Publications

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[the role of the blatella germanica in transmission of infectious agents (author's transl)]. 1975124222
cockroaches as vectors of sarcocystis muris and of other coccidia in the laboratory.to assess the roles of the german cockroach (blatella germanica) and the american cockroach (periplaneta americana) in the transmission of sarcocystis muris and of 3 other coccidia of cats-toxoplasma gondii, isospora felis, and isospora rivolta, cockroaches exposed to feces containing these coccidia were periodically fed to mice, as was a portion of the fecal matter. sarcocystis muris sporocysts, which in feces remained infectious for at least 20 days, were also transmitted to mice by p. america ...1978417163
study on the presence of salmonella and other pathogenic bacteria in cockroaches on ocean-going ships. i. basic investigations on isolation of bacterial strains from blattella germanica (l.). 1979497502
study on the possibility of adopting a complex method in blattella germanica (l.) control on board ships.campaings to control the german cockroach, blattela germanica, were conducted at 14-day intervals over a period of 5.5 months on two merchant vessels of similar size and type. on one ship only the chemical "gertoks", carbamate insecticide containing 1% propoxur was applied, and on the second ship two preparations simultaneously, a chemical and a biological--"biotrol 25 w" containing bacillus thuringiensis. - in order to assess the success of the control, the extent of infestation in the accomoda ...1979506564
[studies of the sensitivity to the usual domestic insecticides on insects of the anopheles maculipennis group, and musca domestica, cimex lectularius and blattella germanica species].the present paper was designed to the study of the degree of sensitivity of mosquitoes, flies, bedbugs and cockroaches to organochloride and organophosphoric insecticides produced in romania. testing of the sensitivity to insecticides of these species, important as transmitters of human pathogens, showed the effectiveness of the products, which induced a high mortality rate in relatively small concentrations. against anopheles labranchiae atroparvus, the insecticides detox, carbetox and lindatox ...1978694334
[nematodes of the genus spirura, parasites of tupaia and nycticebus in malaysia (author's transl)].morphological study of two spirura parasites of the oesophageal and the gastric wall of tupaia and nycticebus in malaysia. -- spirura malayensis n. sp. is found both in tupaia in the district of selangor (west malaysia) and in nycticebus coucang in borneo. its very primitive characteristics relate it to s. diplocyphos chabaud, brygoo and petter, 1965, parasite of lemurs from madagascar. its larval development was obtained experimentally in blatella germanica. -- spirura aurangabadensis (ali and ...1975818936
the german cockroach, blatella germanica l, from the human ear.a case of invasion of the human ear by the german cockroach blattella germanica (l) in mash'had, khorassan, iran is described. as far as the author is aware this is the first such case recorded and published from iran.1977917582
studies of the infection of the cockroaches (blattella germanica[l.]) with bac. thuringensis.the results of an experimental infection of the cockroaches blattella germanica (l.) with some trade preparations containing bac. thuringensis spores are presented. biological studies supported by bacteriological and histological examinations of the excised intestines of the above insects have shown that the microorganisms studied are pathogenic for the cockroaches. this effect might be eventually applied as a biological method of eradication of the cockroaches in such environments, where chemic ...19751189795
insect peptide hormones, an overview of the present literature.a comprehensive overview of the recent state of the art of insect peptide hormones with chemical structures is presented. an increased interest in insect neuropeptides and dynamic development of that research area has been influenced by a rapid improvement of instrumentation necessary for isolation and structural characterization. several research teams have studied the relationships between biological properties of insect and vertebrate peptide hormones. thus hormones from the akh family can be ...19921353067
nosocomial fungal infections--study of the possible role of cockroaches (blattella germanica) as vectors.a variety of fungi of medical importance were recovered from cockroaches (blattella germanica) collected from hospital wards (159) and residential areas (128) in different seasons. the fungi isolated were various species of candida, rhizopus, mucor, alternaria and aspergillus. candida spp. were the fungi isolated in highest percentage from hospital and residential area.19921356305
[biological detector of weak cosmic fields].possibility of use of biological object as a detector of faint cosmophysical fields is discussed. the signals of free induction and electric echo caused by a short-term effect of light with an intensity of approximately 0.2 mwt/sm2 on biological systems were observed. the experiments with blatella germanica, muska domestica were performed in vivo and with red and green seaweeds and yeast cells in vitro. the signals were detected by means of the dielectric permeability measurement in a dynamic re ...19921420418
cockroaches as carriers of bacteria in multi-family dwellings.the potential risk of bacterial dissemination due to the presence of cockroaches (blattella germanica, blattellidae) in low-income flats was investigated. cockroaches can carry a great variety of bacterial species; we identified 30 different species from 52 different flats. klebsiella oxycytoca, k. pneumoniae and enterobacter cloacae were the most frequently found. pathogenic and potentially pathogenic bacteria represented 54% of all the bacterial identifications. bacteria were carried either on ...19921468532
susceptibility of cockroaches (dictyoptera: blattellidae, blattidae) to infection by steinernema carpocapsae.the susceptibility of american cockroaches, periplaneta americana (l.); smoky brown cockroaches, p. fuliginosa (serville); oriental cockroaches, blatta orientalis l.; german cockroaches, blattella germanica (l.); and brownbanded cockroaches, supella longipalpa (f.), to steinernema carpocapsae weiser (all strain) was evaluated under laboratory conditions. a 1-ml water suspension containing 500,000 nematodes was placed on filter paper in a petri dish or the pad of a bait station. german, brown-ban ...19921517508
