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entomogenous microorganisms associated with the japanese beetle, popillia japonica, in eastern north carolina. 1978103973
physiology of sporeforming bacteria associated with insects: metabolism of bacillus popilliae grown in third-instar popillia japonica newman larvae.the timing and relative participation of concurrent pathways of carbohydrate metabolism as well as the extent of terminal respiratory activity were determined by radiorespirometry with 14-c substrates and by enzyme assays for vegetative and sporulating cells of the bacterium bacillus popilliae cultured in whole, intact popillia japonica (japanese beetle) larvae. during vegetative proliferation, the pentose phosphate pathway predominates in the bacterial cells with minor involvement of the embden ...19751147615
infection of popillia japonica larvae with heat-activated spores of bacillus popilliae. 19685649242
characterization of larvicidal toxin protein from bacillus thuringiensis serovar japonensis strain buibui specific for scarabaeid beetles.the delta-endo toxin proteins from bacillus thuringiensis which kill the larvae of various scarabaeid beetles such as anomala cuprea, a. rufocuprea and popillia japonica were purified by deae ion exchange chromatography. a protein with a molecular size of 130 kda was purified. during the purification a minor peak was also detected which was estimated to be 67 kda by sds-page. both 130 and 67 kda proteins showed larvicidal activity against a. cuprea. the lethal concentration of the 130 kda protei ...19948200856
sequence relationship of retrotransposable elements r1 and r2 within and between divergent insect species.r1 and r2 are retrotransposable elements that integrate at specific sites in the 28s ribosomal rna (rrna) genes of bombyx mori and drosophila melanogaster. we have previously shown that most insect species contain insertions in their 28s genes at the r1 and/or r2 site. we have sequenced the 3' half of r1 and r2 elements from three additional insect species: the fungus gnat, sciara coprophila (diptera); the japanese beetle, popillia japonica (colleoptera); and the parasitic wasp, nasonia vitripen ...19938383793
susceptibility of the taro beetle, papuana uninodis (coleoptera, scarabaeidae) to two new bacillus popilliae isolates from papuana spp.two morphological types of bacillus popilliae, causal agent of the milky disease, have been isolated from taro beetles (papuana spp, coleoptera: scarabaeidae). b. popilliae from p. woodlarkiana woodlarkiana (papua new guinea) was a type a1 with a small sporangium (4.1 x 1.6 microm) and a large spore (2.1 x 1.4 microm) and parasporal body (1.8 x 1.2 microm) that sometimes overlap. b. popilliae from p. uninodis and p. woodlarkiana laevipennis (solomon islands) was a type b2 with a small sporangium ...199910222178
impact of four insecticides on japanese beetle (coleoptera: scarabaeidae) egg predators and white grubs in turfgrass.field experiments were conducted to measure the effects of four commonly used turfgrass insecticides (isofenphos, diazinon, imidacloprid, halofenozide) on white grubs (coleoptera: scarabaeidae) and ant predators of white grub eggs. ant populations were measured over time with canned tuna, whereas predation by the ants was measured with artificially placed japanese beetle, popillia japonica newman, eggs. the effectiveness of each insecticide at controlling japanese beetle grubs, when applied at d ...200111233105
management of early-instar japanese beetle (coleoptera: searabaeidae) in field-grown nursery crops.numerous field studies were conducted in commercial nurseries in tennessee from 1996 through 1999 to evaluate chemical and biological treatments, application timing and rates, and method of application for control of early instars of japanese beetle, popillia japonica newman. insecticide treatments included bifenthrin, bendiocarb, chlorpyrifos, carbaryl, fipronil, halofenozide, imidacloprid, permethrin, tefluthrin, thiamethoxam, and trichlorfon. biological treatments included entomopathogenic ne ...200111681678
solid state fermentation of broiler litter for production of biocontrol agents.several varieties of heat-sterilized broiler litter with 60% (wet basis, wb) moisture content were substrate in solid-state fermentations to produce biocontrol agents. litter varieties included litter produced by one flock of broilers from medicated and non-medicated controlled rations, and litter produced by two flocks and four flocks on a single application of bedding material from medicated commercial sources. litter preparations were inoculated with monocultures of bacillus thuringiensis ser ...200211848375
