| [radiation sensitivity in early embryonal stages of drosophila hydei sturtevant in comparison to drosophila melanogaster meigen]. | | 1957 | 13519312 |
| indiscriminate females and choosy males: within- and between-species variation in drosophila. | the classic view of choosy, passive females and indiscriminate, competitive males gained theoretical foundations with parental investment theory. when females invest more in offspring than males, parental investment theory says that selection operates so that females discriminate among males for mates (i.e., females are choosy and passive) and males are indiscriminate (i.e., males are profligate and competitive). here we report tests of predictions using drosophila pseudoobscura and d. melanogas ... | 2003 | 14575325 |
| fluorescence in situ hybridization of ribosomal dna to mitotic chromosomes of tsetse flies (diptera: glossinidae: glossina). | ribosomal genes were mapped in glossina austeni, g. brevipalpis, g. f. fuscipes, g. m. submorsitans, g. p. palpalis, g. pallidipes and g. tachinoides on mitotic chromosomes by fluorescence in situ hybridization (fish) using drosophila hydei genomic clones that contain the 28s ribosomal dna. in all species except g. brevipalpis, the ribosomal genes were located on the long arm of autosome l1. the y chromosomes of g. pallidipes and g. p. palpalis showed additional hybridization signals. supernumer ... | 1997 | 9244454 |
| identification of the sex-determining region of the ceratitis capitata y chromosome by deletion mapping. | in the medfly ceratitis capitata, the y chromosome is responsible for determining the male sex. we have mapped the region containing the relevant factor through the analysis of y-autosome translocations using fluorescence in situ hybridization with two different probes. one probe, the clone py114, contains repetitive, y-specific dna sequences from c. capitata, while the second clone, pdh2-h8, consists of ribosomal dna sequences from drosophila hydei. clone py114 labeled most of the long arm and ... | 1996 | 8889534 |
| electron microscopic band-interband pattern of polytene chromosomes in drosophila nasuta albomicans. 2. salivary gland chromosome 2l. | the band-interband pattern (division 28-52) of salivary gland chromosome 2l in drosophila nasuta albomicans was studied by light (lm) and electron microscopy (em) using squash preparations and surface-spread polytene (ssp) chromosome preparations, respectively. lm and em maps were complied. based on the digitized em patterns of five homologous ssp chromosomes a computerized em chromosome map was plotted. the em pattern analysis showed a total number of 479 chromosome bands with an almost 83% inc ... | 1991 | 1807929 |
| phylogeny and molecular evolution of the drosophila hydei subgroup (drosophila repleta group) inferred from the xanthine dehydrogenase gene. | the hydei subgroup (drosophila repleta group) consists of seven species divided into two complexes: bifurca and hydei, whose phylogenetic relationships are not well understood. to evaluate the molecular phylogeny of this subgroup, we analyzed 2085 bp of coding sequence of the xanthine dehydrogenase gene in six available species of the hydei subgroup, with drosophila buzzatii and drosophila mulleri as an outgroup. for phylogenetic reconstruction we adopted a maximum-likelihood framework, based on ... | 2005 | 15935705 |
| discrimination of related transcribed and non-transcribed repetitive dna sequences from the y chromosomes of drosophila hydei and drosophila eohydei. | the short arm of the y chromosome of drosophila hydei carries a single male fertility gene, gene q, which forms the lampbrush loop pair nooses. conflicting observations have been reported concerning the identity of the repetitive dna sequences that are transcribed in this loop pair. it has been claimed by other investigators that the loop transcripts contain repeats of two distinct, but related families of y-specific repetitive dna sequences, ay1 and ysi. we reinvestigated this issue, using as p ... | 1994 | 8190071 |
| identification of optimal feedback control rules from micro-quadrotor and insect flight trajectories. | this paper presents "optimal identification," a framework for using experimental data to identify the optimality conditions associated with the feedback control law implemented in the measurements. the technique compares closed loop trajectory measurements against a reduced order model of the open loop dynamics, and uses linear matrix inequalities to solve an inverse optimal control problem as a convex optimization that estimates the controller optimality conditions. in this study, the optimal i ... | 2018 | 29299686 |
| experimental evolution of infectious behaviour in a facultative ectoparasite. | parasitic lifestyles have evolved many times in animals, but how such life-history strategies evolved from free-living ancestors remains a great puzzle. transitional symbiotic strategies, such as facultative parasitism, are hypothesized evolutionary stepping stones towards obligate parasitism. however, to consider this hypothesis, heritable genetic variation in infectious behaviour of transitional symbiotic strategies must exist. in this study, we experimentally evolved infectivity and estimated ... | 2017 | 29282821 |