maternal and paternal nitrogen investment in blattella germanica (l.) (dictyoptera; blattellidae).the investment of nitrogenous materials by female and male german cockroaches blattella germanica (l.) into their progeny was examined. adult females maintained on dog food invested 34% of their dry mass and 26% of their nitrogen into an oothecae during their first gonadotrophic cycle. females maintained on a low- (5%) protein diet and injected simultaneously with [3h]leucine and [14c]hypoxanthine incorporated less [3h]leucine-derived radiolabel in their oothecae than those on a dog food diet (2 ...19921552280
biological response of german cockroaches fed diets containing allopurinol.the acceptability of dietary allopurinol to german cockroaches, blattella germanica (l.), was assessed. in diet choice tests between rat chow with or without 0.1% w/w allopurinol there were no significant differences in nymphal development, feeding duration, visits or consumption. the lt50 of cockroaches fed a choice of diets was 3 weeks greater (7.88) than those fed non-choice a 0.1% allopurinol diet. female cockroaches provided a choice of diets aborted a significantly greater percentage of th ...19921600230
differential development and reproduction of the german cockroach (dictyoptera: blattellidae) on three laboratory diets.development of nymphs and oocyte maturation in adults were examined in blattella germanica (l.) reared on three commercial diets. nymphs fed rat food developed significantly faster than nymphs fed two commercial dog foods. similarly, oocytes matured more quickly in adult females that were raised on rat food than in females raised on dog food. nymphal development and oocyte maturation were slower in insects that were fed whole dog food pellets than in insects fed ground dog food, suggesting that ...19921619105
skin test reactivity to whole body and fecal extracts of american (periplaneta americana) and german (blatella germanica) cockroaches in atopic asthmatics.forty-six atopic asthmatic subjects aged 3 to 58 years attending the allergy clinic of a university hospital were evaluated for prick skin test reactivity with commercially available extracts of cockroach (cce) and house dust (hd). additionally, skin testing was performed with american cockroach whole body (awbe) and fecal extracts (afe) as well as german cockroach whole body (gwbe) and fecal extracts (gfe) prepared in our laboratory. commercial cockroach extract was prepared from american, germ ...19911750718
distribution of oriental and german cockroaches, blatta orientalis and blattella germanica (dictyoptera), in the united kingdom.results are presented of a survey carried out by rentokil ltd on the distribution of the oriental cockroach blatta orientalis l. and the german cockroach blattella germanica l. in the united kingdom. the known ranges of both species are increased considerably by the findings of the survey, with several new vice-county records for scotland (including the western isles), england and wales. the frequency at which blatta orientalis was observed in outdoor habitats may indicate that this species some ...19911773117
toxicity of abamectin to cockroaches (dictyoptera: blattellidae, blattidae).abamectin was fed to german cockroaches, blattella germanica (l.), in non-choice tests. lt50s and lc50s were estimated by probit analysis. the lt50s for the german cockroach ranged from 4.4 to 1.7 d for males, from 9.0 to 2.4 d for females, and from 4.4 to 1.6 d for nymphs for bait concentrations of abamectin between 0.0025 and 0.0500%. the lc50s of abamectin were 0.0110 and 0.0040% from males, 0.0240 and 0.0090% for females, and 0.0200 and 0.0080% for nymphs at 3 and 6 d, respectively. the lt50 ...19911787219
cockroaches (blattella germanica) as carriers of microorganisms of medical importance in hospitals.a study was conducted to isolate and identify microorganisms of medical importance from cockroaches (blattella germanica) and to ascertain their vector potential in the epidemiology of nosocomial infections. bacteria, fungi and parasites of medical importance were isolated and identified. important bacterial pathogens responsible for wound infections, were further studies by antibiograms. one hundred and fifty-eight out of 159 (99.4%) cockroaches collected from hospital (test) and 113 out of 120 ...19911879483
shared allergenic activity in asian (blattella asahinai), german (blattella germanica), american (periplaneta americana), and oriental (blatta orientalis) cockroach species.whole-body extracts of the feral and peridomestic asian cockroach (blattella asahinai) and the three domestic cockroach species, german (blattella germanica), american (periplaneta americana), and oriental (blatta orientalis), were compared allergenically using an ige serum pool from 4 german cockroach sensitive individuals. in crossover radioallergosorbent inhibition analysis, the asian cockroach shared allergenic activity primarily with the german cockroach polymer and to a lesser extent with ...19902242929
role of insects as inhalant allergens in bronchial asthma with special reference to the clinical characteristics of patients.the whole body extracts (wbes) of 13 common insects from delhi, namely musca domestica (house fly); sitophilus oryzae (rice weevil); callosobrochous maculatus (pulse beetle); anopheles stephensi, culex quinquefasciatus and aedes aegypti (mosquitoes); blattella germanica and periplaneta americana (cockroaches: male, female and nymph); spodoptera litura and heliothis armigera (moths), were prepared to evaluate their allergenic significance in patients with allergic bronchial asthma. intradermal (i ...19902253082
primary structures of hypertrehalosaemic neuropeptides isolated from the corpora cardiaca of the cockroaches leucophaea maderae, gromphadorhina portentosa, blattella germanica and blatta orientalis and of the stick insect extatosoma tiaratum assigned by tandem fast atom bombardment mass spectrometry.hypertrehalosaemic peptides were isolated by reversed-phase high-performance liquid chromatography from corpora cardiaca of four species of cockroaches (leucophaea maderae, gromphadorhina portentosa, blattella germanica, and blatta orientalis) and one stick insect species (extatosoma tiaratum), and their primary sequences were assigned by collision-induced decomposition tandem fast atom bombardment mass spectrometry (fabms/cid/ms). the members of the cockroach families blaberidae (l. maderae and ...19902340112
