Publications

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nitrogenous excretory products of adults of sitophilus oryzae and sitophilus granarius. 19761248209
ige-mediated inhalant allergy in inhabitants of a building infested by the rice weevil (sitophilus oryzae).the rice weevil (sitophilus oryzae) is known as a stored product pest. occupants living in a factory used previously as a granary and known to be contaminated by the rice weevil developed rhinitis and asthma. to study the role of the potential insect allergens, extracts were prepared from whole body and a grain dust of the rice and grain weevil (sitophilus granarius). the extracts were coupled to cyanogen bromide-activated paper disks. skin prick tests (spt) were performed using common inhalant ...19921471781
t-independent polyclonal activation of b cells in vitro by immunoglobulin binding substance (ibs) from the granary weevil.experiments are described for t-independent polyclonal activation of b cells in vitro by the immunoglobulin binding substance (ibs) from the granary weevil. the affinity-chromatographically purified ibs was used. ibs is a heat-, alkali- and acid-stable glucopeptide which is characterized by non-specific immunoglobulin binding to the fab fragment. the purified ibs consists of three polymer homologues whose molecular weights are 12-14,000, 25-30,000 and more than 150,000 da. ibs did not stimulate ...19921734493
extramitochondrial release of hydrogen peroxide from insect and mouse liver mitochondria using the respiratory inhibitors phosphine, myxothiazol, and antimycin and spectral analysis of inhibited cytochromes.the fumigant insecticide phosphine (ph3) is known to inhibit cytochrome c oxidase in vitro. inhibition of the respiratory chain at this site has been shown to stimulate the generation of superoxide radicals (o2-), which dismutate to form hydrogen peroxide (h2o2). this study was performed in order to investigate the production of h2o2 by mitochondria isolated from granary weevil (sitophilus granarius) and mouse liver on exposure to ph3. other respiratory inhibitors, antimycin, myxothiazol, and ro ...19902321971
property of immunoglobulin binding substance from the granary weevil. 19854038089
cytochemistry, morphogenesis, and tentative identification of myecetomal microorganisms of sitophilus granarius l. (coleoptera). 19704107100
differential net food utilization by larvae of sitophilus oryzae and sitophilus granarius. 19744420184
ultrastructure of the lateral neurosecretory cells during reproductive development of sitophilus granarius (l.) (insecta: coleoptera). 19724656291
susceptibility of two strains of sitophilus granarius to 1,2-dibromoethane. 1. effect of weight-dependent respiration and fumigant uptake on strain susceptibility. 19725008987
dichlorvos and gardona as protectants for stored wheat against granary weevil infestations in laboratory studies. 19725085804
sexual dimorphism in sitophilus granarius (l.) as viewed in the scanning electron microscope. 19715094124
aspects of histophysiology of the spermathecal gland of sitophilus granarius (l.) (coleoptera). 19715101971
the influence of oxygen on the metabolism of sitophilus granarius (l.) during cyanide poisoning. 19655827536
some effects of desiccation on locomotion of the grain beetle, sitophilus granarius (l.) (coleoptera: curculionidae). 19665919295
millworkers' asthma: allergic responses to the grain weevil (sitophilus granarius). 19665929689
locomotory behaviour of pyrethrum-resistant and susceptible strains of grain weevil, sitophilus granarius (l.) (coleoptera, curculionidae). 19665956582
[extrinsic allergic alveolitis: comparison of 2 methods (ouchterlony gel precipitation and elisa) for antibody detection in routine diagnosis].sera from 58 patients with suspected extrinsic allergic alveolitis (eaa) were tested in parallel by the double diffusion test and the "enzyme-linked immunosorbent assay (elisa)" using 10 different antigen solutions (extracts from thermophilic actinomycetes, various mycetes, pigeon serum, pigeon droppings and the wheat weevil sitophilus granarius). the elisa technique was more sensitive than the double diffusion test in detecting antibodies to the panel of antigens used. however, by using several ...19836422645