nutritional requirements of bacillus popilliae.sylvester, charles j. (michigan state university, east lansing), and ralph n. costilow. nutritional requirements of bacillus popilliae. j. bacteriol. 87:114-119. 1964.-bacillus popilliae grew consistently well in a semisynthetic medium containing a "vitamin-free," salt-free, acid hydrolysate of casein supplemented with dextrose, dl-tryptophan, and thiamine. however, when the casein hydrolysate was replaced with a complete array of amino acids, no growth occurred. the addition of barbituric acid ...196414102843
biological control agents for white grubs (coleoptera: scarabaeidae) in anticipation of the establishment of the japanese beetle in california.we tested biological control agents for the control of 3rd-instar scarab turfgrass pests, both for the masked chafer cyclocephala hirta leconte and the japanese beetle, popillia japonica newman. the former species is endemic in california whereas the latter, although not yet established, constitutes a permanent serious threat to agriculture and horticulture in california. we conducted experiments using c. hirta in california and p. japonica in new jersey. a field trial conducted in 2 different c ...200014658514
floral affinity and benefits of dietary mixing with flowers for a polyphagous scarab, popillia japonica newman.many generalist herbivores, especially adult beetles, are facultative florivores, feeding on leaves but readily accepting floral tissues when available. we speculated that day-flying beetles with high energetic requirements would benefit from dietary mixing with nutrient-rich flower tissues and favor them during foraging. we tested that "floral affinity hypothesis" with popillia japonica, a day-active ruteline scarab that feeds intermittently throughout its adult life on multiple plant species. ...200415146324
efficacy of piper (piperaceae) extracts for control of common home and garden insect pests.extracts from three species of the plant family piperaceae, piper nigrum [l.], piper guineense [schum & thonn, and piper tuberculatum [jacq.], were tested for efficacy against insects from five orders. all three species contain isobutyl amides, plant secondary compounds that act as neurotoxins in insects. these materials are considered safe to mammals because piper spp. were used for centuries for spice and medicinal purposes. when 24-h p. nigrum lc50 values were compared between common insect p ...200415384353
evaluation of hydrophobic and hydrophilic kaolin particle films for peach crop, arthropod and disease management.hydrophobic and/or hydrophilic kaolin particle film treatments to peach (prunus persica (l) batsch) trees were evaluated for crop and pest management capabilities in six studies from 1997 to 2000. unsprayed control and standard treatments, the latter consisting of a commercial pesticide program, were included for comparison. treatments in initial studies were applied via handgun, which resulted in a uniform and heavy deposit of kaolin after the first application. in contrast, treatments in subse ...200515593071
uptake of bt endotoxins by nontarget herbivores and higher order arthropod predators: molecular evidence from a transgenic corn agroecosystem.the planting of transgenic crops expressing bacillus thuringiensis endotoxins is widespread throughout the world; the prolific increase in their application exposes nontarget organisms to toxins designed to control pests. to date, studies have focused upon the effects of bt endotoxins on specific herbivores and detritivores, without consideration of their persistence within arthropod food webs. here, we report the first quantitative field evaluation of levels of bt endotoxin within nontarget her ...200516029480
characterization of bacillus thuringiensis strain bt185 toxic to the asian cockchafer: holotrichia parallela.a new bacillus thuringiensis strain, bt185, was isolated from hebei soil samples in china. observations after transmission electron microscopy found that the strain produced spherical parasporal inclusions similar to that of the b. thuringiensis subsp. japonensis buibui strain, which showed toxicity to both anomala corpulenta and popillia japonica. the plasmid profile seen on an agarose gel revealed that bt185 contained six large bands of 191 kb, 161 kb, 104 kb, 84 kb, 56 kb, and 37 kb. sodium d ...200616775781
impact of japanese beetle (coleoptera: scarabaeidae) feeding on seashore paspalum.ten cultivars of seashore paspalum, paspalum vaginatum swartz, were compared for their response to japanese beetle, popillia japonica newman, larval root feeding. cultivars of bermuda grass, cynodon sp., and zoysiagrass, zoysia sp., also were included for comparison. turf grown in pots in the greenhouse was infested with second and third instars in this 2-yr study. grub survival and weight gain, foliar growth, and root loss were compared among turfgrass species and cultivars. few species-related ...200617066801
