Publications

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oxygen uptake in male and female boll weevils, anthonomus grandis boheman (coleoptera: curculionidae) of the ebony strain fed a laboratory diet. 19764277
evidence of a chemotactic substance in the spermathecal gland of the female boll weevil (coleoptera: curculionidae). 1978564566
genotypes of esterase ii determined from frass of anthonomus grandis boh. (coleoptera, curculionidae).esterase ii was separated, using gel electrophoresis, from the frass of the cotton boll weevil, allowing genotyping of the individual weevil. the method and its application are discussed.1977603619
chemosterilant and insecticidal activity of mixed aflatoxins against anthonomus grandis (coleoptera). 1978681770
role of phermones and kairmones for insect suppression systems and their possible health and environmental impacts.insects produce pheromones as a chemical communication system to facilitate reproduction. these highly active chemical attractants have been synthesized for some of the most important insect pests, including the boll weevil, gypsy moth, codling moth, tobacco budworm, european corn borer, and several bark beetles. while none of the synthetic sex attractants have yet been developed for use in insect control, they offer opportunities for the future both as control agents and to greatly improved ins ...1976789061
succinate-cytochrome c reductase activity and lipids in diapause and non-diapause anthonomus grandis from different latitudes. 1977853236
esterases of laboratory-reared and field-collected cotton boll weevils, anthonomus grandis boh.: polymorphism of adult esterases and formal genetics of esterase ii.the esterases of the cotton boll weevil were separated by polyacrylamide gel electrophoresis into four major regions. these were named est i-iv in order of migration from anode to origin. polymorphism was observed in all regions. the est ii region was shown to consist of no more than two bands (fast and slow). the inheritance of the fast and slow bands of est ii was demonstrated to be controlled by codominant autosomal alleles. analysis of the gene frequency of the est ii region showed that one ...1977869895
identification of the oxidative decomposition products of the boll weevil pheromone, grandlure, and the determination of the fate of grandlure in soil and water. 1976943430
a facile synthesis of the cyclohexyl constituents of the boll weevil sex pheromone. 1976943480
studies on terpenes. 4. synthesis of optically active grandisol, the boll weevil pheromone. 1976945309
biochemical changes in the boll weevil induced by rose bengal in the absence of light. 1976954138
sterilization of the boll weevil: vacuum fumigation with hempa combined with feeding busulfan-treated diet. 19751141484
a biogenetic-type synthesis of the cyclohexyl constituents of the boll weevil pheromone. 19751170288
transfection of cultured cells of the cotton boll weevil, anthonomus grandis, with a heat-shock-promoter-chloramphenicol-acetyltransferase construct.expression of heat shock proteins (hsp) in the brl-ag-3c cell line from the cotton boll weevil was examined. it was determined that the maximal expression of endogenous hsp occurred at 41 degrees c. various transfection methods were then compared using this cell line in conjunction with a transiently expressed bacterial gene marker (chloramphenicol acetyltransferase) which was under the control of the drosophila hsp 70 gene promoter. the cationic lipid preparation lipofectin was found to be very ...19921343780
why is there sequence similarity between insect yolk proteins and vertebrate lipases?the major proteins stored in the yolk of developing oocytes are thought to provide a nutritional store for utilization during embryogenesis. they seem to fall into two major families of proteins. the first are called vitellogenins and are found in frog, chicken, nematode, fish, and some insects such as the boll weevil. the other group are called yolk proteins and are found in dipteran insects such as fruitfly, housefly, fleshfly, and blue-bottles. both groups are the major proteins found in the ...19921512506
replication of autographa californica baculovirus (acmnpv) in a coleopteran cell line.a coleopteran cell line (age) derived from the cotton boll weevil anthonomus grandis supported replication of autographa californica multiple nuclear polyhedrosis virus (acmnpv). the titer of extracellular virus (ecv) and the number of occlusion bodies (ob) produced in age cells were approximately equal to those produced by a trichoplusia ni cell line (tn-cl1), and the ob produced by both cell lines were equally infectious for t. ni larvae. the identity of the age cell line was established by ch ...19921522049
the boll weevil vitellogenin gene: nucleotide sequence, structure, and evolutionary relationship to nematode and vertebrate vitellogenin genes.boll weevil (anthonomus grandis) eggs contain two yolk proteins, yp47 and yp160. using anti-yp160 antiserum as probe, a partial-length complementary dna (cdna) was isolated from a lambda gt11 adult female cdna library. a second partial-length cdna was isolated from a lambda gt10 adult female cdna library by differential screening with male vs. female cdnas. northern blot analysis showed that each cloned cdna hybridized to a 6-kb female-specific transcript. these cdnas were used to probe a genomi ...19921593641
boll weevil testis-specific cdna. 19902362819