catecholamines and related o-diphenols in cockroach hemolymph and cuticle during sclerotization and melanization: comparative studies on the order dictyoptera.catecholamines and related o-diphenols extracted from the cuticle and hemolymph of adult cockroaches during sclerotization and pigmentation of the cuticle were analyzed by reverse phase hplc with electrochemical detection. at ecdysis, dopamine (da) o-conjugates predominated in the hemolymph of periplaneta americana, p. australasiae, p. fuliginosa, p. brunnea, and blatta orientalis (blattidae); blattella germanica (blattellidae); and gromphadorhina portentosa and blaberus craniifer (blaberidae). ...19902391402
identification of a german cockroach-specific allergen by human ige and rabbit igg.to further study the immunology of insect hypersensitivity, we identified and partially characterized the principal allergens in whole body german cockroach (wbgcr) (blattella germanica) and compared this extract to whole body antigens prepared from other insects. wbgcr extract was fractionated over a calibrated sephadex g-200 column; peak allergenic activity was contained in fraction 3 (gcr3), containing components with apparent molecular weights ranging from 12,500 to 75,000 daltons. the antig ...19882462543
bacterial pathogens isolated from cockroaches trapped from paediatric wards in peninsular malaysia.a survey was conducted in 4 paediatric wards in malaysia to determine the distribution of various species of cockroaches and to examine their gut contents for bacteria. cockroaches were trapped from food dispensing areas (kitchens), store rooms, cupboards and open wards. 104 cockroaches were caught, consisting of periplaneta americana (67.3%), blattella germanica (26%), p. brunnea (4.8%), and supella longipalpa (1.9%). bacteria were isolated from all cockroaches except 3 p. americana. many bacte ...19892603192
cockroaches as vectors of pathogenic bacteria.one hundred and thirty two cockroaches of species blattella germanica--96 from hospital ward (test group) and 36 from residential areas (control group) were caught during nov. 1985 to nov. 1986. a variety of pathogenic and non-pathogenic bacteria were isolated from test and control group of insects. pseudomonas aeruginosa, staphylococcus aureus, streptococcus faecalis, and micrococci were isolated only from the test group of cockroaches. a high percentage (98.95 per cent) of test cockroaches wer ...19892638379
arthropod immune system. v. activated immunocytes (granulocytes) of the german cockroach, blattella germanica (l.) (dictyoptera: blattellidae) show increased number of microtubules and nuclear pores during immune reaction to foreign tissue.the german cockroach, blattella germanica (l.) (dictyoptera: blattellidae) has two major immunocytes (blood cells) (granulocytes (grs) and plasmatocytes). the grs participate both in encapsulation and phagocytosis of nonself tissue. structurally, the grs are flattened and discoid, and contain, among other organelles, microtubules that are arranged in the form of a bundle in their peripheral region in the plane of flattening. if one implants a foreign tissue in the cockroach's abdomen, the grs be ...19883180259
antigenic/allergenic characterization of american and german cockroach extracts.cockroach allergens have been implicated as clinically significant sensitizing agents in the induction/exacerbation of "urban asthma." in the present study, approximately 50% of atopic, predominantly inner-city residents had immediate wheal-and-flare cutaneous reactivity to a commercial american cockroach whole body extract. crude whole body extracts were prepared in our laboratory from both american (periplaneta americana) and german (blatella germanica) cockroach species. crossed immunoelectro ...19883346487
detection of ige antibodies to a wide range of insect species in subjects with suspected inhalant allergies to insects.sera obtained from subjects diagnosed on the basis of case history and skin tests as having inhalant allergies to insects, were tested for the presence of ige antibodies to antigens from the house fly musca domestica, a blowfly calliphora stygia, the common clothes moth tineola bisselliella, warehouse moth ephestia cautella, cockroach blattella germanica, carpet beetle anthrenus verbasci and silverfish ctenolepisma longicaudata. approximately one-third of the sera reacted with extracts from all ...19883350607
[occurrence, distribution and control of cockroaches in the east german district of rostock].the german cockroach (blattella germanica) is the prevalent species in the district of rostock. there was also an occurrence of blatta orientalis and, in one case, of periplaneta australasiae. periplaneta americana is occasionally introduced from warmer regions of the globe via containers. extent and intensity of infestation of many buildings has increased making large-scale control measures necessary. most control measures have been taken in food industry enterprises. the quantity of cockroach ...19873605784
[sensitivity of blattella germanica (l.) from the polish merchant navy ships to carbamate compounds]. 19863660830
[importance of animal epithelia as house dust allergens].among sera from 379 patients with atopy tested with the radio-allergo-sorbent test (rast) for domestic dust and house-dust mite (dermatophagoides pteronyssinus), 104 were positive only for house dust. positive results for both house dust and house-dust mites were obtained in 130, while in 145 the rast result was positive only for house-dust mites. 104 sera, negative for house-dust mites, were tested by rast for specific ige against epithelia from cats, dogs, guinea-pigs, horses and cattle, as we ...19854017894
[sensitivity of blatella germanica (l.) and blatta orientalis l. to some insecticides in czechoslovakia]. 19724125442
experimental studies on the epidemiological importance of the cockroach, blattella germanica (l.), in streptococcal infections on ships. 19734145999
[microsporidosis in natural populations of cockroaches, blattella germanica l. and blatta orientalis l]. 19734205724
larval-specific serum protein in the order dictyoptera i. immunologic characterization in larval blattella germanica and cross-reaction throughout the order. 19744217682
[effectiveness of chlorophos bait with ammonium carbonate in the control of roaches (blatta orientalis l. and blattella germanica l.)]. 19744459687