relationship between the successful infection by entomopathogenic nematodes and the host immune response.reproduction of entomopathogenic nematodes requires that they escape recognition by a host's immune system or that they have mechanisms to escape encapsulation and melanization. we investigated the immune responses of larvae for the greater wax moth (galleria mellonella), tobacco hornworm (manduca sexta), japanese beetle (popillia japonica), northern masked chafer (cyclocephala borealis), oriental beetle (exomala orientalis) and adult house crickets (acheta domesticus), challenged with infective ...200717275827
residual effects of imidacloprid on japanese beetle (coleoptera: scarabaeidae) oviposition, egg hatch, and larval viability in turfgrass.preventive control of turf-infesting scarabaeid grubs by neonicotinoid insecticides is presumed to mainly result from residues killing first instars in the soil. the extent to which sublethal behavioral effects or intoxication of other life stages contribute to such control is poorly known. we tested whether japanese beetle, popillia japonica newman, females lay fewer eggs in turf treated with imidacloprid (merit 75 wp) or an imidacloprid-bifenthrin combination (allectus gc sc), and whether expo ...200717461068
the phylogenetic position of ovavesicula popilliae (microsporidia) and its relationship to antonospora and paranosema based on small subunit rdna analysis.comparative small subunit rdna sequence analyses, indicate that ovavesicula popilliae, a microsporidian parasite of the japanese beetle, popillia japonica, represents a distant sister group to paranosema and antonospora. these three genera represent a second major group (the nosema/vairimorpha clade representing the first) of microsopridia which infect terrestrial insects, suggesting independent origins for both groups. phylogenetic analyses of ovavesicula and other microsporidia having a multi- ...200717599347
susceptibility of four species of turfgrass-infesting scarabs (coleoptera: scarabaeidae) to bacillus thuringiensis serovar japonensis strain buibui.bacillus thuringiensis serovar japonensis strain buibui experimental formulations containing cry8cal delta-endotoxin were evaluated in the field for the control of oriental beetle, anomala orientalis waterhouse; japanese beetle, popillia japonica newman; asiatic garden beetle, maladera castanea (arrow); and european chafer, rhizotrogus majalis (razoumowsky) larvae infesting turfgrasses. in 17 fall applications, control of a. orientalis and p. japonica was achieved at rates as low as 100 g of tox ...200717972638
discovery of a novel bacillus thuringiensis cry8d protein and the unique toxicity of the cry8d-class proteins against scarab beetles.a novel cry gene, cry8db, highly toxic to scarab beetles such as the japanese beetle, popillia japonica newman, was cloned from an isolate of bacillus thuringiensis(bt), bbt2-5. the cry8db gene has 3525bp nucleotides and codes for a protein of 1174 amino acid residues. the protein, cry8db, has typical bt characteristics such as the 8-block, conserved sequences and the three-domain 3d toxin structure as defined with cry3aa. when the amino acid sequence of cry8db was compared with that of cry8da w ...200818614174
quantitative resistance traits and suitability of woody plant species for a polyphagous scarab, popillia japonica newman.the japanese beetle, popillia japonica newman, has an unusually broad host range among deciduous woody plants, yet it feeds only sparingly, or not at all, on certain species in the field. we evaluated beetles' preference, survival over time and fecundity on eight woody plant species historically rated as susceptible or resistant and, after verifying those ratings, tested whether resistance is correlated with so-called quantitative defense traits including leaf toughness, low nutrient content (wa ...200819161699
invasive japanese beetles facilitate aggregation and injury by a native scarab pest of ripening fruits.invasive species' facilitation, or benefiting, of native species is rarely considered in biological invasion literature but could have serious economic consequences should a non-native herbivore facilitate injury by a native pest of high-value crops. japanese beetle (jb), popillia japonica, a polyphagous scarab, facilitates feeding by the obligate fruit-feeding native green june beetle (gjb), cotinis nitida, by biting into intact grape berries that gjb, which has blunt spatulate mandibles, is ot ...200919234133