distribution and probable physiological role of esterases in reproductive, digestive, and fat-body tissues of the adult cotton boll weevil, anthonomus grandis boh.polyacrylamide gel electrophoresis was used to examine gut, malpighian tube, fat-body, testes, and ovarioles tissues of the adult cotton boll weevil, anthonomus grandis boh. esterases for which the inheritance has been reported previously by terranova using whole-body homogenates were detected in dissected tissues and the probable physiological function of each allozyme is suggested. est-1 occurs most frequently in ovarioles and female fat bodies. est-2 is most often found in fat bodies and may ...19863741371
an assessment of the life time biosynthesis potential of the male boll weevil. 19744411957
biosynthesis of grandlure, the pheromone of the boll weevil, anthonomus grandis, from acetate, mevalonate, and glucose. 19744412207
free amino acid levels in the boll weevil (coleoptera: curculionidae): the effect of five different diets. 19744434735
effects of aldicarb applied side-dress to cotton on some arthropods in the monroe county, mississippi, boll weevil diapause-control area in 1969. 19734690259
chemosterilization of the female boll weevil. 19734690295
constituents of the boll weevil, anthonomus grandis boheman. 8. lipids and fatty acids in the subcellular particles of pharate pupae. 19734719989
analysis of the antimicrobial agents, potassium sorbate and methyl-p-hydroxybenzoate, in boll weevil diets. 19744833468
constituents of the boll weevil. vi. effect of free fatty acid content larval development. 19725085786
field tests for control of heliothis spp. with a nuclear polyhedrosis virus included in a boll weevil bait. 19715092854
the residual fate of n,n,n',n'-tetramethyl-p-piperidinophosphonic diamide after injection, tarsal contact, and topical application to the boll weevil. 19695359170
insect sterilant experiments with apholate and five bifunctional aziridine chemicals in outdoor cages against the boll weevil. 19695377048
synthetic studies on insect hormones. ix. stereoselective total synthesis of a racemic boll weevil pheromone. 19705460980
physicochemical characteristics of glycogen from anthonomus grandis boheman. 19685645507
cholinesterase in boll weevils, anthonomus grandis boheman. i. distribution and some properties of the crude enzyme. 19685653718
the free amino acids in the haemolymph of the maturing adult boll weevil, anthonomus grandis boheman. 19685657191
boll weevil found in pre-columbian cotton from mexico. 19685684498
axenic rearing of the boll weevil on defined diets: amino acid, carbohydrate, and mineral requirements. 19655827528
synthesis and metabolic conversion of fatty acids by the larval boll weevil. 19655866060
storage and excretion of steroids in the adult boll weevil. 19655893695
the lipid-class spectrum and fatty-acid content of the boll weevil egg. 19665967421
lipid biosynthesis in the boll weevil (anthonomus grandis boheman) (coleoptera: curculionidae): distribution of radioactivity in the principal lipid classes synthesized from c14-1-acetate. 19665969689
effects of the fall environment on the boll weevil in northeast mississippi. 19665976099
mating behavior of the female boll weevil. 19665976134
initial field tests with methyl parathion and epn in mexico against the boll weevil. 19665976145
lysozyme in eggs of the cotton boll weevil, anthonomus grandis boheman (coleoptera: curculionidae).lysozyme exhibiting bacteriolytic activity was found in boll weevil eggs at a concentration of 2.22 ng of lysozyme per boll weevil egg.19807371758
analysis of a vitellogenin gene of the mosquito, aedes aegypti and comparisons to vitellogenins from other organisms.a genomic clone of the aedes aegypti vitellogenin a1 gene was sequenced including 2015 bp of 5' untranscribed sequence, 6369 bp of open reading frame interrupted by two introns, and a short 3' untranslated region. primer extension was used to identify the transcription initiation site. the amino termini of the large and small subunits were located by n-terminal sequencing of vitellin purified from eggs. the length of the signal sequence and the position of the cleavage site between the two subun ...19957550249
expression of beta-galactosidase and luciferase in insect cell lines infected with a recombinant acmnpv.an acmnpv recombinant (ac-gal-luc) carrying the beta-galactosidase and luciferase genes under the control of the p10 and polyhedrin promoters, respectively, was used to study expression in nine insect cell lines. all acmnpv-permissive cell lines expressed both reporter genes with the coleopteran cell line, anthonomus grandis (age), producing the highest concentrations of beta-galactosidase (5.0 x 10(6) pg/ml) and luciferase (2.67 x 10(3) pg/ml). both enzymes were detected as early as 12 h postin ...19948069450
cholesterol oxidase: a potent insecticidal protein active against boll weevil larvae.the discovery of proteins that control insects is critical for the continued growth of the agricultural biotechnology industry. a highly efficacious protein that killed boll weevil (anthonomus grandis grandis boheman) larvae was discovered in streptomyces culture filtrates. the protein was identified as cholesterol oxidase (e.c. 1.1.3.6). purified cholesterol oxidase was active against boll weevil larvae at a concentration (lc50 = 20.9 micrograms/ml) comparable to the bioactivity of bacillus thu ...19938250897
