| nucleoside triphosphate phosphohydrolase associated with cytoplasmic polyhedrosis virus. | nucleoside triphosphate phosphohydrolase ec 3.6.1.15 activity was found to be included in silkworm cytoplasmic polyhedrosis (cp) virus, which synthesizes mrna carrying the 5'-terminal modification. this enzyme releases orthophosphate from the gamma-position in a nucleoside triphosphate, leaving nucleoside diphosphate. the rate of hydrolysis of atp is faster than that of any other ribonucleoside triphosphate. deoxy atp is hydrolyzed rather faster than atp. however, polynucleotides carrying tripho ... | 1977 | 14944 |
| [comparative biochemical studies of polyhedral proteins of nuclear polyhedrosis viruses]. | using disc polyacrylamide gel electrophoresis, the molecular weights of polyhedral proteins of nuclear polyhedrosis viruses (npv) of porthetria dispar, mamestra brassicae, and aporia crataegi were found to be 28000 +/- 3000. it was shown that npv polyhedra of bombyx mori, galleria mellonella, p. dispar, and m. brassicae contain a protease. during dissolution of the polyhedra at ph 10,5 this protease specifically cleaves the matrix protein into 2--5 fragments. the amino acid compositions of npv p ... | 1978 | 33725 |
| the nucleotide sequence of human trnagly (anticodon gcc). | the sequence of trnagccgly from human placenta was determined by recently developed postlabeling techniques. the trna was digested completely with rnases t1 and a in the presence of alkaline phosphatase, the oligonucleotides were 3'-terminally (3h)-labeled, mapped on pei-cellulose thin layers, isolated, and sequenced by methods based on base-specific cleavages. overlaps were obtained by readout sequencing techniques on polyacrylamide gels and pei-cellulose thin layers. the thin-layer readout tec ... | 1979 | 116197 |
| phosphorylated sites of calf thymus histone h2b by adenosine 3',5'-monophosphate-dependent protein kinase from silkworm. | | 1975 | 170923 |
| studies on the sites in histones phosphorylated by adenosine 3':5'-monophosphate-dependent and guanosine 3':5'-monophosphate-dependent protein kinases. | adenosine 3':5'-monophosphate-dependent protein kinase (protein kinase a) purified from silkworm pupae phosphorylated five major fractions of calf thymus histone, whereas guanosine 3':5'-monophosphate-dependent protein kinase (protein kinase g) purified from the same organism reacted preferentially with h1, h2a, and h2b histones. amino acid analysis of the phosphopeptides which were obtained by proteolytic digestion revealed that both protein kinases a and g showed the abilities to phosphorylate ... | 1976 | 185207 |
| intrinsic activity of guanosine 3',5'-monophosphate-dependent protein kinase similar to adenosine 3',5'-monophosphate-dependent protein kinase. ii. phosphorylation of ribosomal proteins. | guanosine 3',5'-monophosphate (cyclic gmp)-dependent protein kinase purified from silkworm pupae reacts with rat liver ribosomal proteins when a stimulatory modulator (kuo, w.n. & kuo, j.f. 1976) j. biol. chem. 251, 4283-4286) is added to the reaction mixture. judging from autoradiogram of the radioactive proteins separated by electrophoresis on sodium dodecyl sulfate-polyacrylamide slab gel, the protein kinase utilizes the same proteins as those phosphorylated by adenosine 3',5'-monophosphate ( ... | 1977 | 197070 |
| the specific, diverse effects of purine and pyrimidine nucleoside-5'-di(tri)-3'-diphosphates on the eucaryote translation system in vitro. | all the eight 5'-di(tri)-3'-diphosphates of four common ribonucleosides were prepared by the enzymic pyrophosphoryl transfer catalysed by streptomyces adephospholyticus atp:nucleotide pyrophosphokinase (e.c.2.7.6.4) form datp to the respective 5'-phosphates, and their effects on the translation of mrnas by a wheat germ system in vitro were studied. (p) pppupp decreased the total 14c-leucine incorporation directed by a rat liver mrna whereas (p) pppypp did not. with a silkworm pupa ovary mrna, di ... | 1979 | 232758 |
| functional specificity of guanosine 3':5'-monophosphate-dependent and adenosine 3':5'-monophosphate-dependent protein kinases from silkworm. | adenosine 3':5'-monophosphate-dependent protein kinase partially purified from silkworm pupae shows identical functional activities with those of mammalian protein kinases; the insect and mammalian kinases are completely exchangeable in the phosphorylation of muscle glycogen phosphorylase kinase and glycogen synthetase resulting in the activation and inactivation of the respective enzymes. in contrast, guanosine 3':5'-monophosphate-dependent protein kinase obtained from the same organism is tota ... | 1975 | 234451 |
| the properties of two recent isolates of cytoplasmic polyhedrosis viruses. | cytoplasmic polyhedrosis viruses (cpvs) were isolated from laboratory cultures of bombyx mori and spodoptera exempta. the electrophoretic profile of the rna segments of both viruses showed similarities with that of type 1 cpv. however, whereas the virus isolate from b. mori appeared identical to type 1 cpv (as assessed by serology, as well as the comparative mobilities of rna segments and structural polypeptides), the isolate from s. exempta was distinct. three of the ten viral rna segments did ... | 1977 | 323189 |
| bactericidal activity of the normal, cell-free hemolymph of silkworms (bombyx mori). | cell-free hemolymph from silkworm (bombyx mori) larvae can kill escherichia coli b/sm. the bactericidal principle can be resolved into at least two factors. one is a lysozyme-like enzyme that can be absorbed on crab shell chitin and on bentonite, and the other (cofactor) is an anionic factor that is of low molecular weight, can pass through the chitin column and a carboxymethyl-cellulose column, and can be eluted from a diethylaminoethyl-cellulose column at mu = 0.15 and ph 7.5. egg-white lysozy ... | 1977 | 326674 |
| cloning of the silk fibroin gene and its flanking sequences. | thirteen escherichia coli clones containing the whole or a part of the fibroin gene (16 kilobases long) have been isolated. the starting material was dna extracted from the posterior silk glands of bombyx mori. most clones were obtained from sheared dna fragments linked by poly(da)-poly(dt) joints to the plasmid pmb9. one of them includes the 5' end of the fibroin gene with a flanking sequence of 12 kilobases, and another includes the 3' end of the gene with a flanking sequence of about 1 kiloba ... | 1977 | 341154 |
| immunoperoxidase detection of baculovirus antigens in insect cells. | the sequence of events in the infection of tn-368-10 and tn-368-13 cells by autographa californica nuclear polyhedrosis virus (acmnpv) was investigated by using the indirect immunoperoxidase technique. antisera raised against enveloped nucleocapsids detected homologous antigens at 6 to 8 h post infection which was about 2 h before the appearance of both intracellular and extracellular infectious virus. similar tests using polyhedrin antiserum showed that polyhedrin is first synthesized at 12 h p ... | 1978 | 357682 |