field evaluation of essential oils for reducing attraction by the japanese beetle (coleoptera: scarabaeidae).forty-one plant essential oils were tested under field conditions for the ability to reduce the attraction of adult japanese beetles, popillia japonica newman (coleoptera: scarabaeidae), to attractant-baited or nonbaited traps. treatments applied to a yellow and green japanese beetle trap included a nonbaited trap, essential oil alone, a japanese beetle commercial attractant (phenethyl proprionate:eugenol:geraniol, 3:7:3 by volume) (peg), and an essential oil plus peg attractant. eight of the 41 ...200919736768
ovipositional preferences of the japanese beetle (coleoptera: scarabaeidae) among warm- and cool-season turfgrass species.japanese beetles, popillia japonica newman (coleoptera: scarabaeidae), were evaluated for ovipositional preferences among four turfgrasses common in northwestern arkansas. choice assays revealed females preferred to oviposit in tall fescue (festuca arundinacea schreb.) and zoysiagrass (zoysia japonica steud.), and that they avoided oviposition in common bermudagrass (cynodon dactylon [l.] pers.) and hybrid bermudagrass (c. dactylon x c. transvaalensis pers.). significantly fewer eggs were ovipos ...200920069848
impact of elevated levels of atmospheric co2 and herbivory on flavonoids of soybean (glycine max linnaeus).atmospheric levels of carbon dioxide (co2) have been increasing steadily over the last century. plants grown under elevated co2 conditions experience physiological changes, particularly in phytochemical content, that can influence their suitability as food for insects. flavonoids are important plant defense compounds and antioxidants that can have a large effect on leaf palatability and herbivore longevity. in this study, flavonoid content was examined in foliage of soybean (glycine max linnaeus ...201020077130
identification of qtl in soybean underlying resistance to herbivory by japanese beetles (popillia japonica, newman).soybean [glycine max (l.) merr.] was one of the most important legume crops in the world in 2010. japanese beetles (jb; popillia japonica, newman) in the us were an introduced and potentially damaging insect pest for soybean. jbs are likely to spread across the us if global warming occurs. resistance to jb in soybean was previously reported only in plant introductions. the aims here were to identify loci underlying resistance to jb herbivory in recombinant inbred lines (rils) derived from the cr ...201020458460
intramolecular proteolytic nicking and binding of bacillus thuringiensis cry8da toxin in bbmvs of japanese beetle.bacillus thuringiensis (bt) cry8d insecticidal proteins are unique among cry8 family proteins in terms of its insecticidal activity against adult scarab beetles, such as japanese beetle (popillia japonica newman). from the sequence homology with other bt cry proteins especially those active against beetles, such as cry3aa whose 3d structure is available, the structure of the cry8d protein has been predicted to be a typical three-domain cry protein type. in addition, the activation process of cry ...201020655921
olfactory preferences of popillia japonica, vanessa cardui, and aphis glycines for glycine max grown under elevated co(2).levels of atmospheric co(2) have been increasing steadily over the last century and are projected to increase even more dramatically in the future. soybeans (glycine max l.) grown under elevated levels of co(2) have larger herbivore populations than soybeans grown under ambient levels of co(2). increased abundance could reflect the fact that these herbivores are drawn in by increased amounts of volatiles or changes in the composition of volatiles released by plants grown under elevated co(2) co ...201022127180
incidence of turf-damaging white grubs (coleoptera: scarabaeidae) and associated pathogens and parasitoids on kentucky golf courses.root-feeding grubs (coleoptera: scarabaeidae) were sampled from damaged areas of 61 irrigated roughs on 32 kentucky golf courses to determine species composition and natural enemy incidence, the first such survey in the united states' transitional turfgrass climatic zone. masked chafers (cyclocephala lurida bland and c. borealis arrow) and japanese beetle (popillia japonica newman) accounted for ?73 and 26% of grubs found in an autumn survey, with cyclocephala spp. predominating at most sites, ...201022182549
identification of a bacillus thuringiensis cry8da toxin-binding glucosidase from the adult japanese beetle, popillia japonica.cry8da from bacillus thuringiensis galleriae sds-502 has insecticidal activity against both the larvae and adult japanese beetle (popillia japonica newman). the receptor determines the specificity of the insecticidal activity of cry proteins and hence, in order to reveal the mode of action of cry toxin, receptor identification is a necessary step. however, a receptor for cry8-type toxin has not been identified in the scarabaeidae family of insects. therefore, we aimed to identify the receptor of ...201323541518