a unique substance from the granary weevil: nonspecific immunoglobulin binding substance.we have studied the precipitation test as a diagnostic procedure for hypersensitivity pneumonitis or associated allergic states using sitophilus granarius (sg) antigen. the incidence of positive reaction in the precipitation test against sg is extremely high in children and adults, not only asthmatics, but also in normal, healthy subjects. in experiments with radioimmunoassay using sg paper discs and 125i-labelled igg fragments, sg was more strongly bound to the fab than to the fc fragment. howe ...19836642706
stored wheat insect infestation related to uric acid as determined by liquid chromatography.liquid chromatography is used to measure the uric acid content of wheat internally infested by various growth stages of granary weevil (sitophilus granarius), rice weevil (sitophilus oryzae), and lesser grain borer (ryzopertha dominica). good correlation exists between numbers of insects and uric acid content of grain infested by a given stage of an internally infesting stored product insect, with correlation coefficients ranging from 0.970 to 0.998. uric acid content of infested grain increases ...19846746490
hypersensitivity pneumonitis: occupational exposure to sitophilus granarius and thermoactinomyces vulgaris. 19827162213
[some observations on farmer's lung (author's transl)].sera from 72 patients--29 females, 43 males--with symptoms suggestive of farmer's lung were tested by the ouchterlony double diffusion technique using 10 different antigen solutions (extracts from mycetes and thermophilic actinomycetes, pigeon serum, extracts from pigeon droppings and sitophilus granarius). in 23 cases precipitating antibodies were observed and micropolyspora faeni was the most common antigen responsible for positive reactions. in 17 seropositive patients the diagnosis of exogen ...19817342469
identification of important allergenic proteins in extracts of the granary weevil (sitophilus granarius).this paper describes the identification of important allergens from the granary weevil (sitophilus granarius) (sg). sera from danish bakers whose skin prick tests were positive to extracts of sg were screened for ige against sg extracts. we found that 54% (n = 66) had elevated levels of ige (rast classes 1-3, by luminescent immunoassay) against whole-body extracts of sg. the specificity of patient ige was investigated in an inhibition-dot immunoblotting assay. ige binding was inhibited in all se ...19957573834
in vitro diagnosis of farmers' ige-mediated allergy by phadiatop and three new multiallergen rast analyses.identification of persons with atopic allergy in a population is difficult if the pattern of sensitizing allergens is unique. three new multiallergen tests, professional allergy mixes (pax) 1-3 (pharmacia diagnostics, uppsala, sweden), have recently been introduced to screen ige-mediated allergy among farmers. pax 1 contains animal danders and feathers, pax 2 mites and insects, and pax 3 pollens and molds. the value of these new tests for the determination of ige-mediated allergy among farmers w ...19938368461
four intracellular genomes direct weevil biology: nuclear, mitochondrial, principal endosymbiont, and wolbachia.cell physiology in the weevil sitophilus oryzae is coordinated by three integrated genomes: nuclear, mitochondrial, and the "s. oryzae principal endosymbiont" (sope). sope, a cytoplasmic bacterium (2 x 10(3) bacteria per specialized bacteriocyte cell and 3 x 10(6) bacteria per weevil) that belongs to the proteobacteria gamma3-subgroup, is present in all weevils studied. we discovered a fourth prokaryotic genome in somatic and germ tissues of 57% of weevil strains of three species, s. oryzae, sit ...199910359795
control of insects and mites in grain using a high temperature/short time (htst) technique.wheat infested with grain mites (acari) and sitophilus granarius, and maize infested with prostephanus truncatus, were exposed to hot air in a cimbria htst microline toaster((r)). inlet temperatures of the hot air were in the range of 150-750 degrees c decreasing to outlet temperatures in the range of 100-300 degrees c during the exposure period. a rotating drum, connected to a natural-gas burner was fed with grain which was in constant movement along the drum and thereby mixed thoroughly during ...200010758269