| colour polymorphic lures exploit innate preferences for spectral versus luminance cues in dipteran prey. | theory predicts that colour polymorphism may be favored by variation in the visual context under which signals are perceived. the context encompasses all environmental determinants of light availability and propagation, but also the dynamics of perception in receivers. color vision involves the neural separation of information into spectral versus luminance channels, which often differentially guide specific tasks. here we explicitly tested whether this discrete perceptual basis contributes to t ... | 2017 | 28806928 |
| mitey costly: energetic costs of parasite avoidance and infection. | parasites reduce host fitness via perturbations to host energy allocation, growth, survival, and reproduction. here, we investigate the independent effects of parasite exposure and infection on host metabolic rate. our study focuses on drosophila hydei and a naturally occurring ectoparasitic mite, macrocheles muscaedomesticae. we use flow-through respirometry to measure the metabolic rate of flies during the period of exposure (preinfection) and during mite attachment. flies were exposed to mite ... | 2017 | 28414262 |
| separating multiple sources of variation on heat resistance in drosophila hydei. | while numerous insect studies have demonstrated the effects environmental conditions, genetic variation and other factors have on thermal resistance, often showing patterns consistent with adaptive plasticity and local adaptation, few experiments have considered the effects of multiple factors simultaneously. here however, we have investigated the impact of sex, rearing conditions, hardening, population, and laboratory rearing period on adult heat resistance in stocks of drosophila hydei, a cosm ... | 2017 | 27816712 |
| pollination of specklinia by nectar-feeding drosophila: the first reported case of a deceptive syndrome employing aggregation pheromones in orchidaceae. | the first documented observation of pollination in pleurothallidinae was that of endrés, who noticed that the 'viscid sepals' of specklinia endotrachys were visited by a 'small fly'. chase would later identify the visiting flies as being members of the genus drosophila. this study documents and describes how species of the s. endotrachys complex are pollinated by different drosophila species. | 2015 | 26071932 |
| rapid spread of the defensive endosymbiont spiroplasma in drosophila hydei under high parasitoid wasp pressure. | maternally transmitted endosymbionts of insects are ubiquitous in nature and play diverse roles in the ecology and evolution of their hosts. to persist in host lineages, many symbionts manipulate host reproduction to their advantage (e.g. cytoplasmic incompatibility and male-killing), or confer fitness benefits to their hosts (e.g. metabolic provisioning and defense against natural enemies). recent studies suggest that strains of the bacterial genus spiroplasma protect their host (flies in the g ... | 2015 | 25764546 |
| body saccades of drosophila consist of stereotyped banked turns. | the flight pattern of many fly species consists of straight flight segments interspersed with rapid turns called body saccades, a strategy that is thought to minimize motion blur. we analyzed the body saccades of fruit flies (drosophila hydei), using high-speed 3d videography to track body and wing kinematics and a dynamically scaled robot to study the production of aerodynamic forces and moments. although the size, degree and speed of the saccades vary, the dynamics of the maneuver are remarkab ... | 2015 | 25657212 |
| dna damage in haemocytes and midgut gland cells of steatoda grossa (theridiidae) spiders exposed to food contaminated with cadmium. | the aim of this study was to assess the genotoxic effects of cd on haemocytes and midgut gland cells of web-building spiders, steatoda grossa (theridiidae), exposed to the metal under laboratory conditions. analyzes were conducted on adult females and males, fed for four weeks with cadmium-contaminated drosophila hydei flies, grown on a medium suplemented with 0.25 mm cdcl2. the comet assay, providing a quantitative measure of dna strand breaks, was used to evaluate the dna damage caused by the ... | 2015 | 25531832 |
| sex-specific divergence for body size and desiccation-related traits in drosophila hydei from the western himalayas. | sex-specific-differences are a widespread source of genetic variation in various drosophila species. in the present study, we have examined desiccation survival in males and females of drosophila hydei from colder and drier montane conditions of the western himalayas (altitudinal populations; 600-2202 m). in contrast with most other studies in drosophilids, d. hydei males exhibited comparatively higher desiccation resistance despite smaller body size compared to females. accordingly, we tested t ... | 2014 | 25045840 |