| drosophila melanogaster as an experimental host for study of multiplication and biology of the mycoplasma inducing the "lethargy of coleoptera". | the mulitplication of the mycoplasma responsible for the "lethargy of coleoptera" on a laboratory host, drosophila melanogaster, was obtained on the first passage. independant series of successive passages on d. melanogaster were performed without any apparent modifications of the properties of the microorganism. pathogenicity for its natural host melolontha melolontha was retained. the different forms of mycoplasma observed lead us to propose a probable cycle of development, composed of a succe ... | 1979 | 120120 |
| [comparative study of hemocyte capsule formation in locusta migratoria, melolontha melolontha and calliphora erythrocephala]. | | 1977 | 900777 |
| encapsulation of implanted foreign bodies by hemocytes in locusta migratoria and melolontha melolontha. | sterile fragments of cellophane, cat-gut, cotton, or beef muscle implanted into the body cavity of locusta migratoria and melolontha melolontha are immediately surrounded by granular hemocytes which form a multi-layered capsule around each foreign body. the cytoplasm of the granular hemocytes of the outer zone of the capsule in both species shows an accumulation of fibrous material (microtubules and microfilaments). none of the implanted material appeared to be degraded. | 1975 | 1097119 |
| [proceedings: behavior of bacillus popilliae in tissue culture of melolontha melolontha]. | | 1974 | 4444828 |
| [characteristics of infection of melolontha melolontha l. larvea by rickettsiella melolonthae krieg]. | | 1973 | 4799123 |
| cloning and sequencing of the spherulin gene, the occlusion body major polypeptide of the melolontha melolontha entomopoxvirus (mmepv). | in the late stage of infection, virions of the melolontha melolontha entomopoxvirus (mmepv) are occluded into cytoplasmic paracrysalline proteinaceous occlusion bodies designated spherules (a. amargier, c. vago, g. meynadier, 1964, mikroskopie 19, 309-315). we have cloned and sequenced a 4-kpb dna fragment of the mmepv genome encompassing the spherule major protein gene named spherulin. the spherulin gene contains an open reading frame able to code for a 942-amino-acid (aa) polypeptide (mw 109 k ... | 1994 | 8009856 |
| cloning and sequencing of the gene for an enhancing factor from pseudaletia separata entomopoxvirus. | an enhancing factor (ef) has been shown to increase the susceptibility of the armyworm, pseudaletia separata, to this nuclear polyhedrosis virus. using amino acid microsequencing data obtained from the purified ef protein, we cloned and sequenced the gene for ef. the deduced amino acid sequence exhibited a significant similarity to major spindle proteins, fusolins, whose function is unknown, from heliothis armigera, choristoneura biennis and melolontha melolontha entomopoxviruses. | 1996 | 8921880 |
| cloning, sequencing and transcriptional analysis of the choristoneura fumiferana entomopoxvirus spheroidin gene. | the choristoneura fumiferana entomopoxvirus (cfepv) spheroidin gene was identified and localized on three xbai restriction fragments (total size 4.73 kb). the fragments were cloned and sequenced. the spheroidin gene had an open reading frame of 2997 nucleotides encoding a putative protein with a predicted size of 115 kda. sequence analysis indicated that the putative protein contained 14 potential n-glycosylation sites (asn-x-thr; asn-x-ser), that are probably not used since the protein migrates ... | 1997 | 9085546 |
| cloning and analysis of the first cry gene from bacillus popilliae. | an 80-kda parasporal crystal protein was detected in protein extracts of sporangia of bacillus popilliae isolated from a diseased larva of the common cockchafer (melolontha melolontha l.). amino acid analysis of tryptic peptides revealed significant homology to the cry2aa endotoxins of bacillus thuringiensis. the gene crybp1 (cry18aa1), which codes for the parasporal crystal protein, was found in a putative cry operon on the bacterial chromosome, which contains at least one further (smaller) ope ... | 1997 | 9209052 |
| spiroplasmas: infectious agents of plants, arthropods and vertebrates. | the spiroplasmas are mollicutes characterized by motility and helical morphology. they were discovered through studies on corn stunt and citrus stubborn diseases. the stubborn agent was the first mollicute of plant origin to be obtained in culture and the first cultured mollicute to possess a helical morphology. the citrus pathogen has been known as spiroplasma citri since 1973. the corn stunt agent was cultured in 1975 and fully characterized as spiroplasma kunkelii by 1986. the third and only ... | 1997 | 9286068 |
| transient, nonlethal expression of genes in vertebrate cells by recombinant entomopoxviruses. | the group b entomopoxvirus (epv) from amsacta moorei (amepv) productively infects only insect cells. a series of amepv-lacz recombinants was constructed in which the lacz gene was regulated by either late (the amepv spheroidin or the cowpox virus a-type inclusion [ati]) or early (the amepv esp [early strong promoter; derived from a 42-kda amepv protein] or the melolontha melolontha epv fusolin, fus) virus promoters. when the amepv recombinants were used to infect vertebrate cells, beta-galactosi ... | 1997 | 9371619 |