| the genes for 18s, 5.8s and 28s ribosomal rna of bombyx mori are organized into tandem repeats of uniform length. | the organization of the multiple genes for 18s, 5.8s and 28s rrna in the genome of the silkworm, bombyx mori was determined by restriction endonuclease digestion and southern blot hybridization. the ribosomal genes (rdna) are tandemly reiterated, with a uniform repeat length of 6.9 . 10(6) daltons. each rdna repeat has a single site for ecori, hindiii, hpai and smai and each of these sites has been mapped with respect to the others and to the rrna genes; each repeat consists of a transcribed reg ... | 1978 | 363521 |
| in vitro processing of b. mori transfer rna precursor molecules. | ribonuclease p and 3'-5' nuclease, two enzymatic activities necessary for trna synthesis in e. coli, are also found in the silkgland cells of bombyx mori. b. mori subcellular extracts containing rnaase p activity can cleave the e. coli trna precursor molecule endonucleolytically at the same site as the e. coli enzyme, and will also cleave in vitro all e. coli trna precursors (pre-trnas) which the bacterial enzyme recognizes. b. mori rnaase p will not cleave two e. coli rnaase p substrates that a ... | 1979 | 378409 |
| homology of the 3' terminal sequences of the 18s rrna of bombyx mori and the 16s rrna of escherchia coli. | the terminal 220 base pairs (bp) of the gene for 18s rrna and 18 bp of the adjoining spacer rdna of the silkworm bombyx mori have been sequenced. comparison with the sequence of the 16s rrna gene of escherichia coli has shown that a region including 45 bp of the b. mori sequence at the 3' end is remarkably homologous with the 3' terminal e. coli sequence. other homologies occur in the terminal regions of the 18s and 16s rrnas, including a perfectly conserved stretch of 13 bp within a longer homo ... | 1979 | 390496 |
| [ultracytological studies of the morphogenesis and structure of the nuclear polyhedrosis virus in the silkworm moth, bombyx mori l]. | an electron-microscopic investigation was made on hypodermal tissues of silkworms spontaneously infested by nuclear polyhedral disease, due to low temperature stress. it was established that the polyhedral virus is formed in the viroplasma produced after viral cell infection, is included in the intimate and developing membrane singly or in fascicles containing several (up to 9) and is finally incapsulated in polyhedral protein. besides viral rods, various polymorphic forms were observed in the n ... | 1979 | 392906 |
| direct atomization atomic absorption spectrometry for intact biological samples: application to trace metal analysis in silkworm eggs and their hatched worms. | | 1979 | 464310 |
| effect of starvation on trna synthesis, amino acid pool, trna charging levels and aminoacyl-trna synthetase activities in the posterior silk gland of bombyx mori l. | changes in the translational machinery components of the bombyx mori posterior silk gland were analysed during starvation and refeeding and compared to the regularly fed larvae. during starvation, trna and ribosomal rna synthesis are stopped. the amounts of different rna classes and of the different trna species slow down at the same rate. thus various trna show similar half-lifes and the preexisting trna adaptation to fibroin mrna translation persists during starvation. similarly, the trna/rrna ... | 1979 | 465573 |
| insect lysozymes from three species of lepidoptera: their structural relatedness to the c (chicken) type lysozyme. | sequence studies of the n-terminal halves of the lysozymes isolated from bombyx mori, gallera mellonella and spodoptera littoralis (lepidoptera) allow us to classify these enzymes among the c (chicken) type lysozymes. | 1979 | 537106 |
| comparative ultrastructural studies of insect granulosis and nuclear polyhedrosis viruses. | optical diffraction studies indicated that the periodic lattice structure in electron micrographs of the capsids of two granulosis and two nuclear polyhedrosis viruses were indistinguishable. the capsid is composed of stacked rings spaced 4-5 nm apart. comparison of the intracellular forms of bombyx mori nuclear polyhedrosis virus with negatively stained virus particles leads to encourage the more general use of the term 'capsid' instead of 'intimate membrane' and the term 'virus membrane' in pl ... | 1976 | 778335 |
| [isolation and characteristics of fragments from the cyanogen bromide splitting of inclusion body proteins of bombyx mori nuclear polyhedrosis virus]. | the cyanogen bromide cleavage of "acetic" polyhedral protein from nuclear polyhedrosis virus of b. mori was performed. two peptides brcn-ii and brcn-ii' which consist of 29 and 34 amino acid residue were isolated from a mixture of cyanogen bromide fragments. the amino acid composition and n-terminal amino acids were established. the tryptic proteolysis of these fragments was conducted and the tryptic peptides were separated. the amino acid composition of tryptic peptides was determined. the data ... | 1976 | 797077 |
| [secondary structure of the polyhedral protein of the nuclear polyhedrosis virus of bombyx mori and some of its fragments]. | the secondary structure of polyhedral protein of the nuclear polyhedrosis virus of bombyx mori and some of its fragments has been investigated by circular dichroism and optical rotatory dispersion. it has been shown that the protein contains 6% alpha-helices and 26% beta-structures at ph 10.5. the conversion of beta-pleated sheets to alpha-helices after the treatment with sodium dodecyl sulfate was observed. a correlation between the number of alpha-helices in the fragment brcn-v and its ability ... | 1976 | 802784 |
| detection of aflatoxin b1 in silkworm larvae attacked by an aspergillus flavus isolate from a sericultural farm. | aflatoxin b(1) (0.05 mum per larvae) was detected in silkworm larvae artificially attacked by an aspergillus flavus isolate from a sericultural farm in japan. | 1975 | 813577 |
| the blocked and methylated 5' terminal sequence of a specific cellular messenger: the mrna for silk fibroin of bombyx mori. | the messenger rna for silf fibroin, laveled with 32po4 and methyl-3h l-methionine, was purified to near homogeneity from the posterior silk gland of the sildworm bombyx mori, and the sequence of a methylated, rnaase t2-resistant structure was determined. this sequence is similar structurally to 5' terminal blocked and methylated sequences found on the total populations of polyadenylated eucaryotic cellular and certain viral mrnas. the rnaase t2-resistant oligomer from fibroin mrna was cleaved by ... | 1976 | 947545 |
| further studies on the genetic effects of furylfuramide on mature spermatozoa of the silkworm [proceedings]. | | 1976 | 972630 |
| mapping of deoxydi- and trinucleotides liberated by the action of endonucleases from the silkworm and aspergillis oryzae. | both silkworm nuclease and nuclease o of aspergillus oryzae mycelia hydrolyse dna endolytically to di- and trinucleotides terminating in 5'-phosphate. these oligonucleotides were fractionated first be deae-cellulose chromatography with 7 m urea into the respective isoplithic groups and then analysed for the composition and isomerism: each group was labeled 5'-terminally by the [gamma-32p]atp-polynucleotide kinase reaction and then electrophoresed monodimensionally for the dinucleotides and two-d ... | 1978 | 656423 |