lactones. 9. synthesis of terpenoid lactones-active insect antifeedants.starting from (+)- and (-)-perillyl alcohols, via claisen rearrangement and iodolactonization, four enantiomeric pairs of gamma-lactones were obtained. the structures of compounds were established by both spectroscopic and crystallographic methods. the lactones were tested for antifeeding activity toward grain storage pests-the granary weevil beetle (sitophilus granarius l.), the khapra beetle (trogoderma granarium ev.), and the confused flour beetle (tribolium confusum duv.). the results of the ...200011052764
action of amorphous diatomaceous earth against different stages of the stored product pests tribolium confusum, tenebrio molitor, sitophilus granarius and plodia interpunctella.environmental and human health problems associated with the use of synthetic pesticides have prompted the demand for non-polluting, biologically specific insecticides. the current study tested the use and action of diatomaceous earth against several stored product pests. fossil shield(r) applied to wooden plates was lethal to adult tenebrio molitor and tribolium confusum, but larvae of the mealworm were unaffected. beetles died within 14 days exposure in the absence of food to a dose of 2 and 4 ...200111124378
the effect of gamma and microwave irradiation of wheat grain on development parameters of some stored grain pests.wheat grain was treated with different doses of gamma irradiation or different times of microwaves. gamma rays (0.05-10 kgy) caused statistically significant differences in soluble protein contents and kernel hardness. microwave treatment (15-180 s) resulted in some statistically significant changes in moisture and soluble protein contents. mortality, intensity of feeding, fertility of adults, progeny number and development time of larvae of the granary weevil (sitophilus granarius l.) on treate ...200011190835
lactones: part 11. feeding-deterrent activity of some bi- and tricyclic terpenoid lactones.the feeding-deterrent activities of thirteen synthetic terpenoid lactones, including isomeric bicyclic gamma-spirolactones with the limonene system and tricyclic gamma-lactones with the pinane system, were determined towards three storage pest insects: the adults of sitophilus granarius, the adults and larvae of tribolium confusum and the larvae of trogoderma granarium. the configuration of chiral centres, as well as the presence of additional functional groups (double bond, iodine and hydroxy g ...200111561401
repeated losses of ttagg telomere repeats in evolution of beetles (coleoptera).we studied the occurrence of (ttagg)n telomere repeats in 12 species of beetles, representing main lineages of the coleoptera phylogenetic tree, by southern hybridization and fluorescence in situ hybridization (fish). in contrast to other insect orders, beetles were heterogeneous with respect to the occurrence of ttagg repeats. in addition, the presence or absence of (ttagg)n motif was irrespective of phylogenetic relationships. in the suborder polyphaga, six species displayed positive hybridiza ...200212403172
toxicity of cyanogen to insects of stored grain.range-finding studies on the toxicity of cyanogen to all stages of five species of stored product coleoptera are reported. the species were rhyzopertha dominica (f), sitophilus granarius (l), sitophilus oryzae (l), tribolium castaneum (herbst), tribolium confusum jacquelin du val and ephestia cautella (walker). exposures for 24 h to cyanogen at 1.3 mg litre(-1) controlled all external stages. control of internal stages of sitophilus species was achieved by a 5-day exposure to initial concentrati ...200312639054
specific foraging kairomones used by a generalist parasitoid.in general, it is assumed that generalist natural enemies do not innately use specific cues for the location of their host or prey species. this hypothesis was tested using naïve females of the generalist parasitoid lariophagus distinguendus förster and two of its hosts, larvae of the lesser grain borer rhyzopertha dominica (f.) and of the granary weevil sitophilus granarius l., feeding in wheat grains. in a four-chamber olfactometer, female parasitoids were attracted to volatiles emanating from ...200312647858
characterization of a high-affinity binding site for the pea albumin 1b entomotoxin in the weevil sitophilus.the toxicity of the pea albumin 1b (pa1b), a 37 amino-acid peptide extracted from pea seeds, for cereal weevils (sitophilus oryzae, sitophilus granarius and sitophilus zeamais) was recently discovered. the mechanism of action of this new entomotoxin is still unknown and potentially involves a target protein in the insect tissues. this work describes the characterization of a high-affinity binding site for pa1b in a microsomal fraction of sitophilus spp. extracts. purified pa1b was labeled to a h ...200312755698
