| molecular genetic variation in the centromeric region of the x chromosome in three drosophila ananassae populations. ii. the om(1d) locus. | restriction-site and sequence-length polymorphism in the om(1d) locus region on the x chromosome in drosophila ananassae was investigated for three natural populations (from burma, india, and brazil), by using hexanucleotide-recognizing restriction enzymes. the estimates of average heterozygosity per nucleotide (pi) were 0.0085, 0.0043, and 0.0004 for the burma, india, and brazil populations, respectively, and the average frequencies of insertions/deletions were 0.078, 0.054, and 0.007/chromosom ... | 1989 | 2577362 |
| evolution of mating isolation between populations of drosophila ananassae. | prezygotic mating isolation has been a major interest of evolutionary biologists during the past several decades because it is likely to represent one of the first stages in the transition from populations to species. mate discrimination is one of the most commonly measured forms of prezygotic isolation and appears to be relatively common among closely related species. in some cases, it has been used as a measure to distinguish populations from subspecies, races, and sister species, yet the infl ... | 2008 | 18466237 |
| bayesian inference of population size history from multiple loci. | effective population size (ne) is related to genetic variability and is a basic parameter in many models of population genetics. a number of methods for inferring current and past population sizes from genetic data have been developed since jfc kingman introduced the n-coalescent in 1982. here we present the extended bayesian skyline plot, a non-parametric bayesian markov chain monte carlo algorithm that extends a previous coalescent-based method in several ways, including the ability to analyze ... | 2008 | 18947398 |
| genetics of phototactic behavior in drosophila ananassae, a member of the melanogaster species group. | | 1998 | 106834 |
| the coevolutionary period of wolbachia pipientis infecting drosophila ananassae and its impact on the evolution of the host germline stem cell regulating genes. | the endosymbiotic bacteria wolbachia pipientis is known to infect a wide range of arthropod species yet less is known about the coevolutionary history it has with its hosts. evidence of highly identical w. pipientis strains in evolutionary divergent hosts suggests horizontal transfer between hosts. for example, drosophila ananassae is infected with a w. pipientis strain that is nearly identical in sequence to a strain that infects both d. simulans and d. suzukii, suggesting recent horizontal tra ... | 2014 | 24974378 |
| comparative gene expression analysis of dtg, a novel target gene of dpp signaling pathway in the early drosophila melanogaster embryo. | in the early drosophila melanogaster embryo, dpp, a secreted molecule that belongs to the tgf-β superfamily of growth factors, activates a set of downstream genes to subdivide the dorsal region into amnioserosa and dorsal epidermis. here, we examined the expression pattern and transcriptional regulation of dtg, a new target gene of dpp signaling pathway that is required for proper amnioserosa differentiation. we showed that the expression of dtg was controlled by dpp and characterized a 524-bp e ... | 2014 | 24321690 |
| extensive duplication of the wolbachia dna in chromosome four of drosophila ananassae. | lateral gene transfer (lgt) from bacterial wolbachia endosymbionts has been detected in ~20% of arthropod and nematode genome sequencing projects. many of these transfers are large and contain a substantial part of the wolbachia genome. | 2014 | 25496002 |
| formal relations between om mutants and their suppressors in drosophila ananassae. | optic morphology (om) mutants associated with insertions of the tom transposable element at each of three tested loci are neomorphs as defined by the phenotypic equivalence of +/+/om with +/om and of +/om/om with om/om. mutants behaving as suppressors of om mutants and mapping to at least six loci are recovered from the same source and in similar frequency as om mutants. the semidominant and nonpleiotropic suppressors at four of the six loci display defective eye phenes themselves, and the pheno ... | 2008 | 2852141 |
| experimental evidence for nutrition regulated stress resistance in drosophila ananassae. | the amount and quality of nutrients consumed by organisms have a strong impact on stress resistance, life-history traits and reproduction. the balance between energy acquisition and expenditure is crucial to the survival and reproductive success of animals. the ability of organisms to adjust their development, physiology or behavior in response to environmental conditions, called phenotypic plasticity, is a defining property of life. one of the most familiar and important examples of phenotypic ... | 2012 | 23049693 |
| population genetics of drosophila ananassae. | drosophila ananassae doleschall is a cosmopolitan and domestic species. it occupies a unique status among drosophila species due to certain peculiarities in its genetic behaviour and is of common occurrence in india. quantitative genetics of sexual and non-sexual traits provided evidence for genetic control of these traits. d. ananassae exhibits high level of chromosomal polymorphism in its natural populations. indian natural populations of d. ananassae show geographic differentiation of inversi ... | 2008 | 19061531 |
| population genetics of drosophila ananassae: genetic differentiation among indian natural populations at the level of inversion polymorphism. | the present study, which is one of the longest temporal (two decades) and largest spatial (different parts of india covered) investigations on inversion polymorphism in natural populations of d. ananassae, was undertaken to understand the dynamics of inversion polymorphism in a broad and comprehensive manner. forty-five natural populations from different ecogeographic regions of the country (covering the regions from kashmir to kanniyakumari and gujarat to nagaland) were analysed for chromosomal ... | 2007 | 18208624 |
| the genetic structure of drosophila ananassae populations from asia, australia and samoa. | information about genetic structure and historical demography of natural populations is central to understanding how natural selection changes genomes. drosophila ananassae is a widespread species occurring in geographically isolated or partially isolated populations and provides a unique opportunity to investigate population structure and molecular variation. we assayed microsatellite repeat-length variation among 13 populations of d. ananassae to assess the level of structure among the populat ... | 2007 | 17237518 |
| effect of temperature on behavioural isolation: a study with drosophila ananassae populations. | experiments were conducted to study sexual isolation among two natural populations of drosophila ananassae maintained at 18 degrees c and 24 degrees c for 12 generations in the laboratory to see the effect of this environmental variable on behavioural isolation. multiple choice technique was used and matings were observed directly in elens wattiaux mating chamber. results showed sexual isolation among strains that were maintained at different temperatures, indicating that temperature may have af ... | 2012 | 22803327 |