differences in immune defense evasion of selected inbred lines of heterorhabditis bacteriophora in two white grub species.we determined virulence of seven heterorhabditis bacteriophora strain gps11 inbred lines possessing superior infective juvenile longevity, and heat and ultra violet radiation tolerance against white grubs popillia japonica and cyclocephala borealis. at 1 and 2 weeks after treatment, inbred line a2 was significantly more virulent towards p. japonica compared to the parent strain gps11 and inbred lines a7, a8, a12 and a21; and line a2 caused significantly higher c. borealis mortality than lines a6 ...201226466530
susceptibility of the adult japanese beetle, popillia japonica to entomopathogenic nematodes.to build upon prior research demonstrating the potential of entomopathogenic nematode dissemination by infected adult japanese beetle, popillia japonica, we evaluated susceptibility of the adult beetles to 20 strains of steinernema and heterorhabditis under laboratory conditions. the nematodes were applied at a rate of 10,000 infective juveniles per 10 adult beetles in 148 ml plastic cups containing autoclaved sand and sassafras leaves as a source of food for the beetles. all strains infected th ...201123431080
attraction of four entomopathogenic nematodes to four white grub species.to better understand the differences in the efficacy of entomopathogenic nematode species against white grub species, we are studying the various steps of the infection process of entomopathogenic nematodes into different white grub species using nematode species/strains with particular promise as white grub control agents. in this study we compared the attraction of the entomopathogenic nematodes steinernema scarabaei (amk001 strain), steinernema glaseri (nc1 strain), heterorhabditis zealandica ...200818597774
early timing and new combinations to increase the efficacy of neonicotinoid-entomopathogenic nematode (rhabditida: heterorhabditidae) combinations against white grubs (coleoptera: scarabaeidae).an investigation was carried out to determine whether new neonicotinoid-nematode combinations and earlier applications against younger larval stages could increase the efficacy of synergistic neonicotinoid-entomopathogenic nematode combinations against white grubs.200818260065
effect of soil type on infectivity and persistence of the entomopathogenic nematodes steinernema scarabaei, steinernema glaseri, heterorhabditis zealandica, and heterorhabditis bacteriophora.we tested the effect of soil type on the performance of the entomopathogenic pathogenic nematodes steinernema scarabaei, steinernema glaseri, heterorhabditis zealandica, and heterorhabditis bacteriophora. soil types used were loamy sand, sandy loam, loam, silt loam, clay loam, acidic sand, and a highly organic potting mix. infectivity was tested by exposing third-instar anomala orientalis or popillia japonica to nematodes in laboratory and greenhouse experiments and determining nematode establis ...200616563427
effect of white grub developmental stage on susceptibility to entomopathogenic nematodes.the pathogenicity of the entomopathogenic nematodes heterorhabditis bacteriophora poinar and steinernema scarabaei stock & koppenhöfer against different developmental stages of the japanese beetle, popillia japonica newman, and the oriental beetle, anomala (=exomala) orientalis waterhouse, were studied under laboratory conditions. the efficacy of s. scarabaei did not differ between second and third instars in p. japonica or a. orientalis or between small (young) and large (older) third instars i ...200415666735
variations in immune response of popillia japonica and acheta domesticus to heterorhabditis bacteriophora and steinernema species.the infectivities of steinernema carpocapsae, s. glaseri, s. scapterisci, and heterorhabditis bacteriophora to japanese beetle larvae, popillia japonica, and house cricket adults, acheta domesticus, were compared using external exposure and hemocoelic injection. only h. bacteriophora and s. glaseri caused high p. japonica mortality after external exposure. when nematodes were injected, p. japonica had a strong encapsulation and melanization response to all species except s. glaseri. heterorhabdi ...199419279863
effectiveness of steinernema spp. and heterorhabditis bacteriophora against popillia japonica in the azores.steinernema carpocapsae (breton strain), s. glaseri, and heterorhabditis bacteriophora were evaluated for their potential to control immature stages of the japanese beetle, popillia japonica, on terceira island (the azores). in bioassays carried out at temperatures higher than 15 c, s. glaseri and h. bacteriophora caused 100% mortality of larvae, whereas s. carpocapsae caused 56% larval mortality. at temperatures slightly below 15 c, only s. glaseri remained effective. in field plots, in septemb ...199319279799
