Publications

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the specificity of yolk protein uptake in cyclorrhaphan diptera is conserved through evolution.yolk proteins are transported from the hemolymph into the oocytes of insects during vitellogenesis by receptor-mediated endocytosis. since other hemolymph proteins, both native and foreign, are not accumulated in the oocyte, the process of uptake is selective for yolk proteins. peptide domains within the yolk proteins must therefore be involved in receptor recognition. with the long-term aim of identifying these domains and to open the possibility of understanding the molecular basis of receptor ...19921487828
mitochondria in the flight muscles of insects. iii. mitochondrial cytochrome c in relation to the aging and wing beat frequency of flies.1. in the present study a correlation has been sought between aging, flight muscle mitochrondria (sarcosomes), cytochrome c, and flight ability in the blowfly, phormia regina. 2. during the 1st week of adult life, individual sarcosomes increase in mass from 2.7 x 10(-7) microg. dry weight at the time of emergence, to 8.5 x 10(-7) microg. by the 7th day. during this period of growth, the number of sarcosomes per fly (6.7 x 10(8)) remains constant. when mature, the sarcosomes account for 32.6 per ...195613295550
diversity of epithelial morphogenesis during eggshell formation in drosophilids.the eggshells of drosophilid species provide a powerful model for studying the origins of morphological diversity. the dorsal appendages, or respiratory filaments, of these eggshells display a remarkable interspecies variation in number and shape, and the epithelial patterning underlying the formation of these structures is an area of active research. to extend the analysis of dorsal appendage formation to include morphogenesis, we developed an improved 3d image reconstruction approach. this app ...201525953345
nonfixed duplication containing the adh gene and a truncated form of the adhr gene in the drosophila funebris species group: different modes of evolution of adh relative to adhr in drosophila.the sequence of the genomic region that contains the adh and adhr genes of drosophila funebris was used to demonstrate that both genes are present in species of the funebris group. the sequence of this genomic region reveals a 2.9-kb tandem duplication which encompasses 1.6 kb of the 5' flanking region, the entire adh gene, and two thirds of the first exon of the adhr gene in d. funebris. this duplication is not fixed in this species since some strains do not carry the duplication. the adh dupli ...199910555275
divergence of the regulation of alpha-amylase activity in drosophila melanogaster, drosophila funebris, and drosophila saltans.the regulation of amylase activity in three drosophila species, d. melanogaster, d. funebris and d. saltans, was analyzed by measuring the specific activity levels in four dietary environments, cornmeal, glucose, 5% starch, and 10% starch, at three developmental stages, i.e., the third-instar larval, pupal, and 2-day-old adult stages. the developmental profiles of amylase activity for the three drosophila species showed that the level of activity was high at the larval and adult stages but subst ...199910429831
evidence for horizontal transmission of the mobile element jockey between distant drosophila species.we addressed the possibility of the horizontal transfer of long interspersed element (line)-like mobile elements by studying the distribution of the drosophila melanogaster line-like element jockey in different drosophila species. outside the d. melanogaster group jockey was detected only in the distantly related species drosophila funebris. cloning and sequencing of this element from d. funebris revealed the existence of the two open reading frames highly similar to those of jockey from d. mela ...19901701254
effect of ethanol and isopropanol on the activity of alcohol dehydrogenase, viability and life-span in drosophila melanogaster and drosophila funebris.the effects caused by the addition of 2 alcohols to the culture medium of 2 species of drosophila, d. melanogaster and d. funebris, are compared. ethanol at 1% concentration causes slight and tolerable changes in both species. 1% isopropanol is lethal in 1 species and causes drastic changes in the other.19806772467
ectopic pairing and evolution of 5s ribosomal rna genes in the chromosomes of drosophila funebris.5s ribosomal rna from drosophila melanogaster labeled with 125i was used to locate the 5s rrna genes in chromosomes of d. funebris by means of in situ hybridization. silver grains were observed at three distinct sites, one of which was a recognized reverse repeat. only one half of the reverse repeat, however, hybridizes with 5s rrna and the significance of this phenomenon is discussed. a case of ectopic pairing between two different 5s sites in the genome is reported, and the significance of ect ...1976824107
binding site number variation and high-affinity binding consensus of myb-sant-like transcription factor adf-1 in drosophilidae.there is a growing interest in the evolution of transcription factor binding sites and corresponding functional change of transcriptional regulation. in this context, we have examined the structural changes of the adf-1 binding sites at the adh promoters of drosophila funebris and d. virilis. we detected an expanded footprinted region in d. funebris that contains various adjacent binding sites with different binding affinities. adf-1 was described to direct sequence-specific dna binding to sites ...201020542916