| crossing over does occur in males of drosophila ananassae from natural populations. | spontaneous crossing over in males of drosophila ananassae has been well demonstrated using f(1) individuals from crosses between marker stocks and wild type strains. however, the question of its occurrence in males from natural populations remained open. here we present the cytological evidence that crossing over does occur in males of d. ananassae from two brazilian populations, sampled nearly 21 years apart, and in two recently sampled populations, one from indonesia and one from okinawa, jap ... | 2012 | 22788379 |
| serendipitous discovery of wolbachia genomes in multiple drosophila species. | the trace archive is a repository for the raw, unanalyzed data generated by large-scale genome sequencing projects. the existence of this data offers scientists the possibility of discovering additional genomic sequences beyond those originally sequenced. in particular, if the source dna for a sequencing project came from a species that was colonized by another organism, then the project may yield substantial amounts of genomic dna, including near-complete genomes, from the symbiotic or parasiti ... | 2005 | 15774024 |
| disruptive selection for sternopleural bristle phenotypes in drosophila ananassae. | disruptive selection is potentially critical in maintaining variation and initiating speciation and plays an important role in the organization of genetic variability in natural populations. it occurs when extreme phenotypes have a fitness advantage over intermediate phenotypes. disruptive selection for high and low numbers of sternopleural bristles in drosophila ananassae was applied for 12 generations to test its effect in induction of behavioural isolation. pattern of mating between flies of ... | 2011 | 21970458 |
| a comparison of frost expression among species and life stages of drosophila. | frost (fst) is a gene associated with cold exposure in drosophila melanogaster. we used real-time pcr to assess whether cold exposure induces expression of fst in 10 different life stages of d. melanogaster, and adults of seven other drosophila species. we exposed groups of individuals to 0 °c (2 h), followed by 1 h recovery (22 °c). frost was significantly upregulated in response to cold in eggs, third instar larvae, and 2- and 5-day-old male and female adults in d. melanogaster. life stages in ... | 2012 | 21955087 |
| inferring the population structure and demography of drosophila ananassae from multilocus data. | inferring the origin, population structure, and demographic history of a species is a major objective of population genetics. although many organisms have been analyzed, the genetic structures of subdivided populations are not well understood. here we analyze drosophila ananassae, a highly substructured, cosmopolitan, and human-commensal species distributed in the tropical, subtropical, and mildly temperate regions of the world. we adopt a multilocus approach (with 10 neutral loci) using 16 popu ... | 2004 | 15611168 |
| joint effects of natural selection and recombination on gene flow between drosophila ananassae populations. | we estimated dna sequence variation in a 5.7-kb fragment of the furrowed (fw) gene region within and between four populations of drosophila ananassae; fw is located in a chromosomal region of very low recombination. we analyzed gene flow between these four populations along a latitudinal transect on the indian subcontinent: two populations from southern, subtropical areas (hyderabad, india, and sri lanka) and two from more temperate zones in the north (nepal and burma). furthermore, we compared ... | 2000 | 10880480 |
| drosophila ananassae: a good model species for genetical, behavioural and evolutionary studies. | drosophila ananassae, a cosmopolitan and domestic species, was first described by doleschall in 1858 from indonesia. during 1930s, cytological and genetical investigations in d. ananassae were initiated in japan and usa which showed that it is a genetically unique species. since then a large number of studies have been carried out by researchers in japan, u.s.a., india, france and germany in this genetically unique species. present review briefly summarizes the work done on genetical, behavioura ... | 2010 | 20726331 |
| population and behaviour genetics of drosophila ananassae. | drosophila ananassae is a cosmopolitan and domestic species. it occupies a unique status among the drosophila species due to certain peculiarities in its genetic behaviour. the most unusual feature of this species is spontaneous male recombination in appreciable frequency. the present review summarizes the work done on population and behaviour genetics of d. ananassae from india. population dynamics of three cosmopolitan inversions has been studied in indian populations of d. ananassae and it is ... | 1996 | 9081860 |
| robust target gene discovery through transcriptome perturbations and genome-wide enhancer predictions in drosophila uncovers a regulatory basis for sensory specification. | a comprehensive systems-level understanding of developmental programs requires the mapping of the underlying gene regulatory networks. while significant progress has been made in mapping a few such networks, almost all gene regulatory networks underlying cell-fate specification remain unknown and their discovery is significantly hampered by the paucity of generalized, in vivo validated tools of target gene and functional enhancer discovery. we combined genetic transcriptome perturbations and com ... | 2010 | 20668662 |
| inversion polymorphism and extra bristles in indian natural populations of drosophila ananassae: joint variation. | five indian natural populations of drosophila ananassae were analysed for chromosome inversions and the presence of individuals with extra scutellar bristles in the f1 progeny of isofemale lines initiated from naturally impregnated females. three commonly occurring inversions were found in these populations with varying frequencies as was the number of individuals with extra bristles (e.b.). female individuals were more often found to carry extra scutellar bristles than were males. this result r ... | 1994 | 7989220 |
| evolutionary relationships in the drosophila ananassae species cluster based on introns of multiple nuclear loci. | the drosophila ananassae species cluster includes d. ananassae, d. pallidosa, d. parapallidosa, and the cryptic species "pallidosa-like", "pallidosa-like wau" and "papuensis-like" some of the taxa are sympatric in the south pacific, papua new guinea, and southeast asia, and gene flow between different taxa has been suspected for a handful of genes. in the present analysis, we examined dna sequences of introns in four loci: alpha actinin (actn) on xl, white (w) on xr, cg7785 on 2l, and zinc ion t ... | 2010 | 20377348 |
| recent horizontal transfer of a mariner transposable element among and between diptera and neuroptera. | transposable elements of the mariner family are widespread among insects and other invertebrates, and initial analyses of their relationships indicated frequent occurrence of horizontal transfers between hosts. a specific pcr assay was used to screen for additional members of the irritans subfamily of mariners in more than 400 arthropod species. phylogenetic analysis of cloned pcr fragments indicated that relatively recent horizontal transfers had occurred into the lineages of a fruit fly drosop ... | 1995 | 7476131 |
| molecular evolution of sex-biased genes in the drosophila ananassae subgroup. | genes with sex-biased expression often show rapid molecular evolution between species. previous population genetic and comparative genomic studies of drosophila melanogaster and d. simulans revealed that male-biased genes have especially high rates of adaptive evolution. to test if this is also the case for other lineages within the melanogaster group, we investigated gene expression in d. ananassae, a species that occurs in structured populations in tropical and subtropical regions. we used cus ... | 2009 | 20015359 |