| characterization of the proteolytic enzymes in the midgut of the european cockchafer, melolontha melolontha (coleoptera: scarabaeidae). | in previous studies we showed that the resistance of the european cockchafer, melolontha melolontha, towards the scarab specific cry8c toxin of bacillus thuringiensis japonensis strain buibui is due to the complexity of proteinases in the midgut of the pest insect. in this study these proteinases were identified and characterized using a combination of synthetic substrates and specific inhibitors in zymograms, activity blots, and photometric/fluorometric assays. in the midgut juice three trypsin ... | 2002 | 12044497 |
| [so-called milky disease of larvae of melolontha melolontha l. (coleptera scarabeidae)]. | | 1955 | 13317264 |
| [the virulence of bacillus popilliae dutky for the larvae of the common cockchafer (melolontha melolontha l]. | | 1957 | 13573740 |
| accurate determination of oosporein in fungal culture broth by differential pulse polarography. | a simple and accurate differential pulse polarographic method has been developed for the determination of oosporein in the culture broth of the fungus beauveria brongniartii. this hydroxybenzoquinone derivative is the only major secondary metabolite secreted by this entomopathogenic fungus, which is used as biological pest control agent (bca) against melolontha melolontha larvae. it can be found in the host organism as well as in the formulated product. the polarographic behavior of oosporein wa ... | 2004 | 15030190 |
| structure and topology of microbial communities in the major gut compartments of melolontha melolontha larvae (coleoptera: scarabaeidae). | physicochemical gut conditions and the composition and topology of the intestinal microbiota in the major gut compartments of the root-feeding larva of the european cockchafer (melolontha melolontha) were studied. axial and radial profiles of ph, o2, h2, and redox potential were measured with microsensors. terminal restriction fragment length polymorphism (t-rflp) analysis of bacterial 16s rrna genes in midgut samples of individual larvae revealed a simple but variable and probably nonspecific c ... | 2005 | 16085849 |
| cry3aa11: a new cry3aa delta-endotoxin from a local isolate of bacillus thuringiensis. | a local isolate of bacillus thuringiensis mm2 had insecticidal activity against the larvae of melolontha melolontha, agelastica alni, leptinotarsa decemlineata and amphimallon solstitiale and produced a 65 kda protein. sds-page profile of b. thuringiensis mm2 was compared with those of 29 different cry3aa producers which verified cry3aa biosynthesis by the isolate. the cry3aa gene of mm2 was cloned, sequenced and the deduced amino acid sequence was compared with the cry3aa sequences of ten diffe ... | 2005 | 16132862 |
| investigations on bacteria as a potential biological control agent of summer chafer, amphimallon solstitiale l. (coleoptera: scarabaeidae). | studying the bacteria of hazardous insects allows the opportunity to find potentially better biological control agents. therefore, in this study, bacteria from summer chafer (amphimallon solstitiale l., coleoptera: scarabaeidae) we isolated and identified the insecticidal effects of bacteria isolated from a. solstitiale and melolontha melolontha l. (common cockchafer, coleoptera: scarabaeidae) and the mixtures of these bacterial isolates were investigated on a. solstitiale larvae. crystals from ... | 2005 | 16273040 |
| persistence and efficacy of beauveria brongniartii strains applied as biocontrol agents against melolontha melolontha in the valley of aosta (northwest italy). | to monitor and select genetically characterized strains of beauveria brongniartii to be used as microbiological control agents against melolontha melolontha in different climatic conditions of the valley of aosta (northwest italy). | 2006 | 16630007 |
| [entomopoxviruses and biological control.] | poxviridae are divided into two subfamilies: the chordopoxvirinae (poxviruses of vertebrates) and the entomopoxvirinae (insect poxviruses). entomopoxviruses (epvs) are large (300-400 nm) oval shaped viruses. the genome of epvs is large, with covalent ends and is a linear double-stranded dna (200-240 kbp) molecule. the entomopoxvirinae comprises three genera based on host insect and virion morphology. genus a viruses infect coleopterans, genus b viruses infect lepidopterans and orthopterans, and ... | 2005 | 17124689 |
| characterization of a highly pathogenic bacillus thuringiensis strain isolated from common cockchafer, melolontha melolontha. | a bacterial isolate (mm2) of melolontha melolontha was identified and characterized. based on various morphological, physiological, biochemical and molecular characteristics, it was identified as bacillus thuringiensis subsp. tenebrionis. this isolate was compared to the reference strains by electron microscopy, sds-page analysis, plasmid pattern, cry gene content and insecticidal activity. cells of the isolate harbored flat square inclusions containing a protein component of approximately equal ... | 2007 | 17575913 |