survey of pathogens and parasitoids of popillia japonica (coleoptera: scarabaeidae) in northwest arkansas.the impact of pathogens and parasitoids on the recently established population of popillia japonica newman in northwest arkansas has been unknown. in this study, we quantified the prevalence of natural enemies: stictospora villani hays, ovavesicula popilliae andreadis and hanula, paenibacillus spp. (dingman), nematodes and parasitic diptera and hymenoptera in third instar and adult populations in 2010 and 2011. s. villani was found in 38.4% and 35.5% of larvae in 2010 and 2011, respectively. s. ...201222721914
chlorpyrifos immersion to eliminate third instars of japanese beetle (coleoptera: scarabaeidae) in balled and burlapped trees and subsequent treatment effects on red maple.this study examined chlorpyrifos immersion of balled and burlapped (b&b) nursery trees for elimination of third instars of japanese beetle, popillia japonica newman (coleoptera: scarabaeidae), and for phytotoxicity on red maple, acer rubrum l. trees were harvested as 45- and 60-cm-diameter b&b and immersed in chlorpyrifos at u.s. domestic japanese beetle harmonization plan rate (0.24 kg active ingredient [ai/100 liters) or lower rates of 0.015, 0.03, 0.06, and 0.12 kg (ai)/100 liters. the 0.03, ...200717461051
acute toxicity of plant essential oils to scarab larvae (coleoptera: scarabaeidae) and their analysis by gas chromatography-mass spectrometry.larvae of scarab beetles (coleoptera: scarabaeidae) are important contaminant and root-herbivore pests of ornamental crops. to develop alternatives to conventional insecticides, 24 plant-based essential oils were tested for their acute toxicity against third instars of the japanese beetle popillia japonica newman, european chafer rhizotrogus majalis (razoumowsky), oriental beetle anomala orientalis (waterhouse), and northern masked chafer cyclocephala borealis arrow. diluted solutions were topic ...201323448028
toxicity of botanical formulations to nursery-infesting white grubs (coleoptera: scarabaeidae).the toxicity of eight botanically based biopesticides was evaluated against third instars of the scarab larvae (coleoptera: scarabaeidae) popillia japonica newman, rhizotrogus majalis (razoumowsky), anomala orientalis waterhouse, and cyclocephala borealis arrow. soil dip bioassays were used to obtain concentration-mortality data 7 d after treatment of larvae, leading to the calculation of lc50 and lc90 values. a wide range in lc50 and lc90 values were exhibited among the formulations. the produc ...200919253649
knockdown, residual, and antifeedant activity of pyrethroids and home landscape bioinsecticides against japanese beetles (coleoptera: scarabaeidae) on linden foliage.residual toxicity and leaf protection capability of five pyrethroids, professional and home garden azadirachtin formulations, and six other bioinsecticides for the home landscape were evaluated against the japanese beetle, popillia japonica newman (coleoptera: scarabaeidae), on linden, tilia cordata l. capacity of intoxicated beetles to recover and subsequently feed and disperse also was evaluated to provide insight on activity characteristics of the different compounds. intact shoots were spray ...200717461070
evaluating companion planting and non-host masking odors for protecting roses from the japanese beetle (coleoptera: scarabaeidae).effectiveness of companion planting, and use of nonhost masking odors were evaluated under field conditions for protecting roses against the japanese beetle, popillia japonica newman. three reputedly effective companion species, rue (ruta graveolens l.), zonal geranium (pelargonium x hortorum bailey), and garlic chives (allium scheonparum l.) were interplanted with roses in replicated garden plots. numbers of beetles on these roses were compared with rose-only control plots on 6 d during beetle ...200312650348
variation in the laboratory susceptibility of turf-infesting white grubs (coleoptera: scarabaeidae) to biological, biorational and chemical control products.white grubs are the most widespread and damaging pests in turfgrass habitats of the northeast usa, and their management is highly dependent on chemical pesticides. because this complex includes at least eight species, opportunities for pest management would be enhanced by understanding how susceptibility to control products varies across taxa. the objective of this laboratory study was to measure variation in the susceptibility of four species to 18 biological, biorational and chemical insectici ...201019742452