| insights into differential activity patterns of drosophilids under semi-natural conditions. | we showed recently that drosophila ananassae, a closely related and sympatric species of the commonly studied fruitfly d. melanogaster, shows distinctly deviant patterns in circadian activity/rest rhythm from the latter under a variety of laboratory conditions. to examine whether such differences extend to more natural conditions where a variety of time cues and similar environmental pressures might force different species to adopt similar temporal patterns, we examined these two species under s ... | 2013 | 24143027 |
| variations in morphological and life-history traits under extreme temperatures in drosophila ananassae. | using half-sib analysis, we analysed the consequences of extreme rearing temperatures on genetic and phenotypic variations in the morphological and life-history traits of drosophila ananassae. paternal half-sib covariance contains a relatively small proportion of the epistatic variance and lacks the dominance variance and variance due to maternal effect, which provides more reliable estimates of additive genetic variance. experiments were performed on a mass culture population of d. ananassae co ... | 2009 | 19550042 |
| effect of mutations on developmental stability and canalization in morphological traits in drosophila ananassae. | the present study was designed to determine the effects of visible mutations of large effect on developmental stability and canalization in different morphological traits, namely, sternopleural bristle number, wing length, wing to thorax ratio, ovariole number, and sex comb tooth number (sctn) in drosophila ananassae. we have compared the mean trait size, fluctuating asymmetry (fa) (as an index of developmental stability), and morphological variation (as an index of canalization) of different mu ... | 2008 | 18477590 |
| population genomics of infectious and integrated wolbachia pipientis genomes in drosophila ananassae. | coevolution between drosophila and its endosymbiont wolbachia pipientis has many intriguing aspects. for example, drosophila ananassae hosts two forms of w. pipientis genomes: one being the infectious bacterial genome and the other integrated into the host nuclear genome. here, we characterize the infectious and integrated genomes of w. pipientis infecting d. ananassae (wana), by genome sequencing 15 strains of d. ananassae that have either the infectious or integrated wana genomes. results indi ... | 2015 | 26254486 |
| patterns of amino acid evolution in the drosophila ananassae chimeric gene, siren, parallel those of other adh-derived chimeras. | siren1 and siren2 are novel alcohol dehydrogenase (adh)-derived chimeric genes in the drosophila bipectinata complex. d. ananassae, however, harbors a single homolog of these genes. like other adh-derived chimeric genes, siren evolved adaptively shortly after it was formed. these changes likely shifted the catalytic activity of siren. | 2008 | 18780749 |
| genomic resources for multiple species in the drosophila ananassae species group. | the development of genomic resources in non-model taxa is essential for understanding the genetic basis of biological diversity. although the genomes of many drosophila species have been sequenced, most of the phenotypic diversity in this genus remains to be explored. to facilitate the genetic analysis of interspecific and intraspecific variation, we have generated new genomic resources for seven species and subspecies in the d. ananassae species subgroup. we have generated large amounts of tran ... | 2017 | 23639891 |
| invasion and adaptation of a warm-adapted species to montane localities: effect of acclimation potential. | drosophila ananassae has successfully invaded the cold and dry montane localities of the western himalayas in recent years. the ability of this desiccation- and cold-sensitive tropical species to evolve in response to seasonal changes in montane localities is largely unknown. here, we investigated how this sensitive species adapt to seasonally varying environmental conditions that are lethal to its survival. we observed change in the frequency of dark and light morphs of d. ananassae in five mid ... | 2013 | 23348937 |
| genetic analysis of female mating recognition between drosophila ananassae and drosophila pallidosa: application of interspecific mosaic genome lines. | drosophila ananassae and drosophila pallidosa are closely related species that can produce viable and fertile hybrids of both sexes, although strong sexual isolation exists between the two species. females are thought to discriminate conspecific from heterospecific males based on their courtship songs. the genetic basis of female discrimination behavior was analyzed using isogenic females from interspecific mosaic genome lines that carry homozygous recombinant chromosomes. multiple regression an ... | 2008 | 17768595 |
| sympatric drosophilid species melanogaster and ananassae differ in temporal patterns of activity. | the fruit fly drosophila melanogaster has long served as a model system for circadian rhythm research. various aspects of its genetic, molecular, and circuit-level properties are the subject of investigation, based on which several circadian behaviors and their neuronal controls have been unraveled. in an attempt to address the question of functional significance of circadian organization using a comparative approach, we studied activity/rest rhythm of wild-caught d. melanogaster (dm) and its cl ... | 2012 | 23010659 |
| adaptation to larval crowding in drosophila ananassae leads to the evolution of population stability. | density-dependent selection is expected to lead to population stability, especially if r and k tradeoff. yet, there is no empirical evidence of adaptation to crowding leading to the evolution of stability. we show that populations of drosophila ananassae selected for adaptation to larval crowding have higher k and lower r, and evolve greater stability than controls. we also show that increased population growth rates at high density can enhance stability, even in the absence of a decrease in r, ... | 2012 | 22837839 |
| the precopulatory function of male genital spines in drosophila ananassae[doleschall] (diptera: drosophilidae) revealed by laser surgery. | that male genital morphology evolves via postcopulatory sexual selection is a widely held view. in contrast, the precopulatory sexual selection hypothesis for genital evolution has received less attention. here, we test the hypothesis that male genital spines of drosophila ananassae promote competitive male copulation success. using laser surgery to manipulate trait size, we demonstrate that incremental reductions of spine length progressively reduce male copulation success: males without spines ... | 2012 | 22834760 |
| efficient subtraction of insect rrna prior to transcriptome analysis of wolbachia-drosophila lateral gene transfer. | numerous methods exist for enriching bacterial or mammalian mrna prior to transcriptome experiments. yet there persists a need for methods to enrich for mrna in non-mammalian animal systems. for example, insects contain many important and interesting obligate intracellular bacteria, including endosymbionts and vector-borne pathogens. such obligate intracellular bacteria are difficult to study by traditional methods. therefore, genomics has greatly increased our understanding of these bacteria. e ... | 2012 | 22583543 |