[blatella germanica (l.) in the epidemiology of streptococcal infections among sailors]. 19724574214
[infection of blatella germanica (l.) intestines with streptococci group a in histological sections]. 19724574215
[duration of persistance and excretion of shigella flexneri and sonnei by cockroaches, blattella germanica l. in individual dosed feeding]. 19734592943
[experience in the control of synanthropic cockroaches, blatta orientalis and blattella germanica l. in some town districts]. 19734782413
[infestation and possibility of a vibrio carrier state in oriental (blatta orientalis l.) and common (blatella germanica l.) cockroaches]. 19734783463
[epidemiological role of blattella germanica (l.) in amebiasis]. 19816275613
pterygodermatites nycticebi (nematoda: spirurida) in golden lion tamarins.pterygodermatites nycticebi (syn rictularia nycticebi), a spirurid nematode first described in the slow loris (nycticebus coucang), recently has been associated with morbidity and mortality in the golden lion tamarin (leontopithecus rosalia rosalia) collection at the national zoological park. adult worms were found in the lumen of the small intestine with their anterior ends embedded in the mucosa. larvae, when present, were deeper in the submucosa. a few heavily infected animals developed profo ...19836406763
laboratory studies on the susceptibility of blattella germanica and periplaneta americana to bacillus thuringiensis var. israelensis. 19846523178
cockroach larval-specific protein, a tyrosine-rich serum protein.larval-specific protein (lsp) is the most abundant protein in the hemolymph of cockroaches shortly before molting, but is rapidly cleared from the hemolymph during the molt (kunkel, j. g., and lawler, d. m. (1974) comp. biochem. physiol. 47b, 697-710). blatta orientalis lsp was purified by sedimentation in preparative sucrose gradients followed by 2-hydroxypropylamino-cellulose anion-exchange chromatography and gel filtration on a column of bio-gel a-1.5m. the amino acid composition of lsp inclu ...19836643496
survey of insecticide resistance in blattella germanica (l.) from polish sea-going ships. 19836679458
[a model of cockroach control in a large area. ii. occurrence and distribution of blattella germanica and blatta orientalis in the district of potsdam (gdr)--an infestation analysis].blattella germanica and blatta orientalis occur in the district of potsdam (german democratic republic). b. germanica is the real cause of plague. up to now 2,734 building properties have been infested with cockroaches. b. germanica was found in 2,644 properties; b. orientalis was found in 20 properties and both were found in 70 properties. infestation with cockroaches increases with urbanisation. infestation is especially frequent in works canteens and similar rooms. outdoor distribution of coc ...19836859621
double minutes and other chromosomal aberrations in two malignant cell lines of the german cockroach blattella germanica.structural anomalies of mitotic chromosomes from two tumorigenic cell lines of the german cockroach (blattella germanica) is described. aberrations, such as unpairable marker chromosomes, double minutes, di- and tricentrics, ring chromosomes, tri- and quadriradials, and chromosome and chromatid gaps and breaks, were observed in varying proportions. this study reports that the double minute chromosomes (dms) are associated with insect tumor cells, similar to the findings in both murine and human ...19836883296
[usefulness of bendiocarb in the control of the german cockroach blattella germanica (l.) on ships]. 19827184132
allergy to cockroach antigens in asthmatic patients.cockroach allergy was investigated in a group of 56 patients with atopic bronchial asthma (37 men and 19 women with ages ranging from 16 to 65) all allergic to house dust antigen. in all patients, both intracutaneous tests and bronchial provocation tests were performed with cockroach antigen prepared from the species most common in poland, blattella germanica and blatta orientalis. positive skin reactions to cockroach antigen were found in 17 patients while an immediate bronchoconstrictive respo ...19817349028
efficacy of propoxur insecticide strip use in a laboratory animal facility.a non-fumigant, controlled-release, impregnated strip (insecticide strip) was evaluated for effectiveness against cockroaches (blatella germanica) in a conventional animal facility. a substantial initial roach mortality was observed in treated rooms. continued use of the insecticide strip assisted in the control of the cockroach population and did not affect plasma cholinesterase levels of exposed laboratory animals.19807464034
kdr-type resistance in insects with special reference to the german cockroach, blattella germanica.the phenomenon of knockdown resistance (kdr) was first noted in the housefly (musca domestica), and has subsequently be found (i.e. kdr-type resistance) in several other insect pests including the german cockroach (blattella germanica). this type of resistance causes insensitivity of the nervous system to pyrethroids, ddt and a limited number of sodium channel neurotoxins. in the german cockroach, kdr-type resistance is incompletely recessive, monogenic and not sex linked or due to cytoplasmic f ...19947553340
biologic potency and immunoblotting studies of extracts of three cockroach species.although cockroaches are responsible for severe allergic disease, cockroach extracts are not yet standardized by the id50eal method, nor have the allergens been completely characterized.19957583846
dna sequence and site of mutation of the gaba receptor of cyclodiene-resistant red flour beetle, tribolium castaneum.using polymerase chain reactions (pcr) on cdna, the dna sequence of a membrane spanning region of a gaba receptor of the red flour beetle, tribolium castaneum was identified. the deduced amino acid sequence indicates that its basic structure is similar to the gaba receptor of rdl type subunits of drosophila melanogaster and of blattella germanica. particularly conserved are m1, m2 and m3 segments. within this 146 amino acid stretch, the gaba receptor from the red flour beetle differed from corre ...19957613764