phytoecdysteroids as antifeedants towards several beetles that include polyphagous and monophagous feeding guilds.plants are thought to produce ecdysteroids as a means of protection from insect herbivores. some insects will not feed on plants containing high amounts of phytoecdysteroids, and this response could be limited to monophagous and oligophagous insects. the aim of this study was to determine whether phytoecdysteroids could inhibit feeding in several species of beetles that range from monophagous to polyphagous.201627976533
anthropogenic increase in carbon dioxide compromises plant defense against invasive insects.elevated levels of atmospheric carbon dioxide (co2), a consequence of anthropogenic global change, can profoundly affect the interactions between crop plants and insect pests and may promote yet another form of global change: the rapid establishment of invasive species. elevated co2 increased the susceptibility of soybean plants grown under field conditions to the invasive japanese beetle (popillia japonica) and to a variant of western corn rootworm (diabrotica virgifera virgifera) resistant to ...200818375762
field resistance of two soybean germplasm lines, hc95-15mb and hc95-24mb, against bean leaf beetle (coleoptera: chrysomelidae), western corn rootworm (coleoptera: chrysomelidae), and japanese beetles (coleoptera: scarabaidae).two recently released, mexican bean beetle, epilachna varivestis, mulsant, resistant soybean, glycine max (l.) merrill, germplasm lines, hc95-15mb and hc95-24mb, were examined for foliar and pod feeding resistance to adult bean leaf beetles, cerotoma trifurcata (forster), western corn rootworms, diabrotica virgifera virgifera leconte, and japanese beetles, popillia japonica newman. both lines were planted along with a susceptible control cultivar in 18 by 30-m plots and separate 0.8-ha size fiel ...200111777070
relative resistance or susceptibility of maple (acer) species, hybrids and cultivars to six arthropod pests of production nurseries.maples (acer spp.) in production nurseries are vulnerable to numerous arthropod pests that can stunt or even kill the young trees. seventeen cultivars representing various acer species and hybrids were evaluated for extent of infestation or injury by shoot and trunk borers (proteoteras aesculana, chrysobothris femorata), potato leafhopper (empoasca fabae), japanese beetle (popillia japonica), maple spider mite (oligonychus aceris) and calico scale (eulecanium cerasorum). evaluations were done in ...201322865707
soil application of neonicotinoid insecticides for control of insect pests in wine grape vineyards.soil application of systemic neonicotinoid insecticides can provide opportunities for long-term control of insect pests in vineyards, with minimal risk of pesticide drift or worker exposure. this study compared the effectiveness of neonicotinoid insecticides applied via irrigation injection on key early-season and mid-season insect pests of vineyards in the eastern united states.201222290809
influence of culture method on steinernema glaseri lipids.entomopathogenic nematodes can be mass produced in artificial media for use as biological insecticides. nematode in vitro media have been primarily developed on the basis of yield without fully considering nematode nutritional requirements. we investigated the quality and quantity of lipids in the entomopathogenic nematode steinernema glaseri when grown in vivo in popillia japonica (a natural host), galleria mellonella (a factitious host), and in solid and liquid media. nematode yield (infective ...19989576490
comparison of scarab grub populations and associated pathogens and parasitoids in warm- or cool-season grasses used on transitional zone golf courses.seven different turfgrass species or mixes used on golf courses in the united states' transitional climatic zone were maintained as randomized and replicated plots in separate stands mowed at fairway (1.6 cm) or rough (6.4 cm) cutting heights and sampled in autumn to assess the density and species composition of scarab grubs; incidence of disease and parasitism thereof; and extent of turf damage from foraging insectivorous skunks, mephitis mephitis. influence of grass species on parasitism by sp ...201222928312
revision of the genus stictospora and description of stictospora villani, n. sp. (apicomplexa: eugregarinida: actinocephalidae) from larvae of the japanese beetle, popillia japonica (coleoptera: scarabaeidae), in michigan.stictospora villani n. sp. is described from larvae of the japanese beetle popillia japonica (coleoptera: scarabaeidae) from southern michigan. stictospora villani is distinguished from known species of the genus by differences in gamont size and by structural details of the epimerite. in general, s. villani is smaller than other known species of the genus and possesses an epimerite with a margin of 20-24 sympetalous, pendulate, narrowly to very narrowly elliptoid lamina. previously described sp ...200415715242