| structural studies on rna from bombyx mori l. i. nucleoside composition of enriched trna species from the posterior silkgland purified by coutercurrent distribution. | a large scale fractionation of trna from the posterior silkgland of the silkworm bombyx mori l. by countercurrent distribution is described. one single 1,500 transfer distribution carried out with phosphate buffer-fromamide-isopropanol (pfi) solvent system yields highly enriched isoaccepting species with increasing mobility order: trna1gly, trna1-2ala, trnatyr, trna2gly, trna1ser and trna2ser with 75%, 70%, 90%, 60%, 60%, and 90% purities respectively. nucleosides fingerprint analysis of each is ... | 1976 | 999951 |
| serial passage of a nuclear polyhedrosis virus of the silkworm, bombyx mori, in larvae of rice stem borer, chilo suppressalis. | | 1975 | 1089731 |
| antibodies ot double-stranded rna: specificity and serum nucleases. | an antiserum was prepared in rabbits to the synthetic double-stranded ribonucleic acid (ds rna) poly ri:rc. using a liquid-phase radioimmunoassay, the antiserum cross-reacted with a natural ds rna isolated from the cytoplasmic polyhedrosis virus of the silkworm, binding 95% of the rna at a 1 : 20 serum dilution. preliminary tests of the specificity of the antiserum showed that it did not bind single-stranded rna (ss rna) or deoxyribonucleic acid (dna), but also revealed that the serum contained ... | 1975 | 1107429 |
| formation of temporary flagellar structures during insect organogenesis. | cilia and flagella are rare in nongerminal tissues of anthropods, and are generally thought to be restricted to sperm and sensory cells in insects (2). whitten (5) has reported the presence of kinetosomes at the base of mitotrichia in the dipteran fly sarcophaga bullata, but reports no evidence of the organization of fibrous elements characteristic of cilia and or flagella. during an ultrastructural analysis of morphogenesis of the colleterial gland of the silk moth hyalophora cecropia, we found ... | 1975 | 1127020 |
| nucleotide sequence of bombyx mori l. trna1gly. | | 1977 | 333294 |
| [use of silkworm silk gland ribosomes as a model for the study of exogenous matrices in protein biosynthesis]. | | 1976 | 1269391 |
| chymotrypsin inhibitors from hemolymph of the silkworm, bombyx mori. | three new protease inhibitors were isolated and purified about 200-fold from hemolymph of silkworm larvae, bombyx mori, using ion-exchange and affinity chromatography. two of the three inhibitors were basic proteins (sci-i had pi 9.4 and sci-ii had pi 9.6) and one was acidic (sci-iii had pi 4.0). the molecular weight of each inhibitor was determined to be 7,000 by the sedimentation equilibrium method. the amino acid composition of the inhibitors were similar except for the contents of asp, glu, ... | 1978 | 29886 |
| evidence for two immune inhibitors from bacillus thuringiensis interfering with the humoral defense system of saturniid pupae. | mutants of bacillus thuringiensis lacking either beta-exotoxin or gamma-endotoxin were compared for their virulence using pupae of a giant silk moth. known doses of viable log-phase bacteria were injected, and the response was followed as the number of viable bacteria in the hemolymph. the results obtained imply that, in the system used, neither the beta-exotoxin nor the gamma-endotoxin and the sporeforming ability are of importance for virulence. results with sterile culture filtrate from b. th ... | 1976 | 992874 |
| location of a bombyx mori receptor binding region on a bacillus thuringiensis delta-endotoxin. | receptor binding studies were performed with 125i-labeled trypsin-activated insecticidal toxins, cryia(a) and cryia(c), from bacillus thuringiensis on brush-border membrane vesicles (bbmv) prepared from bombyx mori larval midgut. bioassays were performed by gently force feeding b. mori with diluted toxins. cryia(a) toxin (ld50; 0.002 micrograms) was 200 times more active against b. mori larvae than cryia(c) toxin (ld50; 0.421 micrograms) and showed high-affinity saturable binding. the kd and the ... | 1992 | 1310681 |
| translation of silk fibroin messenger rna in an ehrlich ascites cell-free extract. | rna identified by its base composition and t1 rnase oligonucleotide pattern as the message for silk fibroin was purified from mature posterior silk glands of bombyx mori larvae and used to direct polypeptide synthesis in an ehrlich ascites cell-free extract. fibroin mrna stimulated [3-h]alanine incorporation about 3- to 4-fold in the presence of 80 mm k+ and 4 mm mg-2+. the stimulation was reduced in the presence of 5 times 10-minus 6 to 10-minus 4 m aurintricarboxylic acid, an inhibitor of the ... | 1975 | 1171097 |
| processing of delta-endotoxin from bacillus thuringiensis subsp. kurstaki hd-1 and hd-73 by gut juices of various insect larvae. | midgut juices were prepared from adoxophyes sp., smaller tea tortrix (stt); bombyx mori, silkworm (sw); spodoptera litura, common cutworm (ccw); plutella xylostella, diamondback moth (dbm); and musca domestica, housefly (hf) and immobilized onto sepharose 4b. delta-endotoxins (icps) from bacillus thuringiensis subsp. kurstaki hd-1 and hd-73 were digested by these immobilized gut juice proteases. all gut juices tested derived relatively proteolytic resistant cores from icp. the molecular sizes of ... | 1992 | 1328398 |
| expression and characterization of kinase-active v-erbb protein using a baculovirus vector system. | the v-erbb gene is an oncogene of the avian erythroblastosis virus encoding a protein that is a truncated version of the epidermal growth factor receptor. the v-erbb protein was expressed alone or as polyhedrin-erbb fusion proteins using the bombyx mori nuclear polyhedrosis virus vector. the expression level of the fusion protein whose polyhedrin portion consisted of only 8 amino-terminal amino acids was more than ten times higher than that of the non-fusion protein. studies with tunicamycin sho ... | 1992 | 1347525 |
| purification and refolding of recombinant human igf ii from silkworms infected with recombinant bombyx mori nuclear polyhedrosis virus. | we have reported that insulin-like growth factor ii (igf ii) was produced as a fusion protein in bombyx mori (silkworm) larval bodies infected with recombinant b. mori nuclear polyhedrosis virus [j. gen. virol., 68, 2599-2606 (1987)]. in this study, the purification of igf ii from the infected silkworms is reported. the fusion protein was extracted with 6.0 m guanidine-hcl from the infected larval bodies homogenized in water. the use of organic solvents to remove the impurities, such as lipid de ... | 1992 | 1368125 |
| limited proteolysis of silkworm antitrypsin by several proteinases. | silkworm antitrypsin (sw-at) isolated from larval hemolymph was limitedly digested by achromobacter lysylendopeptidase, alpha-chymotrypsin, subtilisin bpn', subtilisin carlsberg, papain, or pseudomonas elastase. each proteinase could cleave specific site(s) around the reactive site identified for the reaction of sw-at and bovine trypsin. among these proteinases, only subtilisin bpn' was inhibited by sw-at, although weakly. by the cleavable amino acid sequence in sw-at, it was suggested that whet ... | 1990 | 1368515 |