proteolytic processing of the vitellogenin precursor in the boll weevil, anthonomus grandis.the soluble proteins of the eggs of the coleopteran insect anthonomus grandis boheman, the cotton boll weevil, consist almost entirely of two vitellin types with m(r)s of 160,000 and 47,000. we sequenced their n-terminal ends and one internal cyanogen bromide fragment of the large vitellin and compared these sequences with the deduced amino acid sequence from the vitellogenin gene. the results suggest that both the boll weevil vitellin proteins are products of the proteolytic cleavage of a singl ...19938347856
characterization and partial cloning of ecdysteroid receptor from a cotton boll weevil embryonic cell line.a cell line derived from the embryos of the cotton boll weevil, anthonomus grandis (brl-ag-2), was used to study morphological and biochemical responses to 20-hydroxyecdysone (20e). the cells respond to 10(-6) m 20e by inhibition of cell growth and enhanced production of some secreted proteins. crude nuclear extracts containing the ecdysteroid receptor complex proteins consisting of the ecdysteroid receptor (ecr) and ultraspiracle (usp) bound ponasterone a with a kd of 6.1 nm. bound radiolabeled ...19979131780
in vitro and in vivo host range of anticarsia gemmatalis multiple nuclear polyhedrosis virus.a clone of the wild type (wt) anticarsia gemmatalis multiple nuclear polyhedrosis virus agmnpv, derived from a geographical isolate (hondrina, brazil) and designated agmnpv-cl4-3a1, was used to determine the host range of this virus in six established lepidopteran cell lines: anticarsia gemmatalis (bcirl-ag-am1), helicoverpa zea (bcirl-hz-am1), heliothis virescens (bcirl-hv-am1), helicoverpa armigera (bcirl-ha-am1), trichoplusia ni (tn-cl1), bombyx mori (bmn), and a coleopteran cell line anthono ...19989542640
the effect of insecticides on learning in the africanized honey bee (apis mellifera l.).the present study was designed to examine the effects of endosulfan, decis, baytroid, and sevin on the learning ability of africanized honey bees (apis mellifera l.). although these insecticides were recommended by the government of brazil to control the cotton boll weevil, the effects on bees have been unknown. results of the present research show that: (1) bees readily consume each of the pesticides when placed in a sucrose solution; (2) the odors of the pesticides are not repellent to bees, a ...199910508901
synthesis of 2,2,4,4-tetramethyl-n,n'-bis(2,6-dimethylphenyl)cyclobutane-1,3-diimine , a unique compound from arundo donax, and its analogues to test their antifeedant activity against the boll weevil, anthonomus grandis.2,2,4,4-tetramethyl-n,n'-bis(2,6-dimethylphenyl) cyclobutane-1,3-diimine (1), which was isolated from the thai plant arundo donax as an antifeedant against the boll weevil (anthonomus grandis), and its analogues (9-13) were synthesized and shown to possess no remarkable antifeedant activity of practical interest.200010803974
lethal and sublethal effects of selected insecticides and an insect growth regulator on the boll weevil (coleoptera: curculionidae) ectoparasitoid catolaccus grandis (hymenoptera: pteromalidae).a laboratory culture of catolaccus grandis (burks), an ectoparasitoid of the boll weevil, anthonomus grandis grandis boheman, was exposed to lethal and sublethal doses of insecticides and an insect growth regulator using a spray chamber bioassay. materials tested were azinphos-methyl, endosulfan, fipronil, malathion, cyfluthrin, dimethoate, spinosad, methyl parathion, acephate, oxamyl, and tebufenozide. at full rates, spinosad was significantly less toxic to female c. grandis than other treatmen ...200010826176
role of ground trash volatiles in the selection of hibernation sites by boll weevils.volatiles play a large role in governing the behavior of boll weevils (anthonomus grandis boheman). they are attracted to cotton plants, and the female is sexually attracted to the male. the attracting compounds in both instances are terpenoids. primarily in the fall of the year, boll weevils seek hibernation sites in leaf trash, where they remain until the following spring or summer. in the present study, essential oils were prepared by steam distillation from several leaf samples known to be p ...200010956168
an asymmetric synthesis of (+)-grandisol, a constituent of the aggregation pheromone of the cotton boll weevil, via a kinetic resolution.a novel approach to the asymmetric synthesis of (+)-grandisol, (1r, 2s)-isopropenyl-1-methylcyclobutaneethanol, involves the use of catalytic kinetic resolution of a primary allylic alcohol, [(1rs, 5sr)-5-methylbicyclo[3.2.0]hept-2-en-2-yl] methanol. the allylic alcohol is prepared in four steps from simple achiral materials involving the use of a modified shapiro reaction. the resolved alcohol (95% ee) is then reduced in two steps to the corresponding methyl alkene, (1s,5r)-2,5-dimethylbicyclo[ ...200011073589
dispersal of the cotton boll weevil (coleoptera: curculionidae) in south america: evidence of rapd analysis.rapd technique provides useful information on the geographic origin and dispersal of the boll weevil anthonomus grandis in south america. nine populations from argentina, brazil, paraguay, mexico and usa were analyzed. weevils were captured on native plants (misiones province, argentina) and on cotton cultures, except the sample from the united states (usda laboratory-reared colony). a sample of the 'peruvian square weevil', a. vestitus, from ecuador, was included in the analysis in order to com ...200011138940