the effect of cold acclimation and deacclimation on cold tolerance, trehalose and free amino acid levels in sitophilus granarius and cryptolestes ferrugineus (coleoptera).canadian and french laboratory strains of sitophilus granarius (l.) and cryptolestes ferrugineus (stephens) were cold acclimated by placing adults at 15, 10 and 5 degrees c successively for 2wk at each temperature before deacclimating them for 1wk at 30 degrees c. unacclimated s. granarius had an lt(50) (lethal time for 50% of the population) of 12days at 0 degrees c compared with 40days after the full cold acclimation. at -10 degrees c, unacclimated c. ferrugineus had an lt(50) of 1.4days compa ...199812770432
apparent effect on the mycetomal microorganisms of repeated exposure of the host insect, sitophilus granarius (l.) (coleoptera), to methyl bromide fumigation. 196113727084
the action of fumigants on insects. iii. the fate of hydrogen cyanide in sitophilus granarius (l.). 196113870922
[the protection of foods against ravaging insects by ionizing radiations, with a view to obtaining sterility of the adult insects. special study of the reaction of the gonads of "sitophilus granarius"]. 196314104870
pa1b, an insecticidal protein extracted from pea seeds (pisum sativum): 1h-2-d nmr study and molecular modeling.pa1b (pea albumin 1, subunit b) is a 37-amino acid cysteine-rich plant defense protein isolated from pea seeds (pisum sativum). it induces short-term mortality in several pests, among which the cereal weevils sitophilus sp. (sitophilus oryzae, sitophilus granarius, and sitophilus zeamais) that are a major nuisance for stored cereals, all over the world. as such, pa1b is the first genuine protein phytotoxin specifically toxic to insects, which makes it a promising tool for seed weevil damage cont ...200314556622
insecticidal activity of the enantiomers of fipronil.the two enantiomers of the insecticide fipronil were made by preparative hplc. the insecticidal activities of the racemic mixture and the two enantiomers against selected agricultural or household pests (cotton stainer, dysdercus cingulatus f; grain weevil, sitophilus granarius l and house fly, musca domestica l) were determined. there was no significant difference in acute or residual activity between the racemic mixture and the enantiomers of fipronil, indicating that there is no preferred chi ...200314667049
synthesis and insect antifeedant activity of precocene derivatives with lactone moiety.starting from precocenes i and ii, four of their derivatives with a lactone moiety were obtained. the compounds have been assessed as antifeedants against several diverse insect species including the storage pests the confused flour beetle (tribolium confusum duv., larvae and adults), the granary weevil beetle (sitophilus granarius l., adults), and the khapra beetle (trogoderma granarium ev., larvae) and against the herbivorous pest insects colorado potato beetle (leptinotarsa decemlineata say, ...200516028972
host habitat assessment by a parasitoid using fungal volatiles.the preference-performance hypothesis predicts that oviposition preference of insects should correlate with host suitability for offspring development. therefore, insect females have to be able to assess not only the quality of a given host but also the environmental conditions of the respective host habitat. chemical cues are a major source of information used by insects for this purpose. primary infestation of stored grain by stored product pests often favors the intense growth of mold. this c ...200717284315
biocontrol potential of lariophagus distinguendus (hymenoptera: pteromalidae) against sitophilus granarius (coleoptera: curculionidae) at low temperatures: reproduction and parasitoid-induced mortality.lariophagus distinguendus forster (hymenoptera: pteromalidae) has been suggested as a biological control agent against the granary weevil, sitophilus granarius (l.), in grain stores. information on the effect of low temperatures prevailing in grain stores is necessary to be able to predict the potential of this parasitoid against s. granarius in temperate regions, where grain is cooled with ambient air to achieve safe storage conditions. the influence of constant temperatures of 16, 18, and 20 d ...200717598568