| origin of sexual isolation in drosophila ananassae due to founder effects. | the origin of sexual isolation is the central event in the evolution of biological species and plays a key role in maintaining biological diversity. three mass culture stocks of d. ananassae originating from different geographic localities showing no isolation with each other were subjected to different degrees of bottlenecks i.e. one pair, five pairs and ten pairs. these drift lines were passed through flush-crash cycle at every generation with same initial number of founders, and maintained fo ... | 2011 | 21626152 |
| effect of chromosome arrangements on mate recognition system leading to behavioral isolation in drosophila ananassae. | the mechanisms of speciation that appear in the early stages of reproductive isolation has been of recent interest to evolutionary biologists. experiments were conducted to study behavioral isolation between karyotypically different homozygous strains derived from natural populations of drosophila ananassae. three mass cultures stocks established from flies collected from natural populations were employed and homozygous stocks (st/st and al/al) were made through selection for homozygosity. by em ... | 2011 | 21234646 |
| establishing interspecific mosaic genome lines between drosophila ananassae and drosophila pallidosa by means of parthenogenesis. | strong sexual isolation exists between the closely related species drosophila ananassae and d. pallidosa, but there is no obvious post-mating isolation; both sexes of the hybrids and their descendants appear to be completely viable and fertile. strains exhibiting parthenogenesis have been derived from wild populations of both species. we intercrossed such strains and established iso-female lines after the second generation of parthenogenesis. these lines are clones, carrying homozygous chromosom ... | 2006 | 17014740 |
| resistance to environmental stress in drosophila ananassae: latitudinal variation and adaptation among populations. | geographical variation in traits related to fitness is often the result of adaptive evolution. stress resistance traits in drosophila often show clinal variation, suggesting that selection affects resistance traits either directly or indirectly. multiple stress resistance traits were investigated in 45 natural populations of drosophila ananassae collected from all over india. there was significant positive correlation between starvation resistance and lipid content. significant negative correlat ... | 2010 | 20695963 |
| conservation of male-specific expression of novel phosphoprotein phosphatases in drosophila. | in the genome of drosophila melanogaster, there are 19 phosphoprotein phosphatase (ppp) catalytic subunit coding genes. seven of the novel members of the gene family turned out to be drosophila-specific. the expression and evolution of these genes was investigated in the present study. cg11597 is a recently evolved gene that is expressed during all stages of morphogenesis in d. melanogaster. in contrast, the transcription of ppd5, ppd6, pp1-y1, and pp1-y2 genes is restricted to the pupa and imag ... | 2010 | 20632030 |
| simple synthesis of the major sex pheromone components of drosophila ananassae and d. pallidosa. | the major sex pheromone components of drosophila ananassae and d. pallidosa, (z,z)-5,25-hentriacontadiene and (z,z)-5,27-tritriacontadiene, respectively, were synthesized by using the wittig olefination and sulfone coupling reactions as the c-c bond-forming steps. | 2005 | 16116297 |
| what the loss of the hormone neuroparsin in the melanogaster subgroup of drosophila can tell us about its function. | the twelve sequenced drosophila genomes show the vast majority of neurohormone and neuropeptide genes to be very well conserved. nonetheless, the gene encoding the hormone neuroparsin has undergone significant evolution. although drosophila melanogaster has one of the best known genomes, no neuroparsin gene can be detected in either the assembled genome or any of the individual sequencing traces. this gene is similarly absent from the genomes of other species in the melanogaster subgroup, even t ... | 2010 | 20226240 |
| evolution in the drosophila ananassae species subgroup. | drosophila ananassae and its relatives have many advantages as a model of genetic differentiation and speciation. in this report, we examine evolutionary relationships in the ananassae species subgroup using a multi-locus molecular data set, karyotypes, meiotic chromosome configuration, chromosomal inversions, morphological traits, and patterns of reproductive isolation. we describe several new taxa that are the closest known relatives of d. ananassae. analysis of y-chromosomal and mitochondrial ... | 2010 | 19377294 |
| is genome size influenced by colonization of new environments in dipteran species? | genome size differences are usually attributed to the amplification and deletion of various repeated dna sequences, including transposable elements (tes). because environmental changes may promote modifications in the amount of these repeated sequences, it has been postulated that when a species colonizes new environments this could be followed by an increase in its genome size. we tested this hypothesis by estimating the genome size of geographically distinct populations of drosophila ananassae ... | 2005 | 15723678 |
| divergence of water balance mechanisms and acclimation potential in body color morphs of drosophila ananassae. | drosophila ananassae is a desiccation sensitive species, but the physiological basis of its abundance in the drier subtropical areas is largely unknown. we tested the hypothesis whether body color morphs of d. ananassae differ in the mechanistic basis of water conservation as well as desiccation acclimation potential, consistent with their distribution under dry or wet habitats. we observed reduced rate of water loss consistent with the greater desiccation potential of dark morph as compared wit ... | 2014 | 24167067 |
| a novel dataset for identifying sex-biased genes in drosophila. | phenotypic differences between males and females of sexually dimorphic species are caused in large part by differences in gene expression between the sexes, most of which occurs in the gonads. to accurately identify genes differentially expressed between males and females in drosophila, we sequenced the testis and ovary transcriptomes of d. yakuba, d. pseudoobscura, and d. ananassae and used them to identify sex-biased genes in the latter two species. we highlight the increased sensitivity and i ... | 2014 | 25031657 |
| evaluating the post-copulatory sexual selection hypothesis for genital evolution reveals evidence for pleiotropic harm exerted by the male genital spines of drosophila ananassae. | the contemporary explanation for the rapid evolutionary diversification of animal genitalia is that such traits evolve by post-copulatory sexual selection. here, we test the hypothesis that the male genital spines of drosophila ananassae play an adaptive role in post-copulatory sexual selection. whereas previous work on two drosophila species shows that these spines function in precopulatory sexual selection to initiate genital coupling and promote male competitive copulation success, further re ... | 2014 | 25403358 |
| species and genetic diversity in the genus drosophila inhabiting the indian subcontinent. | biodiversity is the sum total of all living things on the earth with particular reference to the profound variety in structure,function and genetic constitution. it includes both number and frequency of species or genes in a given assemblage and the variety of resulting ecosystems in a region. it is usually considered at three different levels: genetic, species and ecological diversities. genus drosophila belongs to the family drosophilidae (class insecta, order diptera), characterized by rich s ... | 2015 | 26174688 |