evaluation of individual components of plum odor as potential attractants for adult plum curculios.we evaluated olfactory attraction of overwintered plum curculio (pc) adults, conotrachelus nenuphar, to 16 individual volatile components of unripe plum odor in the laboratory using a still-air dual-choice bioassay system and in the field using baited cotton dental wicks attached to boll-weevil traps placed on the ground beneath the canopy of unsprayed apple trees. two compounds, ethyl isovalerate and limonene, were significantly attractive in both laboratory bioassays and field experiments. in ...200111382056
replication of chilo iridescent virus in the cotton boll weevil, anthonomus grandis, and development of an infectivity assay.the boll weevil, anthonomus grandis boheman, is a devastating pest of cotton. chemical pesticides are problematic due to relative lack of target specificity and resistance. microbial pesticides may provide viable alternatives because of their narrow host range. chilo iridescent virus (civ) is the type species for genus iridovirus, family iridoviridae: large, icosahedral cytoplasmic viruses containing a double-stranded dna genome. earlier work suggested that civ replicated in the boll weevil; how ...200111402862
karyotypic analysis of the cotton boll weevil, anthonomus grandis boheman.the diploid chromosome number of the cotton boll weevil, anthonomus grandis boheman, is 44. both c- and n-banding techniques of mitotic cells demonstrated constitutive heterochromatin in the p arm of the eight largest chromosomes, the p arm of the x chromosome, and the centromeric region of autosomal groups a-d. neither the y nor the group e autosomes appeared to contain constitutive heterochromatin. supernumerary chromosomes were not found in the boll weevil. restriction endonuclease banding of ...200011430485
expression and chloroplast targeting of cholesterol oxidase in transgenic tobacco plants.cholesterol oxidase represents a novel type of insecticidal protein with potent activity against the cotton boll weevil (anthonomus grandis grandis boheman). we transformed tobacco (nicotiana tabacum) plants with the cholesterol oxidase chom gene and expressed cytosolic and chloroplast-targeted versions of the chom protein. transgenic leaf tissues expressing cholesterol oxidase exerted insecticidal activity against boll weevil larvae. our results indicate that cholesterol oxidase can metabolize ...200111457962
components of male aggregation pheromone of strawberry blossom weevil, anthonomus rubi herbst. (coleoptera:curculionidae).the strawberry blossom weevil, anthonomus rubi, is a major pest of strawberries in the united kingdom and continental europe. as part of a project to develop noninsecticidal control methods, the pheromone system of this species was investigated. comparison of volatiles produced by field-collected, overwintering individuals of each sex led to identification of three male-specific compounds--(z)-2-(3,3-dimethylcyclohexylidene)ethanol, (cis)-1-methyl-2-(1-methylethenyl)cyclobutaneethanol, and 2-(1- ...200111504023
transcription and temporal cascade in chilo iridescent virus infected cells.chilo iridescent virus (civ) is the type species for genus iridovirus, and belongs to the family iridoviridae. members of this family are large, isometric, cytoplasmic dna viruses. our laboratory has established that civ replicates productively in the cotton boll weevil, anthonomus grandis. given the economic importance of this host and the dearth of knowledge on this virus, we have initiated host-virus interaction and molecular studies on civ. this report focuses on regulation of transcription ...200111765918
ecology and phenology of the boll weevil (coleoptera: curculionidae) on an unusual wild host, hibiscus pernambucensis, in southeastern mexico.the phenology and ecology of hibiscus pernambucensis arruda and its interaction and importance in maintaining populations of the boll weevil, anthonomus grandis boheman, were studied over a period of 3 yr in the soconusco region of the state of chiapas, mexico. h. pernambucensis is a small tree of neotropical distribution, restricted to lowland areas, and generally associated with halophytic vegetation. this species is found exclusively along the shores of brackish estuaries, in or near mangrove ...200111777042
correspondence of high levels of beta-exotoxin i and the presence of cry1b in bacillus thuringiensis.examination of 640 natural isolates of bacillus thuringiensis showed that the 58 strains (9%) whose supernatants were toxic to anthonomus grandis (coleoptera: curculionidae) produced between 10 and 175 micro g of beta-exotoxin i per ml. we also found that 55 (46%) of a sample of 118 strains whose culture supernatants were not toxic to a. grandis nevertheless produced between 2 and 5 micro g/ml. however, these amounts of beta-exotoxin i were below the threshold for detectable toxicity against thi ...200212200263
effects of kaolin-based particle film application on boll weevil (coleoptera: curculionidae) injury to cotton.this study examined a non-insecticidal tactic for suppressing boll weevil, anthonomus grandis grandis boheman, damage to cotton, gossypium hirsutum l. in cage assays, kaolin, a reflective white mineral, applied to excised cotton squares or to the cotton foliage, initially resulted in lower levels of boll weevil injury to squares than nontreated squares. boll weevil oviposition and feeding on kaolin-treated squares and squares on kaolin-treated cotton plants increased when nontreated squares and ...200212216817