behavioral responses of adult sitophilus granarius to individual cereal volatiles.the antennae of sitophilus granarius (l.) (coleoptera: curculionidae) adults detect a wide variety of compounds in the odor blend of various cereal grains (germinara et al., tec. molit., 53:27-34, 2002). in the present study, we looked at the behavioral responses of the granary weevil to 20 of these individual volatiles (aliphatic alcohols, aldehydes, ketones, and aromatics) in a two-choice pitfall olfactometer, using the aggregation pheromone and propionic acid as the attractant and repellent c ...200818340486
antifungal, phytotoxic and insecticidal properties of essential oil isolated from turkish origanum acutidens and its three components, carvacrol, thymol and p-cymene.the chemical composition of essential oil isolated by hydrodistillation from the aerial parts of origanum acutidens was analyzed by gc-ms. carvacrol (87.0%), p-cymene (2.0%), linalool acetate (1.7%), borneol (1.6%) and beta-caryophyllene (1.3%) were found to be as main constituents. antifungal, phytotoxic and insecticidal activities of the oil and its aromatic monoterpene constituents, carvacrol, p-cymene and thymol were also determined. the antifungal assays showed that o. acutidens oil, carvac ...200818513954
molecular techniques for the detection of granary weevil (sitophilus granarius l.) in wheat and flour.the granary weevil (sitophilus granarius l.) is a stored grain pest that causes major economic losses. it reduces the quantity and quality of the grain by its feeding and excretion. sequences of s. granarius mitochondrial cytochrome oxidase subunits genes mtcoi and mtcoii were analysed and compared with mtcoi/ii sequences available in genbank. the analysed genes displayed a high level of homology between corresponding subunits. attempts were undertaken to develop detection methods for contaminat ...200818608514
scanning electron microscopy of the spermatheca in sitophilus granarius (l.).with scanning electron microscopy the spermathecal organ of sitophilus granarius (l.) has been examined. the only morphological attachment between this organ and other tissues is to the bursa copulatrix by the sperm duct and to the central nervous system by two nerve fibers. the nerves innervate the muscles connecting the two arms of the u-shaped spermatheca. glandular cells are grouped together and are connected to the spermathecal lumen by a short collecting duct. vascular sinuses opening from ...197118631570
interaction of radio-frequency, high-strength electric fields with harmful insects.the objective of the research reported here is to investigate the influence of radio-frequency electric fields of high strength on insect mortality. the experiments were accomplished at the frequencies 47.5, 900 and 2,450 mhz for the pulse modulated radiation treatment and the continuous wave rf radiation. two types of systems, which are the coaxial irradiation chamber and the irradiation chamber with plane capacitor are presented in this work. the experiments in the coaxial type radiation chamb ...200921384727
ethyl formate plus methyl isothiocyanate-a potential liquid fumigant for stored grains.background: methyl bromide is being phased out for use on stored commodities, as it is listed as an ozone-depleting substance, and phosphine is the fumigant widely used on grains. however, phosphine resistance occurs worldwide, and phosphine fumigation requires a long exposure period and temperatures of > 15 °c. there is an urgent requirement for the development of a fumigant that kills insects quickly and for phosphine resistance management. this paper reports on a new fumigant formulation of 9 ...201121780282
bioactivity of short-chain aliphatic ketones against adults of the granary weevil, sitophilus granarius (l.).background: the granary weevil, sitophilus granarius (l.), is one of the most damaging pests of stored grains, causing severe quantitative and qualitative losses. sustainable control means, alternative to the commonly used fumigants and broad-spectrum contact insecticides, are urgently needed owing to legislative limits, the development of resistant insect strains and increasing consumer demand for safe food. short-chain aliphatic ketones, known to be emitted by cereal grains and previously iden ...201122081494
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