genetics analysis of larval foraging behavior in drosophila funebris.to understand the genetics and evolution of foraging in larvae of drosophila funebris, we examined two strains reared at different breeding sites in the wild. larvae of the til-til strain breed in necrotic cactus tissue, while those of the pelequén strain rear in necrotic prickly pear cladodes. we measured feeding, locomotion, turning behavior, and latency of d. funebris. til-til and pelequén larvae, at 8 days of age show very similar rates in all behaviors. crosses between til-til and pelequén ...200818661224
evolutionary change in the structure of the regulatory region that drives tissue and temporally regulated expression of alcohol dehydrogenase gene in drosophila funebris.the adh locus of drosophilidae is organized as a single gene transcribed from two spatially and temporally regulated promoters except in species of the repleta group, which have two single promoter genes. here we show that in drosophila funebris the adh gene is transcribed from a single promoter, in both larva and adult, with qualitative and quantitative species specific-differences in tissue distribution. the gene is expressed in larval fat body but in other tissues such as gastric caeca, midgu ...200111437915
extinction due to evolution of a competitor.when drosophila simulans and drosophila funebris were cultured together in population cages over many generations, there was a prolonged period of apparently stable coexistence followed by a rapid exclusion of d. funebris. as both species maintained large population sizes in monocultures it follows that the extinctions of d. funebris in the mixed-species cultures must have been caused by d. simulans. the time to extinction of d. funebris ranged from 26 weeks in one cage, to between 40 and 48 wee ...199128567883
a male accessory gland peptide with protease inhibitory activity in drosophila funebris.male accessory glands of drosophila funebris synthesize and secrete a peptide that shows a protease-inhibiting activity. amino acid sequencing of the purified peptide revealed that the peptide consists of 63 amino acid residues. it is a serine protease inhibitor belonging to the pancreatic trypsin inhibitor (kunitz) family. the inhibitory function and the kinetic characteristics of the inhibition have been examined with various substrates. the peptide possibly plays a role as an acrosin inhibito ...19892722874
residual influences on fecundity in drosophilid species.a residual influence of males and females on oviposition has been examined in 7 drosophilids. there was evidence for oviposition deterrence in drosophila funebris, with males as well as females producing the inhibitory effect. in contrast, male residues stimulated oviposition in zaprionus tuberculatus. male residues also stimulated oviposition and appeared to serve as an aggregation cue in d. melanogaster.19873817108
alcohol dehydrogenase from drosophila funebris and drosophila immigrans: molecular and evolutionary aspects.alcohol dehydrogenase from drosophila funebris and d. immigrans is evident at all developmental stages. the highest activity level appears in third-instar larvae and declines to a lower level at all later stages of development. both species are monomorphic. the enzyme is a dimer consisting of two identical subunits with molecular weight 27,600. the pi values are 8.6 for d. funebris and 9.02 for d. immigrans. the optimum ph is 8.6 and 8.7 for d. funebris and d. immigrans, respectively. the km val ...19846393950
the amino-acid sequence of a peptide (ps-1) from drosophila funebris: a paragonial peptide from males which reduces the receptivity of the female.the substance, ps-1, produced in the paragonial gland of adult male drosophila funebris influences the mating behavior of virgin female flies after injection. the substance was isolated and characterized as a 27-residue peptide. the complete amino acid sequence was determined by manual sequence analysis of tryptic peptides, automated edman degradation, and carboxypeptidase a digestion. the sequence is asp-val/leu-pro-ser-ala-asn-ala-asn-ala-asn-gln-arg-thr-ala-ala-ala-lys-pro-gln-ala-asn-ala-glu ...19751236144
biological effects of paragonial substances ps-1 and ps-2, in females of drosophila funebris. 19744436580
the isolation, partial characterization, and biosynthesis of the paragonial substances, ps-1 and ps-2 of drosophila funebris. 19744421531
[effect of temperature on chromosome polymorphism in drosophila funebris]. 19744837793
[effect of temperature on chromosomal polymorphism in drosophila funebris]. 19734807123
[comparison of the observed and anticipated distribution of genotypes in drosophila funebris 2d chromosome at 2 different temperature regimes]. 19734807119
electric characteristics of the cellular and nuclear membranes of the salivary gland cells of drosophila funebris larvae. 19714397895
studies on natural populations of drosophila xi. fitness components and competition between drosophila funebris and d. virilis. 197028562998
[existence of rest potentials in the cell nucleus of the salivary glands of drosophila funebris larvae]. 20035457906
wild population studies; drosophila funebris near manchester. 194818893750
inversion gradients and selection in ecological races of drosophila funebris. 201020297038
inversion gradients and natural selection in ecological races of drosophila funebris. 194617247218
structural chromosome variability in urban and rural populations of drosophila funebris. 201020982741
natural selection and chromosomal variability in populations of drosophila funebris. 194621025139
studies on the phenotypic manifestation of hereditary factors. i. on the phenotypic manifestation of the genovariation radius incompletus in drosophila funebris. 192717246520
a reverse genovariation in drosophila funebris. 192717246519
a parallel mutation in drosophila funebris. 191817818909
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