| isolation and partial characterization of binding proteins for immobilized delta endotoxin from solubilized brush border membrane vesicles of the silkworm, bombyx mori, and the common cutworm, spodoptera litura. | 1. brush border membrane vesicles (bbmv) were prepared from the bombyx mori, and spodoptera litura, midguts. bbmv was solubilized. 2. activated delta endotoxin from bacillus thuringiensis were immobilized. 3. solubilized bbmv proteins were applied to the toxin column and the proteins bound were analyzed by sds-page. 4. in the case of b. mori m(r) = 220,000, 150,000 and 130,000 and in the case of s. litura 160,000 bands were detected. 5. the bindings were inhibited by n-acetyl galactosamine and m ... | 1992 | 1323444 |
| the properties of monoclonal antibody against sepiapterin reductase from fat body of the silkworm, bombyx mori. | a specific monoclonal antibody prepared for the 29-kda a subunit of silkworm fat body sepiapterin reductase (spr) was able to recognize the subunit in crude extract of fat body after sds treatment. although spr from the silkworm fat body has biochemical properties similar to those reported for spr from mammalian sources, especially rat erythrocytes, the antibody failed to recognize the 28-kda subunit of rat erythrocyte spr. this result indicates that spr from silkworm fat body has a different am ... | 1992 | 1292509 |
| brain secretory peptides of the silkmoth bombyx mori: prothoracicotropic hormone and bombyxin. | | 1992 | 1302870 |
| feline interferon production in silkworm by recombinant baculovirus. | | 1992 | 1379476 |
| structure and expression of gene coding for a pupal cuticle protein of bombyx mori. | a specific protein termed as pcp accumulates in the newly synthesized pupal cuticle of the silkworm, bombyx mori. we have cloned the genomic sequence encoding pcp and analyzed its structure. the pcp gene comprises two exons interspersed by a single intron approx. 5.8 kb in length. transcription initiation sites of the pcp gene were located at nucleotide level. the 5' flanking region of the gene contains a sequence homologous to the pit-1 dna recognition element of the rat prolactin and growth ho ... | 1992 | 1390888 |
| high level expression of a frog alpha-amidating enzyme, ae-ii, in cultured cells and silkworm larvae using a bombyx mori nuclear polyhedrosis virus expression vector. | a xenopus laevis peptidyl c-terminal alpha-amidating enzyme (ae-ii) gene, modified by deletion of a region encoding the putative membrane-spanning domain and the putative c-terminal cytosolic tail, was expressed in bomo-15 aiic insect cells and silkworm larvae using a bombyx mori baculovirus expression vector system. the expressed enzyme was identified predominantly in the culture medium and the hemolymph of silkworm larvae, indicating successful secretion of the expressed ae-ii. the level of re ... | 1992 | 1368810 |
| expression of a putative precursor of pheromone biosynthesis activating neuropeptide-i (pban-i) of the silkmoth, bombyx mori, and its conversion to the mature peptide, pban-i. | | 1991 | 1368723 |
| complete nucleotide sequence of the cloned infectious genome of junonia coenia densovirus reveals an organization unique among parvoviruses. | we previously constructed a recombinant plasmid, pbrj, encompassing an infectious junonia coenia densovirus (jcdnv) genome (m. jourdan et al. (1990). virology 179, 403-409). we report here the complete viral sequence of pbrj. the genome, 5908 nucleotides (nt) long, consists of an internal unique sequence flanked by long (517 nt) inverted terminal repeats. the first 96 bases of one extremity can fold into a typical y-shaped hairpin structure. the opposite extremity is incomplete, lacking 88 nt. t ... | 1992 | 1413502 |
| biochemical and immunological characterization of the dna binding protein (rbp-j kappa) to mouse j kappa recombination signal sequence. | we have investigated whether j kappa recombination signal sequence (rs) binding protein (rbp-j kappa) has any partial catalytic activities involved in the vdj recombination reaction, such as cleavage, ligation, and bending of dna. murine rbp-j kappa protein purified by j kappa-rs affinity chromatography did not show dna cleavage activities but contained a strong dna ligase activity. to obtain a large amount of purified rbp-j kappa protein, recombinant rbp-j kappa was synthesized in escherichia c ... | 1992 | 1429518 |
| antibody production in silkworm cells and silkworm larvae infected with a dual recombinant bombyx mori nuclear polyhedrosis virus. | we have examined the efficiency of coexpression of two heterologous genes from a recombinant bombyx mori nuclear polyhedrosis virus for the production of antibodies in silkworm larvae. the cdnas encoding the light and the heavy chains of a murine immunoglobulin, directed against lipoprotein i of pseudomonas aeruginosa, were brought under the control of two separate copies of the viral polyhedrin promotor. infection of silkworm cells with the recombinant baculovirus yielded a maximum of 6.4 micro ... | 1992 | 1368987 |
| processing of structural polypeptides of infectious flacherie virus of the silkworm, bombyx mori: vp1 and vp4 are derived from vp0. | infectious flacherie virus of the silkworm, bombyx mori, contains five major polypeptides termed vp0, vp1, vp2, vp3, and vp4 in the order of descending molecular weight. immunoblot analysis with specific antisera against each of these structural polypeptides showed that antisera against vp1 and vp4 unequivocally reacted with vp0 as well as their homologous structural polypeptides. limited proteolysis of vp0 and vp1 by staphylococcus aureus v8 protease gave several common polypeptide fragments. a ... | 1992 | 1401988 |
| determination of the glycosylation patterns, disulfide linkages, and protein heterogeneities of baculovirus-expressed mouse interleukin-3 by mass spectrometry. | the primary structure of mouse interleukin-3 (il-3) expressed by recombinant baculovirus-infected silkworm (bombyx mori) larvae was analyzed by subjecting isolated il-3 derived peptides to liquid secondary ion mass spectrometry. two species of il-3 were isolated from the silkworm hemolymph by reverse-phase high-pressure liquid chromatography. the major component has m(r)20-22 x 10(3) as determined by sds-page. liquid secondary ion mass spectrometric analysis was carried out on the reduced trypti ... | 1992 | 1445902 |
| cloning and sequencing of the cdna encoding rice elongation factor 1 beta'. | a cdna clone coding for rice elongation factor 1 beta' (ef-1 beta') was isolated from a rice anther cdna library. the clone, named rb', was 980 bp long and contained a single open reading frame coding for 223 amino acids; the first 31 amino acids, except for the first methionine, which is absent in the mature protein, are identical to those of the purified protein determined with a protein sequencer. the amino acid sequence of rice ef-1 beta' shows homology to the c-terminal half of artemia sali ... | 1992 | 1397289 |
| characterization of silkworm chlorophyll metabolites as an active photosensitizer for photodynamic therapy. | silkworm excreta containing chlorophyll metabolites have long been used for their medicinal activities in the far east. the major chlorophyll derivative (cpd) fraction in the me2co extract from the silkworm excreta exhibits strong photodynamic action on mice and human tumor cells in vitro. the main cpd component was isolated, purified, and subjected to extensive structural and characterization studies to assess its photodynamic therapeutic activity. according to the spectral analyses, the struct ... | 1992 | 1431943 |