[testing effectiveness of selected bio-insecticides for reducing the population of cockroaches (blattella germanica l.)].the usefulness was tested of the biological insecticides containing b. thuringiensis available in the market in the eradication of cockroaches. thuridan was the most effective out of the tested insecticides in reducing the number of the insects. its higher activity in relation to thuricide was evident at all tested concentrations. males were more sensitive than females. on the other hand, no significant response of cockroaches to m-one preparation was noted, since the per cent of dying insects w ...19947878338
[assessment of resistance to permethrin of german cockroaches (blattella germanica l.) caught in poland].resistance to permethrin was tested in cockroaches caught in health service institutions in various parts of poland. the tests were carried out by the contact method as recommended by the who. the density of the insecticide was 20 mg/m2. the experiment was done only on male insects, calculating the values of lt50 and lt90 in insect groups caught in various localities. these values were compared with lt50 and lt90 values in sensitive insects exposed to the insecticide, and the resistance indices ...19947878339
sensitization to cockroach allergens of asthma patients in japan.to evaluate the role of allergens from periplaneta fuliginosa, which is the most predominant cockroach species in homes in tokyo areas, for asthma sensitization, we measured specific ige antibodies to two cockroaches, p. fuliginosa and blattella germanica, and to a mite, dermatophagoides farinae, in 171 sera from children with asthma by pharmacia's cap system. we found that 16% of the sera had anti-p. fuliginosa ige, whereas 9.9% had anti-b. germanica and 85% anti-d. farinae ige. further, we mea ...19947887806
3-hydroxy-3-methylglutaryl-coenzyme-a synthase from blattella germanica. cloning, expression, developmental pattern and tissue expression.insects do not synthesize cholesterol; the 3-hydroxy-3-methylglutaryl-coenzyme-a (hmg-coa) produced by hmg-coa synthase is transformed to mevalonate by hmg-coa reductase for the production of non-sterol isoprenoids, which are essential for growth and differentiation. to understand the regulation and developmental role of hmg-coa synthase, we have cloned a 1658 bp cdna that encompasses the entire transcription unit of the hmg-coa synthase gene from the cockroach blattella germanica. this cdna clo ...19937901012
[laboratory studies of the usefulness of biopreparation dipel in reducing the german cockroach (blattella germanica l.) population].in the studies conducted for finding of biological factors effectively controlling insects of hygienic importance german cockroaches were subjected to the action of an insecticide containing spores of bacillus thuringiensis var. kurstaki. one gram of the insecticide contained 25 miliard (us billion) spores with protein endotoxin crystals. the insects were given drinking water and food containing 1.0%, 0.5%, 0.25%, 0.125% of the initial preparation. the experiment was carried out on 1800 cockroac ...19938016546
[hyp3]met-callatostatin. identification and biological properties of a novel neuropeptide from the blowfly calliphora vomitoria.a novel, hydroxyproline-containing neuropeptide, gly-pro-hyp-tyr-asp-phe-gly-met-nh2, designated [hyp3]met-callatostatin, has been identified from extracts of heads of the blowfly calliphora vomitoria. the peptide is a naturally occurring hydroxylate analogue of met-callatostatin, a previously identified allatostatin-like peptide, and is present to the extent of 20% of the nonhydroxylated form. in bioassays, both forms of the peptide show allatostatic activity by inhibiting juvenile hormone synt ...19948063725
effects of three synergists on pyrethroid resistance in the german cockroach (dictyoptera: blattellidae).the effects of three synergists on the level of resistance to eight pyrethroids were examined in field-collected populations of the german cockroach, blattella germanica (l.). resistance to allethrin was not affected by piperonyl butoxide (pbo), (2-(2-ethylhexyl)-3a,4,7,7a-tetrahydro-4,7-methano-1h-isoindole-1,3(2h)- dione (mgk 264), or s,s,s-tributyl phosphorotrithioate (def). resistance to pyrethrins also was not affected by these synergists in the same or similar strains but was negated almos ...19948071470
vector potential of the german cockroach in dissemination of pseudomonas aeruginosa.survival time studies of pseudomonas aeruginosa in cockroaches (blattella germanica) were carried out under standard laboratory conditions. cockroaches were fed with graded doses (10(2), 10(5) or 10(7) per insect) of p. aeruginosa. depending on the excretion of p. aeruginosa the cockroaches were categorized as non-excretors, intermittent excretors or continuous excretors. when a dose of 10(2) p. aeruginosa was used all the insects were non-excretors but with doses of 10(5) or 10(7) per insect, p ...19938095950
characterization of toxin iii of the scorpion leiurus quinquestriatus quinquestriatus: a new type of alpha-toxin highly toxic both to mammals and insects.the primary structure of toxin iii of leiurus quinquestriatus quinquestriatus (lqq iii) was elucidated by automatic edman degradation of the reduced and s-carboxymethylated protein and derived tryptic peptides. like other scorpion toxins that are active on sodium channels, lqq iii, consisting of 64 amino acids, is a 7 kda single-chain polypeptide crosslinked by four disulfide bridges. it belongs to the alpha-toxin group, as judged by competition experiments with 125i aah ii for binding to rat br ...19938167952
purification and characterization of vitellin-2 from the ovary of the american cockroach, periplaneta americana.two vitellins (vn-1 and vn-2) were identified in cockroach ovary. vn-2 was purified by ion-exchange chromatography using deae cellulose and sepharose cl-6b, and gel filtration using sephadex g-200 and sepharose 4b. vn-2 comprises three subunits with molecular weights of 102, 72 and 40 kda. vn-2 contains diacylglycerol and triacylglycerol; their fatty acids are mostly lauric acid and palmitoleic acid. vn-2 also has phosphatidylcholine and phosphatidylethanolamine but no sphingomyelin. vn-2 contai ...19948205388
in vitro translation of rna from the german cockroach blattella germanica.the translation of mrna within total rna of german (blattella germanica) cockroaches was performed using a rabbit reticulocyte lysate system. analysis of the translation products by sds-page and combined autoradiography revealed several synthesized proteins with apparent molecular weights ranging from 20 kd to 110 kd. sds-page/western blotting of non-radiolabeled translation products and incubation with human serum with ige to cockroach allergens showed the presence of a 36 kd and 50 kd allergen ...19938272080