paget's disease of bone in lancashire and arsenic pesticide in cotton mill wastewater: a speculative hypothesis.a 1974 survey of paget's disease of bone in 31 uk towns identified a cluster of six lancashire former cotton mill towns with the highest prevalence rates of the disease and noted a link with the cotton industry. the two highest-prevalence towns are situated on estuaries and are jointly the smallest of the six by the size of their former cotton industry. the two lowest-prevalence towns of the six are highest above sea level and jointly the largest by the size of their former cotton industry. this ...200212231419
efficacy of the organic-certified insecticide diatect ii against the boll weevil (anthonomus grandis) in cotton.the efficacy of the organic insecticide diatect ii against boll weevil (anthonomus grandis boheman) in cotton (gossypium hirsutum l) in the lower rio grande valley of texas were assessed in small-plot field trials and greenhouse cage tests using azinphos-methyl treatments as a standard for comparison. plastic sheets were placed in the furrows of the treated plots to retrieve boll weevils which dropped from the plants after being killed by the insecticides. samples of live weevils taken by a trac ...200212400443
susceptibility of the boll weevil to steinernema riobrave and other entomopathogenic nematodes.the susceptibility of the boll weevil (bw), anthonomus grandis boheman, to steinernema riobrave and other nematode species in petri dishes, soil (hidalgo sandy clay loam), and cotton bolls and squares was investigated. third instar weevils were susceptible to entomopathogenic nematode (en) species and strains in petri dish bioassays at 30 degrees c. lower lc(50)'s occurred with s. riobrave tx- 355 (2 nematodes per weevil), s. glaseri nc (3), heterorhabditis indicus hom-1 (5), and h. bacteriophor ...200312676555
effects of black-eyed pea trypsin/chymotrypsin inhibitor on proteolytic activity and on development of anthonomus grandis.the cotton boll weevil anthonomus grandis (boheman) is one of the major pests of cotton (gossypium hirsutum l.) in tropical and sub-tropical areas of the new world. this feeds on cotton floral fruits and buds causing severe crop losses. digestion in the boll weevil is facilitated by high levels of serine proteinases, which are responsible for the almost all proteolytic activity. aiming to reduce the proteolytic activity, the inhibitory effects of black-eyed pea trypsin/chymotrypsin inhibitor (bt ...200312737983
molecular cloning of alpha-amylases from cotton boll weevil, anthonomus grandis and structural relations to plant inhibitors: an approach to insect resistance.anthonomus grandis, the cotton boll weevil, causes severe cotton crop losses in north and south america. here we demonstrate the presence of starch in the cotton pollen grains and young ovules that are the main a. grandis food source. we further demonstrate the presence of alpha-amylase activity, an essential enzyme of carbohydrate metabolism for many crop pests, in a. grandis midgut. two alpha-amylase cdnas from a. grandis larvae were isolated using rt-pcr followed by 5' and 3' race techniques. ...200312744224
a hexamerin protein, agsp-1, is associated with diapause in the boll weevil(1).the objective of this research was to identify a reliable biochemical indicator for diapause (dormancy) in the boll weevil, anthonomus grandis. hemolymph polypeptides from reproductive and diapausing weevils were compared using denaturing sodium dodecyl sulpfate (sds)-polyacrylamide gel electrophoresis (page). a 77-kda protein, which proved to be a hexamerin (agsp-1), strongly correlated with morphological diapause characters in both male and female adult weevils. n-terminal sequence analysis id ...200212770051
effects of routine late-season field operations on numbers of boll weevils (coleoptera: curculionidae) captured in large-capacity pheromone traps.flat and cylindrical adhesive boll weevil, anthonomus grandis grandis boheman (coleoptera: curculionidae), pheromone traps captured significantly more (p < or = 0.05) boll weevils than the hercon (hercon environmental, emigsville, pa) trap during the late cotton-growing season, and larger adhesive areas were associated with higher captures; a flat plywood board collected the most boll weevils because it had the largest surface area. the flat board trap, chosen for measuring large late-season adu ...200312852605
effect of ulv malathion use in boll weevil (coleoptera: curculionidae) eradication on resistance in the tarnished plant bug (heteroptera: miridae).tarnished plant bugs, lygus lineolaris (palisot de beauvois), from regions 1, 2, and 3 of the boll weevil, anthonomous grandis boheman, eradication program in mississippi were collected from wild hosts and tested for malathion resistance during the spring and fall of 2000 and 2001. plant bugs were also tested in region 1 in late-july and october of 1999, just before and after multiple applications of ultra-low-volume (ulv) malathion were used for reproduction-diapause control of boll weevils in ...200312852634
effects of soybean kunitz trypsin inhibitor on the cotton boll weevil (anthonomus grandis).the cotton boll weevil, anthonomus grandis, is an economically important pest of cotton in tropical and subtropical areas of several countries in the americas, causing severe losses due to their damage in cotton floral buds. enzymatic assays using gut extracts from larval and adult boll weevil have demonstrated the presence of digestive serine proteinase-like activities. furthermore, in vitro assays showed that soybean kunitz trypsin inhibitor (skti) was able to inhibit these enzymes. previously ...200414697273