the susceptibility of parental and hybrid willows to plant enemies under contrasting soil nutrient conditions.we conducted an experimental study of the effects of nutrient addition on the susceptibility of two species of willows (salix eriocephala and s. sericea) and their hybrid to a pathogen and several herbivores. we hypothesized that the relative susceptibility of parental and hybrid willows would depend upon soil nutrient availability and vary among plant enemies. using potted plants in a common garden, we found that s. eriocephala was significantly more susceptible to attack by a fungal rust (mela ...199728307538
impact of elevated co2 and increased temperature on japanese beetle herbivory.to examine how the major elements of global change affect herbivory in agroecosystems, a multifactorial experiment was conducted where soybeans were grown at two levels of carbon dioxide and temperature, including those predicted for 2050, under otherwise normal field conditions. japanese beetles (popillia japonica newman) were enclosed on foliage for 24 h, after which the beetle survivorship, total and per capita leaf consumption, and leaf protease inhibitor activity were measured. the direct e ...201323955947
elevated carbon dioxide increases salicylic acid in glycine max.concentrations of carbon dioxide (co(2)) are increasing in the atmosphere, affecting soybean (glycine max l.) phytohormone signaling and herbivore resistance. whether the impact of elevated co(2) on phytohormones and induced defenses is a generalized response within this species is an open question. we examined jasmonic acid (ja) and salicylic acid (sa) under ambient and elevated co(2) concentrations with and without japanese beetle (popillia japonica newman) damage and artificial damage across ...201223321090
role of cysteine proteinase inhibitors in preference of japanese beetles (popillia japonica) for soybean (glycine max) leaves of different ages and grown under elevated co2.elevated levels of co(2), equivalent to those projected to occur under global climate change scenarios, increase the susceptibility of soybean foliage to herbivores by down-regulating the expression of genes related to the defense hormones jasmonic acid and ethylene; these in turn decrease the gene expression and activity of cysteine proteinase inhibitors (cystpis), the principal antiherbivore defenses in foliage. to examine the effects of elevated co(2) on the preference of japanese beetle (jb; ...200919418071
longevity and fecundity of japanese beetle (popillia japonica) on foliage grown under elevated carbon dioxide.atmospheric levels of carbon dioxide (co(2)) have been increasing steadily over the last century. plants grown under elevated co(2) experience physiological changes that influence their suitability as food. previous studies have found increased insect herbivory on plants grown under elevated co(2). to determine effects of consuming foliage of soybean (glycine max) grown under elevated co(2) on adult survivorship and fecundity, japanese beetles (popillia japonica newman) were fed for the duration ...200818419934
transcriptional profiling reveals elevated co2 and elevated o3 alter resistance of soybean (glycine max) to japanese beetles (popillia japonica).the accumulation of co2 and o3 in the troposphere alters phytochemistry which in turn influences the interactions between plants and insects. using microarray analysis of field-grown soybean (glycine max), we found that the number of transcripts in the leaves affected by herbivory by japanese beetles (popillia japonica) was greater when plants were grown under elevated co2, elevated o3 and the combination of elevated co2 plus elevated o3 than when grown in ambient atmosphere. the effect of herbi ...200818194424
distribution and dynamics of japanese beetles along the indianapolis airport perimeter and the influence of land use on trap catch.as part of a program to minimize the accidental transportation of japanese beetles (popillia japonica) through cargo aircraft to areas where they are not established, a 4-yr trapping project was initiated to study the relative distribution and dynamics of the beetles along a trap line around the indianapolis international airport. land use influence on beetle abundance (trap catch) was assessed using a geographic information system. trap catch was consistently high in some locations and low in o ...200717445363
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