toxicity of the venom from nasonia vitripennis (hymenoptera: pteromalidae) toward fly hosts, nontarget insects, different developmental stages, and cultured insect cells.a venom preparation from nasonia vitripennis, a wasp ectoparasitoid of fly pupae, was assayed for lethality in different stages of insects representing ten different orders and in cultured insect cells. in most cases, the motor activity of the injected insects remained completely normal for 1-2 days after the injection and displayed none of the symptoms of paralysis commonly reported for venoms of the hymenoptera. a natural host, the flesh fly sarcophaga bullata, was highly sensitive in the pupa ...19938342173
molecular cloning, developmental pattern and tissue expression of 3-hydroxy-3-methylglutaryl coenzyme a reductase of the cockroach blattella germanica.in insects, 3-hydroxy-3-methylglutaryl coenzyme a reductase (hmg-coa) synthesizes mevalonate for the production of nonsterol isoprenoids, which are essential for growth and differentiation. to understand the regulation and developmental role of hmg-coa reductase, we have cloned a full-length hmg-coa reductase cdna from the cockroach blattella germanica. this cdna clone was isolated using as a probe a partial cdna of b. germanica hmg-coa reductase, amplified using the polymerase chain reaction. t ...19938477698
quality of housing and allergy to cockroaches in the dominican republic.fifty-one atopic asthmatic and/or allergic rhinitic children and 23 nonatopic control from santo domingo, the dominican republic, were skin tested with an extract mix of three cockroach species (blattella germanica, blatta orientalis, and periplaneta americana). sixteen percent of the atopics and none of the nonatopics demonstrated positive immediate skin reactions to the cockroach mix (chi 2 = 4.05, p = 0.04). hypersensitivity was correlated with the quality of the homes; 22% (8/36) of the atop ...19968527953
cloning of cockroach allergen, bla g 4, identifies ligand binding proteins (or calycins) as a cause of ige antibody responses.an allergen cloned from a blattella germanica (german cockroach) cdna library, encoded a 182-amino acid protein of 20,904 da. this protein, designated b. germanica allergen 4 (bla g 4), was expressed as a glutathione s-transferase fusion protein in escherichia coli and purified by affinity chromatography and high-performance liquid chromatography. the prevalence of serum ige antibody to recombinant bla g 4 in 73 cockroach allergic patients with asthma ranged from 40% (antigen binding radioimmuno ...19958537384
[laboratory examination of the effectiveness of new biological preparations for reducing populations of cockroaches (blattella germanica l.)].the usefulness of new commercial products of biological origin (biobit, novo skeetal and novodor) was tested in cockroaches (blattella germanica l.) control. biobit proved to be the most effective in reduction of cockroaches population. its higher activity against cockroaches was observed in all concentrations tested. females were more sensitive for all tested products than males.19958552970
[cockroach allergy: a study of its prevalence using skin tests with commercial extracts].cockroaches have been increasingly recognized as an important source of indoor allergens. in this study we assessed the prevalence of cockroach sensitization among an outpatient population observed at our department (155 patients with a mean age of 29 +/- 12 years), based on skin "prick" tests with four commercial cockroach extracts along with a common battery of standardized inhalant allergens. we found a positive wheal to at least one of these four extracts in 27 patients: 26 (96.2%) to blatta ...19958579009
a recombinant insect-specific alpha-toxin of buthus occitanus tunetanus scorpion confers protection against homologous mammal toxins.we have constructed a cdna library from venom glands of the scorpion buthus occitanus tunetanus and cloned a dna sequence that encodes an alpha-toxin. this clone was efficiently expressed in escherichia coli as a fusion protein with two ig-binding (z) domains of protein a from staphylococcus aureus. after cnbr treatment of the fusion protein and hplc purification, we obtained approximately 1 mg recombinant apha-toxin/l bacterial culture. the toxin, called bot xiv, displays no toxicity towards ma ...19968706664
allergen-specific ige levels and mite allergen exposure in children with acute asthma first seen in an emergency department and in nonasthmatic control subjects.sensitization to allergens has been shown to be a risk factor for adults with acute asthma first seen in the emergency department.19968757201
isolation and characterization of a clone encoding a major allergen (bla g bd90k) involved in ige-mediated cockroach hypersensitivity.previous studies have established that atopic individuals living in cockroach-infested housing become sensitized to cockroach aeroallergens and produce ige antibodies to a variety of proteins. we describe the isolation of a complementary dna clone from an expression library, constructed with messenger rna from german (blattella germanica) cockroaches, which encodes a major allergen involved in mediating cockroach hypersensitivity. approximately 0.2% of the clones from a lambda zap xr cdna librar ...19968765832
gongylonematiasis in the common marmoset (callithrix jacchus).two cases of gongylonematiasis in common marmosets of two research facilities in germany are reported. the helminthiasis was transmitted from colony a to colony b by one infected female and within colony b by cockroaches (blatella germanica). four of 40 cockroaches examined in colony b were infected with rhabditiform larvae. clinical signs of disease in the infected animals consisted of intense itching and scratching of the edematous and slightly hyperemic perioral tissues. histologically the ad ...19968799930
host specificity of gregarina blattarum von siebold, 1839 (apicomplexa: eugregarinida) among five species of domiciliary cockroachesthe host specificity of gregarina blattarum was evaluated among five species of domiciliary cockroaches: blatella germanica, supella longipalpa, blatta orientalis, periplaneta americana, and periplaneta fuliginosa. third- and fourth-instar nymphs were allowed to feed on crushed dog kibble contaminated with g. blattarum oocysts. cockroaches were killed 8 days postinfection and examined for parasites. gregarina blattarum infections were observed in all experimentally infected replications of b. ge ...19968812602