| development of an antibody against a 170-kda fragment of fibroin isolated from cocoon fibres of bombyx mori. | a simple method for the isolation of denatured fragments of the fibroin protein from cocoon fibres by alkali solubilization is discussed. this 170-kda antigen has been purified and used to raise the polyclonal antibody in rabbit. the specificity of this antibody to the purified cocoon protein has suggested strong immunoreactivity up to a titre of 1:5000 dilution of the antibody. further, dot-blot analysis with the tissue extracts from silk glands of different larval stages (3rd to 5th) reveals t ... | 1992 | 1430790 |
| transient expression assay in a baculovirus system using firefly luciferase gene as a reporter. | transient gene expression assays were developed to assess the function of the regulatory sequences of baculoviruses bombyx mori nuclear polyhedrosis virus (bmnpv) and autographa californica nuclear polyhedrosis virus (acnpv) in insect cells of bombyx mori and spodoptera frugiperda, respectively. dna sequences encoding luciferase (luc) of the firefly photinus pyralis was successfully employed in the expression assay as a reporter gene. recombinant plasmids were constructed containing the luc gene ... | 1992 | 1475909 |
| assignment of the magnetic resonances of the imino protons and methyl protons of bombyx mori trna(glygcc) and the effect of ion binding on its structure. | the magnetic resonances in the low-field h-nmr spectra of bombyx mori trna(glygcc), corresponding to the hydrogen-bonded imino protons of the helical stems and tertiary base pairs, could be tentatively assigned by means of the sequential nuclear overhauser effects. while b. mori trna(glygcc) does not contain the g19c56 tertiary base pair, the d20g57 base pair exists between the d and t loops, which was not found in the x-ray crystal structure of yeast trna(phe). the effects of mg2+, spermine and ... | 1992 | 1483452 |
| a novel dna-binding motif abuts the zinc finger domain of insect nuclear hormone receptor ftz-f1 and mouse embryonal long terminal repeat-binding protein. | fruit fly ftz-f1, silkworm bmftz-f1, and mouse embryonal long terminal repeat-binding protein are members of the nuclear hormone receptor superfamily, which recognizes the same sequence, 5'-pycaaggpycpu-3'. among these proteins, a 30-amino-acid basic region abutting the c-terminal end of the zinc finger motif, designated the ftz-f1 box, is conserved. gel mobility shift competition by various mutant peptides of the dna-binding region revealed that the ftz-f1 box as well as the zinc finger motif i ... | 1992 | 1448096 |
| antibacterial activity inducible in the haemolymph of the silkworm, bombyx mori, by injection of formalin-treated escherichia coli k-12 during the fifth larval instar and pharate adult development. | 1. antibacterial activity inducible in the haemolymph of the silkworm, bombyx mori, by immunization, i.e. by injection of formalin-treated escherichia coli (e. coli) k-12 during the fifth larval instar and pharate adult development that was reared aseptically on an artificial diet was determined by inhibition zone assay using the same bacterium as a test organism. 2. a peak of antibacterial activity was observed in each development stage; approximately 8 mm in diameter of a clear zone at days 3 ... | 1992 | 1499263 |
| induction of antibacterial activity in the haemolymph of the silkworm, bombyx mori, by injection of formalin-treated escherichia coli k-12 in the anterior and posterior body part of the ligated larvae. | 1. induction of antibacterial activity was investigated in the ligated fifth instar larvae of the silkworm, bombyx mori, by injection of formalin-treated escherichia coli k-12 into the haemocoel in the anterior and in the posterior body part, followed by activity determination by inhibition zone assay of the haemolymph at 12 and 24 hr after immunization. 2. at 12 hr after immunization, high antibacterial activity, approximately 6.8-7.5 mm in the anterior body part and 4.5-6.4 mm in the posterior ... | 1992 | 1499265 |
| oral immunization with escherichia coli k-12 of the fifth instar larvae of the silkworm, bombyx mori, reared on an artificial diet under completely aseptic conditions. | 1. effect of oral administration of live or formalin-treated escherichia coli (e. coli) k-12 to the fifth instar, days 1 and 3 larvae of the silkworm, bombyx mori, on induction of antibacterial activity in the haemolymph was investigated using the silkworms reared on an artificial diet under completely aseptic conditions. 2. when live e. coli was administered to the male day 1 larvae, low but significant antibacterial activity of 3.8 mm was detectable in the haemolymph of one individual at 48 hr ... | 1992 | 1499266 |
| distinct n-glycan fucosylation potentials of three lepidopteran cell lines. | the fucosyltransferase activities of three insect cell lines, mb-0503 (from mamestra brassicae), bm-n (from bombyx mori) and sf-9 (from spodoptera frugiperda), were investigated and compared with that of honeybee venom glands. cell extracts and venom gland extracts were incubated with gdp-[14c]fucose and glycopeptides isolated from human igg and from bovine fibrin. the labeled oligosaccharide products were released by peptide-n4-(n-acetyl-beta-glucosaminyl)asparagine amidase a, fluorescence mark ... | 1992 | 1499571 |
| [stimulation of replicative dna synthesis by eukaryotic proteins bound to single-stranded dna]. | the paper deals with the effect of the single-strand (ss) dna-binding proteins (ssb-proteins) from the ehrlich ascites tumor (eat) cells and from the eggs of silkworm, as well as the mouse serum blood proteins, having preferential affinity to ss dna, on the dna replicative synthesis in the eat cells permeable for the macromolecules, and, for the silkworm proteins and on the dna replicative synthesis in the nuclei from the eggs of silkworm proteins and on the dna replicative synthesis in the nucl ... | 1992 | 1519344 |
| a cellular promoter-based expression cassette for generating recombinant baculoviruses directing rapid expression of passenger genes in infected insects. | we have developed an expression cassette which allows the generation of recombinant baculoviruses that can express passenger genes under the control of a constitutive cellular promoter derived from the cytoplasmic actin gene of the silkmoth bombyx mori. silkmoth tissue culture cells which were infected with a recombinant b. mori nuclear polyhedrosis virus (bmnpv) containing the gene-encoding chloramphenicol acetyltransferase (cat) under the control of this expression cassette expressed significa ... | 1992 | 1519361 |
| biosynthesis of nad-sorbitol dehydrogenase is induced by acclimation at 5 degrees c in diapause eggs of the silkworm, bombyx mori. | 1. in diapausing eggs of the silkworm, bombyx mori, activity of nad-sorbitol dehydrogenase (ec 1.1.1.14, sdh) is almost negligible, but is increased by acclimation at 5 degrees c (yaginuma et al., 1990, j. comp. physiol. b160, 277-285). to elucidate the mechanism regulating sdh activity, the following experiments were conducted. anti-sdh serum was made in a mouse using purified sheep liver sdh. 2. this antiserum reacted with bombyx egg sdh purified partially by blue sepharose cl-6b and sephacryl ... | 1992 | 1526125 |