| status of research on drosophila ananassae at global level. | drosophila, a dipteran insect, has been found to be the best biological model for different kinds of studies. d melanogaster was first described by meigen in 1830 , is most extensively studied species of the genus drosophila and a number of investigations employing this species have been documented in areas such as genetics, behaviour, evolution, development, molecular biology, ecology, population biology, etc. besides d. melanogaster, a number of other species of the genus drosophila have also ... | 2015 | 26690536 |
| isolation and characterization of dinucleotide repeat microsatellites in drosophila ananassae. | drosophila ananassae is a cosmopolitan species with a geographic range throughout most of the tropical and subtropical regions of the world. previous studies of dna sequence polymorphism in three genes has shown evidence of selection affecting broad expanses of the genome in regions with low rates of recombination in geographically local populations in and around india. the studies suggest that extensive physical and genetic maps based on molecular markers, and detailed studies of population str ... | 2004 | 15125063 |
| male recombination in brazilian populations of drosophila ananassae. | with few exceptions, spontaneous crossing over does not normally occur in male drosophila. drosophila ananassae males show considerable amounts of crossing over. in wild males of d. ananassae from asian (2008) and brazilian populations (1986 and 2007) variable frequencies of meiotic crossing over, estimated from chiasmata counts, suggested the existence of factors controlling male crossing over in these populations. to corroborate for such prediction, we present data on spontaneous recombination ... | 2016 | 27314475 |
| rare male mating advantage for inversion karyotype in drosophila ananassae. | these experiments were conducted to study frequency-dependent sexual selection on the alpha inversion in the left arm of the second chromosome (2l) in drosophila ananassae. two different strains, st/st-standard gene arrangement and al/al-alpha inversion in 2l were used. female-choice experiments were carried out in five different male ratios. two different methods of observation have been employed, one in which copulating pairs are aspirated out and the other, in which pairs are not removed. res ... | 2004 | 14990872 |
| hsp27 gene in drosophila ananassae subgroup was split by a recently acquired intron. | in drosophila, heat shock protein 27 (hsp27) is a critical single-copy intron-free nuclear gene involved in the defense response against fungi and bacteria, and is a regulator of adult lifespan. in the present study, 33 homologous hsp27 nucleotide sequences from different drosophila species were amplified by pcr and reverse transcription pcr, and the phylogenetic relationships were analysed using neighbour-joining, maximum-likelihood and bayesian methods. the phylogenetic topologies from analysi ... | 2016 | 27350667 |
| evolutionary history and mode of the amylase multigene family in drosophila. | previous studies indicate that the tandemly repeated members of the amylase (amy) gene family evolved in a concerted manner in the melanogaster subgroup and in some other species. in this paper, we analyzed all of the 49 active and complete amy gene sequences in drosophila, mostly from subgenus sophophora. phylogenetic analysis indicated that the two types of diverged amy genes in the drosophila montium subgroup and drosophila ananassae, which are located in distant chromosomal regions from each ... | 2003 | 14745539 |
| adaptation to larval crowding in drosophila ananassae and drosophila nasuta nasuta: increased larval competitive ability without increased larval feeding rate. | the standard view of adaptation to larval crowding in fruitflies, built on results from 25 years of multiple experimental evolution studies on drosophila melanogaster, was that enhanced competitive ability evolves primarily through increased larval feeding and foraging rate, and increased larval tolerance to nitrogenous wastes, at the cost of efficiency of food conversion to biomass. these results were at odds from the predictions of classical k-selection theory, notably the expectation that sel ... | 2016 | 27350686 |
| a nested alpha-amylase gene in drosophila ananassae. | the amylase gene family of drosophila ananassae consists in seven copies, scattered on several chromosomal arms. we have evidenced that a member of the family, amy35, lies within an intron of a gene homologous to the cg14696 gene of d. melanogaster. this nested arrangement seems restricted to the d. ananassae subgroup. the nested and the nest genes are encoded on opposite strands. both are actively transcribed in the midgut at the same time, raising the possibility of interference between their ... | 2003 | 14629045 |
| highly conserved drosophila ananassae timeless gene functions as a clock component in drosophila melanogaster. | the behavior and physiology of drosophila are subject to rhythms that are controlled by the circadian clock genes, period, timeless, clock and cycle, all of which are thought to participate in central pacemaker control. the molecular mechanism of rhythm in drosophila has been studied in detail. however, rhythm and clock genes have mostly been analyzed in drosophila melanogaster. to confirm whether the tim gene exists and works as a clock component in other drosophila species, we cloned a tim hom ... | 2003 | 12706901 |
| recent and long-term selection across synonymous sites in drosophila ananassae. | in drosophila, many studies have examined the short- or long-term evolution occurring across synonymous sites. few, however, have examined both the recent and long-term evolution to gain a complete view of this selection. here we have analyzed drosophila ananassae dna polymorphism and divergence data using several different methods, and have identified evidence of positive selection favoring preferred codons in both recent and long-term evolutionary time scale. further in d. ananassae, the stren ... | 2016 | 27481397 |
| temperature dependent eclosion rhythmicity in the high altitude himalayan strains of drosophila ananassae. | the circadian pacemaker controlling the eclosion rhythm of the high altitude himalayan strains of drosophila ananassae captured at badrinath (5123 m) required ambient temperature at 21 degrees c for the entrainment and free-running processes. at this temperature, their eclosion rhythms entrained to 12h light, 12h dark (ld 12:12) cycles and free-ran when transferred from constant light (ll) to constant darkness (dd) or upon transfer to constant temperature at 21 degrees c following entrainment to ... | 2002 | 12511025 |
| a natural population derived from species hybridizationin the drosophila ananassae species complexon penang island, malaysia. | we surveyed natural population of the drosophila ananassae species complex on penang island, malaysia. analyses of phenotypic traits, chromosome arrangements, molecular markers, and reproductive isolation suggest the existence of two species: d. ananassae and d. cf. parapallidosa. molecular marker analysis indicates that d. cf. parapallidosa carries chromosome y and 4 introgressions from d. ananassae. thus, d. cf. parapallidosa seems to be a hybrid descendant that recently originated from a natu ... | 2016 | 27715417 |