occurrence and linkage between secreted insecticidal toxins in natural isolates of bacillus thuringiensis.little is known about the occurrence and linkage between secreted insecticidal virulence factors in natural populations of bacillus thuringiensis (bt). we carried out a survey of 392 bt strains isolated from various samples originating from 31 countries. the toxicity profile of the culture supernatants of these strains was determined individually against anthonomus grandis (coleoptera) and spodoptera littoralis (lepidoptera). we analyzed beta-exotoxin i production and searched for the genes enco ...200314756535
boll weevil (coleoptera: curculionidae) bait sticks: toxicity and malathion content.assays of malathion content and toxicity to boll weevil, anthonomus grandis grandis bohemian, were conducted on boll weevil bait sticks, now marketed as boll weevil attract and control tubes (bwacts; plato industries, houston, tx). in general, the longer bwacts were in the field, the lower the mortality of weevils that were exposed to them. bioassays of weevil mortality correlated with hexane washes of bwact surfaces showed highly variable mortality when surface malathion fell below approximatel ...200314994795
early-season colonization patterns of the boll weevil (coleoptera: curculionidae) in central texas cotton.it is commonly believed that colonization of early-season cotton, gossypium hirsutum l., by overwintered boll weevils, anthonomus grandis grandis boheman, is concentrated on field margins. however, supporting experimental evidence is not available. in 1999 and 2000, we examined colonization patterns of overwintered boll weevils in central texas cotton on the bases of adult collections by a pneumatic sampler and hand collections of abscised infested squares. samples were taken from sites arranged ...200314994797
evaluation of kill strips on boll weevil (coleoptera: curculionidae) mortality in pheromone traps and impact on weevil escape.a field study examined the temporal patterns of boll weevil mortality provided by two commercially available kill strips, hercon vaportape ii and plato insecticide strip, and to evaluate the impacts of these devices on weevil escape from traps. both types of kill strips produced similar levels of weevil mortality with the exception of the last two inspection intervals (30 and 46 h after continual exposure to kill strips). at these intervals, the plato strip produced significantly higher mortalit ...200314994800
a kunitz-type inhibitor of coleopteran proteases, isolated from adenanthera pavonina l. seeds and its effect on callosobruchus maculatus.the cowpea weevil callosobruchus maculatus is one of the major pests of vigna unguiculata cowpea. digestion in the cowpea weevil is facilitated by high levels of cysteine and aspartic acid proteinases. plants synthesize a variety of molecules, including proteinaceous proteinase inhibitors, to defend themselves against attack by insects. in this work, a trypsin inhibitor (apti) isolated from adenanthera pavonina seeds showed activity against papain. the inhibition of papain by apti was of the non ...200415113152
effects of burial and soil condition on postharvest mortality of boll weevils (coleoptera: curculionidae) in fallen cotton fruit.effects of soil condition and burial on boll weevil, anthonomus grandis grandis boheman, mortality in fallen cotton, gossypium hirsutum l., fruit were assessed in this study. during hot weather immediately after summer harvest operations in the lower rio grande valley of texas, burial of infested fruit in conventionally tilled field plots permitted significantly greater survival of weevils than in no-tillage plots. burial of infested squares protected developing weevils from heat and desiccation ...200415154462
deterrent effects of four neem-based formulations on gravid female boll weevil (coleoptera: curculionidae) feeding and oviposition on cotton squares.three commercial neem-based insecticides, agroneem, ecozin, and neemix, and a neem seed extract formulation, bitters, containing 1,036, 16,506, 471, and 223 microg/ml azadirachtin, respectively, were assessed for feeding and oviposition deterrence against gravid female boll weevils, anthonomus grandis grandis boheman, in the laboratory. in choice assays, excised cotton squares dipped in the separate formulations were first physically contacted by the weevils' tarsi or antennae fewer times than n ...200415154463
genetic structuring of boll weevil populations in the us based on rapd markers.abstract randomly amplified polymorphic dna (rapd) analysis was performed to infer the magnitude and pattern of genetic differentiation among boll weevil populations from eighteen locations across eight us states and north-east mexico. sixty-seven reproducible bands from six random primers were analysed for genetic variation within and between weevil populations. genetic and geographical distances among all populations were positively correlated, reflecting a pattern of isolation by distance wit ...200415157230
molecular cloning of a cysteine proteinase cdna from the cotton boll weevil anthonomus grandis (coleoptera: curculionidae).the cotton boll weevil (anthonomus grandis) causes severe cotton crop losses in north and south america. this report describes the presence of cysteine proteinase activity in the cotton boll weevil. cysteine proteinase inhibitors from different sources were assayed against total a. grandis proteinases but, unexpectedly, no inhibitor tested was particularly effective. in order to screen for active inhibitors against the boll weevil, a cysteine proteinase cdna (agcys1) was isolated from a. grandis ...200415215586