| nucleotide sequence of a transactivating bombyx mori nuclear polyhedrosis virus immediate early gene. | the open reading frame (orf) of 1572 bp contained in the 3.8 kb clai fragment of bmnpv encodes a viral regulatory protein which transactivates the delayed early acmnpv 39k gene. transactivation is induced in uninfected cells following transfection with a plasmid containing only the clai fragment. hitherto immediate early gene promoter activity of the included 631 bp leader sequence is evident since no other viral elements are needed for the transcription of the regulatory gene. | 1992 | 1536885 |
| induction of a novel protein kinase in pupae of the silkworm bombyx mori after infection with nuclear polyhedrosis virus. | protein kinases induced by bombyx mori nuclear polyhedrosis virus in pupae of the silkworm b. mori were examined by activity gel analysis using phosvitin as a protein substrate. the method involved page of the soluble fraction from pupae under native conditions and in the presence of sds, followed by in situ renaturation of proteins and recovery of protein kinase activity in the intact gel. a novel protein kinase able to phosphorylate phosvitin was detected in the infected pupae from 2 days post ... | 1992 | 1469362 |
| the anticarsia gemmatalis nuclear polyhedrosis virus polyhedrin gene region: sequence analysis, gene product and structural comparisons. | the genomic region of the anticarsia gemmatalis multiple nucleocapsid nuclear polyhedrosis virus (agmnpv) strain 2d encoding the polyhedrin gene was cloned and mapped, and a 2085 bp sphi-psti fragment containing the gene was sequenced. the polyhedrin polypeptide of the parental isolate agmnpv was manually sequenced, and the amino acid sequence obtained agreed with that deduced from the dna coding region sequence. agmnpv and orgyia pseudotsugata mnpv (opmnpv) are similar in terms of promoter stru ... | 1992 | 1588315 |
| insecticidal properties of a crystal protein gene product isolated from bacillus thuringiensis subsp. kenyae. | a protoxin gene, localized to a high-molecular-weight plasmid from bacillus thuringiensis subsp. kenyae, was cloned on a 19-kb bamhi dna fragment into escherichia coli. characterization of the gene revealed it to be a member of the cryie toxin subclass which has been reported to be as toxic as the cryic subclass to larvae from spodoptera exigua in assays with crude e. coli extracts. to directly test the purified recombinant gene product, the gene was subcloned as a 4.8-kb fragment into an expres ... | 1992 | 1610185 |
| protein synthesis in pupae of the silkworm bombyx mori after infection with nuclear polyhedrosis virus: resistance to viral infection acquired during pupal period. | protein synthesis has been studied in pupae of the silkworm bombyx mori (bm) infected with nuclear polyhedrosis virus (bmnpv) at various stages of the pupal period. nascent proteins were labelled by injection of [35s]methionine into pupae and then analysed by sds-page. temporal regulation of synthesis of infected cell-specific proteins (icsps) in pupae was demonstrated by electrophoretic analysis of the proteins labelled at different times post-infection (p.i.). the rate of icsp synthesis reache ... | 1992 | 1469357 |
| foreign gene expression by a baculovirus vector with an expanded host range. | a nuclear polyhedrosis virus (npv) (baculoviridae)-based gene expression system was improved by dna recombination. the bmn cell line established from bombyx mori and the sf21 cell line established from spodoptera frugiperda are non-permissive for autographa californica multicapsid npv (acmnpv) and b. mori npv (bmnpv) replication, respectively. after cotransfection of acmnpv dna and bamhi-digested bmnpv dna into sf21 cells, progeny viruses were isolated by plaque purification on bmn cell monolaye ... | 1992 | 1629707 |
| nucleotide sequence and transcriptional analysis of a gene encoding gp41, a structural glycoprotein of the baculovirus autographa californica nuclear polyhedrosis virus. | we have identified and sequenced a region of the autographa californica nuclear polyhedrosis virus (acmnpv) genome encoding the major polyhedron-derived virus structural glycoprotein gp41. the open reading frame is located entirely within the acmnpv sstii-m fragment. the protein sequence does not have hydrophobic regions characteristic of integral membrane proteins and contains a putative o-linked glcnac glycosylation site. gp41 is expressed as a late gene, with transcripts starting within two c ... | 1992 | 1629955 |
| dna superhelicity affects the formation of transcription preinitiation complex on eukaryotic genes differently. | in vitro transcription was reconstituted with hela cell transcription factors and rna polymerase ii, which were essentially free from dna topoisomerase activities. dna templates with defined negative superhelical densities were tested for transcription activity. transcription of the bombyx mori fibroin gene increases and plateaus from templates of increasing superhelicity, and transcription from the adenovirus 2 major late promoter rises and then falls, while transcription of the drosophila hsp7 ... | 1991 | 1647522 |
| isolation of cdna clones coding for humoral lectin of silkworm, bombyx mori, larvae. | in the hemolymph of the silkworm, bombyx mori, lectin with hemagglutinating activity against sheep red blood cells increases at larval-larval ecdysis and at spinning stage (suzuki and natori, 1983) and is induced by infection with cytoplasmic polyhedrosis virus. a bombyx lectin polypeptide with molecular weight approx 280k is responsible for hemagglutinating activity, since antiserum raised against this polypeptide inhibited hemagglutinating activity. the site of synthesis of bombyx lectin was d ... | 1992 | 1541849 |
| the design, expression, and characterization of human insulin-like growth factor ii (igf-ii) mutants specific for either the igf-ii/cation-independent mannose 6-phosphate receptor or igf-i receptor. | five mutants of recombinant insulin-like growth factor-ii (rigf-ii) that bound with high affinity to either the igf-ii/cation-independent mannose 6-phosphate (igf-ii/cim6-p) or the igf-i receptor were prepared by site-directed mutagenic procedures, expressed as fusion proteins in the larva of bombyx mori or escherichia coli, purified to homogeneity, renatured, and characterized in terms of their receptor binding affinities and specificities as well as their biological activities. class i mutants ... | 1991 | 1657932 |
| basic conditions for the drug selection and transient gene expression in the cultured cell line of bombyx mori. | we established basic conditions for transient gene expression and selection of antibiotics in the cultured cell line of silkworm, bombyx mori, by use of the promoter of the heat shock protein (hsp70) gene of drosophila melanogaster. the control promoter (hsp70) promoted the expression of chloramphenicol acetyltransferase (cat) gene ligated at the downstream, dependent on the orientation of the promoter in the silkworm cell. the cell line is able to be supplied for the promoter assay of the silkw ... | 1992 | 1483968 |