| fluctuating asymmetry in hybrids of sibling species, drosophila ananassae and drosophila pallidosa, is trait and sex specific. | due to inconsistent results of the empirical studies, the relationship between fluctuating asymmetry (fa, a measure of developmental stability) and interspecific hybridization has been the subject of intense debates. in the present study, we have assessed the impact of interspecific hybridization between 2 sibling species of drosophila: drosophila ananassae and drosophila pallidosa on the levels of fa over 3 generations. trait size of different morphological traits, namely, sternopleural bristle ... | 2009 | 18974399 |
| potential gene flow in natural populations of the drosophila ananassae species cluster inferred from a nuclear mitochondrial pseudogene. | a pseudogene with 94% similarity to mitochondrial cytochrome c oxidase subunit i (coi) was identified and localized to chromosome 4 of drosophila ananassae. because this chromosome is believed to have reduced recombination, its history can be traced using the pseudo-coi sequence. pseudo-coi sequences were obtained from 27 iso-female lines of six taxa belonging to the d. ananassae species cluster in which reproductive isolation is incomplete. the phylogenetic network constructed from seven recogn ... | 2008 | 18621132 |
| a locus for female discrimination behavior causing sexual isolation in drosophila. | the genetic basis of sexual isolation that contributes to speciation is one of the unsolved questions in evolutionary biology. drosophila ananassae and drosophila pallidosa are closely related, and postmating isolation has not developed between them. however, females of both species discriminate their mating partners, and this discrimination contributes to strong sexual isolation between them. by using surgical treatments, we demonstrate that male courtship songs play a dominant role in female m ... | 2001 | 11390998 |
| male remating in drosophila ananassae: evidence for interstrain variation in remating time and shorter duration of copulation during second mating. | in drosophila ananassae, male remating was studied using ten mass culture stocks which were initiated from flies collected from different geographic localities. male remating occurs at a high frequency and varies within narrow limits (84-96 percent) in different strains. interestingly, male remating time (in min) varies from 7.41 (bhutan) to 21.59 (pat) in different strains and the variation is highly significant. further, the results also show that males copulate for shorter duration during sec ... | 2000 | 18494595 |
| effects of photophase and altitude on oviposition rhythm of the himalayan strains of drosophila ananassae. | the effects of varying photophase and altitude of origin on the phase angle difference (psi) of the circadian rhythm of oviposition during entrainment to light-dark (ld) cycles and the aftereffects of such photophases on the period of the free-running rhythm (tau) in constant darkness (dd) were evaluated in two himalayan strains of drosophila ananassae, the high-altitude (ha) strain from badrinath (5,123 m above sea level=asl) and the low-altitude (la) strain from firozpur (179 m asl). the psi ( ... | 2007 | 17612939 |
| a site specific increase in recombination in drosophila ananassae. | the e65 pi; bri ru stock of drosophila ananassae produced an extremely high rate of recombination in males when made heterozygous with any one of the wild type stocks. we analyzed and characterized the genetic factors which caused this phenomenon. we show that the second chromosome of the e65 pi; bri ru stock carries an enhancer of male recombination. the enhancer, en(2)-ep, is located between om(2c) and arc. the enhancement of meiotic recombination both in males and females was also observed at ... | 2000 | 10846620 |
| twin intromittent organs of drosophila for traumatic insemination. | in several animals, male genitalia create insemination wounds in areas outside the genital orifice of females. i report that such traumatic insemination (ti) occurs in the drosophila bipectinata complex (diptera: drosophilidae) and illustrate a previously unknown evolutionary pathway for this behaviour. flash fixation of mating pairs revealed the dual function of the paired claw-like basal processes, previously misidentified as a bifid aedeagus: (i) penetration of the female body wall near the g ... | 2007 | 17519186 |
| latitudinal variation in eclosion rhythm among strains of drosophila ananassae. | eclosion rhythm parameters of d. ananassae strains originating between 8 degrees-34 degrees n were highly variable and latitude dependent. in the field under naturally fluctuating light intensity, temperature and r.h., the amplitude of the rhythm was high and the eclosion gate was narrow; however, under the naturally fluctuating light intensity but at constant temperature and r.h., the amplitude of the rhythm was lowered and the width of eclosion gate was widened. the eclosion rhythm entrained t ... | 1999 | 10522160 |
| chiasmata and chromosome breakages are related to crossing over in drosophila ananassae males. | a cytogenetic analysis of male crossing over in drosophila ananassae revealed that cytological exchanges resulted in genetic crossing over, and that chiasma frequency and the genetic recombination correlated positively in chromosomes 2 and 3. furthermore, the frequency of chromosome breakages correlated positively with chiasma frequency. paracentric inversion heterozygosity had no detectable influence on the chromosome pairing or exchange events within the inversion loop at meiosis. scoring of t ... | 2006 | 17426752 |
| effect of light intensity on the oviposition rhythm of the altitudinal strains of drosophila ananassae. | the sensitivity of the circadian photoreceptors mediating entrainment of the eclosion rhythm and phase shifts of oviposition rhythm of the high altitude (ha) strain of drosophila ananassae originating from badrinath (5123 m above sea level) in the himalayas was compared with the low altitude (la) strain from firozpur (179 m above sea level). reduced photic sensitivity of the ha strain is regarded as the result of natural selection, which led to the weakening of the coupling mechanism between the ... | 2007 | 17364577 |
| effects of altitude on circadian rhythm of adult locomotor activity in himalayan strains of drosophila helvetica. | we recently reported that the altitude of origin altered the photic and thermal sensitivity of the circadian pacemaker controlling eclosion and oviposition rhythms of high altitude himalayan strains of drosophila ananassae. the present study was aimed at investigating the effects of altitude of origin on the pacemaker controlling the adult locomotor activity rhythm of d. helvetica. | 2007 | 17210086 |
| comparative genomic analysis of allatostatin-encoding (ast) genes in drosophila species and prediction of regulatory elements by phylogenetic footprinting. | the role of the yxfgla family of allatostatin (ast) peptides in dipterans is not well-established. the recent completion of sequencing of genomes for multiple drosophila species provides an opportunity to study the evolutionary variation of the allatostatins and to examine regulatory elements that control gene expression. we performed comparative analyses of ast genes from seven drosophila species (drosophila melanogaster, drosophila simulans, drosophila ananassae, drosophila yakuba, drosophila ... | 2007 | 17175069 |