allergy to cockroaches in patients with asthma and rhinitis in an urban area (madrid).previous studies have established that cockroach allergens are important sensitizing agents in the induction of rhinitis and asthma principally in urban areas. this study was undertaken to assess skin test reactivity and specific ige antibody reactivity to extracts of periplaneta americana (p.a.), blattella germanica (b.g.), b. orientalis (b.o.), and a fecal extract of b. germanica (feb.g.) in a group of patients with rhinitis and asthma living in an urban area in europe. we examined clinical ch ...19968874663
[susceptibility of cockroaches blattella germanica l. collected from hospitals to selected pyrethroid and carbamate insecticides].the resistance to four pyrethroid insecticides: permethrin, deltamethrin, cypermethrin, etofenprox, and to two carbamate insecticides--bendiocarb, propoxur was investigated on field strains of german cockroaches (blatella germanica l.) caught in hospitals from various parts of poland. the tests were carried out only on males by the contact method recommended by the who. the resistance was evaluated on the basis of lt50s and resistance ratios (rrs). the tested fields strains showed high or modera ...19969026900
[studies of the usefulness of beauveria bassiana for eradication of cockroaches (blattella germanica l.)].the ability of killing cockroaches (blattella germanica l.) by various strains of the mushroom beauveria bassiana was studied, including also strains obtained by passaging. the study was conducted using adult insects, of varying age and sex, derived from laboratory cultures or caught in hospital rooms. the obtained results pointed out differences in the pathogenicity of b. bassiana strains for the studied populations of insects. the per cent of mortality among the insects depended on the pathoge ...19969026901
cockroach allergen in house dust.the cockroach allergen (bla g 1) content was determined in the floor dust of 46 homes with recent cockroach extermination in amsterdam, the netherlands. ige antibodies to blattella germanica, house-dust mite, cat dander, dog dander, and a mixture of molds were determined in venous blood samples of 46 children (4-12 years) and one of their biologic parents (24-54 years). specific ige to cockroach was also determined in a sample of the general population studied in a previous case-control study, o ...19979188932
cross-reactivity between ige-binding proteins from anisakis, german cockroach, and chironomids.anisakis simplex larvae parasitize animals used as seafood and can produce a specific immune response in man. the ingestion of seafood contaminated with stage three of a. simplex larvae can induce a specific ige response with clinical symptoms, usually urticaria, even if the fish is cooked before ingestion and the invasive infestation power destroyed by heating. our preliminary studies showed a strong association of a. simplex sensitization with ascaris lumbricoides, daphnia, chironomid spp., at ...19979201362
induction of ige antibody responses by glutathione s-transferase from the german cockroach (blattella germanica).we report that a major 23-kda allergen from german cockroach (blattella germanica) is a glutathione s-transferase (ec 2.5.1.18; gst). natural b. germanica gst, purified from cockroach body extracts by glutathione affinity chromatography, and recombinant protein expressed in escherichia coli using the pet21a vector, showed excellent ige antibody binding activity. b. germanica gst caused positive immediate skin tests in cockroach-allergic patients using as little as 3 pg of recombinant protein. th ...19979252418
catalytic properties of recombinant 3-hydroxy-3-methylglutaryl coenzyme a synthase-1 from blattella germanica.blattella germanica is the first organism in which two cytosolic hmg-coa synthase genes have been described: hmgs-1 (martínez-gonzález et al., 1993b) and hmgs-2 (buesa et al., 1994). the hmgs-1 gene showed special features, which led us to characterize the kinetic properties of the enzyme it encodes. here we report the expression of recombinant hmgs-1, the protocol of enzyme purification, and the measurement of kinetic parameters. the k(m) for acetyl-coa is 15.2 microm and the ki for the other s ...19979304791
[laboratory determination of the activity of insecticidal fungus paecilomyces farinosus in reducing the numbers of cockroaches blattella germanica l].the strains of p. farinosus capable of killing the cockroach blattella germanica l. were selected. the experiment was carried out using mature insects in age groups and sex groups. the results showed differences in the insecticidal capability of the tested strains in this population of insects. strains 5, l, p of p. farinosus were accepted as possibly useful against blattella since at relatively low concentration of spores they produced a high mortality of the insects.19979381075
[preliminary evaluation of the incidence and control of insects--pest control in polish hospitals].the evaluation of the infestation and methods of insect disease vectors control in 748 hospitals in poland in the period of 1990 to 1995 were done. the insect species, places of their occurrence and control agents were analysed. blattella germanica l. occurred most frequently (71% hospitals). blatta orientalis and monomorium pharaonis were found in 40% and 17% hospitals respectively. kitchens, laundries and baths were most infested. sometimes insects were found also in central sterilization unit ...19979432707
aspergillosis in german cockroach blattella germanica (l.) (blattoidea: blattellidae).natural infection of aspergillus flavus was observed in adults of blattella germanica. though the adult insects exhibited no external symptoms, they became hypoactive and later died. the dead and experimentally infected insects repeatedly yielded aspergillus flavus in culture on czapek's medium. direct microscopic observation of the tissues of infected insects revealed fungal material. the blood films stained with giemsa stain showed granulocytes (grs) engulfing fungal hyphae. a remarkable incre ...19979549100