| primary structure of alanyl-trna synthetase and the regulation of its mrna levels in bombyx mori. | cdna clones encoding bombyx mori alanyl-trna synthetase were isolated from a library in lambda gt11 using antibody, synthetic oligonucleotides, and a characterized cdna as probes. analysis of the sequence revealed significant homology between the b. mori and escherichia coli alanyl-trna synthetases, particularly in their amino-terminal domains. northern blot analysis indicated that the mrna for alanyl-trna synthetase is 3.8 kilobase pairs in mrna isolated from posterior silk gland, middle silk g ... | 1990 | 1701172 |
| formation of open and elongating transcription complexes by rna polymerase iii. | the saccharomyces cerevisiae transcription factors (tf) iiib and iiic assemble onto their respective dna-binding sites on the sup4 trna(tyr) gene at 0 degrees c. rna polymerase iii specifically associates at 0 degrees c with this tfiiic-tfiiib-dna complex to form a stable "closed" promoter complex in which the dna surrounding the transcriptional start retains its duplex form. promoter "opening" is a temperature-dependent and readily reversible process that involves up to 22 unwound base-pairs of ... | 1992 | 1619662 |
| attacin, an antibacterial protein from hyalophora cecropia, inhibits synthesis of outer membrane proteins in escherichia coli by interfering with omp gene transcription. | attacins are antibacterial proteins synthesized by pupae of the giant silk moth, hyalophora cecropia, in response to a bacterial infection. in this report we show that the previously described, attacin-induced alteration in the structure and the permeability of the outer membrane of escherichia coli is associated with a specific inhibition of the synthesis of several outer membrane proteins, including ompc, ompf, ompa, and lamb. the inhibition is expressed as a reduction in the steady-state mrna ... | 1991 | 1715318 |
| evolution of retroposons by acquisition or deletion of retrovirus-like genes. | the retroid family consists of all genetic elements that encode a potential reverse transcriptase (rt). members of this family include a diversity of eukaryotic genetic elements (viruses, transposable elements, organelle introns, and plasmids) and the retrons of prokaryotes. some retroid elements have, in addition to the rt gene, other genes in common with the retroviruses. on the basis of rt sequence similarity, the retroposon group is defined as the eukaryotic long interspersed nuclear element ... | 1991 | 1663570 |
| isolation and primary structure of a novel pheromonotropic neuropeptide structurally related to leucopyrokinin from the armyworm larvae, pseudaletia separata. | a novel pheromonotropic neuropeptide has been isolated from a head extract of the armyworm larvae, pseudaletia separata, by a seven step purification procedure using an in vivo assay with decapitated female moths of bombyx mori. amino acid sequence analysis and comparison with synthetic peptides established the primary structure of the peptide, termed pseudaletia pheromonotropin (pss pt), as h-lys-leu-ser-tyr-asp-asp-lys-val-phe-glu-asn-val-glu-phe-thr-pro-arg-le u-nh2. pss pt is structurally re ... | 1992 | 1734867 |
| comparison of several promoters and polyadenylation signals for use in heterologous gene expression in cultured drosophila cells. | we have directly compared the ability of four promoters and three polyadenylation (poly(a)) signals to direct heterologous gene expression in stably transfected drosophila melanogaster s2 cells. we compared two constitutive drosophila promoters, the actin 5c distal promoter and the alpha 1-tubulin promoter, with the tightly regulated drosophila metallothionein (mtn) promoter and the bombyx mori fibroin promoter. we find that the actin 5c and induced mtn promoters generate comparable high levels ... | 1991 | 1656386 |
| nucleotide sequence of the gene coding for p40, an occluded virion-specific polypeptide of bombyx mori nuclear polyhedrosis virus. | | 1991 | 1783783 |
| [pharmacological studies on bombyx mori l]. | the toxicity of the bombyx mori extract is very low. it can promote the growth of under-aged male mice, and increase markedly the weight of the prostate glands, seminal vesicles and preputial glands in castrated rats of mice. the results of the experiments have shown that bombyx mori has androgen-like action. | 1991 | 1786102 |
| cloning and expression of the lepidopteran toxin produced by bacillus thuringiensis var sotto in escherichia coli. | during sporulation, bacillus thuringiensis var. sotto produces a parasporal crystalline protein which is toxic for the silk-worm, bombyx mori and the cotton leaf worm, spodoptera littoralis. the gene coding this crystal protein is present in a single plasmid. the plasmid dna was isolated, purified and physically mapped using restriction endonuclease enzymes (r.e.). the gene coding the delta-endotoxin was inserted into escherichia coli-jm103, using cloning vector puc8. transformed e. coli cells w ... | 1991 | 1791407 |
| high-level expression and characterization of hepatitis b virus surface antigen in silkworm using a baculovirus vector. | the bombyx mori nuclear polyhedrosis virus (bmnpv) in the silkworm was used successfully for mass production of biologically active foreign genes under the control of the polyhedrin promoter. this system was adapted for the production of large amounts of hepatitis b virus surface antigens (hbsag). the dna fragments coded for the middle protein, which is composed of the s protein with the pre-s2 region, were cloned, the signal protein gene of beta-ifn was added, and both were inserted into a clon ... | 1991 | 1816252 |
| in vitro propagation of nosema locustae using fat body cell line derived from mythimna convecta (lepidoptera: noctuidae). | nosema locustae, a microsporidian parasite of locusts and grasshoppers, was successfully propagated in a fat body cell line from mythimna convecta (bpmnu-myco-1). the fat body cells were grown in mgm-448 medium supplemented with 5% fetal bovine serum and 3% bombyx mori serum at 25 degrees c. cultures were inoculated with nosema spores and agitated for 2 min. infection appeared 3 days post-inoculation and by 7th day, some cells were filled with spores. at the 15th day post-inoculation, 32% of the ... | 1991 | 1818221 |
| structural analysis of a mature hst-1 protein with transforming growth factor activity. | a recombinant hst-1 protein produced in silkworm cells by a recombinant baculovirus, previously shown to be a potent mitogen for nih3t3 cells and human endothelial cells, also stimulated anchorage-independent growth of nrk-49f cells. amino acid sequence analysis revealed that the amino-terminal sequence with 58 amino acids was cleaved off in silkworm cells. these results indicated that the mature hst-1 protein consisting of 148 amino acids had transforming growth factor activity. | 1991 | 1824921 |
| studies of l-canavanine incorporation into insectan lysozyme. | l-canavanine is incorporated into the lysozyme synthesized, in response to administration of bacterial cell wall materials, by canavanine-treated larvae of the tobacco hornworm manduca sexta (sphingidae). maximum canavanine incorporation into m. sexta lysozyme occurs when the larvae are provided 1 mg of canavanine g-1 fresh body weight. analysis of canavanine-containing lysozyme purified from these insects reveals that 21% of the arginine residues are replaced by canavanine; this residue substit ... | 1991 | 1874726 |