| fluctuating asymmetry in certain morphological traits in laboratory populations of drosophila ananassae. | fluctuating asymmetry (fa, subtle random deviations from perfect bilateral symmetry) is often used as a measure of developmental instability (di), which results from perturbations in developmental pathways caused by genetic or environmental stressors. during the present study, we estimated fa in 5 morphological traits, viz. wing length (wl), wing to thorax ratio (w:t), sternopleural bristle number (sbn), sex-comb tooth number (sctn), and ovariole number (on) in 18 laboratory populations of droso ... | 2006 | 16936786 |
| proceedings of the smbe tri-national young investigators' workshop 2005. unusual evolution of interspersed repeat sequences in the drosophila ananassae subgroup. | new repeat sequences were found in the drosophila ananassae genome sequence. they accounted for approximately 1.2% of the d. ananassae genome and were estimated to be more abundant in genomes of its closely related species belonging to the drosophila bipectinata complex, whereas it was entirely absent in the drosophila melanogaster genome. they were interspersed throughout euchromatic regions of the genome, usually as short tandem arrays of unit sequences, which were mostly 175-200 bp long with ... | 2006 | 16467489 |
| effect of directional selection for body size on fluctuating asymmetry in certain morphological traits in drosophila ananassae. | variation in the subtle differences between the right and left sides of bilateral characters or fluctuating asymmetry (fa) has been considered as an indicator of an organism's ability to cope with genetic and environmental stresses during development. however, due to inconsistency in the results of empirical studies, the relationship between fa and stress has been the subject of intense debate. in this study, we investigated whether stress caused by artificial bidirectional selection for body si ... | 2009 | 19550043 |
| the effects of larval density on adult life-history traits in three species of drosophila. | there is evidence that longevity and starvation resistance are determined by a common genetic mechanism. starvation resistance in drosophila strongly correlates with both fat content and longevity, and is affected by density during rearing. in this study, we examine how three species, drosophila melanogaster, drosophila ananassae and drosophila willistoni, respond to three larval density treatments. starvation resistance after adult eclosion, and after 2 days of feeding, and longevity were exami ... | 2005 | 15664627 |
| the role of natural selection in genetic differentiation of worldwide populations of drosophila ananassae. | the main evolutionary forces leading to genetic differentiation between populations are generally considered to be natural selection, random genetic drift, and limited migration. however, little empirical evidence exists to help explain the extent, mechanism, and relative role of these forces. in this study, we make use of the differential migration behavior of genes located in regions of low and high recombination to infer the role and demographic distribution of natural selection in drosophila ... | 2004 | 15611169 |
| effects of temperature, photoperiod, and light intensity on the eclosion rhythm of the high-altitude himalayan strain of drosophila ananassae. | eclosion rhythm of the high-altitude himalayan strain of drosophila ananassae from badrinath (altitude 5123 m) was temperature-dependent and at 21 degrees c, it was entrained by cycles of 12h light: 12h darkness (ld 12:12) and free-ran in constant darkness, however, it was arrhythmic at 13 degrees c or 17 degrees c under identical experimental conditions (khare, p. v., barnabas, r. j., kanojiya, m., kulkarni, a. d., joshi, d. s. (2002). temperature dependent eclosion rhythmicity in the high alti ... | 2004 | 15332442 |
| size dependent sexual selection in drosophila ananassae. | mate choice based on body size is widespread and can have numerous consequences. we present data, which show the effect of male and female body size on sexual selection in drosophila ananassae. the relationships between wing size, locomotor activity, mating latency, courtship pattern, fertility and mating success were studied. mating latency was negatively correlated with wing length and with locomotor activity, while wing length and locomotor activity was positively correlated in males as well ... | 2004 | 15330120 |
| genetic analyses of several drosophila ananassae-complex species show a low-frequency major gene for parthenogenesis that maps to chromosome 2. | parthenogenetic strains of several species have been found in the genus drosophila. the mode of diploidization in the eggs of females has been found to be post-meiotic nuclear fusion. the genetic basis for this parthenogenesis is not understood but is believed to be under the control of a complex polygenic system. we found parthenogenetic females in an isofemale strain (lae345) of d. pallidosa-like collected in 1981 at lae, papua new guinea, and established a parthenogenetically reproducing stra ... | 2004 | 15219153 |
| behavioral genetics of drosophila ananassae. | drosophila ananassae has a unique status among drosophila species because of certain peculiarities in its genetic behavior. the most unusual feature of this species is its relatively high frequency of spontaneous male recombination. the results of studies on non-sexual behavior, such as phototactic responses, eclosion rhythm, and preferences for oviposition and pupation sites, lead us to suggest that this behavior is under polygenic control, with a substantial amount of additive genetic variatio ... | 2003 | 15011143 |
| no evidence for minority male mating advantage in wild type strains of drosophila ananassae tested in multiple-choice experiments. | minority male mating advantage was tested in wild type strains of drosophila ananassae through multiple-choice experiments. mating success of two types of flies present in five different ratios was scored by direct observation in an elens-wattiaux mating chamber. we found no evidence for minority male mating advantage in wild type strains of d. ananassae. the relative mating success of two types of females was also compared in the multiple-choice experiments at different ratios; there was no evi ... | 2002 | 14963822 |
| morphogenetic and growth related changes in lipids of drosophila ananassae (doleschall). | the lipid component of drosophila ananassae have been identified qualitatively. neutral and phospholipid fractions are specific for pupal, larval or adult extracts. some of these lipid components are identical with those obtained from the temperate d. melanogaster and other species. this demonstrates biochemical convergence between geographically isolated species groups. | 2005 | 7511439 |
| selection for high and low pupation height in drosophila ananassae. | selection for high and low pupation height in drosophila ananassae was carried out for 10 generations. response to selection resulted in rapid divergence in pupation height in replicates of both high and low lines. there was a significant difference in mean pupation height among high, low, and control lines. regression coefficients for both high and low lines were significantly different from zero. the realized heritability over 10 generations was 45-49 for the high lines and 26-29 for the low l ... | 1993 | 8352717 |
| evidence for additive polygenic control of pupation height in drosophila ananassae. | in order to study the mode of inheritance of pupation height in drosophila ananassae, two mass culture stocks derived from ecogeographically different localities in india, were used to make a complete set of 16 crosses, which include parentals, f1, backcrosses, and f2. pupation height defined as the distance a larva pupates over the surface of culture medium was scored in all 16 crosses. the two parental lines showed significant difference in pupation height. the f1 larvae in both reciprocal cro ... | 1993 | 8106259 |