indoor allergens and longitudinal fev1 decline in older adults: the normative aging study.we investigated the relationship between home allergen exposure and decline in fev1 in 10 asthmatic and 30 randomly selected, age-matched, nonasthmatic participants in the normative aging study. we defined asthma as subject-reported wheezing apart from colds, with either a physician's diagnosis of asthma or a methacholine pd20 fev1 of 8.6 micromol or less. we examined the relationship between the annual decline in fev1 and the concentrations of the cockroach (blattella germanica) allergens bla g ...19989648697
cloning of the american cockroach cr-pii allergens: evidence for the existence of cross-reactive allergens between species.previously, we have identified the 28 and 32 kd proteins as additional important allergens from the american cockroach (periplaneta americana) cr-pii allergenic fraction.19989648712
evaluation of effect of metarhizium anisopliae on reduction in the number of blattella germanica l.estimates were given of effectiveness of infestation of b. germanica l. by m. anisopliae strains originating from different regions of poland. the investigated strains caused high mortality among experimental insects. especially effective in reducing numbers of b. germanica were strains browsk and pruszyn, which even at a low density of spores caused high mortality of the test insects.19989734233
use of random amplified polymorphic dna (rapd) for generating specific dna probes for oxyuroid species (nematoda).random amplified dna markers (rapd; williams et al., 1990) were used to obtained specific rapd fragments characterising different species of oxyuroids. we tested six species of worms parasitizing vertebrates or invertebrates: passalurus ambiguus rudolphi, 1819, parasite of leporids; syphacia obvelata (rudolphi, 1802) seurat, 1916, a parasite of rodents; blatticola blattae (graeffe, 1860) chitwood, 1932 parasite of the cockroach blattella germanica; hammerschmidtiella diesingi (hammerschmidt, 183 ...19989754296
sensitization to cockroach allergens in a sample from the urban population living in naples (southern italy).the aim of this study was to evaluate the prevalence of allergic sensitization to cockroach allergens in a group of atopic patients living in the urban area of naples. four hundred and fourteen consecutive patients were submitted to the following diagnostic procedures: anamnestic data; clinical examination; and skin prick test by using a standardized pricker and allergenic extracts, one of which contained a mix of whole bodies of blattella orientatis, blattella germanica and periplaneta american ...19989777540
novel allergen structures with tandem amino acid repeats derived from german and american cockroach.cockroaches produce potent allergens that are an important cause of asthma. the two principal domiciliary cockroach species, blattella germanica and periplaneta americana, secrete major allergens, bla g 1 and per a 1. here, we report the molecular cloning of three bla g 1 cdna clones, which showed 70% amino acid sequence identity with per a 1. plaque immunoassays with human ige antibodies or murine monoclonal antibodies showed that these allergens were antigenically cross-reactive. the bla g 1 s ...19989804858
noxious components of venom from the pupa-specific parasitoid pimpla hypochondriacavenom from the endoparasitoid wasp pimpla hypochondriaca induced a lethal paralysis when injected into the hemocoel of larval, pupal, and adult stages of the tomato moth lacanobia oleracea and was also toxic to the adult stages of the housefly musca domestica and the cockroach blatella germanica. heating the venom to 58 degreesc did not reduce its potency against m. domestica. phenoloxidase activity was detected in diluted venom using dihydroxyphenylalanine as the substrate and occurred independ ...19999878292
characterization of a new iridovirus isolated from crickets and investigations on the host rangetypical signs of an iridovirus infection were observed in two species of fatally diseased crickets, gryllus campestris l. and acheta domesticus l. (orthoptera, gryllidae). the infection was manifested by hypertrophy and bluish iridescence of the affected fat body cells. electron microscope investigations led to the identification of a new iridovirus, which was termed cricket iridovirus (criv). in negatively stained preparations the size of the icosahedral virus particles ranged from 151 nm (side ...19999878293
ultrastructural investigation of the spore-forming protist nephridiophaga blattellae in the malpighian tubules of the german cockroach blattella germanica.multinuclear plasmodia of the sporogenic protist nephridiophaga blattellae are found intracellularly and in the lumen of the malpighian tubules of the german cockroach blattella germanica. spore formation occurs only in the lumen. during sporogony, about 10-35 spores measuring 5.5x3.2 microm are endogenously formed within a plasmodium. sporoblasts arise by the fusion of cisternae of the endoplasmic reticulum into a double membranous wall, which encloses a generative nucleus plus a portion of cyt ...19999951966
the molecular evolution of the allatostatin precursor in cockroaches.allatostatins (asts) of the tyr/phe-xaa-phe-gly leu/ile-nh2 family are a group of insect neuropeptides that inhibit juvenile hormone biosynthesis by the corpora allata. we have obtained genomic dna sequences that specify the preproallatostatin precursor for the cockroaches, blatta orientalis, blattella germanica, blaberus (cranufer and supella longipalpa. the sequences obtained are similar to those of diploptera punctata and periplaneta americana reported previously. the precursors of all these ...199910098619
experimental aspergillosis in the german cockroach blattella germanica: a histopathological study.inoculation of a spore suspension of aspergillus flavus in the haemocoel of the german cockroach (blatella germanica) resulted in large-scale invasion of various internal organs as seen in tissue sections stained with a fungal stain. the organs affected were the alimentary canal, fat bodies, muscle fibres, malpighian tubules and the cerebral neurosecretory cells. no invasion of the trachea and tracheoles was noted.199810205882
immunologic characterization of a recombinant american cockroach (periplaneta americana) per a 1 (cr-pii) allergen.previously, we have identified several per a 1 (cr-pii) allergens from a deltagt22a cdna library of periplaneta americana. this study aimed to sequence clone c42 and determine its molecular and antigenic properties.199910221434
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