| enrichment of biologically active u1 small nuclear rnas by ion-exchange high-performance liquid chromatography. | the use of ion-exchange high-performance liquid chromatography in conjunction with preparative electrophoresis to facilitate the purification of biologically active snrnas is described. separation of total nuclear rna from a bombyx mori cell line was done with a bio-rad ma7 plasmid column in a hrlc 500 system. individual fractions were subjected to electrophoresis through 14% polyacrylamide gels for identification. high levels of u1 rna were confirmed by northern analysis with a human u1 probe. ... | 1991 | 1894726 |
| isolation and structural characterization of an insulin-related molecule, a predominant neuropeptide from locusta migratoria. | neurohaemal lobes of corpora cardiaca of locusta migratoria are an established storage site for neurohormones produced by the neurosecretory cells of the brain. as previously reported [hietter, h., van dorsselaer, a., green, b., denoroy, l., hoffmann, j.a. & luu, b. (1990) eur. j. biochem. 187, 241-247], the isolation and characterization of a novel 5-kda peptide from these lobes served as the basis for oligonucleotide screening of cdna libraries prepared from poly(a) rna from neurosecretory cel ... | 1991 | 1935945 |
| homeodomain binding sites in the 5' flanking region of the bombyx mori silk fibroin light-chain gene. | multiple a + t-rich stretches in the 5' flanking region of the bombyx mori fibroin light-chain gene have been shown to bind two drosophila homeodomain proteins, eve (even-skipped) and zen (zerknüllt), with high affinities. some of these sites fall into a class that has the established consensus sequence of the binding sites (tcaattaaat) for a diverse group of drosophila homeodomain proteins, while others are quite heterogenous except that they all possess a core taat motif. since clusters of hom ... | 1990 | 1972197 |
| x-ray powder pattern analysis of cytoplasmic polyhedrosis virus inclusion bodies. | cytoplasmic polyhedrosis virus is an insect reovirus which is occluded in crystalline inclusion bodies that form in the mid-gut of certain insects. inclusion bodies of cytoplasmic polyhedrosis virus from bombyx mori were isolated and purified. these crystalline bodies, about 1-3 microns in linear size, were compacted in a capillary tube while immersed in buffer. x-ray diffraction photographs showed powder rings, extending to 8.2 a resolution, which could be indexed with a cell measuring a = b = ... | 1991 | 1984644 |
| cloning and sequence analysis of membrane-bound alkaline phosphatase cdna of the silkworm, bombyx mori. | the nucleotide sequence (1974 bp) of cdna coding for membrane-bound alkaline phosphatases (m-alp) of bombyx mori was isolated. the cdna clone contained an open reading frame encoding a polypeptide (547 amino acids), which contains a hydrophobic signal peptide of 36 amino acids and the mature protein of 511 amino acids (mr = 56,163). we found a highly hydrophobic domain presumed to be a membrane anchoring region at the c-terminus. comparing analysis between bombyx m-alp and mammalian and escheric ... | 1991 | 1756175 |
| characterization of v-sis protein expressed in silkworm larvae using the bombyx mori nuclear polyhedrosis virus vector. | the v-sis oncogene of simian sarcoma virus encodes a protein which is homologous to the human platelet-derived growth factor b-chain. the v-sis protein undergoes a series of processing steps including dimer formation and proteolytic digestion to generate several molecular sizes of the protein. two of these v-sis proteins were expressed alone or as polyhedrin-sis fusion proteins using the bombyx mori nuclear polyhedrosis virus vector. the polyhedrin-sis fusion proteins contained a collagenase-sen ... | 1991 | 2016272 |
| mass spectrometric determination of glycosylation sites and oligosaccharide composition of insect-expressed mouse interleukin-3. | the primary structure of baculovirus-expressed mouse interleukin-3 produced in infected bombyx mori larvae was characterized by liquid secondary ion mass spectrometry and 252cf-plasma desorption mass spectrometry in combination with selected protein microchemical reactions. interleukin-3 was found to consist of at least two glycoprotein species of ca. 17,000 dalton. characterization of tryptic and s. aureus v8 protease peptides by edman degradation combined with plasma desorption mass spectromet ... | 1991 | 2026707 |
| alanyl-trna synthetase from escherichia coli, bombyx mori and ratus ratus. existence of common structural features. | alanyl-trna synthetase from escherichia coli, bombyx mori and rat were examined with respect to the following functional and structural properties: the effect of substrates on sensitivity to proteolysis, secondary structure as determined by circular dichroism, amino acid composition and, in the case of the rat and insect enzymes, partial amino acid sequence determination on a 60-kda c-terminal tryptic fragment. digestion of the enzyme from all three sources with trypsin resulted in significant d ... | 1991 | 2040280 |
| host range expansion by recombination of the baculoviruses bombyx mori nuclear polyhedrosis virus and autographa californica nuclear polyhedrosis virus. | the mechanisms of host specificity of nuclear polyhedrosis viruses (npvs) (baculoviridae) were analyzed after coinfection of bombyx mori npv (bmnpv) and one of four distinct groups of spodoptera litura npv (slnpv), including an autographa californica npv (acnpv) variant (s. maeda, y. mukohara, and a. kondo, j. gen. virol. 71:2631-2639, 1990), into various lepidopteran cell lines. replication of bmnpv in nonpermissive cells (tn-386, sf-21, and cls-79) was induced by coinfection with acnpv but not ... | 1991 | 2041087 |
| expression of htlv-i envelope protein fused to hydrophobic amino-terminal peptide of baculovirus polyhedrin in insect cells and its application for serological assays. | the envelope of human t-cell leukemia virus type i (htlv-i) consists of two glycoproteins gp46 and p20e. recombinant envelope proteins were produced by using an expression vector derived from insect baculovirus, bombyx mori nuclear polyhedrosis virus. polyhedrin fusion proteins c182, n147, and n287 contained whole region p20e, c-terminal half of gp46, and almost whole region gp46, respectively. n147 and n287 were suggested to be processed forms resulting from internal cleavage by cellular enzyme ... | 1990 | 2078412 |
| transient expression of chimeric cat genes injected into early embryos of the domesticated silkworm bombyx mori. | in order to establish a transient expression system for genes introduced into early embryos of the silkworm, bombyx mori, we tested various promoters ligated with cat reporter genes. the embryos into which we injected supercoiled plasmid dna of pfb(-860/+10)cat containing the bombyx fibroin promoter region ligated to the cat gene showed a reasonably high cat activity beginning around 30 h after oviposition. this high activity was observed only when the plasmid was injected before termination of ... | 1990 | 2088426 |