| population subdivision and molecular sequence variation: theory and analysis of drosophila ananassae data. | population subdivision complicates analysis of molecular variation. even if neutrality is assumed, three evolutionary forces need to be considered: migration, mutation, and drift. simplification can be achieved by assuming that the process of migration among and drift within subpopulations is occurring fast compared to mutation and drift in the entire population. this allows a two-step approach in the analysis: (i) analysis of population subdivision and (ii) analysis of molecular variation in th ... | 2003 | 14668389 |
| genetics of sexual isolation based on courtship song between two sympatric species: drosophila ananassae and d. pallidosa. | sexual isolation has been considered one of the primary causes of speciation and its genetic study has the potential to reveal the genetics of speciation. in drosophila, the importance of courtship songs in sexual isolation between closely related species has been well investigated, but studies analysing the genetic basis of the difference in the courtship songs associated with sexual isolation are less well documented. drosophila ananassae and drosophila pallidosa are useful for studies of sexu ... | 2002 | 12555781 |
| multiple amylase genes in drosophila ananassae and related species. | the number and organization of amylase genes in drosophila ananassae were investigated through classical genetic methods and in situ and filter hybridizations. at least four genes may be active in d. ananassae, organized as two independent pairs of closely linked copies on the 2l and 3l chromosomal arms. several other species of the d. ananassae subgroup were studied and show the same chromosomal locations, suggesting an ancient duplication event. however, the number of amy copies seems to be hi ... | 1992 | 1378417 |
| effect of directional selection on spontaneous male recombination in drosophila ananassae. | artificial selection was carried out for high and low spontaneous male recombination values in d. ananassae for nine generations by using cu b se marker (second chromosome) and wild stocks which were free from heterozygous chromosome inversions. the mean crossing-over frequency of nine generations was 2.22, 0.70 and 1.20% in high, low and control lines respectively. the values of regression coefficient and realized heritability also indicated that male recombination was affected by selection. ho ... | 1992 | 1506011 |
| search for species-specific mating signal in courtship songs of sympatric sibling species, drosophila ananassae and d. pallidosa. | sexual isolation is one of the most important mechanisms that may lead to speciation. drosophila ananassae and d. pallidosa are useful for the study of sexual isolation, because of their sympatric distribution and no postmating isolation between them. courtship songs are considered to play a crucial role in sexual isolation between d. ananassae and d. pallidosa. we recorded and analyzed male courtship songs of d. ananassae and d. pallidosa for eight and four geographical strains, respectively. c ... | 2002 | 12087192 |
| intra- and interspecies variations in pupation height in drosophila. | in order to study the pupation site preference which is an important component of larval behaviour, pupation height was scored in drosophila ananassae, d. bipectinata and d. malerkotliana by using a large number of strains of these species. the mean pupation height of different strains ranged from 1.1 to 8.7 mm in d. ananassae, from 0.41 to 0.75 mm in d. bipectinata and from 1.0 to 1.3 mm in d. malerkotliana. the analysis of variance and t-test were performed to test intre- and interspecies vari ... | 1991 | 1814833 |
| genetic analysis of the om(2d) locus in drosophila ananassae. | the om(2d)63 mutants were mutagenized by gamma-ray irradiation and deb feeding. a total of nine revertants were recovered and characterized; eight revertants were homozygous-lethal expressing no appreciable abnormality in cuticular pattern and central nervous system, and all failed to complement the lethality with each other. two of the eight expressed embryonic lethality and were associated with cytologically detectable deletions including the putative om(2d) locus, while four were associated w ... | 1991 | 1659433 |
| effect of temperature on longevity and productivity in drosophila ananassae: evidence for adaptive plasticity and trade-off between longevity and productivity. | the genetic response of body size to temperature in the laboratory provides an interesting example of phenotypic plasticity. we found that females of drosophila ananassae reared to adulthood at 18 degrees c showed significant increase in body weight as compared to females reared at 25 degrees c. at a given temperature, early productivity and lifetime productivity were the highest when the rearing and test temperature were the same. the effect of test temperature was highly significant for total ... | 2002 | 11990765 |
| identification of a different-type homeobox gene, barh1, possibly causing bar (b) and om(1d) mutations in drosophila. | the bar mutation b of drosophila melanogaster and optic morphology mutation om(1d) of drosophila ananassae result in suppression of ommatidium differentiation at the anterior portion of the eye. examinations was made to determine the genes responsible for these mutations. both loci were found to share in common a different type of homeobox gene, which we call "barh1." polyptides encoded by d. melanogaster and d. ananassae barh1 genes consist of 543 and 604 amino acids, respectively, with homeodo ... | 1991 | 1674606 |
| female remating in drosophila ananassae: bidirectional selection for remating speed. | in drosophila ananassae, artificial selection was carried out for fast and slow remating speed for 10 generations. response to selection resulted in rapid divergence in remating time in each of two replicates of both fast and slow lines. there were significant differences in mean remating time in females among fast, slow, and control lines. regression coefficients for both fast and slow lines are significantly different from zero. the realized heritability over 10 generations of selection is fro ... | 2001 | 11720122 |
| chill-coma temperature in drosophila: effects of developmental temperature, latitude, and phylogeny. | we modify and apply a nonlethal technique for rapidly quantifying the cold tolerance of large numbers of drosophila and other small insects. flies are transferred to individual vials, cooled in groups in progressive 0.5 degrees c steps, and checked for loss of righting response (chill-coma temperature [t(cc)]). flies recover quickly when transferred to ambient temperature, and thus this technique potentially can be used in selection experiments. we applied this technique in several experiments. ... | 2015 | 11331516 |
| molecular characterization and evolution of the amylase multigene family of drosophila ananassae. | drosophila ananassae is known to produce numerous alpha-amylase variants. we have cloned seven different amy genes in an african strain homozygous for the amy1,2,3,4 electrophoretic pattern. these genes are organized as two main clusters: the first one contains three intronless copies on the 2l chromosome arm, two of which are tandemly arranged. the other cluster, on the 3l arm, contains two intron-bearing copies. the amylase variants amy1 and amy2 have been assigned to the intronless cluster, a ... | 2000 | 11040291 |