| 16s rrna-, groel- and mucz-based assessment of the taxonomic position of 'rickettsiella melolonthae' and its implications for the organization of the genus rickettsiella. | 'rickettsiella melolonthae' is an intracellularly multiplying bacterial pathogen of european cockchafers, melolontha melolontha (linnaeus, 1758) and melolontha hippocastani (fabricius, 1801) (coleoptera: scarabaeidae). we report the first determination of nucleotide sequences from this organism, i.e. the 16s rrna encoding rrs gene, the chaperonin encoding groel gene and the mucz gene encoding the orthologue of a capsule synthesis-inducing factor of coxiella burnetii. within the genus rickettsiel ... | 2008 | 18398164 |
| carbon utilization pattern as a potential quality control criterion for virulence of beauveria brongniartii. | the registered entomopathogenic fungus beauveria brongniartii (bipesco 2) was tested for its virulence after one, five and 10 times sub-culturing on four types of selective synthetic nutrient media. bioassays with third instar melolontha melolontha larvae showed that sub-culturing negatively affects the virulence of the fungus after 10 transfers. with the biolog sf-p2 and biolog sf-n2 microtiter plate systems the sub-cultivated b. brongniartii conidia were monitored for any change in the carbon ... | 2010 | 20123102 |
| assessment of gut bacteria for a paratransgenic approach to control dermolepida albohirtum larvae. | bacteria from the hindguts of dermolepida albohirtum larvae were assessed for their potential to be used in paratransgenic strategies that target scarab pests of sugarcane. bacteria isolated in pure culture from the hindguts of d. albohirtum larvae were from the proteobacteria, firmicutes, and actinobacteria phyla and matched closely with taxa from intestinal and rhizosphere environments. however, these isolates were not the most common gut-associated bacteria identified in denaturing gradient g ... | 2008 | 18456847 |
| development of a method for detection and quantification of b. brongniartii and b. bassiana in soil. | a culture independent method based on qpcr was developed for the detection and quantification of two fungal inoculants in soil. the aim was to adapt a genotyping approach based on ssr (simple sequence repeat) marker to a discriminating tracing of two different species of bioinoculants in soil, after their in-field release. two entomopathogenic fungi, beauveria bassiana and b. brongniartii, were traced and quantified in soil samples obtained from field trials. these two fungal species were used a ... | 2016 | 26975931 |
| complete nucleotide sequence of spheroidin gene of anomala cuprea entomopoxvirus. | the complete nucleotide sequence of the spheroidin gene of anomala cuprea entomopoxvirus (acepv) was determined. the sequence was compared with those of spheroidin genes of epvs such as melolontha melolontha epv (mmepv, genus a), choristoneura fumiferana epv (cfepv, genus b) and amsacta moorei epv (amepv, genus b). the gene harbored an open reading frame (orf) of 2826 nt, with the same size as that of mmepv belonging to the same genus, capable of coding for a polypeptide of 109.0 kda. the predic ... | 1998 | 9712512 |
| complete nucleotide sequence of the fusolin gene of an entomopoxvirus in the cupreous chafer, anomala cuprea hope (coleoptera: scarabaeidae). | the fusolin gene of anomala cuprea entomopoxvirus (acepv) was cloned and sequenced. the sequence was compared with that of fusolin genes of epvs from other insects such as melolontha melolontha (mmepv), choristoneura biennis (cbepv) and heliothis armigera (haepv) previously reported. the gene harbored a single open reading frame (orf) of 1119 nt capable of coding for a protein of 43.3 kda. the microsequencing of the protein showed the cleavage of a signal peptide of 16 amino acid (aa) residues. ... | 1997 | 9474783 |
| alcoholism in cockchafers: orientation of male melolontha melolontha towards green leaf alcohols. | chemical orientation of the european cockchafer, melolontha melolontha l., a serious pest in agriculture and horticulture, was investigated by field tests and electrophysiological experiments using plant volatiles. in total, 16 typical plant volatiles were shown to elicit electrophysiological responses in male cockchafers. funnel trap field bioassays revealed that green leaf alcohols (i.e. (z)-3-hexen-1-ol, (e)-2-hexen-1-ol and 1-hexanol) attracted males, whereas the corresponding aldehydes and ... | 2002 | 12146792 |
| entomopathogenic nematodes in the european biocontrol market. | in europe total revenues in the biocontrol market have reached approximately 200 million euros. the sector with the highest turn-over is the market for beneficial invertebrates with a 55% share, followed by microbial agents with approximately 25%. annual growth rates of up to 20% have been estimated. besides microbial plant protection products that are currently in the process of re-registration, several microbial products have been registered or are in the process of registration, following the ... | 2003 | 15149088 |
| a case study on toxicological aspects of the pest and disease control in the production of the high-quality raspberry (rubus idaeus l.). | the field studies on the residue levels of the fungicides and insecticides used in commercial raspberry (rubus idaeus l.) plantation have been performed. starting on the first day of harvesting (on june 19), 20 laboratory samples of fruit, 10 laboratory samples of leaves and 4 samples of soil were analyzed and the residue levels were compared to the maximum residue limits (mrl) and acceptable daily intakes (adi). all analyses were carried out using extraction method and gas chromatography techni ... | 2015 | 25421623 |