potential for transport of boll weevils (coleoptera: curculionidae) to the cotton gin within cotton modules.there is concern that cotton gins located in boll weevil, anthonomus grandis grandis boheman, eradication zones serving customers in adjacent infested zones may serve as a site for boll weevil reintroductions if weevils are transported alive inside cotton modules. we surveyed fields in three distinct areas of texas and found that weevils can be present in large numbers in cotton fields that have been defoliated and desiccated in preparation for harvest, both as free adults and as immatures insid ...200415279274
a diverse family of serine proteinase genes expressed in cotton boll weevil (anthonomus grandis): implications for the design of pest-resistant transgenic cotton plants.fourteen different cdna fragments encoding serine proteinases were isolated by reverse transcription-pcr from cotton boll weevil (anthonomus grandis) larvae. a large diversity between the sequences was observed, with a mean pairwise identity of 22% in the amino acid sequence. the cdnas encompassed 11 trypsin-like sequences classifiable into three families and three chymotrypsin-like sequences belonging to a single family. using a combination of 5' and 3' race, the full-length sequence was obtain ...200415350610
effects of insecticides and defoliants applied alone and in combination for control of overwintering boll weevil (anthonomus grandis; coleoptera: curculionidae)--laboratory and field studies.in laboratory, greenhouse and field tests, we determined the effects of combining full rates of the defoliants tribufos and thidiazuron and the herbicide thifensulfuron-methyl with half rates of the insecticides lambda-cyhalothrin or azinphos-methyl, and the combination of tribufos and thidiazuron, both in half rates, on mortality of the boll weevil, anthonomus grandis grandis boheman and on the quality of defoliation. tribufos, 0.47 kg ha(-1) and tribufos, 0.235 kg ha(-1) + thidiazuron, 0.125 k ...200415382499
boll weevil (coleoptera: curculionidae) survival through the seed cotton cleaning process in the cotton gin.there is concern that gins located in boll weevil, anthonomus grandis grandis boheman, eradication zones may become points of reintroduction when they process cotton grown in a neighboring infested area. we estimated boll weevil survival through two typical machine sequences used in commercial cotton gins to clean and dry the seed cotton in advance of the gin stand, as well as separately through two incline cylinder cleaners or one or two tower dryers operating at different temperatures. large n ...200415384344
influence of cotton fruit stages as food sources on boll weevil (coleoptera: curculionidae) fecundity and oviposition.boll weevil, anthonomus grandis grandis boheman (coloeoptera: curculionidae), populations in the lower rio grande valley of texas increase strongly during the squaring stage, and preemptive insecticide sprays at the pinhead square stage were designed to capitalize on that association. laboratory assays showed that cotton plant volatiles, or leaves as a food source, do not elicit egg production in wild weevils. boll weevils fed on large (5.5-8-mm diameter) squares for 7 d resulted in > or = 3.8-f ...200415384345
molecular characterization of brevibacillus laterosporus and its potential use in biological control.thirty-three strains of brevibacillus laterosporus, including three novel strains isolated from brazilian soil samples, were examined for genetic variability by the use of different pcr-based methods. molecular markers that could characterize bacterial strains with regards to their pathogenic potential were investigated. in addition, toxicity was assessed by the use of insects belonging to the orders lepidoptera and coleoptera and the mollusk biomphalaria glabrata. among the targets tested, biom ...200415528531
boll weevil (coleoptera: curculionidae) survival through cotton gin trash fans.there is concern that cotton gins may serve as loci for reintroduction of boll weevils, anthonomus grandis grandis boheman, to eradicated or suppressed zones when processing weevil-infested cotton from neighboring zones. previous work has shown that virtually all weevils entering the gin in the seed cotton will be removed before they reach the gin stand. those not killed by the seed cotton cleaning machinery will be shunted alive into the trash fraction, which passes through a centrifugal trash ...200415568350
toxicity to cotton boll weevil anthonomus grandis of a trypsin inhibitor from chickpea seeds.cotton (gossypium hirsutum l.) is an important agricultural commodity, which is attacked by several pests such as the cotton boll weevil anthonomus grandis. adult a. grandis feed on fruits and leaf petioles, reducing drastically the crop production. the predominance of boll weevil digestive serine proteinases has motivated inhibitor screenings in order to discover new ones with the capability to reduce the digestion process. the present study describes a novel proteinase inhibitor from chickpea ...200515649779
potential for escape of live boll weevils (coleoptera: curculionidae) into cottonseed, motes, and cleaned lint at the cotton gin.reintroductions of the boll weevil, anthonomus grandis grandis boheman, into areas of the united states where it has been eradicated or suppressed are very expensive to mitigate. there is concern that a cotton gin in an eradication zone may serve as a site of boll weevil reintroductions when processing cotton harvested in a neighboring infested zone. similarly, there is a question whether weevil-free areas can safely import gin products, such as cottonseed and baled lint, from infested areas wit ...200415666727
