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the binding of mouse hybridoma and human ige antibodies to the major fecal allergen, der p i, of dermatophagoides pteronyssinus. relative binding site location and species specificity studied by solid-phase inhibition assays with radiolabeled antigen.the relative binding site location and species specificity of 19 mouse hybridoma antibodies, produced in four laboratories, to dermatophagoides pteronyssinus major fecal allergen, der p i, was studied by using immobilized mab and inhibitions of radiolabeled ag binding. four mab groups were defined, within which 4, 6, 8, and 5 mab, respectively, cross-inhibited each other. five mab were members of both group 2 and 3, demonstrating a considerable overlap of epitopes between the corresponding antib ...19883372999
collection of house dust for analysis of mite allergens. allergen-reducing effect of a self-administered procedure.to assess allergen-reducing effect of dust collection from mattresses, patients were asked to vacuum clean the entire surface of their mattresses for 5 min with their household cleaners at specified intervals ranging from 1-21 days. ten patients performed four collections, nine patients only two. amounts of dust, concentrations and amounts of major allergens from dermatophagoides pteronyssinus (dp42), dermatophagoides microceras (dm6), and dermatophagoides farinae (df6) were determined. the firs ...19863532859
taxonomic and immunochemical identification of two house dust mites dermatophagoides farinae and dermatophagoides microceras. 19873819223
demonstration of close physicochemical similarity and partial immunochemical identity between the major allergen, dp42, of the house dust mite, d. pteronyssinus and corresponding antigens of d. farinae (df6) and d. microceras (dm6).house dust mite antigens df6 and dm6, corresponding to dermatophagoides pteronyssinus (dp) antigen 42 (= p1), were purified from cultures of dermatophagoides farinae (df) and dermatophagoides microceras (dm) using hydrophobic interaction chromatography in combination with gel filtration or acetone precipitation. purified antigens from the three mite species (dp42, df6 and dm6) were similar regarding molecular weight by gel filtration (d. pteronyssinus: 17,000, d. farinae: 17,000 and d. microcera ...19863941011
specific ige to 5 different major house dust mites among asthmatic children.asthma is one of the most commonly occurring manifestation of allergy in taiwan. sensitivity to house dust mites is closely related to childhood asthma. this study was designed to investigate sensitized rates and average concentrations of specific ige antibodies to 5 major house dust mites (hdms) among asthmatic children. a total of 93 asthmatic children aged from 3 to 15 years were enrolled to measure their specific ige concentrations in response to 5 different species of mites: dermatophagoide ...200212607482
distribution of allergens in children with different atopic disorders in central taiwan.allergic disorders, including asthma, allergic rhinitis, and eczema, are the most common chronic childhood diseases. exposure to house dust mites (hdms) can exacerbate allergic disorders in sensitized individuals. the data for sensitization to hdms and other frequent allergens amongst atopic children in taiwan is limited. we studied 498 children (aged 2-16 years) with atopy in central taiwan with cap testing (cationic antimicrobial protein system). our results revealed a high prevalence of sensi ...200617078465
comparative mites and cockroaches sensitization study in three cities of mexico.in mexico there are few research papers dealing with mites and cockroaches sensibilizing in patients suffering from respiratory allergies and even less susceptibility comparative studies to the same between cities having different climatic and geographic characteristics.200819157220
significance of specific igg against sensitizing antigens in extrinsic allergic alveolitis: serological methods in eaa.the aim of our study is to find differences in igg in sera of potentially exposed and nonexposed individuals and to detect differences in concentrations of specific serum igg among subjects with and without eaa. seventy-two patients being followed for suspected interstitial lung disease were included. specific igg in sera were established by immunocap. serum concentrations of aspergillus fumigatus, candida albicans igg and mixture of moulds igg were higher in subjects with exposure to relevant i ...201121924863
alleviation of dermatophagoides microceras-induced allergy by an immunomodulatory protein, fip-fve, from flammulina velutipes in mice.asthma is a major public health concern. its greatest risk factor is house dust mite (hdm). dermatophagoides microceras (der m) is a type of hdm, and in central taiwan, there is approximately 80% prevalence of sensitization to der m. fip-fve is a fungal immunomodulatory protein (fip) isolated from the fungus flammulina velutipes, and exhibits anti-inflammatory properties. to investigate whether fip-fve affects der m-induced asthma and inflammation, we evaluated hyper-responsiveness (ahr), pathol ...201525209380
mite species identification in the production of allergenic extracts for clinical use and in environmental samples by ribosomal dna amplification.the identification of allergy-causing mites is conventionally based on morphological characters. however, molecular taxonomy using ribosomal dna (rdna) may be particularly useful in the analysis of mite cultures and purified mite fractions in the production of allergenic extracts. full-length internal transcribed spacers (its1 and its2) were obtained from dermatophagoides farinae, dermatophagoides pteronyssinus, dermatophagoides microceras and euroglyphus maynei (astigmata: pyroglyphidae), glycy ...201424617319
environmental assessment of dermatophagoides mite-allergen levels in sweden should include der m 1.the major allergen of dermatophagoides microceras, der m 1, as well as the allergens of d. pteronyssinus and d. farinae, der p 1 and der f 1, were analyzed in the homes of 111 asthmatic children in three climatic regions in sweden. the numbers and species of mites were determined by microscopy, and circulating ige antibodies against mites were measured. der f 1 was the predominant house-dust-mite (hdm) allergen, der p 1 the least often found, and der m 1 represented 31% of the allergen load. how ...19989700039
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