jh iii production, titers and degradation in relation to reproduction in male and female anthonomus grandis.juvenile hormone (jh) is necessary for the production of vitellogenin (vg) in the boll weevil, anthonomus grandis. occurrence of vg in this species is typically restricted to reproductively competent females, and is not detected in untreated males. however, the jh analog, methoprene stimulates vg production in intact males and in the isolated abdomens of both male and female boll weevils (where in each case no vg is detected without treatment), suggesting that males are competent to produce vg b ...200515890186
in vivo bioinsecticidal activity toward ceratitis capitata (fruit fly) and callosobruchus maculatus (cowpea weevil) and in vitro bioinsecticidal activity toward different orders of insect pests of a trypsin inhibitor purified from tamarind tree (tamarindus indica) seeds.a proteinaceous inhibitor with high activity against trypsin-like serine proteinases was purified from seeds of the tamarind tree (tamarindus indica) by gel filtration on shephacryl s-200 followed by a reverse-phase hplc vidac c18 tp. the inhibitor, called the tamarind trypsin inhibitor (tti), showed a mr of 21.42 kda by mass spectrometry analysis. tti was a noncompetitive inhibitor with a ki value of 1.7 x 10(-9) m. in vitro bioinsecticidal activity against insect digestive enzymes from differe ...200515913299
molecular cloning and expression of an alpha-amylase inhibitor from rye with potential for controlling insect pests.alpha-amylase inhibitors have important roles in plant defense mechanisms, particularly against insects, and several of these inhibitors have been expressed in different crops to increase their resistance to particular insects. in this work, we report the cloning and expression of a gene encoding for a new alpha-amylase inhibitor (biii) from rye (secale cereale) seeds. the biii gene contains 354 nucleotides that encode for 118 amino acids sequence. a 313 bp fragment of the gene was expressed in ...200516003953
size-dependent feeding and reproduction by boll weevil (coleoptera: curculionidae).the considerable variation in adult size of the boll weevil, anthonomus grandis grandis boheman, has been well documented, but the influences of adult size on reproductive rate are not known. we examined the relationship between the size of boll weevils and their feeding and oviposition. weevils weighed to the nearest milligram were grouped into five categories based on pupal weight: < or =5, 6-10, 11-15, 16-20, and >20 mg. numbers of lifetime punctures produced in flower buds (squares) of cotto ...200516022302
effects of planting dates on boll weevils (coleoptera: curculionidae) and cotton fruit in the subtropics.the effects of planting dates 2-3-wk apart on boll weevil, anthonomus grandis grandis boheman (coleoptera: curculionidae), field-level populations, and feeding and oviposition damage to cotton, gossypium hirsutum l., squares and bolls, were studied during 2002 and 2003 in the lower rio grande valley of texas. squares were 44-56% more abundant in some later planted treatments than in the earlier planted treatments, but mean cumulative numbers of oviposition- and feeding-damaged squares were 2.7 - ...200516022308
purification of a 6.5 kda protease inhibitor from amazon inga umbratica seeds effective against serine proteases of the boll weevil anthonomus grandis.a 6.5 kda serine protease inhibitor was purified by anion-exchange chromatography from the crude extract of the inga umbratica seeds, containing inhibitor isoforms ranging from 6.3 to 6.7 kda and protease inhibitors of approximately 19 kda. the purified protein was characterized as a potent inhibitor against trypsin and chymotrypsin and it was named i. umbratica trypsin and chymotrypsin inhibitor (iutci). maldi-tof spectra of the iutci, in the presence of dtt, showed six disulfide bonds content, ...200516101400
responses of flea beetle phyllotreta cruciferae to synthetic aggregation pheromone components and host plant volatiles in field trials.male-specific compounds, previously identified from phyllotreta cruciferae and synthesized or isolated from natural sources, attracted both sexes of the beetle in field trials and therefore function as components of a male-produced aggregation pheromone. six field experiments of 7 to 10 d duration each were conducted over 2 yr using modified boll weevil traps and two doses of pheromone. treatments containing two doses of allyl isothiocyanate (aitc), a breakdown product of glucosinolates in brass ...200516222810
relationships of different cotton square sizes to boll weevil (coleoptera: curculionidae) feeding and oviposition in field conditions.feeding and oviposition preferences of the boll weevil, anthonomus grandis grandis boheman, for four different cotton square size classes in field conditions of the lower rio grande valley of texas were studied during 2002 and 2003. percentages of large (5.5-8-mm-diameter) squares used for oviposition and feeding were greater than pinhead or match-head squares. the preference for large squares as food and associated accelerated fecundity explain the substantial boll weevil population buildups th ...200516334326
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